LOCUS       DAJTMX010000001       109692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTMX010000001 DAJTMX010000000
VERSION     DAJTMX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 109692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.001"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..697)
                     /locus_tag="OO145_000001"
     CDS             complement(<1..697)
                     /locus_tag="OO145_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5465284.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLA"
     gene            865..1083
                     /locus_tag="OO145_000002"
                     /pseudo
     CDS             865..1083
                     /locus_tag="OO145_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            1101..2429
                     /gene="dsdA"
                     /locus_tag="OO145_000003"
     CDS             1101..2429
                     /gene="dsdA"
                     /locus_tag="OO145_000003"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5465285.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGRIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(2537..4075)
                     /gene="emrY"
                     /locus_tag="OO145_000004"
     CDS             complement(2537..4075)
                     /gene="emrY"
                     /locus_tag="OO145_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5465286.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(4075..5238)
                     /gene="emrK"
                     /locus_tag="OO145_000005"
     CDS             complement(4075..5238)
                     /gene="emrK"
                     /locus_tag="OO145_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5465287.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            5654..6268
                     /gene="evgA"
                     /locus_tag="OO145_000006"
     CDS             5654..6268
                     /gene="evgA"
                     /locus_tag="OO145_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5465288.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            6273..9866
                     /gene="evgS"
                     /locus_tag="OO145_000007"
     CDS             6273..9866
                     /gene="evgS"
                     /locus_tag="OO145_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5465289.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(9922..11067)
                     /gene="yfdE"
                     /locus_tag="OO145_000008"
     CDS             complement(9922..11067)
                     /gene="yfdE"
                     /locus_tag="OO145_000008"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5465290.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(11141..12085)
                     /gene="yfdV"
                     /locus_tag="OO145_000009"
     CDS             complement(11141..12085)
                     /gene="yfdV"
                     /locus_tag="OO145_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5465291.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(12155..13849)
                     /gene="oxc"
                     /locus_tag="OO145_000010"
     CDS             complement(12155..13849)
                     /gene="oxc"
                     /locus_tag="OO145_000010"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5465292.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(13903..15153)
                     /gene="frc"
                     /locus_tag="OO145_000011"
     CDS             complement(13903..15153)
                     /gene="frc"
                     /locus_tag="OO145_000011"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5465293.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(15666..16289)
                     /locus_tag="OO145_000012"
     CDS             complement(15666..16289)
                     /locus_tag="OO145_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5465294.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            16586..16861
                     /locus_tag="OO145_000013"
     CDS             16586..16861
                     /locus_tag="OO145_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465295.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(16938..17180)
                     /locus_tag="OO145_000014"
     CDS             complement(16938..17180)
                     /locus_tag="OO145_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5465296.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            17533..18453
                     /gene="lpxP"
                     /locus_tag="OO145_000015"
     CDS             17533..18453
                     /gene="lpxP"
                     /locus_tag="OO145_000015"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5465297.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            18808..18879
                     /gene="ypdK"
                     /locus_tag="OO145_000016"
     CDS             18808..18879
                     /gene="ypdK"
                     /locus_tag="OO145_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5465298.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(18944..20182)
                     /gene="alaC"
                     /locus_tag="OO145_000017"
     CDS             complement(18944..20182)
                     /gene="alaC"
                     /locus_tag="OO145_000017"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5465299.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            20559..22045
                     /locus_tag="OO145_000018"
                     /pseudo
     CDS             20559..22045
                     /locus_tag="OO145_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            22060..22794
                     /gene="ypdB"
                     /locus_tag="OO145_000019"
     CDS             22060..22794
                     /gene="ypdB"
                     /locus_tag="OO145_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5465300.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            22807..23664
                     /locus_tag="OO145_000020"
                     /pseudo
     CDS             22807..23664
                     /locus_tag="OO145_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(23667..26162)
                     /gene="ptsP"
                     /locus_tag="OO145_000021"
     CDS             complement(23667..26162)
                     /gene="ptsP"
                     /locus_tag="OO145_000021"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5465301.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(26187..27224)
                     /gene="ypdE"
                     /locus_tag="OO145_000022"
     CDS             complement(26187..27224)
                     /gene="ypdE"
                     /locus_tag="OO145_000022"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5465302.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(27224..28307)
                     /gene="ypdF"
                     /locus_tag="OO145_000023"
                     /pseudo
     CDS             complement(27224..28307)
                     /gene="ypdF"
                     /locus_tag="OO145_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(28322..29569)
                     /locus_tag="OO145_000024"
     CDS             complement(28322..29569)
                     /locus_tag="OO145_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5465303.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(29591..29917)
                     /locus_tag="OO145_000025"
     CDS             complement(29591..29917)
                     /locus_tag="OO145_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5465304.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(30136..31101)
                     /gene="glk"
                     /locus_tag="OO145_000026"
     CDS             complement(30136..31101)
                     /gene="glk"
                     /locus_tag="OO145_000026"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5465305.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            31305..32561
                     /locus_tag="OO145_000027"
     CDS             31305..32561
                     /locus_tag="OO145_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5465306.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            32676..33002
                     /locus_tag="OO145_000028"
     CDS             32676..33002
                     /locus_tag="OO145_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5465307.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(33143..34381)
                     /locus_tag="OO145_000029"
     CDS             complement(33143..34381)
                     /locus_tag="OO145_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5465308.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            34717..35919
                     /gene="nupC"
                     /locus_tag="OO145_000030"
     CDS             34717..35919
                     /gene="nupC"
                     /locus_tag="OO145_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5465309.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(35969..38081)
                     /locus_tag="OO145_000031"
                     /pseudo
     CDS             complement(35969..38081)
                     /locus_tag="OO145_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(38289..38364)
                     /locus_tag="OO145_000032"
     tRNA            complement(38289..38364)
                     /locus_tag="OO145_000032"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38329..38331),aa:Ala,seq:ggc)
     gene            complement(38404..38479)
                     /locus_tag="OO145_000033"
     tRNA            complement(38404..38479)
                     /locus_tag="OO145_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38444..38446),aa:Ala,seq:ggc)
     gene            38700..39059
                     /locus_tag="OO145_000034"
     CDS             38700..39059
                     /locus_tag="OO145_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5465310.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            39061..39453
                     /locus_tag="OO145_000035"
     CDS             39061..39453
                     /locus_tag="OO145_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5465311.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(39505..40920)
                     /gene="gltX"
                     /locus_tag="OO145_000036"
     CDS             complement(39505..40920)
                     /gene="gltX"
                     /locus_tag="OO145_000036"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5465312.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            41179..41254
                     /locus_tag="OO145_000037"
     tRNA            41179..41254
                     /locus_tag="OO145_000037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41212..41214,aa:Val,seq:tac)
     gene            41299..41374
                     /locus_tag="OO145_000038"
     tRNA            41299..41374
                     /locus_tag="OO145_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41332..41334,aa:Val,seq:tac)
     gene            41379..41454
                     /locus_tag="OO145_000039"
     tRNA            41379..41454
                     /locus_tag="OO145_000039"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41412..41414,aa:Lys,seq:ttt)
     gene            41485..41610
                     /locus_tag="OO145_000040"
     ncRNA           41485..41610
                     /ncRNA_class="other"
                     /locus_tag="OO145_000040"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            41730..42071
                     /locus_tag="OO145_000041"
     CDS             41730..42071
                     /locus_tag="OO145_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5465313.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(42062..42988)
                     /locus_tag="OO145_000042"
     CDS             complement(42062..42988)
                     /locus_tag="OO145_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5465314.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            43078..44076
                     /locus_tag="OO145_000043"
     CDS             43078..44076
                     /locus_tag="OO145_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5465315.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(44073..44291)
                     /locus_tag="OO145_000044"
     CDS             complement(44073..44291)
                     /locus_tag="OO145_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5465316.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQHLKRPL"
     gene            complement(44293..46308)
                     /gene="ligA"
                     /locus_tag="OO145_000045"
     CDS             complement(44293..46308)
                     /gene="ligA"
                     /locus_tag="OO145_000045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5465317.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGIGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(46379..47365)
                     /gene="zipA"
                     /locus_tag="OO145_000046"
     CDS             complement(46379..47365)
                     /gene="zipA"
                     /locus_tag="OO145_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5465318.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            47595..48356
                     /gene="cysZ"
                     /locus_tag="OO145_000047"
     CDS             47595..48356
                     /gene="cysZ"
                     /locus_tag="OO145_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5465319.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            48541..49512
                     /gene="cysK"
                     /locus_tag="OO145_000048"
     CDS             48541..49512
                     /gene="cysK"
                     /locus_tag="OO145_000048"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5465320.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            49896..50153
                     /gene="ptsH"
                     /locus_tag="OO145_000049"
     CDS             49896..50153
                     /gene="ptsH"
                     /locus_tag="OO145_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5465321.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            50198..51925
                     /gene="ptsI"
                     /locus_tag="OO145_000050"
     CDS             50198..51925
                     /gene="ptsI"
                     /locus_tag="OO145_000050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5465322.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            51966..52475
                     /gene="crr"
                     /locus_tag="OO145_000051"
     CDS             51966..52475
                     /gene="crr"
                     /locus_tag="OO145_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5465323.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(52517..53368)
                     /gene="pdxK"
                     /locus_tag="OO145_000052"
                     /gene_synonym="thiJ"
     CDS             complement(52517..53368)
                     /gene="pdxK"
                     /locus_tag="OO145_000052"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5465324.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            53473..53847
                     /locus_tag="OO145_000053"
     CDS             53473..53847
                     /locus_tag="OO145_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5465325.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            53880..54603
                     /locus_tag="OO145_000054"
                     /pseudo
     CDS             53880..54603
                     /locus_tag="OO145_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(54807..55718)
                     /gene="cysM"
                     /locus_tag="OO145_000055"
     CDS             complement(54807..55718)
                     /gene="cysM"
                     /locus_tag="OO145_000055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5465326.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(55852..56949)
                     /gene="cysA"
                     /locus_tag="OO145_000056"
     CDS             complement(55852..56949)
                     /gene="cysA"
                     /locus_tag="OO145_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5465327.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(56939..57814)
                     /gene="cysW"
                     /locus_tag="OO145_000057"
     CDS             complement(56939..57814)
                     /gene="cysW"
                     /locus_tag="OO145_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5465328.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(57814..58647)
                     /gene="cysT"
                     /locus_tag="OO145_000058"
     CDS             complement(57814..58647)
                     /gene="cysT"
                     /locus_tag="OO145_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5465329.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(58647..59663)
                     /gene="cysP"
                     /locus_tag="OO145_000059"
     CDS             complement(58647..59663)
                     /gene="cysP"
                     /locus_tag="OO145_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5465330.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(59967..60758)
                     /gene="ucpA"
                     /locus_tag="OO145_000060"
     CDS             complement(59967..60758)
                     /gene="ucpA"
                     /locus_tag="OO145_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5465331.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(60945..61745)
                     /gene="murR"
                     /locus_tag="OO145_000061"
     CDS             complement(60945..61745)
                     /gene="murR"
                     /locus_tag="OO145_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5465332.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            61909..62805
                     /gene="murQ"
                     /locus_tag="OO145_000062"
     CDS             61909..62805
                     /gene="murQ"
                     /locus_tag="OO145_000062"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5465333.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62809..64233
                     /gene="murP"
                     /locus_tag="OO145_000063"
     CDS             62809..64233
                     /gene="murP"
                     /locus_tag="OO145_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5465334.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(64413..65312)
                     /gene="yfeX"
                     /locus_tag="OO145_000064"
     CDS             complement(64413..65312)
                     /gene="yfeX"
                     /locus_tag="OO145_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5465335.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(65408..65983)
                     /locus_tag="OO145_000065"
     CDS             complement(65408..65983)
                     /locus_tag="OO145_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5465336.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(66044..66493)
                     /locus_tag="OO145_000066"
     CDS             complement(66044..66493)
                     /locus_tag="OO145_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5465337.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(66480..66905)
                     /locus_tag="OO145_000067"
     CDS             complement(66480..66905)
                     /locus_tag="OO145_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5465338.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            67119..67988
                     /gene="amiA"
                     /locus_tag="OO145_000068"
     CDS             67119..67988
                     /gene="amiA"
                     /locus_tag="OO145_000068"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5465339.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            67992..68891
                     /gene="hemF"
                     /locus_tag="OO145_000069"
     CDS             67992..68891
                     /gene="hemF"
                     /locus_tag="OO145_000069"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5465340.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(68897..69949)
                     /gene="eutR"
                     /locus_tag="OO145_000070"
                     /pseudo
     CDS             complement(68897..69949)
                     /gene="eutR"
                     /locus_tag="OO145_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(69995..70495)
                     /gene="eutK"
                     /locus_tag="OO145_000071"
     CDS             complement(69995..70495)
                     /gene="eutK"
                     /locus_tag="OO145_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5465341.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(70508..71167)
                     /gene="eutL"
                     /locus_tag="OO145_000072"
     CDS             complement(70508..71167)
                     /gene="eutL"
                     /locus_tag="OO145_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5465342.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            72349..73140
                     /locus_tag="OO145_000073"
     CDS             72349..73140
                     /locus_tag="OO145_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465343.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            73860..74255
                     /locus_tag="OO145_000074"
     CDS             73860..74255
                     /locus_tag="OO145_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5465344.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            74273..74536
                     /locus_tag="OO145_000075"
     CDS             74273..74536
                     /locus_tag="OO145_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465345.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            74559..75101
                     /locus_tag="OO145_000076"
     CDS             74559..75101
                     /locus_tag="OO145_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465346.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            75240..75527
                     /locus_tag="OO145_000077"
     CDS             75240..75527
                     /locus_tag="OO145_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465347.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            75566..75772
                     /locus_tag="OO145_000078"
     CDS             75566..75772
                     /locus_tag="OO145_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465348.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            76084..76569
                     /locus_tag="OO145_000079"
     CDS             76084..76569
                     /locus_tag="OO145_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5465349.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            76550..76828
                     /locus_tag="OO145_000080"
     CDS             76550..76828
                     /locus_tag="OO145_000080"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465350.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            76821..78281
                     /locus_tag="OO145_000081"
     CDS             76821..78281
                     /locus_tag="OO145_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5465351.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            78315..79125
                     /gene="murI"
                     /locus_tag="OO145_000082"
                     /pseudo
     CDS             78315..79125
                     /gene="murI"
                     /locus_tag="OO145_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
     gene            complement(79244..79938)
                     /locus_tag="OO145_000083"
     CDS             complement(join(79244..79692,79692..79938))
                     /locus_tag="OO145_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5465352.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(80457..81830)
                     /gene="eutA"
                     /locus_tag="OO145_000084"
     CDS             complement(80457..81830)
                     /gene="eutA"
                     /locus_tag="OO145_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5465353.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(81805..82284)
                     /gene="eutP"
                     /locus_tag="OO145_000085"
     CDS             complement(81805..82284)
                     /gene="eutP"
                     /locus_tag="OO145_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5465354.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(82297..82632)
                     /gene="eutS"
                     /locus_tag="OO145_000086"
     CDS             complement(82297..82632)
                     /gene="eutS"
                     /locus_tag="OO145_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5465355.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(82925..85204)
                     /gene="maeB"
                     /locus_tag="OO145_000087"
     CDS             complement(82925..85204)
                     /gene="maeB"
                     /locus_tag="OO145_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5465356.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            85493..86443
                     /gene="tal"
                     /locus_tag="OO145_000088"
     CDS             85493..86443
                     /gene="tal"
                     /locus_tag="OO145_000088"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5465357.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            86463..88466
                     /gene="tkt"
                     /locus_tag="OO145_000089"
     CDS             86463..88466
                     /gene="tkt"
                     /locus_tag="OO145_000089"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5465358.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(88569..89612)
                     /locus_tag="OO145_000090"
     CDS             complement(88569..89612)
                     /locus_tag="OO145_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5465359.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(89738..90313)
                     /gene="nudK"
                     /locus_tag="OO145_000091"
     CDS             complement(89738..90313)
                     /gene="nudK"
                     /locus_tag="OO145_000091"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5465360.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(90381..92360)
                     /gene="aegA"
                     /locus_tag="OO145_000092"
     CDS             complement(90381..92360)
                     /gene="aegA"
                     /locus_tag="OO145_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5465361.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            92566..94283
                     /gene="narQ"
                     /locus_tag="OO145_000093"
                     /pseudo
     CDS             92566..94283
                     /gene="narQ"
                     /locus_tag="OO145_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            94485..97598
                     /gene="acrD"
                     /locus_tag="OO145_000094"
     CDS             94485..97598
                     /gene="acrD"
                     /locus_tag="OO145_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5465362.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(97697..97756)
                     /gene="ypfM"
                     /locus_tag="OO145_000095"
     CDS             complement(97697..97756)
                     /gene="ypfM"
                     /locus_tag="OO145_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5465363.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            98137..98493
                     /locus_tag="OO145_000096"
     CDS             98137..98493
                     /locus_tag="OO145_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5465364.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            98497..99624
                     /gene="dapE"
                     /locus_tag="OO145_000097"
     CDS             98497..99624
                     /gene="dapE"
                     /locus_tag="OO145_000097"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5465365.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            99652..99852
                     /locus_tag="OO145_000098"
     CDS             99652..99852
                     /locus_tag="OO145_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5465366.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(99962..100660)
                     /gene="ypfH"
                     /locus_tag="OO145_000099"
     CDS             complement(99962..100660)
                     /gene="ypfH"
                     /locus_tag="OO145_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5465367.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(100734..102749)
                     /gene="tmcA"
                     /locus_tag="OO145_000100"
     CDS             complement(100734..102749)
                     /gene="tmcA"
                     /locus_tag="OO145_000100"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5465368.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(102764..103627)
                     /locus_tag="OO145_000101"
     CDS             complement(102764..103627)
                     /locus_tag="OO145_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5465369.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(103795..104508)
                     /gene="purC"
                     /locus_tag="OO145_000102"
     CDS             complement(103795..104508)
                     /gene="purC"
                     /locus_tag="OO145_000102"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5465370.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(104721..105755)
                     /gene="bamC"
                     /locus_tag="OO145_000103"
     CDS             complement(104721..105755)
                     /gene="bamC"
                     /locus_tag="OO145_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5465371.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIEPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(105772..106650)
                     /gene="dapA"
                     /locus_tag="OO145_000104"
     CDS             complement(105772..106650)
                     /gene="dapA"
                     /locus_tag="OO145_000104"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5465372.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            106796..107368
                     /locus_tag="OO145_000105"
     CDS             106796..107368
                     /locus_tag="OO145_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5465373.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            107368..107838
                     /gene="bcp"
                     /locus_tag="OO145_000106"
     CDS             107368..107838
                     /gene="bcp"
                     /locus_tag="OO145_000106"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5465374.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            108091..108708
                     /gene="hyfA"
                     /locus_tag="OO145_000107"
     CDS             108091..108708
                     /gene="hyfA"
                     /locus_tag="OO145_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5465375.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            108708..>109692
                     /locus_tag="OO145_000108"
     CDS             108708..>109692
                     /locus_tag="OO145_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465376.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVAHFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFARFHFLRGWRRRCFCWPFSVL
                     ARKRQPRFCQFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPSHASA
                     LMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALA"
BASE COUNT        27803 a        28157 c        26499 g        27233 t
ORIGIN      
        1 cagccagatc tcgtccagca tcacgccaag cctgctcaaa tccctgccga cgataccctg
       61 ttgccaacgc actttccggt agccagaagc ataatggttg acgatagccc gccgcgagca
      121 aatgctgtgt tgattcatat tgtgcagtgt aatcatcagg gatataactg ggtaacgctg
      181 ggtcatccgc cacacagttc gccaatacaa tattttcacc atacagagac tcaggcagcg
      241 tgatatgtcg cagccccatt gtagtataga taatgccatc cggacggtgg gcaagcagct
      301 gacgtgccgc gcgggcagcg tcatcttcag aaaaaatatt gattaaaaaa ctattccagc
      361 cgaactcgct ggcggtttgc tcaatggcaa gcagaatatc aacagagaaa ggagtggtag
      421 ccgtgtcctg cgccagcacg gcgagagtcg acggcttacg tccttgagcg cgcatcttac
      481 gggcggaaag atcaggaaca taattcaggg tctggattgc ctgcaatacg cggtcacgcg
      541 ttgcaggacg cacagattct gcattatgca tcacccggga gactgtcatc atcgacactc
      601 ccgccaggcg tgcgacatcc tttaatgaag ccatacccaa gccgtttgcc gtaaaacggg
      661 cactgtagca gaaacagacg tcactggcga gatccaacgc cctatcacct gacacagcaa
      721 tacaataaaa aataacaata attcccggac aattgtcccc aattccgcct ctgttctcgc
      781 caacgagtaa ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag
      841 aagaaaatat aaatatggat cattgtgctg ccactgtatc ccgacatcag cccggaaatt
      901 attgcgattg ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc
      961 tggctggtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
     1021 gcgacattta tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc
     1081 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc
     1141 cgttggtaaa ggatctggtt gctcttaaag aaaccacctg gtttaatcct agcacgacct
     1201 cattggctga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
     1261 cgcgcttatc ccgttttgca ccctatctgg caaaagcatt tgctgaaaca gctgccactg
     1321 ggaggattat tgaatcagaa ctggttgcca ttccggctat gcaaaaacgg ctggaaaagg
     1381 aatatcaaca accgatcagc gggcaactcc tactgaaaaa agatagccat ttgcccattt
     1441 ccggctctat aaaagcacgc ggcgggattt atgaagtcct ggcacatgca gaaaaactgg
     1501 ctctggaagc ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt
     1561 ttaaacagtt ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat
     1621 caatcggcat tatgagcgcc cgcattggct ttaaggtgac agttcatatg tctgctgatg
     1681 cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc
     1741 aagattatgg tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
     1801 tctttattga tgacgaaaat tcccgcacgt tgttccttgg gtattccgtt gctggccagc
     1861 gtcttaaagc gcaatttgcc cagcaaggcc gtatcgtcga tgctgataac cctctgtttg
     1921 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcggg cttaaactgg
     1981 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt
     2041 taggcgtcca tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc
     2101 ttaccgcagc ggatggcctt gcagttggtc gcgcatcatg ctttgtcggg cgggcaatgg
     2161 agcgtctgct ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct
     2221 ggctggcgca ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac
     2281 ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat
     2341 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
     2401 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcaacatcg caatcctttc
     2461 cctgggtgag cgatgctgcc gatggcgcag acttaagatc cccggtctta cccgctataa
     2521 ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
     2581 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
     2641 tagaaagtga ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac
     2701 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
     2761 ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg
     2821 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
     2881 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
     2941 cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
     3001 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactgaat gtcactaaca
     3061 cccgcatgtc tattttattg ccataacgtc ctatcaaagg tgaaatcaat agtggcatga
     3121 tgccgatggg cgcataagca agcccggccc atatcgcatt ataccccatc gttttctgga
     3181 gtaactgcgg cataaggacg atcgctccag agtaaaataa atacgcgcag gtgatactca
     3241 caataccaat ggtgaaatta cgggacttaa acaaactgag atcaagaatc gggttctctg
     3301 aggtcgattc ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta
     3361 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
     3421 caccaacacc gagcactaac agggtcagtc ttggtagatt catttttacc ggtgaagttt
     3481 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
     3541 cattgattaa aaatatccaa ccccagctaa agttatcgca aatatagccg cccaatatcg
     3601 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
     3661 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
     3721 ttaatccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca
     3781 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc
     3841 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
     3901 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
     3961 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa
     4021 cgcaccataa cgtcccaccg gttaatggtg ccggagttga tttagtgatt gccattaaag
     4081 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
     4141 taaagcctta ctggtataag caggcatgga ggtcacggtt gaagccagat caggcatctc
     4201 agcaatgtct tcgtccttcg tatcaatagt tgcagtcatc gataaaccaa tgcgcaaggg
     4261 gtgttccatg agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt
     4321 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
     4381 attgatccct gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat
     4441 attgactgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
     4501 ccacatttga cgtgccggta cgacagccat taacgattgt ccggaactca ctgtttcgcc
     4561 gacctgaaca cttcgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt
     4621 aagcaccaac caggcttctt tggttgcatc cgccgcttca actacttgtg gctgacggtt
     4681 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag
     4741 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
     4801 cccctgcttc gctaaaggca cacgacggtt ataatcttct aaagattgtt gatactgaat
     4861 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
     4921 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
     4981 atccagtgaa actaaaatgt caccctgtcg aacgtagttc gtatctttat gattaactac
     5041 agtgacacta cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
     5101 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
     5161 aataaataaa actatcacca ataaagaaaa gtattttttt ctgttagact gttttttatt
     5221 tgaattaatc tgttccacta ttatctctca tttcgcatag atgaaattta tgaattgaat
     5281 gaaaagagta ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa
     5341 cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt
     5401 ttgcaataca attcttacgc ctgtaggatt agtgagaaga cttatagtgc taacttgaaa
     5461 ctataaatca tcggtataat ccctgatttt attgttgaca tttcatttat gccgactatt
     5521 tatatggtat acttgtcgaa ttatcttaaa ggaaactcag attttcttat ttttattgag
     5581 aaaatgagat gacgccttat gtctgtatta ctacagggag aagggagatg cttcattgca
     5641 aagggaataa tctatgaacg caataattat tgatgaccat cctcttgcta tcgcagcaat
     5701 tcgtaattta ttgatcaaaa acgatattga aatcttagcg gagttaactg aaggcggaag
     5761 cgccgttcag cgggtggaaa cacttaagcc tgatatcgtc atcattgatg tcgatatccc
     5821 cggagttaac ggtatccagg tgttagaaac gctgaggaag cgccaatata gcggaattat
     5881 tattatcgtc tccgctaaaa atgaccattt ttacgggaaa cattgtgctg atgctggcgc
     5941 taatggtttc gtgagtaaaa aagaaggcat gaacaatatc attgcggcta ttgaagctgc
     6001 aaaaaatggc tactgctatt tccccttctc tctcaaccgg tttgttggaa gtttaacgtc
     6061 cgaccagcaa aaactcgact ccttatcgaa acaagaaatt agtgtcatgc ggtatattct
     6121 tgatggcaag gataataatg acattgcgga aaaaatgttc atcagcaaca aaactgtcag
     6181 cacgtataaa agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg atctttacac
     6241 attcgcgcaa cgtaacaaaa tcggctaatc acatgaagtt tttaccctat ttttttcttc
     6301 tctgttgtgg tctttggtcg accataagtt tcgcagacga agattacatc gaatatcgtg
     6361 gcatcagtag taacaaccgt gtcacacttg atccagtacg tctgagcaac aaggaattac
     6421 gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa acggctacct
     6481 tgttgcatac cgattcgcag caacaggttc gtggtattaa tgctgattat ttaaatcttt
     6541 taaaaagagc gttaaatatc aaattaacac tccgggaata cgcagatcat caaaaagcaa
     6601 tggacgcgct tgcagaaggt gaagtcgata tagtgttatc acatttagtt acttcgccgc
     6661 ctcttaataa tgacattgct gcaaccaaac cattgataat tacctttccg gcgctggtaa
     6721 ccacccttca cgactcaatg cgaccgctta cctcaccaaa accagtaaat attgctcggg
     6781 tagcaaatta ccccccagac gaggtaattc atcaatcatt tccaaaagca acaattatct
     6841 cttttacaaa tttatatcag gcattagcat ccgtctcagc tgggcacaat gattacttta
     6901 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
     6961 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatctg
     7021 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
     7081 aagtatcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
     7141 tcactgaaca tgaaaaacag tggattaagc agcatcccaa tttaaaggtg ctggaaaatc
     7201 cttactcgcc cccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
     7261 acattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
     7321 acaatatcca tgctggaaca caacttagcc ccggaggatg ggatataata cctggcgcta
     7381 tttatagtga agatcgagaa aataatgttt tatttgctga agccttcata acaacgcctt
     7441 acgtttttgt catgcaaaaa gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag
     7501 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
     7561 aatggataca ggtcgataat gccagcgctg catttcacaa gattaaggaa ggtgaacttg
     7621 atgctctggt cgcgacacag ctaaattcgc gttacatgat cgatcattac tatcctaatg
     7681 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
     7741 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
     7801 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
     7861 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
     7921 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
     7981 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
     8041 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
     8101 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgattca ccctttaaag
     8161 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
     8221 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
     8281 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
     8341 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
     8401 tagcaaaaag tcagtttcta gcaacgatga gtcacgaaat aagaacacca ataagctcta
     8461 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
     8521 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
     8581 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
     8641 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat
     8701 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgaccct caggcgttta
     8761 agcaggtctt atcaaattta ctgagtaatg ctctcaaatt taccaccgag ggggcagtaa
     8821 aaattacgac ctccctgggt cacattgatg acaaccacgc tgttatcaaa atgacgatta
     8881 tggattctgg aagtggatta tcgcaggaag aacaacaaca actgtttaag cgctacagcc
     8941 aaacaagtgc aggtcgtcag caaacaggtt ctggtttagg cttaatgatc tgcaaagaat
     9001 taattaaaaa tatgcagggc gatttatcat tagaaagtca tccaggcata ggaacaacat
     9061 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
     9121 aacaacccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
     9181 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
     9241 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
     9301 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
     9361 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
     9421 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
     9481 taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
     9541 ccctgaaaaa taatacggcg aatgatctac aactgatgca ggagattctc atgactttcc
     9601 agcatgaaac gcataaagat ctacccgctg cgtttcatgc actagaagct ggcgataaca
     9661 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
     9721 agttgattaa tattagccat cagttagaaa taacacctgc ttcagatgac agtaagcctg
     9781 aaattcttca gctgctgaac tctgttaaag aacacattgc agagctggac caggagattg
     9841 ctgttttctg tcagaaaaat gactaaattg cggctcccac aatgttctaa tgtgggagct
     9901 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
     9961 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
    10021 attacccggc atcattattc ctccggcttc aatcaacata tttctcgcct gagtttgtgg
    10081 caaattaatg gcctcagcca cacttaataa cggcgcgacg ggcacaccaa cttcatgtat
    10141 tttggctaac caaacttcag ctgcctgcgt ttttaacgtt cgctcaatat attgtttaag
    10201 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
    10261 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
    10321 aatcggctta tcctgagtat cgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
    10381 caggcgttgt ggtgacttcc ctgtcgcaat atatgccatc agaccatgct ccagaaaact
    10441 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
    10501 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
    10561 ggtaccaaca cgcactggcg gagcatcagg gtaccctgtt tccatcatta tcccactcat
    10621 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
    10681 tgacgatgaa gcatatatga ggcgcgggtt gatttcttgt aacctttccc atgaaaaccc
    10741 cagtttttcc attgtaccta ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
    10801 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
    10861 atgattaata aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt
    10921 atcatcacca tgacctggtg gctcaacttt aattaccctt gcgcccatat tataaagaag
    10981 ttgagttccg aaaggtccat taaggacatg tgtcatatcg ataactaata agccttcaaa
    11041 cggccctttg ctttcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa
    11101 agacacttcg gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga
    11161 cataaatcca caagggagcc gtgacgacaa agcccagtac gctcacagcc aatgacgctg
    11221 taccagtacg ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg
    11281 gtaatgcgcc tgccagtacc atcatctgca aatgttcgct gttcaaatga catgccatac
    11341 caacgagaag cagcgccagt ggcatcagaa tcaacttcag gaaggtgtta taagcaattt
    11401 cggcactgaa ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc
    11461 ctgagttagc cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc
    11521 caaccagcac caggatcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag
    11581 aaattaatgc gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca
    11641 aatacagacc aataggaata gtaattgcgt taacaataat agaaataatt gctaccacta
    11701 aacctgttga tacggaatca ccataaatag gatcgagaac tgcaaacccc aggaatccaa
    11761 tggtaggtga acccgcaatt aatgcacata cagctgcttc tgcatgggta cgtttaaaaa
    11821 atttgtagca accaaaccag gagaaaaaga aacatccgac aataaccaca agtgatacaa
    11881 gggtcagacg agtgtccgca aaaatcattt ccctgtttgc ccgggtaata gaaacaaata
    11941 gagccgcagg aagagcgtag tttaatacca gtttattaaa tgcgcgggct tgatcttctg
    12001 aaaaagtttc tcgtctgccg ctaaaataac ccaacagcat gattacgata ataggcaata
    12061 aatcgccaat aaaaaatgtt agcataatag tatttccgat aagggtcccc tcaccatcgc
    12121 ataatgagtt ggcaagagga gacgatgtca gtatttaatt accagcgact tgttttgggt
    12181 taagtttggt aatatggcca ctttcagttc ctgctgcagg gtcgataacc acattaataa
    12241 tggtcggttt gcgcgactga ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg
    12301 tgacgttata gccaacgcca cgaaacgcat ccattaattt gtcatacctt gcatggtgca
    12361 acagatccgt tggtgatggt gcaccagcgc cactgagatc aacaccgtct cctctgtaga
    12421 tgccgccatt attaaaaata acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa
    12481 tttccatccc actgaaacca aaagcactat caccttcaat ggcgacaacc ggagaaccag
    12541 aagtcacgct agcaccgatg gcatagccca taccgatgcc catgacaccc caggtaccac
    12601 aatccagacg acgacgtggt ttatacatat caataatatt tcgtgcatta tccagggtat
    12661 ttgcaccttc attaactaaa taaatatcct ggttctcgcg caatacgtcg cgcacagcac
    12721 ttaatgcatt aaagtaattt aatggttgag tatctgtact taatttttca tgcatttttt
    12781 gtgcattttg ctgcttgtgg atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg
    12841 tgttttgttt cagttctgcc agcatacctt gcatactgga tgcaatatca ccaacgactg
    12901 gcacagcaat ggggcggttg ctgtcaattt cctgcggttc aatatccagt tgaataaact
    12961 gtgtatctgc cgcccatcct tttttaccgt gtgccaataa ccaattcagt cgtgcaccaa
    13021 caagcatgac aacgtcagca tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg
    13081 gatgcgtatc ttcaaggatc cctttcgcca tagacattgg caggaatgga atctgagcac
    13141 tttcaataaa ttcacgaagc tgttcatcag cttgtgaata cgccgcgcct ttgccaagga
    13201 taattaatgg ccgttcagct tttgctaaaa gcgaaattgc gctagtgact gacttcgggc
    13261 atggcaataa tgctggcgac ggattttcaa ctttaacaat cgtggttaac gcttcgtctt
    13321 tttccatcgt cgcggccagg acatttgctg gcaaatcaag ataaactccg ccagggcgac
    13381 ccgatacaga gacccggata gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa
    13441 cgcgaaatgc tgctttggca tacggttttg ccgcattcat ttggtccagc tcttcataat
    13501 caccttgctg taggtcgacg atcgcgcggt cgctggagcc gctaatcatg ataatcggaa
    13561 aaccatttac cgttgcgttg gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa
    13621 ctgtcaggca gatccccggt ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg
    13681 ccgactgctc atgacgaaaa ccaatataac gaatgccttc cgcctgggca tggcgtgcca
    13741 tatccgtcac aggaatacct acaacaccat aaatagtgtc aatattattc tgttttaatg
    13801 cttcaacgat gatatgcata ccatctgtca tttgaagttg atctgacatg attaataacc
    13861 tcgaaatttt tataaattgg gagccagaag cccccgttaa tatcagatgg cgtggttttg
    13921 cttcattgca gcaatttcat cgtcgctata acccagctcc tgcaatacag cagcggtatg
    13981 ttcacctaat agcggcgcag ctttaatatc cggcgtaaag gcagagaatt tcattggaca
    14041 accaacggtc agatattttc cacgcaacgg ttgttccact tcaacaacac tgccactttg
    14101 gcgcaaagag ggatcaagtg aaatttcttt catacttaaa accggtgcac aaggaatatc
    14161 aaactgagtc aaataggcca ccgcttcatg tttatcaata gtgacagtgt atttttcgat
    14221 ttcagcaaaa atatcgaaaa tatgtggctg tcgggcatgg gctgtactgt atgccggatc
    14281 ggtaatccat tctggtttgc cgatggcttt acaggtgttt tcccagtttt gctcctgaat
    14341 agtgaaataa atataggcgt taggatcggt ttcccagcct ttacatttca ggatccagcc
    14401 aggctgaccg ccaccacctg cattaccacc gcggggaact gcatcaccaa atgtaccatt
    14461 cggatactgc gggtattctt ccagataacc caatttatcg agacgctgct ggtcacgtaa
    14521 tttcacgcgg caaaggttca atacggcatc ctgcattgac atggtgactc gttgcccacg
    14581 ccccgttttt tcgcgatgca gcaaagcagc aagtaaaccg atcagcaaat gcattccggt
    14641 gttgctgtca cccaacgctg cagcgcttac cagcggcgga ccatcccaaa aacccgtagt
    14701 ggatgccgcg ccacccgctg cctgagcaac gttttcatag gcttttacat tcacataagg
    14761 cgaacactca tcaaaccctt tgatcgaacc aaaaatcaga cgtggattga tttcttgaat
    14821 atgctcccag gtgaagccca tgtgatcaat ggcccctgga tgaaagttct cgactaagat
    14881 atcagcttcg cggatcagct tttccattac ctctttgcct tccgctgttt tggtatttaa
    14941 ctcaatagaa cgtttgttac tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat
    15001 atctcgcagt tggtgacgcg ttacgtcacc aacgccggga cgttcaattt taataacgtc
    15061 agcgccaaac caggccagca tttgagtaca agatgggcca gattgcacac cggtgaaatc
    15121 gagaacttta attccttgaa gtggagttga catagcaata cctattagtt aattaaatat
    15181 gtgttaattt agcgtttatt taaggcataa cgccctctat aaataatctg caaaatatct
    15241 tcgtagcggc actcgatatt tttaaattcc tgtatcaagt actgaaaatt attttatcgc
    15301 cacttccaat tcctttctgc atggtaagta gtgaggacga aaagaagaat atgataaata
    15361 ttaattcgtg gtaatcagag agagtcgcga catttgtgat gttattctta caactgtaag
    15421 aagcgccggt gatttacaag taagagtatg attacgcttt ttaaagcgta ccataaagca
    15481 taacataata aatgatttgt gatgacacgg gattattgcg gtttactttg aggaaaataa
    15541 aatgcgtgtt tatagaaccg cgagcgccaa ataattttat gaccacaacc tgtcgtattg
    15601 tggtcataaa aaattgtcgg ctggagggag ttaaatcctt tacctcaata tactgcctgt
    15661 cagcattagt tcacaaacag tgcttcgcta tcgacgatga ttccatcttc cgcaccttta
    15721 agcgccaggt ttgcctcgta gtattgcttt ttatcaagca gtttctgcgc atcagcaagt
    15781 gcattacgag tctgtttaag cggcatcaga tattggtttt ccattacgcc aacaccggcc
    15841 aggcgaagct cttccattgc gcccttttta tcccctttcg ccattttttc attggcaatt
    15901 tttatcgcgg cttctttttc gggtgttgct acataactct cagaaattcc gacagatgca
    15961 ttaatgacaa tatattggtc atcattgaga ttggtctttt tacctggctt agcgaacttt
    16021 gcccattcgg tactgtcatc tgacagcaaa gcggaagctt cattggtcag ttcttttgcc
    16081 ttttccggat cgccatgaaa cagagccagg cgcgctacct gcacgtcacg catcgcatat
    16141 aaaccttgtt cagagatacg ctctgctgct gccgttttct gggtttgctg agtttgctgt
    16201 tgtgccgcta ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg
    16261 accatcgtag ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa
    16321 cgccgccatc atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa
    16381 agggttatct tacaattgta gttattatcg caaagcggca tttagtctta cagttgtagg
    16441 aatatgcctc ctgtcgcaga gaactattct tatataccac aaagagatgt catttattat
    16501 attgatgtaa atattgatta attatatgta aatacagcca taacatcgta tggttctcag
    16561 tgaaccattt tcaggataac tttctatgaa agtaaactta atacttttca gcttattttt
    16621 attggtctct attatggcat gcaatgtttt tgcattttcc atttctggtg gtgtaagtga
    16681 gaggagctat aaagagactg aaaaaacatc agcgatgacg accacacact ctacaaaact
    16741 tcagccatca caggcgattt tgtttaagat gagagaagat gtgccaccat taaacctcac
    16801 agaagaaatc acgcctactt atccgacaaa ggcgaattat cttattcatc ctgtgcgata
    16861 gcgtctgtca catggcaggt gaacaatcat gtctgatgct gatgtgaaca cctgcccaaa
    16921 tgagccatcc tgacccacta tcgcctgcca taccccacca taaagcgaat catcaagcca
    16981 caagccgggg caagaaacat aaatatcccc gtcaccagtt cattaccgcc agttaaccac
    17041 atggtgaagt aataaatgag tgcagcgagg atcaccatgc cgaagaaaga agagaccgcc
    17101 gataccacgt tcagtacctg accgatgtag ccgcttacgc agacaataca cagggcgaga
    17161 aacatccata aataaatcat agctcaacct cctgccgcgc taaaaaacct gtaacttcag
    17221 tcactttaat agtaagacaa tccttaacct ctggcaattt ttgctaagca tcaatgaaaa
    17281 cagcacgtta aatgcaacgc tattgcgtgc ggaaaatttt ttactttgca cgattaataa
    17341 tcagtagctg aaagcagtca gccagtgata aagtagagcc accgcattca tgctcctcgt
    17401 ggttatgtcc tgactagtct ttacactctt tacaggaacc attgtcgtac atgatggccc
    17461 aaccaattga gtgtaccgct gtcgacatgg aatcttctgc gatacaacaa ttcgtatcta
    17521 cagaaggtaa ctatgtttcc acaatgcaaa ttttcccgcg agtttctaca tcctcgctac
    17581 tggctcacat ggtttgggct tggtgtactc tggctttggg tacagcttcc ttatcctgtt
    17641 ctctgctttc tcggcacgcg tattggcgca atggcgcgac cattcctgaa acgtcgtgaa
    17701 tctatcgccc gtaaaaacct ggaactttgt ttcccgcagc attctgcgga agaacgcgag
    17761 aagatgattg ccgaaaactt tcgttcactc ggcatggcgc tggtagaaac cggcatggca
    17821 tggttctggc ccgacagtcg cgtacgtaaa tggtttgatg ttgaagggtt ggataacctt
    17881 aaacgcgcac aaatgcaaaa tcgcggcgta atggttgtcg gcgtccattt tatgtcgctg
    17941 gaactgggcg gccgcgtgat gggactgtgc caaccaatga tggctaccta tcgtccacat
    18001 aataatcagc tgatggaatg ggtgcagacc cgtgggcgca tgcgctctaa caaagcgatg
    18061 atcggcagaa ataatctgcg cggcattgtc ggtgcactga agaaaggtga agcggtatgg
    18121 tttgctcccg atcaggatta tggtcgtaaa ggcagctcct tcgcgccgtt ctttgcggtg
    18181 gaaaatgtcg ccacaaccaa tggcacctat gttctctccc gtctctctgg cgcagccatg
    18241 ttgaccgtaa cgatggtaag aaaagcggat tacagcggat atcgtttgtt catcacccca
    18301 gagatggaag gctacccgac agatgaaaat caagccgctg cttatatgaa caagattatc
    18361 gagaaagaga tcatgcgcgc accggagcag tacctctgga tccaccgtcg ctttaaaacg
    18421 cgcccggtgg gagaatcgtc gttgtacatt taacaaactg ctcttactct ttccctcctc
    18481 aaatcggcca taataacctc atgattttta tagctttttt cttaagtttt ttccttcgct
    18541 gaatatctaa ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt
    18601 actaaaagta aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc
    18661 cgtctcgagc agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt
    18721 taaggtcgct ttgcccggtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt
    18781 aacagtaatt acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg
    18841 gtcatcgttt gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa
    18901 acaggagcca tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt
    18961 gaatatgttt gctgctgacg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc
    19021 tgatggcctg acgaatacgg tcgtggtttt caatcagtgc aaagcgaaca tgcgtatcac
    19081 catagtcgcc aaagccaatc cccggcgaga cacaaacctt cgcttcgtta aggagcttct
    19141 tggcaaattc cagcgatccc atggccgcat atggttccgg gattttcgcc cagacataca
    19201 tcgaagcctt cggcatttcg accatccagc ccgcttcatg cagcccttta accaatacat
    19261 cacgacggcg tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctcgagtg
    19321 ccgcaatcgc cgccacctgc aacggcgtaa aagtgccgta atcgtgatag cttttgattc
    19381 gcgccagggc actgaccagc gttttgttgc cgaccataaa gccgatacgc cagcccgcca
    19441 tgttgtagct tttcgacagc gtaaagaact cgaccgccac atcgcgtgca cccggcacct
    19501 gcataattga cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcatgga
    19561 ccaccagcac atcgtagcat ttcgccagcg ccacaacctt ttcgaagaac tccagttcca
    19621 cgcattgcgc ggttgggttc gacgggaagc cgaggatcat catcttcggt ttcggataac
    19681 tttcacgaat ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg
    19741 agcgtacctg cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg
    19801 gcaccagcac cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt
    19861 tcgaaccaat agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct
    19921 gataccagcg ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat
    19981 gcgtgtccgg gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac
    20041 cgtccgggtt acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt
    20101 tcagttcagc ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac
    20161 gttcagggcg agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga
    20221 gcgacgctgc cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag
    20281 cgttttagaa catttttatc aatggattta aatgaggaac tccacttcaa aaacaggaaa
    20341 tttttctacg ctaacaagaa aatcaccacc aattttatta acattataat ttcaataaat
    20401 ttaccagcca gaagtgcatt atcaagagat acattgctaa tacaccattt atcttttaac
    20461 ccccttttaa atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt
    20521 acctatcata gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc
    20581 tgctggcggt cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta
    20641 tccgcctgtt tcgcgaactg ttgcacaagt cggcgcactc cccaaaagaa ttgctcgccg
    20701 ttaccgccat tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag
    20761 gctcgctggt gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt
    20821 gggtaggcat cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg
    20881 gcgtgacggc gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga
    20941 taaacctgaa aatccccaaa gcacagcgct ggcgctggat atcgccaaca aaacgctacc
    21001 gctgtttcgc catgtcaata gcgagtcatt acgcaaggtc tgcgaaatta tccgcgatga
    21061 cattcacgcc gatgcggtgg cgattactaa taccgatcat gtgctggcct atgttggcgt
    21121 gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc gtcaggcgat
    21181 gaattacgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac cagagattca
    21241 ttccatgctg gtgatcccat tgtgggagaa aggggtcgtg accggaacgc tgaaaattta
    21301 ctactgccac gcgcatcaga tcacctcgtc attacaggaa atggcggtcg gtctgtcgca
    21361 aatcatctcc acgcaactgg aggtttcacg cgccgagcag ctacgtgaaa tggcaaataa
    21421 ggcagagatt cgcgccctgc aaagcaaaat taatccccat tttctgttta acgctctgaa
    21481 cgctatttca tcgtcaatcc gtctgaatcc ggataccgct cgccagttga tctttaatct
    21541 gtcgcgttat ctgcgctata acattgaatt aaaagacgat gagcaaatcg atatcaaaaa
    21601 agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg gcgacaagct
    21661 gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc tgctgatcca
    21721 gccgttggtg gagaacgcca ttgtccacgg tattcagcct tgcaaaggta aaggcgttgt
    21781 caccatcagc gttgcagagt gcggaaatcg ggtacgcatt gcggtgcgag ataccgggca
    21841 cggcatcgtt ccaaaggtga ttgagcgggt cgaagcgaat gaaatgccgg gcaataaaat
    21901 tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg
    21961 ccgcctggag ccggggacgg aaattgcgtt ttacattcct aaccaacgca ccccagtcgc
    22021 ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat cattgttgaa
    22081 gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag ccagatggag
    22141 attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca taaccgcgtc
    22201 gacgccattt ttctggatat caatattccg tcgctggatg gcgtgttgct ggcgcaaaac
    22261 atcagccagt tcgcccataa accgtttatt gtgttcatca ccgcgtggaa agaacatgcg
    22321 gtagaagcgt ttgaactgga ggcgtttgac tacattctca aaccgtatca ggagtcacgt
    22381 attaccggga tgctgcaaaa actggaagcg gcctggcaac aacagcagac cagcagtacg
    22441 cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaata
    22501 atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat gacctttgtc
    22561 tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgaattttg cagcaaactg
    22621 ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatacgc
    22681 gaaatcgaac cgtggtttaa taacacctac attctgcgac tgaaagatct ggattttgaa
    22741 gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt taatgcatct ttaatcggga
    22801 actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc
    22861 ggcctagtgc ttaacccgca actactgggg cgcgtctggt ttgccagcca gcctgcctcg
    22921 ttaccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct gcgcggcgaa
    22981 tacggcaacc tgctggaagc aaagcagcag cgaatggtgg aaggagaaat gctgtttatt
    23041 ccggcacgcg cggctaattt accgatcaac aacaaaccgg tgatgctgtt aagcctggtg
    23101 ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc gttgttgcat
    23161 cctgctcgcc agacccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc
    23221 gccctcaccc atcttagccg ttcgccgctg gagcagaata tcattcagcc actggtgtta
    23281 agtttgctgc atctttgccg caacgtggtg aatatgccgc cgggtaattc acaaccgcgc
    23341 ggcgattttc tctatcacag catttgtaac tgggtttagg ataattatgc ccagccgctc
    23401 acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt
    23461 gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat ggcgaaggcg
    23521 aggatgattt tgcagaaata tcatctgtca attcatgaag tggcacagcg ttgcggtttt
    23581 ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttagtctgac gccgggagag
    23641 tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag cagcgtcagg
    23701 atactttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt caccagttta
    23761 cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcacc cagcgtcagc
    23821 atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt cgccagccgg
    23881 gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat cgctacgcca
    23941 aaaccaacgc cggtggtaac aatctcttcc cgctgccaga catcttcttc cagctcaaac
    24001 ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca ggaactggat cgcctgctct
    24061 ttattgctaa aatcctgatc aacaaagata ttctccagcg ccagcagtgg gcgaacgtct
    24121 tcttccggcg taaaggcggt gagtaacgct tcaatttcct gcgcactgcg gcattcacat
    24181 gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcacc
    24241 gccggaatac gcgggctact catactcagc tcatccaggc ccagcccaag cagtagcggc
    24301 agataacggc tttcaccgcc cagttcaccg caaatgccta cccatttgcc ccgctgatgc
    24361 gcagtggtaa ctatttgctg caacatgcgc aggaacgatg gcgtaatcgg gttatatagc
    24421 ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat atcgttggag
    24481 ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca caccgacggc
    24541 acttccacca tgatcccaag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc
    24601 tcaacgatcg ctttttggat ctcgcctttc acccataaga tctgatcgag gctgtgaacc
    24661 atcgggatca tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc ccgcagttga
    24721 gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg
    24781 ttctcttcct ggggaatatt cagataagga atgcttttat cgccgccgat gtccatcgtg
    24841 cggaagataa tcggcttgtc gcccgccgcc agtagcacct gctggtaggc ttcaaattgc
    24901 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg gaacagcccg
    24961 acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc gatattcgcc
    25021 gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc ctgcgcctgt
    25081 tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca
    25141 ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa
    25201 cgggcaatcg catccagcgg caagccactc agtaccggga tcgccgaggc acgggccaga
    25261 atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag atttttcaaa
    25321 tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttcc
    25381 agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa ctgttcgctg
    25441 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agatagtttg
    25501 gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg ctgttctgtc
    25561 atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgagcgctg
    25621 aggatagttt tgctttcgcc gtcacgctca cgcaattgct gattcagttg ctcggcaagc
    25681 gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat tgcccgataa
    25741 ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc gcttgccagc
    25801 acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag cggatgcgcc
    25861 gtcagttctg cctgcgtcgg ctgaacgctg tcgctgtcga taaagcgcac caggatgtac
    25921 tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag gctgcaactg
    25981 tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc agcctttgcg
    26041 ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg ttctttaagt
    26101 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
    26161 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
    26221 cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcgcggcaa
    26281 tcggcaatcg atgccgcgca atgtccgtgg cgggtggcag gccccattac caatgtgggt
    26341 acgccagtac cggttaagtg caccgctcca ccgtccgtgc cgccgttact gaacatatcc
    26401 gcctgcaacg gaacgccaat ttctgccgcc acggtttcaa tccacgcggt aagttttggc
    26461 ggcgcaatca gcgatttatc gcttaacacc agcatcggcc cgttaccaat ctgacgatgg
    26521 ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac aatggcgacg
    26581 tccggcgaca ccgcgcgggt agcagtttgc ccgccgcgta atcccacctc ttcgctggaa
    26641 cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg tagtaacgtc
    26701 accagcagat agcaactgag gcgatcatca aaggctttgc ccatcacccg ctggtgaggg
    26761 aggacctgaa aagtggtatc gaaggtgacg cgatcgcctg gacgaattcc cgcctgcatc
    26821 acttcgtcat aggtgcgcgc gccaatgtcc acgcgcatgg cgctaacgtc attcccctgc
    26881 cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtggtgat gcgcaccggc
    26941 tgcagctggc gggcagccat gcgcacgttg ccaaccggca gcacgtcaat agcgccttcg
    27001 cgggagatgc tgcgcaccat aaatcccact tcgtccatat gggcgcagat catcaccttc
    27061 ggacctgtcg attcattgag gcggatcagc accgatccca gaccatcaaa tcgcacttct
    27121 ttttgcaggc gagccgcttc ttccagcagg atctgccgca cttcctgttc cgaggaggcg
    27181 atcgcatctg cctcgctcaa cgcttttaat agcgataaat ccattatgcc tctcccgtga
    27241 gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggta accagcacaa
    27301 catcttcgat gcgcacgccc ccttgcccta gcaaataaat ccccggctcc acggtcagca
    27361 acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggatct tcatggactt
    27421 caatgccgat agcgtgacca gtgttatgac cgaaatagtc gccaaatcca gcctcggtaa
    27481 tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac gccggggcga attgcagaga
    27541 ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc aaacagcggg tgagattcgg
    27601 cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagcagta gccctgatac
    27661 agcgcaccaa aatcgagagt gacaaactcg cccgctgcaa caatcttgtc gctggctttg
    27721 ccgtgcggca gcgccccgcg ccagccgctg gcgacaatgg tgtcaaaaga ggctttttct
    27781 gcgccctgct ggcgcataaa ccactccagt tcagcggcta tctcgcgctc gctcatcccc
    27841 gcctgaataa agcggcgaat atgctccgct ccgcgatcgg caatcccaca ggcgaggcgg
    27901 attatctcca cctcctctgg cgttttgatt tgccgcagca catccggcgt ggcgctaacc
    27961 agtttcgcat tgagttcaga cttccagcgg tgcgcggttt cccaactcac ctgctggcct
    28021 tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt gattgacgat agtggtaagc
    28081 gtgttcgttg cgtcaagcaa atgcagctgg tagccttgcg cacgggcttc cacctcaacg
    28141 aaatagcgcg aatctaccag aatgtgcgca ctttcacggc taataaccac atagcctgat
    28201 ccggtggaga tccccagatg cggctgtttg ttctgccgtg aggagagaag caccgcatcc
    28261 agttgttgca ccttaagcca gttgcgcagc gaagcgagta atgtcatctg gattttcctt
    28321 cttacaggct atcgattaac aatttgcctt tgcggaacat catcagacgc aggaacacca
    28381 ccatcagcgc agtaatgatt gcccccagcg cgatccccgc catataaacg cccaagttag
    28441 tgaccagcgg ccatgcccag atagcggatt ccgggaacca ttgcactgcg cccagccaga
    28501 cagctgcggt tgagccgaca atcgcgccga ccatatatga cggaatagcg gtgatggggc
    28561 tttccagcgc aaacggaatc gcaccctcac taatccccat aaaggccagg aacatcgcgg
    28621 tcttaccctg cggataaagt tgagcgttga acaggcgttt gccggttaaa cgacggtcaa
    28681 taatggtcgc cagacccaga ccaatcggcg gaataacgat agcgatagaa cgagcggtga
    28741 ccggtaatac gtggtcagtg gtaaagctga aggcaacgaa acctgcggct ttgttgatcg
    28801 ggccaccaag gtcgattgct gtcgccgcgg cgatccccat tgcgtacatc agcgcgccct
    28861 tctcacctgc ggcagtcagc acagtacgga taccgccgtt gatccagcca ccaaagggag
    28921 tgatgacgta gtacatcgcc agcatgacaa aaatggcgga aagaatcggt agcaaaaatg
    28981 tggttttgaa cgctaacagg aaatccggta actggatttt ctggttcatc catttcacaa
    29041 ggtaaccggc aacgatagaa ataatcagcg caccaatgaa ggtggacggc accggcgaag
    29101 aggtcgccca ttgcatagtg ctgggatcga agttcagcag ttgcgtcggc tgggtagaca
    29161 tcaacccgcc gataaaacca gccgggaacg ccagtttgcc accgatagag ttcgccacaa
    29221 atgcagcaaa catcggaatg gcaaaaccaa acaacacgcc gccgaaggac tgcgacagcc
    29281 aggcaaattt aagtaaggag aggtcgaaac cggagaattt cccgctatta agagcatcca
    29341 tgatgccgat ctcagcagga attttaagcc agctataagc aatcagctgg ctgaaagcga
    29401 ggatcacacc gcccataatt aaggtcggca ccatgcgtga aatccctgac atcacatgct
    29461 gcgggagttc accccagaag ctgcttttag aggcctgcgg attcttaact gccgctgccg
    29521 caccggatgc gccaggcaca acggttgcac tgcgtttttt aatggccata acaattatcc
    29581 ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc gatttctttg atgatgcccg
    29641 cagcgttttt aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat tcgaaacgtt
    29701 cgttatcttc cggggtaact gccacggaat ggatgatgat ggtcgcttcg gcgatatcct
    29761 gcgccgtcag gcgattctgg atgccgtccg cgccctgggt ttcaattttc acttcataac
    29821 cggcttctac cgccgcttct tccagcacct gagcggccat aaaagtgtga gccaggccca
    29881 ttgggcaggc acataaggca atcagtttct tactcatcgt taattcctcg ttcagtgata
    29941 gttgactagt gaaattatga acgtgcaaaa caaatcgccg ttaccagaca aataatgcat
    30001 ttactggaga attaatcccg ctgtacggaa gtgtgacgca ggtcggcctt gtgaggggac
    30061 acaaagaaaa attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga
    30121 tataacagga aggatttaca gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc
    30181 gagaagaccc ggatagtcat ggacgatgag atacaccgga atatcatgga catattcttt
    30241 aaagcgccct ttatcttcaa atgcggcacg gaaaccggag gctttgaaga actcaaggaa
    30301 gcgcggcacg ataccgcccg caataaacac gccgccaaat gtcccgagat tgagcgccag
    30361 attgccgcca aaacggccca taatgacgca aaacagcgac aatgcgcggc ggcaatcggt
    30421 gcagctgtca gccagcgcgc gttcggtaat atcttttggc ttgagatttt ctggcaggcg
    30481 gttgtcagct ttcacaattg cgcgatacaa attcaccagc ccagggccag aaagcacgcg
    30541 ctccgccgaa acatgaccaa tttccgcacg caatatttcg aggataatgg cctcttcttc
    30601 actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc
    30661 gacatggacc agatgcgcaa ccccaagccc cgttccggca ccgtaaaccg caataggctt
    30721 accttcgacc ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat
    30781 cgccatcgat acagcggtaa aatcgttaat aatttccaga tggctaaaac cgagattctt
    30841 tttcatttcg gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt
    30901 aattgggcaa gcgatggcaa tacagccgtc tttcacctcg accttatgtt cttcaagata
    30961 aacgcgaatg accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga
    31021 gatttcacca ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc
    31081 gactaatgca tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc
    31141 acactgtaaa tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat
    31201 cgatctgggt cacacaatta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc
    31261 aagctgggca aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag
    31321 ccagaaccat gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta
    31381 ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc
    31441 cgggaactct ggggatagcc caggattcac ccctctggat catcggtgta ttaacgctaa
    31501 cgggtattgc ggtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg
    31561 cctgtgaacc gctgatcggc gcaccggttc cgccctctgc gctacctaga cttatcgtag
    31621 cattaattct cggtcttgct ggcggcgtca gcctggggcc ggaacatccg atcatgaccg
    31681 tcaatatcgc ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg
    31741 agtggactat tttagcctct gccggaacca tcggcgcgct gtttggtaca cctgttgcgg
    31801 cggcgttgat attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc
    31861 tttttgcgcc gttaatggcg gcagcagctg gtgcacttac taccggatta tttttccatc
    31921 cccatttttc actgcccatt gcgcattacg gacagatgga aatgaccgat attctcagcg
    31981 gtgcaattgt cgcggcgatt gccatcgcag cagggatggt cgccgtatgg tgcttaccac
    32041 ggttgcacgc gatgatgaat caaatgaaaa atccggtgct cgtgctgggt attggcggat
    32101 ttattctcgg aattctgggg gttattggtg gaccagtttc gctgtttaaa gggctggatg
    32161 agatgcagca gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg
    32221 taattaaact tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct
    32281 tcccggcagt gtttgtcggc gtggcattag ggttgatgct gcatgagcac gttcccgccg
    32341 ttccagcggc aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg
    32401 atggctggtt aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc
    32461 tgctctgtat cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg
    32521 tcaatcgtcc gaagcaacag ccaccccacg ataatgttta gcaaaaaatg ctctccttta
    32581 tgatttaaga gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag
    32641 gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg
    32701 ccgccctgat ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc ctactgccat
    32761 cgatcaaatt acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact
    32821 ggcgagatcg tgattactgg catcgcaatt atgaatggcg caaaaaccgc tggtggcgtc
    32881 atgataatgg ctatcatcgt ggctgggata agcgtaaagc atatgagcgt ggctatcgtg
    32941 aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg caccgccatt
    33001 aaacgcgtcc tccaatggag cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct
    33061 acaaaccggc tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt
    33121 gccagatgcg acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat
    33181 tcagcgccac gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca
    33241 ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg
    33301 cgataccaaa acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca
    33361 tcagaatgac aataaatgac ggcaacatgg tgactgtacg acgcacccac agcgggatat
    33421 ggaagcgaat gaatccctgc atcaccacct gccccgccag tgtccccacc accgttgagg
    33481 acagtccggc agcaaccaga cttaacccaa agaccgttgc cgcagcatgg cttaacagcg
    33541 gttgcagcgt cagataagcc tcatcaagat cggcaacacc agtatgacca gaaaagtgga
    33601 acgccgccgc agctgtagcc atcatcacca gattgacaaa accggcaatc gtcatggcga
    33661 tagccacatc ccatttggtg gcggaataac gttgttgacg cgaaccgcca tgtaaatgct
    33721 gagtgagcga agagtgcaaa taaatcacat gcggcataat cgtcgcccct aacacgcctg
    33781 ctgccaggaa gaccgcctcc gaagtaggta aactcgggat caccattcct ttacccagct
    33841 gcgccaggtt aggctgggag aaaatcaact cgacaatgta agccgcggca acaaacaaca
    33901 gtaacccgcc aatcactttc tccagcggtt tttgcccgcg acgttgcagc attaaaatca
    33961 ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa cgaaacacca agaatgagtt
    34021 taaaaccgat cgccgcacca ataaattccg ccaggtcggt tgccatcgca ataatttctg
    34081 cctgaaccca atagaaccac actacgggac gcggatagtg atcacgaatc tgctccgcca
    34141 gatttttacc ggtggcaatc cctagtttgg cagagaggat ctgaatcagc atcgccatca
    34201 ggttggccca aacgacaacc cacagtagct gatagccgaa gctagcaccc gcctgaatat
    34261 tggtcgcaaa gttaccggga tcgatataac caatcgccgc aatgaacgca ggtcccatta
    34321 atgcgagcct catcttacgc gccgcccgtc cgctgctact ctcaacgcga tagttcgtca
    34381 tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc tatgctaaac gagaatgatt
    34441 atcaaattca tttaaatgga ttgtggtgat ttctctgata gaccaagatt atgattacga
    34501 aaagattgat gacttgaatg ttatggttat gtttaatgtt agcacattta cataactttc
    34561 agcttccata cacaacatag cagaaatgta tgacagatca ctatttttga agcctgtcac
    34621 aggacgtcat tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg
    34681 cacggaaatt taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta
    34741 ctggcacttg ccgttgttgt gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt
    34801 cgtatccgtt atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg
    34861 aactccgacg ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc
    34921 ggatttgcca acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca
    34981 ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct ctgcactgat cggtattctc
    35041 cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc
    35101 aacggtatgg gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtca
    35161 gaaaacttta ttgcctataa agatatcctc ggcaaaatct cccgtaatcg tatgtacacc
    35221 atggctgcca cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg
    35281 ctggaaccga aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg
    35341 ctgtcgctga tcaatcctta ccgtgttgat gccagtgaag aaaacatcca gatgtccaac
    35401 ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa
    35461 gttgccatta tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac
    35521 gcactgtttg ccaccgttac tggctggttt ggctacagca tctccttcca gggcatcctg
    35581 ggctacatct tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa
    35641 gtgggcagta tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg
    35701 cagaaaattg cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctagtt
    35761 tccttcgcta acttctcttc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa
    35821 gagcaaggta acgtggtttc tcgcttcggt ctgaaactgg tttacggctc taccctggtg
    35881 agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaaa accatacata aaaaagccgg
    35941 ggatcattcc ccggcttttt tacgcctgtt aatcagctaa tggctgcggg cgaccaatca
    36001 aatacccttg cagatattgc accccgagct tatgcaatag cgcctgctgc tgttgcgtct
    36061 cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc cagatcggta attgatctca
    36121 caatcatcgc atccagcgta ttcgtgacaa tatctttcac aaagacgcca tcaattttga
    36181 tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc atatccggtg ccaaagtcat
    36241 caatcgcaat ccggaaacca aacttatgca gctgctcgat gttgtacatg ctgctttctg
    36301 cgttagaaaa cgcctgctcc tcggtgatct caagaatgac cgcctgcggg gagatgtgat
    36361 aacgcttaaa cagacgaata atccgcccgg caatattctt ttgcagcagc gtgagcggca
    36421 ttaaattgac tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc agccacttca
    36481 acaaggattc cagcacttgc aaatcaaaac gcgcgctaag gttaaactga gcaataaggg
    36541 gcagaaactt atccggggtc ataatgccgc cgtcatattt cagtcgcgcg aggatctcat
    36601 cataaccttc accctctttg ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat
    36661 ctaacgccgt gcgaatggta ttcagcaata gcacctgttt agtggtctgc ccggaaacca
    36721 tctcctctct gctatccagc gccagcacat gatgatgtgc gcaggattgc tccgccagcc
    36781 agcttaactg ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg
    36841 cagcaccata gcccatatcc agcccggtat tgttccagtg aatttgccga ctattcagga
    36901 tgttaaccat atgctggagt cgcccttccg tttccggccc gctcagcact aacagcagtt
    36961 cactacccgg caattgatac aacttttcgt tttcctgcat caacggcagc agcgtacggc
    37021 aaattgagcg gatacagtga acgcgcatca ttaagccgta atgacgactc ataaactcaa
    37081 gattatcaat gcgcaggcag caaaaactct tgcccgcctc ttgctccggc gcttgttcca
    37141 acgcacgaaa gttgggtagg agcgttaacg gatctgttag cgcctgcaaa tgccagcggc
    37201 gattaagcca ttcactgcga tgataaatgc gcaccatata gagcaggcaa acgctaaagg
    37261 aaatcagcac cgcgagaata aatgccagcg aatattcggt ttccacccct tgcaaaaagt
    37321 tctgattgta attcagaagg caaagcgttg aaacagccca ggtgagattt aaaaacggat
    37381 agcgaagatt accgaccccg agggtaaaga tgatgaagaa aacgggtacc aggtaaccgg
    37441 caataaagtc gttttcataa ggtgtgcaca acagaagtag cagcacgctt aacgctgcca
    37501 gccagcttaa ggtaaatgcg agtgagatag tagaaaagca tgttgtaaat cagcactgcg
    37561 gtgaaaaggc ttagtaaatc aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggtg
    37621 gatatcttta gcgggaaatc aaagaagctc cccacaagat acatgctgca tttgatgccg
    37681 atcggcgtca ccaaaccaag ccagaacaga cgttgccaga catatcggct ggtcaatcca
    37741 taacgccaac gcgtccccag ctgccagcgt aatattgcgc aggcacacaa cacgacaaac
    37801 gtctggctgc cgagcatgac cgcagtctgt aaaaaagaga gatgaaagtt ccacagattg
    37861 gtgcaaaaca tcccgcataa aacgggaacg acaccgcgcc agccaaagat aaacaagatt
    37921 gacagcatta cgcacagtgg catccacgcc agaaagatat aactggaatg gataatggca
    37981 agtggcgaaa taaaacggga tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg
    38041 aatatcttga tattttttat caggttatgc tccacaaaca tgagtacact actctcactg
    38101 atcggggttt tcaggcacac aaataataag tttattcgca tcgcgaagca aacaggggcc
    38161 agcctgttgc cgatttttca tccaatgatg ctttcaccag gcggcgctaa cccggacgta
    38221 gactgtatta caaaagcggc aaaaagcaga gacaaaaaac ccccgctttg cagcgagggt
    38281 tggaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg
    38341 ctctcccagc tgagctatag ccccacgatg cgtttacgta ccaaatttgc tgggtgcaaa
    38401 atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc
    38461 ccagctgagc tatagccccg tcacgtaaag cttgtcgagt tgacgggcgg catcatatga
    38521 attccgcccg aatgtgtcaa cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca
    38581 ggcaaatgaa caattttgga aaaccgctcg cattcagtag ttattcatgt cacggtttcc
    38641 tgtaaagtgg tgttataaaa tgaactacta atagacccac atacattcag ggaattgtta
    38701 tgttcaagga gaggatgaca ccagatgaac ttgccagact gaccggctat agccgccaga
    38761 ccattaataa atgggtacgc aaggaaggct ggacaacgtc accaaaacct ggcgtccagg
    38821 gtggcaaagc ccgactggtt cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg
    38881 aacgtccaga aggtcaggga gaagcgcctg ccctttctgg tgatgcgccg cttgaagtat
    38941 tgctggtgac actggcgaaa gagatgacgc cagttgaaca aaaacagttt acatccttgc
    39001 ttctgcggga agggattatc ggattgttac aacgcttagg gattcgcgat agcaaataat
    39061 atgaaaagat tacgcaataa aatgaccacc gaagaactgg ctgaatgcct cggtgtggct
    39121 aaacaaactg ttaaccgttg gaccagagaa aaaggctgga aaacggaaaa atttcccggc
    39181 gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc aagtttgcga gtttattcag
    39241 aacacgccag ccttccataa cacaccaatg ttgatggaag ctgaagagag aattgctgaa
    39301 tacgctcctg gagcacgcgc cccggcgtat cgacaaatca tcaacgcgat agataatatg
    39361 acagacattg agcaggaaaa agtcgcgcaa tttttgtcac gtgaaggaat tcgcaatttc
    39421 ctcgcccgtc tcgatattga cgaatcagca taaataaaaa acggcaggat attatctcct
    39481 gccgtttatc tttttacacg ctaattactg ctgattttcg cgttcagcaa taaaatccag
    39541 cgctttgttg atacgctcga tactgcgggt cttaccaatt gcgtgaacgg taacatccag
    39601 tgctggagac tgccccgcac cggttacggc gacacgcagc ggcataccaa ctttacccat
    39661 acccacttcc agctcatccg ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca
    39721 gtcagtaatc gcggccagtt tgtcacgaac cacttccagc ggctgacgcg ctaccggacg
    39781 cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca
    39841 gctctgtgcc atctctttca gcgtcttgca gcgttcgccc agcagtttca ccagatcagc
    39901 cagctgcggg ccgttacggg tatcgatatt ttcctgctca atgtgccact gtaagtgagt
    39961 agcaacatac tccggcggca gcgcgttaat gtagtgatgg ttcagccaca gcagcttgtc
    40021 ggtgttgaac gcactggcag atttgctgac ggcattcaaa gtgaagtatt tgatcatctc
    40081 ttcacgagtg aagatttcct gatcgccgtg ggaccagccc agacgcacca gatagttcag
    40141 cagtgcttct ggcaaataac cgtcatcacg atactgcatt acgctgactg ccccgtgacg
    40201 tttggacagt tttttaccgt catcgccatt gatcatagaa acgtgcgcgt aaaccggcac
    40261 cggcgctttc agggccttaa gaatgttgat ctggcgcggc gtgttgttga tatggtcttc
    40321 gccacggata acgtgggtga tttccatatc ccagtcatcg acaaccacac agaagttata
    40381 ggttggggaa ccatcggtac ggcggataat aagatcgtcc agttcctggt tgctgaactc
    40441 gatcggacca cggatctgat cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg
    40501 tacaacacac ggttcatcat cagcatgatg ctcatggctg tggcggcagc gaccgtcata
    40561 acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc gcttccaggc gctctttaga
    40621 gcaatagcat ttataagcag tgccctcttc cagcatctga tcgatcaccg cgttgtagcg
    40681 atcaaaacgt ttggtctggt agtacggacc ttcatcccac tccaggctca gccagttcat
    40741 gccatccata atggcttcga tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat
    40801 acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg
    40861 cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg aagcgagttt tgattttcat
    40921 gaaatggcct tacgtttaga aagatgccga caaccggcaa atcctggaaa aattgagtgg
    40981 gcgatattct atcactcccg cctgattcct caatgtagtt cgggtttcta ccttacgcat
    41041 tattgttttc gttgacaaat tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt
    41101 tacgacgaac gaacaatttc tttagaaaat gcgttgactc attttgaact ctccctataa
    41161 tgcgactcca cacagcgggg gtgattagct cagctgggag agcacctccc ttacaaggag
    41221 ggggtcggcg gttcgatccc gtcatcaccc accaactact ttatgtagtc tccgccgtgt
    41281 agcaagaaat tgagaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag
    41341 ggggtcggcg gttcgatccc gtcatcaccc accacttcgg gtcgttagct cagttggtag
    41401 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa
    41461 aaaagcgccc taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc
    41521 aggtttgagt tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga
    41581 gcgaagcgag tcatcctgca cgacccacca atgcaaaaaa gcgccctaaa ggcgcttttt
    41641 tgctatctgc gatttgtaag attgattttt cttctcaagt tccttgttct tctaccgata
    41701 ttacctcgta cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt
    41761 ccatacaaac acagagtagt aacagggcat cacaaggtag cgatgttgct tcacaaatcg
    41821 cgcggataag tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta
    41881 gcggcagtgc tgaagacaag caaaaacagg ccgaattgat tcaacagcaa atcaccctac
    41941 tggaaacaca actggcgcag ttgcagaaac agcaggcaga aaaggcgcag gagaaagagc
    42001 agcgtttgtc tctgaatgta tcccttctaa atcctgttga aaaaacgact catatcgata
    42061 tctatatctg atacaacgga tccccttccc gaccagccat tagcgcattg ccctggtctc
    42121 gtaccacgtc ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca
    42181 gcatcaactg gcgttcaacc accggcgtaa tgcgcttaac caccagtggg ctaccttcag
    42241 gcaatggtag cgccagtgca ggaagaatac tgatgccgat gcctgcggct accatcggga
    42301 acagcgtcgc cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca
    42361 gcgctgcgtc aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc
    42421 cctgtaatgc ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa
    42481 gcagaaagaa aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat
    42541 cgatgacgat gccaaagtcg acatctccct ggcgaatact ttccatcacc cactgctgcg
    42601 gacgatcatg caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt
    42661 gcggaataag atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt
    42721 gttgccccat acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa
    42781 gacgcaaagc caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca
    42841 gcaggcgaac accagtatga ttttccagct ccttcacact gtgacttact gccgactggc
    42901 tcaagccgat acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa
    42961 cctttaattg ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca
    43021 attttatttc tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg
    43081 aaactttttc gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc
    43141 tctttctttc cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct
    43201 attgccctgc tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc
    43261 ggtggtcact ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt
    43321 ctgggtgtac tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt
    43381 tttctctact tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg
    43441 gcgggcggta acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt
    43501 ttcctttcac cattgctggt tggtctggtg atgaatgttc acggtgcaag gggcagcctt
    43561 gagcaggtcg gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc
    43621 cggccgtgga ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag
    43681 acgtccattc tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg
    43741 cataaagttg gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct
    43801 atcgtgattg tagttaacgt ctttatggca cgccgactga gcttcaataa ggcagatgaa
    43861 attactatcg tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac
    43921 attctgttcc ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag
    43981 atccaattga tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta
    44041 caggcgcagc aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gtgctggacc
    44101 agttgcgtca gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc
    44161 cctgccggga actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcaatt
    44221 aagcgacgcc ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc
    44281 tctttttcca tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat
    44341 gcccagttcc tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc
    44401 ggttttcttc gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc
    44461 gtcatcacgc gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg
    44521 gctgtcaatc tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa
    44581 cagctcgctg atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc
    44641 aacgacaatg ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcgc
    44701 ttccagcgtg ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat
    44761 gccaagtgca tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac
    44821 cacgttttgt gccaattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag
    44881 tttgaacaga tctgccggag tatggacata ttctttttca accagctgat cgatgatttt
    44941 gtcgcctatt ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt
    45001 acgctgcgca ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg
    45061 ctcaacgtca gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc
    45121 cgggcgttca gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat
    45181 caccacttta tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt
    45241 tgcgttactc accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat
    45301 cgcgccagta cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc
    45361 cgggaattta aacgcaaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg
    45421 ctgttccagt gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg
    45481 gtcttcttcc actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac
    45541 ccgatcgctg accggcaccc cccacttttt aaattgcagt aaacggccaa gatgagtatc
    45601 cggcagctcg ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt
    45661 cgctgtaata cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc
    45721 aaaaactttc ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg
    45781 caggaacact tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag
    45841 cggaatggcg cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc
    45901 acgggtcgcg gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc
    45961 cagcttcagc tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg
    46021 tttgttgaaa gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac
    46081 ttcatggcgt atctggctga aagctgccag cggcgcagca cctacacgtt gggtaggcga
    46141 atcaggcgta atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct
    46201 gtcgtattca gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg
    46261 gcaaagcgtc gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca
    46321 atgctaaaaa cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc
    46381 aggcgttggc gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag
    46441 tcatcatacg gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg
    46501 cagattgcag catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa
    46561 agatagtgac acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca
    46621 ccatattcgc caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt
    46681 aaatattcat atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac
    46741 cgtttagctc gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct
    46801 tcggtttatc cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg
    46861 gttctgcagg ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg
    46921 ctggcgcgac ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg
    46981 caggctgctg caccggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag
    47041 gcggctgctg gaccggttga cgcggctgcg cagacgcata aggcggtagg tactggtgtt
    47101 gcggcgacgg acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt
    47161 gaacacgaac ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt
    47221 cacgttttga cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac
    47281 ggctggtcca gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta
    47341 atatcagacg caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg
    47401 gcaaacattt actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac
    47461 tttcacagca caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa
    47521 ctgtccattg cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg
    47581 agctcatcct gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt
    47641 ttgcgcaagg ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc
    47701 tgctggtcaa tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg
    47761 tctggatccc gactttcatg agttacgttc cggactggct acaatggctg agttatctgt
    47821 tgtggcctct ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg
    47881 ctaactggat tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga
    47941 ctggcgctac accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca
    48001 tgaaacgcga atggcaaaaa tttgtctggt atctgccgcg cgcaattgta ttactaattc
    48061 tttacttcat ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg
    48121 cctggatgtt agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta
    48181 aagagatgcg caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa
    48241 ccagcctgtt tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg
    48301 gcgcgacggc gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat
    48361 ctaccggtta ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt
    48421 ccatcttgca tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca
    48481 tattctctga gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc
    48541 atgagtaaga tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg
    48601 aatcgcatcg gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc
    48661 gttaagtgcc gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa
    48721 ccaggcgttg aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta
    48781 gctgccgctc gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc
    48841 cgcaagctgc tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg
    48901 aaaggcgcaa tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg
    48961 ctgcaacaat tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag
    49021 atctgggaag ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt
    49081 acgctgactg gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct
    49141 gtcgccgttg agccaaccga ttctccagtt atcgcccagg tgctggcagg tgaagagatt
    49201 aaacctggcc cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat
    49261 ctcaagctgg tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt
    49321 cgtctaatgg aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgca
    49381 gcgttgaaac tacaagaaga tgaaagcttt accaacaaga atattgtggt tattctacca
    49441 tcatcgggtg agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa
    49501 ttgcaacagt aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt
    49561 ttgtggcctg cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta
    49621 tttaaccaga ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg
    49681 aatcgatttt atgatttggt tcaattcttc ctttagcggc ataatgttta atgacgtacg
    49741 aaacgtcagc ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt
    49801 ctgttaaaaa ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt
    49861 tccacaacac taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta
    49921 ccgctccgaa cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct
    49981 tcacttctga aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta
    50041 aactgcagac tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag
    50101 acgagcagaa agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg
    50161 ggttctttta aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc
    50221 cccgggtatc gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg
    50281 gaaaaaaatt tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc
    50341 caaggcatca gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga
    50401 aaaagaagcc atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga
    50461 aatcatagcc ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat
    50521 cgaaggtcag gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc
    50581 tgacgtacgt gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga
    50641 cctgagcgcc attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac
    50701 cgcacagctg aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc
    50761 ccacacctct atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt
    50821 cacctctcag gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta
    50881 cgtcaatcca accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc
    50941 tgaaaaagca gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca
    51001 ggtagaagta tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg
    51061 cgctgaaggc gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc
    51121 cactgaagaa gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc
    51181 ggttatcgtt cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc
    51241 gaaagaagag aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga
    51301 gatcctgcgc gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat
    51361 tatgttcccg atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat
    51421 ctacaaacag gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt
    51481 aatggtggaa acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt
    51541 ctttagtatc ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga
    51601 tatgatttca cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt
    51661 tattgatgct tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga
    51721 tgaacgtgct acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat
    51781 ttctatcccg cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt
    51841 agcagagcag gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt
    51901 cattgaagaa aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga
    51961 agatcatggg tttgttcgat aaactgaaat ctctggtttc cgacgacaaa aaggataccg
    52021 gaactattga gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg
    52081 atgtcgtttt tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca
    52141 aaatggtcgc gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct
    52201 ctatcgaatc tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac
    52261 tgaaaggcga aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata
    52321 ctgtcattga atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg
    52381 ttgttatctc caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg
    52441 tgggtgaaac cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc
    52501 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
    52561 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
    52621 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
    52681 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
    52741 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
    52801 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
    52861 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc gccagcggca
    52921 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
    52981 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
    53041 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
    53101 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
    53161 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
    53221 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
    53281 cgcttccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
    53341 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
    53401 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctattac aaaacaaaga
    53461 taaggtaatt cagtgaagaa aatcatttgt ctggccatta cgctgctaat gacactcccc
    53521 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
    53581 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
    53641 gtttcccacc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
    53701 tttattgata aggtagcttc gtcgtcatcg attaccggga agccgtatat cgtgaagatc
    53761 cccggtaaga gcgatgagaa cgcacagcct tttttacatg cgttaattgc gcagacggat
    53821 aaaacggtgc ctgcggaagg aaattaatcc gctttggtaa ggcatttaca ggaggtaaca
    53881 tgaaaaaatg ctttatttat cacgatgaaa aatcgaataa attttggtgg atagattacg
    53941 aaggggatag tttagctgtc aactatggca aggtaggtag tattggtaaa ttccatacaa
    54001 aagaattcga taatgaagaa cagtgtctga aagaagccag taaattgatt gccgcaaaaa
    54061 tgaaaaaagg atatcaagaa actccagact ttaacttcat ggatctctac tattttgatg
    54121 atgaagagat tgggttacat cttaaaacgt cacacccaaa cttccagtgc cattttactg
    54181 atccacttta tatgtgttgc tgggatgaag aatctccttt tggtagcgat gaaggtgctg
    54241 atgctctaaa cgaacttgaa aatagcctcc gttctggact gtgctgattt ccctcaaatg
    54301 ttaattgaaa ctatgtgggg tatgaaatac atcgctatgg acagtattct tgaagaggat
    54361 gtgcgtgcgc aattactagc cgatgaaatg agctctatcc agagcaatat gattacctac
    54421 gcgactgcat tcggtcagat caaagtcatg ggtaaaatct cccataaact taaaaagatg
    54481 ggactcaatg cactagcgcg tcatcagctt accgcaaaaa ttcttcaatg gggtgacggt
    54541 caggactcac caatacttca aaaaatgatt gatgacctta ctgcttttcc acacgaaaat
    54601 taaaaactgc atttgtcggc agcaacaact gccgacaaat cagattattg acaaagtgca
    54661 ctttgtttat gccggatgcg gcgtaaacgc ctgatccggc ataaaaatat tgctaattca
    54721 atatattgca gaaacattgt aggcctgata agcggagcgc atcaggcagt tttgcgtttg
    54781 tcatcagtct ccaatgctat taatccttaa atccctgccc cctgactaaa atgctcttcc
    54841 ccaaacactc cggtggagag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc
    54901 gcaccagggt tagcttttgc cacccgcagt gctccagcaa ccgcgccgcc tgagctgacg
    54961 ccacagaata ttccttcccg caccgccagt tcgcgcatgg tgttttccgc atcgcgctga
    55021 tgaatatcca gcacctcatc caccagcgca gcgttgaaaa tccccggcag atattccgca
    55081 ggccagcggc gaatgcccgg aatactgctg ccttcttccg gttgcaggcc aacaatggtc
    55141 accggtttgg attgttcgcg cataaagcat gaaacgccgg tgatagtgcc ggtcgtcccc
    55201 atgctggaga caaaatgagt gatgcgcccg ccggtttgct gccagatttc cggcccggtg
    55261 gtggtgtaat gcgcataagg gttatcggga ttattgaact gatcgagcag ctttccttcg
    55321 ccacgattcg ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg
    55381 acaagaatca gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg
    55441 tcgggcatca gcaatttcat gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg
    55501 ccggtgttac cactggtggc ttcgattaag acatcacccg gtttaatttc cccgcgcttt
    55561 tccgcctcga cgatcatcga aagtgccgca cgatctttca ccgaacctgc cgggttattg
    55621 ccttccagtt ttaaccacac ttcactgccg ttatccggcc ccattcgctg caacttcacc
    55681 agaggcgtat tgcctattgt ttgttctaat gtactcacaa tctctatcca tacgtggtgt
    55741 tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg ttacaaacct tgccattaaa
    55801 aagcccggat cgcgggaagc gcctccgggc gtttaacatt cactcaacct atcaggcgct
    55861 ttgtgcgaga gcaagttcct catcgcgggt ttcaatacgc tcgtcgccgt tatacagccg
    55921 cgcatgttgc agaccaacga ataaacgctc gccatgctgc ggggcatcgt cgccatgcat
    55981 cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc accactaatt gggtgtagtg
    56041 acctttcggg ctggcttcca gtacctgtac cggcagcggc gaatcgagac tggtacggcg
    56101 gctgatatcc acttcccacg ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt
    56161 gtagcccagc ggccagcgat gcgcgccaac atggaactgc ccgccgcgaa tggttccctg
    56221 caggcggttc acttcgccca taaattcgag tacaaaacgg gtcgctggtt cgcgccatac
    56281 ctgattcggc gcatcagcct gttcaatatt cccctgactc atcaccacta cacgatcggc
    56341 tacttcggtc gcctcttcct ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc
    56401 atgcagttga cgcagccagc gacgcagctc tttacgcacc tgcgcatcca gcgcgccgaa
    56461 cggttcatca agcagcagaa tttgcggttc tacagccagc gcgcgcgcca gcgccacacg
    56521 ctgtttctgg ccgccggaaa gctgcgccgg ataacgatcc gccagatgcg caagctggac
    56581 catttccagc aattttgtca ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg
    56641 cggcagcacc gtcaggccaa aagtgatatt gtcgaatacc gtcatatggc ggaacagcgc
    56701 gtaatgctgg aacacgaaac cgactttacg atcgcgcgcg tgcaggcgac tcacgtcggt
    56761 gccgtggaag cgaatatgcc cgctggtttg atgctccagc ccggcgataa tccgcagcag
    56821 cgtggttttc ccggaaccgg acggccccag caacgcgacc atctgaccag aaggaatatc
    56881 cagtgagata tcgttcagca cctgggtgcg accaaaagac ttcttgatat tggcaatctc
    56941 aatgctcatg atgctcctcc tgctgcgcgc gtttttcctg attctccagg cgccactgca
    57001 acatactctt taaaaacagg gtgataatcg ccatcaacgt caacagcgcg gcggcggtaa
    57061 aggagccgac ggtgttgtag tcctgctcca acaattcaat ctgtaacggc agcgaaagtg
    57121 tttcgccgcg aatcgagccg gaaaccaccg acactgcacc aaactcgcca atcgcgcggg
    57181 cgttggtcag caccacgccg taaagcagcg cccagcgaat attcggcaat gtgacgcgac
    57241 ggaacatctg ccagccggac gcgccaagca aaatcgccgc ttcgtcttcc tggctgccct
    57301 ggcttaacat caccggcacc agctcgcgca ccacaaacgg acacgtcaca aagatggtga
    57361 ccagcaccat tccgggccag gagaacataa tttgcagatt atgttcatcc agccagccgc
    57421 cgagcgggcc gttggagccg tagaacagca gatacaccag accggcaacc accggcgata
    57481 cagcaaacgg aatgtccagt agcgtcagca gtaactggcg tcccgggaag ttaaagcgcg
    57541 tcaccagcca ggccagcaga atgccgaaca ccaggtttac cggtacggca atcagcgcga
    57601 tcatcaccgt cagccagatg gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg
    57661 gcatcagccc cttgctgaat gcctgcacga agatgtaaat catcggcacc agcaggatga
    57721 acgccgaaac cagcatcccg atgccaatca gaaaccattt gccccagtta atcgggcgcg
    57781 cgtcataacg cttcaattgg gtaacttccg ccattaatga cctaccacac gccgaccaaa
    57841 gcgactttgc agagtgttaa ttgagaacag cagcagcaga gatgccgcga ggatcaccga
    57901 agcaatcgcg ctcgctgccg ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga
    57961 cgtcacttcc gtcttccacg cgatatttcc ggcgataaaa atcaccgcgc caaattcacc
    58021 aagactacgg gtaaacgaca gcgccacgcc cgccaccagc gccggagaaa gctccggcag
    58081 caccactttg cagaaactct gccagcgcgt tgcaccaagc gtttccgccg cttcttcata
    58141 ttccgggcct aactcttcca gcaccggctg cacggtacgc accacaaacg gaatgctggt
    58201 aaaggccatc gccaccgcaa taccaagcca ggtataggtg actttgatat caaacttcgc
    58261 cagccattca ccgtaaaaac cgtttacgga aaagagcgag gccagcgtta aaccggcgac
    58321 tgccgttggc agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa
    58381 gcgataacgt gttaaaatcc acgccatcag cagaccgaaa acgccgttaa aaatcgatgc
    58441 cacaaacgcc gacagcagcg tcactttgta ggccgcgacc acctgcgggt tggtgatcac
    58501 ctcccagtac tgcgcccagc tcatctcaga aagctgcatc accagcgcag agagcggcag
    58561 cagcaatatc aggcacacaa acagcagact ggtgccaagg cttaaagtaa agcccggcag
    58621 cacgcgtctt gaagaaactg caaacattac ttacgccccg ccgctaacag cttgtctaac
    58681 tcgccgccgc tggtgaagtg ggttttcatc acttccggcc atgagccaaa tttgtcttcc
    58741 acgcggaaca gttcggtctg cgggaattta tctttcagtt tgtccatgac ttccgggtta
    58801 ttcacacggt agtaatagtc ggtgatgatg gtctgcgcct gtgggctgta gagccagttc
    58861 aggtaggctt ttgcggcttt ttccgtaccg ttggcctgca catttttatc gacccacgcc
    58921 accgggaatt ccgccagaat attggttttc gggatcacca cttcaaagcc ctgtgcttca
    58981 tactgtttac ggatgttgtt cacttccgat tcaaagctaa tcagcacatc gcccaagcca
    59041 cgctcggcaa aagtggtggt tgcgccacga ccgccggtat cgaacacttc aacgtttttc
    59101 aggaactggg tcataaactg ttcggttttg gctttatcgc caccgtcagc tttatccgct
    59161 gcgccccatg ccgccagata ggtataacgc gcgttacccg acgttttcgg gttcgggaaa
    59221 atcagcttca cgtcggagcg caccaggtcg ttccagtcgt ggatattctt cgggttgccc
    59281 ttacgcacca ggaagcccat ggtggagtag aacggcgagc tattattcgg caggcgcgac
    59341 tgccagtcag ccgggatcag cttgcctttg tcgtgcagga tttgtacgtc ggtcacctgg
    59401 ttataagtga caacgtctgc ttttaagccc tgcaaaatcg ccagcgcctg ttttgatgac
    59461 ccggcatgag attgttttat cgtcagtttg tcgccgccgt tatcttttgc ccattgctgt
    59521 tcaaacggcg gattcagggc ggcaaacagc tcgcgggaga cgtcataaga actgttcagc
    59581 agttccgttg cctgtacatg gcccgccagc agcagagaag cgaccagcgc gagtgagttc
    59641 tttttcagta agttaacggc cattgcgcac ccttataaat ttaatgactt tctaatagcc
    59701 atcatattta taacggacgt gaaaggagta acggttttat ataccgtttg gtgatttgga
    59761 agttaaaaag ggaataagaa cttggttcaa caggcgtaat tgttgcaggc agtttggaca
    59821 cggacagcgc ggagaaaccg gagcgtacac atagtacgtg aggatttcga gcactgcccg
    59881 gggccaaaat gacaaatgaa atagcctggt gaacttagtt caagacgaaa tcctccccac
    59941 acatgcaggg aggaaacaga ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg
    60001 ctgccgccat caatcacatt ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg
    60061 aaggccgcca gttcgccgac ttccagcgga tcggcgaggc gacacagcgg gattgctttt
    60121 gccatttcag tcagcaccga ttctggatct tccgggttcg actggcgggc aatgctttcc
    60181 gccattggcg tgcggacgta tcccgggcag atggcgttaa cgcgaatacc ggattgcgcg
    60241 tactccaccg ccagcgattt agtcaggcca acaatcgccg ctttcgttaa ggcatacgcc
    60301 gtttcgccag gatcggccac catatcacca gtgactgaag acatcatcac aatgcgacca
    60361 tctttgcgcg caatcatctc cggcagcacc gccttcgtga cgttccatac gcctttaata
    60421 ttgatatcaa tatggaaatc gcgatcttca tcgctcatat cgaggaaact gcccagacga
    60481 caaacgcctg cgttattcac caggatatca atgcgccctt ctttttcctt cgcgcgtttg
    60541 atagctgcgg ctaccgacgc cgggtcacgc acatcggcga caaccgccgt acagcgatga
    60601 ccacgaccac acagttcgtc cgctagcttt tcgatctcag gggagatatc cagcaagatt
    60661 aggttcgcgc catgacgtgc aaaagttctg gcaattcctt cgccaattcc ctgcaatgcg
    60721 cccgtaatca gtgctgtctt gcccgtgagt ttacccattt taatgctctc cttgttaata
    60781 cagagcgtta cactccattc cctctgaaaa tacaggataa acaaaatatt gctgcggatc
    60841 agctcactaa ttcaggaaaa tctcaatcca gtcgctgctt tatgcatcat ttcaggcgtt
    60901 gtgttagctc actgctgcgc tgaatcattt tcaatgacgc cacatcattg atgttgcaca
    60961 agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg tgcgggtaga catcgacgag
    61021 ctacgccatt cggtttcacc ggaaacggta tcgagcgtga aatgcgccag ccgccgcagc
    61081 ggagagtcag ccagagaagt aatggcaatc accgttgccc cctgctttcg tgccgcttct
    61141 gcgcacaaga caatctcttt tttactgccg ctgtaagaga tagcgatttg tacatcgcct
    61201 tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt cggcttcgca ggccacgcga
    61261 tagccaattt tcatcagttt gaacgacaga tcgcgcccca ccagcgccga tccgcccagg
    61321 cccgttatct ggataaacgg tgctttactg atgacctcaa tgattttttg cagccgcgcg
    61381 tagtcgaaca acgcacaggt ttgctccagc gccagctctt tttcgcgatt cagtttgcgg
    61441 gcaatcactt ccagcgaatc atcactggta atcgaactgt gcaggtgcag cgccgtggca
    61501 ttcgttttct cccggctggc actgtattcg ccaattaacg ccatacgcaa ttcagtaaat
    61561 ccttgcgcgc cgagcttctg ggcaaatttc acaatgctcg actggctgat accgagctgt
    61621 ttcgccatct gccgtgaaga gacggatttc agctcgctca ctctggcccg cagaaagtca
    61681 gcaatttttt gctcgttccc ggtaaattca ctttccgcat tgcgtatttt cgtcaggtac
    61741 aacatcttcc actctcctgg gctggcagca aaaattccgc ttaagttctt atgacgctct
    61801 tcacactctg cgagtgtaga ctcaatgatt cctttaagtc tgtcaattcg ggatatatga
    61861 ttccatacat ctgcaattat ttgatcgtaa atagtaaggt caccaccgat gcaactagaa
    61921 aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa ttgaccgcgt atcgacgctg
    61981 gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cgcctgccgt tgatcgcgtc
    62041 ctgccggata tcgccgcggc gatcgatgtt atccacgccc aggtcagcgg cggcggtcgt
    62101 ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc cagcgaatgt
    62161 ccgcccacct acggcgtgaa accgggtctg gtggttggtt tgattgctgg aggcgaatat
    62221 gccattcagc acgcggtgga aggagctgaa gatagccggg aaggtggtgt taacgatctg
    62281 aaaaatatta atttaacggc acaggatgtg gtggttggca ttgctgccag cggtcgcacg
    62341 ccgtatgtga ttgccggact ggaatacgca cgccagctcg gctgccgcac agtgggaatt
    62401 tcctgtaatc cggggagcgc cgtttcaacc accgctgagt ttgccattac accgattgta
    62461 ggtgccgaag ttgttaccgg ttcttcgcgg atgaaagcag gtacagcgca gaaactggtg
    62521 ctcaatatgc tttccaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg
    62581 gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgttaaaaac
    62641 gccaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcctgcga gcgcaactgt
    62701 aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg cagaagctaa aaaacgcctg
    62761 gatcaacacg gcggctttat tcgtcaggtt ttagacaagg aataacccat ggccaaagag
    62821 atcagcagtg aacttctgaa caccattctt acccgtgtcg gcggaccggg aaatatcgcc
    62881 agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc actggttgat
    62941 cccaatatca aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga ccaggtgcag
    63001 gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag cgatgagcga gctgctgggc
    63061 gatgcaccgg tacaggatgc cgcagaaatc gccgcccaga acaaacgtca gttaaaagcc
    63121 aaacaaacct ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg
    63181 atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat tgccacggtg
    63241 atgcacgttc cggcagatgc tcagggcacg ctacccgacg cgctgaattt tatgaaggtg
    63301 ttcagcaaag gtttgttcac cttcctagtg attctggtgg gctataacgc cgcccaggca
    63361 tttggcggca cgggcgtcaa tggcgcaatt attgccgcac tctttttgct cggttataac
    63421 cccgctgcaa ccaccggtta ctacgccggt tttcacgatt tctttggtct gcccatcgat
    63481 ccgcgcggca atattatcgg cgtgctgatt gccgcgtggg cctgcgcccg cattgaaggc
    63541 atggtgcgcc gctttatgcc ggacgatctc gacatgctgc tgacctcgtt aatcaccctg
    63601 ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggtggctg gttattcgaa
    63661 ggtatgtcat ggctgtttat gcacctgaac agtaatccgt tcggttgtgc ggttttagcc
    63721 gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc tgtttacctc
    63781 gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat ggcaggcgcg
    63841 ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc aaccgcacag tgcgctacgc
    63901 agtcaggtac gcggggcgat tattcccggc ctgctgggcg ttggtgaacc gctgatttac
    63961 ggtgtcaccc tgccccgcat gaagccgttt gttaccgcct gtttaggcgg cgcggcgggc
    64021 ggtttgttta tcggattgat agcctggtgg ggtctgccga tgggcttaaa cagcgccttt
    64081 gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat ccttccggca
    64141 atggcggttt atgctggcgg gattctggtg gcatgggttt gcgggtttat tttcaccacg
    64201 ctctttggct gccgtaacgt caatctggac tgatattatg aaacggacaa tgctctatct
    64261 ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa taccctgttc tgacagaagg
    64321 tagttgatca agtatatgcg gcgttaaagc agtaataaaa aggccagtca gtaatgttac
    64381 tgcctggcct ttaatcaatg aatcagaaac gcttacagcg ccatcaactt gtccagcgac
    64441 ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc atcacgtttg
    64501 ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag
    64561 taggcgcaga agtacagacc gtgagtgcca ctggcggtgc cgtacggcag gctctggcga
    64621 acaatcttca gccctttgcc atcttctttc agatcaacgc gggtgaggtg agaggtttcc
    64681 ggacgttcgt cgccgtcaat ctcttcgttg gcctctttgg tgcgcccgat catcatctcc
    64741 tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac
    64801 acatagctgc cgccggaatc cacgccgtct ttgataaccg ccacttcgcg gcgcgtctct
    64861 tcacccgccg ggttttctgt accgtcaaca aagccgctca ggtcacgctc ttccacccaa
    64921 cggaagccgt ggatctcttc tttcacttca atgcagtcac caaaggcttc catcgccgcc
    64981 tgagcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac
    65041 tgggtcgtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc ttctgcccca
    65101 acgccgccgc tcagagcgcg ccaggtgttg ttaccaaagg caaccaccgc accaagatgc
    65161 gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt actggccgca
    65221 cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt
    65281 tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa gaatgccacg
    65341 caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcgacggcg gtttgttcag
    65401 acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg
    65461 aaggcattaa cccttcagga ccacgccatt ccccgctgaa ctggtagcta atatgttgac
    65521 tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt tggcaattgc
    65581 caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag
    65641 gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca
    65701 tcgccacgtt gtcgcctttc atcacttcaa aaaagcgcac cacgttgccg ttcgtggttt
    65761 tcattccgct gcgcaggcga taatcgccat caagcgcatc agcaatggct tgttcttgca
    65821 gtggtgtgga cgccgttaat tcacccacgc cctgctcgct cactttggtg gacgatccaa
    65881 accagttcca cgggttagcg gcagaccagt taactgacga catcgtggaa cagccggtca
    65941 gcatcaacgg catagcgcat aacattaaac gcagcgattt catgtcactt cctttggtta
    66001 ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct ttttcttcat
    66061 taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca atatccgcca
    66121 ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc cacggttgcc
    66181 agcaaagtaa aaccacctgc gccaacagca acagaaaata gagcacatgc caggtgcgag
    66241 gaaacgaagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaata ccgggaatca
    66301 gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca gtaccctgct
    66361 cacgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa
    66421 gccagaagag caagggtaaa cgcaggcgtc cgtgcgcatc ataatggaca ggatggaact
    66481 cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc gtgttcatat
    66541 cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacgtt gatctgaatt
    66601 ttcgggcagc cgcgagcaat cagctttttc tccagccggt taagcaacgc attggcaatt
    66661 ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg atgcccgtca
    66721 taaccgccca tcaccgttcc gaccacttca ccgtttacct cagcgaccaa aaacaaactg
    66781 acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg acgcagcaag
    66841 tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc
    66901 tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa
    66961 ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt tgtcaactat
    67021 tgaaatgaaa agtaaaacaa ttctcaacag caaaccgtcg taacggatta cgcgatacga
    67081 tataacatct ggaactttat tattacaact caggccgtat gagcactttt aaaccactaa
    67141 aaacactcac ttcgcgccgc caggtgctga aagccggttt agctgccctg acgttgtcag
    67201 gaatgtcgca agccattgcc aaagacgaac ctttaaaaac cagcaacgga cacagcaagc
    67261 cgaaagccaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa
    67321 ttgataccgg agcgatcgga cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg
    67381 ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta acgcgttctg
    67441 gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc
    67501 tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt gcttcggtat
    67561 ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa
    67621 accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag
    67681 tactgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctt ggctcgcata
    67741 ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt
    67801 ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca
    67861 acccggaaga agagcggctg ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga
    67921 ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca cattcgaaaa
    67981 agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg
    68041 atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa gatagttggc
    68101 agcgcgaagc tggcggcggc gggcgcagtc gggtgttgcg taatggtggt gttttcgaac
    68161 aggcgggcgt caacttttct cacgtccacg gtgaggcgat gcctgcttcc gccaccgctc
    68221 atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc
    68281 cgcataaccc gtatgttccc accagccacg cgaatgtgcg tttttttatt gccgaaaaac
    68341 cgggtgcaga gcccgtttgg tggtttggcg gcggcttcga tttaacccct ttctatggtt
    68401 ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgccag ccatttggtg
    68461 aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc aaacatcgca
    68521 acgaacagcg cggcattggc gggctgttct ttgatgatct gaacacgcca gatttcgacc
    68581 actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat ttaccaattg
    68641 tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag ctctaccgtc
    68701 gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cacgctgttt ggcctgcaaa
    68761 ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc tgggagtatg
    68821 attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt aaggtcaggg
    68881 attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc aacgacggct
    68941 tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag aatccccact
    69001 gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta
    69061 cgacgttcag gcgaatgcgt ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa
    69121 acgcgttttg tagcgtgcgg cgactgacat gcagttgatt acacaaatcc agcaccgtca
    69181 ccggttcgga catgttttcc agcacatatt cacgggcgcg ggaaagcaac cgacggtaac
    69241 tctgatgact gatgctttcc gccgtcacca ttggttgcgc ttcttccagc atcgccccca
    69301 tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg agattttccg
    69361 ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct
    69421 gctctttcac ttccagcgcc gactggctac gcagcatatg taatacccga tccggattgt
    69481 gcaaaaagtt agcctgacag gtgatgacat cttcagacag cactacgccc aggatcgtat
    69541 aatcgtccgg cgtacttaat tcaaactcgg ttccaccggg gcgcgtcgct atttctgcgc
    69601 ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga atgccaaacc
    69661 agaacgagtt cggccagacc aggcacgact gacgcagcgc cagaccggtg tattcacgaa
    69721 aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg cccggatgca
    69781 gctgatcgta aatctgctgc taggcggtaa tcgtcaaagc atgttcatag acatccgttg
    69841 tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg ttttcgggta
    69901 agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc
    69961 cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga cgataacgac
    70021 tactgcgttt acgtaacgtc ccggcagaaa agagctgttc gagcgcattt ctggcttttt
    70081 ccagcggcca gccaaagtgg gcggccactt ctcctgccgt cattccctga catactgatg
    70141 ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg tcgggttcct
    70201 ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactgggtg tcatcgtctg
    70261 gccgaccgat ctccttacgg ctgatgacac gcccggtacg catcgcggca gcacaacctg
    70321 cgtccagcgc cgcacgacac gccgccagat cgccttccac caccagcgtt aagcgaccag
    70381 ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg gcatccgctg
    70441 catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg atcatcggca
    70501 acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca tcggtaaagg
    70561 cgttacaggc tgctttacac gcggcctggc taccggttaa aaatgctgcc gagtagttgg
    70621 tttcagacgg tggcgggacg taggtcacca gctgtacgtc ggcagatttc aacgcggcat
    70681 caatgccgta ggtcgcttcc agcggcggtg ccaccagata cgccatcgga tcgccaagcg
    70741 tgatcccggc ggttgatgaa agataagaac cggtacgcga aaccacatgc gccaggaagg
    70801 cggtgttttc tgcgtcattg gcccactgga aagctgcgcc attttcaata tgtgcaacca
    70861 tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata atcagcactt
    70921 caccggcagt cggtgacggg ccgtgtgccg ctcccgcata cagcgagcga ccatatacca
    70981 cttcgaccat cgcttgtttg gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat
    71041 cggcagaaat cagcccgagg ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt
    71101 cagcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc aaatctaaag
    71161 ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc tccagcatac
    71221 gtttcgccaa atccacaatg acggcacagc agcacgaaag agaagcaaaa gtcagctacc
    71281 tggtacacta ggcagcgggt cagcaaaggg gggcaaatgc cccttttttc atgtcagata
    71341 atcagatcat tacctcaata aactaataat cacatcgcgg ttacaaccag tttgatagtg
    71401 atagctcaaa cccagacgtg gcgctggctg caagcaaaat aagccgcaaa aatgcgaccc
    71461 catgtttact agccgcaaaa atgcggtcct atttgccaga accttttatt attcaattgg
    71521 ttagtgcaag aaaaagcgtg attactgttc aagaagtatc cagaaaggtc atgattttga
    71581 cgctgaaaaa aaataggtgg gcatttttgc ccactcacta gccgcaaatt tgcccactca
    71641 ctagccgcat ttttgcggcc caaagtaata aatacagaga aataataaat acagatcagc
    71701 gccaagattt ttcgtctttt cggcggaatc atgagactag tgcttacgtc ctgataacct
    71761 ccagagcaga ccttaacttc tgtgcagcag gactacgctc attgccgctc ggctaggtca
    71821 aatcctcacg ataaagctgt tcggattgtg accataatgt taaaggactc tttaaatcat
    71881 tccttgacga attaactagc tggcttaagc ggaatagcat gcaggataaa aacaaaacca
    71941 gcttagcagt ggggagagct tcgcttcgct cgtctcctgg ggccaatgct caatgaaacg
    72001 acgtaaggca cggagcgctc accataggta gacacataaa catctgagag cgcaccttct
    72061 atcgcgctac gcgctcaata aaaaccaacg acaccgccac actgcaagac caatggtttt
    72121 tatcgcgtat acagaccaga caggaaacag cagcaaagca gcgtatagcc aggtgcagac
    72181 agcacactca cacgacgcac agcaggctta agtgatcact caggaaactt tatccccgcc
    72241 gccgatcacc gcgaactatc atgaccgcgc caaattcgaa aggaatggcc catagaacgc
    72301 gatgaacaca cataagcgct acacttactt aacgtcgcca cagagcgatt gagagcgctt
    72361 aaaatcgatg cgatttcatt aaatttttct cactcgcctg aatggtggcg atcaggactg
    72421 atcgcaacat tgccccgcct cgatattgag cgcataatag atacatacat taagcggaaa
    72481 agagataagt ttattgtgaa aatcaggggg ttacatatca tgcgcatatc agaacaacaa
    72541 cggcagatcc tcgactatct ggcaaaaaac gaatcaatca ccgcacgaca agccgcagat
    72601 ctcgtttatg gtggcaatgt tacccgcgtc caggttgacg ccgcccgccg ctcactcatg
    72661 acgatggtta gtaatgggct aatcaggaaa caaggccgcg ctttcgtagc tgaaagacat
    72721 atcaatcacg cttatagcca ggacgttgaa gaattgcgcc gccagatcct ttgggtattc
    72781 agcaaacagg gtcaggagta tttcctttct gaacacccaa atttcgccgc caaagagtta
    72841 acagtaagcg gattgtcgtg cgcactggat tatcttgtcg gtgaaggttg cgttacttac
    72901 aagcagctat ggcacgccct gaatacgctt gtagaagatg gtttactcat tgccacgcgt
    72961 gagaacatcg aatactggat aatgccgcgt tcaggtcgcc gcaatcatgc acggtatatt
    73021 tcaggcaaca gatacagaac gccggacttt aatccgacac aggaagatcg ggaccgtgcc
    73081 gaaatgatgt gcagggaaca ggagaaaaga attatggata agttattttc gatgcagtga
    73141 acacaagcgg gcgcaatgcc cgtttttatt gccttattcc acgcctgacg aatcaatttg
    73201 atcacacctg agaaaatgca tagccagaag ttaaggccgt tatattgcga ttgggagcgt
    73261 tttaaacggt atcaatttaa ttaattctga tgtggtgatt tgcaggcaaa aaaaacccgc
    73321 cggagaagcg ggaaatagag acatatcgtt gtagttatta acagatcact ttgaggtcga
    73381 gggcggctaa ccctcgctaa tattatcccg ctattacctt gcgggtgcaa ataatctttt
    73441 attgctttga tgatttaata aatagcactt tttgctaaca aaaaccgctg tcaatatatg
    73501 gccaaaaatg tcaacagaaa aaataaggtt tataatatta agtatcacat gggtaatggg
    73561 ttcagggaat cggaatccac cgcctcacgg aaattcaaaa tttgcttctt ctttcctccg
    73621 tgggcggctt ctttttcgta tcgaggtatt tatgaaagca ataacattag aacagttcgc
    73681 agcaatggcg aatgtatcga ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg
    73741 agtccctttg ccatttaccc cacgcggtac tcgccaatta ttctgggagc acgaatgcaa
    73801 agaatggttg tacagaacag aacgataata atcacgcatc acattttgag gtaaacatca
    73861 tgaccaatat cgaaagatta gaacgctgtg ccgatattat gcgacgcaga tggatttatg
    73921 accctgaagc gggtttattg tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga
    73981 ccaataaagg gcatttggtt gtaacggtac atgaaggtaa attcaaagtg acactcacct
    74041 atcagaaagc ctgctatgta tacacatacg gcgcttatga cgagacatta tatgaagtgc
    74101 accacgttaa tttgaacaaa caggataacc gcatcaagaa tattcgcctg atgccgaaag
    74161 aaaagcaccg ccggatacat aaaaacgtcc gcaaacttat tcggctgggc cacacacaat
    74221 ggcttcaacc gcaattaatt gaacaatcac tataaacaca ggagtaaata ccatgcattc
    74281 attagacagt tattttcagc gcacgacagc ccctaaatca gccgcccagg aacgccgtga
    74341 ggaattccat gagaaagtga tgcgttctgc tgattatatt gcggacaagt tcgtggaaac
    74401 ggttcgtcca cttgttgatg aagtagctga taaacttcag tctgaaatgc cggaagatat
    74461 ggaaggcact gcaaagcggc gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat
    74521 aagcgctttt aaataatcga aaaaaggggg gaaagataat ggacgcattt gaggtactgg
    74581 caaataaagg cgctgaactg gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg
    74641 cactgctaaa taaacagatt aaagcgatcc gtatggccca gtttaaatta atcagtaatg
    74701 aagttatcga aacaattaaa ccgcagatcc cacaaatcgt tgctgacgct attaaagccg
    74761 ccggactgga gaaacgcatt gccgggctaa aaacgaacgc agagaaaatg gagatgtaca
    74821 aacagagcgg tgctccgaat cccgatgagt acttctcacc ggatgttgag tttatggcgc
    74881 aggttgagga acgtctgggt agctgtctca ccgaggaaca gcgtcgctat tttgatggag
    74941 tggatagttc ggcaggtatt gacctgaact cgtatttcgg tcgtgaaatt gagcattttg
    75001 atgccgcccc gtctatgccg gagccagaac cagaaatggc caccgatcct gaagctgagc
    75061 aacggaaacg cgtattcaat gcgataggga ttcggtactg atcggataaa agaaagccgg
    75121 gaattacccc ggctgactta acaatgtgac caatgaacaa atcggacaag acaaacagga
    75181 taagcagtct cgaaacaatg ttcgggaact atattagcac aagtccggag aaaatcatca
    75241 tgttggcact acgcatcatg cagggcatcg ctaagaccct cgcagaacat gtcttagacc
    75301 tgaaacattc gccactgagc aaacaggcca tgaagcgcca aacgcttcgt ctgtgggctg
    75361 aatattcgct tggaactatc aataaaatca tcgacatgaa atcggggccg agcaaccaga
    75421 gcgcggagga aatggagttc atcaggcgtt taattctcat tcgcagagac attcatagcc
    75481 agttacacag cgtgggaatc gatattaatg atgggacggg cgattaattt tcggacaagt
    75541 actatgcgcg cgcgaggagc tgaacatgaa gcaagatatt gcagacaggc tggagatact
    75601 cgaaggccag cgggcagaag cgaaacaact acgtaagcag gccagaagag cgcacaggaa
    75661 caacgaagca gagttactaa ccaaatacat cagtttcacc aattactgca tctacgagtg
    75721 ttacaaggaa gatgcagagg actggctgga ttcattgcca gaacagtact gagcagaata
    75781 acaccacgtc atgttgttct gaggtgagca tatcacctct gttcaggtgg ccaaatttag
    75841 tgtgccactt cagcatcacc gttgaaatca caataacgat ttttttgtaa atatgctctc
    75901 acatatgtag gaatagacca ggttggcatt gaaacacatc taccatgacc ataataaagt
    75961 aattttttga tatgtttatg gttttatatt aattaaaata tatctaaatt tgatagtttt
    76021 aaaattgcaa aatccagatt gtgtttgtaa tgtttaatct gattgtattg agtggcgttt
    76081 attatgaaaa ataaatcagg taattcttgt tatagagaag cagtcgatat tcatagctca
    76141 cttgagcttt catttatatt gcataaatat catttgcgtg tagatgagaa gaaatctcga
    76201 aatgtattca gcgccatacg tgatgagctc atccttgatg gggattcacg acaaaactta
    76261 gctactttct gtcaaacatg gcttgatgat gaaatccgtg agttgcttaa tttagctatt
    76321 gataaaaaca tgatttataa agatgaatat ccacaaatgg cggaacctga aggtcgctgt
    76381 gtgcgtatgc tttccgattc gtggagtttc cccgattcgc gacacacctt aggttgctca
    76441 accattggtt catctgaagc tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt
    76501 aaaaaacgcg aagtacaagg taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg
    76561 ctggcataaa ttctgccgtt actgtgatgt gaaattgtgt gaaattccac ttgatgatca
    76621 tcacctgata atgaatgcta aagaggtttt gaagcatgtc gatgaaaaca ctattggtgt
    76681 cgttacgact ttaggtatta cttatttttg taaagcagat tgcgttagtg tttttaagat
    76741 aagtgttaaa gttaatatta gtttttatat ttacatcgta attatatgtt tctattttaa
    76801 aaatgttcga gagtataaaa atgtctaatg ctaatatcgc acccaaaagt aagaaaatga
    76861 cagtggggac actcgctatc atgaatataa ctgctgtagt aagtctacga tttatacctt
    76921 ctgaagcaga atacggctta ggtgccattt tttactatac ctttgctgca attatgtttt
    76981 tagtacctgt tgcctttgtt gcggctgagc tagctacaac atatccacaa aaaggggggg
    77041 catttcgatg gataggagaa gcatttgggg ctcgctgggg ttttgtagga atgcttatga
    77101 cctggctaca agtcatcccc tactttccaa cagtactgac atttggtgca gtctcggttg
    77161 catttattga tgttgatatg ggggtggcag aatcaattgc cagtaataaa tggtatattt
    77221 tatttttcgt actatttgtt tactggggag ctatttgtgt tgcattacgt ggcgttggta
    77281 ttttctcgca ggtatctaag tggtgcggta ttatagggac aattattcca gctattatcc
    77341 ttgttattct tggtttctcc tatctgtggt tcagcggtaa accagcggtg atcccattag
    77401 gttggggtga tcttttccct aatatagcta acttccagaa tattgttctt gccgccagca
    77461 tatttcttgc ttatgcaggg atggaaatga atgcggtcca tgttaacgat ttagataatc
    77521 caactaaaaa atacccaatt gcaattacca tcgcttctat aggtactatt gcaatattcc
    77581 tgctcagtac tttaggggtc gcctttatta ttcctacaga taaaattagt ctaactcaaa
    77641 gtttgttggt tgcctacgat atgctgttcg aatgggctgg agtgccatgg cttggctctg
    77701 tgatggcatt tatgctagca attggcgtac tgggtggggt ggttacttgg attgcagggc
    77761 ctaatacagg tgtattagcg atcgcgaaag ctggttattt acctaagttt ttccaaaaaa
    77821 ccaatcgtca tggaatgggg caccatctaa tgtttgtaca aggtataatt gtctcagttc
    77881 tttctgttac ttttgtgata atgccttcag ttcaagcagc ttttcagatt ttatcacagt
    77941 taacagttat gctctattta gttatgtata tgctcatgtt cgctagtgct atctatctac
    78001 gacattctca acctgataca aaacgcccat atcgagcccc tgctttattt ttctgggcaa
    78061 cacttggatt cttagggtct tttctggctt ttgtattaag ttttattccg cccgagcaaa
    78121 tcccaactgg tagtccaacc agctatgttc tttatttgtt agtattagtt gccctatttt
    78181 tattaattcc aatattaatt tacgccttcc gaaaatctga ctggaaagat ccaaactctg
    78241 atttcgaacc attttcttgg gaaaaaagaa ctagtaatta aattttccat aagctacaaa
    78301 agatgaggtt aaatatgttt agtgctgaat ctccaattgg tgtaatagac tcaggtgtcg
    78361 gtggattgac atcggtaaag gaaataatta atttgcttcc tggtgaagat attatctatt
    78421 gtggagataa tatgaatgca ccttatggca accgttctgc cgatgatatc atcagtttga
    78481 ctaaaaaaat gctgactttt ttacagtcaa ggaatgtcaa actagttgct gtcgcatgta
    78541 atactatatc ctcaactttg gaatccgaag aatacagcgg atatgctaag agctttccgt
    78601 ttcctatctt atccatcatt gaacctgcag tagaagatgt gattcgccag caatataaaa
    78661 atgttggtat tatcgctacc gagtttacta ttaaaacggg gtgtcacaaa gagctaataa
    78721 aaaaaattga attcaacaat aaatgtattt ggagaaccaa gcaaaaatct tgcaatgtta
    78781 attgaggaag gtaatcttaa tgctcctgca atcttgaatg acatcaaaaa acatgttaac
    78841 catttaattt ccctccatcc agtaaatgaa attattctcg gctgtactca ctatccaatt
    78901 gttcagaatt tttttgaagc agttgcaccg gatataaaat ttattaatcc tgcacatgat
    78961 caggctatct ctattaaaaa tcacttggga cagctaaatt tattaaactc ctcaaatata
    79021 ggaactttgc aaataaatac tagtggaagt atggagatct acaaaacggt actaggggaa
    79081 ttgagcatta cgaaacctca tacattcagt atcaggcagt tttaaattta tccggtacac
    79141 cgtaaaatcc cgtccttctg aactgcaccc caaaagttgg acaccaactg agtaaggtgc
    79201 attttatgga caaacccagt tacatactgg gtagtgcatc caattagtag aacatgtgtt
    79261 tttcgataaa cgctccgatc actttttcgt ggatctcaac tgagcgtgag aagcagattg
    79321 ttttacgacc caaccgctta atgcgggtgc gtagcgtcag attattacgc tcaatgcgtt
    79381 gggtgaatat tttgccggtc agatgcttat tcttcggcac ctcccggcca tagctgcccc
    79441 agtcatcgct ggtcagcatg ccgatgttga agggtgtaag cagtgccagt agctcccggc
    79501 acgtttgatc ggttcgggga ccaaaagtgt aggccagtac accgcctgtt ttggtgttgt
    79561 acgcgtaccc gagccaatgt tgccgggctt tactgccaac gtagctccat tgctcatcaa
    79621 gctcgcagat aaacgccaca tcagcatggg caacaggcga agaagttatt cgctttggcg
    79681 cgagtttttt aaagtccgga tgacggtgtt actaccaatt ttcagtgtcc tggcggtatc
    79741 gcgaaccccg gcaccattaa aggccatttc agttatcagc tctttcatac ccggcttacg
    79801 tgcttgataa gtataagtga actgaaacac acggtggcag tcacggcagc gaagtctgtc
    79861 acggccttta gggttctgac catggcggta aacctgagct gactgacaac ggggacaatg
    79921 aatattaacg ctggtcatga gataacctca aaagcccgta ttatacatca gattcaacta
    79981 attagaggca tcacccatcc agcattataa aacatactaa caataactaa attataataa
    80041 tcacaactta caatcaaata ataaatgcat aagattaaca atgatttttt cgggttatat
    80101 ttatctaaaa aagcagaata ttatgtaact tattattttc taagcgaaaa tttattgtgc
    80161 ggtcagacta acaaacttgc gtaccacccg aagaacatgc gaagatttaa gtattctttc
    80221 ttttttggtt tttatttttc attttctctt ttcttctctt tccggttctg ttctggtttt
    80281 ggttttttcc tttttatttt tattctttct tcagggttat gctaaaacac caaaacattt
    80341 ttcaccacaa aagcatccaa tcatcttgcc agaccaatca ccttaaccgg atacactgcc
    80401 ggatataatc ccactaacac ccagacacca agcaccatac ctgataacta ccgtattcag
    80461 gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg atccgccaaa aagaggcgta
    80521 ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt caatgactgc caacgggagt
    80581 tgttgtagct gtgggcgcaa caacatgccc agagctttac caaagtcctg cccggccacc
    80641 accagcaggg gatgcggatt cggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc
    80701 gtcagaaccg cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac gtacacgtca
    80761 gttttgggat caagatccag ctgaagcagc gcctgttgcc aggcactcac cagatccgtt
    80821 tcatcaatcg ggatcgccac cggcaaattg cgcagcggca gttgtacgcc ctccagccag
    80881 attgtgctgc cagagagcga aagggtatgt gcacccgcgc caatcaccgt ggagcgtacg
    80941 gtttgcgccg gaaactgcac attcatctca cgcaggcgcg gatggtcatg cagcgccgtt
    81001 gccagaagtg ggccaatatc ggcaaaacag aacgggttgg cgggctggtg gcgataacat
    81061 tcgcccacgc ctccagaaag cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg
    81121 gtttgcatca atgcctgcgc gagcggcgaa agcgttccgt caatcacttc gacaatcagc
    81181 gccgccatcc gccgggtaac ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcagtg
    81241 cctgcaccga agcactcatc cacaatcatc tgccccggtt tatgagcgta aaccacgcgc
    81301 ccctggctgt cggtttccag caggcgacca ccgacgttga gacaggcagt gccgctgatt
    81361 tttccggcat cgaacagggc gtagttcgcg gtgccaccgc cgatatcgat attcagtacc
    81421 cgacacagcc gttgttcaga aagggtttgc gccccggctc cgtgaccggc gatcacggat
    81481 tcgaggtgcg gcccggcgct ggcgacgaca aaatcgccca gcgactgttt ttccttcatc
    81541 actttttcca tcagctgtgc gaccaggctt tcggtaaact ggccttccgg cagctgggca
    81601 atgatggttt cgcggatgcg ctggctttcg gttttgtcgg ctggcacgct ggcgggcacg
    81661 gacgccgtta ccgggatcga ttcatcacat tcggtgatgg taaaacccag tagatccgcc
    81721 acttcacggg cttccggggt aataatgctg gcgcgcagaa ccacggacat tgcctgttcg
    81781 ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga gttttttcac ctgcttcctc
    81841 ctgtttggtg agagatgcca gataatccac cagttgctgc acgctttgcg ggtcgtggct
    81901 gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc aataactttc gcgttgcggc
    81961 gacgtcagca tctggcatgt ccgttttact gatgacggcg atttgccgtt tgctgacgcc
    82021 aatatccagc aacccggcag gcaaccgact ttccgggtca tttgcgccat ggacatagat
    82081 cagcatgtct acatcctgta gcgtggtaat taaggcgtga taccagcggg gatggctaaa
    82141 atattcaccc ggcgtatcaa tatcgccctt atcattaaat tccacggcca gtgtttttct
    82201 ggcgagggta taattcccct gcaacgcatt aaataacgtt gtttttcctg ccccgacgct
    82261 acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa cttttggtca tttcacaaag
    82321 cgtataattt aataagcgac ctaaaccgct gacggtttgt gataacgctt cttctactgc
    82381 gcccaccgag ccataaatca ccaatgcacc gctgaagcgg tcgagaaaac cgatatgcac
    82441 atcagcggct ttcagcgcta aatcaccggc gatcatcgcc gtttcgccgg gagttagcgt
    82501 cataatgcca attgcgcccg catccggaac gccgattttc ttcgccagtt cctcaccggg
    82561 atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc acaaattcct gaatgatgcg
    82621 ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg tatcgtgaca atttttcgtc
    82681 agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg aggtagatca ataagaatgg
    82741 agagatattt actgttatga ataaactttc atatgcgacg catgaatata aataaaataa
    82801 tatcgagata ttttatatac tgattttttt attattcgca aactcagtaa taagctttgc
    82861 taatatattt caggaaaacc gcttaccctg aatattcagg gtaagcgtga gagttaagaa
    82921 tgaattacag cggttgggtt tgcgcttcaa ccacggccag cgccaccata ttgacgatac
    82981 gacgcaccga tgcgatcggc gttaacacgt gaaccggttt cgccacgccc atcagcaccg
    83041 gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag
    83101 cttccatgtt cggcatcacc agaatattgg cagaaccttt caaagggctg tccggcatac
    83161 ggtcgttgcg aatcgcttcc accagcgctg catcgccgtg catttcacca tcaatcatca
    83221 gatctggcgc acgatccctg accagttcca gcgcctgacg cattttgctc gacgacgggc
    83281 agtcagaaga accaaagttg gagtgcgaca acagagcaac gcgcggctca ataccaaaac
    83341 gacggacagt ttctgccgcc atcaaggtga tctccgccag ctcttctgcg tctggttcgt
    83401 cattgacgta ggtatcggca ataaaggtgt taccactcgg cagcagcagc gcattcattg
    83461 cacctgcggt gtgaacgcca tcgcgataac caaagacatt tttcaccaca ctaaaatgtt
    83521 catgataatc gcccaccgta ccgcagatca ttgcatcggc ttccccacgc tgaaccatga
    83581 tcgcgccgat cactgtcggg ttactgatca gcgcacgctg cgcctgttcc tgagtgacac
    83641 cgcgacgctt catgatctgg aagtattcgg tccagtactc tttaaagcgc ggatcggatt
    83701 cgttattgac gatttcaaaa tcaacgcccg ctttgatctg caagcccagt ttctgaatgc
    83761 gcatttcgat cacgttcgga cgaccgataa ggatcggttt cgccagtccc agcgttacca
    83821 gttcctgagt cgcatgcaga acgcgcgcct cttccccttc cggcagaact acgcgcttcg
    83881 gcgctttgcg agcctgggag aaaatcggct tcataaacag gttggttttg taaacgaact
    83941 cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg acgcgtcgcc acgcccgact
    84001 ccatcgccgc ttttgcgacc gcaggagcga tcttaacgat caagcgcgga tcaaacggtt
    84061 ttggaatgat gtattccgga ccaaagctca gatcctgaac gccatacgct gaagccacca
    84121 cttcactctg ttctgcatgg gccagttctg caatcgcgcg taccgccgcc agtttcatct
    84181 cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg gaagatgaac gggaagcaaa
    84241 gaacgttgtt cacctggttc gggtagtcgg aacggccggt acagatgatg gcatccggac
    84301 gcacttcttt cgccagcggc ggcagaattt ccggttccgg gttcgccagc gccaggatca
    84361 ttggtgcccg agccattttc ttcaccattt cctgggtcag cactttcggg ccggaacagc
    84421 ccaggaaaat atccgcacct tcaatcacat catcgagggt acgtttgccg tcatccacca
    84481 ccgcatatgc ggctttggtt tccgccatat ttggctcacg gccctgatag ataacgcctt
    84541 ttgaatcgca aaccacgatg ttatgttttt gcagccccag cgctaccagc aggttcatac
    84601 aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg cacgtcggag atgtttttct
    84661 ccaccacgcg caagccgttg aggatggcgg cagtgctgat gattgccgtg ccgtgctgat
    84721 cgtcgtggaa taccggaata ttcatccgct cgcgcagttt ctgttcaata tagaaacatt
    84781 ctggcgcttt aatgtcttcg aggttgatgc cgccgaaggt cggttcgagc gcggcgacga
    84841 cttcaataaa tttgtccggg tcgagttcat caacttcaat gtcaaataca tcaatcccag
    84901 cgaatttctt aaatagaacg cccttgcctt ccatcaccgg tttgcctgcc agcgcgccaa
    84961 tgttgcctaa ccccagcacc gccgtaccgt tagagatcac cgcaaccagg ttaccgcgcg
    85021 cggtatattt gtaggctttt agcgggtctt tttcgatttc aagacaaggt gcggcaacgc
    85081 ctggtgagta ggccagcgcc agatcgcgct gcgttgccag aggcttggtt ggagaaacct
    85141 ggattttccc tggaactgga aattcatgga aatcaagtgc actttgtttt aactggtcat
    85201 ccatttggtt gttcctttca cgtaacgttc acaaataaag tgtgtgggca acagcccctg
    85261 cccacaacgt ggcgcacatt attaccctgc cggagtctac agactttgag caagtccaaa
    85321 ctctcaccat taatataatg ttttggtaat aatcctataa cactgatgtt acctgcttaa
    85381 tccagcaata ccatgcctgt ctgctatgct tttttgatgc gtttagcgaa atttctcaga
    85441 agtgtgaatt aacgcactca tctaacactt tacttttcaa ggagtatttc ctatgaacga
    85501 gttagacggc atcaaacagt tcaccactgt cgtggcagac agctgcgata ttgagtccat
    85561 tcgccattat catccccagg atgccaccac taatccttcg ctgttactca aggctgccgg
    85621 attatcacaa tatgagcatt taatagacga tgctatcgcc tggggtaaaa aaaatggcaa
    85681 gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg gcggtcaatt tcggtgctga
    85741 aattctcaaa atcgtacccg gttgcgtgtc aacagaagtt gatgcacgcc tctcttttga
    85801 taaagaaaag agtattgaga aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt
    85861 tgagaaatca cgcattttga tcaaactggc ttcgacctgg gaaggaattc gcgcggccga
    85921 agagctggaa aaagaaggta ttaactgcaa cctgacgctg ctgttttctt ttacccaggc
    85981 gcgggcctgt gcggaagcag gcgtttttct gatttcgccg tttgtagggc gtatttatga
    86041 ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg gaagaagatc cgggcgttaa
    86101 atcggtgcgc aatatctacg actactataa gcaacaccac tatgaaacca ttgtgatggg
    86161 cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc ggctgcgatc gactgactat
    86221 cgcaccgaat ttgctgaagg agctgcagga aaaagtttct ccagtggtac gtaaattaat
    86281 cccgccttct cagacgttcc cacgcccagc acccatgagc gaagcggagt tccgttggga
    86341 gcacaatcag gatgcgatgg cggtagaaaa actgtctgaa ggcattcgtc tgttcgctgt
    86401 tgatcaacgc aaactggaag atcttcttgc cgccaaacta taaaccagcc acggagtgtt
    86461 atatgtcccg aaaagacctt gccaatgcga ttcgcgcact cagtatggat gctgtacaaa
    86521 aagccaattc tggtcatccc ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt
    86581 ggaacgattt tcttaaacat aatcctaccg acccaacctg gtatgatcgc gaccgcttta
    86641 ttctttccaa cggtcacgcg tcgatgctgc tctacagttt gctacatctg accggttacg
    86701 acctgccgct ggaagaactg aagaacttcc gtcagttgca ttcgaaaacc ccaggccacc
    86761 cggagattgg ctatacgcca ggcgttgaaa ccaccaccgg cccgcttgga caaggtttgg
    86821 cgaacgccgt cgggctggcg atagcagagc gtacactggc ggcgcagttt aaccagccag
    86881 accatgagat cgtcgatcac ttcacctatg tgtttatggg cgacggctgc ctgatggaag
    86941 gtatttccca cgaagtctgt tcgctggcag gcacgctggg actaggcaag ctgattggtt
    87001 tttacgatca caacggtatt tccatcgacg gtgaaacaga aggctggttt accgacgata
    87061 cggcaaaacg ttttgaagcc tatcactggc atgtgatcca tgaaatcgac ggtcacgatc
    87121 cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt gaaagataag ccgtcgctga
    87181 ttatctgccg tacggtgatt ggctttggtt cgccgaataa agcaggtaag gaagaggcgc
    87241 acggcgcacc actgggggaa gaagaagtgg cgctggcacg gcaaaaactg ggctggcacc
    87301 atccgccatt tgagatccct aaagagattt atcacgcctg ggatgcccgt gaaaaaggcg
    87361 aaaaagcgca gcagagctgg aatgagaagt ttgccgccta taaaaaggct catccgcaac
    87421 tggcagaaga gtttccccga cggatgagcg gtggtttacc gaaggactgg gagaaaacga
    87481 ctcagaaata tatcaatgag ttacaggcaa atccggcgaa aatcgctacc cgtaaggctt
    87541 cgcaaaatac gcttaacgct tacgggccga tgctgcctga gttgctcggc ggttcggcgg
    87601 atctggctcc cagcaacctg accatctgga aaggttctgt ttcgctgaag gaagatccgg
    87661 cgggcaacta cattcactac ggggtgcgtg aatttggcat gaccgctatc gccaacggca
    87721 tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt cctgatgttt gttgaatacg
    87781 cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg gcagattatg gtttataccc
    87841 acgactcaat tggcctgggc gaagatggtc cgacgcacca ggctgttgag caactggcca
    87901 gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg cgatcagatg gaagcggcgg
    87961 tgggctggaa gctggcggtt gagcgccaca acggaccgac ggcactgatc ctctcaaggc
    88021 agaatctggc ccaggtggaa cgtacgccgg atcaggttaa agagattgct cgtggcggtt
    88081 atgtgctgaa agacagcggc ggtaagccag atattattct gattgccacc ggttcagaga
    88141 tggaaattac cctgcaagcg gcagagaaat tagcaggaga aggtcgcaat gtacgcgtag
    88201 tttccctgcc ctcgaccgat attttcgacg cccaggatga ggaatatcgg gagtcggtgt
    88261 tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg tattgccgat tactggtaca
    88321 agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg ttacggggaa tctgctccgg
    88381 cggataagct gttcccgttc tttggcttta ccgccgagaa tattgtggca aaagcgcata
    88441 aggtgctggg agtgaaaggt gcctgatggt gatcgccgga tgctgtatgt tcccctcacc
    88501 ctaaccctct ccccagaagg gcgaggggac ggtccgtgcg ctttttaggg ccgcatctgc
    88561 gattttgttt aacgggtgat ccacaacgtg ggcctggcgt ctggcccatg ccagttatcg
    88621 caggtaggtt ctgcggcgta acgcaccagg cgaaaacgct gaccgtcaaa gcgccagcgc
    88681 gcctgaatgc cacaatcgct taatccgcgc cctttcgcta aggtcaccag ttcacgcgat
    88741 ttctcatcaa atgttgcgtt catcagttcc agttcattcg tctcctggcc gttgttgaac
    88801 ggcaaacgca accgaaccgg gcgcgaggct agcggctttt tacgcgacac aatccatgcc
    88861 aaatcaatgg tgttataggc ccctgcctca cagctaatca tcatcagtgc tttatcatcg
    88921 gtcagcgcag tgacattcac ctcacgacgt aacggatcaa gcgagcagcg cagaccattc
    88981 atccgccagt ttccataatc cagcaaatcg ttacgttctt cgagtgagag tggcgtcggc
    89041 gttgggttaa ccaccgcgac ctctttcagc gcaggcgcgg gcggtacgct gagaggcggt
    89101 tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc gcttttgctg agcatcaata
    89161 aacaacaacg ccgcttttaa gccgctcaga gaaatggtct gattgccgtc gcgtaaggtg
    89221 atcgccttcc cttcctgaat tatctgcaaa aacgcggtga tggttgccgt atcatcggtt
    89281 actaataacc atggtgaaat ccgccacttg tcaccactta atgccaacgg ctcaccatct
    89341 aatagcagcc gtggcgctat ctccccttca gacgcatccg gcgacttcaa cccaccgcgc
    89401 tcaatacgta aaacggcatc ggtatgcgcc ccagcgctgc ggctcagggt catcaccagt
    89461 ccattatgat cgcccgtatt gcgcgccaca caaaaatttt gattattaca ggtgacctgc
    89521 cagtcggaaa acgcccgttg cgctggtgcc gcccacacca aagacgtcgg caacaaagcg
    89581 aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta cgaccccaga agttcaaaca
    89641 aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc tcaatcggat ttgtcggatt
    89701 gagccaataa taaacaacga aatattattg ctatttttca gtccattaaa tgtgacattt
    89761 gcaaatagtt aagcaataac accgtcttac cgtcacgtat ctcgccggtt ttgatcatct
    89821 ccagcgcctg gctgaacggc agctcgagca cttcaatatc ttcatcttca acaccgccac
    89881 cggcgttagc gcgctgattg tcactgtatt cggcgataaa aaagtggatt agctcagtca
    89941 caccgccagg cgacatatac agttcaaata atttgcgcac ttcgcccact tcatagcccg
    90001 tctcttcaat cgcttcttta cgaatgcaca cttccggttc gtcgttatcc agcagcccgg
    90061 cgcaggtttc aatcagctgc ccgctttcat tgccattaac ccaggtagcg acacggaact
    90121 gacgaatcag aaccacgctc tttttcttcg cgttgtacag gaggatcgtc gcaccattac
    90181 cgcgatcgta aacttcacgt ttatggcgga taacttcgcc atctttgcgc gtgagatcgt
    90241 aagtaatgtt gtgcagggtg aaatagttat cggagagaat tttgtcttta atgagggtga
    90301 tttgttgcgt cataccgact ccacagcgcg aaatgaacaa ttatattacg ctgtgaagtc
    90361 gggtttgtct gcgcaggcta tcagtgagat ttgactgatt ttacccccag ccagtcaata
    90421 atcccctgtg ccgcatgacg tccttctgcc attgcggtaa ccaccagatc cgcaccacgc
    90481 acggcgtcgc caccggcgaa gattttcgga ttggtggtct ggtaacggta ctggctttcc
    90541 acatccgcga tgatgcggcc ccatttgtct accgttacac cgtgcgactc cagccacggc
    90601 atcccgtgtg gattgaagcc aaacgccata atcaccgcat cggctggcat gacaaattca
    90661 ctgccctcaa tcggtacagg tcgccgacgc ccctgggcat ccggctcgcc aagacgcgtg
    90721 cgcaggaaac gaattccgca aacgtgacct tgctcattca gctcaagcgc caccggctgg
    90781 acgttaaatt cgaagttggc cccctcttcg cgggcgttct tcacctcttt cttcgagcct
    90841 ggcatgttag cttcatcacg acgataagcg caggtgacgt tactcgcgcc gtggcgcagc
    90901 gcagtacgca cacagtccat cgcggtgtcg ccgccgccca gtaccacgac gttaagtccg
    90961 gcggtattga taaacggctc ttccggtagc tcttcgagcc ccatcacctg tttagtgttg
    91021 gcaatgagga acggcagcgc gtcataaacg cccggcgcat cttcattggg taaacccgct
    91081 ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt cgtattgttc caaaagcaaa
    91141 tccaaagaga catctttacc cacttcacaa ttgagttcga agtgaatccc catcgcgctg
    91201 aagatttccc gacggcgtgc cagcagggat ttatccagtt tgaaagaagg aatgccgaaa
    91261 gtgagcaagc caccgatttc tggatggcga tcgtacaccg tcaccgccac gccattgcgg
    91321 gtcagaacat ccgcacaggc cagccctgcc ggacctgccc cgataatcgc cacccgcttg
    91381 tccactttgg tgacatgact taagtcagga cgccaacctt tcgccaacgc ctgatctgaa
    91441 atatagcgtt caatgttgcc gatagttacc gcgccgtgct catcgcgaat agtacaggca
    91501 ccttcacaca aacggtcttg cggacaaacg cgtccggtaa tttccggcag ggtgttggtc
    91561 tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt tcaccagttc aatccactgc
    91621 ggaatatggt tatgcagcgg gcaggtccat tcacaaacgc tgtgctcgcc gaacttaagg
    91681 cagcgcgagg cttcccgttg tgcctggtcg gcgcgaaatg gcagataaat ttcatcaaaa
    91741 ccggttttgc gcgcttcaat cgccagttta tccggctcgc cacgcgcggg cgttgcctgc
    91801 atttgttcga ctttactcat taccggcatt tcttgtgcag cggtactggc atgccacggt
    91861 tgatgctcct gacgtgcggt gcgcaagcgg cgggatttcg ccatgccgga gagtgcgacg
    91921 tcagtgacca gttgcagcgc gtccgccggg caattctcga cacaggcagg accgttttcg
    91981 cgccctgcac aaaggtcgca tttatgcgcc gtggctttta cttttcctgc cgcgacgggt
    92041 gtcaggacga tttgcatcgt accaaacgga caggccacca cgcaggattt acagccaata
    92101 cacttttgct gattgacctg aatgctgtca tcaacgtggc tgattgcgcc attagggcag
    92161 ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca ctgcactacg ttgctgctga
    92221 tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg ttgctcatca
    92281 ttgtgagcca tgacacaggc gatttcacaa gcatgacaac ccagacattg ctgactgttg
    92341 gccataataa aacgattcat aacgaccttc ttttttggtt gtaaaaacct tattctttat
    92401 atgagtgttg ttattacccg acttacaggg gaaccggcaa tgttcatgtg cccagaataa
    92461 gtaactattt cgctataaac tgtggcagat caaataatcc catcactgac taaattgcgt
    92521 ttcagcgaac tggaacatta atgatttttt gtggagaaga cgcgtgtgat tgttaaacga
    92581 cccgtctcgg ccagtctggc ccgggccttt ttttacattg tgctgctgtc gattctttcc
    92641 acgggtatcg ctctgctaac tctggcgagc agtttgcgcg acgctgaggc tatcaatatt
    92701 gccggatcgc tgcgtatgca gagttaccgc ctgggctacg acttgcaaag tggcagtcca
    92761 caactcaatg cacatcgcca gttgtttcag caggcactgc attcaccggt attaaccaac
    92821 ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct atgcgcatct gaatgccaac
    92881 tggctggaga tgaataatcg gctcagcaag ggcgatttgc cgtggtatca ggccaatatt
    92941 aataattatg ttaatcagat agacctgttc gtgctggctt tacagcacta cgctgaacgc
    93001 aaaatgctgc tggtggtggc gatttccctg gctggcggta tcggtatttt cacgctggtc
    93061 ttttttactt tgcgccgcat acgccatcag gtggttgccc cgctgaatca gctggttacc
    93121 gccagtcagc gtattgaaca cgggcagttc gactcgccgc cgctggatac cagtctgccg
    93181 aatgagcttg gtctgcttgc aaaaaccttt aaccaaatgt cgagcgagct gcataaattg
    93241 tacctttcgc tggaggcgtc agtagaagaa aagacccgcg atctccacga gaccaagcgt
    93301 cgtctggagg tgttgtatca gtgcagtgtt cccaggcgct gaacaccagc cagattgatg
    93361 tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcagct gaatatctgg
    93421 agctgaacgt tggggataac tggcggatta gcgaagggaa gccaaaccct gagctgccaa
    93481 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
    93541 gtcacgtttc atcatcagaa ccgctgctta acagtgtttc atcgatgctg ggacgcggtt
    93601 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcga
    93661 ccatcgcccg cgaattgcac gactcgctgg ctcaggtgct ttcttactta cgtatccagt
    93721 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
    93781 atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg actactttcc
    93841 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
    93901 aaaatcaaac cagcgctaaa ctgaccctcg actgccgtct gccaaccctg gcactggatg
    93961 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc
    94021 acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg
    94081 tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa ggtcattatg
    94141 gactgaatat catgcgcgaa cgcgcggaac ggctgggcgg gacgctgact ttttcacaac
    94201 cttccggcgg cggcacgtta gtgagtatta gctttcgctc cgcagagggt gaggggaagg
    94261 ggaacacgcc ggctgaggtg taaataatta ccctcacacc ggccctctcc ctttaaaggc
    94321 gagagggaaa aacgtgccca tattgacctg taagcgagtg tgtcagcaat ctcatgataa
    94381 tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta cagtgaagca
    94441 agtcaagcct acaacaatac gcagaaacac gaggtcctct tttaatggcg aatttcttta
    94501 ttgatcgccc catttttgcc tgggtgctga caatcctgtt gtgtctgaca ggtaccctgg
    94561 cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat gtgcgagtga
    94621 ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc caggttatcg
    94681 agcaaaatat gaccggcctc gataatctga tgtatatgtc atctcagagc agtggcaccg
    94741 gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa gccgtgcagc
    94801 aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg caaaatcagg
    94861 gcgtgacggt gcgtaaaacc ggcgatacca acattctgac cattgccttc gtctctaccg
    94921 atggctcgat ggataaacag gatattgctg attatgttgc cagtaatatt caggacccgt
    94981 taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat tccatgcgta
    95041 tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat gtcactgatg
    95101 ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca ccttccgtcg
    95161 ataagcaggc gctcaacgcc accattaacg cccagtcact gctgcaaaca ccagaacagt
    95221 tccgcgatat caccttgcgg gtgaatcagg acggctcaga ggtaaggctg ggcgatgtcg
    95281 ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat ggtaagccag
    95341 cctccgggtt gggggtaaaa ctggcctcca gcgcaaacga aatggcgaca gcggagctgg
    95401 tgctcaatcg tctcgacgag ctggcgcagt atttcccgca cggactggaa tacaaggtgg
    95461 cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa acgctgctgg
    95521 aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc cgcgccacgc
    95581 tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta ctttacgcct
    95641 tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc ggtctgctgg
    95701 tggatgacgc catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag gaaggactca
    95761 ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg gtcgggattg
    95821 cgatggtgct ttcggcggta tttgtaccaa tggccttctt cggcggcacc accggtgcca
    95881 tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta ctggtagcga
    95941 tgctcctcac tccggctctg tgtgccacgc tacttaagcc actgaaaaaa ggtgagcatc
    96001 acgggcaaaa aggttttttt gcctggttta accagatgtt taaccgcaac gccgaacgct
    96061 acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg atttatgtcc
    96121 tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc ttaccgctgg
    96181 aagaccgggg catgtttact acctcggtac aattgcccag cggttcaacc caacaacaga
    96241 ccctgaaagt cgttgagcaa atcgagaaat attacttcac ccatgaaaaa gacaacatca
    96301 tgtcggtgtt tgccaccgtt ggttctggcc ctgggggtaa cgggcaaaac gtggcgagaa
    96361 tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc tcgtttgcca
    96421 ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt atcgccagca
    96481 gcccaccagc aattagcggt cttggtagtt ctgcaggttt tgatatggag ttgcaggacc
    96541 acgctggagc gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg gcgctggcgg
    96601 cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgatgacagt ccgcagttgc
    96661 agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgatatcgac gatattaacg
    96721 acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat cgcggtcgcg
    96781 tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat gacatcaatc
    96841 tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc gcgacctcac
    96901 gctgggaaac aggctcgccg cgtctggaac gctataacgg ttattctgcg gttgagattg
    96961 ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg gaatcgttag
    97021 tgaagcagct gccaaacggc tttggtctgg agtggacggc gatgtcgtat caggagcggc
    97081 tttccggcgc gcaggctccg gcgctgtacg ccatttcctt gctggtggta ttcctgtgtc
    97141 tggcggcatt gtatgaaagc tggtcggtac cgttctcggt aatgctggtc gtgccgctgg
    97201 gggtaatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc
    97261 aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg atcgtcgagt
    97321 ttgctaacga gatgaaccaa aaaggccatg acctgtttga agcgacgctc cacgcctgcc
    97381 gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc gtattgccaa
    97441 tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg
    97501 gcgggatgat ttcagctact attctggcta tttacttcgt gccgctgttc tttgtgctgg
    97561 tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag gcgacatgcc
    97621 aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc ttgctgttta
    97681 tactttgtct caggaattac ttacgaagca taacttcgat aaagtctttc cagttcccca
    97741 gttcacgttc aatcataaca acctctctta taattatggg tattctacgg aaacaatata
    97801 ccgtggtgaa gctaatttac tcgattgctg cgatgactac ctccggggga caaaccttat
    97861 gtaaatacta tggtcctgcg gtgataattt gtatgtgata cacagcaaca tttcgagata
    97921 ttcatacggc atctaatact gatttaattc tggttaaaat acagacagat aacaagatga
    97981 atattcttaa tgtttacgtt aaaaatgttt aatattattt aatagttgtt aatttgaata
    98041 cttcgataat gttatatttt ctgataatca tttgcaggca aaatgttttc acccttaaat
    98101 gagtatttat tctcataaat cgaaaaagga ttcattatgg ttacacttta cggcatcaaa
    98161 aattgtgaca ccattaaaaa ggctcgccgt tggctggaag ccaataacat cgactatcgt
    98221 tttcatgatt accgcgtcga tgggctggac agcgaattat tgaacggttt tatcaacgaa
    98281 ttaggctggg aagcgttact caacacccgt ggtacaacct ggcgtaaact ggacgaaact
    98341 acccgcaata aaatcaccga tgcggcctct gcggcggcat taatgactga aatgcctgca
    98401 attatcaaac gtccattgct ctgcgcgcct ggtaagccta tgctgctggg tttcagtgat
    98461 tccagttatc agcaattttt ccatgaggtg tagtctatgt cgtgcccggt tattgagctg
    98521 acacaacagc ttattcgccg cccttccctg agtcctgatg acgcaggatg tcaggctttg
    98581 ttgattgaac gtttgcaggc gatcggtttt accgttgaac gcatggattt tgccgatacg
    98641 cagaattttt gggcatggcg tgggcaaggt gaaacgttag cctttgccgg gcataccgac
    98701 gtggtgccgc ctggcgacgc cgatcgttgg atcaatccgc cgtttgaacc caccattcgt
    98761 gacggcatgt tattcgggcg cggtgcggca gatatgaaag gctcgctggc ggcgatggtg
    98821 gtagcagcag aacgttttgt cgcacaacat cccaaccata cggggcgact ggcatttctg
    98881 atcacttctg atgaagaagc cagtgcccac aacggtacgg taaaagtcgt cgaggcgtta
    98941 atggcacgta atgagcgtct cgattactgc ctggtcggcg aaccgtcgag tatcgaagtg
    99001 gtaggtgatg tggtgaaaaa tggtcgtcgc ggatcgttaa cctgcaacct caccattcat
    99061 ggcgttcagg ggcatgttgc ctacccacat ctggctgaca atccggtaca tcgcgcagca
    99121 cctttcctta atgaattagt ggctattgag tgggatcagg gcaatgaatt cttcccggcg
    99181 accagtatgc agattgccaa tattcaggcg ggaacgggca gtaacaatgt tattccgggt
    99241 gaactgtttg tgcagtttaa cttccgcttc agcaccgaac tgactgatga gatgatcaaa
    99301 gcgcaggtac ttgccctgct tgaaaaacat caactgcgct atacggtgga ttggtggctt
    99361 tccgggcagc catttttgac cgcgcgcggt aaactggtgg atgcggtcgt taacgcggtt
    99421 gagcactata atgaaattaa accgcagcta ctgaccacag gcggaacgtc cgacgggcgc
    99481 tttattgctc gcatgggggc gcaggtggtg gaactcgggc cggtcaatgc cactattcat
    99541 aaaattaatg aatgtgtgaa cgctgccgac ctgcagctac ttgcccgtat gtatcaacgt
    99601 atcatggaac agctcgtcgc gtgatgagtg gttctgcaag aggaaataag catggactgg
    99661 ctggctaaat attggtggat tctggtgatt gtctttttgg taggcgtcct gctgaacgtg
    99721 attaaagatc tcaagcgcgt cgaccataag aaatttctcg ccaacaagcc ggagcttccc
    99781 ccgcatcgtg atttcaacga taagtgggac gatgacgacg actggccgaa aaaggatcaa
    99841 ccgaagaagt aagctgagtg ctcaaccgta ggccggataa ggcgttaacg ccgcatccgg
    99901 caatggtgaa cgatccctga tgcgacgctc acgcgtctta tcaggccagg taatctgtct
    99961 tttacatcat ttcaatcaca tcatcatcgc caggcttacc gccacttagc gcttggtcga
   100021 aataatgttt cggaatggta taacgcaagt gatccagggc aaattgcata ctgcgattgt
   100081 cgatcgcatg gcctaaatcc tccacgatat ccagcgtcac gtcaccaccg gcactgatta
   100141 acgcttcctg cgccgcaact gcatgcgcca gatcgataac cggatcttca ccaccatgaa
   100201 tcaagtgaat tgtcgtggct gtagacgcgg tctccggcag gctcgcatag cgtccgttaa
   100261 aggcaatgac gcgcgaagca aggcccggtt cggctttaat gctctccagc gccataatcg
   100321 cgccttgtga gaaaccaatt agcgccgtgg cgttagcccc tactccgctc tgtttctgcc
   100381 agtagcgtac cgtttcaata aacgtcggca tgatggcatc cacacgcgcc tggcgattat
   100441 cttccgtaat accctgcact gaaaaccact gacgccccgc cggattaccg ctcggctccg
   100501 cgccaccgac actcaccacc agcgcatcag gaaacagcgg tgcaaaccag ctgcctattt
   100561 cccccattgc caccgggtta tccccgacac cgtggaaaag cagtaacaac tgttgtgcag
   100621 gtttatccgg gctttgaaca acaaaatggt catgtttcat ggcgaactcc ttaactgatg
   100681 tcattaattt tacgccgctg actcacaatg accatgggag aaaattgaag gagtcagtga
   100741 aaaaattgcc attgcgttat gcgatcgcgc agacgctcag tgcgaacctc atttagtgcg
   100801 aacagcgcct gcgcggcctc ttcccgctgg cgaaccagta acatcttgcg gcctgaaagt
   100861 ttaagtgtgg tacataactg cgcgtcactg gcgtttttct gtaaacgccc acgcagcgcc
   100921 ggtaatgcca gttcactggt ttgtagcaga cgcaataagc aacctaacga tgttaatagc
   100981 ggacgatgag cgaaagcaaa accggccagt tcaagccagt cgtcgtcaga aaggatggca
   101041 tcgtttagtg gatcaacagg aagcatttcg ccattccact gcgcgagagc ttgcgcatcg
   101101 cgacgtaaac ggtaatgctc acgttcagcc agctgtttac ccgcatcact catcggtaac
   101161 agcgccatcg ccgtatagca accgctgctg gcttcccgat gattacccat ccgcaccagc
   101221 acaaaaccgc agcgttgcca gaaacgccat aactccccag tgtaaccaaa actcaccgaa
   101281 agatagtcga ggtcatgcgt atattgcaaa gctccagcaa taagttgtcg ccctgtgcct
   101341 tcccgctgac gagccggatg aactgctatc cggctaaccc gccttccgcg caatgtcgcc
   101401 gccagtggat tgctgccgtg cgccgccagc gactgggcca ccagattacc ccgcgggcga
   101461 cgaaaacctg cccataccgc ctgactgagt tcttgagata atccgccctc atccaccagc
   101521 cacaacgccc cggcaatctc gttttcgcca gccgcctgta aaaaatgttg ccctggtgca
   101581 tccatcatcc ggcgtaaatc cagcggcgag gtccggtagt gcgcaccaga caataactga
   101641 taaaccttta acggcgtttc tggctcgctt cgccataacg tttgttcaaa tgcggaaatg
   101701 acgatattgc cttgtggtgt atgggtgaag ttttcatcgt taaaaaccag tgcctcacta
   101761 accatttttt ccagcgggca tccctgtgcc cagcggatcg gctgttgcag ttcaaaacgg
   101821 tgtaaatgcg gaaagcgagc gcaaaatttc agcaaaaaac cacgtccggt accttcgtag
   101881 ccctgcaccg tagtggttaa caacgttcga ggaaaacgcg ataccagttg atgcaacaac
   101941 ggcgcaggta tggctgcggc ttcgtcgacc accagccagt cggcttgctc atcgctggct
   102001 aacaaggcat ccggtgcaat aaagcgaaac ttctcgcccg caaattgtgc cagtacatcc
   102061 gttgccgctt ttgcgggcgc ggtgacaatc gcactgcccg caatacgaga aatgagttgc
   102121 cctgccaacg ccgacttacc gcgcccacgc gcagccgtta ccgctgccac gcccggcggc
   102181 atggtcagta gctgctgtaa gagttgctgt tgttctggtt gtggtgcgcc ggtcgcgggg
   102241 tgccagtcag tacggggagt aaaatgcgcc aacgagaacg gctggttttg ccgccagagg
   102301 atagcgtcgt tatccgccgt aagtacgcgt ttgagatgct ggacaaaatg cggcgtcgca
   102361 atagggtcag ggcaatcact ccagcgcagc gagtcggcat caggttggtt ttcccactct
   102421 tcccatacag ggagtaacaa aaccagccag cttcccgctt tcaacgttcc gctaagtgct
   102481 gcaaaggcag cggcatcaaa gccgtggcgg gcgtcgaata ccgcatgccg gaactcgcgc
   102541 ccaagtaaag tttgtagcgc cgagggtgaa cagtggtttt cagcatctag ctgcggcgaa
   102601 atccacagcc agtcgccagg taaggcatca cgcaacttaa gagcatgatc aaaacaccaa
   102661 ccctcttccc cgctcaacac cagcaagcgg cagatccctt cacgtttcat ttgcgctgtt
   102721 aatgtgtaaa gcgcagtcag ttcagccatc cctgccccgg aaattaaatg cttttaccaa
   102781 aagtattgca ttgtgccgga tcgccgctgt cgaaaccacg tttaaaccag ctgtagcgtt
   102841 gctgagaagt gccatgtgtg aaactgtctg gtactactcg cccctgactt tgctgttgta
   102901 aacggtcatc gccgatggcc tgcgccgcat tcagcgcctc ttccagatcg ccagtttcca
   102961 gaacgccttg ctgctgcata ctatgccccc agacaccggc aaaacagtcg gcctggagtt
   103021 ccatacgcac agataagcgg tttacttccg cctgcgtcgc gttttgttgc agttgacgaa
   103081 ctttcggctc gatgcctaac agtttctgca catgatgacc gacttcatgg gcgataacgt
   103141 acccctgggc aaaatcgcca tccgcgccaa gtttgtcttt catgtcatca tagaaggaga
   103201 gatcgatata aactgtgcca tccgccgggc aatagaacgg ccccataatg gactggcccg
   103261 ccccgcagcc ggtgcgcgtc attccacggt acatgaccag cttcggttgc tgataggtct
   103321 tacccatctt ttcgaactgt tgtccccagg tgtcttccgt ggttgcctga atcaccgagg
   103381 tgaattttgc ggcttcgtct tcattagggc taattgaccg cgttgattgt tgttgagaaa
   103441 ccggctgccc ggtcatcaac ccggttaaat caacgccata gtagcctgca accagcacga
   103501 ctatcagtaa aatcagcccg cctttaccgc tcggcagacg gaaaccggga ccgcccattg
   103561 acggaccgcc agagctgttg cgcctgtctt caacattgtc actttcacgt cgcccttgcc
   103621 aacgcataga tacctcaaca atatattcat tatagaaatg atcgtaggtg gttcagagga
   103681 agattaccac aggaaagcgg gatgaaatgg cggaggaatg agcgttcaat gcatatattc
   103741 cgagagcggt tgtcttgccg tgccagctgc acggcaagat gatgaacaga aaaatcagtc
   103801 cagctgtaca cccaggcggc gggcgacggc ttcataggct tcgatcaggc caccgaggct
   103861 ctggcggaaa cggtctttgt ccattttctc cagtgtttct ttgtcccaca ggcggctgcc
   103921 gtccggggag aactcatcgc ccagtaccac ttcacctttg tacagaccaa actccagctt
   103981 gaagtcgacc agaatcagac cagcatcatc gaacagtttt ttcagcacgt cgttcgcttt
   104041 gtaggtcagc tctttcatac gcgccaggtt ctctttgctc acccagccaa aggtttcgca
   104101 gtaagattcg ttgaccatcg gatcgtgcat ggcatcgttt ttcaggaaca gatcgaacag
   104161 cggcgggttc agctcaatac cttcttcgat tccaagacgt ttcactagag agccagcagc
   104221 acggttacgc acgacacact caaccggcac catatccagc tttttcacca gacattcggt
   104281 atcggagagc agacgctcca tttgagtcgg gatacccgct tcagccagtt tgctcataat
   104341 gaagtagttg aacttgttgt tcaccatacc tttgcgatca aactgctcaa tgcgcgcgcc
   104401 atcccctgct gacgtatcat tgcggaattc gagcaccaac aggtccgggt tttccgtgct
   104461 gtatacggtt ttcgctttac cacgatacaa ctcagcttgc ttttgcatct ttatcactcc
   104521 tgggtgtgaa ttaacgtttt aaaatctttt gctgtctggt gtgccggatg ttttgtcgga
   104581 tgcggcgtga acgccttatc cgaactacgt ccgacaaaca aatttcgtgc gaattaccgc
   104641 taaaatcgcg ttttcctgcc gacgcacacg tttgcgtatc atatcagaaa aaagggccgg
   104701 atgattccag ccctgtattt ttacttgcta aacgcagcct ggaagacagc taccagcgcg
   104761 tcgttctgac tctgagtcag agtatgacct ttcggttcga tgaactgtag gctgctgcgg
   104821 ttatctaaat cgccaacctg cagtttatag tcaccggatg ccaggcctgg atcgctcgcg
   104881 cccagttcct gccagtcgct gtcagacagc ggcttataag ttacggccat gttgccctgc
   104941 gaacgggtgc tgtcggtcac tttcatgccc actttttcca gcgccgctgg cagacgttgc
   105001 caaaccacat tgaacggccc gcgtacgacc agcattggta aaccggtgtc atcagctgca
   105061 ctttgtacgt ccatagtggt ggaggcacga ttttgcgcag cgttcgcggc gtcagtggca
   105121 gatttatcca gaccggcgga aataacgttc atcatctccg tgctgtaacg ctgcatggaa
   105181 gccgcgtctg caaccggttt gcccgcctgt tccaggttca gcagtttaac cgtaaccgcc
   105241 tgctgataac cctgcggctt aacagagatt tgataacgac cacgatactg ctcgtcttcg
   105301 tccagacggt tccattgtac ccaatcggtg gtcagtgtct gaccagcatc atcacgttgg
   105361 gtgatggtgt agtttttcgc ctgcagcacg ctaaccacct gcggccacag agtattgcca
   105421 cgaccatttt ccaccagcaa tgaagcggta tcgcccgtga actgggtacg cgcgccagaa
   105481 accagtgcca acggctgggc tggtggacga atgtccagcg ccttaccgac agcaccacta
   105541 ccgttggtca ccgggattgc ataatcaccg gaggtcaccg gcaaaatcat tccagccggg
   105601 gcatgaagct ccgcaagcgg tgccgcttcc aggtaggctt catcaccact gacctgacgc
   105661 ttatagcgtg agtcagaact acaggcagcg agtaataaaa caagcgaaac acccgcaacc
   105721 tttgccaggc gcgacttttg aacagagtaa gccatcaaat ctccctaaac tttacagcaa
   105781 accggcatgc ttaagcgccg ctctgaccgt ctcacgacca ctgtcggtga ttggtgtcat
   105841 tggcaggcgc agcgtatcgg tcgccacaag acccagttcc ttacatgccc atttcaccgg
   105901 gattggattg ggttcgacaa atagtttgtt gtgtaatggc atcagacgct gattaataac
   105961 gcgtgcctcg gcaaaatgcc cttctgctgc cagtttgcac atctgggcca tatcacgcgc
   106021 tgcgacgtta gccgtaacgg aaataactcc atgaccgccc aattgcatga agtccagcgc
   106081 gctcgcatca tcgccgctca gcagaacaaa atcatctgaa accagctctt tgatctggtt
   106141 tacacgcgtt aagttccctg ttgcctcttt gattccgata atatttttta ctttcgccag
   106201 acggcccacc gtttccggga gcagatcgca gccagtacgg gacggcacat tatacagaat
   106261 ttgcggcagg tcagtatgct cagcgatggc tttgaaatgc tgatacaaac cttcttgcga
   106321 cggacgattg tagtaagggg ttaccgtcag gcagccgacg ataccactgt cattgaagcg
   106381 ctgcgtcagg ctaatggctt ccgcagtagc gttagcgccg gtcccggcaa ttaccggaat
   106441 gcgcccatca gccagatcca gcgtcatcat caccacatca gcatgttcgt catgatttaa
   106501 ggtagcggac tcgccagtgg tgccaacaga aacgatcgcc gaagtaccgc tggcgacatg
   106561 ataatcaatc agttttttca agctagcccg acagacatta cctttttcat ccatcggagt
   106621 aacaatcgcg acaatacttc ccgtgaacat gggccatcct ctgtgcaaac aagtgtctca
   106681 atggtacgtt tggtatggca ttaaaagcaa gcagacagaa ccgttctgat tgttgtatgc
   106741 atgatttttt tatgctttcc ttaagaacaa ctcacccctt aaaggaataa ccagtttgac
   106801 actgtcatcg caacattatc tggtgatcac tgcgttgggt gccgatcgcc ctggaattgt
   106861 gaacaccatc acccgtcatg tcagtagttg cggctgtaat attgaagaca gtcgcctggc
   106921 gatgctggga gaagagttca cgtttatcat gctgctttcc ggttcatgga atgccatcac
   106981 tctgattgaa tcaacgttac cgttgaaagg tgccgaactg gatcttttaa tcgtgatgaa
   107041 gcgcacgacg gcgcgtccgc gtccgccaat gccagcatct gtctgggttc aggtcgatgt
   107101 tgcagactcc ccgcatttaa ttgaacgctt cacagcactt ttcgacgcgc atcatatgaa
   107161 cattgcggag ctggtgtcgc gcacacaacc tgctgaaaat gaacgggctg cgcagttgca
   107221 tattcagata accgcccaca gccccgcatc tgcggacgca gcaaatattg agcaagcgtt
   107281 caaagcccta tgtacagaac tcaatgcaca aggcagtatt aacgtcgtca attattccca
   107341 acatgatgaa caggatggag ttaagtaatg aatccactga aagccggtga tatcgcaccg
   107401 aaatttagct tgccggatca agacggagaa caagttaatt tgaccgactt ccagggacag
   107461 cgtgttctgg tttatttcta cccgaaagcc atgacccccg gctgtaccgt acaggcctgc
   107521 ggcttacgcg ataacatgga tgagttgaaa aaagcgggcg ttgatgtgct gggtatcagc
   107581 accgataaac ccgaaaaact ctcccgtttt gcggaaaaag agctgcttaa ctttacgctc
   107641 ctgtctgatg aggaccacca ggtgtgcgaa caattcggcg tctggggtga aaagtccttc
   107701 atgggcaaaa cctacgatgg tattcatcgc atcagcttcc tgattgacgc tgatggcaaa
   107761 atcgaacatg tctttgacga tttcaaaacc agcaatcacc acgacgttgt gctgaactgg
   107821 ctgaaagaac acgcctgatt actttgctcc attccgtgct ggctgcgctt gcggccagca
   107881 tacctcactt ctcgtgatca agatcacatt ctcgctttcc cctgcgacac gggtgtcgaa
   107941 tccatttttt gctgaacgtt aatgaccatc atttttgtac cgttcagaat ccagttaata
   108001 cataacttat tgaatagatt gagttaatca gaatggcatc ctttatacaa tatgaaatgc
   108061 aatgtttcat atcattttca aggagccgac atgaaccgct ttgtggtggc cgaaccactg
   108121 tggtgtacag gatgtaatac ctgtctcgct gcctgttcgg acgtgcataa aacgcaaggt
   108181 ttacagcaac acccgcgcct ggccctggcg aagacgtcaa caatcactgc ccctgtcgtg
   108241 tgtcatcact gtgaggaagc cccttgcctg caggtctgcc cggtcaatgc catctctcag
   108301 agggatgatg cgatccaact caacgaaagc ctctgtattg gctgcaagct ttgcgccgtg
   108361 gtctgcccat ttggcgcaat cagcgcttca ggaagccgtc cggtgaatgc ccatgcgcaa
   108421 tatgtttttc aggctgaagg ctcactcaaa gacggcgaag aaaacgtgcc gccccaacat
   108481 gcgttgctgc gctgggaacc gggtgtccag accgtcgcgg tgaaatgcga cctgtgtgat
   108541 ttcttgccag aaggtccggc ctgcgttcgc gcttgcccga atcaggcgtt acggctgatc
   108601 accgacgata gcctgcaacg tcagatgaaa gaaaaacagc gccttgccgc aagctggttt
   108661 gccaatggcg gggagtctcc cctttccctc actcaggagc aacactaatg gatgccctgc
   108721 aattattaac ctggtcgctg atcctctatc tgtttgccag tctggcttcg ctgtttttac
   108781 tcggtctgga cagactggct attaagcttt ccggcatcac atcgctggtg ggcggcgtga
   108841 ttggcatcat cagcggaatt acgcaattac atgctggtgt aactttagtc gcccattttg
   108901 ccaccccttt tgactttgcc gatttaaccc tgcgaatgga tagcctctcg gcatttatgg
   108961 tgctagttat ctccttgctg gtggtggttt gttcgctcta ttcattgact tatatgcgcg
   109021 aatacgaggg caaaggcgcg gcggcgatgg gcttctttat gaatcttttc atcgcatcga
   109081 tggttgccct gctggtgatg gacaacgctt tttggttcat cgtgctgttt gaaatgatgt
   109141 cgctgtcttc ctggtttctg gtcattgcca ggcaggataa aacgtcgatc aacgctggca
   109201 tgctctactt ttttatcgcc cacgccggat cggtgctgat tatgatcgcc ttcttgctga
   109261 tggggcgcga aagcggcagc ctcgattttg ccagtttcgc acgctttcac tttctccggg
   109321 gctggcgtcg gcggtgtttc tgctggcctt tttcggtttt ggcgcgaaag cggcagcctc
   109381 gattttgcca gtttcgcacg ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc
   109441 tggccttttt cggttttggc gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc
   109501 gcgctcaccc tgccgcacca tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa
   109561 tcggtatttt cggcatcctg aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc
   109621 tgtggtgggg cattctggtg atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat
   109681 atgcgctggc gg
//
LOCUS       DAJTMX010000002        95523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTMX010000002 DAJTMX010000000
VERSION     DAJTMX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.002"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO145_000109"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO145_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5465377.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO145_000110"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO145_000110"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5465378.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO145_000111"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO145_000111"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5465379.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO145_000112"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO145_000112"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5465380.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO145_000113"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO145_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5465381.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO145_000114"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO145_000114"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5465382.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO145_000115"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO145_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5465383.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO145_000116"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO145_000116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5465384.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO145_000117"
     CDS             complement(8942..9223)
                     /locus_tag="OO145_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465385.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO145_000118"
     CDS             complement(9237..10898)
                     /locus_tag="OO145_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5465386.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO145_000119"
     CDS             complement(10882..11208)
                     /locus_tag="OO145_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5465387.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO145_000120"
     CDS             complement(11264..11446)
                     /locus_tag="OO145_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465388.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO145_000121"
     CDS             complement(11430..11870)
                     /locus_tag="OO145_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5465389.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO145_000122"
     CDS             complement(11870..12166)
                     /locus_tag="OO145_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5465390.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO145_000123"
     CDS             complement(12163..12501)
                     /locus_tag="OO145_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5465391.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO145_000124"
     CDS             complement(12498..13709)
                     /locus_tag="OO145_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5465392.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO145_000125"
     CDS             complement(13711..14283)
                     /locus_tag="OO145_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5465393.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO145_000126"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO145_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OO145_000127"
     CDS             complement(15781..16005)
                     /locus_tag="OO145_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465394.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OO145_000128"
     CDS             complement(16147..16308)
                     /locus_tag="OO145_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5465395.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OO145_000129"
     CDS             complement(16318..16623)
                     /locus_tag="OO145_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465396.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OO145_000130"
     CDS             complement(16620..16898)
                     /locus_tag="OO145_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465397.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OO145_000131"
     CDS             complement(17187..18935)
                     /locus_tag="OO145_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5465398.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OO145_000132"
     CDS             complement(18932..19231)
                     /locus_tag="OO145_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5465399.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OO145_000133"
     CDS             complement(19237..19470)
                     /locus_tag="OO145_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465400.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OO145_000134"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OO145_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OO145_000135"
     CDS             complement(19685..19906)
                     /locus_tag="OO145_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465401.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OO145_000136"
     CDS             complement(19896..20096)
                     /locus_tag="OO145_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5465402.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OO145_000137"
     CDS             20226..20405
                     /locus_tag="OO145_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465403.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OO145_000138"
     CDS             20530..20859
                     /locus_tag="OO145_000138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465404.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OO145_000139"
     CDS             complement(20909..21097)
                     /locus_tag="OO145_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5465405.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OO145_000140"
     CDS             complement(21472..22701)
                     /locus_tag="OO145_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5465406.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="OO145_000141"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="OO145_000141"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5465407.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO145_000142"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO145_000142"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5465408.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="OO145_000143"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="OO145_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5465409.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="OO145_000144"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="OO145_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5465410.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="OO145_000145"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="OO145_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5465411.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="OO145_000146"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="OO145_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5465412.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO145_000147"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO145_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5465413.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO145_000148"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO145_000148"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5465414.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO145_000149"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO145_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5465415.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO145_000150"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO145_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5465416.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO145_000151"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO145_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5465417.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="OO145_000152"
     CDS             34739..35812
                     /locus_tag="OO145_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5465418.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="OO145_000153"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="OO145_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5465419.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="OO145_000154"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="OO145_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5465420.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="OO145_000155"
     CDS             complement(40574..40786)
                     /locus_tag="OO145_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5465421.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="OO145_000156"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="OO145_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5465422.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="OO145_000157"
     CDS             complement(41765..42304)
                     /locus_tag="OO145_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5465423.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO145_000158"
     CDS             complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO145_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5465424.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="OO145_000159"
     CDS             complement(44243..44785)
                     /locus_tag="OO145_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5465425.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO145_000160"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO145_000160"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5465426.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO145_000161"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO145_000161"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5465427.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO145_000162"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO145_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5465428.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47682)
                     /locus_tag="OO145_000163"
     CDS             complement(47305..47682)
                     /locus_tag="OO145_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5465429.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO145_000164"
     CDS             complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO145_000164"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5465430.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48377..50562
                     /gene="fhuE"
                     /locus_tag="OO145_000165"
                     /pseudo
     CDS             48377..50562
                     /gene="fhuE"
                     /locus_tag="OO145_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OO145_000166"
     CDS             complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OO145_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5465431.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52350..53147)
                     /locus_tag="OO145_000167"
     CDS             complement(52350..53147)
                     /locus_tag="OO145_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5465432.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OO145_000168"
     CDS             complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OO145_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5465433.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54159..54800)
                     /locus_tag="OO145_000169"
     CDS             complement(54159..54800)
                     /locus_tag="OO145_000169"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5465434.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OO145_000170"
     CDS             complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OO145_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5465435.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OO145_000171"
     CDS             complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OO145_000171"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5465436.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OO145_000172"
     CDS             complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OO145_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5465437.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OO145_000173"
     CDS             complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OO145_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5465438.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OO145_000174"
     CDS             complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OO145_000174"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5465439.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OO145_000175"
     CDS             complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OO145_000175"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5465440.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OO145_000176"
     CDS             complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OO145_000176"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5465441.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OO145_000177"
     CDS             complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OO145_000177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5465442.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OO145_000178"
     CDS             complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OO145_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5465443.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OO145_000179"
     CDS             complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OO145_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5465444.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63328..63912
                     /gene="yceF"
                     /locus_tag="OO145_000180"
     CDS             63328..63912
                     /gene="yceF"
                     /locus_tag="OO145_000180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5465445.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OO145_000181"
     CDS             complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OO145_000181"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5465446.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65556..68747
                     /gene="rne"
                     /locus_tag="OO145_000182"
     CDS             65556..68747
                     /gene="rne"
                     /locus_tag="OO145_000182"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5465447.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OO145_000183"
                     /pseudo
     CDS             complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OO145_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OO145_000184"
                     /pseudo
     CDS             complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OO145_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OO145_000185"
     CDS             complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OO145_000185"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5465448.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OO145_000186"
     CDS             complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OO145_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5465449.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OO145_000187"
     CDS             complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OO145_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5465450.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OO145_000188"
     CDS             complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OO145_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5465451.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OO145_000189"
                     /pseudo
     CDS             complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OO145_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76098..77306)
                     /gene="flgE"
                     /locus_tag="OO145_000190"
     CDS             complement(76098..77306)
                     /gene="flgE"
                     /locus_tag="OO145_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5465452.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77331..78026)
                     /gene="flgD"
                     /locus_tag="OO145_000191"
     CDS             complement(77331..78026)
                     /gene="flgD"
                     /locus_tag="OO145_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5465453.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78038..78441)
                     /gene="flgC"
                     /locus_tag="OO145_000192"
                     /pseudo
     CDS             complement(78038..78441)
                     /gene="flgC"
                     /locus_tag="OO145_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78445..78861)
                     /gene="flgB"
                     /locus_tag="OO145_000193"
     CDS             complement(78445..78861)
                     /gene="flgB"
                     /locus_tag="OO145_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5465454.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79016..79675
                     /gene="flgA"
                     /locus_tag="OO145_000194"
     CDS             79016..79675
                     /gene="flgA"
                     /locus_tag="OO145_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5465455.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79751..80044
                     /gene="flgM"
                     /locus_tag="OO145_000195"
     CDS             79751..80044
                     /gene="flgM"
                     /locus_tag="OO145_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5465456.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80049..80465
                     /gene="flgN"
                     /locus_tag="OO145_000196"
     CDS             80049..80465
                     /gene="flgN"
                     /locus_tag="OO145_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5465457.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80505..82040)
                     /gene="murJ"
                     /locus_tag="OO145_000197"
     CDS             complement(80505..82040)
                     /gene="murJ"
                     /locus_tag="OO145_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5465458.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82240..83073)
                     /locus_tag="OO145_000198"
     CDS             complement(82240..83073)
                     /locus_tag="OO145_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5465459.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83075..83722)
                     /locus_tag="OO145_000199"
     CDS             complement(83075..83722)
                     /locus_tag="OO145_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5465460.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83733..84317)
                     /gene="rimJ"
                     /locus_tag="OO145_000200"
     CDS             complement(83733..84317)
                     /gene="rimJ"
                     /locus_tag="OO145_000200"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5465461.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84553..85761
                     /gene="mdtH"
                     /locus_tag="OO145_000201"
     CDS             84553..85761
                     /gene="mdtH"
                     /locus_tag="OO145_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5465462.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85825..86472
                     /gene="grxB"
                     /locus_tag="OO145_000202"
     CDS             85825..86472
                     /gene="grxB"
                     /locus_tag="OO145_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5465463.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86606..87166
                     /locus_tag="OO145_000203"
     CDS             86606..87166
                     /locus_tag="OO145_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5465464.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87272..88318
                     /gene="pyrC"
                     /locus_tag="OO145_000204"
     CDS             87272..88318
                     /gene="pyrC"
                     /locus_tag="OO145_000204"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5465465.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88392..88637
                     /gene="dinI"
                     /locus_tag="OO145_000205"
     CDS             88392..88637
                     /gene="dinI"
                     /locus_tag="OO145_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5465466.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88927..89181
                     /gene="bssS"
                     /locus_tag="OO145_000206"
     CDS             88927..89181
                     /gene="bssS"
                     /locus_tag="OO145_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5465467.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89296..90414
                     /gene="solA"
                     /locus_tag="OO145_000207"
     CDS             89296..90414
                     /gene="solA"
                     /locus_tag="OO145_000207"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5465468.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90462..90575
                     /locus_tag="OO145_000208"
     CDS             90462..90575
                     /locus_tag="OO145_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5465469.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90836..91402
                     /locus_tag="OO145_000209"
     CDS             90836..91402
                     /locus_tag="OO145_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5465470.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91406..91981
                     /locus_tag="OO145_000210"
     CDS             91406..91981
                     /locus_tag="OO145_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5465471.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92023..93075)
                     /locus_tag="OO145_000211"
     CDS             complement(92023..93075)
                     /locus_tag="OO145_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5465472.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93300..94220
                     /gene="lpxL"
                     /locus_tag="OO145_000212"
     CDS             93300..94220
                     /gene="lpxL"
                     /locus_tag="OO145_000212"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5465473.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94392..94589
                     /locus_tag="OO145_000213"
     CDS             94392..94589
                     /locus_tag="OO145_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5465474.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94613..>95523
                     /locus_tag="OO145_000214"
     CDS             94613..>95523
                     /locus_tag="OO145_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5465475.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        22987 a        25313 c        23567 g        23656 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaata gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaagttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgctg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctattcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcgaaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    47461 aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag gttgaatatc
    47521 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    47581 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    47641 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    47701 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    47761 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    47821 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    47881 gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct
    47941 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    48001 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    48061 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    48121 agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg
    48181 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    48241 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc agcaaaatta
    48301 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
    48361 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagtcat
    48421 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
    48481 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
    48541 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
    48601 acatgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
    48661 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
    48721 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
    48781 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactgtt
    48841 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
    48901 tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag gcgatgtctc
    48961 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
    49021 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
    49081 ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
    49141 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
    49201 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag
    49261 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
    49321 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
    49381 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
    49441 gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt ggaacagtgg
    49501 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
    49561 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt cagttcatgg
    49621 gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa
    49681 gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa atcgttatat
    49741 gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc
    49801 aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct
    49861 ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc
    49921 cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac
    49981 tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc
    50041 ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac
    50101 ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc
    50161 aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta tcttgcagag
    50221 gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc
    50281 agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat
    50341 cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac
    50401 gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac
    50461 gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat cgtctacggc
    50521 gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag
    50581 gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac
    50641 tcatccatct cggttttcag gttatcggat ttagtaccga aaatagcctg aacaccagaa
    50701 ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac
    50761 acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct
    50821 ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt tgcttttgca
    50881 tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg
    50941 aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag ccacagtttg
    51001 ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca
    51061 cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg
    51121 tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat accggtcagg
    51181 aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta
    51241 gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca
    51301 ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa
    51361 acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg
    51421 tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact
    51481 accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc
    51541 caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca
    51601 aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta
    51661 aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt atccgccaag
    51721 tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg
    51781 gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg
    51841 gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg
    51901 cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg
    51961 acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag cgatttaccg
    52021 accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg
    52081 tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc
    52141 taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct
    52201 atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca
    52261 atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc
    52321 cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg
    52381 atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca
    52441 cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt
    52501 cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa tcgatccagg
    52561 gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac aatgccggaa
    52621 aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa
    52681 cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg
    52741 gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata
    52801 tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata ataatccagc
    52861 ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca
    52921 tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt
    52981 tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa
    53041 aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat
    53101 tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta
    53161 aagatgagga accggtaaca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag
    53221 atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg
    53281 gcaaacatcc cccagtatag cctgcaggca cgagggagaa agatggtttg ccagttcggc
    53341 gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc gttttagcgc
    53401 atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc
    53461 cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt
    53521 ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc
    53581 gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca
    53641 agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac gactacgtaa
    53701 tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg
    53761 ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt
    53821 tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt cggtgacctc
    53881 acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgtagtaatc
    53941 gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc
    54001 ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat
    54061 gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac
    54121 cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc
    54181 cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca
    54241 atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta
    54301 aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt
    54361 aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa atccccgaga
    54421 acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc gccctgatac
    54481 gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc cagcgcaggt
    54541 ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta
    54601 tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg
    54661 gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg
    54721 agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg
    54781 acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt
    54841 gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata
    54901 gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtagc
    54961 tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag
    55021 gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggttgggtc
    55081 ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg
    55141 ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca gctgattttt
    55201 atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat
    55261 tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat acatcaaggt
    55321 gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac
    55381 ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac gcaattgctt
    55441 gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg cttctttacc
    55501 gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc
    55561 ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa cccgtgggcg
    55621 attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg tccctggctt
    55681 cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt
    55741 ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga taatcaataa
    55801 cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt
    55861 gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat taacgcatta
    55921 caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac ttcgacaagc
    55981 tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc gttaacacct
    56041 gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa attagccgca
    56101 cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc
    56161 tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat acctgcaaga
    56221 tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc gttacgcaaa
    56281 cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg tccgctgttc
    56341 aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag cacaccattt
    56401 tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa gtcacaggaa
    56461 atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa caaactgact
    56521 ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg
    56581 cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc tccttatcag
    56641 agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa
    56701 aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc tttttaaaga
    56761 tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc
    56821 tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca
    56881 ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagattcta
    56941 ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac
    57001 ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta tcgccagccg
    57061 gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac
    57121 gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat
    57181 gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt
    57241 ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaagc
    57301 catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata
    57361 atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc tctgcgccac
    57421 ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt
    57481 gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg ccatacatga
    57541 tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct
    57601 tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa
    57661 tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag
    57721 aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc
    57781 gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt
    57841 tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa
    57901 gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg
    57961 tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat gattctagtg
    58021 ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg
    58081 gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc
    58141 cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga
    58201 atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc
    58261 cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt
    58321 cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca
    58381 actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata
    58441 acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc
    58501 attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc
    58561 cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc
    58621 gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag
    58681 cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg
    58741 tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc
    58801 gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg
    58861 accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga
    58921 aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc
    58981 acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt
    59041 ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt
    59101 ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag tgccaataac
    59161 tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc
    59221 ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcgagcgc
    59281 cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt
    59341 cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta cgccttgcgc
    59401 tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc
    59461 gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca caacagaaac
    59521 tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt
    59581 catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt ttgcgccagc
    59641 agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa taaccacctg
    59701 tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt cttcacacgc
    59761 tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg tgccttccgg
    59821 tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc
    59881 aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg ccatcattgc
    59941 cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg cagtcaacag
    60001 cgctggctgg gtttgccagg ttttattcag ttcttcagct gacccctgct gggtcagcgc
    60061 ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata
    60121 gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa
    60181 tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag agccccaagt
    60241 gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg
    60301 tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag
    60361 cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac tgataatacg
    60421 caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tattcgccgc
    60481 cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc
    60541 cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg gcagcgtcag
    60601 caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg gctcttcaga
    60661 ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc ctcgatctgt
    60721 tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc
    60781 agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac aggctgctgc
    60841 aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac aagctgcgct
    60901 cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc
    60961 aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc
    61021 gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga
    61081 ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc
    61141 agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact
    61201 tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt
    61261 ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa
    61321 cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg
    61381 ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact
    61441 ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata
    61501 acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca
    61561 tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg tcacaaacca
    61621 gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag
    61681 aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc tttacttctt
    61741 cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca
    61801 gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg acgttggccc
    61861 ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc accagcgccg
    61921 gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg
    61981 tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc agggccatac
    62041 gcattgaact cccacggctg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa
    62101 ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta
    62161 atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca
    62221 gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta
    62281 acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc
    62341 ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt
    62401 gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt
    62461 tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg
    62521 tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt
    62581 tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa
    62641 tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg cttcggacac
    62701 ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc
    62761 aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc
    62821 cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac
    62881 ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc
    62941 gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc
    63001 actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc
    63061 ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac
    63121 cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc
    63181 ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc
    63241 agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc
    63301 aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca
    63361 tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc acctgaggtc
    63421 gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa
    63481 aaatcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta
    63541 tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa
    63601 ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg
    63661 gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg tcacctaagc
    63721 gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc gggtagcttt
    63781 aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga ccctaacacg
    63841 ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg
    63901 ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat cggtgcaggc
    63961 ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc ttatatgctt
    64021 gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc
    64081 ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag
    64141 gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc
    64201 gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg
    64261 agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac
    64321 tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct
    64381 ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact gccactgacc
    64441 gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct catgcagaga
    64501 acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc ggtcgagacg
    64561 atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc
    64621 gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat
    64681 atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga
    64741 aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct catcacccgc
    64801 ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac ctttacgcaa
    64861 aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat
    64921 acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt
    64981 catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttata
    65041 tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa
    65101 tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt
    65161 gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac
    65221 aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa
    65281 gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg
    65341 aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca
    65401 tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc
    65461 gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc
    65521 ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa
    65581 ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata
    65641 tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca
    65701 ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc
    65761 cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca
    65821 acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc
    65881 gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc
    65941 tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc gatgaccgta
    66001 ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc
    66061 gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgcctga
    66121 aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc
    66181 aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat atcggcgaaa
    66241 tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct gcattaggtc
    66301 gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact
    66361 accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccatccggtg
    66421 gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac tccgcacgcg
    66481 cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa gccgccgatg
    66541 agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg
    66601 acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc
    66661 aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc
    66721 gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg
    66781 gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ttgatcgaag
    66841 aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt
    66901 cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt caggacggtg
    66961 ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgttgcgcg
    67021 tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg catgaagaag
    67081 cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcac
    67141 tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa cctgccgcga
    67201 ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct gcacaacctg
    67261 ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaagcca
    67321 aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc
    67381 gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac
    67441 gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc
    67501 aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca
    67561 ccgacgagca gcaagtgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc
    67621 aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac
    67681 aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag
    67741 tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg
    67801 ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc
    67861 cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc
    67921 gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg
    67981 gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga
    68041 ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg
    68101 tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc
    68161 cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcgccagttg
    68221 ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg caaccggaag
    68281 tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag ccgcaggtga
    68341 ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa ccgatggttg
    68401 aaccgcagga agagacggca gatgcagata ttgaagaagt tgccgaaact gctgaggttg
    68461 tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag
    68521 cagaagttga aacggtggcc gcggttgaac cagagatcac cgttgagcat aaccacgcta
    68581 ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg
    68641 actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa
    68701 cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa
    68761 gtaatcaagc cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa
    68821 aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc
    68881 cccggcgatc ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg
    68941 agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga
    69001 tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc
    69061 aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag
    69121 cgactccagt ttgttcagct gcgtgcctaa ttccgcgcga acggtaagga cattattcag
    69181 cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc
    69241 ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag
    69301 attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa
    69361 aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat
    69421 actttctgtt ccgccaacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt
    69481 atatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc
    69541 cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt
    69601 agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt
    69661 ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct
    69721 gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga
    69781 ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga
    69841 tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta
    69901 gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc
    69961 caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga
    70021 aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt
    70081 tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa
    70141 ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt
    70201 gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt
    70261 gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt
    70321 aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc
    70381 atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt
    70441 atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc
    70501 gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa
    70561 gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc
    70621 ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga
    70681 acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat
    70741 attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg
    70801 aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg
    70861 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
    70921 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
    70981 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
    71041 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
    71101 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgccgcc
    71161 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
    71221 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
    71281 gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgtatca
    71341 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
    71401 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
    71461 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
    71521 ttattgatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
    71581 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt
    71641 tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat
    71701 agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt tccgcactcg
    71761 cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca
    71821 atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt
    71881 tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca gaggctttga
    71941 caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
    72001 cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
    72061 ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc ggcagcgaat
    72121 catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg ttttgataac
    72181 gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
    72241 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
    72301 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
    72361 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
    72421 tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg ctcgctttcg
    72481 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
    72541 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
    72601 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt
    72661 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
    72721 cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct ggctgacatt
    72781 ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
    72841 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
    72901 cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct gggaactgtt
    72961 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
    73021 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
    73081 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
    73141 accattggtg atccgtccac cgttcagttg gttaacctga acactgctac cgcctgcgga
    73201 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
    73261 gcccttaagc ggagtcatca acaacgtacc tccacgcaag cttttggcat tacccatgga
    73321 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
    73381 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
    73441 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
    73501 agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc ttaccccctg
    73561 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
    73621 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
    73681 aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca
    73741 atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga
    73801 ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca
    73861 cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg
    73921 ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct
    73981 tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag
    74041 ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg
    74101 ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac
    74161 agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg
    74221 ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt
    74281 atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca
    74341 gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt
    74401 tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact
    74461 gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac
    74521 gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct
    74581 ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag
    74641 tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg
    74701 gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc
    74761 cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg
    74821 atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca acattacctg
    74881 gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg acagattgcc
    74941 ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga
    75001 gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag
    75061 atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt
    75121 ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga
    75181 actgatcata taatgtcctg cagtggattg ctgacaagca gagcagaagc acgcgacagt
    75241 gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt
    75301 ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct
    75361 gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca
    75421 atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc
    75481 acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt
    75541 aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt caccagtttc
    75601 agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg
    75661 ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc cgcctcgcct
    75721 atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta
    75781 cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa
    75841 cgtccagcgg gcgagaggtg taatccatct tgccgggcgt catgtctgca cccggtgttg
    75901 acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg
    75961 cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg
    76021 cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg
    76081 agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt
    76141 ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt gaccagttct
    76201 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagagtgcc aaagtttccc
    76261 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    76321 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    76381 tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat ttgataactc
    76441 accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt agcgccggta
    76501 ttttgctgca tggagttgag gaagctcaga ctaaacgtgg cgggttctgc accgttaatt
    76561 gcgccggttg cgatattatt cgccatcgca ccatccacta atgtgccatt agcattaaat
    76621 tccagtgttg tcgctgtctt cgcaatgctg tttggatcac tgctatcctg ggtgtagacc
    76681 tgccagttat tatccccggt cttcacaaaa tagacgctca tgtcatgagc attaccctga
    76741 ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct ggcgctgaat
    76801 ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga cgccgtggtg
    76861 gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt cgccccttgc
    76921 tgaatagtcg gcggcgtacc ggttaccggg taacccgtca gctgtaaacc ttgcgtattc
    76981 aacaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa caccgaaccg
    77041 ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag acctcgcccg
    77101 gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt tacccccagt
    77161 cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag
    77221 gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc agcgtttaat
    77281 ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc
    77341 tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt attaccgctg
    77401 ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac cagttgtgta
    77461 ccaccgttac tggcgctaat cgctacattg taagaaccgt tcggcgcagt gctgccatca
    77521 gtcaacgaac cgtcccaggt gaaactgtga actccggcgg tcagttcacc aatatcaatg
    77581 gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc tgccgcctgt
    77641 tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact gccggttccc
    77701 gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga tcaggttact ggcctgtaac
    77761 gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag tttttcaatc
    77821 ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg
    77881 gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct
    77941 gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt atttgtcgga
    78001 tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc gtaagggttt
    78061 tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg
    78121 ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt atgtagccct
    78181 ttgcatctgc cagcggatta cctggttcat aaaccagttt gtccggggcc tgactttctt
    78241 aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac
    78301 cacctgtttt gcccgatatg gctgtccatc aggaccggtc acgctatcag catttgccag
    78361 attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc
    78421 aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt
    78481 gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat cggcaaactg
    78541 ggtgcgttcg cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg
    78601 gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc gggatgtgct gcgttgaggt
    78661 catcgtcagt gcaaccacac tggttgcatc ccgtccacgt tgcatgactt ttttaagttc
    78721 actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg cgatgtttgc
    78781 tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc
    78841 ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa
    78901 atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt
    78961 tgatggtcat tgcggacagg tacaattcac gttgtagaaa tggctggggg cgaaaatgct
    79021 gacaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa gtacggcgag
    79081 caatctcaca tcgcaattgc acaacttttt tagcgcccaa ctcgcggggg taagtgatga
    79141 ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccatgcgagc agccattgct
    79201 ttcgatgagc aataattccc gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa
    79261 cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg ttgccgcgat
    79321 gcctattgtg cggggaggaa agctggaagc tggcaatgtc aaactgaaac gtggacgcct
    79381 ggataccctg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg ccgttagcct
    79441 gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg catggcgggt
    79501 aaaagcggga caacgcgtca atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga
    79561 aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca tggtatcgga
    79621 acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc tgtaagctgt
    79681 taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg ccctcaatga
    79741 ggaataaacc atgagtattg atcgcacttc gcctctgaag cctgtaagca ccgttcaacc
    79801 gcgcgaaacc accgacgcgc cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac
    79861 cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga
    79921 tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat
    79981 ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa
    80041 ctgatcgtat gacacgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc
    80101 tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc
    80161 agttgcaatg gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac
    80221 agttacgcaa gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg
    80281 aaattactgt gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg
    80341 aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga
    80401 cgctatatgg ggcgaacggt cagacctcaa caccccatcg cggcagtaaa aagatttcga
    80461 tctgaatatt atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg
    80521 gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc
    80581 gccagcacga ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc
    80641 ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga
    80701 cgcaacagaa acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc
    80761 cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca
    80821 tgtttcaacg gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg
    80881 gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc
    80941 actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc
    81001 gcatcaaacg cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga
    81061 atgcccaacg caaccgcact cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc
    81121 atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc
    81181 aaaatggtgc caagcgccac gcccagcaca ccggagggaa actccattaa gcggtcggcg
    81241 taatacatcc aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt
    81301 aaggagatct ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc
    81361 attgctccag catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg
    81421 tgcggtagct gatacaccag ctgcagaatg ccgccgaccg taacagccca cgccagcgcc
    81481 agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta
    81541 agcagtgttg gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc
    81601 accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg
    81661 gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac
    81721 ggtgcggcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa
    81781 acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga
    81841 atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga
    81901 agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca
    81961 attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc
    82021 agcgatttta ataaattcat cggtgttcta atccggaccc acgggcaaaa cgcctgcaag
    82081 gcaggcgctg aaagaggcga atagtctacc tagattatgg tgaattgcta ccgccagatg
    82141 ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag
    82201 cactgcctat tcgccggcgg tttgcggaac tgtctgattt tacacgcatt caatgaagtg
    82261 ccgcgcacag ccgacaaatc cacgttgctc aagagtgctc tgccaaccag gaatcggttt
    82321 attcactaca ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag
    82381 cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca
    82441 agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc
    82501 gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc
    82561 ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac
    82621 gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa
    82681 ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg
    82741 cgccgccagt tccaccagcc tttcagcatc gcgcagattt tctgccagcg gtttatcgac
    82801 acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct
    82861 ggagtgcaca aaaaccgcat cacaactggc ggcaaggctg gataacgaat cggcataagg
    82921 aatgcgccag ctgtcacaaa ttggcagggc tttggcgcgc gtaggcgacc aggctccttg
    82981 taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc
    83041 taatcccact acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg
    83101 aatcaagacg ttgtttcagt tctgccactt cgatttccag ggcttcgacg cgggtctgta
    83161 aatcaccgtc aaccatgttc gacatagccg ttaccgccgg ctgatcttca acctcaccac
    83221 tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa
    83281 aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc gccatatcgc
    83341 tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca
    83401 ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg
    83461 aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta
    83521 gataatgacg tttgaccaga ttatccagct gttcctgcac ttcggattca ctcagattca
    83581 tgaccggttc acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg
    83641 ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaattacg cgagcttcca
    83701 gggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta
    83761 gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca
    83821 tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc
    83881 atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg
    83941 gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga
    84001 tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct
    84061 ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta
    84121 atcatcccta gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc
    84181 tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca
    84241 cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta
    84301 ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag
    84361 tattactata cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta
    84421 ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atactgtccc
    84481 cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc
    84541 ccgggagggg aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc
    84601 atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc
    84661 ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag
    84721 tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa
    84781 ccgatgattg ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc
    84841 cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt
    84901 gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt
    84961 ttctcgctgt tgatgatgca ggacagtgcc agtgcggtca ttggcgcatt gttggggagc
    85021 tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta
    85081 tgtgcggcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc
    85141 gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg
    85201 ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac
    85261 gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg
    85321 ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg
    85381 ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc
    85441 ctacaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct
    85501 gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg
    85561 tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg tggctggctg
    85621 tttgacctgg gcaaatcggc gcaccagcca gagcttccgt ggatgatgct gggaattatt
    85681 ggcatcttca ctttccttgc gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt
    85741 ttgctggaac gcgacgcctg atttgatact cccccctgac tcctccatac tgaaagtgaa
    85801 ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc
    85861 ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc
    85921 aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa
    85981 aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc
    86041 gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag
    86101 gtcaatggct acgtcaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag
    86161 ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat
    86221 tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttgcgt
    86281 gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat
    86341 attcagctat ttccgctact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc
    86401 cgcgttgcta attaccgcga taatatggcg aaacagacac aaatcaattt gttatcatca
    86461 atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca
    86521 gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga
    86581 cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt
    86641 cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat
    86701 taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc
    86761 tgacgcccat attgttctga caaacttgac cagccaaatt ggtcgcgaag agccgaataa
    86821 ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc
    86881 gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt
    86941 cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat
    87001 gcaaacgttg cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc
    87061 ttacgtcctg cgcgaagatg gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg
    87121 cattgaagtg aagccaggcg aaattgtcat tccgtttacg gattaatgac gagggcgcat
    87181 tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa
    87241 aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat
    87301 ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt
    87361 gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt
    87421 gaccacagtt gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg
    87481 gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct
    87541 ggagcgcgga tttaatgaag ccgtgttcac cgctgcaaaa ctttacccgg caaacgcaac
    87601 cactaactcc agccacggcg tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat
    87661 ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat
    87721 ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac
    87781 tgcgctgaaa gtcgtttttg agcacatcac caccaaagat gctgccgact atgtccgtga
    87841 cggaaatgaa cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca
    87901 tatgctggtt ggtggcgtgc gtccgcacct gtattgttta cccatcctca aacgcaatat
    87961 tcaccaacag gcattgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac
    88021 ggattctgcg ccacatgcac gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt
    88081 caacgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca
    88141 gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga
    88201 cacattcatc gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga
    88261 tgacaccctg gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa
    88321 atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac
    88381 agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg
    88441 ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg
    88501 aaggccacgt atcggtacgt tatgccgcag cgaataattt atcggttatt ggcgcaacaa
    88561 aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact
    88621 ggtttgtcag cgaataatat gcagtgattt ttgtttttgc cggatcgccc cggctttttt
    88681 attttactta cgaattttga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata
    88741 tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa
    88801 tttatctgtt ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt
    88861 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
    88921 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
    88981 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
    89041 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
    89101 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
    89161 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
    89221 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
    89281 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
    89341 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
    89401 ccaccgcatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
    89461 gaaggcgaaa agtatgtccc gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc
    89521 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct
    89581 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa
    89641 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
    89701 atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
    89761 atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt
    89821 cgtcatgacg atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa
    89881 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
    89941 gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
    90001 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
    90061 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
    90121 gttccgtttg cggaagtggc cagtgatggg tcagaagcct tcccgttctt gcgcaatgta
    90181 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
    90241 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
    90301 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
    90361 gaccaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
    90421 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
    90481 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg
    90541 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
    90601 aatatttaat ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac
    90661 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
    90721 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
    90781 ttcattctcc gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc
    90841 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
    90901 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
    90961 tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
    91021 gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta
    91081 ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc gcattgtgcc tattgctgtt
    91141 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
    91201 tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg
    91261 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat
    91321 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
    91381 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc
    91441 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
    91501 gcacgccttt gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt
    91561 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa
    91621 tgtgacaatt aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg
    91681 cagtgcggat tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt
    91741 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa
    91801 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg
    91861 tgcaggcttt gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt
    91921 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
    91981 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
    92041 aatacccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
    92101 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
    92161 ctcactacaa caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg
    92221 gcagccatca tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg
    92281 cgcgataatc tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
    92341 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc
    92401 ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca
    92461 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
    92521 gactgctttt ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa
    92581 gtgccccact tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc
    92641 aagcatggcg ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg
    92701 aggatcgtca ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa
    92761 ggatttcccg tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa
    92821 ggcatagagc tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt
    92881 aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata
    92941 taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt
    93001 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
    93061 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
    93121 atatagcaat tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa
    93181 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc
    93241 gggcgaaaaa atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata
    93301 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
    93361 tgggtattgg cgtactttgg ttggtcgtgc aattgcccta cccgattatc taccgcctcg
    93421 gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
    93481 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
    93541 agaatttcga atccgttggc atgggcctga tggaaaccgg tatggcgtgg ttctggccgg
    93601 accgccgaat cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
    93661 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag ctgggcgcgc
    93721 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
    93781 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
    93841 atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc
    93901 atgattacgg cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga
    93961 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
    94021 cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccacca gagtgttctc
    94081 cgccactgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
    94141 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag
    94201 gcgttccttc acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta
    94261 ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
    94321 gggcgcataa ttagtgtgtt tatcttttct tcttatgttc accgcgcctg gcgcaccaac
    94381 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
    94441 tcgtcgcctg gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct
    94501 tacccctcta cgttgagcag cttggtgtta ccggccactc cgccctgaat atgtggtccg
    94561 gtattgtctt cagtataatg ggttcctaaa cacatcattt taggtattga atatgagacg
    94621 ggcaatcagc tacatcagat tttcaagtga acgacaactg aaaggggact ctgtacgccg
    94681 acaatctaag ctggttactg actggcttga taagaaccct gagttttact tggattcatc
    94741 cttgagcttt aaagatttgg ggaaaagtgc ttttagtggt aagcacctga aaggtggtct
    94801 aggtgacttt ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctactaat
    94861 cgagagttta gaccgtctgt cacgtcagga tattgatatt gcttctgagc tattacgccg
    94921 tatcttaagg gctggtgtag atgtagtcac actgagcgat ggagaacatt acacacgaga
    94981 aagtttaaaa gacccgttag ccctgattaa aagcattctg ataatgcagc gggcgcatga
    95041 ggaaagtttg agaaagtctg agcgtgtcca agctgcatgg aaccgtaaaa aagaactcat
    95101 ctccgaaggt atcaaggtat ctaggcgttg tcctgcatgg ttaaggctga atgatgaccg
    95161 tcgaacattc acaatcattc ctgataaagt ggaagttgtt aaacgagcat ttgacctgag
    95221 attacagggt ttaagctttt gggctatcac cagaacttta aacgatgaag gtcacttgtc
    95281 attgaatcaa tacacaccca aacaaaaggg gtggtctgat actgctgtaa agaaactgct
    95341 cagaaatcgt gctgtgattg gatgtttcac acctgctgga cgtgaagaag ttcagggtta
    95401 ctaccccgca ataatcagtg agtcactatt ctatcgagta cagcaactga acacagggca
    95461 atatggtagg gcttctgtat caagtaatcc gctatcagtc aacttgttca ggggtatcat
    95521 caa
//
LOCUS       DAJTMX010000003        75634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTMX010000003 DAJTMX010000000
VERSION     DAJTMX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.003"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(435..641)
                     /locus_tag="OO145_000215"
     CDS             complement(435..641)
                     /locus_tag="OO145_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5465476.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OO145_000216"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OO145_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5465477.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OO145_000217"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OO145_000217"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5465478.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OO145_000218"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OO145_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OO145_000219"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OO145_000219"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5465479.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OO145_000220"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OO145_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="OO145_000221"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="OO145_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="OO145_000222"
     CDS             8997..9101
                     /locus_tag="OO145_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5465480.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="OO145_000223"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="OO145_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="OO145_000224"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="OO145_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5465481.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO145_000225"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO145_000225"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5465482.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO145_000226"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO145_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5465483.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="OO145_000227"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="OO145_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5465484.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="OO145_000228"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="OO145_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5465485.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="OO145_000229"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="OO145_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5465486.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="OO145_000230"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="OO145_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5465487.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="OO145_000231"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="OO145_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5465488.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="OO145_000232"
     CDS             complement(18063..18389)
                     /locus_tag="OO145_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465489.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO145_000233"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO145_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5465490.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO145_000234"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO145_000234"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5465491.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO145_000235"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO145_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5465492.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="OO145_000236"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="OO145_000236"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5465493.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="OO145_000237"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="OO145_000237"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5465494.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="OO145_000238"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="OO145_000238"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5465495.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="OO145_000239"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="OO145_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="OO145_000240"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="OO145_000240"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5465496.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="OO145_000241"
     tRNA            31034..31110
                     /locus_tag="OO145_000241"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="OO145_000242"
     tRNA            31119..31203
                     /locus_tag="OO145_000242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="OO145_000243"
     tRNA            31227..31301
                     /locus_tag="OO145_000243"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="OO145_000244"
     tRNA            31336..31410
                     /locus_tag="OO145_000244"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="OO145_000245"
     tRNA            31426..31502
                     /locus_tag="OO145_000245"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="OO145_000246"
     tRNA            31551..31625
                     /locus_tag="OO145_000246"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="OO145_000247"
     tRNA            31663..31737
                     /locus_tag="OO145_000247"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO145_000248"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO145_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5465497.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="OO145_000249"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="OO145_000249"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5465498.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34789..35829
                     /locus_tag="OO145_000250"
     CDS             34789..35829
                     /locus_tag="OO145_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5465499.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="OO145_000251"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="OO145_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5465500.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="OO145_000252"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="OO145_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5465501.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="OO145_000253"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="OO145_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5465502.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPLLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="OO145_000254"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="OO145_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5465503.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="OO145_000255"
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="OO145_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5465504.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="OO145_000256"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="OO145_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5465505.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="OO145_000257"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="OO145_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5465506.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="OO145_000258"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="OO145_000258"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5465507.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /gene="hscC"
                     /locus_tag="OO145_000259"
                     /pseudo
     CDS             43575..45245
                     /gene="hscC"
                     /locus_tag="OO145_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO145_000260"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO145_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5465508.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="OO145_000261"
     CDS             complement(46753..47460)
                     /locus_tag="OO145_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5465509.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="OO145_000262"
     CDS             47562..48116
                     /locus_tag="OO145_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5465510.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="OO145_000263"
     CDS             complement(48126..49553)
                     /locus_tag="OO145_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5465511.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="OO145_000264"
     CDS             complement(49550..50257)
                     /locus_tag="OO145_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5465512.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /locus_tag="OO145_000265"
     CDS             50421..51398
                     /locus_tag="OO145_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5465513.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="OO145_000266"
     CDS             complement(51468..51950)
                     /locus_tag="OO145_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5465514.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="OO145_000267"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="OO145_000267"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5465515.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="OO145_000268"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="OO145_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5465516.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="OO145_000269"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="OO145_000269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5465517.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="OO145_000270"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="OO145_000270"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5465518.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="OO145_000271"
     CDS             57061..57672
                     /locus_tag="OO145_000271"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5465519.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="OO145_000272"
                     /gene_synonym="rsfA"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="OO145_000272"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5465520.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="OO145_000273"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="OO145_000273"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5465521.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="OO145_000274"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="OO145_000274"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5465522.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="OO145_000275"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="OO145_000275"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5465523.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="OO145_000276"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="OO145_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5465524.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="OO145_000277"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="OO145_000277"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5465525.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /locus_tag="OO145_000278"
     CDS             64327..64590
                     /locus_tag="OO145_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5465526.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="OO145_000279"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="OO145_000279"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5465527.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="OO145_000280"
     CDS             65591..66544
                     /locus_tag="OO145_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5465528.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="OO145_000281"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="OO145_000281"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5465529.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO145_000282"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO145_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5465530.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="OO145_000283"
     CDS             complement(68151..68939)
                     /locus_tag="OO145_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5465531.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="OO145_000284"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="OO145_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5465532.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO145_000285"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO145_000285"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5465533.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO145_000286"
     CDS             complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO145_000286"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5465534.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="OO145_000287"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="OO145_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5465535.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="OO145_000288"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="OO145_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="OO145_000289"
     CDS             complement(73107..73616)
                     /locus_tag="OO145_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5465536.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73779..74024
                     /locus_tag="OO145_000290"
     CDS             73779..74024
                     /locus_tag="OO145_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5465537.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..75630
                     /locus_tag="OO145_000291"
                     /pseudo
     CDS             73999..75630
                     /locus_tag="OO145_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18615 a        18993 c        19210 g        18816 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ctaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg tacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgctgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat agttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc accaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat gtataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75481 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75541 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75601 gatccacagc agttacacag cacggtttat tgag
//
LOCUS       DAJTMX010000004        70712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTMX010000004 DAJTMX010000000
VERSION     DAJTMX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.004"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..796
                     /locus_tag="OO145_000292"
     CDS             <1..796
                     /locus_tag="OO145_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5465538.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1898
                     /locus_tag="OO145_000293"
     CDS             807..1898
                     /locus_tag="OO145_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465539.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1941..2801)
                     /gene="rsmI"
                     /locus_tag="OO145_000294"
     CDS             complement(1941..2801)
                     /gene="rsmI"
                     /locus_tag="OO145_000294"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5465540.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2866..4902
                     /gene="lpoA"
                     /locus_tag="OO145_000295"
     CDS             2866..4902
                     /gene="lpoA"
                     /locus_tag="OO145_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5465541.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4860..5255
                     /locus_tag="OO145_000296"
     CDS             4860..5255
                     /locus_tag="OO145_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5465542.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5275..5865
                     /gene="diaA"
                     /locus_tag="OO145_000297"
     CDS             5275..5865
                     /gene="diaA"
                     /locus_tag="OO145_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5465543.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5875..6450
                     /gene="yraP"
                     /locus_tag="OO145_000298"
     CDS             5875..6450
                     /gene="yraP"
                     /locus_tag="OO145_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5465544.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6564..7604)
                     /locus_tag="OO145_000299"
     CDS             complement(6564..7604)
                     /locus_tag="OO145_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5465545.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7677..8312)
                     /locus_tag="OO145_000300"
     CDS             complement(7677..8312)
                     /locus_tag="OO145_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5465546.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8440..8958
                     /gene="yhbO"
                     /locus_tag="OO145_000301"
     CDS             8440..8958
                     /gene="yhbO"
                     /locus_tag="OO145_000301"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5465547.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8938..9381)
                     /locus_tag="OO145_000302"
     CDS             complement(8938..9381)
                     /locus_tag="OO145_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5465548.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9432..9734
                     /gene="yhbQ"
                     /locus_tag="OO145_000303"
     CDS             9432..9734
                     /gene="yhbQ"
                     /locus_tag="OO145_000303"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5465549.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9721..10224)
                     /locus_tag="OO145_000304"
     CDS             complement(9721..10224)
                     /locus_tag="OO145_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5465550.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10218..10742)
                     /locus_tag="OO145_000305"
     CDS             complement(10218..10742)
                     /locus_tag="OO145_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5465551.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10951..11946
                     /locus_tag="OO145_000306"
     CDS             10951..11946
                     /locus_tag="OO145_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5465552.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11955..12833
                     /locus_tag="OO145_000307"
     CDS             11955..12833
                     /locus_tag="OO145_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5465553.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12914..13921
                     /locus_tag="OO145_000308"
     CDS             12914..13921
                     /locus_tag="OO145_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5465554.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14039..15283)
                     /gene="mtr"
                     /locus_tag="OO145_000309"
     CDS             complement(14039..15283)
                     /gene="mtr"
                     /locus_tag="OO145_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5465555.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15437..17326)
                     /gene="deaD"
                     /locus_tag="OO145_000310"
     CDS             complement(15437..17326)
                     /gene="deaD"
                     /locus_tag="OO145_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5465556.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17319..17399)
                     /gene="yrbN"
                     /locus_tag="OO145_000311"
     CDS             complement(17319..17399)
                     /gene="yrbN"
                     /locus_tag="OO145_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5465557.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17506..18390)
                     /gene="nlpI"
                     /locus_tag="OO145_000312"
     CDS             complement(17506..18390)
                     /gene="nlpI"
                     /locus_tag="OO145_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5465558.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18499..20634)
                     /gene="pnp"
                     /locus_tag="OO145_000313"
     CDS             complement(18499..20634)
                     /gene="pnp"
                     /locus_tag="OO145_000313"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5465559.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20881..21150)
                     /gene="rpsO"
                     /locus_tag="OO145_000314"
     CDS             complement(20881..21150)
                     /gene="rpsO"
                     /locus_tag="OO145_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5465560.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21299..22243)
                     /gene="truB"
                     /locus_tag="OO145_000315"
     CDS             complement(21299..22243)
                     /gene="truB"
                     /locus_tag="OO145_000315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5465561.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22243..22644)
                     /gene="rbfA"
                     /locus_tag="OO145_000316"
     CDS             complement(22243..22644)
                     /gene="rbfA"
                     /locus_tag="OO145_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5465562.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22808..25456)
                     /gene="infB"
                     /locus_tag="OO145_000317"
     CDS             complement(22808..25456)
                     /gene="infB"
                     /locus_tag="OO145_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5465563.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25481..26968)
                     /gene="nusA"
                     /locus_tag="OO145_000318"
     CDS             complement(25481..26968)
                     /gene="nusA"
                     /locus_tag="OO145_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5465564.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26996..27448)
                     /gene="rimP"
                     /locus_tag="OO145_000319"
     CDS             complement(26996..27448)
                     /gene="rimP"
                     /locus_tag="OO145_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5465565.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27655..27731)
                     /locus_tag="OO145_000320"
     tRNA            complement(27655..27731)
                     /locus_tag="OO145_000320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27695..27697),aa:Met,seq:cat)
     gene            28079..29422
                     /gene="argG"
                     /locus_tag="OO145_000321"
     CDS             28079..29422
                     /gene="argG"
                     /locus_tag="OO145_000321"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5465566.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29430..31037)
                     /locus_tag="OO145_000322"
                     /pseudo
     CDS             complement(29430..31037)
                     /locus_tag="OO145_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31515..31601)
                     /locus_tag="OO145_000323"
     tRNA            complement(31515..31601)
                     /locus_tag="OO145_000323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31565..31567),aa:Leu,seq:gag)
     gene            complement(31616..31948)
                     /gene="secG"
                     /locus_tag="OO145_000324"
     CDS             complement(31616..31948)
                     /gene="secG"
                     /locus_tag="OO145_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5465567.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32176..33513)
                     /gene="glmM"
                     /locus_tag="OO145_000325"
     CDS             complement(32176..33513)
                     /gene="glmM"
                     /locus_tag="OO145_000325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5465568.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33506..34354)
                     /gene="folP"
                     /locus_tag="OO145_000326"
     CDS             complement(33506..34354)
                     /gene="folP"
                     /locus_tag="OO145_000326"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5465569.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34444..36378)
                     /gene="ftsH"
                     /locus_tag="OO145_000327"
     CDS             complement(34444..36378)
                     /gene="ftsH"
                     /locus_tag="OO145_000327"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5465570.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36478..37107)
                     /gene="rlmE"
                     /locus_tag="OO145_000328"
     CDS             complement(36478..37107)
                     /gene="rlmE"
                     /locus_tag="OO145_000328"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5465571.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37233..37526
                     /gene="yhbY"
                     /locus_tag="OO145_000329"
     CDS             37233..37526
                     /gene="yhbY"
                     /locus_tag="OO145_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5465572.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37682..38158)
                     /gene="greA"
                     /locus_tag="OO145_000330"
     CDS             complement(37682..38158)
                     /gene="greA"
                     /locus_tag="OO145_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5465573.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38406..39839
                     /gene="dacB"
                     /locus_tag="OO145_000331"
     CDS             38406..39839
                     /gene="dacB"
                     /locus_tag="OO145_000331"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5465574.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40025..41197)
                     /gene="cgtA"
                     /locus_tag="OO145_000332"
     CDS             complement(40025..41197)
                     /gene="cgtA"
                     /locus_tag="OO145_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5465575.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41213..42178)
                     /locus_tag="OO145_000333"
     CDS             complement(41213..42178)
                     /locus_tag="OO145_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5465576.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42305..42562)
                     /gene="rpmA"
                     /locus_tag="OO145_000334"
     CDS             complement(42305..42562)
                     /gene="rpmA"
                     /locus_tag="OO145_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5465577.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42583..42894)
                     /gene="rplU"
                     /locus_tag="OO145_000335"
     CDS             complement(42583..42894)
                     /gene="rplU"
                     /locus_tag="OO145_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5465578.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43152..44123
                     /gene="ispB"
                     /locus_tag="OO145_000336"
     CDS             43152..44123
                     /gene="ispB"
                     /locus_tag="OO145_000336"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5465579.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44350..44628
                     /gene="sfsB"
                     /locus_tag="OO145_000337"
     CDS             44350..44628
                     /gene="sfsB"
                     /locus_tag="OO145_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5465580.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44676..45935)
                     /gene="murA"
                     /locus_tag="OO145_000338"
     CDS             complement(44676..45935)
                     /gene="murA"
                     /locus_tag="OO145_000338"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5465581.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45990..46244)
                     /gene="ibaG"
                     /locus_tag="OO145_000339"
     CDS             complement(45990..46244)
                     /gene="ibaG"
                     /locus_tag="OO145_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5465582.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46404..46697)
                     /gene="mlaB"
                     /locus_tag="OO145_000340"
     CDS             complement(46404..46697)
                     /gene="mlaB"
                     /locus_tag="OO145_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5465583.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46697..47332)
                     /gene="mlaC"
                     /locus_tag="OO145_000341"
     CDS             complement(46697..47332)
                     /gene="mlaC"
                     /locus_tag="OO145_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5465584.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47351..47902)
                     /gene="mlaD"
                     /locus_tag="OO145_000342"
     CDS             complement(47351..47902)
                     /gene="mlaD"
                     /locus_tag="OO145_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5465585.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47907..48689)
                     /gene="mlaE"
                     /locus_tag="OO145_000343"
     CDS             complement(47907..48689)
                     /gene="mlaE"
                     /locus_tag="OO145_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5465586.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48697..49506)
                     /gene="mlaF"
                     /locus_tag="OO145_000344"
     CDS             complement(48697..49506)
                     /gene="mlaF"
                     /locus_tag="OO145_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5465587.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49716..50681
                     /locus_tag="OO145_000345"
     CDS             49716..50681
                     /locus_tag="OO145_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5465588.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50695..51681
                     /gene="kdsD"
                     /locus_tag="OO145_000346"
     CDS             50695..51681
                     /gene="kdsD"
                     /locus_tag="OO145_000346"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5465589.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51702..52268
                     /gene="kdsC"
                     /locus_tag="OO145_000347"
     CDS             51702..52268
                     /gene="kdsC"
                     /locus_tag="OO145_000347"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5465590.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52265..52840
                     /gene="lptC"
                     /locus_tag="OO145_000348"
     CDS             52265..52840
                     /gene="lptC"
                     /locus_tag="OO145_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5465591.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52809..53366
                     /gene="lptA"
                     /locus_tag="OO145_000349"
     CDS             52809..53366
                     /gene="lptA"
                     /locus_tag="OO145_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5465592.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53373..54098
                     /gene="lptB"
                     /locus_tag="OO145_000350"
     CDS             53373..54098
                     /gene="lptB"
                     /locus_tag="OO145_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5465593.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54146..55579
                     /gene="rpoN"
                     /locus_tag="OO145_000351"
     CDS             54146..55579
                     /gene="rpoN"
                     /locus_tag="OO145_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5465594.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55602..55889
                     /gene="hpf"
                     /locus_tag="OO145_000352"
     CDS             55602..55889
                     /gene="hpf"
                     /locus_tag="OO145_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5465595.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56007..56498
                     /gene="ptsN"
                     /locus_tag="OO145_000353"
     CDS             56007..56498
                     /gene="ptsN"
                     /locus_tag="OO145_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5465596.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56544..57398
                     /gene="rapZ"
                     /locus_tag="OO145_000354"
     CDS             56544..57398
                     /gene="rapZ"
                     /locus_tag="OO145_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5465597.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57395..57667
                     /gene="npr"
                     /locus_tag="OO145_000355"
     CDS             57395..57667
                     /gene="npr"
                     /locus_tag="OO145_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5465598.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57880..58512
                     /gene="yrbL"
                     /locus_tag="OO145_000356"
                     /pseudo
     CDS             57880..58512
                     /gene="yrbL"
                     /locus_tag="OO145_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
     gene            complement(58509..59236)
                     /gene="mtgA"
                     /locus_tag="OO145_000357"
                     /pseudo
     CDS             complement(58509..59236)
                     /gene="mtgA"
                     /locus_tag="OO145_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59233..59886)
                     /gene="elbB"
                     /locus_tag="OO145_000358"
     CDS             complement(59233..59886)
                     /gene="elbB"
                     /locus_tag="OO145_000358"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5465599.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60116..62452)
                     /gene="arcB"
                     /locus_tag="OO145_000359"
     CDS             complement(60116..62452)
                     /gene="arcB"
                     /locus_tag="OO145_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5465600.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62548..63477)
                     /locus_tag="OO145_000360"
     CDS             complement(62548..63477)
                     /locus_tag="OO145_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5465601.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64152..68612
                     /gene="gltB"
                     /locus_tag="OO145_000361"
     CDS             64152..68612
                     /gene="gltB"
                     /locus_tag="OO145_000361"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5465602.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68625..70043
                     /gene="gltD"
                     /locus_tag="OO145_000362"
     CDS             68625..70043
                     /gene="gltD"
                     /locus_tag="OO145_000362"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5465603.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNTFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70227..70706
                     /locus_tag="OO145_000363"
     CDS             70227..70706
                     /locus_tag="OO145_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5465604.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17316 a        19176 c        17557 g        16663 t
ORIGIN      
        1 ctggttaccg cgtagcctgg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcct caggagggtg tggagccgac agcactagcg
      901 gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca gataacgtga
      961 caggacggga gtttacctct gcaacgctaa gtagcactaa ctggcaatac gcctgttcct
     1021 gctctgcggg taaggcagtt aaacttgtct atatggtcag ccccgtactt accaccactg
     1081 gacatcagac aggatattac aaactcaatg acagcctgga tattaaaacc acattacagg
     1141 caaacgacat tccaggactc acaaccgacc aggttgtctc tgttaacacc cgattcacac
     1201 agataaaaag cagcaccgta tattctgctg caacccaaac gggtgtttgc cagggtgaca
     1261 cgtctcgtta tggacccgtt aatattggtg caaataccac ctttaccctg tatgtcacca
     1321 agccatttct cggctcgatg accattccga aaacggatat tgccgtcatt aaaggcgcgt
     1381 gggtcgatgg aatgggaagc ccgtctacag gtgacttcca tgatttagtc aagttatcga
     1441 ttcagggaaa tctcaccgcc ccacagtcgt gcaaaattaa tcagggcgat gttattaaag
     1501 ttaattttgg attcatcaat ggtcagaagt ttaccacccg caatgccatg ccagacggtt
     1561 ttactccagt agactttgat atcacttatg actgtggtga tacttcaaag attaaaaact
     1621 cgttgcaaat gcgcatcgac ggtacaactg gggtagtaga ccagtacaac ctggtcgcca
     1681 ggcgaagaag ttcagacaat gtgcccgatg tcggtattcg tattgaaaat ctcggcggcg
     1741 gagttgcaaa tattcctttt cagaacggta tccttcccgt tgatccttcc gggcatggca
     1801 ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg tgagctggaa acaggaaaat
     1861 ttcagggcac agccaccatt accgtcatgg tgcggtaaaa gaagacgttg cgttttgatt
     1921 aacgcaacgc ttcggcactg ttacccctgc tgctccagcg catacttata cagcgcattt
     1981 ttcttcacgc catgaatttc tgcggccagc gccgccgctt ttttcagcgg cagttctgcc
     2041 tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatga
     2101 ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt catcttcctt tacccacgcc
     2161 agcagctcgc caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc
     2221 agaaccacgt agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga
     2281 cggtgggtag attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca
     2341 tcacggcggc cttttgattt ggcaggtaaa aagccttcgt aacagaaacg gtcagatggt
     2401 aaacccgcgg cgcttaacgc agtgatagca gcacacggtc cgggtagcgg caccacacga
     2461 atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt
     2521 ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gctttgccag cagcgtttcg
     2581 gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt
     2641 tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa tcagatcaac ggcctgtaat
     2701 acctctaacg tacgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa
     2761 agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg
     2821 atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt accctcaaca
     2881 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
     2941 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct
     3001 gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg
     3061 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg
     3121 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
     3181 gcagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc
     3241 acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc
     3301 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctt
     3361 ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact
     3421 caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg
     3481 gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
     3541 gccgactggc agaaacgtta tccgaacaat ccgggcgcga aaatgctgcc aacgcagttg
     3601 gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccgctg
     3661 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc cgcgaaaaat
     3721 atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagcagaa
     3781 caacctcagc cgcaaactgc ggatagcgtt gccagcccgg cacaagcctc ggttagcgat
     3841 ctgaccggtg atcagcctgc ggcccagccg gtgcctgtaa gcgccccggc gacaagcacc
     3901 gcagcggtaa gcgcacccgc aaatccatcc gcagagctga aaatctacga tacctcatca
     3961 caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggttggt
     4021 ccgttgctga aaaataacgt tgaagagttg ctgaagagca acaccccgct gaacgtactg
     4081 gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca
     4141 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
     4201 ctggtgctga tcccacgcag tgcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag
     4261 tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
     4321 cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat tactcccaga
     4381 gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat
     4441 gaccagttca ccaataatgg cggtcgtgtc gatgcggtct acattgtggc aacgccgggt
     4501 gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg
     4561 ctgtacgcca gttcccgcag cgcacaaggc accgcaggcc cggatttccg actggagatg
     4621 gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctgcc gttaatgcag
     4681 caggcgctca gcgcggtgaa caacgattat tcgctggctc gcatgtatgc gatgggcgtt
     4741 gatgcctggt cgttggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc
     4801 aacggtaata ccggaagtct gacggctaac ccggattgcg tgattaacag gaagttatca
     4861 tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg
     4921 atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa aggactgcgg tttatcgccg
     4981 ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc ctgaccaccg
     5041 tttttgtcga ggtacgctat cgccgctctg cgctttatgg cggcgcggca gccagtgtga
     5101 cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg
     5161 ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg
     5221 agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa
     5281 gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg
     5341 cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac
     5401 aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc
     5461 atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat
     5521 aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag
     5581 gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc
     5641 cgcgatattg ttaaagcagt tgaagccgcc gttacgcgtg atatgaccat tgtggcattg
     5701 accggctatg acggcggcga acttgcaggt ttgttagggc cacaggatgt ggagatccgc
     5761 attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg
     5821 tgcgatctga tcgataacac gcttttccct caccaggatg attaaggaga atacatgaag
     5881 gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct
     5941 gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc
     6001 acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa
     6061 cagattaaga aagaaacccg tattaatgtc acggcctatc agggcaaagt gctgctggtt
     6121 gggcagtcac caaatgctga actttcggct cgcgccaaac agattgctat gggcgtagac
     6181 ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagca
     6241 tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg
     6301 aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg
     6361 actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg cgtaaagcgg
     6421 gttaccacag cgtttacgtt tattaaatag cagccccttg taatgcctga tgcgacgctt
     6481 gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc gtttacgccg
     6541 catccgacat gttttccctc aaatcagaac aacagcgcca gcccgccgac aatcacacca
     6601 gacactgcca ccatcgcccc tgtcagccat aaggctttcg ccgggaacgc tttgcgcagc
     6661 ataatcagtg acggcaaact cactgccggg agcgtcatca acaatgccag cgccggagcg
     6721 gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc cgtgggaatg
     6781 acaaacaagc atcctgctac cgccatcgcc accacccaca tcaggctgtt atcgacagca
     6841 ccatcggcat gggggaataa ccagacgcgt gcggcaccca acaccagtac tgcaaggatg
     6901 taaaccggga tcgtactcca gaaaagtgtc catagcgccc tgccccagcg gctaaaaaaa
     6961 ccgctctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc
     7021 acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc caccagacga
     7081 atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc cgggtttaac
     7141 accggattgc ccatccagaa tgccagcgca ccgcccatcg atacctgttg gcgacgcatt
     7201 cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga aaacagcgtt
     7261 cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg
     7321 atcagcacct gaatcaacga accgaggatc acccccagca ccgccgcttt ccatactgcg
     7381 aggaaataga tcatcgcgta atccaacgcc gcttgccacg gattagcatc cgcctgcgca
     7441 aggatagatt taccgatact gtgggtttcg gctgcagtaa acgctttgcc gtagtaaggt
     7501 tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc gggtttccac
     7561 cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt ccccaggaat
     7621 aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg gaataattat
     7681 tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca cgccttcatg ctcaggccgc
     7741 atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt ccagttacct
     7801 ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg
     7861 cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc aattaatgct
     7921 tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt ggcacccata
     7981 gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc ggtatccact
     8041 accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg
     8101 ccgagacaac aaaacacaat gtcgatgggt tcggtgacct gcgccaacgc gtcactcagt
     8161 tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg cgtcggcgca
     8221 gcaatggcgt taactttcgg ttcattaatc aacatccgca gcaggtgacc gcccaccagc
     8281 cccgttgcgc ctgtaatcag tacctgactc atcttcgttc ctttacagaa ttgtctgcct
     8341 tgcgctcccc ggctcatgca ccagtcttaa tagaccatga ggtaattatc cccgattgtg
     8401 gaaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa aattgccgtt
     8461 ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga gttccgtaaa
     8521 gccggacacg aagtgattac tattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa
     8581 ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc ggagtttgat
     8641 gccctgttgc taccgggtgg tcattcaccg gattatctgc gtggcgacaa ccgttttgtc
     8701 acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg tcacggtccg
     8761 cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcagt taaaccgatc
     8821 attattgatg ttaaaaatgc gggcgtggaa ttttacgatc aggaagtcgt ggttgataaa
     8881 gatcagctgg ttaccagccg gacaccggac gatctgccag cgtttaaccg cgaggcgtta
     8941 cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt ttaccaaagc ccagcgtgtt
     9001 gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca gcattctggc
     9061 aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac cttccagcct
     9121 gcggatctca cctttaaact gcacaccgcg aattaacgct accgttttcg gctgaccgtt
     9181 taccgttccg gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg ttttttcttc
     9241 cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga taaaaggcat tagcgcacca
     9301 gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca gccaacggct
     9361 gatggcgatg agtgtttcca ttgctgttct cttttatact gtgggcctga ccttaacatg
     9421 cgatgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac aataagcttt
     9481 ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg
     9541 cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca gtgggcgacc
     9601 gttcgctggc actacgggcg gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc
     9661 gtctggtagc ggaaggcgca gggtttgcag aactgttgag cagcctgcaa accccggaga
     9721 ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg gttacgccat tcagcgcgtc
     9781 atccgctaaa cgatgtacca ggaaggcgct ttcagtgccc ggccagcggc aacgcagatc
     9841 gtgatgcgcc gccagttcaa agccgaaacg actgtacagc gccggatcgc ccagcgtcac
     9901 cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca gttggcgtgc
     9961 cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga cccattgcag
    10021 gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct gaccttcgtc
    10081 atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt
    10141 cgcttccgca tcactttcga atgagcgacg cagcagggca tcaatacccg gcgcatcaat
    10201 gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt ttcaggcgcg
    10261 gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg taaggctttc
    10321 ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacacccc cagctccgta
    10381 tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg tttacgcgcc
    10441 gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg cgtgttctgg
    10501 ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat atcacgcaca
    10561 tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag cgcctgacgg
    10621 aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt
    10681 accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag tttatccaac
    10741 acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa aaataacata
    10801 gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa ctttaactgc
    10861 cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta acatttttgc
    10921 gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc cggaaatctc
    10981 ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg gctaaaagat
    11041 gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt gcaggaagcg
    11101 gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat
    11161 ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct gggtgccgac
    11221 gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta cccgcatatt
    11281 gaacgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa cttttatcat
    11341 cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa
    11401 cagctggcac gggttactcc tgtcccgctg gaagtgtttg ctttcggcag cctgtgtatt
    11461 atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc caacactgtg
    11521 ggtgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg gctggaatcc
    11581 cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg ttatccgacg
    11641 ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga agaaccaacc
    11701 agtctcaata ccctggaact gctgccggag ttaatggctg cgaatattgc ttcggtgaaa
    11761 attgaaggcc gccagcgtag cccggcgtat gtcagccagg cggcgaaagt ctggcgtcag
    11821 gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac cgcaaagcgc gtggatggag
    11881 acgctcgggt cgatgtccga aggcacgcaa accacccttg gcgcgtatca ccgtaaatgg
    11941 cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg ccaaaagaga
    12001 cgctggaaga tttttatcag caagccgccg ccagcagcgc cgacgtgatt tatcttggtg
    12061 aagcggtatg cagcaagcgc cgggcaacca aagttggcga ctgtctggag atggcaaaat
    12121 cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg caggcatcct
    12181 ctgaactggg cgaactgaaa cgctatgttg agaacggtga atttctgatt gaagccagcg
    12241 atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc
    12301 tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg atgcgctggt
    12361 gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag tgcgatgagc
    12421 tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta ccgctggcct
    12481 actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct
    12541 gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa caagtgtttg
    12601 tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac gagctggcat
    12661 ccatgcaggg attggttgat gtagtccgcc tgtcaccgca gggcactgac actttcgcga
    12721 tgctcgacgc cttccgcgct aatgaaaatg gcgctgtgcc actgccgttg acggcaaaca
    12781 gcgactgtaa cggctactgg cggcggctgg caggactgga gctgcaagcc taagtaaata
    12841 gctcactttg ttaacaactt taactattct ttaatgcagt attaaagatt aatcggtaac
    12901 aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat
    12961 acccgaaggc tcttcagcgc gggaagcgtt ctcccactct ctcgatctcg cccgtctggc
    13021 tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga ccggcattgc
    13081 cagtgctgca acatcggtat tgattggcta tctggcggcg aataccacca cgctgcatct
    13141 ggggtctggc ggcgtgatgt tgcctaacca cttaccgttg gtcattgccg aacagttcgg
    13201 cacactcaat acgctctatc cggggcgaat cgatttgggg ctgggtcgtg ctccgggtag
    13261 tgaccaacgg acaatgatgg cgctacgtcg tcatatgagc ggcgatattg ataatttccc
    13321 ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg
    13381 cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag
    13441 tgcacaactg gcggcgcagc ttggtctgcc atttgcgttt gcctcacact tcgcgccgga
    13501 tatgttgttc caggcgctgc atctttatcg cagtaacttc aaaccgtcgg ctcggctgga
    13561 aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga
    13621 atttctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccgggca
    13681 actgccgccg ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatgacgt
    13741 gcagcaggcg ctgagtatgt cgttggtagg cgataaagcg aaagtgcgtc atggtttgca
    13801 gtcgatcctg cgcgaaaccg acgctgatga gattatggtt aacgggcaga ttttcgacca
    13861 ccaggcgcgc ctgcattcgt ttgagctggc gatggatgtt aaggaagagt tgttgggata
    13921 gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg taggcctgat
    13981 aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt
    14041 actgatacac cggcagtaaa ttaaagctcg ataaaatatg caccagtgcg ttgccgacgc
    14101 caaacaccag aatcagcgca atcatcggct tgccacccca gacgcggaat ttcgggctgc
    14161 caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc cagatggtag
    14221 ccgctaaacc agcataacca atggcataca ggaatccgtt cgggaacagc agaccaccca
    14281 caacaggcgg ggcaaaggtc agcaatgccg ttttcaagcg gcccacagcc gagtcgtcga
    14341 aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg
    14401 ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc
    14461 cgcttaatgc ttgtaccaac acatcaatat taccgccctt ctcggcgatg ccgataaact
    14521 ccggacgagg gatgttgccc attgtcgcca gcaaccagat ggtatacagc gccagtgcca
    14581 tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca
    14641 tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc agggtcatca
    14701 gcaaatacgg cgcataagat gcattgcttt cggcaacgtt gaacaatgtc gcaggctgca
    14761 catgccccag caggctaccg aaggtgagga agaaggtaat gactttcgcc cccagcacaa
    14821 tcgccgtcat gcggctgacg gctttagtac tcaaccacac cacaaacgct accagcagtg
    14881 caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat
    14941 gcagaatcga accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa
    15001 tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa
    15061 aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca
    15121 tacagaacca ggtaaagatc agcgctgcca ttgaccagaa aaaccacgcc ccggacatga
    15181 ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc
    15241 caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct tctctccagt
    15301 gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacatga gtacaaaaga
    15361 cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat attattttac agattgtgtt
    15421 cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac
    15481 gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacgaccac
    15541 cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgctcgcca ccgaaaccac
    15601 gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt
    15661 tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac
    15721 ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttaccg atgtaacggc
    15781 tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg acgaacttca acaccatcat
    15841 cgcggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg
    15901 gacgatcttc acggtcgcca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat
    15961 cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca atcagagtac
    16021 gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac
    16081 cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt
    16141 ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg
    16201 cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca
    16261 gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg
    16321 tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag ttaaccacca
    16381 ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc ggtcgcaatc aggatgtcca
    16441 gacgaccgtc tttaaggcgt tccagtgtct gctcacgcag cgcctggttc atgtcaccgt
    16501 tcagcgcggc gctattgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt
    16561 ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca
    16621 gtgcttcgtt tttacgcata ccccacacag tccagtagct ctggctgatg tcaggacggg
    16681 tagtcacgct ggactgaata cgcacttcct gcggctcttt cataaagcgg cgggtaatgc
    16741 gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct
    16801 gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat
    16861 ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca
    16921 gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt
    16981 cataacgctg gccgccgtac agggcaacca catttacgcc gcgcatgtgt ttagagaaat
    17041 ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct
    17101 gtggtgcttt cagctcagga tcaagattct gcagcagagg taaagagaat gctgcagttt
    17161 ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac
    17221 actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag
    17281 gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc
    17341 tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt
    17401 gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta
    17461 aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga
    17521 ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg
    17581 ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc
    17641 caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc
    17701 agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct
    17761 aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc
    17821 gaagtgctgt ttcaatactt ctttagcctg cttctcatcg agcttctgct cggcgagata
    17881 aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc
    17941 ttgcgctaac ttgtcacgac cgccgtaata taatgcaatc ccgcgattca agtgcgcgta
    18001 gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt
    18061 gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat
    18121 tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa
    18181 cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag
    18241 gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc
    18301 gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc
    18361 aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga
    18421 acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc
    18481 ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc
    18541 aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac
    18601 ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac
    18661 gcgtttgtca gcgatttgag agatgtgtac cagaccttct ttaccgccgc cgatggcaac
    18721 aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat
    18781 ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc
    18841 tgcaatcttc acagtaccgt catcttcgat ttcgatggtg gtgccagttt cttcggtcag
    18901 ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg ggttgatctt
    18961 gatggtatgg atacgtggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg
    19021 ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat
    19081 gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga taccctcgcg
    19141 ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga
    19201 cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc
    19261 tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga
    19321 agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg ggaatttgtc
    19381 catatccggc atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg
    19441 acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg
    19501 gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc
    19561 agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc
    19621 gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg
    19681 accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatcgc
    19741 gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc
    19801 gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg
    19861 gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt
    19921 taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca gttcattgat
    19981 gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca gcatctggtc
    20041 ttcgctcagc agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac
    20101 aaccagatcc agtttgctct ctttcaactc gtcctgagtc gggttcagta cgtactggtc
    20161 attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac cagacagaga
    20221 cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg ggttaacaga
    20281 aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcggccgaat
    20341 cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc cttcacgacg
    20401 gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag
    20461 tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa caaataccgc
    20521 ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc
    20581 cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat
    20641 atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg
    20701 catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc
    20761 cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat
    20821 tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa
    20881 ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt
    20941 caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg
    21001 gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag
    21061 tgctacctga acttcagtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat
    21121 tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag
    21181 gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac
    21241 gcgacgttaa gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt
    21301 acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc
    21361 ccataccgat aaatttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg
    21421 caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat
    21481 tcaccaccgg gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact
    21541 ccgcagccgg aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg
    21601 tcacaatccg ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg
    21661 cgccacaacc gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg
    21721 agcagtgaat ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa
    21781 cggtaatcgg gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt
    21841 ttttaccctg atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga
    21901 aagtatccag cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga
    21961 tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc
    22021 gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca
    22081 acatgccagt tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa
    22141 tccgtttcac tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca
    22201 aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc
    22261 gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat
    22321 gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag
    22381 gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat
    22441 gcccgcttta accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata
    22501 cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc
    22561 tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg
    22621 cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc
    22681 ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag
    22741 cgtcacatca ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct
    22801 aaaaacctta agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc
    22861 cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt
    22921 cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt
    22981 tgtcacgcag aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc
    23041 ctgcgatggc accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa
    23101 tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca
    23161 ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg
    23221 cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg
    23281 tggcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt
    23341 cagtagacag tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct
    23401 tcaggacgat gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga
    23461 gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg
    23521 caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta
    23581 cgccggacag gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac
    23641 ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc
    23701 ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga
    23761 ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa
    23821 cttccgcctg cagcaggata gcgtccagca gttcatcgat accagtaccc gctttcgcag
    23881 acacatgtac gaactggctt tcaccgcccc actcttccgg caggatgccg tactgggaga
    23941 gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gatcttgttc actgcaacga
    24001 ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc gatcgtctgc ggcattacgc
    24061 cgtcgtcggc agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgagcacgca
    24121 ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt
    24181 caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gccactttcg
    24241 ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca
    24301 caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt
    24361 ccagctcgtt ttcacgacgc aggataactt tatggcccat ctcttcagca accagctgtg
    24421 cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt
    24481 tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg gtgatagttt
    24541 cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcagcg
    24601 aagaaccttt acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat
    24661 cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac
    24721 cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt
    24781 gagaagtagt gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt
    24841 tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt
    24901 tacgacgcgc ttcttcttca gctttacgct tgagctctgc agcttcctgc tcacggcggg
    24961 ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt
    25021 ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac
    25081 gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct
    25141 cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc
    25201 ggacttcgat ttgtaccgat ttgctttttc caccggtacc aggaatgtta agggtgctgc
    25261 gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc aggtggtcaa
    25321 tcaaagtctg tttctcttgt gcagacacag agtcgtcagc agacttccgg atacctgcat
    25381 cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa
    25441 tcgttacatc tgtcatgctg ttccttcctg ctacagttta ttacgcttcg tcaccgaacc
    25501 agcaaatatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga
    25561 tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg
    25621 cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg
    25681 gtttgttatc accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag
    25741 cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt
    25801 tcatcggcac ataggccagt tcttccagcg tcgagaaacc ttcttctacc agaacagtcg
    25861 cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg
    25921 cctgatgctt agcctgcagg tcgtcaacgg tcattacgtt gagttcccaa ccgctcagct
    25981 gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg ttaccagctt
    26041 caacggcgat atccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg
    26101 gtgccattgc gttaatcaca aactgcgccg ggttatcatc ccacaggacg atatcgatac
    26161 gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc gccacgcata cctacgcaag
    26221 cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg
    26281 ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttccggc acttcaatac
    26341 ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc gcgccacgcg
    26401 cttccgggcg aacggaataa agcacgccac gaacgcggtc gccagggcgg aagttttcac
    26461 gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga tccagagaga
    26521 tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga
    26581 actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg
    26641 ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc
    26701 ccaagttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct
    26761 gggtgacttc atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat
    26821 cgatctgtac acggacgtcg atctcttgct catatttttt ctttgttgct gtcgccagcg
    26881 cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt
    26941 caactacagc caaaatttct ttgttcatcg cgggcttttc acctcatcca gactattaaa
    27001 agtggggaac caggttcgcc ttctggatat tactcagcgc gaacacttca tctttacctt
    27061 cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc catttacgac
    27121 ggttttgtac cgccatacgg agaaccagag tcacctcttc tccgacaaaa cgggcgtagt
    27181 gttcagccgt gaacagtggg cgatcgagac ccggtgagga gacttccagg ttataagcaa
    27241 cggtgatggg atcttcaaca tccagcacag cacttacctg gtggctcaca tcagcacaat
    27301 catcaacatt gatgccatct tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc
    27361 gaataaattc gatgcctacc agttcaaaac ccagggcctc aactggcgca gtaatcatct
    27421 ctgttaattt ttgctctaat gtggacaagc ccacccccaa gacataaaaa aagggcctaa
    27481 agcccagtta ttctgtagtc agataacaaa aaaccccgat aaatcggggc tttatataac
    27541 tgaaccctat aaccgcaact gcggtctgga gcactttcca gaaggatttt ttcaaatccc
    27601 actacgaagg ccgaagtctt cacagtatat ttgaaaaagg actctaaggg aaagtggttg
    27661 cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc
    27721 tccaccccgc gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact
    27781 gctgggattt ggtgtaccga gacgggacgt aaaatctgca ggcattatag tgatccatgc
    27841 cacattttgt caacgtttat tgctaatcac gtgaatgaat atccagttca ctttcatttg
    27901 ttgaatactt ttgccttccc ctgctctccc ttaagcgcat tattttacaa aaaacacact
    27961 aaactcttcc tgtctccgat aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa
    28021 attcagtgag agcggaaatc caggctcatc atcagttaat taagcagggt gttattttat
    28081 gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg
    28141 tctggacacc agtgccgcac tgctgtggat gcgacaaaag ggagcggttc cttatgcata
    28201 tactgcaaac ctgggccagc cagacgaaga ggattatgat gcgatccctc gtcgtgccat
    28261 ggaatacggc gcggagaacg cacgtctgat cgactgccgc aaacaactgg tggccgaagg
    28321 tattgccgct attcagtgtg gcgcatttca taacaccacc ggcggcctga cctatttcaa
    28381 cacgacgccg ctgggccgcg ccgtgactgg taccatgctg gttgctgcga tgaaagaaga
    28441 tggcgtgaat atctggggtg acggtagcac ctataaagga aacgatatcg aacgtttcta
    28501 tcgttatggt ctgctgacca atgctgaact gcagatttac aaaccgtggc ttgatactga
    28561 ctttattgat gaactgggcg gccgtcatga gatgtctgaa tttatgattg cctgcggttt
    28621 cgactacaaa atgtctgtcg aaaaagccta ctccacagac tccaacatgc ttggtgcaac
    28681 gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca acccgattat
    28741 gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca gaagaagtca cagtacgctt
    28801 tgagcaaggt catccggtgg cgctgaacgg taaaaccttt agcgacgacg tagaaatgat
    28861 gctggaagct aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc agattgagaa
    28921 ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg cactgctgca
    28981 cattgcgtat gaacgtctgt tgaccggtat tcacaacgaa gacaccattg agcagtatca
    29041 cgcgcatggt cgtcagttgg gccgtctgct gtaccagggg cgttggtttg actcccaggc
    29101 gctgatgctg cgtgactctc tgcaacgctg ggttgccagc cagatcactg gtgaagttac
    29161 cctggagctg cgccgtggga acgattattc aatcctgaat accgtctcag agaacctgac
    29221 ctacaagcca gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg
    29281 tattggtcaa ttgaccatgc gtaatctgga tatcactgat acccgagaga aactttttgg
    29341 ttatgccaaa actggcctgc tttcctcctc tgccacttca ggcctgccgc aggtggagaa
    29401 tatggaaaac aaaggccagt aattcgacat caaccctgcc cttcaggggt ggggttacct
    29461 tgcttatcct ctgtaaactc ctttctgagc atcacataat cgaaaacatc atggtttgca
    29521 tccacaaccc gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt
    29581 tccggctttg ttacccccat ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag
    29641 atattgtttt ccgttgtgcg atccacgcca tccccaagaa cggggctata ccagataaac
    29701 atcgggacat gatatgcctg ctggctagcc tccctgcctc catgaaaata gacgttcttc
    29761 ttcgtagggt cacgttccag gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct
    29821 tttaataatt caaaaacctg acctagcaaa ctatctgtgt aatgaatgga gttgtcatag
    29881 caggcatcct gatcgtcctg cggttgaaac acggcgctgg attgcggata ggcgctacaa
    29941 gccggttcat ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc
    30001 tgtaatgctt gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt
    30061 tccatggcgc gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc
    30121 gagcttagcc agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg
    30181 taattatgaa tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc
    30241 gcagtgtaag gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct
    30301 tcaacttgcg gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca
    30361 gactccccca caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa
    30421 tacggcaccg tgtttgcaat tgagagtaat ctttgatgcg ctttcgctgc taaagcaaaa
    30481 tagttgagat tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg
    30541 aacgcttttt tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc
    30601 gaaatgacaa tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat
    30661 tttattatta ctgccaaaaa aagaagggat aaaaaagcaa aagcacatag ataagggata
    30721 tacatcccca gcattttcac aacttcatcc ggatcactct gcagcacact aatcgcaaat
    30781 ccatcattga aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc
    30841 gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt
    30901 ttaacaacca ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgctccggaa
    30961 gaggcgacta agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat
    31021 ttaaaatctt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    31081 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    31141 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcgaccatt gcgttctcag
    31201 taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt
    31261 tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa
    31321 ctggagttga cacaataaag tgccaattat gtcagcagta gggaaaagtt tataactaag
    31381 gcgtaccggc accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca
    31441 gatacctgac aaccattcat cagaccagga ccaaaacgaa aagagacgct tttcagcgtc
    31501 tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta
    31561 ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt
    31621 tcgggatatc gctggtcggc ttagccggag cagctggctg agtttgttcg gttttcgccg
    31681 gtgcactcag attttcccat tcgctacctc tattggtttt gttgctattg atgttaccca
    31741 gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt
    31801 taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca
    31861 tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa
    31921 ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta
    31981 ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat
    32041 actaaccaaa acgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa
    32101 aaaaacagca aaaccgatgt gttcgccgag aaaaaaggcg acaatcgccg cctttttagc
    32161 cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt
    32221 cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt
    32281 acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt
    32341 aactgactca tgctcaagcg gatcgccgct acctgcggtg taacgtacgt taaccagaat
    32401 ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat
    32461 cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtggttt tatccagcag
    32521 gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag
    32581 tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag
    32641 ttcaagcccc atgttgctca tcaatgtacc cacagcacca ccacgcagct ggccctgacg
    32701 aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg
    32761 gtcaaccata atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc
    32821 agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg cattgatgtt
    32881 tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt
    32941 cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag
    33001 gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac
    33061 gatacggctg gctttaccca gttcagccga atcaacgcag ctgatctcct tttccatttc
    33121 cgcttcgatg gcctcttcta ccgcatccgg cagtttagtg ccgtcgatag agaagaattt
    33181 aatcccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg
    33241 gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga
    33301 aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg
    33361 cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc
    33421 ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat
    33481 cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag
    33541 tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt
    33601 gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg
    33661 gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca
    33721 ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt
    33781 taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc
    33841 aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg
    33901 gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg
    33961 tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt
    34021 gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc
    34081 agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt
    34141 caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg
    34201 cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc
    34261 caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa
    34321 ggtccagtga agtaccctgg gcaaagagtt tcattatgtt atccctggta tgaattgata
    34381 agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg
    34441 aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc
    34501 atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg
    34561 ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg
    34621 tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt
    34681 gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg
    34741 gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag
    34801 gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca
    34861 ctgagtcacc atgttacgtg ccaggttggt cgcaacttta atatcgttgg acgcaccggt
    34921 agaaacatgt tccggcccgt agatgatctc ttctgccaga cgaccaccgt acagcgtaga
    34981 aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa
    35041 agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc
    35101 cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg
    35161 cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt
    35221 ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc
    35281 gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacgag caatgattgc
    35341 cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga actttcagga tctgctcacg
    35401 accgcgaaca tctggcaagc cgaccacaac ctggcggtcg aaacggccag gacgcagcag
    35461 ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc
    35521 ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc
    35581 accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat
    35641 gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc
    35701 gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc
    35761 tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc
    35821 tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag
    35881 ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat
    35941 ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc
    36001 gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa
    36061 ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt
    36121 gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt
    36181 ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg
    36241 aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga
    36301 ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag
    36361 tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa
    36421 cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta
    36481 gggtttacgc ccggtcgcta caatatacac ttctcgcgaa cgtgcacgag aagagtccgg
    36541 cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc
    36601 ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat
    36661 ttctagcgcc agttccacca gatacatggc tcgggggata tccaccgccg gtgttccgct
    36721 catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag
    36781 cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat
    36841 aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttaccgccaa tttgggtgac
    36901 cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt
    36961 aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaatcc
    37021 ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga
    37081 gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc
    37141 aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc
    37201 cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc
    37261 acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga
    37321 ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga
    37381 aaatcgccac cgaagatcgc gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa
    37441 ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaag
    37501 aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa
    37561 aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg
    37621 ccgcagagcg gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt
    37681 cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga
    37741 ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa
    37801 tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt
    37861 cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca
    37921 ttttggtgac atcaatcacc tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc
    37981 cttcgcagaa gccctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc
    38041 catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa
    38101 aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt
    38161 gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct
    38221 gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc
    38281 caggtcagct acaattcaca ttttgatggt cattttaccc tgaagttccc gaagggtcat
    38341 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    38401 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    38461 aggccgcgaa tgttgatgag tacgttactc aactccccgc aggtgccaac cttgccctga
    38521 tggtgcaaaa agtcggtgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    38581 tgcccgccag tacccagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg
    38641 attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    38701 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata
    38761 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    38821 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    38881 gctttagcgc tccgcctgct gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    38941 acagtgcccc aaagcctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    39001 cgatgttcag ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgaac
    39061 tggatgtggt gccaagcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt
    39121 ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc ggtgcaattc
    39181 tgaaagatga gttaaaacag gcgggtatca cctggagcgg aacactgctg cgccagactc
    39241 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc
    39301 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatcg
    39361 gccatgcgcg cttcaatgtg cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga
    39421 tcctgcgcca gcaagccggt gtcgatattg gtaacaccat tattgccgat ggttcagggc
    39481 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    39541 aacacgacaa tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt
    39601 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg
    39661 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    39721 tggcatttgt acaatatctt tctggctatg cagtaaaacc cgcggatcag cgtaatcgcc
    39781 gtattccgtt agtgcgtttt gaaagccgtt tgtacaaaga tatttatcag aacaattagt
    39841 caaaaagaaa ccccggcgca tggccggggt ttcagattat tgacaaagtg cgctttgttt
    39901 atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt
    39961 gcaatcatct tgtaggcctg ataagtgtag cgcatcaggc ttatttggca ttggtcatta
    40021 gtgattaacg cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat
    40081 cccagtcttc atcatcctct tcagcaatct cttcaagctg ctgacggtga taatcatccc
    40141 acatgaattc gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa
    40201 tgataaaggt catcacatcc cagcagagat ctttcacgcc cagtccgctc gccgcagaga
    40261 tcagataata tttatcttcc cagcccagcg cttcagcgat cgctttcgct ttctcttcgg
    40321 cttctgcctt atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca
    40381 gatcctggct gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg
    40441 tgccgtcaat cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct
    40501 tcaggaagcg aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat
    40561 cggcaacaac gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag
    40621 tggtaaacgg ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg
    40681 atttacccgc gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca
    40741 gcagctcacg cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg
    40801 acgatttgaa acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct
    40861 gaccgtgttt agtcatatca cccatggttt caccagtacc ctggtcgatt acacgcgtac
    40921 ctaccggcac tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc
    40981 cgttctgacc gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca
    41041 ggttctcgtc ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc
    41101 cgcctttcgg aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg
    41161 caacgaccag aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc
    41221 ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac
    41281 caatggcgga atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt
    41341 ttaacatcgg tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca
    41401 gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca
    41461 tcgcttccgc caaggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg
    41521 cgagctgcca gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcga
    41581 ttgtacataa agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa
    41641 ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagataactc
    41701 cccaggtgta atcggtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca
    41761 ccagaccgct caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca
    41821 ggataaatac gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag
    41881 cggtcggact caggtattgc aaggatgagc tgaacaggat gaagttccca aacagcccgg
    41941 cggtcgccac tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca
    42001 acctcttctt caccgcaaga atggcaccaa ggccaatact cgccatcaag aaacggtaaa
    42061 acacgattgt cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc
    42121 cccagcaaat tgctgtggta agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac
    42181 cgtattccct gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt
    42241 atcgaatgta aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa
    42301 aaacttattc agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt
    42361 tcactttacc gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc
    42421 cagcgtggaa tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc
    42481 caccgaaacg cttaacgccc aagcgtttag cttctgaatc gcgaccgtta cgtgtggagc
    42541 cgccagcctt tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt
    42601 aattttcaca tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg
    42661 acgaaactta acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat
    42721 tacgccgcca tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag
    42781 cacttcagcg aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt
    42841 ctgaccttcg cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa
    42901 ctccgcttcc gcgcacacct tttcaatgat tcagagtgcg ctataaatat tcacaatagg
    42961 gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa
    43021 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa ttaccctata
    43081 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    43141 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    43201 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca actgatcaat cagttaggct
    43261 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    43321 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    43381 acacggcgac tctgctacac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    43441 ctaccgccaa cgccgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    43501 cccgcgcgtt ccagatgatg accagcctcg gttcactcaa agtgctggaa gtcatgtcag
    43561 aagctgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    43621 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    43681 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaggaggag aaaggcctgc
    43741 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    43801 atgccgatgg cgaacagtta ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga
    43861 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    43921 ccgccatcga acaaggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    43981 cttgtggatc tcttgaatgg acgcgtcagc gcgccgagga agaagcagac aaagccatcg
    44041 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    44101 tcgctgttca acgcgatcgt taatcccctc ccttcatccc gcgcagtgcg cgggatgatt
    44161 ccagtaaatt ccataaaaac actaattcag cgctataaca cctgaaaaaa gggcgatgaa
    44221 tctttatata ttctgaatat tcacactctt tacaggaact ttttagcgca atagaccatc
    44281 aggagtatag tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    44341 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg
    44401 cgcaagaaag gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg
    44461 ctggcaaacg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    44521 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    44581 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    44641 tagccccgcg aacggggctg ccagctctca gacgattact cgcctttcac acgctcaata
    44701 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    44761 atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat
    44821 gcacgcagat cggttgccat aacctgtgca ccagaaagtt tttcaacacc atgacaaata
    44881 acggtattgc tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg cacatgcata
    44941 aagcggtttt caaagaccgt ttcggtgatg aacccggtcc cttcagccac caggttcaac
    45001 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    45061 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    45121 tccgctccag catcacgcag tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta
    45181 cggcagataa ttttgccacg agagatcgcc gccgccacca ggaaagtacc ggtttcgata
    45241 cgatccggca gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    45301 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    45361 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc cagggttgca
    45421 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    45481 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    45541 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    45601 ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagcgc ccagatagaa
    45661 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    45721 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag tttcattgat
    45781 gtatcgacgt ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtagt
    45841 gcggcaaaaa gaataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    45901 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    45961 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    46021 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    46081 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatacc gtcaaacaac
    46141 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    46201 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    46261 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    46321 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    46381 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    46441 ttcgcgaggg tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt
    46501 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    46561 cggctaagat cgatgcaggt aatccccttc accgcctcct cacgcatttc ccaaagcggt
    46621 agcagaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    46681 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    46741 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    46801 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcttgc cagtttcccg
    46861 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa
    46921 taatggtaac gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    46981 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    47041 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    47101 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    47161 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    47221 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    47281 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    47341 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    47401 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    47461 caatgagatc ttccagcacc atcgcagact tggtgtcctg aattgtatcg ccatccttca
    47521 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttctc
    47581 ccagcaggcc ggaagtacga atgctcagcg aactggtatc aggaatgtgg ttataacgtt
    47641 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    47701 cccggcccac aacaacacca ccaatgctga ccggagagcg cgctttcagg ccgccaatgt
    47761 tatcgaacgt cgcataaagc gtgtaggtcg attcagtacg tatggacgtc acgttcgccg
    47821 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    47881 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    47941 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    48001 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    48061 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    48121 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagcgat
    48181 ccgccccaga tccccacggc gacgaagata accgtcaaca gtggtaatga aataacccca
    48241 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    48301 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttaacgccga accagcacgc
    48361 ccggcaaaca acagcgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    48421 agcataccca gactggtttc cgcgctataa gtggtcagaa ccagataacc ttgcagcccc
    48481 aacaccattc cgatgaacac gccagaaact acaataatca gcatcgacag gacgccgaca
    48541 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    48601 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    48661 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    48721 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    48781 ctgacgtacg cgcggatcag gattcgtctg caacgcctgg gcactgccat gagcgacaat
    48841 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    48901 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    48961 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    49021 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    49081 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    49141 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    49201 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttacgcac
    49261 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    49321 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    49381 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    49441 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    49501 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    49561 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    49621 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    49681 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    49741 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    49801 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    49861 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    49921 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    49981 cgctggttcg tccttttacc gtccattccg atgttctgcg ccgtgaatta cccttaatgt
    50041 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgaagcgatg
    50101 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    50161 aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc
    50221 ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg
    50281 tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatggctc
    50341 tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata gcgggggttc
    50401 gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg
    50461 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca
    50521 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    50581 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    50641 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    50701 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    50761 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    50821 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    50881 atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat
    50941 cctagtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    51001 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    51061 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    51121 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    51181 agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    51241 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    51301 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    51361 gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt
    51421 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    51481 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    51541 cgtgtgcgcc ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    51601 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    51661 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    51721 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc
    51781 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    51841 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    51901 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    51961 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    52021 gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc
    52081 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc
    52141 cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc
    52201 gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    52261 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat
    52321 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    52381 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    52441 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    52501 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    52561 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc
    52621 acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    52681 gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    52741 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    52801 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    52861 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    52921 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    52981 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    53041 gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg ttctaccaga
    53101 tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa
    53161 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    53221 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    53281 agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga
    53341 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    53401 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    53461 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg
    53521 taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    53581 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt
    53641 tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt
    53701 ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc
    53761 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    53821 gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc
    53881 cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg
    53941 tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    54001 tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg
    54061 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    54121 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    54181 actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga
    54241 acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac
    54301 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc
    54361 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    54421 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta
    54481 ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc
    54541 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgaaaccgg
    54601 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat
    54661 cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gacccggtcg gtgtagcggc
    54721 aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga ccacgccgtg
    54781 gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca atcacgactt
    54841 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    54901 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    54961 cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga
    55021 cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    55081 cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    55141 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    55201 agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca
    55261 ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    55321 tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg
    55381 cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg cggcggaaaa
    55441 cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    55501 ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    55561 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    55621 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa tttgccaaac
    55681 ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca
    55741 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    55801 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    55861 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    55921 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    55981 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    56041 gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc
    56101 tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag gtggtttttg
    56161 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    56221 cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa
    56281 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    56341 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg
    56401 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    56461 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    56521 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    56581 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    56641 ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg
    56701 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    56761 acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa
    56821 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    56881 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    56941 ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc
    57001 tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    57061 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    57121 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    57181 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    57241 tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc
    57301 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    57361 agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    57421 cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    57481 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt
    57541 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    57601 tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga
    57661 agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac aataagaaaa
    57721 tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt
    57781 cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac
    57841 catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa
    57901 agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc
    57961 tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta
    58021 aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac
    58081 ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc cgattttgac
    58141 ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt
    58201 gcacaactgc gccagctact gaaatagctg aagcgttatt tgcaggataa ccgtatcgtg
    58261 acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc
    58321 ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag
    58381 tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg
    58441 gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg
    58501 cgcgaagctt aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc
    58561 tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa
    58621 cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc
    58681 gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat
    58741 tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc
    58801 agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga
    58861 ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    58921 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    58981 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    59041 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga
    59101 ccattaccgc tgagaaggga acaggcgcaa cgctaaacaa cgcgatgccc ccgccccaga
    59161 aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta
    59221 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    59281 cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc
    59341 ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca tctcttccag
    59401 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    59461 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    59521 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagac tggcaaaatt
    59581 gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    59641 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    59701 attgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt
    59761 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    59821 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    59881 cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    59941 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    60001 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc
    60061 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    60121 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    60181 tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca ggagactgaa
    60241 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    60301 tatgtccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    60361 tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    60421 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    60481 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    60541 tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga
    60601 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    60661 cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    60721 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    60781 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcgacaatca
    60841 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    60901 catcaaacgc atcatcgacc tcttctgcca ccgacggtgc gtggatcgtc aacgtaaagg
    60961 ttgaaccttt gccctgttca ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    61021 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    61081 tcacctggta atacatggcg aagattttat ccagctcatc ctgcggaatg ccgataccag
    61141 aatcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    61201 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    61261 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt aggctccagg ttaaagcgca
    61321 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    61381 aatcaaccgg ctgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    61441 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatagttttg agatattttt
    61501 cctgctcggc ggtgagttcg gtatccagca gaatgcggct caggccgacg ataccgttca
    61561 gcggtgtacg caattcgtga ctgatggtgg aaataaacgt cgttttgtcg cggctggcgc
    61621 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    61681 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc
    61741 gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt
    61801 tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca
    61861 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    61921 aaaactcttt atcttcgtta cgataaaaaa ccaagtcggg tgaagcatca aggaaggaac
    61981 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    62041 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcaattt
    62101 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    62161 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    62221 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    62281 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    62341 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    62401 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    62461 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    62521 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtagccacg gacgtcccag
    62581 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    62641 tcgattttcg caccacagcg gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    62701 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtataatc
    62761 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    62821 cacaatatgc agcggatgca gctttatgcc gtccacgctg gtttcaacca ctcgctccag
    62881 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac
    62941 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    63001 gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    63061 ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    63121 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    63181 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    63241 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    63301 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat
    63361 ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat gcaccttttg
    63421 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    63481 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    63541 gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca aaattaccga
    63601 aatttcatgc acaatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    63661 cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa
    63721 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    63781 aggaattgac tgtttctcta acgactctcc ttttagcctt aaagataaaa tctattttaa
    63841 tttcagctat ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    63901 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    63961 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    64021 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    64081 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    64141 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    64201 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    64261 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
    64321 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    64381 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    64441 ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt gtgggctggc
    64501 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca
    64561 ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc acgcgatatg gagcgccgtc
    64621 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    64681 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
    64741 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catttgcctg ttccaccagc
    64801 gcttctccac taacaccgta ccgcgctggc cgctggcgca accgttccgc tatctggcgc
    64861 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
    64921 aattccagac gccgctgatc cctgacctgc acgacgccgc gccattcgtc aacgaaaccg
    64981 gttctgactc cagttccatg gataacatgc tggaactgct gctggcaggc gggatggata
    65041 tcatccgcgc catgcgcctg ttagtgccac ccgcctggca gaacaacccg gatatggacc
    65101 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggtccgg
    65161 cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
    65221 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
    65281 tctgggatta ccagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
    65341 tggtgatcga cactcgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    65401 aaagccgcca cccttataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    65461 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    65521 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
    65581 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
    65641 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
    65701 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
    65761 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
    65821 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
    65881 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctat
    65941 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgtt gctggtgctc tccgaccgga
    66001 atatcgctaa agatcgcctg ccggttccgg ctccgatggc ggttggcgcg atccagaccc
    66061 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    66121 gcgatccgca ccacttcgcc gtgttgctgg gtttcggcgc gacggctatt tatccgtacc
    66181 ttgcctatga aacgctgggc cgcctggtag acacccacgc gattgccaaa gattatcgta
    66241 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    66301 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctac
    66361 acgatgatgt agtgggcctg tgcttccagg gcgcggtcag ccgcattggt ggggcaagct
    66421 ttgaagactt ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc
    66481 cgattagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcctacaacc
    66541 cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc gactatcagg
    66601 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    66661 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    66721 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    66781 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
    66841 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
    66901 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    66961 agccgggcga aggtggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
    67021 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
    67081 tcgaagactt agcgcagctt attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    67141 ccgttaagct ggtgtccgaa ccaggcgttg gcaccatcgc gactggcgtg gcgaaagctt
    67201 atgcggactt gatcaccatc gcgggctatg acggcggcac cggcgcaagc ccgctgtcgt
    67261 cagtgaaata tgcaggctgc ccgtgggaac tcggtctggt ggaaacccaa caggcgctgg
    67321 ttgccaacgg cttgcgtcat aaaattcgtt tgcaggttga tggcggcctg aaaacgggtg
    67381 tcgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccaa
    67441 tggtggcgct cggctgtaaa tatctgcgta tttgccacct gaacaactgc gccacgggtg
    67501 tagcaactca ggatgacaaa ctgcgtaaga atcactatca cggcctgccg ttcaaggtga
    67561 ctaattactt tgagtttatc gcccgtgaaa cccgcgagct gatggcacag ctgggagtaa
    67621 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agagctggac ggtttcaccg
    67681 ccaaacagca gaaactggcg ctgtcgaagc tgctggagac tgccgagcca catgcaggta
    67741 agtcactcta ctgcactgaa aacaacccgc cgtttgataa cggcctactg aacgcgcagt
    67801 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
    67861 tccgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
    67921 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
    67981 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
    68041 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
    68101 tccgcagcca tgaagcaagc attatcggca acacctgcct gtatggcgcg accggtggtc
    68161 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca
    68221 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt attgtctgta
    68281 ttctgggtaa aaccggcgtt aacttcggcg cgggcatgac cggcggcttc gcttacgttc
    68341 tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgaa gtcttaagcg
    68401 tcgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag catgttcagc
    68461 ataccggttc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccacgaaat
    68521 tcgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcac cgtagtcgta
    68581 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
    68641 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgtaaaa
    68701 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc
    68761 gctgtctgtc gtgcggcaac ccatactgcg agtggaaatg cccggtacac aactacatcc
    68821 caaactggct gaagctcgcc aacgaggggc gtatttttga agcggcagaa ctgtcgcatc
    68881 agaccaacac actgccggaa gtatgcggac gtgtctgccc gcaagaccgt ctgtgcgaag
    68941 gttcctgcac cctgaacgat gagttcggtg cggtgaccat cggcaacatt gagcgttata
    69001 tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg aagcaaaccg
    69061 gtaaaaaagt ggcgattatc ggtgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga
    69121 cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc gggctgctga
    69181 ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct
    69241 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtacagctgg
    69301 acgatctgct gagtgattac gatgccgtgt tccttggcgt tgggacttat cagtcaatgc
    69361 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
    69421 ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg
    69481 aaggcaaacg cgtggtagta cttggcggtg gcgacactgc gatggactgc gtgcgcacgt
    69541 ctgtacgcca gggggcaaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
    69601 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
    69661 tccagccact ggggattgaa gtgaacggta acggcaaagt cagcggcgta aaaatggtac
    69721 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt
    69781 ccgaacatat cgttccggca gatgcggtga tcatggcgtt tggtttccgt ccacacaaca
    69841 tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgctccgg
    69901 aaggcaacga caacactttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
    69961 tccgtggttc cgatctggtc gttaccgcca tcgccgaagg tcgcaaagcg gcagacggca
    70021 ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga aaagagtcgg
    70081 cgctgaaatt ctcctttctt tctggaaatc gcctcgcata atctgaagaa aaccaatgta
    70141 atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc cattattcaa
    70201 cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc tcaacaggct
    70261 accagcaaat ccacaacggc attattcatc tggtcgatag cgcccgaacg gaaacggtac
    70321 gtagcgttaa cgcgctgatg accgcgacgt actgggaaat tggccgacgg attgtcgaat
    70381 ttgaacaagg tggtgaggcc agagcagctt atggtgcaca gttgattgat cggctatcga
    70441 aggatttaag tcgacggtat aaacgtgggg tctctgcaaa aaacttacgc caaatgaggc
    70501 ttttttacct cttttttcaa catgttgaaa ttcgccagac agtgtctggc gaattaacac
    70561 cattgggaat tccgcagaca ccatctgcag aattcccatc ggcaaagatt tggcagacgc
    70621 tgtctgccaa attgagaggc tgtttgatat ttccacagct tttgtattgc atctcctatt
    70681 ttcggtttgt atattcatgc aaataaacga ag
//
LOCUS       DAJTMX010000005        67671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTMX010000005 DAJTMX010000000
VERSION     DAJTMX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.005"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..228
                     /locus_tag="OO145_000364"
     CDS             <1..228
                     /locus_tag="OO145_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5465605.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO145_000365"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO145_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO145_000366"
     CDS             1365..1688
                     /locus_tag="OO145_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5465606.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO145_000367"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO145_000367"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5465607.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO145_000368"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO145_000368"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5465608.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO145_000369"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO145_000369"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5465609.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO145_000370"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO145_000370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5465610.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO145_000371"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO145_000371"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5465611.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO145_000372"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO145_000372"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5465612.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO145_000373"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO145_000373"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5465613.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO145_000374"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO145_000374"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5465614.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO145_000375"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO145_000375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5465615.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO145_000376"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO145_000376"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5465616.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO145_000377"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO145_000377"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5465617.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO145_000378"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO145_000378"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5465618.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO145_000379"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO145_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5465619.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO145_000380"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO145_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5465620.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO145_000381"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO145_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5465621.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO145_000382"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO145_000382"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5465622.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO145_000383"
     CDS             complement(21226..23058)
                     /locus_tag="OO145_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5465623.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO145_000384"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO145_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5465624.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO145_000385"
     CDS             complement(23806..24300)
                     /locus_tag="OO145_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5465625.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO145_000386"
     CDS             complement(24383..24838)
                     /locus_tag="OO145_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5465626.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO145_000387"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO145_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5465627.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO145_000388"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO145_000388"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5465628.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO145_000389"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO145_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5465629.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO145_000390"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO145_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5465630.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO145_000391"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO145_000391"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5465631.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO145_000392"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO145_000392"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5465632.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO145_000393"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO145_000393"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5465633.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO145_000394"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO145_000394"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5465634.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO145_000395"
     CDS             33414..34307
                     /locus_tag="OO145_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5465635.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCTRWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO145_000396"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO145_000396"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5465636.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEDAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO145_000397"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO145_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5465637.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO145_000398"
     CDS             complement(36223..36939)
                     /locus_tag="OO145_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5465638.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO145_000399"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO145_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5465639.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO145_000400"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO145_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5465640.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO145_000401"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO145_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5465641.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO145_000402"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO145_000402"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5465642.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO145_000403"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO145_000403"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5465643.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO145_000404"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO145_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5465644.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO145_000405"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO145_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5465645.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO145_000406"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO145_000406"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5465646.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO145_000407"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO145_000407"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5465647.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO145_000408"
     CDS             complement(45788..46447)
                     /locus_tag="OO145_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5465648.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO145_000409"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO145_000409"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5465649.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO145_000410"
     CDS             complement(47342..48355)
                     /locus_tag="OO145_000410"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5465650.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO145_000411"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO145_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5465651.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO145_000412"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO145_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5465652.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO145_000413"
     CDS             complement(50648..51826)
                     /locus_tag="OO145_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5465653.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO145_000414"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO145_000414"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5465654.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAIRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO145_000415"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO145_000415"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5465655.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO145_000416"
     CDS             complement(55608..55886)
                     /locus_tag="OO145_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5465656.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO145_000417"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO145_000417"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5465657.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO145_000418"
     CDS             complement(56468..57277)
                     /locus_tag="OO145_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5465658.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO145_000419"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO145_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5465659.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO145_000420"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO145_000420"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5465660.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO145_000421"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO145_000421"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5465661.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO145_000422"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO145_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5465662.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO145_000423"
     CDS             complement(60978..61868)
                     /locus_tag="OO145_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5465663.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO145_000424"
     CDS             complement(61855..62379)
                     /locus_tag="OO145_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465664.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO145_000425"
     CDS             complement(62376..62846)
                     /locus_tag="OO145_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465665.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO145_000426"
     CDS             complement(62843..63391)
                     /locus_tag="OO145_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465666.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO145_000427"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO145_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO145_000428"
     CDS             complement(64138..64860)
                     /locus_tag="OO145_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5465667.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO145_000429"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO145_000429"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO145_000430"
     CDS             complement(66023..66778)
                     /locus_tag="OO145_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5465668.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO145_000431"
     CDS             complement(66860..67423)
                     /locus_tag="OO145_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5465669.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16925 a        18281 c        16783 g        15682 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaggaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagt ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcaccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgtcttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgaattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg a
//
LOCUS       DAJTMX010000006        66983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTMX010000006 DAJTMX010000000
VERSION     DAJTMX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.006"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO145_000432"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO145_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5465670.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO145_000433"
     CDS             complement(1151..1237)
                     /locus_tag="OO145_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5465671.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO145_000434"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO145_000434"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5465672.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO145_000435"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO145_000435"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5465673.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO145_000436"
     CDS             3586..3630
                     /locus_tag="OO145_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465674.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO145_000437"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO145_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5465675.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO145_000438"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO145_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5465676.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO145_000439"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO145_000439"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5465677.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO145_000440"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO145_000440"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5465678.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO145_000441"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO145_000441"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5465679.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO145_000442"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO145_000442"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5465680.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO145_000443"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO145_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5465681.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO145_000444"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO145_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5465682.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO145_000445"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO145_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5465683.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO145_000446"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO145_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5465684.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO145_000447"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO145_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5465685.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO145_000448"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO145_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5465686.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO145_000449"
     CDS             complement(16981..17745)
                     /locus_tag="OO145_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5465687.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO145_000450"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO145_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5465688.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO145_000451"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO145_000451"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5465689.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO145_000452"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO145_000452"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5465690.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO145_000453"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO145_000453"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5465691.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO145_000454"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO145_000454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5465692.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO145_000455"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO145_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5465693.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO145_000456"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO145_000456"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5465694.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO145_000457"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO145_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5465695.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO145_000458"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO145_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5465696.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO145_000459"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO145_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5465697.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO145_000460"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO145_000460"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5465698.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO145_000461"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO145_000461"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5465699.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO145_000462"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO145_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5465700.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO145_000463"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO145_000463"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5465701.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO145_000464"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO145_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5465702.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO145_000465"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO145_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5465703.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO145_000466"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO145_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5465704.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO145_000467"
     CDS             complement(40421..41815)
                     /locus_tag="OO145_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5465705.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO145_000468"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO145_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5465706.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO145_000469"
     CDS             complement(42158..43444)
                     /locus_tag="OO145_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5465707.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO145_000470"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO145_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO145_000471"
     CDS             complement(44451..45221)
                     /locus_tag="OO145_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5465708.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO145_000472"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO145_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5465709.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO145_000473"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO145_000473"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5465710.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO145_000474"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO145_000474"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5465711.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO145_000475"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO145_000475"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5465712.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO145_000476"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO145_000476"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5465713.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO145_000477"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO145_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5465714.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLFGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO145_000478"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO145_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5465715.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO145_000479"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO145_000479"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5465716.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO145_000480"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO145_000480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5465717.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO145_000481"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO145_000481"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5465718.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO145_000482"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO145_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5465719.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO145_000483"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="OO145_000483"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5465720.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO145_000484"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="OO145_000484"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5465721.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO145_000485"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="OO145_000485"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5465722.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO145_000486"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="OO145_000486"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5465723.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO145_000487"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="OO145_000487"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5465724.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="OO145_000488"
     CDS             complement(66071..66289)
                     /locus_tag="OO145_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5465725.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="OO145_000489"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="OO145_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5465726.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16246 a        18242 c        17246 g        15249 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcaca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcactagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccag ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgag gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttttcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataatcaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt
    66901 gaggtattaa acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt
    66961 cataaatatc tcctcattag acc
//
LOCUS       DAJTMX010000007        59862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTMX010000007 DAJTMX010000000
VERSION     DAJTMX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.007"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            545..1834
                     /gene="sdaC"
                     /locus_tag="OO145_000490"
     CDS             545..1834
                     /gene="sdaC"
                     /locus_tag="OO145_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5465727.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="OO145_000491"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="OO145_000491"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5465728.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3370..4125
                     /gene="xni"
                     /locus_tag="OO145_000492"
     CDS             3370..4125
                     /gene="xni"
                     /locus_tag="OO145_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5465729.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO145_000493"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO145_000493"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5465730.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO145_000494"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO145_000494"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5465731.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="OO145_000495"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="OO145_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5465732.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="OO145_000496"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="OO145_000496"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5465733.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="OO145_000497"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="OO145_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="OO145_000498"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="OO145_000498"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5465734.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="OO145_000499"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="OO145_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5465735.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO145_000500"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO145_000500"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5465736.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="OO145_000501"
     CDS             complement(13550..13945)
                     /locus_tag="OO145_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5465737.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO145_000502"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO145_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5465738.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="OO145_000503"
     CDS             complement(15232..15459)
                     /locus_tag="OO145_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5465739.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="OO145_000504"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="OO145_000504"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5465740.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="OO145_000505"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="OO145_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5465741.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="OO145_000506"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="OO145_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5465742.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18394..19491)
                     /gene="mltA"
                     /locus_tag="OO145_000507"
     CDS             complement(18394..19491)
                     /gene="mltA"
                     /locus_tag="OO145_000507"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5465743.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19700..19776
                     /locus_tag="OO145_000508"
     tRNA            19700..19776
                     /locus_tag="OO145_000508"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Met,seq:cat)
     gene            19810..19886
                     /locus_tag="OO145_000509"
     tRNA            19810..19886
                     /locus_tag="OO145_000509"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19844..19846,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="OO145_000510"
     CDS             complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="OO145_000510"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5465744.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="OO145_000511"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="OO145_000511"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5465745.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="OO145_000512"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="OO145_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5465746.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="OO145_000513"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="OO145_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5465747.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="OO145_000514"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="OO145_000514"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5465748.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="OO145_000515"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="OO145_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5465749.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34986)
                     /locus_tag="OO145_000516"
     CDS             complement(34645..34986)
                     /locus_tag="OO145_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5465750.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVMIVTALSGIQRTSM
                     NSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREG
                     EMTRLHCPNCQ"
     gene            complement(34971..35378)
                     /locus_tag="OO145_000517"
     CDS             complement(34971..35378)
                     /locus_tag="OO145_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5465751.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35375..35980)
                     /locus_tag="OO145_000518"
     CDS             complement(35375..35980)
                     /locus_tag="OO145_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5465752.1"
                     /translation="MGKTPALSARRNRRRPVKEQGFSLLEVLIAMAISSVLLLGAARF
                     LPALQRESLTSTRKLALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIV
                     QWDANSNGIWDREPVKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTF
                     QVVRQDVSGFSPVLTVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35929..36399)
                     /locus_tag="OO145_000519"
                     /pseudo
     CDS             complement(35929..36399)
                     /locus_tag="OO145_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36583..37377)
                     /gene="thyA"
                     /locus_tag="OO145_000520"
     CDS             complement(36583..37377)
                     /gene="thyA"
                     /locus_tag="OO145_000520"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5465753.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37384..38259)
                     /gene="lgt"
                     /locus_tag="OO145_000521"
     CDS             complement(37384..38259)
                     /gene="lgt"
                     /locus_tag="OO145_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5465754.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38410..40656)
                     /gene="ptsP"
                     /locus_tag="OO145_000522"
     CDS             complement(38410..40656)
                     /gene="ptsP"
                     /locus_tag="OO145_000522"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5465755.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40669..41199)
                     /gene="rppH"
                     /locus_tag="OO145_000523"
     CDS             complement(40669..41199)
                     /gene="rppH"
                     /locus_tag="OO145_000523"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5465756.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41884..42573
                     /gene="mutH"
                     /locus_tag="OO145_000524"
     CDS             41884..42573
                     /gene="mutH"
                     /locus_tag="OO145_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5465757.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42642..43355
                     /locus_tag="OO145_000525"
     CDS             42642..43355
                     /locus_tag="OO145_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5465758.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43493..43711
                     /gene="ygdR"
                     /locus_tag="OO145_000526"
     CDS             43493..43711
                     /gene="ygdR"
                     /locus_tag="OO145_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5465759.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43819..44859
                     /locus_tag="OO145_000527"
     CDS             43819..44859
                     /locus_tag="OO145_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5465760.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44892..46082)
                     /gene="lplT"
                     /locus_tag="OO145_000528"
     CDS             complement(44892..46082)
                     /gene="lplT"
                     /locus_tag="OO145_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5465761.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46075..48234)
                     /gene="aas"
                     /locus_tag="OO145_000529"
     CDS             complement(46075..48234)
                     /gene="aas"
                     /locus_tag="OO145_000529"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5465762.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48820..49851
                     /gene="galR"
                     /locus_tag="OO145_000530"
     CDS             48820..49851
                     /gene="galR"
                     /locus_tag="OO145_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5465763.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49858..51120)
                     /gene="lysA"
                     /locus_tag="OO145_000531"
     CDS             complement(49858..51120)
                     /gene="lysA"
                     /locus_tag="OO145_000531"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5465764.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51242..52177
                     /gene="lysR"
                     /locus_tag="OO145_000532"
     CDS             51242..52177
                     /gene="lysR"
                     /locus_tag="OO145_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5465765.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52164..52856)
                     /gene="ygeA"
                     /locus_tag="OO145_000533"
     CDS             complement(52164..52856)
                     /gene="ygeA"
                     /locus_tag="OO145_000533"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5465766.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52985..54403)
                     /gene="araE"
                     /locus_tag="OO145_000534"
     CDS             complement(52985..54403)
                     /gene="araE"
                     /locus_tag="OO145_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5465767.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54718..55479)
                     /gene="kduD"
                     /locus_tag="OO145_000535"
     CDS             complement(54718..55479)
                     /gene="kduD"
                     /locus_tag="OO145_000535"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5465768.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55509..56345)
                     /gene="kduI"
                     /locus_tag="OO145_000536"
     CDS             complement(55509..56345)
                     /gene="kduI"
                     /locus_tag="OO145_000536"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5465769.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56632..57813)
                     /locus_tag="OO145_000537"
     CDS             complement(56632..57813)
                     /locus_tag="OO145_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5465770.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58068..59297
                     /locus_tag="OO145_000538"
                     /pseudo
     CDS             58068..59297
                     /locus_tag="OO145_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14775 a        16061 c        14581 g        14445 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc
      121 atacaattta tgttgcaaca caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atcgccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacacaggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcaacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcctgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattctaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtttcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatgcc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg ctgcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttagt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccag cgaattcact actgatatca cgatggccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacaac gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatggaacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gattaaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccga tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaaacgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagagctgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg acggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcaa
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tgggattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcccctgt
    18361 catatccgta aaaacggcat acagaatatc acatcagccg ctaaagacgt tacctgcgcc
    18421 cggagccgtt ttcagcaccc aaacccgtcc atagtggttg taccaacctg cgcggtgtcc
    18481 ggcttccggc ccgatccctt gatagatatc gaagtgttgg cctttgattg cgcccccgac
    18541 atccaacgcc accatcagac gcaattcgta ctgaccgtta aatttgccgt tattatccag
    18601 cagcggcact tctgccagca aggtagtacc tggcggaata attgaacgat cggaggcaac
    18661 agaagcacgg ccaaccagcg gcaccgcact tgcccctttc accggtgcaa aagattgcgg
    18721 tttaaagaag acgaaagacg ggttctgttc cagcagctcg cgaacctcgg cttcactgtg
    18781 tgtttcgccc caatgacgaa tcgcctgcat cgacatatct tcttttttca cttcgccacg
    18841 gtcgatcagc accttaccaa tgctgcgata ggcatgaccg tttttccctg catagctgaa
    18901 aaagttaagc ggactgccat caccaaagtc gatataccca ctaccctgca catccataat
    18961 gaagttatcc atcagagagt tgctgtaagc gaggatgtat ttatcgccca atgccccggc
    19021 gtagatctcc gcacgagacg gcagacgacc acgttttggc ggcatacggt aaataggata
    19081 ctggaactcg ccctggcggg tatggcgcgc ctgaattacc ggcgtgtaat agcctgtaaa
    19141 ctgcacgtta ccatagttgt cggcaccttc catctgccag gcatcaatgc cgaactggcg
    19201 catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc accgcgttat aaacattact
    19261 ctggttgcca tacagacgcg gtgacgaatt acggatatgg ttaatttgct cggcaaaatc
    19321 accggcgtta atcggcgcgc caacggcatc tggctggttc accagagaga aaggctgggt
    19381 aaatttcccg tctttatatt gctgtccgcg atcggttggt ttggaagagc aggcggcaag
    19441 cattgccaca accgtgccca taagaaggta ctttacccaa cgtcctttca ttgttctctt
    19501 cttcaggtta aaaaataagg cgcaacgaag ataacaaacc gccgcgttta atgaaatgag
    19561 cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct ttagtggttt gtttttgttc
    19621 aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    19681 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    19741 cgaaggtcgt cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca
    19801 gtacggtgac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19861 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    19921 ctgatctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc ttcctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 aaataatcat gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgctcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgtt caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg attcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgtg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcatgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcaac agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaggcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcggttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaatc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcagatag attgcgcaat ttttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtat ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg agttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagcgcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acaattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gcttttggta ctggtttctg ctggcgagac tgttcattga
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcagcaccat
    34921 cagcaccatc gcaaccatta cctccggcag gctaaagcct tgttgattct tcagggaagc
    34981 tgacataatg ccccctcttt cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca
    35041 atttttccat caatgacttc gcctgttcgc cacaacgaaa cgccttcata accggcaatc
    35101 aataaggctt cattatctgc cagtaaacgc aaacaaacct gggcatcggt tccagcgtac
    35161 tgcgagcact gaactgctgg ctgcgtctgc caggagtgca tttttcccca cgccagcgcc
    35221 gactgaacga tggcctggcg gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa
    35281 ctgcgatcct gctgactcat tccttgcaat agcaagctac ccaaaaccag cagcatcagg
    35341 accagagcca gtgacgaaac acctttttcg cggttcacag gttgaatcct gtcacgctat
    35401 agctggcatc caccacggtt tgcggttcag acttactggc agcacgcata ttaaccgtca
    35461 acaccggcga gaagccgctg acatcctgac gtacgacctg aaaagtgtcg ataatgatgg
    35521 catccggatt agtgacttta tcccagccct taccttcaca ggatgtcgca ccgcgtagcg
    35581 tttccagcac atgctccttc agacgaaatc caatctggtc ggactctttt accggttcgc
    35641 gatcccagat accgttactg ttcgcatccc actgcacaat gatacagtca ccctgtccga
    35701 caatttccag cccttcgccg gtacagctgc catgacaata acccgccctc tggagatgct
    35761 tcgcgacggt aaatacccgc agccagattt catcttccag tgccagctta cgagtgctcg
    35821 ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac
    35881 tgatcgccat agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcctcc
    35941 ttctgtttct ccttgctgat aaagccggag tcttccccac ggcgaaacca ccagccacca
    36001 ctcgcccgtt gagtttttga agcgaatatg cccggcccat gcggtattgc gcaggccaaa
    36061 gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac
    36121 caatggtgaa ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa
    36181 cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg
    36241 taaatagagc aaatagtccc gcgcctagct ggcggtttgc caaagccgtt gcgactgctg
    36301 ccagtattgc cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac
    36361 cagcgtttca atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga
    36421 gaaaaaaaga cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg
    36481 ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa
    36541 accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    36601 tttaacgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa
    36661 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    36721 ttgcagatga gtttgatcca tatggttgct gtacaggtgc gtatcgccac cggtccagac
    36781 aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct
    36841 ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga
    36901 gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt
    36961 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt
    37021 cagctggttc agtaccgtag taatctggtc aatatgacga ccatctggcg ttggccaggc
    37081 gcgccactgt ttaccataca ctggcccgag gtcgccattt tcatcggccc attcgtccca
    37141 gatggtgaca ttgttttcgt gtagataagc aatgttagta tcgccctgca ggaaccacag
    37201 cagttcgtgg atgatggaac gcaggtggca acgtttggtt gtcaccagcg ggaatccatc
    37261 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    37321 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    37381 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    37441 cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc
    37501 ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc
    37561 aatcaggaac aagcctgaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    37621 gataataaac agcaccacac cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg
    37681 cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    37741 atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    37801 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    37861 tggtgcgata aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat
    37921 catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg
    37981 gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat
    38041 acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    38101 ggtccagcca ctgcccggac gattcgcccg acgtgttgcc agccacattg caaaaatgaa
    38161 ccccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    38221 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    38281 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    38341 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    38401 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc
    38461 tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    38521 ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    38581 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    38641 cgccatttca ccgcacaaac gaagatcgat accatgtatt tccgcttccc gggcgatcat
    38701 cgccagagct cgtaacattg caggatgaag actgtcgtaa atgttcgcca cccgggtatt
    38761 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    38821 gaccctcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    38881 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    38941 aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg accatcggca acagaatatt
    39001 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    39061 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    39121 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    39181 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    39241 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacagct
    39301 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    39361 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    39421 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    39481 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    39541 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    39601 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    39661 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    39721 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcac cagcccgctc
    39781 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    39841 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    39901 ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc
    39961 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    40021 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    40081 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    40141 gcggattcgc gtctggcgat attgcccaaa cagggcagtc aactgcgact gagaaagaat
    40201 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    40261 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    40321 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    40381 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    40441 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    40501 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    40561 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    40621 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    40681 acgtcgataa gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    40741 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    40801 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    40861 catattgatt tctgcatcgc cgcttaccag ctgcaagaga aaccattttt gtttttggcc
    40921 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    40981 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    41041 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    41101 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    41161 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    41221 tttatatacg aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca
    41281 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    41341 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    41401 tactacggga aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca
    41461 ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg
    41521 tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtgttta tccacagaat
    41581 gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg
    41641 taaatgaaaa cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg
    41701 tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa
    41761 ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatgtggaa
    41821 aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggtatcat
    41881 gacatgtccc aacctcgccc actgctctct cctcccgaaa ctgaagaaca gttgttagcg
    41941 caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttga cgggctggtt
    42001 acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta
    42061 ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa
    42121 actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgcccgg
    42181 ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc
    42241 gtactgtgga taccggttga aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga
    42301 tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa
    42361 gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggaa
    42421 tatttacaga taagaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc
    42481 cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt
    42541 gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg
    42601 gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa
    42661 ccgatcctaa cgcttggctt gcgctcggta cgctgacgct gctggagatc gttcttggga
    42721 tcgacaatat tattttcctt tctctggtgg tggcaaagct tcccacagca caacgtgctc
    42781 atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa
    42841 tctcttgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttctg
    42901 cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa
    42961 tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg
    43021 gcgctatcgt gcagattatg ctgctggata ttatcttcag cctcgactcg gtgattaccg
    43081 ctgtgggtct gtcagatcac ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg
    43141 tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa
    43201 tgctggcgct ctctttcctg attctggtgg gctttaccct gattctggaa agtttcgaca
    43261 tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc
    43321 tcaacttgat tcgcaacaaa aagaatccgc tctgatactc cgtacgctct cctgcgggag
    43381 ggcgtactca ctccttttac cctcacagtt taagattttc ctgctttcaa gatatataac
    43441 gtcggtttat aaacagacta ttatcatagg taggccaaat gaggataacc ggatgaaaaa
    43501 atgggcagta ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga
    43561 ttacgtcatg gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga
    43621 tgatgatacc gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg
    43681 tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg tcagcatccg gctggcctta
    43741 agatttttct ctttcccttt tcccttccct ctgccatttt tatattcctt atgtcgtgat
    43801 tataaaaagg aaacggctat gcaatatcac cgtatacccc acagttcgct ggaagtcagc
    43861 acgctggggc ttggcacgat gacgtttggt gaacagaaca gcgaagccga cgcccacgca
    43921 caactcgact atgccgtcgc tcagggcatt aaccttatcg acgttgccga aatgtaccca
    43981 gtacctccgc gccccgaaac gcaagggtta accgaaacct acgtcggcaa ctggctggcg
    44041 aaacatggca gccgcgaaaa gttaattatc gcctccaaag tgagcggacc gtcgcgcaat
    44101 aatgacaagg gcatccgccc ggatcaggcg ctggatcgga agaatatccg cgaagcgctg
    44161 catgacagcc tcaagcgcct acagactgat tacctcgatc tttatcaggt gcactggccg
    44221 cagcgcccga ccaactgctt cggcaaactc ggttatagct ggacggattc tgcgcctgcg
    44281 gtttcgctgc tggatacgct ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt
    44341 tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc gctacctgca tctggcggac
    44401 aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt
    44461 tttgaagtag gtctggcaga agtcagccag tatgaagggg tcgaactgct ggcctattcg
    44521 tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca
    44581 cgtaatacgc tctttagtcg gttcacccgc tatagcggtg agcaaacgca aaaagccgtc
    44641 gcggcgtatg ttgatatcgc cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg
    44701 tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac gatggatcag
    44761 ctgaaaacta acatcgaaag tttgcatctg gagttaagcg aagacgtatt agctgaaatt
    44821 gaagcggtac atcaggttta tacttatcct gcaccataaa gattaacgcc ggttaaaacc
    44881 ggcgttaaat attaatgacg gcgctgccag atccacagcg ccgttattgc cagcgcaaat
    44941 agcgcaccga agccaatgcc aatgggcacg accgggatgc ctaccattac cgccagcgag
    45001 taaatgccca gcatcaacaa catggcgctg ttttcgccaa ggttttgtac tgcaatcgca
    45061 ttccccgccc cgacgctttt tttaccccgc tcctgtagca acgcattgag cggaacgaca
    45121 aaaaagcccc ccagcacgcc aatcagcatc aacaaggcat aggctggcag cagctcgtgt
    45181 tgcagggaga aaatcggtac caccacgcca atcaaaatcc cggctggcat acagcgtgac
    45241 acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc caaccacgat accaatcgct
    45301 accatcgcgt tgagataggt cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc
    45361 cacagcacca acaggaaacg cagcgtgaca cccgctcccc agaataaact ggtgcccacc
    45421 agcgaaaaac gcgtttcacc attgcaccat agcgaggtgc aggcattcag gaaactgcgg
    45481 gtcatgttga tgagattcca ggactgcccc ggacgcgccg ccagtttggg aatgtagata
    45541 ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagagcgac caggacatgc
    45601 cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct
    45661 tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc gagaataccg
    45721 tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc gagaaacgga
    45781 ttgataccaa agcagatact ggctgcgccc agcagcttca ggccgttggc aaacatcatc
    45841 acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgccaa aagaatgtaa
    45901 gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa ctgcgctttc
    45961 agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc
    46021 acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac tgactcactc
    46081 atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag gtttgccgct
    46141 gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa gctccggcac
    46201 gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa
    46261 aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat ggactttatc
    46321 tggcgaaaca ccaagtgcca gttgttccac catttccagc gacaccattt cgcctgcaat
    46381 tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac
    46441 aatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct cggcggtggg
    46501 cacttccagt acacctggct tctccacccg cagatagccg ttcattatgt tcggcccttt
    46561 cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgtg catccattcc
    46621 tggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga tagaaacgac
    46681 aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt tatcctgcca
    46741 aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc gtagacgata
    46801 gaagtcatac gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa
    46861 cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg cggtaatgca gcgggcttgg
    46921 ataaaggaac acttctgcac ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa
    46981 ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt
    47041 tttaatctgc tcgacgttcg ccagaatgct tttatggcta tggacgacgc cttttggatg
    47101 gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct gttttacctg
    47161 cgccaggcgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag tggtgacatc
    47221 tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg gcagatgcca
    47281 gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt cagccgccgt
    47341 aatagcactg gtcagccctt ttaccccggc agtgtagttc atcattgcgg gaatgcggcg
    47401 acgcgcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca gcattaagcc
    47461 gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt
    47521 aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt tcttcccggc
    47581 tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc
    47641 catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc gacggtcacg
    47701 ggcacgcggc gcatccggca tcgccacctg cgttggaggc aaaatatgca gagtaatttg
    47761 cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa gttccgcccc
    47821 ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc
    47881 gtagattttc atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg
    47941 tcggccctgt tccaccagac gtaccagatg tttaatagcc ataggttgcg tcgggtcgag
    48001 aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttggc ttattgaggt
    48061 gtaaacggca aacactggac gcacaggtaa aaacagtcca agcaaaatgc catcaataaa
    48121 agagacgtga ttaggcgtaa ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt
    48181 aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa gcattccaac
    48241 tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat aaagaacgcg
    48301 agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga
    48361 cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga tcttgattgt
    48421 ggtctgcacg acgactcttc gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg
    48481 aggtgtgtaa attgtcggtt actgttacag attgatgacc ggcaaaaaaa aacctgcgca
    48541 tctgcgcagg ctggtgtaat tcatgtgctc aacacgaagt tgacttcacc tatcaatacc
    48601 tctgggatca ccactttagc aacctgaagc caaacgccac cagcggtcaa tcgcaacagc
    48661 gtttcgcaaa gtgtaaccaa aggtttgcaa tctctttttt tgtcttactt atttgcgttt
    48721 ggctcacatt cccacgatga aaacacgcca ccccttgaac caacgggcgt tttccgtaac
    48781 actgaaacaa tgtaagcgtt tacccactaa ggtattttca tggcgaccat aaaggatgta
    48841 gcccgactgg caggcgtttc agtcgccacc gtttcccgcg tcattaataa ttcacccaaa
    48901 gccagcgaag cttcccggct ggctgtgcat agtgcaatgg agtctcttag ctatcacccg
    48961 aacgccaacg cccgtgcgct ggcgcagcag accactgaaa cggtcggtct gatcgttggt
    49021 gatgtttccg atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac
    49081 accggtaatt tcttattgat tggcaacggt taccacaacg aacaaaaaga gcgtcaggct
    49141 attgagcaac tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa aatgatccca
    49201 gatgccgatt tagcctcatt aatgaaacaa atgcccggta tggtgctgat caaccgtatc
    49261 ctgcctggct ttgaaaaccg ttgtattgct cttgacgatc gttacggtgc ctggctggca
    49321 acgcgtcatt taattcagca aggtcatacc cgcattggtt atctgtgctc taaccactct
    49381 atttctgacg ccgaagatcg tctgcaaggg tattacgatg cccttgctga aagtggtatt
    49441 ccggccaatg atcggctggt gacatttggc gaaccagacg aaagcggcgg cgaacaggca
    49501 atgaccgagc ttttgggacg aggaagaaat ttcactgcgg tagcctgtta taacgattca
    49561 atggcggcgg gcgcgatggg cgttctcaat gataatggta ttgatgtacc gggtgagatt
    49621 tcgttaattg gctttgatga tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc
    49681 gtgcgttacc caatcgtaac gatggcgacc caggctgccg aactggcttt ggcactggcg
    49741 gataatcgcc ctctcccgga aattactaat gtatttagtc cgacgctggt acgtcgtcat
    49801 tcagtgtcaa ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta accgcagtta
    49861 aagcaattcc agcgccagta attcttcgat ggtctggcga cggcgaatca accgcgcctg
    49921 accattatca aacagaactt ctggtaacag cggacggcta ttgtagttgg atgacattga
    49981 tgcgccatat gcccctgtat catgcagtac cagataatca cctgccttca cttccggcaa
    50041 ggcgcgggtt tcaacatttc ccccttcctg ctgggtaaag acatcgcccg attcacataa
    50101 cggcccggcg acgacggttt ccaccgttgg cgcgtgttcc agagaacgac catcagctgc
    50161 cagggcactg atatggtggt aactaccgta cattgccggg cgcatcagat cgttgaaccc
    50221 ggcatcaacc agcacaaagt ggcggctacc catttgtttg acgctccgca cctgagtaat
    50281 taatacgcca gactgcgcta ccaggaagcg acccggttca atttccagtt tcacagggtg
    50341 gcccaaatgg cgagcgattt gctcacgcgc ggcattccac agaccataat aatgttcggt
    50401 atcaaccgcc tcttcaccct gttgataagg aatagaaagc ccaccgcccg cagaaatagc
    50461 ctgtaaatcc tgaccgaatt cgataacctg acgcaccata gcaccacaca cctgctccag
    50521 atgggcataa tcaacgccag aaccaatgtg catgtgaatg ccgactagct gcagatgatg
    50581 acgttgtatc acgtccagtg cggcgggcag atcggtgtac cagataccgt gcttgctgtt
    50641 ttcgccaccg gtattggttt tttggctatg tccgtgacca aaccccggat taacgcgcag
    50701 ccatacccga tgccctggcg aaacctggcc cagttggtcg agcgtatcaa cagaacccgc
    50761 attcaccgga atttgcaatt cactgacgcg ttcaagcgtc gcctgatcga taacatctgc
    50821 cgtaaaaaca atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc
    50881 tatttcgcct aacgagacgg aatccacttt cacgccctgc tcacgcatta agcgcaaaat
    50941 atgaatattg gaacaggctt tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc
    51001 aatctgccga cgaataattt gcgcatcgta gacccacacc gggcagccaa attcagcggg
    51061 caaacgcagc agattttcgg cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat
    51121 aacaaactcc agatgagtgc ttttttatga ttacgccaca tcataaaaag aataaaaaat
    51181 atcgatttat gtcgagtcta tgcaaaaatg atatggatta ccggattgcg agagagcgct
    51241 aatggccgcc gttaacttac gtcatattga aatttttcat gcggtaatga ccgccggaag
    51301 cctgactgag gcagcacacc tgctacacac ctcacagcca accgtcagcc gcgaactggc
    51361 gcgctttgag aaggtgatcg ggctgaaatt gtttgagcgc gtacgtggac gattacatcc
    51421 taccgtgcaa ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg gactggatcg
    51481 cattgtcagc gccgcagaaa gtctgcgcga gtttcgccag ggagaactgt ctattgcctg
    51541 cctgccggtc ttttcgcaat cttttttacc gcagctcctg caaccctttc tggcacgtta
    51601 tcccgatgtc agcttaaata tcgtgcccca ggaatcaccg ctacttgaag agtggctctc
    51661 ggcccagcgt catgatttag gactcactga gacactccat acgcctacgg gaacagaacg
    51721 taccgaatta ctctctttag atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt
    51781 aaaaaaggta ttaacgccgg atgattttca gggtgagaat tacatcagcc tttcccgtac
    51841 tgacagctat cgccagttgc tggatcagct atttactgaa catcaggtta aacgacgcat
    51901 gatcgtagaa acccacagcg ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat
    51961 ttcggtggtt aacccgctca ccgcactaga ttatgcggca agcggtttag tggtgcggcg
    52021 gttcagtatt gcggttccgt tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc
    52081 agcgctggtt caggcgttta gtgggcattt acaagcgggg ttaccgaaac tggtcacttc
    52141 tcttgacgct attttgtcgt cagctacgac agcataaaag cgacagcatc ctcggcatgg
    52201 atcgccgcgg tatcaaacac aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt
    52261 tctgtgcagc caaaaatgac gccctgtgcg ccctgttctg caaggcgagc aatcacttgc
    52321 gcataataag cgcgtgacgc ttcggtaaat tgccccagac acagttcttc aaaaataatc
    52381 tgattaattt tcgcccgttc atccgcttca ggaataagac aattgatgga aaattgttcc
    52441 gtcagccgcc cgcgataaaa atcctgttcc atggtgtaac gcgtacccag cagcgccaca
    52501 cgagtcattc cggccccggt aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc
    52561 agagtgcaac gtgactcaat ggcatccgcc actttatgca tcgtattggt acataacaca
    52621 ataccttctg cgcccgcccg ctgtaagcca agcgccgcct cagccagaat gtccccggtt
    52681 ttatcccatt ccccgcgacg ctggcactct tctatttcat gaaaatcgac gctatgtagc
    52741 agcatttgcg cagagtgaag cccaccaagc cgctgtttaa tgccttcatt tatcagacga
    52801 tagtaaggaa tggtggattc ccagctcatt cctcccagca aaccaattgt tttcattcct
    52861 ctctcctgat gtgtgttacc gcaacaaggc tgaataagga acgagacaaa cgcctcaacg
    52921 gccaggtgcc caatctctat taacgaaaaa agggccggat gtacagcaca tccggcccgt
    52981 gaaatcagac gccgatattt ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt
    53041 ccagcgtgac atttttggtt tccggaatga gccagaaagt aatacccaca aacgcaatgt
    53101 tcagcgcagt gtagagccag aacgtaccgg cagcgccaat gctatcaagc agtgtcagga
    53161 aggccgcgcc gataatcata ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga
    53221 aatcgcggca tttcagcggc tgaatttcag agcacaggat ccacaccact ggcgcggcgc
    53281 tcatcgcata accggcaata cacatcatcg tcatgccaac agagagccag gacaagccac
    53341 tggaagccgt accgttatca aactgcatca ggcaatagcc cagcaccaga gtgcctaacg
    53401 ccatcacgct gaaaccaatt ttcagagccg gtttacgacc tgctttatct accgtaaaga
    53461 ccgcaatgaa ggtcgcgaac ataaaggtca gtcccacgac cagagtcgca atcatctgtt
    53521 gttctgtggt cgtaaagccc gccattttga agatacgcgg cgcgtagtac atgatgatgt
    53581 tcataccggt aaactgctgc atcgcctgca acaacatacc gagaaacaca gcacgacgga
    53641 cgttacggtt gatcttaaac agtgcccaac cgccctgttt taacttcagg ctttcacgaa
    53701 tttcgttgag ttcttctcgc gctttttccg acgtatcgcg cagcatacgc aacacttctt
    53761 ccgcctcaat atgacgcccc ttttccgcca gccagcgcgg gctatttggc aggaagacca
    53821 ccagaataat cagcagaact gctggtaaag caagaacccc caacattgcg cgccagttac
    53881 cgctataact gaacgctgta tcggataaaa acgccagcac gatgccgagt gtgaccatca
    53941 actggtacat actgatcatc ttaccgcgaa cgttttcact tgccatttca gaaagataca
    54001 gaggagcggt gtaagacgcg atcccgacag caatgcccag caccacacga gcggcgatta
    54061 acatctctac gctggtcgca aaagcggacc ctatagaacc gagtacaaac aggatggccc
    54121 ccgccatcag gctgtattta cgccccaggc ggaacgacag ccaaccatta aacagcgcac
    54181 caattgctgc gccgagcatc atgctactaa ccacccattc ctgcaaacga ctggtcagca
    54241 caaagtgatc ggtaatgaac ggcaacgctc cggcgattac gccgatatca agaccaaata
    54301 acaatcctgc gaccgcagca gctaccgaaa caaacatatt catacgccgc gtatcacgca
    54361 aagaacgtgg cgttaaagca gattccgtat tgatagtaac cattttttcc tgccagcaga
    54421 gagtaagaca ttgtgaaaaa atacgtgaac aattcacaca ggtgtcaggt cggaaacagc
    54481 ataaatatgg attaaattgc tgcgacatgc cgttatgtga tggatattcc aattttaaaa
    54541 ttaagttgaa ttattgagat tattattaac ctcctaattt taaagcagat aaaattcata
    54601 aagttcatta attgataatt gatatggatt atttcataac catgatatgg attatgatga
    54661 tctagagata taaaaaaccc tgccatgcgg cagggtcata aaagtaagag gaatgaatta
    54721 acgcgccagc caaccgccat ccacagcaat ggtataacca tttacataat ctgaagcgct
    54781 ggaggcaaga aacactaccg gccccatcag gtcactcggc agtccccaac ggccagctgg
    54841 aatgcgatcg agaatttccg cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt
    54901 ggtcgccatg taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcgtt
    54961 tgccatcaat cgcgtcacac ccatcacgcc gctttttgat gcggtataag aaggcacacg
    55021 gatcccgccc tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg
    55081 cgcgataaag tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt
    55141 catgacatcg tcccagtctt tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc
    55201 ggcgttattc accaggatat caatatgacc aaattcagct accgcgcgat ccagcagtcc
    55261 aggaatgcca tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc
    55321 cgtgacctgc tcgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc
    55381 cgcttgcgcc agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac
    55441 cgcaacttta ccttggagag aaaatgcact taaaatcata acaacacctt attcgtttat
    55501 gcccacaact agcgcaaatc tttaacgccc acatggtcca tatcattaaa gacctggttt
    55561 tcaccgacca tgccccagat aaaggtataa gctttggttc cgacgccgga atggatcgac
    55621 cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc
    55681 tgccccatca tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc
    55741 atccggcgct cgtgggtgtg acacggcatg gtgttccaca agttacctgg agccagctcc
    55801 gtcagcccca tactcaattg acaggtttcc agtacatccg ggacaaaata tttgttaatc
    55861 gtgcgacggt tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg
    55921 acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga
    55981 gtggcggtat caatactggc aaagacaact tcttttgcac ctttaccaac atacagggcg
    56041 tcgcggtgac cgatttcata gcattgacca tcgacagtaa tcgtaccggc accgccaata
    56101 ttgataacac ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact
    56161 tccccgccaa cggaaaccgt tttagttatc ggcataatgc cgccaacaat aattcggtca
    56221 atgtggctgt aaaccatggt gtactcatcg gcgacaaata ccttttcaac caaaaattca
    56281 ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg
    56341 tccacatcaa cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg
    56401 tttcgttttc tctataaagg gatgatatgc ccatgggcta gggttttcaa ttaaaaataa
    56461 aacaatgttt tatttttaat ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt
    56521 catgagaatg gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta
    56581 ttcaacagat taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc
    56641 acgttcaatg gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagcccttt
    56701 gcgggcatta cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc
    56761 gcctatcggg tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca
    56821 ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag
    56881 atcgacatca gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg
    56941 cgcaattccc atcaatgccg gatctacacc aacgctggca aatgcgcgga tgcgggccag
    57001 cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc
    57061 atcgtttatg gatgatgcat tccccgcggt cacagaaccg agactatcaa atgaaggatt
    57121 taaacgggct aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac
    57181 caacgtctga ccgttacttt ggatcattac cgggacgatc tcatctttaa atcgtccggc
    57241 gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaactgacg
    57301 gctgatgcca tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa
    57361 ggcatcccac aacccatcat gcaccagact gtcaaccaac tggctattgc caagctgttc
    57421 accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccacctgc
    57481 gatgacaata tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag
    57541 accggaaccg caaacgtcat taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt
    57601 aatagccgat tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat
    57661 tacttcatcc accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag
    57721 actaccaagt tccacggcgg aatgacccgc taacgcacca cgaaagcagc cgataggtgt
    57781 ccgtaacgcc ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca
    57841 atagtaaatg attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac
    57901 aaaggaaata tgcctgagca gtagtcagag tcataactgg cacgtaaggt ttgcaaccac
    57961 taacccacca atagatgggt agatagggcg ttaatctccc atacttaact tggtttatgg
    58021 taaattgccc tccattttgt ttaatttgta gatgatacgt tcagataatg tctaatattt
    58081 ggtctaaaga agaaactctg tgaagtttcg cgctctatgg cacagccgtt gatgcaggca
    58141 cgctattcct tcctattcag ttaggttcgg caggggctgt ggtcctgttt attactgctc
    58201 tggtcgcctg gcctttaaca tattggccac ataaagcctt atgccagttc atcctctcat
    58261 caaaaacatc agcaggtgaa gggataacgg gcgcggtaac acactactat ggcaagaaga
    58321 ttggtaatct gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg
    58381 cagtggcaat taccaactca cttacggaac agctggcaaa gcatatggtt attgatcttc
    58441 gcatccgtat gttggtgagt ctgggtgttg tattaattct gaatctcatt tttctgatgg
    58501 gacgtcatgc cactattcgg gtaatgggat ttttggtatt cccattgatt gcctatttct
    58561 tatttctttc catttacctt gtcggtagtt ggcaacctga tctattaaca acccaggtag
    58621 agttcaatca gaataccctt caccagatat ggatatcgat tcccgtgatg gttttcgcct
    58681 ttagccatac gcccattatt tctacgtttg ccatagacag acgtgaaaaa tatggcgaac
    58741 acgctatgga taaatgcaaa aaaattatga aagtcgctta tctcatcatc tgcataagtg
    58801 tactgttctt tgtctttagc tgcctgcttt ctattccatc ttcgtatatt gaagcggcta
    58861 aagaagaagg ggtcaccatt ttatcggcgc tttctatgct gccgaacgcc ccagcatggt
    58921 tgtcaatttc cgggattatt gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt
    58981 actttggcgt tattgaaggt gccacagagg tcgtcaaaac aacactacag caggttggtg
    59041 taaagaaaag tcgtgcattt aaccacgcac tatcaattat gttggtatcg ctgattacct
    59101 tcattgtttg ttgcattaac ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc
    59161 tcattgccat gatacttttc atcatgccta cgctgtcaac gtatctcatc ccggcgctta
    59221 aaccctggcg ttccatcgga aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg
    59281 taatgttctt tagctaatat ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat
    59341 tccatttttc ataatattca tttgttatta ttccgcccta tcacttctct cgtcatatcc
    59401 tagaattatc agttagttaa acacacaaag agatctgaac atcttaaata agactattta
    59461 agatgcataa cttagattcg caagatatct tctggtcatt caaaaacaat ttgcgatata
    59521 gatattctca taccatgaga tagttatctg gaccacgttc gaccatgcaa gattaaatca
    59581 gtgaaacata atcatattta ataacgagaa atgcattttt aaatatctta atcagaatta
    59641 tagcaaatac agattaatcg caatttttcc cagcaaaatt acaaaatcaa ggtgtattga
    59701 catcatcaat caccaatgaa attatgcaat tatatacgga tagggaggtt cttaacatgg
    59761 ggctttgtag tcgttataaa agtcttacat gcaatagttg ctcaatgcat tgccaaataa
    59821 tgccagaaga gtcaccgcgt ttgcagtact gcgcaaactc gt
//
LOCUS       DAJTMX010000008        55418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTMX010000008 DAJTMX010000000
VERSION     DAJTMX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.008"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO145_000539"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO145_000539"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5465771.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO145_000540"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO145_000540"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5465772.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO145_000541"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO145_000541"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5465773.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO145_000542"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO145_000542"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5465774.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO145_000543"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO145_000543"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5465775.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO145_000544"
     CDS             7826..9559
                     /locus_tag="OO145_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5465776.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO145_000545"
     CDS             9708..10559
                     /locus_tag="OO145_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5465777.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO145_000546"
     CDS             complement(10546..10887)
                     /locus_tag="OO145_000546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5465778.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO145_000547"
     CDS             complement(10889..11767)
                     /locus_tag="OO145_000547"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5465779.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO145_000548"
     CDS             complement(11733..14030)
                     /locus_tag="OO145_000548"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5465780.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO145_000549"
     CDS             complement(14081..14401)
                     /locus_tag="OO145_000549"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5465781.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO145_000550"
     CDS             complement(14416..15495)
                     /locus_tag="OO145_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5465782.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO145_000551"
     CDS             15804..16907
                     /locus_tag="OO145_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5465783.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO145_000552"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO145_000552"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5465784.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO145_000553"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO145_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO145_000554"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO145_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO145_000555"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO145_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO145_000556"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO145_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5465785.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO145_000557"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO145_000557"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5465786.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO145_000558"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO145_000558"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5465787.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO145_000559"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO145_000559"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5465788.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO145_000560"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO145_000560"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5465789.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO145_000561"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO145_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5465790.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO145_000562"
     CDS             30120..30728
                     /locus_tag="OO145_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5465791.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO145_000563"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO145_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5465792.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO145_000564"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO145_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5465793.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO145_000565"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO145_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5465794.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO145_000566"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO145_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5465795.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO145_000567"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO145_000567"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5465796.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO145_000568"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO145_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5465797.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO145_000569"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO145_000569"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5465798.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO145_000570"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO145_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5465799.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO145_000571"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO145_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5465800.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO145_000572"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO145_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO145_000573"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO145_000573"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5465801.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO145_000574"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO145_000574"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5465802.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO145_000575"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO145_000575"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5465803.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO145_000576"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO145_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5465804.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO145_000577"
     CDS             complement(44843..45142)
                     /locus_tag="OO145_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5465805.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO145_000578"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO145_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO145_000579"
     CDS             45895..46494
                     /locus_tag="OO145_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5465806.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO145_000580"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO145_000580"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5465807.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO145_000581"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO145_000581"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5465808.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO145_000582"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO145_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5465809.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO145_000583"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO145_000583"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5465810.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO145_000584"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO145_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5465811.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO145_000585"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO145_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5465812.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO145_000586"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO145_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5465813.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO145_000587"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO145_000587"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5465814.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO145_000588"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO145_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5465815.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15018 c        14165 g        12742 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagccag gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAJTMX010000009        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTMX010000009 DAJTMX010000000
VERSION     DAJTMX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.009"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(11..322)
                     /locus_tag="OO145_000589"
     CDS             complement(11..322)
                     /locus_tag="OO145_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5465816.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO145_000590"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO145_000590"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO145_000591"
     CDS             701..1054
                     /locus_tag="OO145_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5465817.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO145_000592"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO145_000592"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5465818.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO145_000593"
     CDS             complement(2810..3280)
                     /locus_tag="OO145_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5465819.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO145_000594"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO145_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO145_000595"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO145_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5465820.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO145_000596"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO145_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5465821.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO145_000597"
     CDS             complement(5283..8432)
                     /locus_tag="OO145_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5465822.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO145_000598"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO145_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5465823.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO145_000599"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO145_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5465824.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO145_000600"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO145_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5465825.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO145_000601"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO145_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5465826.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO145_000602"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO145_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5465827.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO145_000603"
     CDS             14911..15288
                     /locus_tag="OO145_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5465828.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO145_000604"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO145_000604"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5465829.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO145_000605"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO145_000605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5465830.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO145_000605"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO145_000606"
     CDS             18099..18428
                     /locus_tag="OO145_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5465831.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO145_000607"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO145_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5465832.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO145_000608"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO145_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5465833.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO145_000609"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO145_000609"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5465834.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTSEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO145_000610"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO145_000610"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5465835.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO145_000611"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO145_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO145_000612"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO145_000612"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5465836.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO145_000613"
     CDS             complement(25481..27157)
                     /locus_tag="OO145_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5465837.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO145_000614"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO145_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5465838.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO145_000615"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO145_000615"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5465839.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO145_000616"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO145_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5465840.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO145_000617"
     CDS             complement(31205..31999)
                     /locus_tag="OO145_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5465841.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO145_000618"
     CDS             32137..32478
                     /locus_tag="OO145_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5465842.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO145_000619"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO145_000619"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5465843.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO145_000620"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO145_000620"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5465844.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO145_000621"
     CDS             36394..37686
                     /locus_tag="OO145_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5465845.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO145_000622"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO145_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5465846.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO145_000623"
     CDS             complement(38219..38677)
                     /locus_tag="OO145_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5465847.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO145_000624"
     CDS             complement(38674..39591)
                     /locus_tag="OO145_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5465848.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO145_000625"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO145_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5465849.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO145_000626"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO145_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5465850.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO145_000627"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO145_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5465851.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO145_000628"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO145_000628"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5465852.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO145_000629"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO145_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO145_000630"
     CDS             43550..44236
                     /locus_tag="OO145_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5465853.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO145_000631"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO145_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO145_000632"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO145_000632"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5465854.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO145_000633"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO145_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5465855.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OO145_000634"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OO145_000634"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5465856.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OO145_000635"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OO145_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5465857.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OO145_000636"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OO145_000636"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5465858.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="OO145_000637"
     CDS             52939..>53211
                     /locus_tag="OO145_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5465859.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12831 a        13691 c        14016 g        12673 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccagtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAJTMX010000010        52598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTMX010000010 DAJTMX010000000
VERSION     DAJTMX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.010"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..354
                     /locus_tag="OO145_000638"
     CDS             <1..354
                     /locus_tag="OO145_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5465860.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO145_000639"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO145_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5465861.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO145_000640"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO145_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5465862.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO145_000641"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO145_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5465863.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO145_000642"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO145_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5465864.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO145_000643"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO145_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5465865.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO145_000644"
     CDS             6877..7281
                     /locus_tag="OO145_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5465866.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO145_000645"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO145_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5465867.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO145_000646"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO145_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5465868.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO145_000647"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO145_000647"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5465869.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO145_000648"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO145_000648"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5465870.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO145_000649"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO145_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO145_000650"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO145_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5465871.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO145_000651"
     CDS             complement(13133..13429)
                     /locus_tag="OO145_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5465872.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO145_000652"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO145_000652"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5465873.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO145_000653"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO145_000653"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5465874.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO145_000654"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO145_000654"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5465875.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO145_000655"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO145_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5465876.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO145_000656"
     CDS             complement(18686..19372)
                     /locus_tag="OO145_000656"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5465877.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO145_000657"
     CDS             complement(19772..19912)
                     /locus_tag="OO145_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465878.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO145_000658"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO145_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5465879.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO145_000659"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO145_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5465880.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO145_000660"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO145_000660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5465881.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO145_000661"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO145_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5465882.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO145_000662"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO145_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5465883.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO145_000663"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO145_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5465884.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO145_000664"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO145_000664"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5465885.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO145_000665"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO145_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO145_000666"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO145_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5465886.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO145_000667"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO145_000667"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5465887.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO145_000668"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO145_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5465888.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO145_000669"
     CDS             complement(31511..31678)
                     /locus_tag="OO145_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465889.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO145_000670"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO145_000670"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5465890.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO145_000671"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO145_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5465891.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO145_000672"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO145_000672"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5465892.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO145_000673"
     CDS             35470..36114
                     /locus_tag="OO145_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5465893.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO145_000674"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO145_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO145_000675"
     CDS             37189..37452
                     /locus_tag="OO145_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5465894.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO145_000676"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO145_000676"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5465895.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO145_000677"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO145_000677"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5465896.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO145_000678"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO145_000678"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5465897.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO145_000679"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO145_000679"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5465898.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO145_000680"
     CDS             42150..43691
                     /locus_tag="OO145_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5465899.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO145_000681"
     CDS             43663..44526
                     /locus_tag="OO145_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5465900.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO145_000682"
     CDS             complement(44740..45522)
                     /locus_tag="OO145_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5465901.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO145_000683"
     CDS             complement(45519..46592)
                     /locus_tag="OO145_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5465902.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO145_000684"
     CDS             complement(46714..46875)
                     /locus_tag="OO145_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5465903.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO145_000685"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO145_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5465904.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO145_000686"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO145_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5465905.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO145_000687"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO145_000687"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5465906.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO145_000688"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO145_000688"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5465907.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO145_000689"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO145_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5465908.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO145_000690"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO145_000690"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5465909.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12525 a        14236 c        13182 g        12655 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatggt
//
LOCUS       DAJTMX010000011        51363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTMX010000011 DAJTMX010000000
VERSION     DAJTMX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.011"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..316
                     /locus_tag="OO145_000691"
     CDS             <1..316
                     /locus_tag="OO145_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465910.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OO145_000692"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OO145_000692"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5465911.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OO145_000693"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OO145_000693"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5465912.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO145_000694"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO145_000694"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5465913.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OO145_000695"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OO145_000695"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5465914.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OO145_000696"
     CDS             complement(3720..4949)
                     /locus_tag="OO145_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5465915.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OO145_000697"
     CDS             complement(5013..5453)
                     /locus_tag="OO145_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5465916.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OO145_000698"
     CDS             complement(5558..6328)
                     /locus_tag="OO145_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5465917.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OO145_000699"
     CDS             complement(6325..7251)
                     /locus_tag="OO145_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5465918.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OO145_000700"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OO145_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5465919.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO145_000701"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO145_000701"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5465920.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OO145_000702"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OO145_000702"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5465921.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO145_000703"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO145_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5465922.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OO145_000704"
     CDS             12958..13305
                     /locus_tag="OO145_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5465923.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OO145_000705"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OO145_000705"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5465924.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OO145_000706"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OO145_000706"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5465925.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OO145_000707"
     CDS             complement(15125..15487)
                     /locus_tag="OO145_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5465926.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO145_000708"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO145_000708"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5465927.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OO145_000709"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OO145_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO145_000710"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO145_000710"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5465928.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO145_000711"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO145_000711"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5465929.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO145_000712"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO145_000712"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5465930.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO145_000713"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO145_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5465931.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="OO145_000714"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="OO145_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5465932.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO145_000715"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="OO145_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5465933.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO145_000716"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="OO145_000716"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5465934.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="OO145_000717"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="OO145_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5465935.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="OO145_000718"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="OO145_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5465936.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="OO145_000719"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="OO145_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OO145_000720"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OO145_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5465937.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO145_000721"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO145_000721"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5465938.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OO145_000722"
     CDS             complement(36342..36380)
                     /locus_tag="OO145_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465939.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OO145_000723"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OO145_000723"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5465940.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OO145_000724"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OO145_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5465941.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OO145_000725"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OO145_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5465942.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO145_000726"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO145_000726"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5465943.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO145_000727"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO145_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5465944.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO145_000728"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO145_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5465945.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO145_000729"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO145_000729"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5465946.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO145_000730"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO145_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5465947.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO145_000731"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO145_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5465948.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO145_000732"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO145_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5465949.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO145_000733"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO145_000733"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5465950.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO145_000734"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO145_000734"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5465951.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO145_000735"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO145_000735"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5465952.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO145_000736"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO145_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5465953.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..>51363)
                     /gene="murD"
                     /locus_tag="OO145_000737"
     CDS             complement(51105..>51363)
                     /gene="murD"
                     /locus_tag="OO145_000737"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5465954.1"
                     /translation="SPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETMEQAMRL
                     LAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
BASE COUNT        12345 a        14115 c        12603 g        12300 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctatc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gattagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcctgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacctcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct tatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggta attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 cta
//
LOCUS       DAJTMX010000012        48341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTMX010000012 DAJTMX010000000
VERSION     DAJTMX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.012"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            188..481
                     /locus_tag="OO145_000738"
     CDS             188..481
                     /locus_tag="OO145_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5465955.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO145_000739"
     CDS             complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO145_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5465956.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1923..2375)
                     /locus_tag="OO145_000740"
     CDS             complement(1923..2375)
                     /locus_tag="OO145_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5465957.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO145_000741"
                     /gene_synonym="yicO"
     CDS             complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO145_000741"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5465958.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3937..5703
                     /gene="adeD"
                     /locus_tag="OO145_000742"
     CDS             3937..5703
                     /gene="adeD"
                     /locus_tag="OO145_000742"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5465959.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO145_000743"
     CDS             complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO145_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5465960.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO145_000744"
     CDS             complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO145_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5465961.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO145_000745"
     CDS             complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO145_000745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5465962.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO145_000746"
     CDS             complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO145_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5465963.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO145_000747"
     CDS             complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO145_000747"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5465964.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO145_000748"
     CDS             complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO145_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5465965.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO145_000749"
     CDS             complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO145_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5465966.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13525..13614
                     /gene="tisB"
                     /locus_tag="OO145_000750"
     CDS             13525..13614
                     /gene="tisB"
                     /locus_tag="OO145_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5465967.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13894..15078
                     /gene="emrD"
                     /locus_tag="OO145_000751"
     CDS             13894..15078
                     /gene="emrD"
                     /locus_tag="OO145_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5465968.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15086..15583)
                     /locus_tag="OO145_000752"
     CDS             complement(15086..15583)
                     /locus_tag="OO145_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5465969.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15580..15942)
                     /locus_tag="OO145_000753"
     CDS             complement(15580..15942)
                     /locus_tag="OO145_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5465970.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15932..16279)
                     /locus_tag="OO145_000754"
     CDS             complement(15932..16279)
                     /locus_tag="OO145_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5465971.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16388..16801
                     /locus_tag="OO145_000755"
     CDS             16388..16801
                     /locus_tag="OO145_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465972.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16848..18290)
                     /locus_tag="OO145_000756"
                     /pseudo
     CDS             complement(16848..18290)
                     /locus_tag="OO145_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18325..20040)
                     /locus_tag="OO145_000757"
     CDS             complement(18325..20040)
                     /locus_tag="OO145_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5465973.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20207..21100
                     /locus_tag="OO145_000758"
     CDS             20207..21100
                     /locus_tag="OO145_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5465974.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21097..21911)
                     /locus_tag="OO145_000759"
                     /pseudo
     CDS             complement(21097..21911)
                     /locus_tag="OO145_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21911..23527)
                     /locus_tag="OO145_000760"
     CDS             complement(21911..23527)
                     /locus_tag="OO145_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5465975.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23822..24496
                     /locus_tag="OO145_000761"
     CDS             23822..24496
                     /locus_tag="OO145_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5465976.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26205)
                     /locus_tag="OO145_000762"
     CDS             complement(24535..26205)
                     /locus_tag="OO145_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5465977.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGSIGKLYWFMPPSANLALRELGIVLFLSVVG
                     LKSGGDFVNTLVNGEGLSWICYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMT
                     DPPALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26401..26829)
                     /gene="ibpB"
                     /locus_tag="OO145_000763"
     CDS             complement(26401..26829)
                     /gene="ibpB"
                     /locus_tag="OO145_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5465978.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26941..27354)
                     /gene="ibpA"
                     /locus_tag="OO145_000764"
     CDS             complement(26941..27354)
                     /gene="ibpA"
                     /locus_tag="OO145_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5465979.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27348..27420)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27659..27991
                     /locus_tag="OO145_000765"
     CDS             27659..27991
                     /locus_tag="OO145_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5465980.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27993..29231)
                     /locus_tag="OO145_000766"
     CDS             complement(27993..29231)
                     /locus_tag="OO145_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5465981.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPVPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            29332..30432
                     /gene="cbrA"
                     /locus_tag="OO145_000767"
     CDS             29332..30432
                     /gene="cbrA"
                     /locus_tag="OO145_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5465982.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30393..31730)
                     /locus_tag="OO145_000768"
     CDS             complement(30393..31730)
                     /locus_tag="OO145_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5465983.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31805..32953)
                     /gene="dgoD"
                     /locus_tag="OO145_000769"
                     /pseudo
     CDS             complement(31805..32953)
                     /gene="dgoD"
                     /locus_tag="OO145_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32950..33567)
                     /gene="dgoA"
                     /locus_tag="OO145_000770"
     CDS             complement(32950..33567)
                     /gene="dgoA"
                     /locus_tag="OO145_000770"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5465984.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33551..34429)
                     /gene="dgoK"
                     /locus_tag="OO145_000771"
     CDS             complement(33551..34429)
                     /gene="dgoK"
                     /locus_tag="OO145_000771"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5465985.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34426..35115)
                     /gene="dgoR"
                     /locus_tag="OO145_000772"
     CDS             complement(34426..35115)
                     /gene="dgoR"
                     /locus_tag="OO145_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5465986.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35393..36049
                     /locus_tag="OO145_000773"
     CDS             35393..36049
                     /locus_tag="OO145_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465987.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36095..36907)
                     /gene="yidA"
                     /locus_tag="OO145_000774"
     CDS             complement(36095..36907)
                     /gene="yidA"
                     /locus_tag="OO145_000774"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5465988.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37021..37419)
                     /locus_tag="OO145_000775"
     CDS             complement(37021..37419)
                     /locus_tag="OO145_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5465989.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37659..40073)
                     /gene="gyrB"
                     /locus_tag="OO145_000776"
     CDS             complement(37659..40073)
                     /gene="gyrB"
                     /locus_tag="OO145_000776"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5465990.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40102..41175)
                     /gene="recF"
                     /locus_tag="OO145_000777"
     CDS             complement(40102..41175)
                     /gene="recF"
                     /locus_tag="OO145_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5465991.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41175..42275)
                     /gene="dnaN"
                     /locus_tag="OO145_000778"
     CDS             complement(41175..42275)
                     /gene="dnaN"
                     /locus_tag="OO145_000778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5465992.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42280..43683)
                     /gene="dnaA"
                     /locus_tag="OO145_000779"
     CDS             complement(42280..43683)
                     /gene="dnaA"
                     /locus_tag="OO145_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5465993.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43980..44060
                     /locus_tag="OO145_000780"
     CDS             43980..44060
                     /locus_tag="OO145_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465994.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44290..44430
                     /gene="rpmH"
                     /locus_tag="OO145_000781"
     CDS             44290..44430
                     /gene="rpmH"
                     /locus_tag="OO145_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5465995.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44447..44806
                     /gene="rnpA"
                     /locus_tag="OO145_000782"
     CDS             44447..44806
                     /gene="rnpA"
                     /locus_tag="OO145_000782"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5465996.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44770..45027
                     /gene="yidD"
                     /locus_tag="OO145_000783"
     CDS             44770..45027
                     /gene="yidD"
                     /locus_tag="OO145_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5465997.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45030..46676
                     /gene="yidC"
                     /locus_tag="OO145_000784"
     CDS             45030..46676
                     /gene="yidC"
                     /locus_tag="OO145_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5465998.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46782..48146
                     /gene="mnmE"
                     /locus_tag="OO145_000785"
     CDS             46782..48146
                     /gene="mnmE"
                     /locus_tag="OO145_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5465999.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11989 a        13066 c        12222 g        11064 t
ORIGIN      
        1 agcggtaagg tcgctgcggt ttaaaaaacg aaacgctatc gataagaata aaaaggaata
       61 aaagtggaat ataaagtctg gcacttctta ctgaccacgc aagcgcgttt cgtacagcat
      121 gatgagagcg atgaaagcaa actacactta tgcttcattc gatacacatt tgtaaaaggt
      181 tgaatccatg aaaccaacga cactactgct tattttcact tttttcgcta tgccaggcat
      241 cgtatacgcc gagtcgcctt ttagctcact gcagtccgca aaagagaaaa ccacggtatt
      301 acaagattta cgtaaaattt gcacgccaca tgcgtcatta tcagatgaag cgtgggaaaa
      361 gttaatgttg tcagatgaaa acaataaaca acatatacgc gaggccatcg tggcgatgga
      421 acgtaataat cagagcaatt attgggaagc gttgggtaag gtagaatgcc cggatatgta
      481 agaacagcaa caactactga ccaacgcttc tttacctgat atcaggattt cttcattttc
      541 acctttgcag taaccaacaa tgcagtcagc aacatcaatg tgccggacaa catcaacggc
      601 gaagtcagac caatattatc cagcgcataa ccgccgattg ccgcgccaca ggtattagca
      661 agctgaataa ccgccacctg aatagacccg gctttttctg cctgatcggc cagcgagcgg
      721 gtgatccacg ttgaccagcc gacgggaacc aatgcaaaag ttagccccca gataatcgcc
      781 acgccggtag caacgatttt atcgcttccc cacaacgtca gtaccaacgc actcacagcc
      841 agtattaacg gcgcgcctgc taaggccagt tttaccgaac gtttaagaat gaacgacgaa
      901 agcgacgtac caataaagct ggcgatacca aaactcaaca gcaccagcgt taagccatcc
      961 acgccgaatc ccgccaggtt catatacacc gggcgaatat acgtgaagaa agcaaactgc
     1021 ccggcgaaag acatgaagat ggcgatcatc cctgccatca cacccggacg ttgtaataag
     1081 cggaaagtat tttgtttctg atgcgagggt tcgcctggca gtgaaggcaa tgatttgata
     1141 atccagaaaa tacacagcac gcccatcacc gccgccgcat taaagacatt gcgccaaccg
     1201 ataagctcgc ctaaaaaact gcccaacggc gcggcaatca ccagcgcaat agaaaccgcg
     1261 ccgaagatca ccgacagcgc cttcggcacc gtacgcggcg gtaccagacg catggtcagc
     1321 gacgccgaca tcgcccagaa cccgcccagc gccagcccca gacaggcacg accgattaaa
     1381 agcaaactga atgagttagc aaaggaaacc agcaagcagg agagcgtcag caaaacggca
     1441 aacaaaataa caacgtagcg gcggtcagtg gcctgaattg tctgggtaat aaacaaactg
     1501 gcaaacattg ccacaaaggc ggtcacggtc accgattgcc cggcaacccc ttccgaaatg
     1561 cctaaatcct gggccattgg cgtcaacaaa ctgacgggca aaaactcaac gataatcaga
     1621 caggcgacac aaaacgccac cgagaaaacg gctgaccagt tcggtcgggt gatggcatcc
     1681 gcgccgcggt tttcggcaat aaattcactc atggtgttac ccgtggcatg tttcagggga
     1741 aaagccgacc actttaacat tgaaatgtgt gacgaattta acgtttttgc aacttttacg
     1801 gtgttgcttg agctggagtt aagatcgaaa cggaacaggg caaagattac cggatgcggc
     1861 gtgaacgtct tatccacccg acaggtcagg cgcggtctgt aggctgataa gacgaaacgg
     1921 tttcaatgca accagtgaac gccctcggca ggctgaaaga acgcgttata gcgcatctga
     1981 aaagcattaa tatcctgcat ttcaggttcg gtttcacgca gaaaacccag cgccagcctg
     2041 acctgagcat cggtaatcgg cgcggaaagg cgggcttttt gtaaaatccg gtgccattgc
     2101 agcaggttct gttcgctatc atcgacaatg acaatttgcc agataagcaa cacctgcgcg
     2161 gcgttttgca ggtgcgactc atccgggcga tgcaaataac gtaaaaattc gtcaccggct
     2221 gactttccca tttgatcgac catcgactcc acgggtacga catcgatatt cagccgatcg
     2281 ctgagacggc gaatcgcttt tctggccccg gatgtcagca cccgaaaacc aaccacaaac
     2341 actaccgcca gcgtggcgag cattatccag atcatgagtt ctcctgcgta atcagaactc
     2401 atgatatctg gaaaccctcg ccagttacta atccaccaga ataatcttca atgcgaacag
     2461 agctgcgacc accaccacac acaggttcag atcgcgccag cgcccggtgc acactttcat
     2521 gatgcagtac gacataaagc cgagtgcaat cccttcggtg atcgagaaag taaagggcat
     2581 catcaccgtg gtaataaacg caggcaccga ttcggtaaaa tcatcccagt taacgcgcgc
     2641 caggctcgaa gtcatcagca cgccaacaaa gattaacgct ccagcggttg cgtaaggagg
     2701 aaccatcgcc accagcggtg agaagaacat aactaacagg aacataacgc cgaccacgac
     2761 agccgttagc cccgtgcgac caccgactgc cacaccagaa gtactttcaa tataggcagt
     2821 aacagacgag gtaccgataa atgcgcctgc caccgaactg acgctatcaa catacagcgc
     2881 cttattcata ttggggaatt taccgttgct atctattaag cccgctttat cagttacacc
     2941 aattaatgtt cctgatgaat caaataggtt gatcagcata aaggagaaaa tgataccggc
     3001 gagttcaagt gttaacgcgc cgctcaaatc tacttcacca atgacgccgc taatatcagg
     3061 cggaatggaa tagacgccgc taaaatgaac atcaccgaaa aataatccac agcaagacgt
     3121 caccacaata gaaaccagca ccgcggcatg aaaatgacgt gatgacaaca cggttataat
     3181 aaaaaaccct aaaataccta acaacacgcc gtgagaactt aaatcgccaa tcatcaccag
     3241 cgtgtcttta ttggcgacaa taacaccagt gttttttaat cccattaagg caataaataa
     3301 tccgattcca ctggtgatac caatacgtaa acttaatgga atgttggaga tcatccagta
     3361 ccggatacga aacagcgtga gcaaaaatag cccaattgcg ccccagaata tcgcgcccat
     3421 cccggtctgc caggagatcc ccatcgcccc cacgaccacg aacgcaaaga aggcgttcag
     3481 ccccattgcc ggagccagcg ccacgggtaa gttagcaaat atccccatcg caatactgcc
     3541 gataccggca atcaaacagg tggtaacaaa caccactttc gggtccattt gtgccgcgcc
     3601 gaggatttgc gggttcacaa aaacaatgta caccatggtt aaaaaagtgg tcatccccgc
     3661 aatcaattct gtgcggacgg tggtcccatg ctgaggtagt ttaaataatc gcgaaagcgt
     3721 ccctgattca ttactcacgt aatcggtatt gtcattattc atttttttgt ccattggagg
     3781 agatttaatc ccttgctttt gtaaaacaaa tgacaacaac ggttcagtga taattatcac
     3841 atttcaattg cacattaata gatattcttt aataatctcg cgacgtttct ttatgataaa
     3901 taataatcaa attgataaaa tcaaaatgag aaaaatatga ataattccat taaccataaa
     3961 tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg tggcgacgct
     4021 gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgc cggagaaatt
     4081 tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga atacgctgat
     4141 actccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat
     4201 gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac cgctaccctg
     4261 ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa
     4321 gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag
     4381 gtcagctctt gcgtgcccgc tctggaaggc tgcgatgtta acggtgccag ttttaccctt
     4441 gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga aatgatggac
     4501 taccctggcg taattaacgg gcagaatgcg ctgctcgata aactcgatgc atttcgctac
     4561 ctgacgctgg acggtcactg tccgggtttg ggtggcaaag aacttaacgc ctatattgct
     4621 gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg gaaattacaa
     4681 ctcggcatgt cgttgatgat ccgcgaaggg tccgctgcca gcaatctcaa cgcactggca
     4741 ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg
     4801 tgggagatcg cccatgaagg gcatatcgat gccttaattc gccgcctgat cgaacaacac
     4861 aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg ccactttggt
     4921 ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct gttgagcgat
     4981 gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg aaccgattga tgcgcaaacc
     5041 ttacaggcgg aagagtcggc gagactggcg ctgtctgccc cgccatatgg caataccatt
     5101 gcccgccagc cagtttccgc cagcgacttt gccctgcaat tcacgcccgg aaaacgctat
     5161 cgggtcattg acgtcatcca taacgaattg attacgcact cccgctccag cgtctacagc
     5221 gaaaatggtt ttgatcgcga tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa
     5281 cggctggctc cggcctgtgg tttgctcggc ggctttggcc tgaatgaagg cgcgctggct
     5341 gcgacagtca gccatgacag ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg
     5401 gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc
     5461 caagtacaaa gtcatctgcc gttacccatt gccgggctga tgagcaccga cacggcgcag
     5521 tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc
     5581 gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc gctaaaacta
     5641 accagtcagg ggctatttga tggcgagaag tttgccttca ctacgctgga agtcacggaa
     5701 taataaaaaa agcccggcgt catgccgggc aaaagtcacc agttacgttt atgccactgt
     5761 caactgctga atttttttct cgcggcggat tttgcgttct tccattaccg ccactatcgc
     5821 catcaggcag atacaaccaa tcgcggcgat atccagcgcg gcgaaggtgc ctgcccagcc
     5881 ggtaaggccg aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct
     5941 gtcaccaatc aggtaagcaa aggtgccttt aataccatcg gcagcgccaa tcgctttttt
     6001 aggtacaaag ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc
     6061 caacgcaaag agagaagcca gatagatata ttcgttactg gcatgttgat acacaccgag
     6121 cgtggcgata atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgccag
     6181 gtcagagagc cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa
     6241 gccctgaatc gccaccgctt tagagagttt cagttcctgg aacgcgtata cggttgacca
     6301 ctggtcgata ccaatacgta ccacatagag gaaaatgttg gcgaagcaca gcagccagat
     6361 cactttgttt ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc
     6421 tgtctctttg tcctcttcgc tgatctcctc gccgaacagt tcttcagctt tgccgaggcc
     6481 ataagattcc ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat
     6541 aatcgacggg aagataaaca tgccgatgac atggccatcg aacaggtaat ttgccccgaa
     6601 cagcgccaca cctgctgcgc ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag
     6661 gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc
     6721 gccggtactc tggaaaaagc cgcttaaggc gtagaaggca atcatcagga acaggctaac
     6781 cgagccgctg cccatactgg cactgaagcc cagcatacaa atggcagaga ggataagcat
     6841 gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag tatgaaacca acgttttacc
     6901 cacgccataa gtgatggaga aacccaggcc gatcatcccc agctgcgtca tgctcaaccc
     6961 gtaggtcgaa atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt
     7021 caggtagccg ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg
     7081 ccgcacttcg agcggaaggt ccagggtcgg cttgcgaact tggtttagga aagccagcat
     7141 gggttactcc tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc
     7201 aggcctgaga tagcgtccaa gaaaaacctg gaaaatttct tagttttgcc ggatatgaag
     7261 tgaaaaggtg agatgcatca cgcttcacgc ggtgtctggg cgttcaaaaa gggcagtaac
     7321 agcagtgcgg aaatcccggc ggcgatagag ataaccacaa aaaatccgct ccagtgccag
     7381 gtatcgagca ctttcgccag cggccaacca gcaagcgacg cccccagata agcaaacaag
     7441 ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccata
     7501 ccaattaaca tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc
     7561 atcacgtagc tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg
     7621 gcgaaaatca aattcatcgg cccacggttg ccgttaaaca atttgtccga gccccaaccg
     7681 gctaccagcg caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg
     7741 accagatcga cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gatcgccgcc
     7801 cggaccacat agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac
     7861 acatatttgg tgaggatctc tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt
     7921 tccagcgcgt cgtgccgcca ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg
     7981 cgtagccgcc agcagagaaa aatccccacg actatcgcca tacaaccagc aatcatcatc
     8041 ccggcacgcc agccgtaatg cagcgcgctc gctgccatca caatgggaat gagtgcgccg
     8101 ccgacgttat gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgtgaatac
     8161 caggccgtta acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc
     8221 acccagagca cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttcatgatg
     8281 cccgtggcga taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg
     8341 ccggagacaa acttcgacac gccgtaagta atgtaaaaca gggtcgctaa caggccgata
     8401 tcgctacggc tgagcacgcc gttagcaagg atttctggta ccgcggcgtt aaaacttttc
     8461 cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag
     8521 tagcgataac gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct
     8581 ttcagaaacg gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca
     8641 cgcgtgccgt gcagacagga aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc
     8701 attccggtga ggccaaaacc ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct
     8761 tcaatcacca gcatcaaccg ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca
     8821 tcggcatgtt tcacaatgtt gttcagccct tcctggcaga cacgaaacag cgtcacgcgc
     8881 tggttttcgc ttaatgctga ttcatcgata cgccattcga gatggctgac aatgccgcgc
     8941 ccttccagct ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc
     9001 agctggcgcg gacgtaaccg tcctaacaaa cggcgcaccg cgtcgtaaac gcccagcgac
     9061 agttgttcga tgagctgccc gctctgcttc acactggcgt tatctgccgc cagccgctga
     9121 acaatgcccg cctgagtacg aatagcagtg atggtctgac cgatatcgtc gtgcagctca
     9181 cgcgccacgt cgcggcgcac gctctcttcg gtttctaaca aacgttcagc cagatgctga
     9241 ttgcgcgcca gttccttttg tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg
     9301 cccagtaaca accctgtcag actttgcacc agcaacgaga gtaataaatc caccagatga
     9361 tcgcgccagg tttgactggc gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct
     9421 ccttgccagc catagtgcca ggccagcgcg ataatcggca gcgccagaca gaatggcgta
     9481 aagcgcgaca gttcgtccgg caatcccaac tggagccaga gactgataac aaacagcagc
     9541 aagtaccaga ccagatgccg cccgcgccag ttgatgggct gagaaaccag tgacgggccg
     9601 agcggcagcc aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc
     9661 gtcagaccgc cagttaaagt cagcaacagc gcattccacg actctttgcc gtgccaaagc
     9721 cagggcagcg actgcaacaa cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg
     9781 cgccagtcac gctgatggcg atagcgcgag atcagcgcta cgggcagtaa cgtcagcaaa
     9841 ctaccgatca tcaataacgg aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc
     9901 agcagccact ccgcacccag caatacgggc cagtatccgc gcgggcattg cagcattagc
     9961 cccagacgca gaccaaacgg aaataacagc accgccatat cagggcgctc aaccagatgc
    10021 aggctgatac tccataggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg
    10081 gtaattaagc gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagct
    10141 ctacgtcgtt actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt
    10201 tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt
    10261 ccgccacctg gcgttcacgt ttggtcagcg ggtcctgacg accggatgcc agtttaatgg
    10321 caatatccgg cgtcagataa cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt
    10381 catccgggct acagcgtttg gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa
    10441 ccagcgcagg actgtcgtga acggagagca taatcgtcgc catacctttc ggcagctggc
    10501 ttagcagctc cagaccggag atatcgggca tggagatatc gcaaatacac acctgcacac
    10561 cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacctgca
    10621 aatcaggttc cagccccagc agctgcgcaa agccggagcg gacgatgagg tgatcgtcta
    10681 taagggcaac ggtgatcatg gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa
    10741 cgataagcgc gatgttgttc aagccttgag cggttactga aaaaacaccg cgatcttgtt
    10801 aaacatcgtc ggatcggact gattacgctg cactttcacg acatcttcca gcttagcgat
    10861 ttggcttatc atctgctcca gacgctggtc gtcattgacc agtagccaga tatggctttt
    10921 gtcgctgtcc tgaatcggca gacaaagaat gccttcaacg ttaaaagcgc ggcgggcaaa
    10981 aaggccacaa acgtgggtca ttacgcccgg atggttgcga acggtgagct ccagaattac
    11041 gttgttatga gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc
    11101 tggcggcacc atcgggtaaa ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg
    11161 gccagggcga ttgatgattt cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc
    11221 acaggtttcg aggccgaatc cggcggcaat ctgcataaag ttgattttgc ccggataggt
    11281 ggcggcaaaa acgccttgct cgtagaacag actctgttgc tgatgcacca gccccagcgc
    11341 ttcgttgttc atcagaatga ttttgacatc cagctgattt tcactggcgg tcgccatctc
    11401 ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt
    11461 cgccagcgcc gcgccaatcg ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga
    11521 ggtcagccac tggcgtgggc gattgagcgg ataagcttgc gcggtccaca tctgatgctg
    11581 acccacatcg gtggtgataa tcgcattgtc atcgacacag gcggcaacgg cgttgatcag
    11641 gccgtaatgg cttaatggat cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa
    11701 atccgctacc aactggtgcc actctgcacg cggttgcgct tccacccgcg ggatcaattg
    11761 cgccagcacg tcatcaacat ccgcctgaat cgccacatgc ggctgcttga ttttacccag
    11821 ctctgcgcga tcgatatcga catgaataat tttggcattc ggacagaact gctcggtttt
    11881 gccaatcgcc cggtcatcaa aacgcgcacc gagcacaatc aacaaatccg cctcctgcaa
    11941 aatatagttg gtactgcgca cgccgtgcat ccccagcata cccagcgaca acggatgcgc
    12001 ttttggcagc atgcccagcg ccattaaagt catggtggta ggcagttgcg ctttctccgc
    12061 cagttcacgc acccgcgcag gcgcattgat cacaccacca cccagataaa gcaccgggcg
    12121 tttggcagcg ttaatcattg tagctgcgtc acgaatgctt tcttcgctaa aggcgggtgc
    12181 aacggctttt tctgccaccg cgggctgagc ttcaatctca aaaaccgccg tttgtacatc
    12241 cttaggaatg tctatccaca ccgggctagg gcggcctgat tgcgcaatgc ggaaggcatc
    12301 gctcatgacc tgtgggagct cttcgatatg tctgaccaga tagttgtgtt tggtgatggg
    12361 gatagagatg ccgtaggtgt ccacttcttg gaaggcgtcg gtgccgatca tcgaggcggg
    12421 aacctgacca gtgatgcaaa tcagcgggat ggagtccagc cgcgcatcag caatggcggt
    12481 caccaggtta gtcgcacccg gcccgctaca ggccatacag accgccggtt taccgtcggt
    12541 gcgcgccatt ccctgagcga taaaccccgc gccctgttca tggcgagcca gaatatggcg
    12601 gatttgcgtg ctttggctta aggcgtcgta aacaggcagg atagaaccgc ccggaatgcc
    12661 cgtcacaatc ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa
    12721 gcgcttacgc gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc
    12781 gactttccgg gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg
    12841 aatcgtgtgt tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac
    12901 gaccactgcg gcggatggcg cagttggtag tagttttgcg ttgaacatgg aagtagtcat
    12961 tggggacctt gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag
    13021 acaatggaaa atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt
    13081 gcagataaca aaaaaccccg ccggagcgag gtttcgtcag ccgcctgcgg ctggtaaccg
    13141 caaagcacac tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat
    13201 ttcggcacga attttgacgt atttagtgca tagttgagta tcgttcacag tttgcgtttt
    13261 gtccaaatat tactgtttat ttatacagta agcttctata atatcactgt acgcaatgtg
    13321 ttatgcgggg gccgcatcgt tacccggcgc actaagtcct ggctgaaacg ggtggtgccg
    13381 tcagcgcctt aaccccgcgt gagcacactg tgttatgtca acaagcacaa cgtttctcct
    13441 tgagataccg cgtgcacaac agctggcaac aggcagcgga aaggtacgtc agctggcagt
    13501 gctcctgaac cacaggagac gcgtatgagc ctggtggata tcgccattct tatcctcaaa
    13561 ctcattgttg cagcactgca actgcttgat gctgttctga aatacctgaa gtaattcaga
    13621 ttcaagtcgc accaaagggg agcgggtaac cgctcccctt ttatatttag cgtgcgggtt
    13681 ggagtcggat gcgatgctga cgcatcttat ccgccctacc atccctcccg gcaacattta
    13741 ttgccgcttt tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg
    13801 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
    13861 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
    13921 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
    13981 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
    14041 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat atccgaccgt
    14101 gtcggtcgcc gaccagtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
    14161 gcggtcacga cctccagttt gacggtattg attgccgcca gcgcgatgca ggggatgggc
    14221 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgtg atttatatga acggacacag
    14281 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
    14341 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
    14401 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
    14461 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
    14521 aactgttatt tgctgatgct gattggcggt ctggccggga ttgctgcctt tgaagcctgc
    14581 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
    14641 attctgccga ttccggcggc attttttggg gcatggtttg ccgggcgtcc taataaacgc
    14701 ttctcaacgt tgatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
    14761 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
    14821 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
    14881 ttcctggcgg gcacggctgg tgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
    14941 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
    15001 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
    15061 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
    15121 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
    15181 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg ccgagcgtgc gggcacaggc
    15241 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
    15301 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
    15361 actcaaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
    15421 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
    15481 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagct
    15541 tatgcctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
    15601 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
    15661 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
    15721 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgaggatgcc
    15781 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
    15841 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
    15901 ccctgggtcg gcaatgcgtc tggctttacg gctatccggc atacaacacc agtcccataa
    15961 cgatcaccgc cacaaccatc aatattaagc tgatgattaa taagctgttg gtgtagggca
    16021 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacattg
    16081 ctaacccgcc ggaaaacaga cacaacaaca acgccagcag ttcacgaatg acgggcgtgg
    16141 cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
    16201 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
    16261 caaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
    16321 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc cgctacaagg
    16381 aggtcagatg ggtatcatag cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc
    16441 catcgcttta atgatgggga ttatccactt ttcattcggc cctttttccg caccaccgcc
    16501 aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg
    16561 aataaaaggc gaaaaaatta ccactgtgga gaaaaaagag gacatggata tcgataaaat
    16621 cctcgatcaa agcggtatcg cactggccat tgctgcgcta ctttgcgcat ttattggcgg
    16681 tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg gtttttggta tcggcatcgt
    16741 gtgcagcatt ttgctgatat tcctgatttt ttccttcctg accggcggtt cgctggttta
    16801 agcagcctga ataatgcccg ccgggtgatc atcagcgggc attatcgtca gaatttctgt
    16861 tttttctcct ccaccttcac tccttgcgtc ggtaggccgg tgtcatagtc gcgtaccact
    16921 ggcgaatagc catcttgtgg gcgtgggcga aacgccccca tccagcgcgg tcgtgcatct
    16981 ttacgccacg gacgcagact ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg
    17041 taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc
    17101 aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg
    17161 ttgagcacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag
    17221 ctgtcatgct caatctcgta gcggttaaat tccaccatca cgccgcgtgg ctctttcacg
    17281 gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt
    17341 gtcggcagta aatcaatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc
    17401 gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc
    17461 agcttatgtg cgcccatcat ttcaccgtga tcggaggtat aaatgaccca cgtattttca
    17521 cgttgctctg gcgttaaggc attgatgaca cgtccgattt ggtcatcaac aaagtcattg
    17581 caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc
    17641 tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgtga tttctcgcca
    17701 agttcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga
    17761 tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcac gggctattgc
    17821 agaaaatcta ccgctcgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg
    17881 ttcgcctgta aatcttcgac gctgtttagg ccattgcgcc acaggctgat ctctttttcc
    17941 gtcagttcgc taagatagtt cgccccatca aaccagtaat cagcatccca ctccggcgga
    18001 cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg
    18061 tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg
    18121 ttgttggtcc acgggccgga ctggttagcg tagataccgg tgaatagtcc ggcccgtgca
    18181 ggcgtacaaa ccggtgaaca agtgtaggcg gaattaaagc gaataccttc cgccgccaga
    18241 ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgaccat attggtggcc
    18301 tgggtatcgg tcaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac
    18361 ggctgggtcg tgacgatgcc accagctgtc aaaaatcagg tagacaatca ctacggcagc
    18421 gatgaaataa ctgatcatcg ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc
    18481 gacatagacg ccaatcatcg cgaacaggat accaattgac gcgatcttga cgtttttcca
    18541 cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat
    18601 aaaaccgatc accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag
    18661 gaaatggaag tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc
    18721 catcgccact tttgccgcca gcgccgggat gcgcggaaag aaaaagccca taatgatgat
    18781 ggtaaccagc ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg
    18841 cggcgcgttc gcgatccacg gcgctaccaa aaccgaaacg atagcgataa agaaaccaaa
    18901 tttgcgcccg acggtgacca gctgctgcgg ctcggcattc tggttaatga tgcgacggta
    18961 aatacccata ctgaataatg tactggcgct attcagaaag ccgttgaagg tactgatcac
    19021 cgcaccaaat aacaccgcgc cgaagaaacc ctccagtggc actggcagaa cgttattgac
    19081 cagcgtcggg taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa
    19141 ccctggcagc accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc
    19201 cttttgccct tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt
    19261 acaccagtaa aaggtgttca ccagaattaa accggtaaat gccgcgccaa tcggcaaggg
    19321 atcggtcggg ccaccgactg agtttaattt ctcggcgtga acggtggtaa tttgctcaat
    19381 gccctgcata aagctgccct tgcctatcgc gatcaggcca aataccggca ccatcaaccc
    19441 gccgataacc agcccaatac cgttgatgga gtccgccact gccattgcgc gcagtccgcc
    19501 gatcaccgca tacaaaattc ccgccagacc aagcaaaatt accagtagcc agatagccgc
    19561 accgtgagaa atctgtagcg attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc
    19621 ggagtagaga acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat
    19681 gataatacgc gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc
    19741 gcgcttcaga tagcgcggta aaaagattag cgcgaggaag atcagcgtca ccgctgaagt
    19801 cacttcccag cccatcaccg acatgccgct tttgtaggcc tgcccggaaa gaccaaccag
    19861 ttgttccgtg gaaaggttgg ttaacattaa cgaagcggca ataaccggcg cttttagtga
    19921 acgtccggca agaaaatagc cttgttgcga tccggtatct gttttgcgaa ccttccacca
    19981 ggtgatcacc gccaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat
    20041 ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc
    20101 gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt atccctgaac ggattgtttt
    20161 ctgttaagtt caggttgtga gcataattcg ccggagggat attaaaatga atggaaaatt
    20221 gcaaagttca gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa
    20281 tgtgatctcc agcggaattt ctctgatctc gctctggcat acctacgccg acgagcatta
    20341 ccgggtgatc tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt
    20401 gtataccgta caaggatgtg ggaaaattct tttaaagaat ggcgaacaaa taactctgca
    20461 tagcaactgt attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt
    20521 agtctgggaa cagtactgga tggaatttac ccccaccagt atgatggata tttccgttgg
    20581 tcagcaaagc gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga
    20641 gttaataact tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat
    20701 tatttatcag tggatttgtc ttatgtccgc agatggtaaa aaagatccac aacggcggca
    20761 aattgaaaaa ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga
    20821 tatggctgcc acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac
    20881 cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt
    20941 aaagcaacaa ggaaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt
    21001 tcatttcagc aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa
    21061 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
    21121 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
    21181 tttcgacaac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
    21241 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
    21301 gccgaccacc agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc
    21361 ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt
    21421 ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg gttggagtag
    21481 ttgagcatct aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg
    21541 ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaaca
    21601 ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg
    21661 tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac
    21721 gcaatgtccg gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc
    21781 cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc
    21841 aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag
    21901 aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa
    21961 ttaagctatc gagctgttca cgcaattggg atacatgcag accaatgatc acctgaatgg
    22021 catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg
    22081 tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc gcaattgtta atgctggaga
    22141 tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg
    22201 gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca
    22261 cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa
    22321 ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg
    22381 cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctccgc
    22441 ccatgttgcc caccacacca aagagataca ttacggtcga cattgaggcc gccagcacgg
    22501 cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa
    22561 ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact tttacccgat
    22621 tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg
    22681 agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagtggc tcggcgctca
    22741 gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca acagctgccg
    22801 gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt
    22861 cgaggaaggt gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag
    22921 attcaatccc catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca
    22981 tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg agaaaaaccg
    23041 gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg
    23101 cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg
    23161 ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat
    23221 tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca cgtccctgcg
    23281 cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata
    23341 ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg
    23401 tcagtgattc cccgacaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taacaaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tactgccgat
    24961 acgcccgagg atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc
    25021 cgggaatcct ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat
    25081 aaacactggc agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc
    25141 aacggcatca attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct
    25201 gatatcgcca ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc
    25261 atagcgttct ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac
    25321 caccacacgc tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg
    25381 accaatcacc agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc
    25441 gcccagttgg ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg
    25501 cgagcagata attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag
    25561 gttagggttc tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga
    25621 agactcgtgc tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca
    25681 catggtgaac aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat
    25741 ctgatcgacc atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag
    25801 cgctggcgta ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc
    25861 aaacagttta tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa
    25921 caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat
    25981 cccgatggta taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct
    26041 acttaatgtc atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac
    26101 gccgccaata cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac
    26161 tgccaccaaa gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg
    26221 agataacatt ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag
    26281 cgcacattat cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat
    26341 tgttgaagaa gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag
    26401 ttagctattt aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg
    26461 ctcattacga attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc
    26521 catattttca gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag
    26581 ccattttttc tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc
    26641 cagttgaatc tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg
    26701 gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg
    26761 cagtgcgttg gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa
    26821 gttacgcata gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc
    26881 gcatatgctc cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa
    26941 ttagttgatt tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat
    27001 atacagcaaa ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa
    27061 tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc
    27121 gtcggcgtga gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct
    27181 ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac
    27241 gttatacgga gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa
    27301 acggtcaaat ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa
    27361 tagctcctga aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga
    27421 acacccatca ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt
    27481 caagccttat cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc
    27541 agcacgaaac gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc
    27601 agcaagggat cgctataact catcaggcca acacccacga tcaacagatg aatttattat
    27661 gataagaaat gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg
    27721 ctccagcgtg atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag
    27781 cgccaccatg attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga
    27841 tatgccgttt accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga
    27901 cagctcggtg cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt
    27961 tattccaccg gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc
    28021 tggccacctt ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca
    28081 gaaggtggat tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca
    28141 caaacaattc gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat
    28201 gcagactgaa tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag
    28261 ataagccaca gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc
    28321 tgcggattac agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga
    28381 tgggtaaagg ttaaacgtcg ctgcacgacg ccacgcggtg gcacgggtag ggttgtttcc
    28441 gttccactta acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca
    28501 acgataaata gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc
    28561 agcgcgtgat ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc
    28621 tgcggcgtgg gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc
    28681 ggatgttgct tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa
    28741 tccagcgccg gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca
    28801 ccgttcgggc taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc
    28861 gcggtaatat ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt
    28921 cgatgatgga agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca
    28981 tatttctctg acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga
    29041 tagataacct cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac
    29101 gacgccccag aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg
    29161 gtatttgtat tggtattggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
    29221 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
    29281 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
    29341 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
    29401 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
    29461 aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg
    29521 acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc
    29581 gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc
    29641 gacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc
    29701 cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag
    29761 caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga
    29821 catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag
    29881 aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca
    29941 tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt
    30001 cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa
    30061 gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt
    30121 ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg
    30181 gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag
    30241 ccggagaaga gcaacgccgc ttactggcgc gccacacaca aactgcgttt aaaactcttc
    30301 ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc
    30361 agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc
    30421 agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc
    30481 gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga
    30541 ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa
    30601 gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg
    30661 cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc
    30721 aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc
    30781 agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg
    30841 gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac
    30901 caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc
    30961 agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc
    31021 ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct
    31081 ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag
    31141 ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc
    31201 cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca
    31261 acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg
    31321 aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc
    31381 gtggcaaagc cttggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc
    31441 acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc
    31501 caggcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga
    31561 atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata
    31621 aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata
    31681 tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat
    31741 ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca
    31801 ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg
    31861 gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt
    31921 tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt
    31981 cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttactga agtaccgcgt
    32041 tataggaaac aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt
    32101 gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa
    32161 taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc
    32221 gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg
    32281 ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg
    32341 ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt
    32401 ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt
    32461 tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca
    32521 gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg
    32581 gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca
    32641 gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc
    32701 cggcgatggc gctcatcagg attggaccgc cacgataaaa tccggcgcga tacatcactt
    32761 gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa
    32821 ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc
    32881 cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt
    32941 tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc
    33001 ggtgcgttct acggactgcc cggcgcgata aagatcgctg cctaagcccg ccccaacaca
    33061 acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc
    33121 gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga
    33181 cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt
    33241 cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg
    33301 gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg
    33361 tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg
    33421 aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc
    33481 aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat
    33541 cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg
    33601 tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg
    33661 cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca
    33721 tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg
    33781 gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg
    33841 cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca
    33901 aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg
    33961 tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca
    34021 taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc
    34081 gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt
    34141 cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg
    34201 gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt
    34261 cttcacgcca gtcggtcgtg acttctgcta acactgcagc cggagatttt ccgttcaggc
    34321 gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg
    34381 cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt
    34441 taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc
    34501 ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc
    34561 ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct
    34621 aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc
    34681 cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc
    34741 aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc
    34801 caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag
    34861 cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc
    34921 cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg
    34981 gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga
    35041 gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt
    35101 tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat
    35161 cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgcgcgc
    35221 gtaatgtagt acaaacttat attgttgcgc tacaatttag atcaaaaaaa gaacaacaca
    35281 taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag
    35341 ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt
    35401 gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg
    35461 ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc
    35521 gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta
    35581 tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa
    35641 gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt
    35701 tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa
    35761 taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg
    35821 taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta
    35881 ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc
    35941 cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta cactggacat
    36001 cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg
    36061 gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag
    36121 caaacgccac gccatcttca aggttggatt ttgtgacaaa gttcgccact tctttcaccg
    36181 aaggaatagc gttatccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt
    36241 tttcctgatc gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg
    36301 atttcacacc cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac
    36361 ttttcagcac ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga
    36421 ggatggcggg ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct
    36481 ccgcttcgca gaacaccagc ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc
    36541 tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga
    36601 cttcgcgaga gagtttttcc aggaaacgat agtcgtcata gctgagagcg gtttgcgcca
    36661 cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc
    36721 ccggctgttc catatgcagc tctttcaggt agttgtgcac acctgcatac ggacgacccg
    36781 tcgttagcac gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg
    36841 gtgaaatggt gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga
    36901 tagccatgag ttcccaaggt agattagttc ctgacccatg ctaacgcgat tccgctcaaa
    36961 aatcagtaca acacccgagg gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc
    37021 ttagcggaag agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc
    37081 ttgtgctgac acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc
    37141 cagtaaactg gaagccgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc
    37201 atttgtgccg agcgccgact ccagttgctc gccgctaact gattgatttc tctgttgatt
    37261 actcaaccag gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac
    37321 ctgaatgcca ccctgctcct caacccaact taaaatcgcc tgatatttcc ccgcatcgcc
    37381 tttcagaaag gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt
    37441 acgtgttgac ccaaaaagta tagatctgcg ggatgataat tgcggattgc agaaataaaa
    37501 agggcggaga tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc
    37561 ttacccggcc tacaaaatca tgcaaaatca atatattgca ggagtctcgt aggactaata
    37621 agcgtagcgc atcaggcacg ctcgcatggt taacgccatt aaatatcaat attcgccgct
    37681 ttcagggcgt tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg
    37741 gtgaacaact ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt
    37801 tccgggtcca tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa
    37861 cgctggatgg agaggccacg acgggactct ttcaccagcc agtccagcgc ctgctcgaag
    37921 ctggctaccg gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca
    37981 cgcagtttct cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga
    38041 tccagcggat agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg
    38101 ttttgttcgg cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg
    38161 ttcagttcgc tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca
    38221 gcttccgtca acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc
    38281 tccatgcggt tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct
    38341 tcgccagcca atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata
    38401 gagatctggt actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc
    38461 ttcactttgt acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc
    38521 atctgacgat agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca
    38581 tcggtcatga tgatgatgct gtgataacgc agtttgtccg ggttgtactc gtcacgaccg
    38641 ataccacagc caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg
    38701 aagcgcgctt tctcgacgtt aaggatttta cccttcagcg gcagaatcgc ctggttcttg
    38761 cggttacgcc cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg
    38821 gaaagcgccg gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc agctaagtcg
    38881 agcgcacctt tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca
    38941 gcatcgataa ttttgccaac cacgattttc gcatcagttg ggttttccag caggtattcc
    39001 gccagcagtt cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg
    39061 tctttggtct gggaggagaa tttcgggtcc ggcactttta cggaaacgac cgcaatcagg
    39121 ccttcacgcg catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg
    39181 tccatgtagg cattcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg
    39241 ccgtcacgct gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc
    39301 cactgcagcg ccacttcaac gccaataccg tctttttcgg tggagaagta gaagatattt
    39361 gggtggatcg gcgttttgtt cttgttcaga tattcaacga acgctttgat gccgccttca
    39421 tagtggaagt ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag
    39481 ttgaggaacg acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca
    39541 ttggtgaagg tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg
    39601 ccggtaaccg ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta
    39661 ccctcgcgct gaataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca
    39721 acgccgtgca gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc
    39781 agaacggtca tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga
    39841 atgccgcgcc cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt
    39901 tctttacagt gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg
    39961 tggtgcagac cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc
    40021 gcatccagcc ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt
    40081 tctcgctcat ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt
    40141 cgaattttcg tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg
    40201 tgattgcgtc gcttttaagc ggctggcaag caacccgcga cgctcatcat caagctcaga
    40261 ggcaaaatca tctatcaggt agagacaccg ccgcccgctt tcacgagtga ggaactctcc
    40321 ttgcgccaga cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc
    40381 caccggcgca ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta
    40441 ggttagctgg cgatcgcgtt caaaattacg ttccagcacc tcagcgtact ctgtctcttt
    40501 ctcccagccg cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt
    40561 atcatccata tcggccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc
    40621 cgccagcggg atcagttctt tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg
    40681 cagcgccgca ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc
    40741 aggttcgttg tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt
    40801 gagtaaagta aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc
    40861 gactttatgc ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt tgtctttggt
    40921 taagccaatc gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc
    40981 ctgctcatgg cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag
    41041 cgtatagatg gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt
    41101 aaagccggga gataaagcga gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa
    41161 gcgggtgagg gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg
    41221 gcatcttcaa tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat
    41281 ttcagcgcgt tcagaacatc cagcacatag ctgacgttga agccgatttc catctccgca
    41341 ccgctatagg taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcagta
    41401 attttcagct ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga
    41461 atcgccgcgc gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc
    41521 gggttcttcg gcagaacgcg acggtaatcc gggaagcgac catccaccag tttcgaggtg
    41581 aaaataaagt cgccaacgtg ggcacgaatg ttattgctgc caatctgcac acgcagcaga
    41641 ttgtcgccgc cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc
    41701 gaatggcttg gcaaagattg accaattggc attgaacaga ccgccagacg gtggccgtcg
    41761 gttgccacgg tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag
    41821 cgaacatcct gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc
    41881 tgcggcaggg taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct
    41941 ggcagggtag acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc
    42001 agctgcacgg caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc
    42061 gccggaacgg tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt
    42121 tccatctcga gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga
    42181 ttaccgagaa tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt
    42241 tttaataaat gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat
    42301 taaatttgaa aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg
    42361 gcaggcatga agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag
    42421 actgtggtta gtcagctctt tcgctagcgc catcgccatc tggcgcggac gcgccaccga
    42481 gcgggatcga cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt
    42541 cttctgaata ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc
    42601 ctcacgcacg aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg
    42661 gttcagcgcc ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc
    42721 cacttcaccc ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacacg
    42781 ggtttccagc tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa
    42841 acgatcctca acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg
    42901 attaccttcc agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt
    42961 agcaaaaaac tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa
    43021 ctcttcgatc gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg
    43081 catataaacc actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag
    43141 caggtgagtt ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc
    43201 agggttatcc gccacctggc aagccgccgc gcgcgccagt tggttagatt taccttcaac
    43261 gaagttatca aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc
    43321 cgggacgtta tcccaacctg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc
    43381 cacctgtgca ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg
    43441 tttggtgccg acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag
    43501 tccattgata ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc
    43561 gtacagggcc agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa
    43621 ttctgtggct ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga
    43681 cacggcggac tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg
    43741 ttgacgtacg tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat
    43801 atgcccgatc aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa
    43861 gagatcttct gtttctcaca gattttccct atttatccac aggactttcc agaactcgct
    43921 aagtgtaaac gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa
    43981 tgaccataga caaaaattgg cttaatcgat ctaataaaga tccaggacga tccttgcgct
    44041 ttacccatca gcccgtataa tcctccaccc ggcgcgccat gctggtttcc actggtgtga
    44101 ggtcgtacat tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt
    44161 gactccggtg tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc
    44221 catcgtttca tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta
    44281 gaaatcgcca tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc
    44341 ttccgtgctc gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa
    44401 ggccgcgctc gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc
    44461 atttcccagg gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc
    44521 acaacgggct ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc
    44581 ccgtatcggt cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat
    44641 taaacgcctg acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt
    44701 cgtggtggtg gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt
    44761 ggaaaaatta tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg
    44821 gtctatcaac gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt
    44881 tcaagctacg gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg
    44941 gtgaaacgcg tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc
    45001 ggaccatttg ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg
    45061 ctttgctgtt cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac
    45121 ctcaggccca acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg
    45181 gcgtaccggc cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga
    45241 ccatcaacac ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc
    45301 tgaactctac ccagccgttc cagctgttgg aaacttcacc gcagtttatt tatcaggcac
    45361 agagcggtct gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt
    45421 ataacgttga aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga
    45481 tgacgtatac cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg
    45541 attacgctgt caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct
    45601 cctcgtttgg tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca
    45661 gcaacttcgc actgcacacc ttccgtggcg cggcgtactc cacgcctgac gagaagtatg
    45721 agaaatacaa gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg
    45781 gttgggtggc gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta
    45841 ccaacaactt ctataccgct aatctgggta acggcatcgt cgctatcggc tataaatctc
    45901 agccggtact ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc
    45961 cggaaatcca ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg
    46021 gttggttgtg gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg
    46081 tgggtaactg gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc
    46141 cgctgaccaa agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc
    46201 aggcaatgcg tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc
    46261 tgtacaaagc tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc
    46321 caatcttcct ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt
    46381 ttgcactgtg gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga
    46441 tgggcgtaac gatgttcttc attcagaaga tgtcgccgac cactgtgacc gacccgatgc
    46501 agcagaagat catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt
    46561 caggtctggt actgtactat atcgtcagca acctggtaac cattattcag cagcagctga
    46621 tttaccgtgg tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg
    46681 gtgagttttc gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg
    46741 ataagcaccg cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta
    46801 tcgtagccca ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggct
    46861 tcaaagcccg tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg
    46921 attatcttcc gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt
    46981 tccctggccc gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc
    47041 cggtgatcct cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc
    47101 gccctggtga gttttccgaa cgcgcgtttc ttaacgataa acttgactta gcccaggccg
    47161 aggcgattgc cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact
    47221 cgctgcaagg cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc
    47281 gcatttacgt cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg
    47341 gcaaaattga agcccagctt aatgacgtga tggccgatct cgatgcagtg cgtgctgaag
    47401 cacgtcaggg tagtttgttg cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg
    47461 ccggtaaatc gagcctgtta aacgcgctgg cgggccgtga agcggcaatc gtaaccgata
    47521 tcgccggaac cacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc
    47581 atatcatcga cactgccggg ctacgtgagg ccagtgacga agtggaacgt attggtatcg
    47641 agcgcgcgtg gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca
    47701 cgacagacgc cgttgatccg gcagagatct ggccggaatt tatcgcccgt ctgcccgcga
    47761 aactgccgat caccgtggta cgcaataaag ccgatatcac cggcgaaacg ctgggaatga
    47821 gtgaagtgaa cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg
    47881 tgctgcgtaa ccatctcaaa cagagcatgg gctttgacac caatatggaa ggcggcttcc
    47941 tggcgcgccg tcgccactta caggcgctgg aacaggcagc ggagcatttg cagcaaggta
    48001 aagcgcaact gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgcctggcac
    48061 agcagaactt aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt
    48121 tctccagctt ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat
    48181 gcggcttcgc ttcattgtta gcactcctgt tatgcctcaa ccctttttta aacattaaaa
    48241 ttcttacgta atttataatc tttaaaaaaa gcatttaata ctgctccccg aacgattgtg
    48301 attcgattca catttaaaca atttcagaat agacaaaaat t
//
LOCUS       DAJTMX010000013        48327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTMX010000013 DAJTMX010000000
VERSION     DAJTMX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.013"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..653)
                     /gene="pcnB"
                     /locus_tag="OO145_000786"
     CDS             complement(<1..653)
                     /gene="pcnB"
                     /locus_tag="OO145_000786"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5466000.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAA"
     gene            complement(713..1639)
                     /gene="gluQRS"
                     /locus_tag="OO145_000787"
     CDS             complement(713..1639)
                     /gene="gluQRS"
                     /locus_tag="OO145_000787"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5466001.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(1676..2131)
                     /gene="dksA"
                     /locus_tag="OO145_000788"
     CDS             complement(1676..2131)
                     /gene="dksA"
                     /locus_tag="OO145_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5466002.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(2309..3013)
                     /gene="sfsA"
                     /locus_tag="OO145_000789"
     CDS             complement(2309..3013)
                     /gene="sfsA"
                     /locus_tag="OO145_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5466003.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(3028..3558)
                     /gene="thpR"
                     /locus_tag="OO145_000790"
     CDS             complement(3028..3558)
                     /gene="thpR"
                     /locus_tag="OO145_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5466004.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            3632..6061
                     /gene="hrpB"
                     /locus_tag="OO145_000791"
     CDS             3632..6061
                     /gene="hrpB"
                     /locus_tag="OO145_000791"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5466005.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            6257..8791
                     /gene="mrcB"
                     /locus_tag="OO145_000792"
     CDS             6257..8791
                     /gene="mrcB"
                     /locus_tag="OO145_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5466006.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            9011..11254
                     /gene="fhuA"
                     /locus_tag="OO145_000793"
     CDS             9011..11254
                     /gene="fhuA"
                     /locus_tag="OO145_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5466007.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            11305..12102
                     /gene="fhuC"
                     /locus_tag="OO145_000794"
     CDS             11305..12102
                     /gene="fhuC"
                     /locus_tag="OO145_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5466008.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            12102..12992
                     /gene="fhuD"
                     /locus_tag="OO145_000795"
     CDS             12102..12992
                     /gene="fhuD"
                     /locus_tag="OO145_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5466009.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            12989..14971
                     /gene="fhuB"
                     /locus_tag="OO145_000796"
     CDS             12989..14971
                     /gene="fhuB"
                     /locus_tag="OO145_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5466010.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(15006..16286)
                     /gene="hemL"
                     /locus_tag="OO145_000797"
     CDS             complement(15006..16286)
                     /gene="hemL"
                     /locus_tag="OO145_000797"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5466011.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            16511..17932
                     /gene="clcA"
                     /locus_tag="OO145_000798"
     CDS             16511..17932
                     /gene="clcA"
                     /locus_tag="OO145_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5466012.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            17956..18021
                     /locus_tag="OO145_000799"
     CDS             17956..18021
                     /locus_tag="OO145_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466013.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            18014..18358
                     /gene="erpA"
                     /locus_tag="OO145_000800"
     CDS             18014..18358
                     /gene="erpA"
                     /locus_tag="OO145_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5466014.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(18405..19028)
                     /locus_tag="OO145_000801"
     CDS             complement(18405..19028)
                     /locus_tag="OO145_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5466015.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(19066..19866)
                     /gene="btuF"
                     /locus_tag="OO145_000802"
     CDS             complement(19066..19866)
                     /gene="btuF"
                     /locus_tag="OO145_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5466016.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(19859..20557)
                     /gene="mtnN"
                     /locus_tag="OO145_000803"
     CDS             complement(19859..20557)
                     /gene="mtnN"
                     /locus_tag="OO145_000803"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5466017.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            20641..22158
                     /gene="dgt"
                     /locus_tag="OO145_000804"
     CDS             20641..22158
                     /gene="dgt"
                     /locus_tag="OO145_000804"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5466018.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            22288..23712
                     /gene="degP"
                     /locus_tag="OO145_000805"
     CDS             22288..23712
                     /gene="degP"
                     /locus_tag="OO145_000805"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5466019.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            23867..25023
                     /gene="cdaR"
                     /locus_tag="OO145_000806"
                     /pseudo
     CDS             23867..25023
                     /gene="cdaR"
                     /locus_tag="OO145_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(25112..25498)
                     /locus_tag="OO145_000807"
     CDS             complement(25112..25498)
                     /locus_tag="OO145_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5466020.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(25812..26636)
                     /gene="dapD"
                     /locus_tag="OO145_000808"
     CDS             complement(25812..26636)
                     /gene="dapD"
                     /locus_tag="OO145_000808"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5466021.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(26667..29339)
                     /gene="glnD"
                     /locus_tag="OO145_000809"
     CDS             complement(26667..29339)
                     /gene="glnD"
                     /locus_tag="OO145_000809"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5466022.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNILQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(29401..30195)
                     /gene="map"
                     /locus_tag="OO145_000810"
     CDS             complement(29401..30195)
                     /gene="map"
                     /locus_tag="OO145_000810"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5466023.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            30563..31288
                     /gene="rpsB"
                     /locus_tag="OO145_000811"
     CDS             30563..31288
                     /gene="rpsB"
                     /locus_tag="OO145_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5466024.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            31546..32397
                     /gene="tsf"
                     /locus_tag="OO145_000812"
     CDS             31546..32397
                     /gene="tsf"
                     /locus_tag="OO145_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5466025.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            32544..33269
                     /gene="pyrH"
                     /locus_tag="OO145_000813"
     CDS             32544..33269
                     /gene="pyrH"
                     /locus_tag="OO145_000813"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5466026.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            33561..34118
                     /gene="frr"
                     /locus_tag="OO145_000814"
     CDS             33561..34118
                     /gene="frr"
                     /locus_tag="OO145_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5466027.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            34210..35406
                     /gene="ispC"
                     /locus_tag="OO145_000815"
     CDS             34210..35406
                     /gene="ispC"
                     /locus_tag="OO145_000815"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5466028.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            35595..36353
                     /gene="ispU"
                     /locus_tag="OO145_000816"
                     /gene_synonym="uppS"
     CDS             35595..36353
                     /gene="ispU"
                     /locus_tag="OO145_000816"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5466029.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            36366..37223
                     /gene="cdsA"
                     /locus_tag="OO145_000817"
     CDS             36366..37223
                     /gene="cdsA"
                     /locus_tag="OO145_000817"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5466030.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            37235..38587
                     /gene="rseP"
                     /locus_tag="OO145_000818"
     CDS             37235..38587
                     /gene="rseP"
                     /locus_tag="OO145_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5466031.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            38617..41049
                     /gene="bamA"
                     /locus_tag="OO145_000819"
     CDS             38617..41049
                     /gene="bamA"
                     /locus_tag="OO145_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5466032.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            41171..41656
                     /gene="skp"
                     /locus_tag="OO145_000820"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             41171..41656
                     /gene="skp"
                     /locus_tag="OO145_000820"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5466033.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            41660..42685
                     /gene="lpxD"
                     /locus_tag="OO145_000821"
     CDS             41660..42685
                     /gene="lpxD"
                     /locus_tag="OO145_000821"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5466034.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            42790..43245
                     /gene="fabZ"
                     /locus_tag="OO145_000822"
     CDS             42790..43245
                     /gene="fabZ"
                     /locus_tag="OO145_000822"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5466035.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            43249..44037
                     /gene="lpxA"
                     /locus_tag="OO145_000823"
     CDS             43249..44037
                     /gene="lpxA"
                     /locus_tag="OO145_000823"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5466036.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44037..45185
                     /gene="lpxB"
                     /locus_tag="OO145_000824"
     CDS             44037..45185
                     /gene="lpxB"
                     /locus_tag="OO145_000824"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5466037.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            45182..45778
                     /gene="rnhB"
                     /locus_tag="OO145_000825"
     CDS             45182..45778
                     /gene="rnhB"
                     /locus_tag="OO145_000825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5466038.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            45815..>48327
                     /gene="dnaE"
                     /locus_tag="OO145_000826"
     CDS             45815..>48327
                     /gene="dnaE"
                     /locus_tag="OO145_000826"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5466039.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGA"
BASE COUNT        11309 a        11920 c        13460 g        11638 t
ORIGIN      
        1 ttggcggcaa aacgtaccgc gcgcagcata cgtaccggat cttcacggta gcgcgtttcc
       61 gggttaccaa tcagacggat aacgccgtcc ttcagatcct tcataccacc aacgtaatca
      121 cggacggtaa aatccgctac gctgtaatac aggctgttga tagtgaaatc gcggcgctgg
      181 gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt
      241 tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt cgcaacttcg
      301 ataatctccg ggccaaacat tacatgagcc agacggaaac ggcgacccac caggcggcag
      361 ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc
      421 ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc
      481 gctttgttga gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata
      541 gcatgctgct cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg
      601 ctttcctcgc ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac
      661 ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg
      721 acgcatttga gaatgttgaa tttacaattg ccgactcggg cacggcggtc agcatccagt
      781 ttttgacggc tgactgaagg atttgctcga cgctgaaatc ctgccagtgt gcttctgcct
      841 gctgccccag aaattgaagt gccgcgatta gtaccgggcg tggatcgcct ttcggcaacg
      901 caggcgcatg attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat
      961 gaatgtaatc tggcactttc cagccaaaaa gctggtacag cgggatttgc cttactgttg
     1021 gttcaatcag atcagcccca cgcactattt ctgtaacgcc ctggaaatga tcatcaacca
     1081 caacagccag gttgtaggcg aacaacccat cacggcgatg aatgataaaa tcttcccgtg
     1141 ccagtttttc gtcggcgtga ataatgcctc gcagcaggtc agtaaattgc gtgaccggat
     1201 gctgctggcg gatacgcact gcggcgttgt ctggtccatg atgcaacacc cggcaatgac
     1261 cgtcgtaaat accgccaatg ctttgaatac gcgcacgcgt acaggtgcag taataactta
     1321 gtccttgttc atgtaaccag gcgagtgctt cacgataggc gtcgtgacgt tgcgattgcc
     1381 agagaacatc gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg
     1441 cggcaccggg aacttcacga ggcgggtcga tatcttctat gcgtaccagc cagcgacctt
     1501 gccgggcgcg agcctgcaaa tagctgccga gcgcggcgat cagagagcca aaatgaagct
     1561 cgccggaagg agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca
     1621 aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc
     1681 cagccatctg tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg
     1741 tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc gcaggattcg cagtagccga
     1801 aatcttcgtc ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc
     1861 gatcgcggtt acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt
     1921 ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga
     1981 gttgattacg ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat
     2041 actcttcgcc cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg
     2101 acgatgtttt acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat
     2161 ccccatgttc gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa
     2221 ttatctaaac gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag
     2281 aatgtaaatt aaccagttac ttactttact acaatgtaac cggcagtgat tttttaagag
     2341 ccatgccttc agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag
     2401 cttctgacaa tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa
     2461 tggctgaatg cagcacggcg aaaaaaataa ccgcacgctg gccttcagcc gctacgctca
     2521 tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt
     2581 cgttctccgc taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct
     2641 gcaacataaa gtcaatacgg ctgcgttcgg agccgtattt tacttcgctt ttcagcgagc
     2701 tatagcctga cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag
     2761 cccaaagcgt attgacgcaa ataaatgcgc cgctctggct ttgagttaat tcccaggtgt
     2821 gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccaggcgttg
     2881 cacaaccggt catcgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg
     2941 tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg
     3001 gagaaaattc cattgcgaca atccttgtta ttgcgttagc gcccagcgtt ttagcggcgt
     3061 gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata
     3121 agaccagtta aaacccggcg gcgggaatgt caccgcctcg ctggcgtcgc gcaataaggt
     3181 aatatgtgga tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg
     3241 gagcatattc gccagctgga ttaagccgcg tggaggctga cgcatcccta accacaccac
     3301 acgcgaacgc agccattgtc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat
     3361 ccgtccggct aaaagagaaa gcgccttctc tttctctgcg ctcacttcgc ctaaaaatgc
     3421 cagagtcagg tgcaaattat cggcggcgac cggacgtccc gcctcaggtg ggaagtgtgt
     3481 ggcgcgccaa tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag
     3541 acgttgcggt tcagacatgt gaggcactcg gttatgaatt accgcgatgc tacaatgtgg
     3601 cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtctt
     3661 acctgaatta cttaccgctc tcgattgtgc gccgcaggta ttattaagtg cgccgaccgg
     3721 ggccgggaaa tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa
     3781 aattatcctg ctggagccgc gtcgtctggc ggcgcgtaac gtcgcgcaac ggctggcgga
     3841 gctgcttaac gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt
     3901 cgggctgaat acccgcctgg aagtggttac cgaaggcgtg ctgacgcgga tgatccagcg
     3961 tgacccggaa ctgagcggtg ttggattggt gatccttgat gagtttcatg agcgcagttt
     4021 gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct
     4081 taaactgctg attatgtcgg ctacgctgga caatgaccgc ttgcagcaaa tgctgccgga
     4141 agcgcccgtc gtcatctcag aagggcgctc gtttccggtt gaacgccgtt atttaccgct
     4201 gcccgcgcat cagcgttttg acgaagccgt tgcggtagcc accgccgaaa tggtgcgtca
     4261 ggaaagtgga tcattactgt tatttttacc tggcgtcgga gaaattcagc gcgtgcagga
     4321 acaactggct tcgcgcatcg gcagtgatgt ggtgctctgc ccgctgtatg gcgtgttgtc
     4381 actgaacgat cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct
     4441 ggcgaccaat attgctgaaa ccagtttaac catcgaaggt attcgtctgg tggtggattg
     4501 tgcccaggag cgtgtggcgc gctttgatcc gcgcacgggg cttacgcggt tgattactca
     4561 acgcgttagt caggcatcca tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg
     4621 tatcagcctg catttaatcg ccaaagaaca agcagaacgc gccgcggcgc aaagtgagcc
     4681 ggagatctta caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag
     4741 cgatccggcg cagatgagct ggctggatca accgccaacg gtgaatctac tggccgcgaa
     4801 acgcctgtta caaatgctgg gggcgctgga gggtgaacgg cttagtgcgc aagggcaaaa
     4861 aatggcagcg ctgggtaacg atccgcgttt agcggcaatg ctggttaacg caaagagcga
     4921 tgacgaagct gctaccgcgg caaaaattgc cgccattctc gaagagccgc cacggatggg
     4981 caatagtgac ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca
     5041 gcaactgtta aaacgcttaa acgtacgcgg cggtgaggca gacagttcgc ttatcgcgcc
     5101 gctacttgcc ggggcgtttg ccgatcgcat tgctcatcgc cgtgggcaag atggacgcta
     5161 tcaactggca aacagcatgg gagcgatgct cgatgccgac gacgcgctaa gccgccacga
     5221 atggttgatc gcaccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact
     5281 ggcgctaccg gtcgatattg atgaattagt acaacgctgt ccgcagctgg tgcagcaatc
     5341 tgacaccgtg gaatgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctacaaat
     5401 cggtcagttg acggtgaaag tgcagccgct ggcgaaaccg tcagaagacg agttgcatca
     5461 ggcgatgctt aatggcatcc gtgataaagg tttaagcgtg ctcaactgga cggcggaagc
     5521 ggaacagcta cgcttgcgtt tgttatgcgc cgcaaagtgg ttgccggaat atgactggcc
     5581 agcggttgat gatgaaagtt tgttggcgac gctggaaacg tggctgctgc cacatatgac
     5641 cggcgtacat tcactacgcg gcctgaaatc actcgacatt tatcaggcac tacgcggatt
     5701 acttgattgg ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc
     5761 gacgggaagc cggatcgcca ttcgttatca tgaagataac ccgcccgcgc tggcggtgag
     5821 aatgcaggag atgtttggtg aggccactaa tccgacgatc gcccaggggc gcgtgccgct
     5881 ggtgctgaag ttgctttccc ctgcccaaag gccgctgcaa atcacgcgtg atttgggggc
     5941 cttctggaaa ggggcgtatc gtgaggtgca aaaggagatg aaagggcgtt atcccaaaca
     6001 cgtctggccg gacgacccgg caaacacggc accgacgcga cggacgaaaa agtattcgta
     6061 aggttggttt tctccctctc cctgtgggag agggccgggg tgagggcatc agcgcgcacg
     6121 ttcaccctca ccctaaccct ctccctcaag ggagagggga ccgatcgagc gcaaattttg
     6181 agagatatct tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt gcgcctgaat
     6241 attgcggaga aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaacc
     6301 gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg atgattacga
     6361 cgattatgat gactatgagg atgaagaacc gatgccgcgc aaaggtaagg gcaaaggcaa
     6421 agggcgtaag cctcgtggca aacgcggctg gctatggcta ctgctaaaac tggctatcgt
     6481 ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat caaaaaattc gtagccgtat
     6541 tgatggcaag gtctggcaac tgcccgcggc agtttatggc cgaatggtca accttgaacc
     6601 agatatgacc atcagcaaga acgaaatggt gaagctgctg gaggcaacac agtatcgtca
     6661 ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag gccaacagca ttgagatgat
     6721 ccgccgtccg tttgatttcc cggacagtaa agaagggcag gtgcgtgcgc gtctgacctt
     6781 tgatggtgat catctggcga ccatcgtcaa tatggagaac aaccgtcagt tcggtttctt
     6841 ccgtcttgat ccgcgtctga tcaccatgat ctcctcgcca aacggtgagc agcgtttgtt
     6901 tgtgccgcgc agtggtttcc ctgatttgct ggtggatact ttgctggcga cagaagaccg
     6961 tcatttttac gagcatgatg gaatcagtct ctactccatt gggcgtgcgg tgctggcaaa
     7021 cctgaccgca ggtcgcacgg tgcagggtgc gagtacgctg acgcaacagc tggtgaaaaa
     7081 cctgttcctc tccagcgagc gttcttactg gcgtaaagcg aacgaagctt acatggcgct
     7141 gatcatggac gcgcgttaca gcaaagaccg tattcttgag ctgtatatga acgaggtgta
     7201 tctcggtcag agcggcgaca acgaaatccg cggcttcccg ctggcaagct tgtattactt
     7261 tggtcgcccg gtagaagagc taagcctcga ccagcaggcg ctgttagtcg gtatggtgaa
     7321 aggggcgtcc atctacaacc cgtggcgtaa cccgaaactg gcgctggagc gacgtaatct
     7381 ggtgctgcgt ctgctgcaac agcaacagat tattgatcaa gaactctatg acatgttgag
     7441 tgcccgtccg ctgggggttc agccgcgtgg tggggtgatc tctcctcagc cagcctttat
     7501 gcagctggtg cgtcaggagt tgcaggcaaa actgggcgat aaggtaaaag atctctccgg
     7561 cgtgaagatc ttcactacct ttgactcggt ggcccaggac gcggcagaaa aagccgccgt
     7621 ggaaggcatt ccggcactga agaaacagcg taagttgagc gatcttgaaa ctgcgattgt
     7681 ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtc ggaggttctg agccgcagtt
     7741 tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg caaaaccagc
     7801 gacttatctg acggctttaa gccagccgaa aatctatcgc ctgaacacgt ggattgccga
     7861 tgcgccaatc gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga acgatgaccg
     7921 tcgttatagc gaaagcggca gagtgatgct ggtggatgcg ttgacacgtt cgatgaacgt
     7981 gccgacggta aatctgggga tggcgctggg gctgcctgcg gttacggaga cctggatcaa
     8041 actgggcgta ccgaaagatc agttgcatcc ggttccggca atgctgctgg gggcgttgaa
     8101 cttaacgcca atcgaagtgg cgcaggcatt ccagaccatc gccagcggtg gtaaccgtgc
     8161 accgctttct gcactgcgtt cggtaatcgc ggaagatggt aaggtgctgt atcagagctt
     8221 cccgcaggca gaacgcgctg ttccggcgca ggcggcgtat ctgacactat ggaccatgca
     8281 gcaggtggta caacgtggta cgggtcgtca gcttggggcg aaatacccga acctgcatct
     8341 ggcagggaaa acagggacta ccaacaataa cgtagatacc tggtttgcag gcattgacgg
     8401 cagcacggtg accatcacct gggtaggccg tgataacaac cagccgacca aactgtatgg
     8461 agccagcggg gcaatgtcga tttatcagcg ttatctggct aaccagacgc caacgccgct
     8521 gaatcttgtt ccgccagaag atattgcaga tatgggcgtg gactacgacg gcaactttgt
     8581 ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat cgctgtgcca
     8641 gcagagcgag atgcagcagc agccgtcagg caatccgttt gatcagtctt ctcagccgca
     8701 gcaacagccg caacagcaac ctgctcagca agagcagaaa gacagcgacg gtgtagccgg
     8761 ttggatcaag gatatgtttg gtagtaatta acatctaagc gtgaaatacc ggatggcgag
     8821 ttgccatccg gtaaaataac atcccatcta agatattaac cctttctttt catctggttg
     8881 tttattaacc cttcaggaac gctcagattg cgtaccgctt gcgaacccgc cagcgtttcg
     8941 aatattatct tatctttata ataatcattc tcgtttacgt tattattcac tttacatcag
     9001 agatatacca atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc
     9061 agttgtagta gccacagcgg ttagcggcat gtctgtttat gcacaggcag cggttgaacc
     9121 gaaagaagac actatcaccg ttaccgctgc acctgcgccg caagaaagcg catgggggcc
     9181 tgctgcaact attgcggcgc ggcagtcagc taccggcact aaaaccgata cgccgattca
     9241 aaaagtgcca cagtctattt ctgttgtgac cgccgaagag atggcgctgc atcagccgaa
     9301 gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc
     9361 caacacctat gaccacctga tcattcgcgg ttttgcggca gaaggccaaa gccaaaataa
     9421 ctatctgaat ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgacccgta
     9481 tatgctggaa cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag
     9541 tcctggcggt ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc tgaaagaagt
     9601 tcagtttaaa gccggtactg acagcctgtt ccagactggt tttgacttta gcgatgcgtt
     9661 ggatgatgac ggtgtttact cttatcgcct gaccggtctt gcgcgttctg ccaatgccca
     9721 gcagaaaggg tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga
     9781 tgataaaacc aatttcacct tcctttctta cttccagaac gagccggaaa ccggttatta
     9841 cggctggttg ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac
     9901 agactttaat gaaggggcga agaacaacac ctattctcgt aatgagaaga tggtcggcta
     9961 cagcttcgat cacgaattta acgacacctt tactgtgcgt cagaacctgc gctttgctga
    10021 aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc
    10081 ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa ggccattatc tggcacgtaa
    10141 atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa
    10201 gtttgccact ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg
    10261 taatgacatc aacgcctggt ttggttacga cgactctgtg ccactgttca atctgtacaa
    10321 tccggtgaat cccgatttcg acttcaatgc caaagatccg gcaaactccg gcccttaccg
    10381 cattctgaat aaacagaaac aaacgggcgt ttatgttcag gatcaggcgc agtgggataa
    10441 agtgctggtc accctaggcg gtcgttatga ctgggcagat caagaatctc ttaaccgcgt
    10501 tgctgggacg accgataaac gtgatgacaa acagtttacc tggcgtggtg gtgttaacta
    10561 cctgtttgat aatggtgtaa caccttactt cagctatagc gaatcgtttg aaccttcttc
    10621 gcaagttggg aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg
    10681 cgtgaaatat gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac
    10741 taaaaccaac aacctgatgg cggaccctga gggttccttc ttctcggttg aaggtggcga
    10801 gatccgcgca cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt
    10861 agtcggttct tatacttaca ccgatgcgga atacaccacc gatactacct ataaaggcaa
    10921 tacgcctgca caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga
    10981 cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg
    11041 tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta
    11101 tgatctggcg cgagtcggca tggcgggctc caacgtggcg ctgcatgtta acaacctgtt
    11161 cgatcgtgaa tacgtcgcca gctgctttaa cacttatggc tgcttctggg gcgcagaacg
    11221 tcaggtcgtt gcaaccgcaa cctttcgttt ctaatttctc ttttggggca cggatttccg
    11281 tgcccatttc acaagttggc tgttatgcag gaatacacga atcattccga taccactttt
    11341 gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta
    11401 acctttcctg ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact
    11461 ctgctcaaaa tgcttggtcg tcatcagcct ccgtcagaag gggagattct tcttgatgcc
    11521 cagccgctgg aaagctggag cagcaaagcg tttgcccgca aagtggctta tttgccgcag
    11581 cagcttcctc cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg
    11641 tggcatggcg cgctggggcg ctttggtgcg gcagatcgcg aaaaggtcga ggaagctatc
    11701 tcgctggttg gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa
    11761 cgtcagcggg cgtggatcgc catgctggtg gcgcaggata gccgttgtct gttgctcgac
    11821 gaaccgactt cagcgctgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt
    11881 ttaagtcagg aacgtggcct gacggtcatt gccgtgttgc acgatatcaa tatggcggca
    11941 cgctactgtg attatctggt cgccctgcgc agtggtgaaa tgattgctca gggaacgcct
    12001 gcggaaatta tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattttg
    12061 ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta
    12121 tttcgcgccg tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata
    12181 ccgcccacgc ggcggctatt gaccccaatc gtattgtggc gctggagtgg ttgccggtgg
    12241 aattactgct ggcgctcggc atcgtgcctt acggcgtggc ggataccatc aactatcgcc
    12301 tgtgggtcag cgaaccacca ttgccggact cagtgattga cgtcggtttg cgtacagaac
    12361 ctaaccttga actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg gcaggatatg
    12421 gcccttcatc agaaatgctg gcacgtattg cgccgggtcg cggatttaac ttcagtgacg
    12481 gcaaacatcc gttggcgatg gcgcgtaaat cgctgacgga aatggcagat ttacttaacc
    12541 tgcaaagcgc agcggaaacg catttagcgc aatacgaaga ctttatccgc agcatgaaac
    12601 cccgctttgt gaagcgtggt gcacgcccgt tactgctgac gacgcttatc gatccgcgcc
    12661 atatgctggt cttcggtcca aacagcttgt tccaggaaat tctcgatgag tacggcatcc
    12721 caaatgcctg gcaaggggaa accaacttct ggggcagtac cgccgtcagt atcgatcgtc
    12781 tggcagcgta taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg
    12841 atgcgctaat ggcaaccccg ctgtggcagg ccatgccgtt tgtccgtaca gggcgctttc
    12901 agcgcgtacc cgcagtctgg ttttatggtg caacgctttc ggcaatgcac tttgtgcgtg
    12961 ttctggataa cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt tcccggcgtt
    13021 attgctggcg ctgttagtga ttgtcgctac ggcgctcacc tggatgaact tctcacaggc
    13081 gctgccgcgc agtcagtggg cgcaggccgc ctggtcgccg gatattgacg tcatcgagca
    13141 gatgattttt cactacagct tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg
    13201 cctggggctg gtgggcgtgc tgtttcagca agtactgcgt aacccgctgg cggaaccgac
    13261 gacgttgggc gttgcaacag gcgcgcaact ggggattacc gtcaccacgc tctgggcgat
    13321 ccctagcgcg atggcgagcc agtttgctgc gctggcaggg gcttgcgttg ttggcttaat
    13381 tgtctttggc gtcgcgtggg ggaaacggct gtcgccggta acgctgattt tcgcggggtt
    13441 ggtagtgagc ctttattgcg gcgcaatcaa tcagttactg gttatcttcc atcatgacca
    13501 gttgcaaagc atgtttctgt ggagcactgg aacgctgacg caaaccgact ggggcggtgt
    13561 tgagcgttta tggccgcagt tgctgggcgg tgtgatgctg acgttgctgc tacttcgtcc
    13621 gttaaccctg atggggcttg atgatggcgt ggcgcgcaat ctcgggctgg ccttgtcgct
    13681 tgcgcgtctg gcggcgctgt cgctggcgat tgtcatcagt gcgctgctgg tgaacgctgt
    13741 ggggattatc ggctttatcg ggttgttcgc gccgctgctg gcaaaaatgc tgggggcgcg
    13801 gcgtctgctg ccacgactga tgctggcgtc gttgattggt gcgctgatcc tctggctttc
    13861 cgatcaaatc atcctctggc tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac
    13921 tgcgttgatc ggtgcgccgc tgctactgtg gctgctgccg cgtttacgca gcattagcgc
    13981 gccggatatg aaggttaacg atcgtgtcgc gactgaacgc caacatgtgc tggcgtttgc
    14041 cctcgcgggc ggcgtgctgt tgttgatggc tgtggtggtg gcgctgtcgt ttggtcgtga
    14101 tgcgcacggc tggacgtggg cgagcggggc gttgctcgag gatttaatgc cctggcgctg
    14161 gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg gcggtagcgg gctgtattat
    14221 tcagcgactg accggcaacc cgatggcaag cccggaagtg ctggggatta gctccggcgc
    14281 ggcgtttggt gtggtgttaa tgctgtttct ggtaccgggt aatgcctttg gctggttgtt
    14341 acccgcaggc agtctcgggg cggcggtgac gctgttgatc attatgatcg ccgccggacg
    14401 cggtggattt tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcgttcac
    14461 catgcttttg atgatgttgc aggcaagtgg tgacccgcga atggcgcaag tgctgacctg
    14521 gatttccggt tcgacctaca acgcgaccga tgctcaggtc tggcgcaccg gaattgtgat
    14581 ggtgattttg ctggcgatta ctccgctgtg ccgccgctgg ctgaccattt taccgctggg
    14641 tggtgatacc gcgcgggcgg tagggatggc gctgacgccg acgcgaattg cgctgctgct
    14701 gttagcggct tgcctgacgg cgaccgcgac gatgactatt ggaccgttga gttttgttgg
    14761 tttaatggca ccgcatattg cgcggatgat gggctttcga cggacgatgc cacacatcgt
    14821 aatttcggcg ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt
    14881 gctgtttcca ttccagatcc cggcggggct gctgtcgacc tttatcggtg cgccatattt
    14941 tatctatttg ctgagaaagc agagccgtta atagaatgcc tcgcccggtg ggcgaggcaa
    15001 aattatcaca acttcgcgaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc
    15061 atgctgtgcg ccacagacat aaagcctgct tcaaacgctg acggtgccag gtaaacacct
    15121 tcatccagca tcatatggaa gaaacgctta aaacgctcga cgtcacaggc catcacatcc
    15181 tgatagcacg tcacggactc ggcgtcggta aagaagatac cgaacatacc accgacgtgg
    15241 ttaacgacca gcggaattcc cgcttcttct gccgcttcca gcagaccttc tgccagacgt
    15301 gttgtcagct catccagcgt ttcgtgaatg cccggctgcg cgacttcatt cagacaggcg
    15361 aaacccgctg ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggacct
    15421 gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca
    15481 ccgccgatga tttttccgag gcaggttaaa tctggcacta cggcgaaata atcctgtgcg
    15541 ccagctagcg ctacgcggaa accggtcatc acttcatcga tgatcagcaa cgcgccaaat
    15601 tcgtcgcaca gcgcacgcag acctggcagg aactctggca gcggcggaac acagttcata
    15661 ttgcctgcca ccggctcgac gataatacag gcaatctctt gcgggtattg ctcaaacgcg
    15721 gcgcgtacag aggccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc
    15781 ggaacgcccg gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc
    15841 aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa tgattttgtc gcgaccggta
    15901 aaaccacggg ccaggcggat ggcgctcatc gtcgcctcgg tgccggagtt caccatgcgc
    15961 accatatcca tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg
    16021 gtcggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggatcgcc
    16081 ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg
    16141 ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc
    16201 acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg
    16261 ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc
    16321 tgctattcca tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact
    16381 ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc
    16441 aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag
    16501 tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg
    16561 acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt
    16621 tatggcggca gtcgtcggca cgcttgtcgg gctggctgcg gttgcttttg acaaaggtgt
    16681 cgcctggttg cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct
    16741 actgttaacc gtcgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt
    16801 gcgcaaatac gcgcccgaag cagggggatc ggggatcccg gaaattgaag gggcgctgga
    16861 agatcaacgt cctgttcgct ggtggcgtgt attgccggtg aaattctttg gcgggctggg
    16921 gacactcggc ggaggcatgg ggctagggcg cgaaggacca accgtgcaga tcggcggcaa
    16981 tatcagtcgc atggtgcttg atattttccg cctgaaaggt gatgaagctc gccatacgct
    17041 gctggcaacc ggtgctgctg cggggctggc tgcggccttt aacgcgccgc tggcgggtat
    17101 tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc
    17161 ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc
    17221 gttgattgac gtcggtaaac tttctgatgc gccgcttaat acgctgtggc tttatctgat
    17281 cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca
    17341 ggatttgctg caccgtgtgc acggcggcaa tattaccaaa tgggtactga tggggggggc
    17401 gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt
    17461 taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt
    17521 cgtcgctcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt
    17581 tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga
    17641 gctgtttccg caatatcacc tggaggcggg gacgtttgct attgccggaa tgggggcatt
    17701 actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga
    17761 taactaccag ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca
    17821 atttaccggc gggaaacctc tatactcggc gattcttgcg cgcacgctgg caaaacagga
    17881 agctgagcaa ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata
    17941 ccagggtatt agataatggc gattattatt gggttagaat ttgcccgatt gccgatgtcg
    18001 tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc
    18061 caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta
    18121 tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa
    18181 cgaaggcgat atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct
    18241 gcaatatctg gtcggcggtt ccgttgacta caccgaagga ctggaaggtt ctcgtttcat
    18301 cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc
    18361 tgttgttcat gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca
    18421 tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc
    18481 gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc
    18541 gtagcgtgga caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc
    18601 atggggattt cgcggaccag tacatcacga atgatcccgc cgccaacgcc agtaatgacg
    18661 cccatacaaa ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgctaatg
    18721 ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt
    18781 cttggctggc gcaccagcac gatggtcagc atgctggtga ccatggcaac gaccagatcg
    18841 gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgttccg
    18901 tcgcctactg cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt
    18961 ccggctaaca aaacgccgta gatggcaaat accgctgtgc cgactatatc cagccaatag
    19021 acgagcattg ttcaatcccc actgagcacc tgaaaaggtc aggcactaat ctacctgtga
    19081 aagcgcatta cagagctgtt gtgctgcgag gataatacgt gggcttgcac gttcaaacca
    19141 gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt
    19201 aggaatttgg tccggtccgc ctgtaatgac aatagcctgt ggcgagcgtg ctaacacctg
    19261 ttcgcggcta acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca
    19321 aacttcgagt acctggttct gaatcgactc ttttccactg gtaaatggcg gattaatgcc
    19381 gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcacgta
    19441 ctgatccagc agggattgcg cggcttgttc ggccttgtct ggttgcggac tccagggggc
    19501 cagttgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt
    19561 tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat
    19621 caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac
    19681 ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt
    19741 gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc
    19801 ggcgttgagc cacagtggcg cgagaaaaga cagggcgacc agcgccctga acagtgattt
    19861 agccatgtgc cagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa
    19921 cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc
    19981 gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca
    20041 tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt
    20101 tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa
    20161 tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg
    20221 gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt
    20281 ccgagacaac gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt
    20341 taataatcac atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga
    20401 ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga
    20461 tttcgcaacc gccgagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa
    20521 cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga
    20581 tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac
    20641 atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag
    20701 ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc
    20761 aactctccgg caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat
    20821 gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg acgctacatc
    20881 gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat
    20941 gaactgaccg gcccctttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc
    21001 aatccgccgt ttggtcattt tggcgaagca gcgataaatg actggtttcg ccaacgtttg
    21061 cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggcacta
    21121 cgtttacggg acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta
    21181 tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat
    21241 ctcacttggg cacaggttgg cggtatttta aaatataccc gtccggcgtg gtggcgtggc
    21301 gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa
    21361 gcctatattg cccgcttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta
    21421 acgtggatta tggaagctgc cgacgacatc tcctattgtg tggcagacct tgaagatgcg
    21481 gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga agcgtggggc
    21541 cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc
    21601 tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc
    21661 ctaaataaac tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc
    21721 gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag
    21781 ctatataaaa atgtcgctgt aaaacatgtg tttagccatc cggatgtcga gcagcttgaa
    21841 ttgcagggct atcgggtcat tagcggatta ttagagattt atcgtccttt attaagcctg
    21901 tcgttatcag actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgag
    21961 tcgcgcttat tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt
    22021 aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg
    22081 ctgcaggatt atatcagcgg tatgaccgac ctctatgcgt gggatgaata ccgacgtctg
    22141 atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc
    22201 agaaacttta gttcggaact tcgggctata aaacgaatct gaagaacaca gcaattttgc
    22261 gttatctgtt aatcgagact gaaatacatg aaaaaaaaca cattagcact gagtgcactg
    22321 gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca
    22381 gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca
    22441 gtggtcagca ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat
    22501 ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc
    22561 tctccgttct gccagggggg ccagggcggt aatggcggcg gccagcaaca gaaattcatg
    22621 gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac
    22681 gttgttgata acgcgacggt gattaaagtc cagctgagcg atggtcgtaa gttcgacgcg
    22741 aagatggttg gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaacccgaaa
    22801 aaactgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta
    22861 gcgattggta acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg
    22921 gggcgtagcg gcctgaatgc tgaaaactac gaaaacttca tccagaccga tgcagcgatc
    22981 aaccgtggta actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac
    23041 accgcgatcc tcgcaccgga cggcggcaac atcggtatcg gttttgctat cccgagtaac
    23101 atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg
    23161 ggtattatgg ggactgagct gaattccgaa ctggcgaaag cgatgaaagt tgacgcccag
    23221 cgcggtgctt tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa
    23281 gcgggtgatg tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt
    23341 gctcaggtgg gtactatgcc ggtgggcagc aaactgaccc tgggcttact gcgcgacggt
    23401 aagcaggtta acgtgaacct ggaactgcag cagagcagcc agaatcaggt tgattccagc
    23461 tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg
    23521 gtagtgaaca acgtgaagac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat
    23581 gtgattattg gtgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc
    23641 gacagcaaac cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg
    23701 ttaatgcagt aatctccctc aaccccttcc tgaatacggg aaggggttct ccttacaatc
    23761 tgtgaacttc gccacaactc catacatctt catcatcctt tgggcatttg cacaatgccg
    23821 tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca
    23881 tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa
    23941 tatcaacgta atggatgccc gcgggcgaat tatcggcagc ggcgatcgtg agcgtattgg
    24001 tgaattgcac gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga
    24061 cgcggtagca cgtcatctgc acggtgtgcg gcaggggatt aatctaccgt tacggctgga
    24121 aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg
    24181 cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacttgtt
    24241 ggcgcaggat agccgtttgc gggaagaact ggtgatgaac ctgattcagg cagaggagaa
    24301 tactcccgca cttctgaatg ggcgcaacgg ctggggatcg atctcaatca accgcgagtg
    24361 gtggctattg ttgaggtcga cagcggtcag cttggcgtgg acagcgcaat ggcggagtta
    24421 caacaactgc aaaacgcgct gactacgccc gagcgtaata atctggtggc gattgtctcg
    24481 ctaaccgaaa tggtggtgtt gaaaccggcg ttgaactctt ttgggcgctg ggatgcagaa
    24541 gatcatcgta agcgagttga acaactgatt acccgcatga aagagtacgg ccagctgcgt
    24601 tttcgcgttt cactgggcaa ctattttacc ggtcctggca gtattgcccg atcctatcgt
    24661 acggcgaaaa cgacgatggt ggtgggtaaa cagcggatgc cagaaagtcg ctgctatttt
    24721 tatcaggatc tgatgttacc tgtgttactc gacagtttgc gtggcgactg gcaggccaac
    24781 gaactggcgc gaccgctggc gcggctgaaa gcgatggaca ataacggctt gctgcgacga
    24841 acgctggcgg cgtggtttcg ccacaatgtg caaccgctgg caacgtcaaa ggcgttgttt
    24901 attcatcgta ataccctgga gtatcggctt aatcgtatat cggaactgac cgggcttgat
    24961 ttgggcaatt ttgatgacag gttgctgctg tatgtggcgt tacaactgga tgaagagcgg
    25021 tagattatgc gttaaggttg aggcggcgct ggctcattcc cctcagcccg aaggggagag
    25081 gggaatgccc gagttaaccg tcaattcggg attatttacg cgttagtttt tccagatcgg
    25141 cttcaatctc gctgatctta ttggttacga ctgactccag gtgacgtaag tcgtcgagga
    25201 tcttacgctt aagatcaact tcgctgcggt cacgctgaca gatttgatca agctcatcaa
    25261 tgatataccg tagattcggg ctgatttcct ggacttcttt ataaccctga cccacaccat
    25321 cagcgacgac cgttttacgc tgacgcggat atttaaactt aacgctcttg gcgaaaaact
    25381 cgcctttgtc tttctggaaa tagattttca gaatatcgtt gttggcttcc tgccggaggc
    25441 tgtaacgatc aatttcttca ggattggtaa tacccagact tttcagattg tcgtacatag
    25501 cgttacctca aaatgagtca gtaaattgtg cttattttag catttggctt ggcccgcccg
    25561 gctcgactgt gatcgcagaa agcctttttc ttccagactt ctatcatggc gtaaaaatca
    25621 aaaaattacc tgctttattg tggcgatatt ccatgggatg gattagcaca tttatcttcg
    25681 ttttgcgaat tacatgacag tccgaaacaa gatgaaaaag tatttaaaaa agggggagga
    25741 agaatgagta ggacgaatta ttctgagcca gaaagcatac gggcagcatc atgctgcccg
    25801 tatatgtact cttaatcgat ggtacgcagc agttcgttaa tgccgacttt gccgcgagtt
    25861 ttcgcgtcaa ctttcttaac gataaccgca cagtagaggc tgtatttgcc atcttttgac
    25921 ggcagattac ctgaaacaac cacagacccc gccggaacgc gaccgtagtg gatttcgccg
    25981 gtttcacggt cgtaaatacg ggtgctctga ccaatgtata cgcccataga gatgacggaa
    26041 ccttcttcga caatcacccc ttcaaccact tcagagcgtg cgccaatgaa gcagttatct
    26101 tcaataatgg tcgggttagc ctgcagcggc tccagcacgc cgccgatgcc cacgccaccg
    26161 gaaaggtgga cgtttttacc aatctgcgca caagaaccga cggtcgccca ggtatcaacc
    26221 atggtgcctt catcaacata tgcgccgatg ttaacgtaag acggcatcag cacggtgtta
    26281 cgggcaataa acgcaccctg acgtaccgcc gctggtggca caacgcggaa gccttctttc
    26341 tggaaacgtg cttcgtcgta gtcggcgaat ttcatcggca ctttgtcgaa gtagcggctt
    26401 tctgcccctt cgatcacctg attatcatta atacggaaag agagcagcac cgcttttttc
    26461 aaccactgat gcgtcaccca ctgaccgtca attttttccg ctacacgcag tgcgccggaa
    26521 tccagcaggg cgatcacctg atttaccgct tcgcgggtaa cggtgtctgc attggctggc
    26581 gtgatctcgg cacggcgttc aaaagcggtt tcaataatgt tctgtaactg ctgcattgtt
    26641 aaactctttt catatcagta aacacatcac cctttatcgt ttggattgag ggcctctgtc
    26701 aaccgctgat gcacttcctg ctgcaactcg ttattaagcg cacgccggtc agcggtggca
    26761 attatgaata aatcttctac tcgctcgcca atggttgtaa ttcgggcacc atgaagcgaa
    26821 attcccagat cggcaaaaat tttcccgact cgcgccagca gtccaggttg gtcgagggcg
    26881 atcagttcga ggaacgattt gcggtcggta tgggtcggca aaaacgttac ttcggtttca
    26941 acagtaaaat ggcgtaattt ggcgggttgg cgacggggct gcggtggctg ccagctactt
    27001 tgcgtcagca cctgctccag accaaaccga ataacctcat ggcgatctgc ggacagcggg
    27061 ctgccatcgg gttccagcac gataaaggta tccatcgcca taccgtcgcg agtggtgaaa
    27121 atttgtgcgt cgtgaacact taaattgcgg cggtctaatt cggcacagac ggcggcaaac
    27181 agataggggc ggtccgggct ccagataaaa atctcggtgc ctccacgcgt agcctgcggg
    27241 ctaagcaata ccagcggttt gcttaaatca tgctgtaata aatggcgggc atgccaggcc
    27301 agttgatttg ggctatggcg gacaaaatag ttagcacgac agcgtgacca aatttggtgc
    27361 agcgcctctt cgtcgatgtt atccatgcgc agtagtgcca gtgcctggag ttggtgatgg
    27421 cgaacccgtt cgcgcatatc cggcgtgttt tgcattccgc gtcgtagctg cttttcggtg
    27481 gcaaaataga gttcgcgcaa cagactttgc ttccagctat tccacagtgt ttcgttggtg
    27541 gcgcaaatgt cagccactgt caggcatacc agatagcgca gacgattttc cgtttgaact
    27601 tcttcggcaa actgcttgat gacttcaggg tcctgaatat cgcggcgttg tgctgtcacc
    27661 gacatcagca agtgctggcg aaccagccag gcaaccagct gtgtttcgcg tgagttcagc
    27721 ccgtggagtt ctgcaaaatg cactacatcc tgagcaccga gaatggagtg gtcgccgccg
    27781 cgtcctttgg cgatatcgtg aaacagcgcg gcgatgaaaa tcagctcagt tgacggcagg
    27841 cgcggccaga cgtccacaca caacggatgg cgctggcgcg tttcttcact ggcaaaactc
    27901 tccagtttca gcatcacgcg gatagtatgt tcatccaccg tgtaggcgtg gaacagatca
    27961 aactgcatct gcccgacgat atgcgaccat tgcggcatat acgcgccgag cacgctatgg
    28021 cgatgcattg gcaatagtcc gcgccgcacc gctccggggt gacgcagaat gctcaaaaac
    28081 agttttcgtg cttgcagaat attacacagc ggttgttgca gatggcgacg ggcatggcgt
    28141 aactggcgca gcgtggtgga gtaaatgccg gtgatcgcac tgttgcgcac catggtgtag
    28201 aacatacgca agatggcttc cggctggcgc ataaatagtg tttcatcacg caggtcgatt
    28261 agcgtaccgc gtagctgaaa ctcatcgtcg agtggacgtg gtttttcgtc ggcgggaagg
    28321 gcgaggatgg cttcatcgaa cagttgcagc agcatctggt tgagttcact gacgcggcgt
    28381 gtaacgcgga agtaatcctt catcatccgc tcgaccggtt cgttgccttc accactgtaa
    28441 ttcagacgct gagcgacgct aagttggcga tcgaataaca gacgattatc gtaacggctg
    28501 acgaccagat gcagggcaaa gcgaatacgc cacagtatat gcagacattc gtttaattcc
    28561 gcccgctccg ctgaggttaa gaagccaaac ccgaccattt catccagcga tgttgcgcca
    28621 aaatgacggc gggccaccca ttgcagagtg tgaatatcgc gcaagccgcc agggctactt
    28681 ttgatgtctg gttcaaggtt gtagctggtg ccatggtaac gctgatggcg ctggttctgt
    28741 tcttcaactt tcgctgcgta gaacttgtcg gaaggccaga atccttcgct aaaaatatgt
    28801 ttttgcagtt cgaggaagag cgcaacatcg ccaattaata agcgggattc gattaaattg
    28861 gtggcgacgg ttaaatccga taacccttcc aacatgcact cttcaagcgt gcgcacacta
    28921 tgaccgactt ccagctttac atcccagagc agcgttaaca gctcgcccac tttttgcgcc
    28981 tgatcgtccg ggagcttttt acggcttaaa atcagtaaat cgatgtcaga aagtggatgc
    29041 agctcgccac gaccgtagcc accgacggca accaatgcca ggtcggcaat ctggctgaat
    29101 cccgcttcaa tccataatcg ttgcaggagc tggtctatga actcggtacg tgcctcaatc
    29161 aactgttctg cagagatccc attgtcaaag gcatcaccca gccaacgctg gaaagtatcg
    29221 atatgggctt ttatcccaca gacggttaat tcatcacggg gccaggcgca tggattttgc
    29281 ggttgaccgg gcagggtgga gagagcggtg tttgcgtact gttctggaag ggtattcatt
    29341 gtgcgccacc cataagatta aattatcgca ttaaaaaagc cggcattcgc cggcttcatc
    29401 ttattcgtcg tgcgagatta tcgccgggat ggtgtcatcc ttgcgtagcg tcaaaatttc
    29461 gcagccgtta tcagtcacca caatagtatg ctcatattgt gcagacaagc tgcgatcttt
    29521 ggtttttacc gtccagccat ctttcatggt gcggatctct tttttacccg cgttgaccat
    29581 tggctcaatg gtgaacgtca tccccggttt cagcacgacg ttggtttcac gggagtcata
    29641 gtgcagcacc tgcggttctt catggaagcc gcgaccaata ccgtgtccgc aatattcacg
    29701 aacgacggag aagccttctg cttcgacaaa tttctgaatc gccgcaccga tttcgcgcag
    29761 attaatgcct ggttttacca tgcgtagcgc caggtacagg ctttcttgcg tgatgcggca
    29821 caggcgttcg cccatgatgg tcggcttacc gacgataaac attttcgagg tatcgccgtg
    29881 gaaaccatct ttgattacgg tgacatcaat gttaacgata tcgccatctt tcagcagctt
    29941 agcatcgtcc gggataccgt ggcacaccac ttcattaata gagatgcaaa cggatttcgg
    30001 atagccgtga tagccgaggc aggcagaaac cgcgtgttgt tcattaacaa tgtaatcatt
    30061 acagatgcga tccagctcgc cggtgctgac gcccggttta acatacggtt cgatcatctc
    30121 cagcacttcg gcagccagtc ggccagcgac gcgcattttt tcgatatctt ctggggtctt
    30181 gattgagata gccattaatt ctgtccatca gcgtcggtga taccgacaat atatatgtaa
    30241 gtgccgtcaa tggtatcaca cccgggcaaa ttgagaatca ttctgaattt cgccaaacgt
    30301 gccactgaag gttttctata atagaaaatt cgacgtctga tgctgtacac agcgccaaca
    30361 attattggtg tccacgacgt atttgtggta taaagcgcgc cggacttccg atccatttcg
    30421 tatacacaga ctggacggaa gcgacaatct cactttgtgt aacaacacac acgtatcggc
    30481 acatattccg gggtgccctt tggggtcggt aatatgggat acgtggaggc ataaccccaa
    30541 cttttatata gaggttttaa tcatggcaac tgtttccatg cgcgacatgc tcaaggctgg
    30601 tgttcacttc ggtcaccaga cccgttactg gaacccgaaa atgaagccgt tcatcttcgg
    30661 tgcgcgtaac aaagttcaca tcatcaacct tgagaaaact gtaccgatgt tcaacgaagc
    30721 tctggctgaa ctgaacaaga ttgtttctcg caaaggtaaa atccttttcg ttggtactaa
    30781 acgcgctgca agcgaagcgg tgaaagacgc tgctctgagc tgcgaccagt tcttcgtgaa
    30841 ccatcgctgg ctgggcggta tgctgactaa ctggaaaacc gttcgtcagt ccatcaaacg
    30901 tctgaaagac ctggaaactc agtctcagga cggtactttc gacaagctga ccaagaaaga
    30961 agcgctgatg cgcactcgtg agctggagaa actggaaaac agcctgggcg gtatcaaaga
    31021 catgggcggt ctgccggacg ctctgtttgt aatcgatgct gaccacgaac acattgctat
    31081 caaagaagca aacaacttgg gtattccggt atttgctatc gttgatacca actctgatcc
    31141 ggacggtgtt gacttcgtta tcccgggtaa cgacgacgca atccgtgctg tgaccctgta
    31201 cctgggcgct gttgctgcaa ccgtacgtga aggccgttct caggatctgg cttcccaggc
    31261 ggaagaaagc ttcgtagaag ctgagtaata aggcttgata actcccccaa aatagttcga
    31321 gttgcagaaa ggcggcaagc tcgagaattc ccgggagctt acatcagtaa gtgaccggga
    31381 tgagcgagcg aagacaacgc atctgcggcg cgaaatatga agggggagag cccttataga
    31441 ccaggtagta cacgtttggt tagggggcct gcatatggcc ccctttttca cttttatatc
    31501 tgtgcggttt aatgccgggc agatcacatc tccgaggatt ttagaatggc tgaaattacc
    31561 gcatccctgg taaaagagct gcgtgagcgt actggcgcag gcatgatgga ttgcaaaaaa
    31621 gcactgactg aagctaacgg cgacatcgag ctggcaatcg aaaacatgcg taagtccggt
    31681 gctattaaag cagcgaaaaa agcaggcaac gttgctgctg acggcgtgat caaaaccaaa
    31741 atcgacggca actacggcat cattctggaa gttaactgcc agactgactt cgttgcaaaa
    31801 gacgctggtt tccaggcgtt cgcagacaaa gttctggacg cagctgttgc tggcaaaatc
    31861 actgacgttg aagttctgaa agcacagttc gaagaagaac gtgttgcgct ggtagcgaaa
    31921 attggtgaaa acatcaacat tcgccgcgtt gctgcgctgg aaggcgacgt tctgggttct
    31981 tatcagcacg gtgcgcgtat cggcgttctg gttgctgcta aaggcgctga cgaagagctg
    32041 gttaaacaca tcgctatgca cgttgctgca agcaagccag aattcatcaa accagaagac
    32101 gtatccgctg aagtggtaga aaaagaatac caggtacagc tggatatcgc gatgcagtct
    32161 ggtaagccga aagaaatcgc agagaaaatg gttgaaggcc gcatgaagaa attcaccggc
    32221 gaagtttctc tgaccggtca gccgttcgtt atggaaccaa gcaaaactgt tggtcagctg
    32281 ctgaaagagc ataacgctga agtgactggc ttcatccgtt tcgaagtggg tgaaggcatc
    32341 gagaaagttg agactgactt tgcagcagaa gttgctgcga tgtccaagca gtcttaatta
    32401 tcaaaaagga gccgcctgag ggcggcttct ttttgtgccc atcttgtaaa ttcagctaac
    32461 ccttgtgggg ctgcgctgaa aagcgacgta caatgtcgct agtattaatt catttcaatc
    32521 gttgacagtc tcaggaaaga aacatggcta ccaatgcaaa acccgtctat aaacgcattc
    32581 tgcttaagtt gagtggcgaa gctctgcagg gcactgaagg cttcggtatt gatgcaagca
    32641 tactggatcg tatggctcag gaaatcaaag aactggttga actgggtatt caggttggtg
    32701 tggtgattgg tgggggtaac ctgttccgtg gcgctggtct ggcgaaagcg ggtatgaacc
    32761 gcgttgtggg cgaccacatg gggatgctgg cgaccgtaat gaacggcttg gcaatgcgtg
    32821 atgcactgca ccgcgcctat gtgaacgctc gtctgatgtc cgctattcca ttgaatggcg
    32881 tgtgcgacag ctacagctgg gcagaagcta tcagcctgtt gcgcaacaac cgtgtggtga
    32941 tcctctccgc cggtacaggt aacccgttct ttaccaccga ctcagcagct tgcctgcgtg
    33001 gtatcgaaat tgaagccgat gtggtgctga aagcaaccaa agttgacggc gtgtttaccg
    33061 ctgatccggc gaaagatcca accgcaacca tgtacgagca actgacttac agcgaagtgc
    33121 tggaaaaaga gctgaaagtc atggacctgg cggccttcac gctggctcgt gaccataaat
    33181 taccgattcg tgttttcaat atgaacaaac cgggtgcgct gcgccgtgtg gtaatgggtg
    33241 aaaaagaagg gactttaatc acggaataat tcccgtgatg gataaataag ggtaagattc
    33301 cgcgtaagta tcgcgggggc gtaagtctgg ttataaggcg ttattgttgc aggcagtttg
    33361 gtcacggcca gcgcgcagca accggagcgt acaaaagtac gtgaggatgg cgagcactgc
    33421 tcggggccaa aatggcaaat aaaatagcct aataatccag acgattaccc gtaatatgtt
    33481 taatcagggc tatacttagc acacttccac tgtgtgtgac tgtctggtct gactgagaca
    33541 agttttcaag gattcgtaac gtgattagcg atatcagaaa agatgctgaa gtacgcatgg
    33601 acaaatgcgt agaagcgttc aaaacccaaa tcagcaaaat acgcacgggt cgtgcttctc
    33661 ccagcctgct ggatggcatt gtcgtggaat attacggcac gccgacgccg ctgcgtcagc
    33721 tggcaagcgt aacggtagaa gattcccgta ctctgaaaat caacgtgttt gatcgttcaa
    33781 tgtctccggc cgttgaaaaa gcgattatgg cctccgatct cggcctgaac ccgaactctg
    33841 cgggtagcga catccgtgtt ccgctgccgc cgctgacgga agaacgtcgt aaagatctga
    33901 ccaaaatcgt tcgtggtgaa gcagaacaag cgcgtgttgc agtacgtaat gtgcgtcgtg
    33961 acgcgaacga caaagtgaaa gcactgttga aagataaaga gatcagcgaa gacgacgatc
    34021 gccgttctca ggacgatgta cagaaactga ctgatgctgc aatcaagaaa attgaagcgg
    34081 cgctggcaga caaagaagca gaactgatgc agttctgatt tcttgaacga caaaaacgcc
    34141 gctcagtaga tccttgcgga tcggctggcg gcgttttgct ttttattctg tctcaactct
    34201 ggatgtttca tgaagcaact caccattctg ggctcgaccg gctcgattgg ttgcagcacg
    34261 ctggacgtgg tgcgccataa tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa
    34321 aatgtcactc gcatggtaga acagtgcctg gaattctctc cccgctatgc cgtaatggac
    34381 gatgaagcga gtgcgaaact tcttaaaacg atgctacagc aacaggggag ccgcaccgaa
    34441 gtcttaagtg ggcaacaagc cgcttgcgat atggcagcgc ttgaggaggt tgatcaggtg
    34501 atggcagcca ttgttggtgc tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt
    34561 aaaaccattt tgctggccaa taaagaatca ctggttacct gcggacgtct gtttatggac
    34621 gccgtaaagc agagcaaagc gcaattgtta ccggtcgata gcgaacataa cgctattttt
    34681 cagagtttac cgcaacctat ccagcataat ctgggatacg ctgaccttga gcaaaatggc
    34741 gtggtgtcca ttttacttac cgggtctggt ggccctttcc gtgagacgcc attgcgcgat
    34801 ttggcaacaa tgacgcccga tcaagcttgc cgtcatccga actggtcgat gggacgtaaa
    34861 atttctgtcg attcggctac catgatgaac aaaggtctgg aatacattga agcgcgttgg
    34921 ctgtttaacg ccagcgccag ccagatggaa gtgctgattc acccgcagtc agtgattcac
    34981 tcaatggtgc gctatcagga cggcagtgtt ctggcgcagc tgggggaacc ggatatgcgt
    35041 acgccaattg cccacaccat ggcatggccg aatcgcgtga actctggcgt gaagccgctc
    35101 gatttttgca aactaagtgc gttgacattt gccgcaccgg attatgagcg ttatccatgc
    35161 ctgaaactgg cgatggaggc gttcgaacaa ggccaggcag cgacgacagc attgaatgcc
    35221 gcaaacgaaa tcaccgttgc tgcttttctt gcgcaacaaa tccgctttac ggatatcgct
    35281 gcgttgaatt tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat
    35341 gtgttatctg ttgatgcgaa cgcgagtgaa gtcgccagaa aagaggtgat gcgtctcgca
    35401 agctgaggat aatccggcta cagagagtcg cgctatttgt tagcgtaggg cttcagtgat
    35461 atagtctgcg ccatctgatc gtaagtagtt ggctttataa ggtcaggtat gccgtggttt
    35521 tacacggctt ttttttgtat aggcttcagt attcctgagt accgtaaacc ctgtcaggga
    35581 ataaaaaacg cgtgatgttg tctgctactc aaccacttag cgaaaaattg ccagcgcatg
    35641 gctgccgtca tgttgcgatc attatggacg gcaatggtcg ctgggcaaaa aagcaaggga
    35701 agattcgtgc ctttgggcat aaagccgggg caaaatccgt ccgccgggct gtctcttttg
    35761 cggccaacaa cggtattgag gcgttaacgc tgtatgcctt tagtagtgaa aactggaacc
    35821 gaccagcgca ggaagtcagt gcgttaatgg aactgtttgt gtgggcgctc gatagcgaag
    35881 taaaaagtct gcaccgacat aacgtgcgtc tgcgtattat tggcgatacc agtcgtttta
    35941 actcgcgttt gcaagaacgt attcgtaaat ctgaagcgct aacagccgga aataccggtc
    36001 tgacgctgaa tattgcggcg aactacggtg gacgttggga tatagtccag ggagtcaggc
    36061 aactggctga aaaggtgcag caaggaaacc tgcaaccaga tcagatagat gaagagatgt
    36121 taaaccagca tgtctgtatg catgaactgg cccctgtaga tttagtaatt aggactgggg
    36181 gagagcatcg cattagtaac tttttgcttt ggcaaattgc ctatgccgaa ctttacttta
    36241 cagatgttct ctggcccgat ttcgatgaac aagactttga aggggcgtta aatgcctttg
    36301 ctaatcgaga gcgtcgtttc ggcggcaccg agcccggtga tgaaacagcc tgatgggggt
    36361 cgcttttgct gaagtatcgc ctgatatctg cttttgtgtt aatacccgtc gtcatcgcgg
    36421 cgttgtttct gttgccgccg gtggggttcg ccattgtaac gctggtggtc tgcatgctgg
    36481 cagcgtggga atggggacag cttagcggtt ttaccactcg ttcgcagcga gtatggttgg
    36541 cggtgttatg cgggttattg ttggcgctga tgctttttct gttgccggaa tatcaccgaa
    36601 atattcatca accgctggtt gaaatctcac tttgggcttc gttgggttgg tggattgtcg
    36661 cgctattgct ggtgctgttt tacccaggtt ccgcagcaat ctggcgtaac tctaaaacat
    36721 tgcgccttat ttttggcgtg ctaaccattg ttcccttctt ctggggcatg ctggcgttac
    36781 gggcctggca ctatgacgag aatcattaca gtggcgcaat atggctgctc tatgtcatga
    36841 tcctggtatg gggcgctgac tccggcgcat atatgtttgg caaattgttt ggtaaacata
    36901 agctggcacc gaaggtttct ccgggtaaaa cctggcaagg ctttataggt ggactcgcta
    36961 ctgcagcggt aatctcatgg ggttatggca tgtgggcgaa tctcgacgtc gctcccgtca
    37021 ccttactcat ttgctctatt gtcgcagcgt tagcctcagt gctcggcgat ctgaccgaga
    37081 gtatgtttaa gcgtgaagca ggaattaagg acagcggtca tttaattcca ggacacggtg
    37141 gtattttaga tcgtattgat agcctgacgg ctgcggtacc ggtctttgct tgcttgttgt
    37201 tactggtatt caggacgctt taacggaagg taatatgctg agttttctct gggatttggc
    37261 ttcgttcatc gttgcactgg gtgtacttat caccgtgcat gaatttggtc atttctgggt
    37321 tgcccggcgt tgtggtgttc gcgttgagcg tttctcaata gggtttggta aggcgctctg
    37381 gcggcgaact gataagctcg gcaccgaata tgttatcgcc ctgatcccgt tgggcggtta
    37441 tgtcaaaatg ctggatgagc gcgcagaacc ggtcgttccg gaactccgcc accatgcctt
    37501 caataataaa tctgtcggcc aacgagcggc gattattgcc gcaggtccgg ttgcaaactt
    37561 catttttgct atctttgcct actggctggt ttttattatt ggtgtgcctg gcgtacgtcc
    37621 ggtggttggc gaaatagcag ccaattcgat agctgcggaa gcacaaattg caccaggtac
    37681 ggaactaaaa gccgtagatg gtatcgaaac gcctgattgg gatgccgtgc gtttgcagtt
    37741 ggtcgataaa attggcgatg aaagcaccac cattacagta gcgccatttg acagcgacca
    37801 acggcgggat gtaaagctcg atttacgtca ctgggcgttt gagcctgata aagaagatcc
    37861 ggtatcttcg ctggggattc gtcctcgtgg gccgcaaatt gaacctgtac tggaaaatgt
    37921 gcagccaaac tcggcggcaa gcaaggcagg tttgcaagca ggcgacagga tcgttaaagt
    37981 cgatggtcag cccttaacgc agtgggtgac ctttgtgatg cttgtccggg ataacccggg
    38041 taaatcctta gcgttagaaa tcgaaaggca ggggagtccc ttgtctttga cattaatccc
    38101 ggagagtaaa ccgggtaatg gtaaagcgat tggttttgtc ggtattgagc cgaaagtcat
    38161 tcctttgcca gatgagtata aagttgtacg ccagtatggg ccgttcaacg ccatcgtcga
    38221 agccacggac aaaacgtggc agctgatgaa gctgacggtc agtatgctgg gaaaattgat
    38281 caccggtgat gtgaaactga acaacctcag tgggccgatc tctatcgcca agggggctgg
    38341 gatgacagcg gaactcgggg tagtttatta cctgccgttt cttgcgctta ttagcgtgaa
    38401 cttagggata attaacctgt ttccgttgcc cgtacttgac ggggggcatc tgctgttcct
    38461 tgcgatcgaa aagatcaagg gcggaccggt atccgagcgg gttcaagact tttgttatcg
    38521 cattggctcg attctgctgg tgctgttaat ggggcttgca cttttcaatg atttctctcg
    38581 gttatgagag ttagttagga agaacgcata ataacgatgg cgatgaaaaa gttgctcata
    38641 gcgtcgctgc tgtttagcag cgccaccgta tacggtgctg aagggttcgt agtgaaagat
    38701 attcatttcg aaggccttca gcgtgtcgcc gttggtgcgg ccctcctcag tatgccggtg
    38761 cgtacaggcg acacggttaa tgatgaagat atcagtaata ccattcgcgc tctgtttgct
    38821 accggcaact ttgaggatgt tcgcgtcctt cgtgatggtg atacccttct ggttcaggta
    38881 aaagaacgtc cgaccattgc cagcattact ttctccggta acaaatcggt gaaagatgac
    38941 atgctgaagc aaaacctcga ggcttctggt gtgcgtgtgg gcgaatccct cgatcgcacc
    39001 accattgccg atatcgagaa aggtctggaa gacttctact acagcgtcgg taaatatagc
    39061 gccagcgtta aagctgtcgt gaccccgctg ccgcgcaacc gtgttgacct aaaactggtg
    39121 ttccaggaag gtgtgtcagc tgaaatccag caaattaaca ttgttggtaa ccatgctttc
    39181 accaccgacg aactgatctc tcatttccaa ctgcgtgacg aagtgccgtg gtggaatgtg
    39241 gtaggcgatc gtaaatacca gaaacagaaa ctggcgggcg atcttgaaac cctgcgcagc
    39301 tactatctgg accgcggtta tgcccgtttc aacatcgact ctacccaggt cagtctgacg
    39361 ccagataaaa aaggtattta cgtcacggtg aacatcaccg aaggcgatca gtacaagctt
    39421 tctggcgttg aagtgagcgg caaccttgcc gggcactccg ctgaaattga gcaactgact
    39481 aagatcgagc cgggcgagct gtataacggc actaaagtga ccaaaatgga agatgacatt
    39541 aaaaagcttc tcggtcgcta tggttatgcc tatccgcgcg tacagtcgat gcctgaaatt
    39601 aacgatgccg acaaaaccgt taaattacgc gtgaacgttg atgcgggtaa ccgtttctac
    39661 gtgcgtaaga tccgttttga aggtaacgat acctcgaaag atgccgtcct gcgtcgcgaa
    39721 atgcgtcaga tggaaggtgc atggttgggg agcgatctgg tcgatcaggg taaggagcgt
    39781 ctgaatcgtc tgggcttctt tgaaactgtc gataccgata cccaacgtgt tccgggtagc
    39841 ccggaccagg ttgatgtagt ctacaaggtg aaagaacgta acactggtag cttcaacttc
    39901 gggattggtt acggtactga aagtggcgtg agcttccagg ctggcgtgca acaggataac
    39961 tggttaggta caggttatgc tgttggtatc aacgggacca aaaacgatta ccagacctat
    40021 gctgaactgt cggtaaccaa cccgtacttc accgtagatg gcgtaagcct cggtggtcgt
    40081 ctcttctata atgacttcca ggcagatgac gccgacctgt ccgactatac caacaagagt
    40141 tatggtacag acgtgacgtt gggcttcccg attaacgaat ataactcgct gcgtgcaggt
    40201 ctgggttatg tacataactc cctgtccaac atgcagcctc aggttgcgat gtggcgttat
    40261 ctgtactcta tgggtgaaca tccgagcacc tctgatcagg ataacagctt caaaacggac
    40321 gacttcacgt tcaactatgg ttggacctat aacaagcttg accgtggtta cttcccgaca
    40381 gatggttcac gtgtcaacct gaccggtaaa gtgaccattc ctggatcgga taacgaatac
    40441 tacaaagtga cgttagacac ggcgacttat gtgccgatcg atgacgatca caaatgggtt
    40501 gttctgggtc gtacccgctg gggttatggt gatggtttag gcggcaaaga gatgccgttc
    40561 tacgagaact tctatgccgg tggttccagc accgtgcgtg gcttccagtc caataccatt
    40621 ggtccgaaag cagtttactt cccgcatcag gccagtaatt atgatccgga ctatgattac
    40681 gaatgtgcga ctcaggacgg cgcgaaagac ctgtgtaaat cggatgatgc tgtaggcggt
    40741 aacgccatgg cggttgccag cctcgagttc atcaccccga cgccgtttat tagcgataag
    40801 tatgctaact cggttcgtac ttcattcttc tgggatatgg gtaccgtttg ggatacaaac
    40861 tgggattcca gccaatattc tggatatccg gactatagtg atccaagcaa tatccgtatg
    40921 tctgcgggta tcgcattaca atggatgtcc ccattggggc cgttggtgtt ctcctacgcc
    40981 cagccgttca aaaagtacga tggagacaag gcagaacagt tccagtttaa catcggtaaa
    41041 acctggtaag tgttctccac aaaggaatgt agtggtagtg tagcgatgac tttaggcgat
    41101 caatataaga tcgccgggcc acgcaaagaa ctgcaccctc cggtgcaaat gggatggtaa
    41161 ggagtttatt gtgaaaaagt ggttattagc tgcaggtctt ggtttagcac tggcaacttc
    41221 tgctcaggcg gctgacaaaa ttgcaatcgt caatatgggc agcctgttcc agcaggtagc
    41281 gcagaaaacc ggtgtttcta acacgctgga aaatgagttc aaaggccgtg ccagcgaact
    41341 gcagcgtatg gaaaccgatc tgcaggctaa aatgaaaaaa ctgcagtcca tgaaagcggg
    41401 cagcgatcgc accaaactgg aaaaagacgt gatggctcag cgccagactt ttgctcagaa
    41461 agcgcaggct tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg gcaaactggt
    41521 tactcgtatc cagactgctg tgaaatccgt tgccaacagc caggatatcg atctggttgt
    41581 tgatgcaaac gccgttgctt acaacagcag cgatgtaaaa gacatcactg ccgacgtact
    41641 gaaacaggtt aaataagtaa tgccttcaat tcgactggct gatttagcgc agcagttgga
    41701 tgcagaacta cacggtgatg gcgatatcgt catcaccggc gttgcgtcca tgcaatctgc
    41761 acaaacaggt cacattacgt tcatggttaa cccaaaatac cgtgagcatt taggcttgtg
    41821 ccaggcgtcc gcggttgtca tgacccagga cgatcttcct ttcgcgaaaa gtgccgcgct
    41881 ggtagtgaag aatccctacc tgacttacgc gcgcatggcg caaattttag ataccacgcc
    41941 gcagcccgcg cagaacattg cacccagtgc ggtgatcgac gcgacggcga agctgggtaa
    42001 caacgtatcg attggcgcta acgcggtgat tgagtccggc gttgaactgg gcgataacgt
    42061 gattatcggt gccggttgct tcgtaggtaa aaacagcaaa atcggtgcag gttcgcgtct
    42121 ctgggcgaac gtaaccattt accatgagat ccagatcggt cagaattgcc tgatccagtc
    42181 cggaacagtg gtaggcgcag acggctttgg ttatgccaac gatcgtggta actgggtgaa
    42241 gatcccacag attggtcgcg taattattgg cgatcgcgtg gagatcggtg cctgcacaac
    42301 catcgatcgc ggcgcgctgg atgacactgt tattggcaat ggcgtgatca ttgataacca
    42361 gtgccagatt gcacataacg tcgtgattgg cgacaatacg gcggttgccg gtggcgtcat
    42421 tatggcgggc agcctgaaaa ttggtcgtta ctgcatgatc ggcggagcca gcgtaatcaa
    42481 cgggcatatg gaaatatgcg acaaagtgac ggttacgggc atgggtatgg tgatgcgtcc
    42541 catcactgaa ccaggcgtct attcctcagg cattccgctg caacccaaca aagtctggcg
    42601 caaaaccgct gcactggtga tgaacattga tgacatgagc aagcgtctga aatcgcttga
    42661 gcgcaaggtt aatcaacaag actaacgttc catcttttgt tcgccaaact ttacggcctg
    42721 tctcattctt acgattgcgg caggccgtgt tattattgtc gtttcttata ttttgacagg
    42781 aagagtatct tgactactaa cactcatact ctgcagattg aagagatttt agaacttctg
    42841 ccgcaccgtt tcccgttctt actggtggat cgcgtgctgg attttgaaga aggtcgtttt
    42901 ctgcgcgcag taaaaaatgt ctctgtcaat gagccattct tccagggcca tttccctgga
    42961 aaaccgattt tcccgggtgt gctgattctg gaagcaatgg cacaggcaac aggtattctg
    43021 gcgtttaaaa gcgtaggaaa actggaaccg ggtgagctgt actacttcgc tggtattgac
    43081 gaagcgcgct tcaagcgccc ggtcgtgcct ggcgatcaaa tgatcatgga agtcactttc
    43141 gaaaaaacgc gccgcggcct gacccgtttt aaaggggttg ctctggtcga tggtaaagta
    43201 gtttgcgaag caacgatgat gtgtgctcgt agccgggagg cctgatacgt gattgataaa
    43261 tccgcctttg tgcatccaac cgccattgtg gaagagggcg cgtcaattgg cgcgaacgca
    43321 cacattggtc ctttttgtat cgttggaccc catgtcgaaa ttggtgaggg taccgtactg
    43381 aaatctcacg ttgtcgtgaa tggtcatact aaaattggcc gcgataatga gatttatcag
    43441 tttgcctcca tcggcgaagt taaccaggat ttgaaatatg ctggcgaacc gacccgtgtg
    43501 gaaatcggcg atcgtaaccg cattcgcgaa agcgtcacca ttcatcgtgg cacagtccag
    43561 ggcggtggat tgacgaaggt gggcagcgac aacttactga tgatcaacgc gcacattgcg
    43621 cacgattgta cggtaggtaa ccgctgtatt ctcgccaaca acgcaacgct ggcgggtcac
    43681 gtatcggttg acgacttcgc gatcatcggc ggcatgaccg cagtccatca gttctgcatc
    43741 attggtgcgc atgtgatggt tggcggctgc tccggtgtgg cgcaggacgt ccctccttat
    43801 gttattgcgc agggtaacca cgcaacgccg ttcggtgtca atatcgaagg gctgaagcgc
    43861 cgcggattca gccgtgaggc gattaccgct atccgcaatg cgtataagct aatttatcgt
    43921 agcggtaaaa cgctcgatga agtgaaaccg gaaattgctg aactggcgga aacatatccg
    43981 gaagtgaaag cctttaccga tttctttgca cgctcaacgc gcggtctgat tcgttaatga
    44041 ctgaccagcg tccattaacg attgccctgg tcgccggaga aacctccggc gatatcctgg
    44101 gggccggttt aatccgcgct ctgaaagaac gtgtgcccaa cgcccgcttt gttggtgttg
    44161 ccgggccacg aatgcaggct gaaggctgcg aagcctggta cgaaatggaa gaactggcgg
    44221 tgatgggcat tgttgaagtg ctcggtcgtc tgcgtcgctt actgcatatt cgtgccgatc
    44281 tgacaaagcg ttttggcgaa ctgaagccag atgtttttgt tggtattgat gcgcccgact
    44341 tcaatattac tcttgaaggt aacctcaaaa agcagggtat caaaaccatt cattacgtca
    44401 gtccatccgt ctgggcgtgg cgacagaaac gcgttttcaa aataggcaga gccaccgatc
    44461 tggtgctcgc atttctgcct ttcgaaaaag cgttttatga caaatacaat gtaccgtgcc
    44521 gctttatcgg tcataccatg gctgatgcca tgccgttaga tccagataaa aatggtgccc
    44581 gtgatgtgct ggggatccct tacgatgccc actgtctggc attgttgccg ggcagccgtg
    44641 gtgcagaagt cgaaatgctt agtgccgatt tcttgaaaac tgcccagctt ttgcgccaga
    44701 catatccgga tctcgaaatc gtggtgccgc tggtgaatgc caaacgccgc gagcagtttg
    44761 aacgcatcaa agctgaagtc gcgccagacc tgtcggttca tttgctggat ggaatgggcc
    44821 gtgaggcgat ggtcgccagc gatgcggcac tactggcgtc ggggacggca gccctggagt
    44881 gtatgctggc gaaatgcccg atggtggtgg gatatcgcat gaagcctttt accttctggt
    44941 tggcgaagcg gctggtgaaa actgattatg tctcgctgcc aaatctgctg gcgggcagag
    45001 agttagtcaa agagttattg caggaagagt gtgagccgca aaaactggct gcggcgctgt
    45061 taccgctgtt ggcgaacggg aaaaccagcc acgcgatgca cgataccttc cgtgaactgc
    45121 atcagcagat ccgctgcaat gccgatgagc aggcggcaca agccgttctg gagttagcac
    45181 aatgatcgaa tttgtttatc cgcacacgca gctggttgcg ggtgtggatg aagtcggacg
    45241 cgggccgtta gttggcgcgg tcgtcaccgc tgcggtgatc cttgacccgg cgcgcccgat
    45301 tgccgggctg aatgattcca aaaagctgag cgaaaaacgc cgtctggcgc tctatgaaga
    45361 gatcaaagag aaagcgttga gctggagtct gggccgcgcg gaaccccacg aaatcgacga
    45421 gctgaacatt cttcatgcga ccatgctggc gatgcagcgt gccgtcgctg ggctgcatat
    45481 tgcgccggaa tatgtgttga ttgatggtaa ccgctgcccg aaattaccga tgcctgcgat
    45541 ggctgtggtg aaaggggata gccgcgtacc agaaatcagt gccgcgtcta tcctggcgaa
    45601 agtgacgcgt gacgccgaaa tggcggcgct ggatattgtt ttcccgcaat atggttttgc
    45661 ccaacataaa gggtatccaa ccgcttttca tctggaaaaa ctggctgaat atggcgcgac
    45721 tgaacatcat cgtcgcagct ttgggcctgt caaacgcgca ctgggacttg cgtcctgatt
    45781 cttgtgtcga gattaagtaa accggaatct gaagatgtct gaaccacgtt tcgtacacct
    45841 gcgggtgcac agcgactact cgatgatcga tggcttggcc aaaaccgcac cgctggtaaa
    45901 aaaggcggcg gcgttgggta tgccagcact ggcgatcacc gatttcacca acctttgcgg
    45961 tctggtgaag ttctacggag cgggacatgg cgcagggatt aagcctatcg tcggggcaga
    46021 ttttaatgtc cagtgcgacc tgctgggtga tgagttaacc cacctgacgg tactggcggc
    46081 gaacaatacc ggctatcaga atctggcgtt gctgatctca aaagcgtatc agcgcgggta
    46141 cggtgccgcc gggccgatca tcgatcgcga ctggcttatc gaattaaacg aagggttgat
    46201 ccttctttcc ggcgggcgca tgggcgacgt cggacgcagt cttttgcgtg gtaacagcgc
    46261 gctggtagat gagtgtgtcg cgttttatga agaacacttc ccggatcgct attttctcga
    46321 gctgatccgc accggcaggc cggatgaaga aagctatctg cacgcggcgg tggaactggc
    46381 ggaagcgcgc ggtttgcccg tcgtggcgac taacgatgtg cgctttatcg acagcagcga
    46441 ctttgacgca cacgaaatcc gcgtcgcgat ccacgacggc tttaccctcg acgatcctaa
    46501 acgcccgcgt aactattcgc cgcagcaata tatgcgtagc gaagaggaga tgtgtgagct
    46561 gtttgccgac atcccagaag cccttgccaa caccgttgag atcgccaaac gctgtaacgt
    46621 aactgtgcgt cttggtgaat acttcctgcc gcagttcccg accggggaca tgagcaccga
    46681 agattatctg gtcaagcgtg caaaagaagg cctggaagag cgtctggcat ttttattccc
    46741 tgacgaggaa gaacgtctta aacgccgccc ggaatatgac gaacgtctgg agactgaact
    46801 tcaggttatc aaccagatgg gcttcccggg ctacttcctc atcgttatgg aatttatcca
    46861 gtggtcgaaa gataacggcg taccggtagg gccaggccgt ggctccggtg cgggttcact
    46921 ggtggcctac gcgctgaaaa tcaccgacct cgatccgctg gaatttgacc tgctgttcga
    46981 acgtttcctt aacccggaac gtgtctccat gcctgacttc gacgttgact tctgtatgga
    47041 gaaacgcgat caggttatcg aacacgtggc ggacatgtac ggtcgtgatg cggtatcgca
    47101 gattatcacc ttcggtacga tggcggcgaa agcggtgatc cgcgacgtag gccgcgtgct
    47161 ggggcatccg tacggctttg tcgaccgtat ctcgaaactg atcccgcccg atccggggat
    47221 gacgctggcg aaagcgtttg aagccgagcc gcagctgccg gaaatctacg aagcggatga
    47281 agaagttaag gcgctgatcg acatggcgcg caaactggaa ggggtcaccc gtaacgccgg
    47341 taagcacgcc ggtggggtgg ttatcgcgcc gaccaaaatt accgattttg cgccgcttta
    47401 ttgcgatgaa gagggcaaac atccggtcac ccagtttgat aaaagcgacg ttgaatacgc
    47461 cggactggtg aagttcgact tccttggttt gcgtacgctc accatcatca actgggcgct
    47521 ggagatgatc aacaagcggc gggcgaagaa tggcgagcca ccgctggata tcgccgcgat
    47581 cccgctggat gacaagaaaa gcttcgacat gctgcaacgc tcggaaacca cggcggtatt
    47641 ccagcttgaa tcgcgcggca tgaaggacct gatcaagcgt ctgcaacctg actgcttcga
    47701 agatatgatc gcactggtgg cactgttccg ccctggtccg ttgcagtcag ggatggtgga
    47761 taactttatc gaccgtaaac atggtcgtga agagatctcc tatccggacg tgcagtggca
    47821 gcatgaaagc ctgaaatcgg tactggagcc aacctacggc attatcttgt atcaggaaca
    47881 ggtcatgcag attgcccagg tgctttctgg ttataccctc ggtggcgcgg atatgctgcg
    47941 ccgtgcgatg ggtaagaaaa agccggaaga gatggctaag cagcgttctg tatttgctga
    48001 aggtgcagaa aagaacggaa tcaacgccga actggcgatg aaaatcttcg acctggtgga
    48061 gaaattcgcg ggttacggat ttaacaaatc gcactctgcg gcctatgctt tggtgtcata
    48121 tcaaacgtta tggctgaaag cgcactatcc ggcggagttt atggcggcgg taatgaccgc
    48181 cgatatggac aacaccgaga aggtggtggg cctggtggat gagtgctggc ggatggggct
    48241 gaaaatcctg ccaccagata taaactccgg tctttaccat ttccacgtca acgacgatgg
    48301 cgaaatcgtg tatggtattg gcgccat
//
LOCUS       DAJTMX010000014        47336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTMX010000014 DAJTMX010000000
VERSION     DAJTMX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.014"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO145_000827"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO145_000827"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5466040.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO145_000828"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO145_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5466041.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO145_000829"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO145_000829"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5466042.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO145_000830"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO145_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5466043.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO145_000831"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO145_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5466044.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO145_000832"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO145_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5466045.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO145_000833"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO145_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5466046.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO145_000834"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO145_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5466047.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO145_000835"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO145_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5466048.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO145_000836"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO145_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5466049.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO145_000837"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO145_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5466050.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO145_000838"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO145_000838"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5466051.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO145_000839"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO145_000839"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5466052.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO145_000840"
     CDS             11231..12085
                     /locus_tag="OO145_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5466053.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO145_000841"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO145_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5466054.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO145_000842"
     CDS             complement(13548..14687)
                     /locus_tag="OO145_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5466055.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO145_000843"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO145_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5466056.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO145_000844"
     CDS             15873..17234
                     /locus_tag="OO145_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5466057.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO145_000845"
     CDS             17231..17749
                     /locus_tag="OO145_000845"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5466058.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO145_000846"
     CDS             17749..18069
                     /locus_tag="OO145_000846"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5466059.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO145_000847"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO145_000847"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5466060.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20105
                     /locus_tag="OO145_000848"
     CDS             18888..20105
                     /locus_tag="OO145_000848"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5466061.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20202..20624
                     /locus_tag="OO145_000849"
     CDS             20202..20624
                     /locus_tag="OO145_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5466062.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20672..21544)
                     /locus_tag="OO145_000850"
     CDS             complement(20672..21544)
                     /locus_tag="OO145_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5466063.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21556..22650)
                     /locus_tag="OO145_000851"
     CDS             complement(21556..22650)
                     /locus_tag="OO145_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5466064.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22683..23681)
                     /locus_tag="OO145_000852"
     CDS             complement(22683..23681)
                     /locus_tag="OO145_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5466065.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23706..25217)
                     /locus_tag="OO145_000853"
     CDS             complement(23706..25217)
                     /locus_tag="OO145_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5466066.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25240..26222)
                     /locus_tag="OO145_000854"
                     /pseudo
     CDS             complement(25240..26222)
                     /locus_tag="OO145_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26319..29420)
                     /locus_tag="OO145_000855"
     CDS             complement(26319..29420)
                     /locus_tag="OO145_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5466067.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29427..29600)
                     /locus_tag="OO145_000856"
     CDS             complement(29427..29600)
                     /locus_tag="OO145_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466068.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29718..30911
                     /locus_tag="OO145_000857"
     CDS             29718..30911
                     /locus_tag="OO145_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5466069.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO145_000858"
     CDS             complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO145_000858"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5466070.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32689..33879
                     /gene="hmpA"
                     /locus_tag="OO145_000859"
     CDS             32689..33879
                     /gene="hmpA"
                     /locus_tag="OO145_000859"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5466071.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO145_000860"
     CDS             complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO145_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5466072.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO145_000861"
     CDS             complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO145_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5466073.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO145_000862"
     CDS             complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO145_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5466074.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO145_000863"
     CDS             complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO145_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5466075.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO145_000864"
                     /gene_synonym="purI"
     CDS             complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO145_000864"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5466076.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42656..44212
                     /gene="mltF"
                     /locus_tag="OO145_000865"
     CDS             42656..44212
                     /gene="mltF"
                     /locus_tag="OO145_000865"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5466077.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO145_000866"
     CDS             complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO145_000866"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5466078.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO145_000867"
     CDS             complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO145_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5466079.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45614..46462
                     /locus_tag="OO145_000868"
     CDS             45614..46462
                     /locus_tag="OO145_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5466080.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46801..47336)
                     /locus_tag="OO145_000869"
                     /pseudo
     CDS             complement(46801..47336)
                     /locus_tag="OO145_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11284 a        12967 c        11905 g        11180 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
    19441 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg
    19501 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
    19561 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
    19621 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
    19681 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
    19741 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
    19801 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
    19861 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
    19921 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
    19981 ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc
    20041 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
    20101 tgtaatcagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
    20161 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
    20221 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
    20281 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
    20341 cctcgttggg cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
    20401 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
    20461 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
    20521 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat
    20581 tggcaattac cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
    20641 ggcgtgaacg ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct
    20701 ctgaggttgt tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat
    20761 gctcatccgg cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat
    20821 caaacgcagg gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga
    20881 tgatggcata accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac
    20941 cattgttcac ccactgacgc ggcaacggcg cgagctggtt aaaatccagt tcctgcggta
    21001 gctgctcgca aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct
    21061 gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg
    21121 gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta
    21181 aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac
    21241 acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta
    21301 gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa
    21361 aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg
    21421 ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga
    21481 ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg
    21541 tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc
    21601 catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg
    21661 cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt
    21721 cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc
    21781 cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc
    21841 cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt
    21901 aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc
    21961 ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt
    22021 tgcaccgcga cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac
    22081 cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac
    22141 gccgcaactg ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc
    22201 tttttcttcc gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc
    22261 ttttcctttg ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca
    22321 gccagaaatg tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc
    22381 catcgccaca atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt
    22441 atcgggtgcc gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca
    22501 aatcccggag gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac
    22561 ttcccgcaga tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa
    22621 tccctcatta acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag
    22681 gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc
    22741 acgatgatga cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga
    22801 ccgttaccga ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga
    22861 ccgccggaaa gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg
    22921 tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc
    22981 gctaataatc ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac
    23041 aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt
    23101 cggctaatga acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca
    23161 ccgagaaatt gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt
    23221 gcgttggtca taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg
    23281 aaacttggta cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct
    23341 aacagcaaca ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc
    23401 aggcacaccg agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata
    23461 ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc
    23521 atctggttat tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg
    23581 gcaatcacca ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag
    23641 acgctcttgc cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa
    23701 attcatcagt gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga
    23761 aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat
    23821 tcctccactt cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca
    23881 atacggtaaa tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac
    23941 aaaatctggc tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca
    24001 gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc
    24061 tcttcggtca ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc
    24121 cgattggtca gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg
    24181 cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg
    24241 attttctcgc cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc
    24301 ttcagcaatt cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga
    24361 cgtagcgtaa agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt
    24421 acggcctgat ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct
    24481 agcattagcg acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc
    24541 tgaccatcgc gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg
    24601 ctgacataaa tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt
    24661 tcaacttctg cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg
    24721 cccttcatca cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca
    24781 agttgttcag gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc
    24841 atttgcaggt aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct
    24901 gccaccgtca gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca
    24961 gcggcacggc gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata
    25021 tcaccgctat ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg
    25081 ccgtttttac ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc
    25141 agcgcaacga cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc
    25201 tctgttgccg tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg
    25261 cgttgccagc cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat
    25321 cactttttca ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt
    25381 gcccattttc ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc
    25441 ctgagcaatt tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg
    25501 attacgtacc gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt
    25561 gagatccggc gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt
    25621 cccttcctgg ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa
    25681 tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg
    25741 ctggtcaatt ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat
    25801 tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata
    25861 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    25921 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    25981 tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc
    26041 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    26101 tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt
    26161 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    26221 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    26281 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    26341 aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    26401 tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    26461 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca
    26521 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    26581 tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc
    26641 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    26701 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc
    26761 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    26821 ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc
    26881 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    26941 gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc
    27001 gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    27061 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    27121 ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    27181 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca
    27241 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    27301 cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    27361 cgagcaaacc aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    27421 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    27481 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    27541 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    27601 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    27661 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    27721 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27781 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27841 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    27901 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    27961 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc
    28021 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    28081 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    28141 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    28201 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    28261 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    28321 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    28381 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    28441 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    28501 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    28561 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    28621 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    28681 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    28741 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28801 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28861 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    28921 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    28981 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    29041 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    29101 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    29161 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    29221 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    29281 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    29341 actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg
    29401 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    29461 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    29521 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    29581 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    29641 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    29701 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    29761 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29821 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    29881 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    29941 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    30001 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    30061 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    30121 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    30181 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    30241 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30301 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30361 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30421 gcggacagat tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt
    30481 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30541 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30601 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30661 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30721 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30781 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30841 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30901 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    30961 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31021 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31081 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31141 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31201 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31261 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31321 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31381 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31441 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31501 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31561 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31621 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31681 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31741 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31801 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31861 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31921 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    31981 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32041 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32101 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32161 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32221 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32281 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32341 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32401 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32461 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32521 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32581 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32641 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32701 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32761 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32821 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32881 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    32941 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33001 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33061 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33121 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33181 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33241 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33301 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33361 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33421 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33481 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33541 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33601 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33661 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33721 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33781 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33841 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33901 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    33961 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34021 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34081 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34141 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34201 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34261 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34321 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34381 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    34441 agcttacgca agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34501 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34561 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34621 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34681 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34741 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34801 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34861 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34921 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    34981 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35041 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35101 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35161 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35221 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35281 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35341 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35401 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35461 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35521 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35581 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35641 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35701 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35761 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35821 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35881 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    35941 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36001 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36061 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36121 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36181 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36241 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36301 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36361 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36421 acagcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36481 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36541 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36601 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36661 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    36721 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36781 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36841 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36901 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    36961 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37021 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37081 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37141 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37201 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37261 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37321 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37381 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37441 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37501 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37561 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37621 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37681 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37741 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37801 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37861 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37921 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    37981 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38041 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38101 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38161 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38221 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38281 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38341 cttattagat tacaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38401 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38461 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    38521 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    38581 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    38641 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    38701 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    38761 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38821 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    38881 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    38941 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    39001 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    39061 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    39121 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    39181 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    39241 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    39301 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    39361 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    39421 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    39481 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    39541 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    39601 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    39661 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    39721 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39781 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39841 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    39901 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    39961 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    40021 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    40081 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    40141 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    40201 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    40261 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    40321 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    40381 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    40441 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    40501 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    40561 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    40621 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    40681 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    40741 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40801 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40861 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    40921 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    40981 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    41041 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    41101 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    41161 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    41221 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    41281 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    41341 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    41401 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    41461 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    41521 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    41581 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    41641 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    41701 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    41761 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41821 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    41881 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    41941 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    42001 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    42061 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    42121 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    42181 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    42241 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    42301 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    42361 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    42421 cggtgtaccc aagggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa
    42481 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    42541 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    42601 cactattatt acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa
    42661 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    42721 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    42781 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    42841 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact
    42901 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc
    42961 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    43021 ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg
    43081 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa
    43141 cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa
    43201 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    43261 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga
    43321 tatcaccgat gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc
    43381 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga
    43441 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    43501 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    43561 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac
    43621 ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg
    43681 cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga
    43741 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc
    43801 tgcgtacaat atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa
    43861 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    43921 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    43981 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    44041 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    44101 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    44161 ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga
    44221 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    44281 caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc
    44341 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    44401 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc
    44461 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg
    44521 ccgcagggcc atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca
    44581 gccttcgccg attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc
    44641 atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc
    44701 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    44761 tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg
    44821 acagaaataa agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag
    44881 cggcgtgccg attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt
    44941 caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag
    45001 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    45061 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    45121 ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc
    45181 gctgtgacca aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    45241 ttttatcaat aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag
    45301 cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt
    45361 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    45421 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    45481 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    45541 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat
    45601 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca
    45661 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    45721 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    45781 aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag
    45841 ccagccggaa tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    45901 gctggtcggc gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt
    45961 taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    46021 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    46081 gattggcttc aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc
    46141 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    46201 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    46261 tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    46321 gacaaacagt gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt
    46381 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc
    46441 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    46501 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    46561 gtgaaatcaa ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt
    46621 gttgttggat ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac
    46681 tttctttaac gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac
    46741 atacaacatt aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt
    46801 ttatataacc ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat
    46861 cgccaccttc tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc
    46921 tggtgaaagt ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc
    46981 tgcttttgtt cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg
    47041 gaagaaaaag cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt
    47101 gatgcctcgt tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag
    47161 ccgatccagc ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt
    47221 cagccccggt ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc
    47281 tgcacagttc aacgcattac gttgtagatc ggtattctgg tcatttgttg atacgc
//
LOCUS       DAJTMX010000015        46415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTMX010000015 DAJTMX010000000
VERSION     DAJTMX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.015"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(133..208)
                     /locus_tag="OO145_000870"
     tRNA            complement(133..208)
                     /locus_tag="OO145_000870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Lys,seq:ttt)
     gene            complement(373..1164)
                     /gene="cpoB"
                     /locus_tag="OO145_000871"
     CDS             complement(373..1164)
                     /gene="cpoB"
                     /locus_tag="OO145_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5466081.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1174..1695)
                     /gene="pal"
                     /locus_tag="OO145_000872"
     CDS             complement(1174..1695)
                     /gene="pal"
                     /locus_tag="OO145_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5466082.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1730..3022)
                     /gene="tolB"
                     /locus_tag="OO145_000873"
     CDS             complement(1730..3022)
                     /gene="tolB"
                     /locus_tag="OO145_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5466083.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3155..4396)
                     /gene="tolA"
                     /locus_tag="OO145_000874"
     CDS             complement(3155..4396)
                     /gene="tolA"
                     /locus_tag="OO145_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5466084.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4461..4889)
                     /gene="tolR"
                     /locus_tag="OO145_000875"
     CDS             complement(4461..4889)
                     /gene="tolR"
                     /locus_tag="OO145_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5466085.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4893..5585)
                     /gene="tolQ"
                     /locus_tag="OO145_000876"
     CDS             complement(4893..5585)
                     /gene="tolQ"
                     /locus_tag="OO145_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5466086.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5582..5986)
                     /gene="ybgC"
                     /locus_tag="OO145_000877"
     CDS             complement(5582..5986)
                     /gene="ybgC"
                     /locus_tag="OO145_000877"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5466087.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6136..6429)
                     /gene="ybgE"
                     /locus_tag="OO145_000878"
     CDS             complement(6136..6429)
                     /gene="ybgE"
                     /locus_tag="OO145_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5466088.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6429..6542)
                     /gene="cydX"
                     /locus_tag="OO145_000879"
     CDS             complement(6429..6542)
                     /gene="cydX"
                     /locus_tag="OO145_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5466089.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6557..7696)
                     /gene="cydB"
                     /locus_tag="OO145_000880"
     CDS             complement(6557..7696)
                     /gene="cydB"
                     /locus_tag="OO145_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5466090.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7712..9280)
                     /gene="cydA"
                     /locus_tag="OO145_000881"
     CDS             complement(7712..9280)
                     /gene="cydA"
                     /locus_tag="OO145_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5466091.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10129..12762)
                     /gene="mngB"
                     /locus_tag="OO145_000882"
     CDS             complement(10129..12762)
                     /gene="mngB"
                     /locus_tag="OO145_000882"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5466092.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12780..14696)
                     /gene="mngA"
                     /locus_tag="OO145_000883"
     CDS             complement(12780..14696)
                     /gene="mngA"
                     /locus_tag="OO145_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5466093.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14866..15588
                     /gene="mngR"
                     /locus_tag="OO145_000884"
     CDS             14866..15588
                     /gene="mngR"
                     /locus_tag="OO145_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5466094.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15691..16560)
                     /gene="sucD"
                     /locus_tag="OO145_000885"
     CDS             complement(15691..16560)
                     /gene="sucD"
                     /locus_tag="OO145_000885"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5466095.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16560..17726)
                     /gene="sucC"
                     /locus_tag="OO145_000886"
     CDS             complement(16560..17726)
                     /gene="sucC"
                     /locus_tag="OO145_000886"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5466096.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17820..19037)
                     /gene="odhB"
                     /locus_tag="OO145_000887"
     CDS             complement(17820..19037)
                     /gene="odhB"
                     /locus_tag="OO145_000887"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5466097.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19052..21853)
                     /gene="sucA"
                     /locus_tag="OO145_000888"
     CDS             complement(19052..21853)
                     /gene="sucA"
                     /locus_tag="OO145_000888"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5466098.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22154..22870)
                     /gene="sdhB"
                     /locus_tag="OO145_000889"
     CDS             complement(22154..22870)
                     /gene="sdhB"
                     /locus_tag="OO145_000889"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5466099.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22886..24652)
                     /gene="sdhA"
                     /locus_tag="OO145_000890"
     CDS             complement(22886..24652)
                     /gene="sdhA"
                     /locus_tag="OO145_000890"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5466100.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24652..24999)
                     /gene="sdhD"
                     /locus_tag="OO145_000891"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24652..24999)
                     /gene="sdhD"
                     /locus_tag="OO145_000891"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5466101.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24993..25397)
                     /gene="sdhC"
                     /locus_tag="OO145_000892"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24993..25397)
                     /gene="sdhC"
                     /locus_tag="OO145_000892"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5466102.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25778..25885
                     /locus_tag="OO145_000893"
     CDS             25778..25885
                     /locus_tag="OO145_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466103.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26091..27374
                     /gene="gltA"
                     /locus_tag="OO145_000894"
     CDS             26091..27374
                     /gene="gltA"
                     /locus_tag="OO145_000894"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5466104.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27764..28330
                     /locus_tag="OO145_000895"
     CDS             27764..28330
                     /locus_tag="OO145_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5466105.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28381..30840
                     /locus_tag="OO145_000896"
     CDS             28381..30840
                     /locus_tag="OO145_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5466106.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30855..31586
                     /locus_tag="OO145_000897"
     CDS             30855..31586
                     /locus_tag="OO145_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5466107.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31583..32659
                     /locus_tag="OO145_000898"
                     /pseudo
     CDS             31583..32659
                     /locus_tag="OO145_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32811..33857
                     /locus_tag="OO145_000899"
     CDS             32811..33857
                     /locus_tag="OO145_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5466108.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVITAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33854..34645)
                     /gene="nei"
                     /locus_tag="OO145_000900"
     CDS             complement(33854..34645)
                     /gene="nei"
                     /locus_tag="OO145_000900"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5466109.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34681..35415)
                     /gene="pxpA"
                     /locus_tag="OO145_000901"
     CDS             complement(34681..35415)
                     /gene="pxpA"
                     /locus_tag="OO145_000901"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5466110.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35405..36337)
                     /gene="pxpC"
                     /locus_tag="OO145_000902"
                     /pseudo
     CDS             complement(35405..36337)
                     /gene="pxpC"
                     /locus_tag="OO145_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36331..36987)
                     /gene="pxpB"
                     /locus_tag="OO145_000903"
     CDS             complement(36331..36987)
                     /gene="pxpB"
                     /locus_tag="OO145_000903"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5466111.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37010..37753)
                     /gene="ybgI"
                     /locus_tag="OO145_000904"
     CDS             complement(37010..37753)
                     /gene="ybgI"
                     /locus_tag="OO145_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5466112.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38024..39505
                     /gene="dtpD"
                     /locus_tag="OO145_000905"
     CDS             38024..39505
                     /gene="dtpD"
                     /locus_tag="OO145_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5466113.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39655..41073)
                     /gene="phrB"
                     /locus_tag="OO145_000906"
     CDS             complement(39655..41073)
                     /gene="phrB"
                     /locus_tag="OO145_000906"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5466114.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41070..41579)
                     /locus_tag="OO145_000907"
     CDS             complement(41070..41579)
                     /locus_tag="OO145_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5466115.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41708..41995
                     /locus_tag="OO145_000908"
     CDS             41708..41995
                     /locus_tag="OO145_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5466116.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42050..42430)
                     /locus_tag="OO145_000909"
     CDS             complement(42050..42430)
                     /locus_tag="OO145_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466117.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42433..45819)
                     /locus_tag="OO145_000910"
     CDS             complement(42433..45819)
                     /locus_tag="OO145_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5466118.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(45838..46041)
                     /locus_tag="OO145_000911"
                     /pseudo
     CDS             complement(45838..46041)
                     /locus_tag="OO145_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11165 a        12628 c        11198 g        11424 t
ORIGIN      
        1 actacgctgg atactacaac taactatgtt agtgggggtc gtgcaggatg actcgacttc
       61 gtctcgccct tcggcgccgt tgctgatgca acgttatcct tcacgtttta ctaccgcttc
      121 caccttagcg attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
      181 actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc
      241 ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg
      301 cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc
      361 gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc
      421 agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc
      481 ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt
      541 tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa
      601 ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa
      661 attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc
      721 tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc
      781 agcatcagct gtcggcgtcg ttgatgccgt cgcaccgctt tgatcgctgc tggttgattg
      841 cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc
      901 cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc
      961 ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga
     1021 aatacgctca agttgagtga cgcggtcttc gaccgagcct gagctgacac tactgattgg
     1081 tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag
     1141 ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt
     1201 tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac
     1261 gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc
     1321 acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc
     1381 tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg
     1441 agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg
     1501 cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt
     1561 cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt
     1621 gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac
     1681 tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat cacagatacg
     1741 gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc
     1801 catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca
     1861 tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta
     1921 cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca
     1981 ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg
     2041 taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct
     2101 ggtcagaagt aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac
     2161 tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca
     2221 gactaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg
     2281 caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa
     2341 taaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg
     2401 accacgccgg agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt
     2461 catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg
     2521 caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg
     2581 cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta
     2641 cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg
     2701 gattcggagt gacttgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg
     2761 gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa
     2821 atttaccgct gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac
     2881 caggccccgc ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt
     2941 cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg
     3001 ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt
     3061 tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc
     3121 ctgatgttga ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt
     3181 ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg
     3241 ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca
     3301 taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt
     3361 catagaactt actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc
     3421 ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg
     3481 cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg
     3541 cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc gctgccgctt
     3601 ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttatcagcag
     3661 ccgctttttc tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
     3721 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc cttggctttt tcagcagctt
     3781 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
     3841 tcagtgccgc agcggctacc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
     3901 ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt cgctgcttct tccgcagctt
     3961 tagcatctgc cgccgctttc gctgccgcca cttcagcttg cttctgcttt aactcggcct
     4021 gttttgcggc ttcttcagcc tgctttttct gctcctgagc cgctaaccgc tctttctcaa
     4081 gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca gcagcctgct
     4141 gttccttcat cttgcgctgt tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc
     4201 gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg gacgaaccac
     4261 cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc agcgccgcaa
     4321 ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt
     4381 cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg ctctctttca
     4441 agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga tttcacaccc
     4501 gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc ttttgcgcca
     4561 ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc cgccaccacc
     4621 tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta ctgaccaata
     4681 ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg tgattcagta
     4741 gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc cataaagatc
     4801 agcaacagca ccagcagtac gtccagcaac ggaacaatgt tgatttcgga cttgagatcg
     4861 cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc gctaacggta
     4921 aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta attcagttcc
     4981 agtttgttta cgcgctggtt gagacggtta taagccatta ccgccggaat tgcggcgaac
     5041 agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat ttgcagtgtt
     5101 gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac cgtaccaaac
     5161 agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg cgtttccaga
     5221 ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc cactacggct
     5281 tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc gctgtaaaag
     5341 atttgttccg aaccagtcag attatcccgt ttcccctgac tctcttgata gaggcgagag
     5401 agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc cgctgcgtta
     5461 agaatacggg ttcgctggat aataatggcc caggatgcga ttgaaaaacc aatcaaaatc
     5521 aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat attcatgtca
     5581 gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc attttgagtg
     5641 ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg gcgttgacaa
     5701 tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc tgtatttcga
     5761 gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt accacaaagg
     5821 caacgcgttc agccatcagc gcctgctgac tgaagtgatg atgacgcagc atctctgtgc
     5881 gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg gcatcggtat
     5941 cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta catcccggta
     6001 atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt gaggtttggg
     6061 aatgagggca agttaagggc gcgtaaaaat ttatgggcct agagcggccc ataatttttt
     6121 aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa caatatcggc
     6181 aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg
     6241 aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt ccagttcact
     6301 ggtcttcgcg gcaaaacgag atgggtccca aaacatacat cctgctaaca gaagcgccat
     6361 cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg tcgcgataat
     6421 cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgttc aagcgccagc
     6481 gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc gaaataccac
     6541 atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct tctttggtga
     6601 tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc ggtaccagaa
     6661 ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca tcccacattg
     6721 tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt gcgataccgg
     6781 ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac gcggctttat
     6841 ccatacgtgc agtcaggatg gtcagcagca gcagaaccac acccagtgcc ggaatagccc
     6901 acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga accacttctt
     6961 tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca taaccatcga
     7021 taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc agcgcagcca
     7081 cctgagccgt tgcacgggta cgcaggtgca gttcgcccac ggtacgcatt tgcagatagg
     7141 ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg ccgaacgggt
     7201 taagcaactg gaagaagtta ccggtgtagt acagacgcag atattcatca acgttgaacg
     7261 gtacgccctg caacaggtta ccgaacgcta caccaattac cagcggcgga acgaagctac
     7321 caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc ttggagcggt
     7381 agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc gccacataga
     7441 agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg ccgcccgcgg
     7501 tgatcagcca aacctggtta ccgtcccagt gtggtgcaat ggagttaatc ataattcgac
     7561 gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc cacccccatg tcgaaaccgt
     7621 cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata aaacgcaata
     7681 cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta gtcgtggaag
     7741 actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt gcaaacttga
     7801 acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc agcaccattg
     7861 agaagatgag atcgcctgcg gtcagtgacg aattcgccac agctgtcggc agcacttcac
     7921 cgatagccca cggttggcgg ccatattcag ccacgaacca gcccgcttct acagcaatcc
     7981 acggcagcgg aataccgtac agcgcggcgc gcagaagcca ttttttctcg ccaatgcggt
     8041 tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag ccacacgcca
     8101 ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg gatggagtct ttggttgcct
     8161 gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc agcagcagac
     8221 cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgtctgg tcggtagaac
     8281 cagaacgcag ttgttcgagc agagagtacg ccttcatccc gttacgaatg cgttcttcat
     8341 gctgcaccat cagctctttc aggccgataa ccggggtatc cacggaacgc gttgcaatga
     8401 tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt ctcctcttcc tgatcaggaa
     8461 tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc ccactcggct tcaatagcag
     8521 ccagtttggt tttctgcacg tcgcccattt cgtagccgga ttcatcaccc agaacaataa
     8581 cagacagaac agcagccata ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg
     8641 cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac atcgcgccag
     8701 tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg ttaagcacca
     8761 gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg gacgcgattg
     8821 ggttttgcat ccagccgttc gcaaccagaa tccacagtgc ggacaggttt gaaccgagcg
     8881 ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa ccgaagaaga
     8941 acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt gccagcggcg
     9001 caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg aactggaact
     9061 ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac ttgccccaga
     9121 acttggtcat atctttataa atctgtttgc cggagaggac gtagaccgtt tccataatgg
     9181 ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca
     9241 aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct tgctcatcgc
     9301 atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc aatagaaatt
     9361 tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa aacggcgtaa agaaaggttg
     9421 caaacatgtt aataaaaact caaattgatc ccacgtatat attacgccgc aaaatcctta
     9481 caataaacag gtttttgttg aagcaaattt gcatttttcg acggtgatca atttatagct
     9541 aaattgccgc ctttcagcca atttgatcga gaacaattta tctctttttg atgcccattt
     9601 ccaagactta tacattgata aatatcaatt tctacaaatt atgttaatca tgtttcaaca
     9661 aggtaacttt agtgatattt atgtttaaaa tgtgtgaatt ataagactga aattagtttc
     9721 ggttggcaat taaagataaa aagagacgaa gcaaaaacag cacggttgac cttaattctt
     9781 tttttggtca ctggctaata cgacgcagag tttcaggatt attaataaag gcataaatct
     9841 cccccccgcc gatattaaca tttaattaac ttataattat attataatta actcacccgt
     9901 ggttattatt ttgttaaaaa taaagccaca tatacgtagc ttgagagtga agataaacac
     9961 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    10021 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    10081 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    10141 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    10201 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    10261 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    10321 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    10381 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    10441 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    10501 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    10561 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcctaggcc ttaaaagcag
    10621 atcttctttc cccagtaagc ccacgccgcg cagcaacgta atggcaaagg ttttcttctc
    10681 ttcaccgatg acttcaaatt cacgtagccc ttcgctaaaa acagccatgc cgttacgccc
    10741 ttcctgtaag gctgcatagt tgagcatatt ccataccgga accggcgctt ctttccagct
    10801 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    10861 ggtatctgcc agaacactat cggtgttaaa agatgtaggg atcaggacgc gaaggcgatg
    10921 atcgtcagcc tggttatcaa ggttgatatc cacatcaatg cgcctgttgt tatgactaag
    10981 agtgacaacc aactcgacat ctactctgcc agtggattgt cttgcgctgc gttgttgcag
    11041 attacgcggc accgccattt catagcggat aacagccctg ctctgccagg cctcatgaat
    11101 aatctcgcat tgcggtttcg cattcgccga agtaattacc cactcttctt ttgcgggtga
    11161 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaacaccc ggtcatagcg
    11221 cacaccgcta tctttatcta ccagttgtag cgttccatcc tcattgagcg caatttgcca
    11281 gaaagcattt tccagtatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    11341 attcgcttcg atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgtat
    11401 atcaaactcc ataaagggat cgtaattacc gtaatgaact atttgccgat cgattaggcc
    11461 tggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtctcg
    11521 tagattaaac tggctggcgc gcagccgcac agtggtgttg atcacttctt cccgcggcca
    11581 gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc tgcggcatgt tgtcggcgat
    11641 tttacgcata tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    11701 gacgatttcg cgatgaactt tgtcacgaca gcagcagccg atgctgtcgt gggcatgatt
    11761 ttttaagatc tctttccaca ttttctccag caagccgtgg tggtatccaa aacccaacgt
    11821 cctggccagc gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg
    11881 ggcaactttg atatccatgc gcgtggaacc gatggtgcga tgcacgcgca tatatttgcc
    11941 atcaataaat tcccctttca gggttgccag attatctcgc tgcgcttcga tcttctcaaa
    12001 tacctcttca aagcggctca tcacaaactt tcgttgagga taaatttcgc gcagtttctc
    12061 catcacttcg aagatatttt gctgcaatgg catctgatca tgcccattcg gcagcaaaat
    12121 ctctttggtt acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttgcgtaa
    12181 tccgttttcg tccgcaggta agtacttacc gatggcataa cccaacggca tcacctgcgc
    12241 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatccg taccgtggcg
    12301 ctcagaacat ccacgccaga acatggtgca ggtaatgcca aatccattgt agatatgcgg
    12361 cagttgcccg gacatgccaa aggaatcggg taaataacct attttcatcg gctcgccaaa
    12421 cgcgaggcag tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat
    12481 cgtggtatcg gtctgggtat accaggggcc gataatcaac ttgccggctt ctaccagttt
    12541 cttcaagcgg tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg
    12601 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc
    12661 catattattg accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca
    12721 gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
    12781 tatggcatta cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc
    12841 aggagaattg cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc
    12901 gccataacac cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccagcaccg
    12961 ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc
    13021 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta
    13081 atacctgcca gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac
    13141 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
    13201 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa
    13261 gcggctttat tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc
    13321 agcaacagcg cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
    13381 caggcgacag gttcccccac cacaaacagc atcagactgc ccgctcccaa tgtaccgagc
    13441 accgggtaga gataaaaagt caggaagcca ttgaatttac tgctaagacg caagtgattt
    13501 ttcatccagc gtatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg
    13561 gagccgatca tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct
    13621 gccagcgaat aggcggtata ggctgccagc accggtacca tcaaaattcc gagcagcccg
    13681 ccgcccagtt tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc
    13741 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tcccccccgc gacaatcaac
    13801 ggtacggcaa aagagatccc gctcaacagt gcctgtttca gctccgtttt gacgcttttc
    13861 accggttgcg tatcttgctg taaggtacgc gtctcatcgc cacgctcgag ggtaagcgat
    13921 tgttggatca acgcttctgc atggcgaatc ggctcggcaa caggcacaga aagcgcagga
    13981 atgccgttaa aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcagtt
    14041 gcactattga gttgatccgc cgttaaacgc ccttcaatac cgttagcacc ttgcttttca
    14101 acgtagacat tcacgccgag tttgcgtccg gctttttcca gatactccgc agccatgtag
    14161 gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga tagttggcgc gttggaaaaa
    14221 gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg agagcaattc atcaggcgtc
    14281 gtcgccgact gtatacgcgc ccgaatctca tcatccgcca ggcgcgtggt cagcgctgtc
    14341 agcagttgca tgtgcgtagt acccgcttca ttgaggggaa tcgccagcaa caccactaaa
    14401 tcaaccgctt ccgggccatc cacgccttcc cactgaagcg gctcgctgag tgtggcgacc
    14461 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcactt
    14521 aaggcggtcg ggccaaggct ttcacggcga tacacttctt tcagaaattg ctcagtactg
    14581 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt gaatggcctc ttcacggctg
    14641 gtaaagcgcg cattcagaca caacgcatcg cggtgggtca gagtcgtcag gttcatcatg
    14701 atcctcactt gatcgttttt atagtcgcgc cagtgtgccc gacaaaacaa taccattgcg
    14761 taatcaattt cacttgattg aaattatttg cattttattt gtattgttta caggatgagt
    14821 ttatcgggca taatggctaa attcgtatta atgagatgct aaataatggg acacaaaccc
    14881 ttataccgac agattgctga tcgcattcgt gagcaaattg cgcgtggcga gcttaaacct
    14941 ggcgatgcgt tacctaccga atcggcatta caaacagagt ttggcgtcag ccgggttacg
    15001 gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc tcgaaagcat tcagggcagc
    15061 gggacttacg tcaaagaaga gcgggtcaat tacgatattt ttcagttaac cagttttgat
    15121 gaaaaactgt cggaccgtca cgtcgataca cacagtgaag ttctgatatt tgaagtgatt
    15181 ccggctgacg attttcttca gcaacagcta caaatcaccg tgcaggatcg tgtttggcat
    15241 gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc tggaagaaac ctggatgccg
    15301 cttgcgttgt tcccggatct cacctggcag gtcatggaaa attcgaaata tcactttatt
    15361 gaagaagtga agaagatggt tatcgatcgt agcgaacagg aaatcattcc tctgatgcca
    15421 accgaagaga tgagccgtct gcttaatatc agccagacaa agccgattct tgaaaaagta
    15481 tcgcgtggat atttagttga tggtcgggtg tttgaatata gccgcaacgc ttttaatacc
    15541 gatgactata aatttaccct gatagcgcag cgaaaatcat cgcgataagc acaaaaaggc
    15601 catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa
    15661 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
    15721 gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
    15781 atccgcagtc cctttcccac cggcaatgat cgcacccgcg tggcccatac gtttgccttt
    15841 cggcgcagtc acaccagcga tgtaacccac aactggcttg gtaacatgct ctttgatgta
    15901 cgctgccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt
    15961 ctgcggatct ttttcgaaca tttcgagaat gtcgataaag ttagagccag ggatcgggtc
    16021 accgccgata ccgacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
    16081 ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg
    16141 acccggctgg ataccaattt tgcattcacc cggggtgata acgcctgggc agttcgggcc
    16201 gatcatacga acgcctgctt cgtccagttt cactttcacg gtcagcatat ccagcgtcgg
    16261 gatgccttca gtgatggtga taatcagttt gatgcctgca tcgatagctt ccagaatgga
    16321 gtctttgcag aacggtgctg gtacgtaaat aacagacgcg gaagcgccag tagcagcaac
    16381 agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtgccgc ctttacctgg
    16441 agttacgcca ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc
    16501 ctggctaccg gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
    16561 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
    16621 ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc
    16681 agacgtacca cgaccggtac gttaacaccc acttccgcta ccgcgccgat gataccgtca
    16741 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
    16801 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca
    16861 acgtccagga agttagccgg ttcgccgccg tgcagtttaa ctatgtccat cgtacccatc
    16921 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
    16981 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc
    17041 agatcaggct ggcggaacag ggcgttgcca tcagcgccca gtttgccgtc gaggcaaatc
    17101 agatcgccct gtttggtgat gaccagcggg ttgatttcga tcagcgccag gtcgcgctcc
    17161 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
    17221 agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
    17281 agcgcaactt tatggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
    17341 ccttcggtgg aggccataaa gaccacgcga cgggaactac ggtctacaac ggcaccgaga
    17401 tacagctctt tagcgatatc ggtcgccgct tcaaccagaa tctggttaac cggttggcca
    17461 ttggcatctg tttgatacgt taccagacgc ttgcccagcc agttttctgc aaaagcacgg
    17521 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
    17581 acctgacatt tcactaccca cggaccggca ccgatttttg aagcggcttc ttctgcttcg
    17641 cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaacagttgt
    17701 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
    17761 taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
    17821 tacacgtcca gcagcagacg cgtcggctct tccagtaact ctttgatcgt taccaggaag
    17881 cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc
    17941 ggcaggatct caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc
    18001 agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca
    18061 ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccatcacgg
    18121 cctttgactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca
    18181 tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa ccgccatgct gacgtcgaaa
    18241 tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt tcacttccgg gtaacgtttc
    18301 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
    18361 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
    18421 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
    18481 ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc
    18541 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    18601 acatcttcac gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga
    18661 ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    18721 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    18781 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt
    18841 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    18901 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt
    18961 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    19021 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    19081 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    19141 ggcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    19201 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    19261 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    19321 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat
    19381 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    19441 caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    19501 gatgacgcag cagggatttc ggcgacatca cgaccagcgg acgacgcatc ccgcgcagcg
    19561 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggtacgcaa acctgcatgt
    19621 tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    19681 gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc catttctgct
    19741 cgccagagga gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact
    19801 gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc ataaccatat tcaaacgcca
    19861 gcacggcttc ttctgacagt acggagtccc agacacggaa cgcgccctga ccgttatgga
    19921 tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    19981 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcatcaa
    20041 ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggctg
    20101 ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttctg
    20161 gtaccgtact gatacgttta gccagttcct gcaggcgctt catctcaact ttgttcgggt
    20221 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    20281 gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca
    20341 tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt
    20401 tgcgcggtgt cggatgtttt ttgattttct gatacatcag tggctgggtt gcgctcggct
    20461 cgtcggcttc gttgtggccg tgacggcggt agcataccag gtcgatgaag acatcacgtt
    20521 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    20581 ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg
    20641 tagaacgggc atccagcggg ttagaggtgg tgaaaccaac ctggttgttg ataacgatac
    20701 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca
    20761 cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgt
    20821 tcggctcatc aagtctgtcc agacgggcac gaacagagcc gataacgacc gggcttacaa
    20881 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    20941 agtcagacga gaagcccatg tggtatttca cgtcgcctgt gccgaggtgt tctttatgtt
    21001 taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    21061 tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg ggtgccgctg ttgccagcgt
    21121 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    21181 ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc ttcagcggcg gtcagttcgc
    21241 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    21301 ggatccagcg tttttcttcg gtgctggtga tgtgcatata ctcggcacca atcgggccgc
    21361 agtaggtttg cttgagggcg gaaataagct cgccgagttt catggtttct ttgccgctgg
    21421 caaatgaacc gacgttgaag gtttcctgga agtctgcttc ggtcagatcg tggaaagacg
    21481 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatctaga ttcgcatgct
    21541 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    21601 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    21661 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    21721 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    21781 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    21841 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    21901 accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta cgtagtggct
    21961 cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca
    22021 tttctttgtt acgcctgatg cgctgcgctt atcaggcctg cgggtaatga cttgtaggcc
    22081 ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcaacgctt gcgcgtctta
    22141 tcaggcctac agtttacgca ttacgttgca acaacatcga cttgatatgg ccgatggcgc
    22201 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    22261 atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    22321 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    22381 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    22441 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    22501 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    22561 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    22621 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    22681 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    22741 ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg
    22801 tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    22861 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    22921 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactca ggcagataca
    22981 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc
    23041 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    23101 tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    23161 gggcattttt cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat
    23221 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacggatcg
    23281 ccaccggatc ttcaccgtta cgattattgt tccaccggtt caggcgatcc agagacgctt
    23341 caacatcaga ctcgctggca tcgtgcagtg cgccctgctc ggcgatagac tcttgcagat
    23401 gcagacctgc cgcgcgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    23461 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagtccc ggaacaacca
    23521 catcttcgcc tttctcattc acagtcagcg cctgaccggt aactttggtc ggaataccgc
    23581 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag
    23641 cgaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca
    23701 ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac
    23761 ggatttcaat catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg
    23821 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    23881 ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca
    23941 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt
    24001 taatgtgggc gttagtggtg gactgataaa tacgccctgc tccgccagtc gccagcacgg
    24061 tagcgcgggc tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca
    24121 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg
    24181 tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag
    24241 cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    24301 gacgttgata gatacgacca tcatcgagac gcgagaacgg caggcccatg tgttcgagtt
    24361 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    24421 agtccgaccc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    24481 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    24541 tagagagcag ggcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg
    24601 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    24661 ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg
    24721 cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    24781 ccaggcatga atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    24841 ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac
    24901 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    24961 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    25021 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    25081 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    25141 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    25201 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccactg caggatgccc
    25261 actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    25321 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    25381 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    25441 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    25501 cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    25561 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct
    25621 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    25681 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    25741 acaaagttgt tacaaacatt accaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    25801 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    25861 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    25921 ttcgtcatca atggattctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    25981 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    26041 aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata
    26101 caaaagcaaa actcaacctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    26161 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    26221 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    26281 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    26341 tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    26401 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg
    26461 ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    26521 cgttctatca cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc
    26581 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    26641 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    26701 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    26761 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    26821 cttcgggggc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    26881 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    26941 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    27001 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    27061 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    27121 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    27181 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    27241 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agttacggta
    27301 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    27361 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    27421 cgggttaatt tttatatgcc taaacacaaa aaattgtaaa aataaaatcc actatcggac
    27481 attcatatat tctaaaaaaa atacatcggt ataaattata aacaaataga tgatttttaa
    27541 tcatctatct tcctctcagt tacatgaata aaccaccaaa tatagcgcac ccctataatt
    27601 cccaatactt tattcatcaa acatttaatt cataccatca attaattact tttacattta
    27661 ataaaattaa taatattttt aagaaaaact aaaaacattc gacaatcaaa taagatattc
    27721 ttgcaccgat atactttttt cttttctgtt aaaggactca gttatgttta aaggacaaaa
    27781 aacattggcc gcactggccg tatctctgct gttcacagca cctgtttatg cggctgatga
    27841 aggatccggt gaaattcact ttaaaggtga agttattgaa gcaccgtgtg aaatacatca
    27901 ggatgatatt gataaagagg ttgaactcgg tcaggtgacc accagccaca ttaatcagtc
    27961 acatcacagc gatgccgttg ctgtcgacct gctcttagtc aactgtgatc tggaaaactc
    28021 cagcaacggt tccggtggca agatttctaa agttgcagtc acctttgata gctcagcgaa
    28081 aaccaccggc gcagatccga ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg
    28141 cgtacgttta atgaataaag atcaaagcaa tatcgtttta ggcaccgcta ctccagatat
    28201 cgacctggct ccgacctcca gcgaacaaac gctgaatttc tttgcctgga tggaacaaat
    28261 tgatcaggct acacctgtaa cgccaggcgc agttacggcg aatgcaacct acgtgctgga
    28321 ttataaataa gtattacttt ggcggcgcta tcgccgcctt tcataaaata tttatcaggt
    28381 atggacaccg tgaatattta tcgactctct tttgtatcct gcctggtcgt ggcgatgcct
    28441 tgcgcattgg cggtcgaatt caatctgaat gttctcgata aatcaatgcg cgaccgcatt
    28501 gatatttcat tattaaagga aaaaggagtc attgctcccg gtgagtattt tgttagcgtt
    28561 gcggtaaata ataaccaaat cagtaacgag caaaagatta actggcacaa aaatgacgat
    28621 aaaaccattc cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa acctgaagtc
    28681 cgtcagtcgt taccattgat aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc
    28741 ttcaatttcg atcaagccaa tcagcaactg aatattagta ttccgcaagc ctggctggcg
    28801 tggcactcag aaaactggac cccaccctcc acatggaaag aaggtgtcgc cggtgtcctg
    28861 atggattaca acttgtttgc cagcagctac cgcccacagg atggcagcag cagcactaat
    28921 cttaacgcct atggaacagc cggaatcaac acgggggcat ggcgcttacg cagtgattat
    28981 cagttgaatc atactgatag cgatgataac catgaacagt caggcgaaat atcacgtacc
    29041 tatctttttc gcccgttacc gcaattaggt tcaaaattaa ccctcggcga aacggatttt
    29101 agtcccaata ttttcgacgg tttttcttat accggcgcgg cactggcaag tgacgatcga
    29161 atgttgccat gggaactacg cggctacgcc ccacaaatta gcggtattgc acagaccaat
    29221 gccacggtga cgatcagtca atcaggccgc gtcatttacc agaaaaaagt cccaccaggc
    29281 ccatttatca ttgacgacct taatcagtct gttcagggca cactggatgt caaagtgacg
    29341 gaagaagatg gtcgggtgaa caatttccag gtttcagcag catcgacgcc cttcctgact
    29401 cgtcagggac aggttcgcta taaactggcc gcgggtcagc cacggccctc catgtcacat
    29461 caaactgaaa atgaaacctt ttttagcaat gaagtttcct gggggatgct gtcaaacacc
    29521 tcgctgtaca gcggcctgct gctttctggt gatgactacc attctgccgc aatgggtatt
    29581 gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat
    29641 tttgatactc agcaggacga gcggggctta agctaccgtt ttaattacag caaacaagtg
    29701 gatgccacta acagcacgat ttccctcgcc gcttatcgtt tctccgatcg tcattttcac
    29761 agctacgcca actatctgga tcacaaatac aacgacagcg atgcgcagga cgaaaaacag
    29821 acgatcagct tatctgtggg ccaaccgatt accccactaa acctcaatct ttacgccaac
    29881 ctgctacatc aaacctggtg gaatgcagac gcctccacga ccgccaacat cacggcaggt
    29941 tttaatgttg atattggtga ctggagagat atctctattt ctacgtcatt caatacaacc
    30001 cattacgaag ataaagatcg cgacaaccag atttacctgt cgatttcgct ccccttcggt
    30061 aacggtggtc gggttggcta tgacatgcaa aacagtagcc acagcaccac acaccgcatg
    30121 tcgtggaacg atacgctgga tgaacgtaat agctggggca tgtctgccgg actgcaatcc
    30181 gaccgtcctg acaatggagc ccaggtgagc ggtaactatc agcacctgag ttcagcgggt
    30241 gagtgggata tttctggtac ctatgccgcc aatgattaca gttccgtcag cagcagctgg
    30301 agcggttctt tcaccgcaac ccaatatggt gcagcgtttc atcgccgcag ctccaccaat
    30361 gaaccacgcc tgatggtcag caccgatggc gtggcagata ttccggttca gggcaatctc
    30421 gactacacca accattttgg cattgcggtg gtgccgttga tttccagtta ccagccttcc
    30481 accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa cagttgcaga aaacgttatc
    30541 aaagaaacgt ggattgaagg cgcgataggt tacaaatcac tggcttcccg ttccggtaaa
    30601 gacgttaacg tcatcattcg caacgccagc ggtcagtttc ctcccctcgg agcggatatc
    30661 cgccaggatg acagcggcat tagcgtgggg atggttggcg aggaaggaca tgcctggtta
    30721 agcggagtcg ctgaaaatca aaagtttacc gtggtctggg gtgatagcca gcattgctcg
    30781 ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc tgattttacc ttgtcattaa
    30841 ttaaggaaat gaaaatgaca tttatgaaag gactacccct tttgttgttg gttgccagtc
    30901 tgtgcagcca cgctgcacta caacccgatc gcactcgtat tgtgtttaac gctaatgata
    30961 aagctaccag tctgcgggtg gataatcgta gcgataaact cccctacctc gcttattcct
    31021 ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct ggtcgccctg ccgccgatac
    31081 aacgtctgga accgaaagca acaacacagg tacggatagt aaagcaagcc tcgaccacaa
    31141 aattaccggg cgatcgcgaa acgctatttt tctacaatat gcgtgaaatt cctcccgccc
    31201 cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat tcagagtcgt atcaaggtgt
    31261 tctggcgacc ggctgcatta cgcaagaaag caggagaaaa ggtcgaactg cagttacagg
    31321 tcagccagca gggcaaccaa ctgacactga aaaatcctac cgcgtattat ctgaccattg
    31381 cctacctggg acgaaatgaa aaaggcgttc tccccggctt caaaaccgtg atggttgcgc
    31441 cttttagtac ggtcaacaca aacaccggaa attatagcgg cagccagttt tatctcggtt
    31501 atatggatga ttacggcgcg ttacgcatga cgacgctcaa ctgcagcgga caatgccatt
    31561 tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga gtgttgctcg tcatctgtct
    31621 gttatttctg tcgttgaagt ctgcgcttgc cctgaactgc tactttggtt catccggtgg
    31681 ctcggtagaa aaatcagaag cgattcaacc ttttgctgta ccaggcaatg ccaaactcgg
    31741 cgataagatc tgggaatctg acgatattaa aataagatct gggaatctga cgatattaaa
    31801 attcccgtct attgtgacaa caataccaac ggcaactttg aaagcgaaca tgtctacgta
    31861 tgggtaaacc cctacccggg ggtgcaggat cgttattatc aactcggagt aacttacaat
    31921 ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg acactaacca gtgtatcgac
    31981 agtaagaaca ttgacattta tacccctgat gggatggcag aacaaaattt gctcgggtga
    32041 tcccgctaat attcacatgt cgcgaacctt tctcgctcgc atgcggttat acgtcaaaat
    32101 acgagaaatg ccgccgcatg attatcaaag tacgcttagc gactatatcg tcgtgcaatt
    32161 tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa aacctgaaat atcatattac
    32221 tggtctggaa aacattcgcg tgctggattg cagcgtcaat ttttccattt caccggaaac
    32281 acagatgatt gattttggta aatttaattt gctggatatt cgtcgccaca caatgtcgaa
    32341 aacattcagt attaagacga caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt
    32401 cagttcctcc ttttataccg aagaaacgtt ggttgaagaa gataaagcat tgttgattgg
    32461 taatggtctg aagctacgtt tgctggatga aaacgcctcg ccttatacct tcaataaata
    32521 tgctgaatac gccgatttca ccagcgacat gttggtctac gaaaaaacct atacagctga
    32581 actttcgtcc attgcaggta cccccatcga ggctgggccc ttcgatacag tggtgctttt
    32641 taaaattaac tataactgat ttgccatgat gccctttgca gccggaaagg gcatctcttt
    32701 cagaaaattg ctgattttcc ccttttcctt gattgctgtt attttttcct agcccggctt
    32761 tctttatgct gtatcgatac accaaagaga aaataatgag ggagcgaagg atgccagttt
    32821 tgcagtgggg aatgttatgt gtgctatcac ttctcctttc tattggtttc ctcgcggtac
    32881 atctcccggc ggcgctatta cttgggccga tgatcgccgg gatcatcttc agtatgcgcg
    32941 gaattactct gcaacttccc cgctccgctt ttctcgccgc gcaggccatt cttggctgca
    33001 tgattgcgca aaacctcact ggttcgattc tcaccacgct ggccgccaac tggccgatcg
    33061 tgcttgctat tttactggtg acgctgcttt ccagcgccat cgtgggttgg ttattggtgc
    33121 gctatagctc actgcctgga aataccggtg cctggggttc ttctccaggc ggcgcagcgg
    33181 caatggtcgc tatggcgcaa gattacggcg cagatattcg cctggtggcg tttatgcaat
    33241 atctgcgagt gctgttcgtc gcgggggccg ctgttctggt cacccgcatg atgctgggtg
    33301 ataacgctga agcggttaac cagcaaatcg tctggttccc gccagtgagc attaatctcc
    33361 tgcttacgat tttgctcgca gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt
    33421 ccggcacgat gctcatcccg atgctggcgg gcgcagtgct ccagtctggt cagctcatca
    33481 ccatcgaact accggaatgg ctgctggcga tggcgtatat ggcaattggc tggcggattg
    33541 gtcttggttt cgataaacaa atcttactgc gggcactacg cccgctaccg caaatcctgc
    33601 tgtcgatttt tgctctgctg gctatttgtg caggtatggc gtggggactg acccggttta
    33661 tgcatattga ttttatgact gcctacctcg ccaccagtcc cggcgggctt gataccgtgg
    33721 cggtcatcac cgcagggagc aatgccgata tggcgcttat tatggcgatg caaaccctgc
    33781 gcctgttcag tattttgctg acggggccag ccattgcgcg gtttatttca acctatgcgc
    33841 cgaagcggtc ggcctagtgc tggcagcctg gacaccagta aaacgggcgc gaggacaacg
    33901 tggttttctc aatgatgccg ccacagcgtt cgcacagttc gccatcacga tgaaaaacct
    33961 taaagcgaaa cagcgcccca tgatgcttat tctcatccac ctgcccccgc gtagcgtaag
    34021 aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat
    34081 ctttcgcttt atgatttcca gtcaacccaa cctgccagag gatctccacc cgcaaataat
    34141 tgccaagccc agccagaaac gcctgatcga gcagtaatcc tgcaaactga cggttacgaa
    34201 agcgcggcga caataaccgt tcctttacca cctccggcgt cagattcgga tccagcacat
    34261 cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc aacatctcaa
    34321 tatccgaggc gctataaagc agaatggttt tgtcagccgt ttgcagtttt acccgcaata
    34381 ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg ccagacgccg taaagctgat
    34441 tatggctgta gagcgttaaa ccgttgggaa aatgagttaa caacgcctta ccacgcgttt
    34501 ccacatgggt aacgtgttga ccgataagtt gtgattgata agtttttaac tgcgggaagg
    34561 caaaccagac atcagttagt ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac
    34621 ggcggatctc cgggccttca ggcatggtga tatccttttt tcattcttta ttaagcgggg
    34681 ttatgctgcg acaacaatcc cctttttggc aaatgtagct cgtaatcggc gggcgaacgc
    34741 cagagcgtgc tcgccgtcgc catgcaggca gaccgtttgc gccgcgaccg ttgcccattc
    34801 gccggtgata cttttgactc tgccgtgttg caccatctcc agcgtttgcg ccaacgcctg
    34861 ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc accagcgagc cgtcagcctg
    34921 ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg
    34981 aatcagctcg cttcctgcca gcccaacgag aaccaatgcc ggatcgcaag cgtataccgc
    35041 tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg
    35101 cggtttgaca tgacgcatta cgccgccttg cgcacgggca atcgttgcca gcgcgccaat
    35161 ttgatacagc gtctgggcgt aaacggtttc cggcggcagc tgcatggcgc tgcgaccaaa
    35221 attttccctg tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt ttattgcttc
    35281 acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga aatccacaag caatattggc
    35341 agaggaaacc agcgttaata gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt
    35401 caggtcaatt ttcattgtgc agccgccacg ctaattgttc aaaataacgt tgctgatctt
    35461 gccgcgcttt cagtgcctct tccagtgaac actggacaaa atgaatcggc tgaccgagcg
    35521 gaatttgcgc cagatggtac atatcagcct caatgataca ggcaatacgt ggataaccac
    35581 cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc gttatgcggc acctgcacca
    35641 cgcccggtaa caaaccgtga gataacagtt cgcgatcggt gatgcgtttt aaaatttgcc
    35701 cctgtaaacg atagcccatt cggttacttt gcgagctaag ctgccagggc gaacgccaga
    35761 atgcatcctg cgaggcgcga tcgaactcat gatattccgg ccccggcaag gcgcgaatgc
    35821 ggttgcccca cagcagctgt ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc
    35881 caatcgggag tcagtcacca tccttcagta aacggccttc cagcccgcca atccccactt
    35941 tgagatcggt gctgcatgac cccattaccg gcggaacatc aataccaccc gcgaccgcca
    36001 gataactgcg catcccgtgc tgcgggcgtt taagcgttaa acgctggcct gctttcatcg
    36061 gcaatcgcca gccggtccag acggcgttat catccaaccg cgcttcgcaa cctgcacccg
    36121 tcagggcaaa ccacccatca gtttcgaact caactgttaa ctgaccgagc gtgatctcca
    36181 gcgcgggggc attggcgtca ttacccacca gcaggttagc aatgcgcagc gcaggcatat
    36241 ccagtgcgcc acagtggctg atacccgact ggcaaaaacc gtgacgaccg ccatcctgca
    36301 cagtggtgta catgcccgca cgaataatct tcagcatatt ccctccttct gcggtacaaa
    36361 tcgcacgctg tctcccggac gtaataagat gggttcgtca cgcgccggat caaacaggct
    36421 gagtgaggta tgaccaatca actgccagcc acccggcgtt gccagcggat aaacaccagt
    36481 ctgcggcccg ccgatcccga cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg
    36541 tggcgtgtgt agttgttccg gcaaactccc gagatacggg aagcccggtt gaaaacctaa
    36601 aaaccagacc acgtattcca cggaggagtg caattcaaca acctgttttt cgctcaaccc
    36661 gcaatacgcc gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat
    36721 ttcaataaag cgagactcag gttccagggc ctcgctctcc tcccaccagc gttgcaaacg
    36781 ctctatggca tccagcgcca gcgactcagg attacgcaaa atcaccgtga tattgttcat
    36841 gccgggaatg gcttcaacca cattcggcat atccaccaga cgctgcgcca gtcgccagat
    36901 ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc agcactaccg ccgtttcacc
    36961 tatcagataa caacgcgctc gttgcacttt cgatccctct gattattcgt tatgcaggat
    37021 taggaatatc aataaaggtc acatcaagat cggtattttc attcagccac tcgctcaatg
    37081 cgcgaatacc accgcgttca gtggcatggt gacctgcagc ataaaaatgc aatccctgct
    37141 cgcgggctga atgaatggtc tgttcagaaa cttcgccagt aataaaagca tccacgccaa
    37201 aacgcgcggc gctatcgata aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga
    37261 ccacctcagg tccggtatcg ccacaccata acggcttacg tcccagacgc gcttcaatcc
    37321 aggaggctag ttccattccc ggcacaggca tggtcagctc tccccacggc accaacggct
    37381 caatttcgcc catgaccgtg atccccagta acgccgccag ctgtgcgtta ttgcccagct
    37441 caggatgtgc gtcaagcggc aaatgccagc catacaggtt gatatcattc gccagcaacg
    37501 tttttaaacg gttacgcttc attccgcgaa tgaccggaga ctctcctttc cagaagtagc
    37561 catgatgcac aatgaccgca tccgcgccca gacgcactgc ctcatcgagc aaagcctggc
    37621 tggcggtgac accggtaaca attttttgca ccgtctcttt gccttccacc tgcaaaccat
    37681 tcggcgcgta atcactaatc gccgcgctgt tcagtttttc gttaatcagt tgttccagtt
    37741 cggtgttttt catcttcgcg ccttccgtat tctgatagct acaccgttaa catagcgtta
    37801 catcgcctgt tcgtcgatag ccaatctttt gacatattaa gaaacaaacc cgacgccaaa
    37861 agacgcaaat tgtttatatc atcacgcttc agctaatgta tatttatgca atcagacagc
    37921 atattgcata aataatcaga tctgtatcaa agttttaact ttaaggaaac acaagcacgc
    37981 cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt attatgaata aacacgcatc
    38041 acaaccgcgc gctatttact atgtcgttgc gctgcaaatc tgggaatatt ttagctttta
    38101 cggcatgcgt gccctgctga ttctctatct caccaatcaa ctaaaataca acgatactca
    38161 cgcctacgag ttatttagcg cctactgttc gctggtgtat gtcacgccaa tcctcggtgg
    38221 ctttttggcg gataaagttc tcggcaatcg catggcggtg atgctggggg cgttgttgat
    38281 ggcgatcggt catgtggtgc tgggtgccag tgagatccat ccgtcattcc tctatctgtc
    38341 cctggcgatt atcgtctgcg gctatggcct gtttaaatct aacgtaagct gtctgctcgg
    38401 cgagctgtat gagccaaccg atccgcgtcg tgatggcggt ttctcgctga tgtatgcggc
    38461 gggtaacgtg gggtctatta tcgcacctat cgcctgtggt tacgcccagg aagagtacag
    38521 ctgggcgatg ggctttggcc tggcggcggt tggaatgatc gcgggtctgg tcattttctt
    38581 atgtggcaat cgtcatttca ctcatacccg cggcgttaac aaaaaagtac tgcgtgcgac
    38641 aaactttctc ctgccgaact ggggatggct gctggttctg ctggtggcaa cgcctgcact
    38701 gattaccgtg ctgttctgga aagagtggtc ggtatacgcc ttaattgtcg cgactattat
    38761 tggcctgggt gtactggcaa aaatttatcg caaagcagaa aaccagaaac agcggaagga
    38821 gctggggctg attgtgacgc tcaccttctt cagtatgttg ttctgggcct tcgcacaaca
    38881 gggcggtagc tcgattagcc tttatatcga ccgcttcgtt aaccgcgata tgtttggtta
    38941 taccgttccg accgcgatgt tccagtcgat taatgccttc gcagttatgc tgtgcggtgt
    39001 gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg
    39061 aaaatttgct cttggccttg gcctgatgag cgccggattc tgcattctga ccttaagcgc
    39121 ccgctggtcc gcaatgtatg gtcactcttc tctgccactg atggtgttag gcctcgcggt
    39181 gatgggcttt gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga
    39241 aattccgggc gtgaccggcg tattaaccgg catctacatg ctgctttctg gcgcgatcgc
    39301 gaactatctg gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg atgcttccgg
    39361 ggcgatcaac tactccatca atgcatatat tgaagtattt gatcaaatta cctggggcgc
    39421 actggcgtgt gtaggactgg tactgatgat ttggctgtat caggcgctga aattcagaaa
    39481 ccgcgcgctg gcgctggagt cataataggc cagcaccgat gcctgatgcg acgcttgacg
    39541 cgtcttatca ggcctacgta tttcctgcaa tttattgaat ttgcacaaat ttgtaggctg
    39601 gattaggcgt tcacgccgca tccggcatct gggctcgatt gcctgacgcg tctgttattt
    39661 ccccttccgc gccgcctcat acgctgccaa cgtttgtact ctcgcctctt tgtgctcgac
    39721 tatcggttgc ggataatcca gcttcacacc tgctttctgc gcccacttcc acggctcatg
    39781 caccgctttc cctggtacat cgcgcagctc cggtaaccat cgacgaataa actcgccctc
    39841 acggtcaaat ttctcgccct gagttatcgg attgaaaata cgaaaatacg gcgcagcatc
    39901 agtacccgta gaagccgccc actgccagcc accgttatta gctgccaaat caccatcaat
    39961 cagctgcgac atgaaatatc gctcgccttc gcgccagtcg atcaacaaat ctttcaccag
    40021 aaaactggct gtaatcatcc gtagccggtt atgcatccag ccagtgctgt taagctgacg
    40081 catagcggca tcaataatcg ggtatcccgt tttgcctttc tgccaggcct gtaaatgtgc
    40141 gggattgctt tgccactgca cacgatccgt ccaggcaata aatggacgat gtttacacaa
    40201 cgaggggtaa tacgtcatca gatgacggta gaactcgcgc cagatcagct cactaagcca
    40261 gacactaccg gccccaccgt ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca
    40321 ctggcgaggc gataacccgc ccgttgccaa gctggcggac aaacggctgg tgccttccac
    40381 tgccggaaaa tctcgttgtt gctcatactc tccagcaccg ttctcgcaaa actggcgtaa
    40441 ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag actgacgagg
    40501 ataattcagc gtaatggatg gcgcgggctt tatcgatccg ctactacgaa cttttggtgc
    40561 agcgacgcac tccggcatcc cttcccgcag ccgtttcagc caggcattct taaaaggcgt
    40621 aaagactttg tacatctcgt gattgccggt catcaccgcc cccggcggca ggatcacgct
    40681 gtcatcaaat ccttcacaca ccacgttacg cagcgctctt tcaacgtcca catcccgcgc
    40741 tcgctcattc acctcatact gatagttata aaaaagatga gtaacgctgt tttccgcgca
    40801 cacctgttta actatttcga cactggcgac aaagtcatcc acttcacgga acaataaagg
    40861 aatacctttt tccgccagcg cgatttgtag cccattcagt tgagcattga taagttcagc
    40921 ctgacgcggc gacatgttat gcgtctccca cttgcgtggt gtagcgatat acagcgccag
    40981 cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg agattatcgt gcagacgtaa
    41041 atcctggcga aaccaggcca gatgggtagt cataaaactc ctgaatggcg caagttaatc
    41101 acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc
    41161 atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcgccacg gcgataactg
    41221 tcgataagcg cagccagctg ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc
    41281 tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga aagcaaaaca
    41341 attacgcgtt ggcggtactg gttgtaaaaa tcatccagat tttgccactg atgaatatca
    41401 gccacaaacg ggccaagttt gcggtactcg ggctgagaat gggccagaaa gacgagttta
    41461 tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt cagtcgatgt
    41521 aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg tagattcatt
    41581 actgctcctg aacggtatac aggagtaagt gtaggcgttg gtaaatgatg tttgtgaagt
    41641 taatccgcta gcagggcaag attacgaaag cgcgctcccc gcaagcaact aatcaaggta
    41701 gttaatcatg gctgaacacg atattacgcc agaagtcact atttccaaaa cacagcgccg
    41761 ctataaagtc ggctatgtca gagcccgcca ccaggaccgc aaaaccggta tagcccggta
    41821 ttattcacaa catcccagtc tgcaccttaa gggcgactgg ctgaaagagg ccggatttga
    41881 taccggacgg ggtgtcacgg tgaagatttc ggaggggggc ctgaccatca tcgctgacag
    41941 tgacgaggtg caggaactgc gggatgggat gtttagtgtg ctgaacgaaa gttaaatatt
    42001 aaaaaagctg ggaagattta aaaccttcct agctttttat tctgtcttat tataatggat
    42061 actcatcatc ttccgatgaa tttataaatt gctccaagtt tacatgaaga gtacttaacc
    42121 tagtttttaa ctcattaagt gaagcgataa tttcatattt tccaacataa tcatatgatg
    42181 gatcgtattc attatctatg tcagaagcta aaaatgatga tattcgtagt ttaaatttag
    42241 cttcatcttt attacattca ttggcataag gtgaatgccc tatcatagca tattcatcta
    42301 atctaaaagg ataagatggt tttaattcat cgatcaatga aatttcaatc catgttctat
    42361 atccaggcat taatggacta atttctttga gcaaaacctt tccacttaat aatgtattga
    42421 gattgttcat tattatgtct cccacgggaa aaatatatgt ctaacacttc caccatgttg
    42481 atgaacttgt aaatgtggta aatcaccatc aggatttcct ggtccatgac ctgacactct
    42541 atatgaaggt tttgttacag aaggatcttt tatatcatcc caatgatatg aacctgcttt
    42601 tgttttattt tcagtaagat attccattaa gttcttagtg ctggggcctg actcacctgt
    42661 catattttta taattatgac ctaaatatct atccataaac aattgttcag cgtcacttct
    42721 agacctaaca gtaactttgg tttcactact atctaataaa cgattcgctt tttggttact
    42781 aacagcacat ggagttaatc caagtggatc agcatatttt agaggattag gtgcataact
    42841 gtagagattt aaaccgcctc tcaaccctat cgggtcagtt accatgaacc gcccgacatc
    42901 cggatcatag tacctgtgca gattgtagtg cagccccgtt tccctgtcga ggtactgtcc
    42961 ctggtagcgc aggttctgct ccaccccgcc gtggtccttc tcctgtatcg tgttacccca
    43021 gacctgatac cccgtctccc acacgatttt cccgtcgctg tccgtcatct cttccggcgc
    43081 gccgttcaca tccgtgtgaa aatacagtat ccggttcggg ccctctttgc ccgccttatc
    43141 cacccgcgcc agcggttcat aactcccctg gtccctgtag atgtacaggc tgcctgttcc
    43201 ctccggcctg ctctcctgcg ccagccgcat tccgtcccat aaaaattttg tccggttgta
    43261 cggcttaccg tcagggctga tgtgctgttt ttccgtccgc ctccccagtg catcgtacac
    43321 ataccggtat gtcccccgcg ctgtcgtcac ttccaccatc cggtgttccg catcataacg
    43381 gtaactctgc gttccgctcc ggccctcctt ttccaccgca cggccaaatt cgtcataacg
    43441 gtagtgcaca ccacggtaag acatcagccg gttgaacggc accacgctgt tttctgccgt
    43501 ggccctttcc cccgcgcgcc tccccagcag gtttcccgcc gcgtccacct ccgtttccgg
    43561 ctggtgccac accgggtgcc actgcgcctg ctccacccgc cccagcgggt cgtgcgtctc
    43621 cgtgatatgc ccctgcaggt catacacata gcgccatttg ccgccctgag ggtcggtcat
    43681 ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc gtcacctgtc cggcttcgtc
    43741 ctggtacgcc gtcacctgat tctgcgcatc ccactggtga taacgtgtgc gccccagtcc
    43801 gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc agccggtact gttcaccgct
    43861 gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc ggaccttcat accagcccca
    43921 ctggtaataa tacccttcgc cgcccggctg gcggtgcagc gtcatgcagt ggttttcgtc
    43981 gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac acgccgttca ggtggccggc
    44041 ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc tcagggaaat gctgataaat
    44101 ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac agacggatgc acggacgctg
    44161 ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg tagtacagat tcagacagtt
    44221 gctgttacgg tcgcccagca ttttcagccg ctggcgctgc gggtggtgcg ggtcagcctc
    44281 aaacagaaaa cactgcccgt catcgctgct gataagccag tgcccctgct caccacgcct
    44341 taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc ggcagtacgg cttccatatc
    44401 caagcgtcgc cccatgcccg acacaaaggt catgcagttt tcgtctgcgc tacccggtgt
    44461 gcacacgagg ctgacttcat acattacact ccagccctga ccaaacattc cttccgtacg
    44521 ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga aagtggcccg gcagactgaa
    44581 atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg gcggcattga ccggatgcgg
    44641 aagggcactg gtggccttct gcgtcagcat gctcatcccg acactggcag caaaacaggc
    44701 gattttagaa agcattttgc ccgggcgaag aaacatcagc gccacggttg ccaccagggt
    44761 tatctgcgac ctgccgcttc tgatatccct gaccaccagt aacggaccgc caatgcgcac
    44821 atccggtgac acaaacgccc cgttccccgg ctcattcacc accctggcct cgcaggtaca
    44881 gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc acatcccgcg cgccttccgc
    44941 gatatattcc gctgcgggcg gctcgtcatc gcctttgaac agattactga tgctgccaat
    45001 aaccgcattc actccctgaa aggcggtttc cagcataccg cccggattca gcaggttaag
    45061 ggccgttccc agggggtcat ccagaaaggc ggcttttttt tgtgcgagaa actgcgtcag
    45121 tgaggaagga tgtttatggc agtcgatttt atcgtcaggg gtcggggtgg atcccggggc
    45181 cgcactggcc acagtgggct gccatagttc cgacacaaaa tgtgtcacgc catccaccat
    45241 ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga gcccgggaca tcagcacagg
    45301 ccctgtcacc gggttaaccg ccagggctat ggcccccgcc atttccagat attccccggc
    45361 cacatcccgg aacgttctgg catcctcctg acgctgcgct tcttcctgtt ctgccttcgc
    45421 ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca gtcgccgctg cccgcgcggc
    45481 aggtttgctg ttgatccatg tgtcaccaga gccggtgagg atgtaacctt caatctgagg
    45541 cgggaaaagc atatttgcga gggctgaaca ggcttcgctg accttttctg tcacgccggt
    45601 gtacaccatc gccacacccg ccacaaaacc ggtcagcacg acggccgtgc accccatcgt
    45661 gatccccact gacgtcgcca gtaacgaggt tgtgccgaca atgagggcat caataacgac
    45721 atttgcggcc acctccagca ccgcccccag cacatctgcc ataaacgacg tatgcagcag
    45781 ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg tcccgttccc tgtatgatta
    45841 attcgtcctg acttcctgcc gcatcctgaa gcgcagattc tttttaattt cagcccagcg
    45901 cgtcatatct gccggggtca gcggtgtggt tctggcgtag gtgaaggcca tcatgacccg
    45961 ctcacccggc aatacaaatg ccacctgaca ctggtacacc tgctcgttac cccggagaaa
    46021 ctggctctgt atttccaaac cgcctcgttg agataatatt ccgtcataat gtaaatagtc
    46081 cgttttaata aaggaattat ttattgacag gttctgtttt tttaatcatg aatgtaatac
    46141 ccatcaccgc tttacagcac actttatctt tccagtcata atcactaata atattaagca
    46201 attagcaata tttacaacga ccagagcgga tacatagtta tgtatttaac aatcccaata
    46261 tattcactat gcaaataaaa ccactagtaa cagacacgct accgcctcca tataaaaatg
    46321 gacaccttat aaaattgtat cttgcataga aaaagctgaa atatcggatg cgttttaatt
    46381 atcaactata aatctataag ctttttatta acaac
//
LOCUS       DAJTMX010000016        45511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTMX010000016 DAJTMX010000000
VERSION     DAJTMX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.016"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..428)
                     /locus_tag="OO145_000912"
     CDS             complement(<1..428)
                     /locus_tag="OO145_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5466119.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATL"
     gene            complement(416..799)
                     /locus_tag="OO145_000913"
     CDS             complement(416..799)
                     /locus_tag="OO145_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5466120.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(815..1123)
                     /locus_tag="OO145_000914"
     CDS             complement(815..1123)
                     /locus_tag="OO145_000914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466121.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(1124..1702)
                     /locus_tag="OO145_000915"
     CDS             complement(1124..1702)
                     /locus_tag="OO145_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5466122.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(1725..2156)
                     /locus_tag="OO145_000916"
                     /pseudo
     CDS             complement(1725..2156)
                     /locus_tag="OO145_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            2528..3547
                     /gene="epd"
                     /locus_tag="OO145_000917"
     CDS             2528..3547
                     /gene="epd"
                     /locus_tag="OO145_000917"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5466123.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            3597..4760
                     /gene="pgk"
                     /locus_tag="OO145_000918"
     CDS             3597..4760
                     /gene="pgk"
                     /locus_tag="OO145_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5466124.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            4975..6054
                     /gene="fbaA"
                     /locus_tag="OO145_000919"
     CDS             4975..6054
                     /gene="fbaA"
                     /locus_tag="OO145_000919"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5466125.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            6245..7105
                     /gene="mscS"
                     /locus_tag="OO145_000920"
     CDS             6245..7105
                     /gene="mscS"
                     /locus_tag="OO145_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5466126.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            7244..7879
                     /gene="argO"
                     /locus_tag="OO145_000921"
     CDS             7244..7879
                     /gene="argO"
                     /locus_tag="OO145_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5466127.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            7972..8712
                     /locus_tag="OO145_000922"
     CDS             7972..8712
                     /locus_tag="OO145_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5466128.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            8879..9829
                     /locus_tag="OO145_000923"
     CDS             8879..9829
                     /locus_tag="OO145_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5466129.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(9771..11249)
                     /gene="scpC"
                     /locus_tag="OO145_000924"
                     /pseudo
     CDS             complement(9771..11249)
                     /gene="scpC"
                     /locus_tag="OO145_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(11273..12058)
                     /gene="scpB"
                     /locus_tag="OO145_000925"
     CDS             complement(11273..12058)
                     /gene="scpB"
                     /locus_tag="OO145_000925"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5466130.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(12069..13064)
                     /gene="meaB"
                     /locus_tag="OO145_000926"
     CDS             complement(12069..13064)
                     /gene="meaB"
                     /locus_tag="OO145_000926"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5466131.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(13057..15201)
                     /gene="scpA"
                     /locus_tag="OO145_000927"
     CDS             complement(13057..15201)
                     /gene="scpA"
                     /locus_tag="OO145_000927"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5466132.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(15405..16298)
                     /gene="argP"
                     /locus_tag="OO145_000928"
     CDS             complement(15405..16298)
                     /gene="argP"
                     /locus_tag="OO145_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5466133.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            16681..17340
                     /gene="rpiA"
                     /locus_tag="OO145_000929"
     CDS             16681..17340
                     /gene="rpiA"
                     /locus_tag="OO145_000929"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5466134.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            17596..18828
                     /gene="serA"
                     /locus_tag="OO145_000930"
     CDS             17596..18828
                     /gene="serA"
                     /locus_tag="OO145_000930"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5466135.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            19057..19116
                     /gene="ibsC"
                     /locus_tag="OO145_000931"
     CDS             19057..19116
                     /gene="ibsC"
                     /locus_tag="OO145_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5466136.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            19449..19508
                     /locus_tag="OO145_000932"
     CDS             19449..19508
                     /locus_tag="OO145_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5466137.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(19609..20211)
                     /locus_tag="OO145_000933"
     CDS             complement(19609..20211)
                     /locus_tag="OO145_000933"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5466138.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(20232..20415)
                     /gene="ssrS"
                     /locus_tag="OO145_000934"
     ncRNA           complement(20232..20415)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO145_000934"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(20457..20786)
                     /gene="zapA"
                     /locus_tag="OO145_000935"
     CDS             complement(20457..20786)
                     /gene="zapA"
                     /locus_tag="OO145_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5466139.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            20954..21532
                     /gene="ygfB"
                     /locus_tag="OO145_000936"
     CDS             20954..21532
                     /gene="ygfB"
                     /locus_tag="OO145_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5466140.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            21558..22883
                     /gene="pepP"
                     /locus_tag="OO145_000937"
     CDS             21558..22883
                     /gene="pepP"
                     /locus_tag="OO145_000937"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5466141.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            22880..24058
                     /gene="ubiH"
                     /locus_tag="OO145_000938"
                     /gene_synonym="visB"
     CDS             22880..24058
                     /gene="ubiH"
                     /locus_tag="OO145_000938"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5466142.1"
                     /translation="MSVIIVGGGMVGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            24081..25283
                     /gene="ubiI"
                     /locus_tag="OO145_000939"
                     /gene_synonym="visC"
     CDS             24081..25283
                     /gene="ubiI"
                     /locus_tag="OO145_000939"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5466143.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(25357..25440)
                     /locus_tag="OO145_000940"
     CDS             complement(25357..25440)
                     /locus_tag="OO145_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466144.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            25731..26825
                     /gene="gcvT"
                     /locus_tag="OO145_000941"
     CDS             25731..26825
                     /gene="gcvT"
                     /locus_tag="OO145_000941"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5466145.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            26849..27238
                     /gene="gcvH"
                     /locus_tag="OO145_000942"
     CDS             26849..27238
                     /gene="gcvH"
                     /locus_tag="OO145_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5466146.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            27357..30230
                     /gene="gcvP"
                     /locus_tag="OO145_000943"
     CDS             27357..30230
                     /gene="gcvP"
                     /locus_tag="OO145_000943"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5466147.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            30497..31240
                     /locus_tag="OO145_000944"
     CDS             30497..31240
                     /locus_tag="OO145_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5466148.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(31297..32730)
                     /gene="bglA"
                     /locus_tag="OO145_000945"
     CDS             complement(31297..32730)
                     /gene="bglA"
                     /locus_tag="OO145_000945"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5466149.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            32775..33085
                     /locus_tag="OO145_000946"
                     /pseudo
     CDS             32775..33085
                     /locus_tag="OO145_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            33249..33908
                     /locus_tag="OO145_000947"
     CDS             33249..33908
                     /locus_tag="OO145_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5466150.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(34104..35084)
                     /gene="ygfZ"
                     /locus_tag="OO145_000948"
     CDS             complement(34104..35084)
                     /gene="ygfZ"
                     /locus_tag="OO145_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5466151.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            35327..35593
                     /gene="sdhE"
                     /locus_tag="OO145_000949"
     CDS             35327..35593
                     /gene="sdhE"
                     /locus_tag="OO145_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5466152.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            35574..35981
                     /locus_tag="OO145_000950"
     CDS             35574..35981
                     /locus_tag="OO145_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5466153.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(36021..36542)
                     /gene="fldB"
                     /locus_tag="OO145_000951"
     CDS             complement(36021..36542)
                     /gene="fldB"
                     /locus_tag="OO145_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5466154.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            36654..37550
                     /gene="xerD"
                     /locus_tag="OO145_000952"
     CDS             36654..37550
                     /gene="xerD"
                     /locus_tag="OO145_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5466155.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            37575..38285
                     /gene="dsbC"
                     /locus_tag="OO145_000953"
     CDS             37575..38285
                     /gene="dsbC"
                     /locus_tag="OO145_000953"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5466156.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            38291..40024
                     /gene="recJ"
                     /locus_tag="OO145_000954"
     CDS             38291..40024
                     /gene="recJ"
                     /locus_tag="OO145_000954"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5466157.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            40115..41213
                     /gene="prfB"
                     /locus_tag="OO145_000955"
     CDS             join(40115..40189,40191..41213)
                     /gene="prfB"
                     /locus_tag="OO145_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5466158.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            41223..42740
                     /gene="lysS"
                     /locus_tag="OO145_000956"
     CDS             41223..42740
                     /gene="lysS"
                     /locus_tag="OO145_000956"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5466159.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(42783..43331)
                     /gene="idi"
                     /locus_tag="OO145_000957"
     CDS             complement(42783..43331)
                     /gene="idi"
                     /locus_tag="OO145_000957"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5466160.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            43386..43457
                     /locus_tag="OO145_000958"
     CDS             43386..43457
                     /locus_tag="OO145_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466161.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(43454..43579)
                     /locus_tag="OO145_000959"
     CDS             complement(43454..43579)
                     /locus_tag="OO145_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466162.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(43581..45029)
                     /gene="uacT"
                     /locus_tag="OO145_000960"
     CDS             complement(43581..45029)
                     /gene="uacT"
                     /locus_tag="OO145_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5466163.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11042 a        11425 c        12145 g        10899 t
ORIGIN      
        1 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
       61 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
      121 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
      181 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
      241 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
      301 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
      361 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
      421 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
      481 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
      541 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
      601 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
      661 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
      721 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
      781 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
      841 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
      901 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
      961 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     1021 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     1081 aggccgctaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     1141 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     1201 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     1261 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     1321 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     1381 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     1441 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     1501 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     1561 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     1621 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     1681 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     1741 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     1801 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     1861 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     1921 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     1981 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     2041 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     2101 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     2161 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     2221 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     2281 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     2341 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     2401 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     2461 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     2521 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     2581 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     2641 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     2701 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     2761 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     2821 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     2881 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     2941 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     3001 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     3061 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     3121 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     3181 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     3241 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     3301 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     3361 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     3421 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     3481 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     3541 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     3601 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     3661 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     3721 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     3781 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     3841 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     3901 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     3961 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     4021 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     4081 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     4141 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     4201 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     4261 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     4321 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     4381 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     4441 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     4501 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     4561 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     4621 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     4681 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     4741 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     4801 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     4861 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
     4921 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     4981 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     5041 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     5101 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     5161 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     5221 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     5281 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     5341 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     5401 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     5461 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     5521 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     5581 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
     5641 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
     5701 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
     5761 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
     5821 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
     5881 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
     5941 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
     6001 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
     6061 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
     6121 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
     6181 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
     6241 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
     6301 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
     6361 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
     6421 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
     6481 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
     6541 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
     6601 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
     6661 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
     6721 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
     6781 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
     6841 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
     6901 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
     6961 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
     7021 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
     7081 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
     7141 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
     7201 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
     7261 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
     7321 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
     7381 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
     7441 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
     7501 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
     7561 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
     7621 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
     7681 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
     7741 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
     7801 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
     7861 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
     7921 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
     7981 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
     8041 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
     8101 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
     8161 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
     8221 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
     8281 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
     8341 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
     8401 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
     8461 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
     8521 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
     8581 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
     8641 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
     8701 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
     8761 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
     8821 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
     8881 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
     8941 tgccagggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
     9001 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
     9061 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
     9121 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
     9181 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
     9241 gaccgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
     9301 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
     9361 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
     9421 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
     9481 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
     9541 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
     9601 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
     9661 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
     9721 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
     9781 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
     9841 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
     9901 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
     9961 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    10021 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    10081 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    10141 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    10201 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    10261 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    10321 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    10381 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    10441 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    10501 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    10561 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    10621 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    10681 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    10741 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    10801 taggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    10861 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    10921 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    10981 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    11041 tcagcggcgg cgttgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    11101 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    11161 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    11221 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    11281 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    11341 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    11401 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    11461 cagagatgtg gtgcgccatt tgtaaggtga aatcttccag ttcttccact tccacaacat
    11521 ggttgaggat gccgacagcc agcgcgcgct gggcggtgat tggcgaagcg gtaaaaatca
    11581 gctctttgac aatgtggaag cccgcgtcgc gggtcaggtt gtgaatgccg accaggttat
    11641 acgggacgcc gaggtttaca ggcgtcattg agaaggttga ggtactggcg gcgatgatca
    11701 gatcggaact catgatcatt tcaaatgcgc caccccaaac actaccttcc accatcgaaa
    11761 tgatcggttt cgggaatttt tggatcatgc gggtgatttg acgcaatgga tcatcatagg
    11821 agagcggatc gcgaccgcca gacggcagtt cgtgaatatc gtgacctgcg gagaagactt
    11881 tggatccact tggcgcccgc aaaatgatac agcgaatttc cggccggttg agatcgctta
    11941 acgcctgcat aagatcatca ataaagactt tacttaaggc attaagtttt cgtccatagt
    12001 taaactcaat gaccgccact ttgttgatag tggcaacgtt cacatactga taagacataa
    12061 aaattccttt aatcaaaata ttgcgtctgg ataaattcac tgagctgccg caggccggtg
    12121 cgcggtgaga gcgtattgtt tttgaccgct aaaagcgtct ggcggtaata gcgatcgaaa
    12181 tcttcattcg cgaacaggtg attcagtact tcttcttcgg tctgcttacg cagccattcc
    12241 accgattgtt gttgccgcac ttgttgtaaa cgaccactgg cagttagcgc ggttttgaag
    12301 tcgatgatgg cgtgccagat ctcatcgatt ccacgttttt ccagtgcgct acaagtcaga
    12361 ccccgtggct gccattcgtc gtatttacgt cgcagaatat gcagggcact ctcgtacata
    12421 tgccgggcaa tggcgacatt ggtatggtta tcgccatcgt ctttgttgat aacgagcaga
    12481 tcagccactt ccatcagccc ttttttaatg ccctgcagat catcgccgcc accggcaatt
    12541 tgcaacgaga taaaacagtc caccatgcgg gcgacttccg tttccgactg cgcgatgcca
    12601 accgtttcga caatcactac gtcataaccc gccgcttcgc ataacagcat taattcccgt
    12661 gctcgctgac tggcaccgcc cagatgaccg gaggagggta ccgggcgaat aaacgccgcc
    12721 tcggcacgcg ccaggtcatt catgcgggtt ttatccccga gaatgctacc gccagtgacc
    12781 gggctgctgg gatcgaccgc aataacagca acctttaatc cctcacgaat caacaacatg
    12841 ccaaaggctt caagaaaggt acttttcccc gcgccgggtg tgccggtaac gcccagtcgc
    12901 agggcgttac cacagaacgg cataatagcg tcaagcagct gtgtacttag cgcctgatga
    12961 cgtgggtgac ggctttccac cagcgtcatg gcttgggcga gcgtggcacg ctcaccctga
    13021 cgtaagcggc gaatactttc tgccagcgtg gcttcattaa tcatgatgct ggcttatcag
    13081 attcagtacg tcgcgcacac tgtcgagcat aggtgtacct ggaccataaa tcgccgccac
    13141 gccgcgctct tgcaggaagg cgtaatcctg cggcggaatg acgccacccg cgaccacgca
    13201 gatatcttcg cgtccccatt ttttcagcgc ttcgaccagt tccgggatca gcgttttatg
    13261 accggcagcc agtgaggatg cgcccactac gtgaacgtcg ttttctacgg ctaggcgggc
    13321 gatctcttca ggtgtagaga acatcgggct taaatctacg tcgaaaccga gatcggaata
    13381 ggcgctggcg atcactttcg cgccgcgatc gtgtccatcc tggcccatct tagcgatcag
    13441 aatgcgcggg cgacgaccat tgtcggcaag gaactgctcc gtttgcgcaa caatggcatc
    13501 gaactcggag gccgatttct cagactgatg atagctttgc gcaatcacgc cggtaacaca
    13561 ctggcttggc accagataac ggtcgaaagc gacttccagc gcatcggaaa tttcacccag
    13621 ggtggcgcga acgcgagcgg cattaacagc ggcagccagc aggttttcgt tatgctgtgc
    13681 ggcgtgagtc agggcgttca acgcggcggt tacggcggca tcatcacggg tggcgcgaat
    13741 gcgttccagc gaagcaattt gctcgttacg caccatcacg ttgtcgatct caagtacatc
    13801 ggtttcgtct tcgtgatcca gtttgtactt gttgacacca acgatgacac gcttgccctg
    13861 gtcgatcagc gactgttcgc gcgctgaggc ctcttcgatc attcgttttg gcagacctgc
    13921 ttcgatcgct ttcgccatgc caccggcttc gtcgatctgt tggataatag ctctggcttg
    13981 tttgacgatt tgatcggtca gcgactcaat gtaataggat ccggccagtg gatcgacggt
    14041 gtggcagagt tctgattctt cctggatgat gatctgggtg ttgcgggcaa tgcgtgctga
    14101 gaaatcggta ggcaaaccaa gcgcttcgtc aaaggcgttg gtatgcagtg actgagtacc
    14161 gcccagcgtc gcagccagtg cttcaatggt ggtgcggata acgttgttat acggatcctg
    14221 ttcagtcagg ctccagcctg aggtctggca gtgggtacgc agcgccagtg atttcggatc
    14281 ctgtgcgcca aatccactga ccgcttcgct ccataaataa cgtgccgcac gcaacatggc
    14341 gacgttcata aacagatcca tgccgatacc gaagaataac gacaggcgag gagcgaagtc
    14401 atcaattttc agtccggcag agattgctgc tttgatgtac tcaatcccat cggcgagcgt
    14461 aaatgctacc tgctgcacgc agttggcacc cgcttccccc atgtggtaac cgctgatact
    14521 gatggtatta aatcgcggca tgttgccgga acaccaggcg atgatgtcgg cgataatgcg
    14581 cattgacggt tttggtgggt aaatataggt gttgcggcag aggtactctt tgagaatatc
    14641 gttttgaatg gtgccggtca gtttatcagg tgtaacacct tgctcttctg cggcgacgat
    14701 ataaaacgcc agtactggta gcactgcgcc attcatggtc atcgaaaccg acattttatc
    14761 cagcgggatc tggtcgaaca ggactttcat atcttccacg gtgtcgatag cgacgcccgc
    14821 tttgccgacg tcacccgcca cgcgtgggtt atcggagtcg tagccacggt gggtggcaag
    14881 gtcaaacgca acggaaagac ctttttgccc ggcggccagg ttacggcgat aaaaagcgtt
    14941 ggactctttt gctgttgaaa aaccagcata ctgacggatg gtccacggtt gggcggtata
    15001 catagtggca cgcgggccac gaacgtaggg cggcaaacca ggaagggtac ctgtcacctc
    15061 cagattatcg agatcggctt cggtatacag cggcttgatg gcgatccctt ccgcggtttg
    15121 ctgaaccagc gagtcgacag ttttctcccg acggctcaat tccttgttgg caagctgttg
    15181 ccactcctgc tcgttagaca ttatttgctc cgtaaaacgc aatcaaaatg agggtatttg
    15241 cgcttacggt gagcttttta cgtgggtttg tcgccagcaa aaaaaatgca ttcagacgtc
    15301 gttttttgcc gcctgagatt tgtctatttt gtgatgtgat tcaaggaata caaaaagaag
    15361 tgaggacaac gcctgatatg tgctatcagg cattatttga tggattaatc ctgacgaagg
    15421 actttgtgac cataatcgag taacgcatca gtgactttac gcatcatgcg gctttcagga
    15481 gcaaagcggt gccagtagag catccgtcgt tgaaatagcc caggcgttaa gtcaatcagt
    15541 tcaccgctgg ccagctcttt ctcgatttgc aggtgcggga tcatacagca ggtggtgccc
    15601 tggcgagcaa gttgtacgaa cgcttctgaa gaattaacga tatggcaggg tacgctgcca
    15661 ggaggaagat cgaagttttg ctgcaaaaag gcctggtgca tatcgtcaag atggtcaaac
    15721 gcgaccactg gcgctttcaa taatgccgaa cgcgttacgc cgttaggaaa atatttttcg
    15781 gcaaacggtt ttgagctgac gaacagatag tcgagcgcac cgagtttatc gacaagacaa
    15841 ctcggcagcg cctgatgttg aatactcacc gcgccgacca cttcgccgcg gcgcagacgt
    15901 tcctgagtac gggtttcatc ttctacctgc aagttgaggc ggataggcga atcagccaac
    15961 acaggagcca gtgccggaag caaccacgtc gccagactgt cggcgttgac cgccagtgaa
    16021 agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc actcttcttc cagcaactcc
    16081 acctggcgca gcagtgccag cagtttttgc ccttgttccg tcgggcgcgg cggtacggta
    16141 cgcaccaaca gcggctgccc gaacatattt tccagttgct taatgcgctg tgagacggct
    16201 gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg tatcaccgca
    16261 tccagtgcct gtaatgttct gtagtccggg cgtttcattg ctgttgtctg ctcctggtaa
    16321 atgctttttc ctgcactatg acacaatttt gtgtcaggtt gcatataccg aagccgtcgg
    16381 cgggtaacat ttgtatcaat ggttaaacgc gcagcttaaa agatcaggga tttgcgctat
    16441 tgcatttcgc ttagcgactt cgcgccctgg ttatgttgaa tggcgtggcg ttattgcctc
    16501 tgttcgcacc gtggagcggc atacccagac cgttgtatgc tttatttgcg gggcgaaagg
    16561 ggatgcccgc cattgcgcga tattttatgg atgagttaac cacccggctt gccaacgggg
    16621 tctgaatcgc tttttttgta tataatgcgt gtgaaatttc ataccacagg cgaaacgatc
    16681 atgacgcagg atgaattgaa aaaagcagta ggatgggcgg cacttcagta tgttcagccc
    16741 ggtaccattg ttggtgtagg tacaggttcc accgccgcac actttattga cgcgctcggc
    16801 acgatgaaag gccagattga aggggccgtt tccagttcag atgcttccac tgaaaaactg
    16861 aaaagcctcg gcattcacgt ttttgatctc aacgaagtcg acagccttgg catctacgtt
    16921 gatggcgcag atgaaatcaa cggccacatg caaatgatca aaggcggcgg cgcggcgctg
    16981 acccgtgaaa aaatcattgc ttcggttgcg gaaaaattta tctgtattgc cgacgcctcc
    17041 aagcaggttg atattctggg taaattcccg ctgccagtag aagttatccc gatggcacgt
    17101 agtgcagtgg cgcgccagct ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg
    17161 gtgaccgata atggcaacgt gatcctcgac gtccacggca tggaaatcct tgacccgata
    17221 gcgatggaaa acgccataaa tgcgattcct ggcgtggtga ctgttggctt gtttgctaac
    17281 cgtggcgcgg acgttgcgct gattggcaca cctgacggtg tcaaaaccat tgtgaaatga
    17341 tctgacgggg gaacctcccc cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt
    17401 cacgctttta ccaggcaatt gtcgattgct ctaaataaat cctctaaacc agcatattca
    17461 tccaagaatt acctttgcgt gatatttcct caacatcgcg acgcaaacgt tcatattgcc
    17521 gcaatattat tttttgatat gttgaaaggc ggatgcaaat ccgcacacaa catttcaaaa
    17581 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
    17641 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
    17701 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
    17761 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaaa
    17821 ctggtcgcta ttggctgttt ctgtatcgga acaaaccagg ttgatctgga tgcggcggca
    17881 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
    17941 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
    18001 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
    18061 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
    18121 tatgtttact tttatgatat tgaaaacaaa ctgccgctgg gcaacgccac tcaggtacag
    18181 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
    18241 tccaccaaaa atatgatggg cgcgaaagag atttcgctaa tgaagcccgg ctcgctgctg
    18301 attaatgcct cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
    18361 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
    18421 ccatttacct ctccactgtg tgaattcgac aacgtccttc tgacgccaca cattggcggt
    18481 tcgactcagg aagcgcagga gaatatcggc ttggaagttg cgggtaaatt gatcaagtat
    18541 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
    18601 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
    18661 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
    18721 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
    18781 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
    18841 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    18901 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    18961 cgttgtgttt atttaatggg gatttacgat ggcgggacag catggggctg taacggtaaa
    19021 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    19081 attgtactgt tactgataag tttcggagct tattaacagt cagtctcaag ggaggagtca
    19141 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    19201 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    19261 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgagcac
    19321 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    19381 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    19441 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    19501 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    19561 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    19621 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    19681 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    19741 ttgtaaggtc cgatcataaa aaccaccgcc cattcccaga cgctgaccgt actcatcaaa
    19801 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    19861 cagttttggc tcatggatct tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    19921 caggaacagc aaattaccgg cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    19981 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    20041 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    20101 ttgacccatt tccagctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    20161 gcggatttct tgtcgggata atgtcagtgg gagctctggt agttgcgtca tggctggtgc
    20221 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    20281 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg aaccgagcat gctcaccaac
    20341 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    20401 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    20461 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    20521 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtctttgct
    20581 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    20641 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    20701 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    20761 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    20821 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    20881 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    20941 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    21001 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    21061 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    21121 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    21181 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    21241 cagcgttttc gatcgggctg atgcactggc aggttgggtc aatcacttcc tgcttggtct
    21301 tggcgttacg caaccgaagc tggacaaagt gaccggcgaa accggtgaag ccatcgacga
    21361 tctgcgtaac atcgcgcagc tgggctacga cgaagacgaa gatcaggaag agcttgaaat
    21421 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    21481 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    21541 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    21601 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    21661 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    21721 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    21781 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    21841 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    21901 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    21961 atgcatatgc tgatgaaatc gtgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    22021 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    22081 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    22141 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    22201 aaattcacca cgaatttaac cgccacggtg cgcgctatcc ttcctataac accattgtag
    22261 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    22321 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    22381 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    22441 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    22501 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatc ctgaaaggta
    22561 atgttgatga actgatcgct cagaacgccc atcgtccttt ctttatgcat ggccttagcc
    22621 actggttagg actggatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    22681 tggaaccggg gatggtactg accgtagagc ctggtctgta tatcgcgccg gatgcggatg
    22741 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    22801 ccggcaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    22861 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg tgggcgcgac
    22921 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    22981 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    23041 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggctg attgcgcaac
    23101 cgccatcact accgtgcatg tcagcgatcg tggtcatgct ggattcgtca ccctcgccgc
    23161 agaagattac caactggcgg cgctggggca ggttgtcgaa ttgcataatg tcgggcaacg
    23221 gctgtttgca ttgctgcgaa aagcacctgg cgtaatgctg cattgccccg atcgcgtagc
    23281 taacgttgcc cgtactcaga gtcatgttga agtgacgctg gagagcggcg agatgctgac
    23341 gggccgcgtg ctgatagccg ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    23401 tgattggcag caggagcctt acgaacaact ggcagtgatt gccaacgttg ctacttctgt
    23461 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    23521 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    23581 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tctgcctttg gctggcgact
    23641 ggggcaaatt acccacgccg gtaaacgcag tgcttatccg ctggcattaa cccgcgccgc
    23701 cagaccgatt acccatcgca ctgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    23761 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    23821 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    23881 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    23941 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    24001 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    24061 gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg
    24121 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    24181 agcgcgtacc ggaacctctg gcggtagatg cactaccaca actgcgcgtt tcggctatca
    24241 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctcgctcgta
    24301 gggccagttg ttatcacggc atggaagtgt gggacaaaga cagttttggt cacatctcgt
    24361 ttgacgatca aagcatgggc tatagccatc ttgggcacat cgttgaaaac tcagtgattc
    24421 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat tactctgttg gcccccacag
    24481 aattacagca ggtcgcctgg ggagaaaatg aaaccttcct gacgctgaaa gatggcagca
    24541 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    24601 cagatattcc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    24661 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    24721 ttttaccgct tagcgatccg catctttgct cgattgtctg gtcactgtcg ccagaggaag
    24781 cgcagcggat gcagcaggca agtgaagacg aatttaatcg cgcgttaaat atcgcttttg
    24841 ataatcgcct tggcttatgc aaggttgaga gcgcgcgtca ggtgttcccg ctgacggggc
    24901 gttatgcgcg ccagtttgcc gcacaccgtc tggcgctggt gggcgacgcc gcgcatacca
    24961 ttcacccact ggcggggcag ggcgttaatc tcggatttat ggacgccgca gagctgattg
    25021 ctgaactgaa acggttgcat cgtcagggta aagacatcgg gcagtacatt tacctgcgcc
    25081 gctatgaacg tagccgcaag cacagcgcgg cgctgatgct ggctggtatg cagggttttc
    25141 gcgatctctt ttccggcgct aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    25201 tggccgacac gcttcctggc gttaagccgc aacttatccg ccaggcaatg ggattaaacg
    25261 atttgcctga atggctgcgt taaaaatttc tcctctgttg tttatttgat acccatcaca
    25321 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    25381 ctccgttttc gcattatatt ttctaatacc atgatttttt gatttagtgt tttttgacat
    25441 ttttttagct cttaatattg tcttattcaa attgactttc tcatcacatc atctttgtat
    25501 agaaattggt gtattttttg gttttttatt ctgtcgcgat ttttgcattt tttaaccata
    25561 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    25621 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt tttgtggtga
    25681 taatttcacc atgaaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac
    25741 agactccttt gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacggct
    25801 ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg
    25861 ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct ccgcggcagc cgcacccggg
    25921 agtttctgcg ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc
    25981 tttactcggg gatgttgaat gcctctggcg gtgtgataga tgatctcatc gtctactact
    26041 ttactgaaga tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct
    26101 ggattaccca acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca
    26161 tgattgccgt gcaagggccg aatgctcagg caaaagctgc cacactgttt aatgatgctc
    26221 agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt gcaggtgggc gatctgttta
    26281 ttgccaccac tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag
    26341 cggccgattt ctggcgtgcg ctggtggaag cgggcgttaa gccctgcggt ctgggggcgc
    26401 gtgacacgct gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta
    26461 tttctccttt agccgccaac atgggctgga ctatcgcctg ggaaccgaca gatcgtgact
    26521 ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc
    26581 tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc
    26641 agggcaacca gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca
    26701 gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca
    26761 accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg
    26821 cgtgatttac ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag
    26881 caaagaacac gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtattaccga
    26941 acatgctcag gagctgttag gcgatatggt gtttgttgac ctgccggaag tgggcgcaac
    27001 ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta aaagcggcgt cagacattta
    27061 tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact
    27121 ggtgaacagc gaaccgtatg caggcggctg gatctttaaa atcaaagcca gcgatgaaag
    27181 cgaactggaa tcactgctgg atgcgaccgc atacgaagca ttgttagaag atgagtaacg
    27241 gctttattcc tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac
    27301 tctcttcgta aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga
    27361 cacagacgtt aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg
    27421 acgccgcgca acagcaagaa atgctgaatg ccgttggtgc acaatcgtta aacgcgctga
    27481 ccggccagat tgtgccgaaa gatattcagc ttgcgacacc accgcaggtt ggcgcaccgg
    27541 cgaccgaata cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca
    27601 cgtcttacat cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca
    27661 tgctggaaaa tccgggctgg tataccgcgt acactccgta tcagcctgaa gtctcccagg
    27721 gccgccttga agcactgctc aacttccagc aggtaacgct ggatttgact ggactggata
    27781 tggcctctgc ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg gcgatggcga
    27841 aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt ggcttccaat gtgcatccgc
    27901 aaacgctgga tgtggtccgt actcgtgctg aaacctttgg ttttgaagtg attgtcgatg
    27961 acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt gctgttacag caggtaggca
    28021 ctaccggtga aattcacgac tacactgcgc ttattagcga actgaaatca cgcaaaattg
    28081 tggtcagcgt tgccgccgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg
    28141 gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc tacggtggcc
    28201 cacacgcggc attctttgcg gcgaaagatg aatacaaacg ctcaatgccg ggccgtatta
    28261 tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg
    28321 agcaacatat tcgtcgtgag aaagcgaact ccaacatttg tacttcccag gtactgctgg
    28381 caaacatcgc cagcctgtat gccgtttatc acggcccggt tggcctgaaa cgtatcgcta
    28441 accgcattca ccgtctgacc gatattctgg cggcgggcct gcaacaaaaa ggtctgaaac
    28501 tgcgccatgc gcactatttc gacaccttgt gtgtggaagt ggccgacaaa gcgggcgtac
    28561 tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gccgtgggga
    28621 tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagt gtgctgctgg
    28681 gcgataacca cgggctggag atcgacacgc tggacaaaga cgtggctcac gacagccgct
    28741 ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg tttaatcgct
    28801 atcacagcga aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc
    28861 tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga
    28921 tgatcccaat cacttggacg gaatttgccg aactgcaccc gttttgcccg ccggaacagg
    28981 ctgaaggtta tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt
    29041 acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg cgaatacgcg ggcctgctgg
    29101 cgattcgtca ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatcccgg
    29161 cttctgcgca cggaactaac cccgcttctg cacatatggc aggaatgcag gtggtggttg
    29221 tggcgtgtga taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa gcggaacagg
    29281 cgggcgataa cctctcctgt atcatggtga cttatccttc tacccacggc gtgtatgaag
    29341 aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg
    29401 gcgcgaacat gaacgcccag gttggcatca cctcgccggg ctttattggc gcggacgttt
    29461 cgcacctcaa cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg
    29521 gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc gggtcatagc gtggtgcaaa
    29581 tcgaaggcat gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta
    29641 tcctgccaat cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa
    29701 gccaggtggc aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg
    29761 tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc
    29821 tgaaagaaga aaccggcatc agcgagctgg acattgccaa gcgcctgatc gactacggtt
    29881 tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat
    29941 ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa
    30001 ttgaccaggt gaaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccgc
    30061 acattcagag cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcggtat
    30121 tcccggcagg cgtggcagac aaatactggc cgacggtgaa acgtctggat gatgtttacg
    30181 gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt
    30241 cgagtatttt ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt acaaaagtgc
    30301 tcagacagtc ccctcgcccc tttggggaga gggttagggt gaggggaaca ggccggcact
    30361 ggcgcgaata tttaccctca ccccgaccct ctccctgaaa gggcgagggg gaaaagcgtg
    30421 ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt
    30481 tttctcggaa aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg
    30541 ggcaactgca ttactgttgg cgcaagaagg gtatacggtg gcggttaatt atcagcaaaa
    30601 cctccacgcg gcgcaggaag tgatgaactt aataatgcaa gccggtggca aagcattcgt
    30661 gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg atgtttacag caatcgatca
    30721 gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac
    30781 cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata
    30841 ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg
    30901 cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg aatatgttga
    30961 ttacgcggca tcgaaagggg cgattgattc gttaaccacc ggactatcgc tggaagtcgc
    31021 cgcgcagggg atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc
    31081 cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg
    31141 tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta
    31201 cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc
    31261 ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc
    31321 aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc
    31381 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa
    31441 tgacacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt
    31501 cactgctttt ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
    31561 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
    31621 cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
    31681 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga
    31741 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
    31801 aagataatcg caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt
    31861 aaatccgcga cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac
    31921 atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
    31981 acatgagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt
    32041 aatgcgacgc gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg
    32101 gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc
    32161 aaacagcggc gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt
    32221 gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa
    32281 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
    32341 gagggttatc accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa
    32401 cttcagccct tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc
    32461 gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
    32521 gtgaccataa aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt
    32581 ggtgatttcg cgcggcacgc cgtgtgcgcc acaggtcaga acgtcacaaa tgctcggtcc
    32641 tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa
    32701 gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta
    32761 caggaaatag taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat
    32821 tctggctggg cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg
    32881 tgatgtgctt cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac
    32941 cgcaacctca accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat
    33001 gaccctgcct gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta
    33061 tatgattgaa tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt
    33121 taatttcatg atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt
    33181 tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg
    33241 agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg
    33301 ccaacagcgt cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac
    33361 tggttcaggc ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg
    33421 gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac
    33481 gggcaaaaat gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa
    33541 cctacacgcc gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg
    33601 ttatctggag cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca
    33661 aaattttatc tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg
    33721 aaatggcagt taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt
    33781 attcgctcgg ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc
    33841 acggcttcgt gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc
    33901 aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc
    33961 ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt
    34021 tgtgataaag gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata
    34081 aatgcctgat gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc
    34141 agtgtattcg catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg
    34201 acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta
    34261 cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg
    34321 gcactacctg ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc
    34381 accatctctt gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga
    34441 ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct
    34501 tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta
    34561 tcggtcagca tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt
    34621 tcaaaccata gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt
    34681 tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca
    34741 cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc
    34801 agctgcggtt cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac
    34861 agacgtaaat tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga
    34921 tcttctgcca tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc
    34981 gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg
    35041 gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt
    35101 gcttaacggc gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca
    35161 ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg
    35221 tgaattaatt tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa
    35281 cacgttacaa tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa
    35341 caaagcccga attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat
    35401 gccgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg
    35461 tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc
    35521 cgatgcagaa ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc
    35581 tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg
    35641 ggctggttgc cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga
    35701 tggtgttact ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc
    35761 gccaggggga aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga
    35821 gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg
    35881 gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg
    35941 atttacggcg gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat
    36001 gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg
    36061 caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga
    36121 cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc
    36181 gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg
    36241 agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata
    36301 attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg
    36361 atttcaccaa aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat
    36421 ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg
    36481 attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc
    36541 ataacgtaag cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta
    36601 gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac
    36661 aggagctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg
    36721 aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc
    36781 gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc
    36841 tggagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt
    36901 tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac
    36961 cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac
    37021 aggcgccatt aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt
    37081 atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc
    37141 gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg
    37201 aagaggcggt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg
    37261 gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct
    37321 tctggcaccg cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac
    37381 cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg
    37441 tggtgcagat gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg
    37501 ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa
    37561 gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag
    37621 gctttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa
    37681 gcagcgatat tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg
    37741 tgttgtacat caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg
    37801 gcacggctcc ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa
    37861 aagagatgat cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata
    37921 ttacctgtgg ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga
    37981 tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa
    38041 tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta
    38101 aaagcgtcgc accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc
    38161 agcttggcgt tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt
    38221 accagccgcc gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta
    38281 aataattcgc gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga
    38341 cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag
    38401 tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt
    38461 cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt
    38521 cggtgatttc gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc
    38581 gcttggttgc agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt
    38641 aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga
    38701 taacggtatt tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat
    38761 tgttaccgat caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc
    38821 taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta
    38881 tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat
    38941 cgcaattcct aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt
    39001 cgtgccgctg gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc
    39061 cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa
    39121 actagccgcc agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact
    39181 ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt
    39241 gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca
    39301 ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg
    39361 gctggcaatg tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat
    39421 caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa
    39481 aggttccggt cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac
    39541 actctacccc ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct
    39601 ggaagaggat aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct
    39661 ggccccttcg ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat
    39721 gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga
    39781 gccgctgttt gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt
    39841 gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt
    39901 cgataccgct ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga
    39961 tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat
    40021 ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc
    40081 gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa
    40141 tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg
    40201 ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg
    40261 aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca
    40321 ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag
    40381 aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa
    40441 aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt
    40501 acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg
    40561 agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt
    40621 cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt
    40681 acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact
    40741 ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg
    40801 atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg
    40861 cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg
    40921 ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga
    40981 agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg
    41041 cgatggaaga taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg
    41101 atgactcccg cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc
    41161 tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca
    41221 acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga
    41281 aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt
    41341 tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag
    41401 aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta
    41461 tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg
    41521 cccgtgacga tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg
    41581 acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact
    41641 gtaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct
    41701 tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat
    41761 cccgtaacac ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga
    41821 accgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg
    41881 cgcgtccgtt tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc
    41941 cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc
    42001 gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac
    42061 tctacatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta
    42121 ctctggcaca ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact
    42181 tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa
    42241 ccgacatggc ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta
    42301 tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg
    42361 cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc
    42421 tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg
    42481 gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct
    42541 tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag
    42601 attacgttac cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg
    42661 accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg
    42721 cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt
    42781 ttttatttaa gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc
    42841 atcaccatcc acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct
    42901 aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg
    42961 cgtgcggcaa ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg
    43021 tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg
    43081 caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag
    43141 ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc
    43201 cccttggcat taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt
    43261 gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt
    43321 tccgtttgca taatttctca catgtaattc tgatcactca tcattataga taattgaaga
    43381 gtgaaatgat taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac
    43441 aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg
    43501 caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag
    43561 cgcgcattaa aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga
    43621 agccacatac gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg
    43681 tcagcatgat gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg
    43741 agcataatgc cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg
    43801 tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa
    43861 cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg
    43921 aacatcacta gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc
    43981 atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg
    44041 ctatgaacgc gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag
    44101 ctgttaaacg tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata
    44161 tcgtgtgaag agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac
    44221 tcgataaaca cgatgattaa cacggcagtc atggtcagaa tggaaacggg atcgaaaatc
    44281 ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg aggcatcatg tagcccggat
    44341 aaattgactt cattcatcat ccacgaaagt aaaaagccaa atacaatccc cagtaatacg
    44401 gcgacgttgg acataaatcc tttcgcatag cgagtaatga gcaagataaa aattaagacg
    44461 gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt acctccagcg
    44521 gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac cacaccggta
    44581 accagtggcg ggaataaagg catcaagcga ccaataagtg gcgctaataa tgtggtgata
    44641 aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg gttcatccca
    44701 atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg caggcggatc
    44761 cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca gcaaaagaga
    44821 tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc accaatcata
    44881 agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc gagtatgatc
    44941 agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg cccagaagat
    45001 gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg ccagaataaa
    45061 aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg ccagtttttg
    45121 cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt gcttaaacca
    45181 cgaaaatgac aagagaaact gacaatggaa ttatctgtaa ttttctcata ttgatatttc
    45241 attaatcatt ctggcatgtc gcactctcgc atttaatcgt ttttatctgg atagcgctct
    45301 tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat aatttgaact
    45361 cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt ttattgcatc
    45421 gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat acgtttatcg
    45481 ctgctgaagc tgcggaatgt ataggctgtc a
//
LOCUS       DAJTMX010000017        44228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTMX010000017 DAJTMX010000000
VERSION     DAJTMX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.017"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(31..918)
                     /gene="ugpA"
                     /locus_tag="OO145_000961"
     CDS             complement(31..918)
                     /gene="ugpA"
                     /locus_tag="OO145_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5466164.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(1016..2332)
                     /gene="ugpB"
                     /locus_tag="OO145_000962"
     CDS             complement(1016..2332)
                     /gene="ugpB"
                     /locus_tag="OO145_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5466165.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(2729..3442)
                     /gene="livF"
                     /locus_tag="OO145_000963"
     CDS             complement(2729..3442)
                     /gene="livF"
                     /locus_tag="OO145_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5466166.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(3444..4211)
                     /gene="livG"
                     /locus_tag="OO145_000964"
     CDS             complement(3444..4211)
                     /gene="livG"
                     /locus_tag="OO145_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5466167.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(4208..5485)
                     /gene="livM"
                     /locus_tag="OO145_000965"
     CDS             complement(4208..5485)
                     /gene="livM"
                     /locus_tag="OO145_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5466168.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(5482..6408)
                     /gene="livH"
                     /locus_tag="OO145_000966"
     CDS             complement(5482..6408)
                     /gene="livH"
                     /locus_tag="OO145_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5466169.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(6456..7565)
                     /gene="livK"
                     /locus_tag="OO145_000967"
     CDS             complement(6456..7565)
                     /gene="livK"
                     /locus_tag="OO145_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5466170.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            7989..8372
                     /gene="panM"
                     /locus_tag="OO145_000968"
     CDS             7989..8372
                     /gene="panM"
                     /locus_tag="OO145_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5466171.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(8571..9674)
                     /gene="livJ"
                     /locus_tag="OO145_000969"
     CDS             complement(8571..9674)
                     /gene="livJ"
                     /locus_tag="OO145_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5466172.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(9945..10799)
                     /gene="rpoH"
                     /locus_tag="OO145_000970"
     CDS             complement(9945..10799)
                     /gene="rpoH"
                     /locus_tag="OO145_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5466173.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(11044..12102)
                     /gene="ftsX"
                     /locus_tag="OO145_000971"
     CDS             complement(11044..12102)
                     /gene="ftsX"
                     /locus_tag="OO145_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5466174.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(12095..12763)
                     /gene="ftsE"
                     /locus_tag="OO145_000972"
     CDS             complement(12095..12763)
                     /gene="ftsE"
                     /locus_tag="OO145_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5466175.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(12766..14262)
                     /gene="ftsY"
                     /locus_tag="OO145_000973"
     CDS             complement(12766..14262)
                     /gene="ftsY"
                     /locus_tag="OO145_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5466176.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            14412..15008
                     /gene="rsmD"
                     /locus_tag="OO145_000974"
     CDS             14412..15008
                     /gene="rsmD"
                     /locus_tag="OO145_000974"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5466177.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            14998..15267
                     /locus_tag="OO145_000975"
     CDS             14998..15267
                     /locus_tag="OO145_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5466178.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(15270..15629)
                     /locus_tag="OO145_000976"
     CDS             complement(15270..15629)
                     /locus_tag="OO145_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5466179.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            15770..16396
                     /locus_tag="OO145_000977"
     CDS             15770..16396
                     /locus_tag="OO145_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5466180.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            16470..18668
                     /gene="zntA"
                     /locus_tag="OO145_000978"
     CDS             16470..18668
                     /gene="zntA"
                     /locus_tag="OO145_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5466181.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(18770..19015)
                     /gene="tusA"
                     /locus_tag="OO145_000979"
     CDS             complement(18770..19015)
                     /gene="tusA"
                     /locus_tag="OO145_000979"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5466182.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            19236..19901
                     /locus_tag="OO145_000980"
     CDS             19236..19901
                     /locus_tag="OO145_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5466183.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            19974..20531
                     /gene="dcrB"
                     /locus_tag="OO145_000981"
     CDS             19974..20531
                     /gene="dcrB"
                     /locus_tag="OO145_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5466184.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(20535..21752)
                     /locus_tag="OO145_000982"
     CDS             complement(20535..21752)
                     /locus_tag="OO145_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5466185.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            21886..22935
                     /locus_tag="OO145_000983"
     CDS             21886..22935
                     /locus_tag="OO145_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5466186.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            22990..23577
                     /gene="acpT"
                     /locus_tag="OO145_000984"
     CDS             22990..23577
                     /gene="acpT"
                     /locus_tag="OO145_000984"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5466187.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            23688..25262
                     /gene="nikA"
                     /locus_tag="OO145_000985"
     CDS             23688..25262
                     /gene="nikA"
                     /locus_tag="OO145_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5466188.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            25262..26206
                     /gene="nikB"
                     /locus_tag="OO145_000986"
     CDS             25262..26206
                     /gene="nikB"
                     /locus_tag="OO145_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5466189.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            26203..27036
                     /gene="nikC"
                     /locus_tag="OO145_000987"
     CDS             26203..27036
                     /gene="nikC"
                     /locus_tag="OO145_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5466190.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            27036..27800
                     /gene="nikD"
                     /locus_tag="OO145_000988"
     CDS             27036..27800
                     /gene="nikD"
                     /locus_tag="OO145_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5466191.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            27797..28603
                     /gene="nikE"
                     /locus_tag="OO145_000989"
     CDS             27797..28603
                     /gene="nikE"
                     /locus_tag="OO145_000989"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5466192.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            28609..29010
                     /gene="nikR"
                     /locus_tag="OO145_000990"
     CDS             28609..29010
                     /gene="nikR"
                     /locus_tag="OO145_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5466193.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(29130..29489)
                     /locus_tag="OO145_000991"
     CDS             complement(29130..29489)
                     /locus_tag="OO145_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5466194.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(29486..29761)
                     /locus_tag="OO145_000992"
     CDS             complement(29486..29761)
                     /locus_tag="OO145_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5466195.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(29820..30944)
                     /locus_tag="OO145_000993"
     CDS             complement(29820..30944)
                     /locus_tag="OO145_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5466196.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(30944..33679)
                     /gene="rbbA"
                     /locus_tag="OO145_000994"
     CDS             complement(30944..33679)
                     /gene="rbbA"
                     /locus_tag="OO145_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5466197.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(33676..34743)
                     /locus_tag="OO145_000995"
     CDS             complement(33676..34743)
                     /locus_tag="OO145_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5466198.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(35109..36731)
                     /locus_tag="OO145_000996"
     CDS             complement(35109..36731)
                     /locus_tag="OO145_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5466199.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(36993..38582)
                     /locus_tag="OO145_000997"
     CDS             complement(36993..38582)
                     /locus_tag="OO145_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5466200.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            38967..39998
                     /locus_tag="OO145_000998"
                     /pseudo
     CDS             38967..39998
                     /locus_tag="OO145_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(40085..40561)
                     /locus_tag="OO145_000999"
     CDS             complement(40085..40561)
                     /locus_tag="OO145_000999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466201.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(40554..41318)
                     /locus_tag="OO145_001000"
     CDS             complement(40554..41318)
                     /locus_tag="OO145_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5466202.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NGMHYLLGEGQRYLSRYIKSSNDETYAICSEVLLYD"
     gene            41564..42325
                     /locus_tag="OO145_001001"
     CDS             41564..42325
                     /locus_tag="OO145_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5466203.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(42432..42932)
                     /locus_tag="OO145_001002"
     CDS             complement(42432..42932)
                     /locus_tag="OO145_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466204.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(43083..>44228)
                     /locus_tag="OO145_001003"
     CDS             complement(43083..>44228)
                     /locus_tag="OO145_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5466205.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        10773 a        11667 c        10736 g        11052 t
ORIGIN      
        1 tgaatatcgt cagccacgga cggttctcaa tcattggtaa cgcaccttgc tttcaacata
       61 gcggaactgc accaccgtca gcacgatgac gaggaacatc agcaccatcg actgtgcggc
      121 agacgaagcc agatccagtc cggtaaaacc ttcgcggtag atcttataaa tcagcgtcgt
      181 ggtggcctga accggcccgc cggacgtggc ggcgtcgatc accgggaagg tgtcgaagaa
      241 ggcatacacc aggttcacta ccagcaggaa gaaactcacc ggggcgataa gcggcagcgc
      301 aatcttaaag aagcggcgaa tcggccctgc accgtcgatg gctgcggctt cgatcaacga
      361 acggggaatg gattgcagcg cggcatagaa gaacagaaag ttgtagctga tttgcttcca
      421 caccgaggca aacaccacca gaaacatcgc ctgaccgcta ttttgcgcgt ggttccagtc
      481 gtagccgaac tcagcgagaa aatgggtgat cagcccgcga ccggggttaa acaggaagat
      541 ccacaatacg gcggcaacgg cgggagccac ggcatacggc agcaacatta aggtttggta
      601 gaaacgactg ccgcgcacga tgtactccac cagcgccgca aagaacagcg ataccagcaa
      661 accgctgacg gtgacaaagg tgctgaattt tatcgtcgtc cagaaggagt cgagataata
      721 gctgtcatga aacagcgtga cgaagttatc cagaccgaca aactggctgg agaaaccaaa
      781 cggatcgacg ctttgtagcg agtaccacaa cgcttcgccc gcaggccaga taaaaaagat
      841 aacggtgatg atgagctgcg gcgcgaccag cagataaggc agccagcgcg agcggaacac
      901 cggacgggat gatgacatta cggttaattc ctgaactgtg ccggatgcgc ttcgcttatc
      961 cggcctacac gcgatgccag gtcagataag cgaaacgcat ccggcattac actgattaag
     1021 acttcgtcga tttctcaaag cggcgcagta actggttccc acgctcaact gcggtatcca
     1081 gcgcctgctg tggcgtcttc ttaccggtcc acacgctctc aagctcttca tccacaatca
     1141 cgcggatctg cggcatgttg cccagacgaa gccctttggt gaacggcaac ggcggcttgt
     1201 tcagcatctg acgcgtcgcg gtatccgcac ctgggttttt ctcgtaaaag ccctgctcac
     1261 gggtcaggtc atacgccgct ttggtaattg gcagataacc ggttttctga tgccactcag
     1321 cagcgttttc tggcttcgcg aggaagtcga gaaacttcgc cacgccggta tacgtttctt
     1381 tatctttgcc ctgcatcacc cacaggctgg ctccgccgat aatggcgttt tgtggcgcat
     1441 ctttcgcatc ggcgtcgtaa ggcatcatac ctacgccgta gttaaatttg gcgtactcgc
     1501 gaatgttggc aagagaaccg gaagaggcgg tggtcatcgc gcaatcaccg ttatagaact
     1561 tctcggtgga ttcatcctta cgaccgacgt agctgaagtc gcccttcttg ttcatctcct
     1621 cgagcatggc gatgtgtttc acctgctccg gcttgttgaa ctccagcacc gcgtccgtgc
     1681 cgtcaaagcc gttgtttttg ctggcaaacg gcagaccgtt ccaggcgcta aagttttcca
     1741 gttggatcca gccctgccag ccgctggcgt agccgcactt aataccggag gctttcagtt
     1801 ttgcggaata gtccgccaga tcctgccagg ttttcggcgg ctgttccggg tctaaccccg
     1861 ctttcttgaa ggcgtctttg ttgtaataga gaacggaagt cgagctgttg aacggctggg
     1921 agagtaagtg gccggttttg ctgtcggagt agtaaccgga aaccgtcggc acaaactgcg
     1981 actcatcgaa ctgaatccct gcctctttaa acacgtcata caccggttta atggctttcg
     2041 acgccatcat ggtggcggtg ccaacttcat aaacctgcaa aatagccgga gcgttgccgg
     2101 tacgaaatac ggcaatcccc gcgcttaaat tctgttcgta gttgccttta taggtcggta
     2161 caattttgta atccggattt tcggcgttaa aacgttgggc cagagaatcc acctctttac
     2221 ccagttcccc ttccatagaa tgccagaacg gaatggtcgt cactgcctgt gcattcccca
     2281 ttaacgccag tccgagcgcc agtgctgaag ctgtataatg taacggtttc atcgtttatc
     2341 tctcttgttg taccgaatgc gcgaattcac gcgttttatg ctcgcggggt aacatgacat
     2401 gctcgaatta cagaaaaata acttttttgt tacatttgta agatagtaag gtgtcagaaa
     2461 gatgacaagg cggtgacggc gtgggtgagg gaaaatggga gatggggcac ggataagcgg
     2521 aaaaatagaa ggtctgaatc aaactctaca gattactcat cgtttcatgc cggatgcggc
     2581 gtaaacgcct tatccggcct acaagatcgt gcaaattcaa tatattgcaa ctcacccagt
     2641 aggcctgata agcgcagcgc atcaggcaat tttgcatttg tcacctgtct caaaggagtc
     2701 ttttgactcc ttatcaatca acgtgttatt acccgcctaa atacgcactt ctcactgctt
     2761 cgttcgccag cagcgcgtcg ccggtatcgg agagcaccac atggccgttt tccagcacgt
     2821 agccgcgatc cgccagcttt agcgcctggt tggcgttctg ctcgacgagg aagatagtca
     2881 tcccctgctc gcgcagctgc tcgatggtgt cgaaaatttg ctggatgata atcggcgcaa
     2941 gaccgagcga tggctcatca agtaacaaca aacgcggatt gctcatcagc gcgcgaccaa
     3001 tcgccagcat ctgctgttca ccgccggaca tggtgcccgc ccgctgaaca cggcgctcat
     3061 gcagacgtgg aaacagttca tacacccact ttatgcgctc ctgaaactgg tcacgttcgg
     3121 caaagaagcc gcccatcgcc aggttctctt ccaccgtcat ccgcgagaag acgcgacgcc
     3181 cttccgggac aatcgctacc gcttcgcgca tgattttcgc cgtctgccag tcggtaatgt
     3241 ctttatcatc aaacacaatt cgcccgctgg tggcgcgcgg atcgccgcat aacgtgccga
     3301 gcaaggtggt tttccccgcc ccgttcgcgc caatgagagt gacaatctcg ccctgattga
     3361 tatgcaggct cacctcatgc agcgcctgga ttttgccgta gtgggcgctg actttgtcaa
     3421 aggacaacat gactttttcc atcttatgcc tcacctaaat aggcacggat cacgtccggg
     3481 ttattacgga tttgctccgg cgtaccgttt gccagtggcg tcccctgatt gaccacataa
     3541 attcggtctg aaatccccat caccagcttc atatcgtgtt caatcaacaa aatagtggtg
     3601 ttgtgatgat tgcgcagttc ggcaatcagc tcatccagct ctttggtttc ttttgggtta
     3661 agacctgccg ccggttcgtc gagcattaaa atctccggct gcgtcaccat gcagcgggca
     3721 atctccagac ggcgttggtc accataggcc aggttactcg cctgacggtt ggcgtgttcc
     3781 agcaaaccaa tgcgctcaag ccaggtggcg gcgcggtcga gcgcttcgct ctgggcgcga
     3841 cggaaagaag gcgttttcaa caggccagag aacagcccgg ttttcagttg ctgatgctgc
     3901 gccaccagca ggttttcaat taccgtcatt tcacggaaca gacgcacatg ctggaaggtg
     3961 cgcaccacgc ccatgcgagc aatttgctgc cccggtaaac cttcaagatg ctgatcgcgc
     4021 agtaaaatgg tgccgccggt gggtttgtag aaaccggtca ggcagttaaa aaccgtggtt
     4081 tttccggcac cgttagggcc gattaacgag acgatttcct gcgggtacag ttcaagattg
     4141 acgttgttca ccgccagcag gccgccgaag cgcatcatca gaccgttaac agataataat
     4201 ggctgactca tgcctgctct cctttcgctg cgccgttttt cagcttcagt tgcgggcgcg
     4261 tcatgggcag caaaccctgc ggacgccaga tcatcatcag caccatcaaa ccaccgagca
     4321 ttaacatgct gtattcgttg aaatcacgca tcaattcgcg cgacaccacc agcagaacag
     4381 ccgccagaat cactgcaaat tgcgagccca taccgccgag caccactatc gccagcacaa
     4441 aggccgattc agcaaaggtg aaggattccg ggctgacaaa gccctgacgc gccgcaaaca
     4501 gcgttccggc aaaaccggca aatgcggcgc tgatggtaaa agcagtcagc ttgatacggc
     4561 gcgggcttaa gcccagcgaa cgacaggcga tctcgtcttc acgcaacgct tcccacgcgc
     4621 ggcccagcgg catccgcagc aggcggttaa tgacaaacag gcttaacacc accagcagca
     4681 acgccaccag gtagaggaag ataacgcgat cggacggatc gtatttcagg ccaaagaaat
     4741 tactgaacgt gtcccagccg ccttcacgag cggtacggct gaactcgagt ccgaagagtg
     4801 tcggtttcgg gatctgactg attccgttcg ggccgccggt gatttcagtg ttattgagca
     4861 gcaatatgcg cacaatttcg ccgaaaccaa gggtaacgat cgccagatag tcaccgcgca
     4921 gacgcagcac cggaaaaccg agcaggaaac ccgccgccgc tgccatcagt ccggcaatcg
     4981 gcaaacaggt ccagaacccc agaccgtaat agtgattgag cagcgcaaaa gtgtaagcgc
     5041 cgatggcgta aaaaccgccg taccccagca ccagcagacc agaaagacca acaaccacat
     5101 tcagcccgag accgaggata atgtagatca tggtcagagt ggcgatatcc accgtcccac
     5161 gtgacaccat aaacggccac gccaccacaa gcaccagcag cgccacgagg aacagctttt
     5221 gcttcaccgt ggacccatca atcgcgggca gaataaactt cggcccggaa acgcttttca
     5281 accctttctg gaaagccggt cgcaaaagct ggaagaaaaa gaccaccgcc gtgccgataa
     5341 acatccactg ccaacggatg tctgaagccg tgtcgaccac cagtttggtg ccatccagct
     5401 ccagttgtac gcccataaag acgcccgcca ggacaaagaa catcgcggca gagagcagtg
     5461 ccattgcaat atgcatcggt ttcatacttt ctctacctcc gggcgaccca gaataccggt
     5521 cggcatcacc agcagcacca gaatcagcag ggcgaatgag accacatctt tatattccgt
     5581 actcagatag gcagaagaga gcgcctccgc aatccccagg atcagcccgc caatcatcgc
     5641 ccctggaatg ctgccaatcc caccgagcac cgcagcggta aaggctttca tcccggccat
     5701 aaagccgata taggggttaa tgacgcagta gaactgaccg agcagtacgc cagccactgc
     5761 cgccatcgcc gcgccaataa caaaggtcaa agcaatcacc cggtcggtgt tgatgccaag
     5821 caggctcgcc attttcagat cttccgcgca ggcacgacag gcgcgcccca tacgggaata
     5881 gcgaatgaaa atcgtcagcg ccagcatggc gaggaaggtg acaatccaga tcaccgcctg
     5941 catggtggta atagaggcag agaagttttc gctatggccc accacccact gaccgttaaa
     6001 caggctcggc agcgccacgt cgcgcgaacc ttcggtcagg ctgacgtagt tttgcaggaa
     6061 gatggacata ccgattgcgg aaataagcgc aatcaggcgc ttagagttac gcaccgggcg
     6121 gtaagctacc cgttcgatac tccagccgta ggcgctggcg atgacgattg cgccgacgaa
     6181 tcccgccgcc accagcagcc agccggtatc aatgcccatc atcatcagcg cggcgatgat
     6241 cataaatgag acgtagctgc caatcatata aacctcgccg tgggcgaagt tgatcatgcc
     6301 gataatgccg taaaccatgg tgtagccgat ggctatcagc gcgtaggtac tgcccagcgt
     6361 gacgccgtta aacatctgct gcaagaaata caaaaactgc tcagacataa ggtaaccttt
     6421 ctaaacccgc ccgcatttta cgggcggtgg gatgatcact tggctgccgt ggatgaaccg
     6481 tcggcgtgcc actggaagac accaaaatca aatcccttaa gatcgccttt ttcatcccag
     6541 ttcagcggcc caatcacggt gtttgcaccg ttagctttta aatctttcac cagcgccagc
     6601 ggctcatcgc tgccggtacg ctcaagggca gttgccagag attgcactgc cgcgtaagtg
     6661 atccagacat aaggcccgga cggatctttc ttgtctgctt tcagcgcatc aacgatgccc
     6721 tggtttgccg gatcctggtc atagcgtttt ggcatagtga ccaacatgcc ttcggcggca
     6781 tcaccggcaa tgttcgacag cgatgcgtta ccgacacctt ccggccccat aaactgggtt
     6841 ttcaggccaa cggaacgggc ttggcgcagc atctgcccca tttccgggta gtaaccgccg
     6901 tagtaaacga agtcgatatt ttcttttttc aggcgggcga tcagcgcgga gaaatctttc
     6961 tccccggcgg taataccgtc gaagaagacg acgttggcgt tagccgcttt cagcccgtcc
     7021 tgcaccgaac gcgccagccc ttcgccatac tgttgtttgt cgtgaatgat ggcgatgcgc
     7081 tggggcttca ccgtctcaag aatgtatttt gccgccgtag gcccctggga agagtccagc
     7141 ccggcagtac gcataatgtg ttgataaccg cgttgggtca gctccgggtt ggtcgctccc
     7201 ggcgagatca tcagaatacc ttcgtcttcg tagatatccg acgctggctg ggtagaggaa
     7261 gaacacagat gaccaataac gtatttaatg ccgtcgttaa cgattttgtt ggcgaccgca
     7321 acggcttgtt tcgggtcgca ggcgtcgtca tattccacgc caaccagctt gtcgccctta
     7381 attccccctt tgacattaat gtctttaatc gcctgacgcg cgccgttaaa ttccatatcg
     7441 ccccactggg caatcgggcc ggacatcgcg ccgacaacgg cgactttaat atcgtcagcc
     7501 atagcggtgt gtgaaattgc cagtgcaatc atccctgcga tgatagtttt cgcattccgt
     7561 ttcatagtca aaaatcccca ttcgtgatgt tgtgttgctt tgtttttatg tgttaacaaa
     7621 tcagactgtt ctttttttat actgcactgt ttttgcctgt ctgattaagg ggttagcgca
     7681 gtattttgtg atgatagcga ttaaaatccc tatttttcag tcgattaaga acagataata
     7741 ttctgaattt attgatagat aaacagaaaa aagtgccttt gtcagcataa aataacggca
     7801 caaagggcgg aataattcac tatcattcag gggattatgc tggacatttt tcattctcta
     7861 atgttttaac tttgtaatta ttgctgttaa aaaattaatc acctgccaaa agaaataaaa
     7921 aagagaaagc ctccgattaa attatttcgc tacactggtt ccacttttgt gatttacacg
     7981 ggttacccat gaagctgacc atcattcgat tagaaaactt tagcgaccag gaccggattg
     8041 acctgcaaaa gatctggccg gagtattccc cttcctcttt acaggttgac gataaccacc
     8101 gtatctacgc cgcgcgtttt aacgagcgtc tgcttgcggc cgtgcgggta accttaagcg
     8161 gcaccgaggg agcactggat tctctacgcg tgcgggaagt cacccgccgt cgcggtgtgg
     8221 ggcaatatct gctggaagag gttttgcgta acaatcctgg cgtttcatgc tggtggatgg
     8281 cggatgcagg cgtggaagat cgcggtgtga tgacggcgtt tatgcaggca ctgggattta
     8341 cggcacagca gggcggctgg gagaagcgtt aatcgtcaag ttgatttcaa aagtgatgac
     8401 caacgagaaa ttgcctgatg cgctacgctt atcaggccta taatgtgtgc tgcaatttac
     8461 tgatttcttt ggttcttgta ggccggataa ggcgtttacg ccgcatccgg catgaagcaa
     8521 cgtattcgat attagcaatt tggcggcaac ccaaagttgc cgattaacaa ttacttcgca
     8581 tcggtcgccg tgccgttggc gtgccagtca aatacgccga actcaaagcc ctttagatcg
     8641 cctttctcat cccaggtcag tggccccatc acggtatcca cggagttcgc ttttaggtat
     8701 ttggcgattt cagccggatc gtcagactga ttcaggcccg cctgcaaaga ttgcagcgcg
     8761 gcgtaggtgg tccagacgaa tgcgccgctt gggtcctgtt ttttcgcttt gatcgcatca
     8821 acaatgggtt tgttcgccgg aacctgatcg tagttcttcg gcttggtcac cagcagccct
     8881 tccgctgatt cgcccgcaat gttagacagc gaaacgttag ccacaccttc cggccccata
     8941 aactgagttt tcagccctgc cgcgcgtgcc tgacgcagga tttgccccat ttccgggtga
     9001 taaccgccgt agtaaacgaa gtcgatattc tcttttttca gacgcgccac cagcgttgag
     9061 aaatcttttt ccccggcggt gataccatca aagaacacca cgttagcatt gcctttcttc
     9121 aggccgtcct gcaccgctcg tgccagacct tcgccgtatt gctgtttgtc gtgaacgata
     9181 gcaatacgct gcggtttcac tttctcaaga atatatttcg ccgccgtcgg cccctggtcg
     9241 gagtccaggc cggtggtgcg caggatcagc tgatagccac gggcggtcag ctccggcgcg
     9301 gttgccgctg gggtgatcat taaaatgcct tcgtcttcgt agatgtcaga cgcaggctgc
     9361 gttgatgaag aacagaggtg accaatcaca tatttaatgc cgtcgttaac gactttgttc
     9421 gccaccgcaa ccgcctgttt cgggtcacag gcatcgtcat attttacgat ttgcagtttg
     9481 ttgcctttaa tgccgccttt agcgttgata tccgcaaccg cctgctctgc gccggtaaac
     9541 tcctggtcac cgtactgcgc aaccggaccg gacattgcac ccacgaccgc gactttaata
     9601 tcttctgcca gagccatatt gctgaatgcc agcgcgatac atcctgccag taacgcttta
     9661 ccctttatgt tcatcctgag aatccccatt cttctggtta ttacgtgtgt tgtgatgttg
     9721 ttgttcagca ctttatttcg ttttatgcat gactacccgt gctttagcag catactctgc
     9781 taaaacatac ccgattttta tgatattgga atagctattt tgacagttta ttaacaatct
     9841 gcgtggggat tggcgttttg ccggaggggg aattgattat tgtagaggcc caaaaaacaa
     9901 aaaccccgga ctctcatcca gggttctctg cttaatagcg gaaattacgc ttcaatagcg
     9961 gcgcgaagtt ttttcatcgc gttcttttcc agttggcgca cacgttcagc ggaaacaccg
    10021 taacggtcag ccagttcctg caacgtggac ttgttgtctt cgtccagcca gcgcgcacgg
    10081 atgatgtcct ggctgcgttc gtccagacca tgcatcgcgt cggtcagacg gtttgccgcc
    10141 tgctcttccc agttatcatc ttcgatgccg tcggcaaagt tagatgattt atcctgcaga
    10201 tagagcaccg gtgccatcgg ctggctgtcg gaatcgtcgt cggaagacag gtcaaaggtc
    10261 atgtcctgtg ccgccatacg tgattccatc tcacgtacgt ctttgctggt tacgcccagt
    10321 tcacgggcca ccatttcgac ttcatcctgg ttaaaccagc ccagacgctg cttggtttta
    10381 cgcaggttga agaacagttt gcgctgcgct ttggtggtcg caactttgac gatacgccag
    10441 ttacgcagaa cgtattcgtg gatctctgct ttgatccagt ggacggcgaa ggagaccagg
    10501 cgcacaccca cttccgggtt aaaacggcgc actgctttca tcaggccgat gttaccttcc
    10561 tgaatcaaat ccgcctgtgg caggccatag cccgcataat tacgagcaat atgaacaaca
    10621 aaccgcaggt gagacaggat cagcgtttta gctgcttcca gatcgccatg gtaatgcagc
    10681 ttttcagcca gcgcccgctc ctcgtcagcc gacaacatcg gccacgcgtt agctgcccgg
    10741 atgtaggaat ccaggttgcc aactggggct aaagctaaac tttgcatttt gtcagtcatt
    10801 caaatcctct caatcgatat cttctggcgc ttcagtggta gcaacaactg tgccaaagct
    10861 taagagcaac gaggttatca ttcactgttt tatcagaccg tgattttatc cacaagttca
    10921 atgcaagctt gtgaataaat tacgcacaaa atgtgacata gagatgaaat accgggaaga
    10981 gacaacgggg tctctttccc tgctacggaa cccattgcag ggaaagagta taacacgctt
    11041 ttattattca ggcgtaaagt ggcgtaaatg ttgtaccgtg gcaagccacg ctgccaccca
    11101 gccaatcatc gagcatacca gcagcaatag caggcattca tcgaacgata aaccattgat
    11161 atcaaacttc gttccgaaaa cctgtgccac ttccgccacc gctgacgaca aacgcagtac
    11221 cagaatttct gacaaaatta atgacaacaa tgcgccagaa aatcccagca gcgcaccgcc
    11281 gtacaggaac gggcgaagga tgaaaccatc cgtcgcacca atcagtttct gcacgttgat
    11341 ggagtcacgg cgagcaaaga tgctcagacg tacgctgttg ccgatgacga ggaacactgc
    11401 cgccaccatc aacacgccga tcatcgccga aacgcgccca accagcccgg tcaacgccgc
    11461 cagacgggca aaccagctgt catccatccg cacttcgtca ataccgttaa tctgcgtgat
    11521 acgatcacgc agcgtattga gtgattccgt cccctgaaaa tcgagtttcg ggatcaccac
    11581 cgccactgcc ggaagcgggt tttcttccag catatccagc gcaccaccaa aaccagacca
    11641 gttacggaac tcgcccagtg cgtcttcacg agaaagatag ttcactttct ccacgccttg
    11701 ctcggcctgc aactgtgcca ccacgcccgc agcggcgtca tcgtccagcg ttttttgcaa
    11761 gtaaacagtg atttgcggtg acggataata ctgcgtcgcc gcctgattaa cgtttttgta
    11821 caccatataa cagacgctgg gcagcgtcag ggagatggcg ataaccatca ccgttaaaaa
    11881 cgtggcgaac ggtttgcttt tcagatcctg caatgcgccg tggaaggcat agcgcacctg
    11941 ttcgttgaaa acgttggttt tgcgatttac cggttttggc gaggatttcg cgcgtttcgg
    12001 tgcgttacga ccgccgtcgc ctgagccgcc gaccgattta cggaagcgat ccagacgccc
    12061 accaaactgc cgaatatgat tgattgcatc gcgcttattc atggcccacg cctccatgca
    12121 agtgaccatc gctcagggtg agcatgcgat aggaacgccg tgaaatcaga ttaatgtcgt
    12181 gcgttgccat caatacggtt accccaacgc ggttaaactc ttcaaacaga cgtagaatcc
    12241 cttccgacaa cgcgtcgtcc aggttaccgg tcggttcgtc cgccagcagt accgcgggct
    12301 tattcaccac cgcgcgggca atgccaacac gctgttgttc accgccggaa agctgaatag
    12361 ggaagttctt cgctttgtcc agtagcccga ctttatccag tgccgccgac acccggcgac
    12421 gaatatcgtc accgctggca ccagcgataa tcagcgggat cgccacgtta tcgtagacag
    12481 tacggtccat cagtagatgg tgatcctgga aaatcatgcc aatctggcgg cgcagaaacg
    12541 gaacttcacg gtttttcaga cgcgtgatgt catggccgct aaaccagatt ttcccggcgc
    12601 tgggccgctc aatcccacag atcagcttca ggagggtact tttccctgcg ccggaatgac
    12661 cggtcagaaa cgccatctca cccggctgca tatggaacgt aacgccctgc agcgcctgtc
    12721 tcccaccgag ataagccttg ctgacatgtt caaagcgaat cattgttaat cctctcgggc
    12781 aaaaagtgcc tctataaagt cgtccgcctt aaacggacgc aaatcctcaa tacgttcgcc
    12841 gacaccaatg tagcggatag ggataccaaa ctggtcagcc accgagaaaa ttaccccgcc
    12901 tttcgccgtg ccgtccagtt tcgttagcgt gatgccggtt aagccaacag cttcgtggaa
    12961 cagtttggcc tggcttaccg cgttctgccc ggtgctggca tcaatagtca gcataacttc
    13021 atgcggcgct tcaacgtcga gtttcttcat cacgcggacg attttcttca actcttccat
    13081 cagatgcgat ttgttctgca gacgtccggc ggtatcggca atcaggacgt cgatattacg
    13141 cgctttagct gcctgaatgg cgtcgaagat aacagaggcg gaatccgcac cggtatgctg
    13201 ggcaatcacc ggaatattgt tgcgctgacc ccagacctga agctgttcaa ccgcagctgc
    13261 acggaaagta tcgcccgccg ccagcatcac cgatttaccc tgctgctcaa actgacgcgc
    13321 cagcttacca atcgtcgtgg ttttacccac accgttgacg cccaccatca ggatcacgaa
    13381 cggcgtttta tcttcaacat tcagcggctc atcgactttc gccagaatct cgcccatctc
    13441 ttctttcagc aggccataga gcgcctcggc atcacgaagc tgcttgcggg atgcgccttc
    13501 cgtcagattg gtgataattt tacgtgtggt ttccacgccc acatcggcga tcaacagctg
    13561 ttcttccagc tcttcaaaca gatcatcgtc gattttttta ccgcggaaca ggctgataaa
    13621 tccggaaccg agattttctt tggtttttaa caggctgcgt ttcaggcgcg cgaagaagcc
    13681 ttctttggtc ggtttttctt gctcctgggc aatttctgca accggctgct cttcttctgc
    13741 cggaggaact accatcaccg cctcttctgc cgcttcggca gccagcgcct gtgcttccag
    13801 ctcttcgtcg gtgatttctt ctttagccgc ttcttcttct gccgcttcga caatctctac
    13861 ggtttccgct tcagcctgcc actcttctgg cgaaacctct tcggcgttga cgtcttccgg
    13921 caacggcagc tcttcacgtt cgatagccac tggctccggc gtttcttcta cgaccggttc
    13981 cggctgtgca acaacttccg cttcaggctg cgctttttca ctttcaacaa cctgttcagt
    14041 gacttccaca acgtcggcag caaaagtttc cgcctcggct tcagtatgcg cctgcggctg
    14101 ctcttcaacg gcttgttcag aagccttcgc aggctcttgc gcctgaacga tttcttctac
    14161 aaccggttgt tcattctgaa cttctgtctc tttttccggg gtctgctctt tttgaccaaa
    14221 gcccagccag gaaaaaaagc cacgtctttt ttctttcgcc atttgcgact acactcctcg
    14281 ctgttgcttc atggcacagc gttaacgcta tgtacatagc agctaaaaaa atgatgaaat
    14341 agtctatcac ttaacttaat tcacatcacc gcctgcaagt atgtgttatc tggcggattg
    14401 agcaatttat catgaaaaaa ccgaatcatt ccggcagcgg ccaaatccgc attattggcg
    14461 ggcagtggcg aggccgtaaa ctcccggttc ctgatagccc aggtctgcgc cccaccaccg
    14521 accgcgtacg cgaaacgttg tttaactggc tggctccggt cattgttgac gcccaatgtc
    14581 tggattgctt cgccgggagc ggcgcgctgg ggctggaagc gttatcgcgc tacgctgcgg
    14641 gggcaacgtt gattgagatg gatcgcgtgg tttctcagca gttaattaag aatctggcga
    14701 cactaaaagc aggcaatgca cgcgtggtga acagcaacgc aatgtcattc ctggcgcaaa
    14761 aaggcacacc gcataatatc gtgtttgtcg atccaccgtt ccgccgtggc ttgttagaag
    14821 agacgataaa tttactggaa gataacggct ggctggctga cgaagccctg atttatgtcg
    14881 aaagcgaagt cgaaaacggt ctgcccactg ttccggcaaa ctggtcatta catcgggaaa
    14941 aagtggcggg tcaggtggct tatcggctgt atcaacacga agcacaagga gaaagtgatg
    15001 ctgattaata ttggtcgttt gttaatgctc tgcgtttggg gatttttaat cctcaatctg
    15061 gtgcatccct tcccgcgccc gctaaatatt ttcgttaacg tggcgctgat ttttaccgtg
    15121 ctgatgcatg gtatgcagct ggcgctattg aaatccactt taccgaaaga tggcccgcag
    15181 atgaccaccg ccgaaaaggt gcggattttc cttttcggcg tgtttgaact gctggtctgg
    15241 cagaagaaat ttaaagttaa aaaataacct tattgttcgc caacaaagct aacaaagcgc
    15301 gttcctttat agctcagcgt acctttatcg cccactgtca gggcgtggta ctgctgggcg
    15361 tcgaggcgaa acgtctgctc cattcctccg ctttgcggtt tgaagcttgc ctcatagcgc
    15421 atactggtgc ctgccggagt cacttcctgc tggcgcgaac ggcgatcgtt aatcggtttt
    15481 tcccgcttgt tgctcaccac caccagcttt tgctggagcg gagccatatc attatcagct
    15541 ttttcccgtc gctgttgcat aaaacgaaac gatgcggcga cgacaattaa gccaatgata
    15601 acaataaaga aaagaggtgg tttactcatc tttatccctc atcggaaaat gcggaattaa
    15661 gcataccctg ccagttatgg tgttgtcatc cgtccaccct cgccactaaa ctggaagcaa
    15721 gaccgtaggc attctgctta cgaaaaaata acgaattcaa ggaactaaga tgctttggtc
    15781 gtttatcgct gtctgtcttt ccgcatggct atctgtggat gcatcgtatc gtgggccaac
    15841 ctggcaacgc tgggtgttta aaccgttaac ccttcttctc ctgctgttac tggcctggca
    15901 agcaccgatg ttcgacgcca ttagctatct ggtgctggcg ggactgtgcg cctcactgct
    15961 gggcgatgcg ctaaccctgt tgccacgtca acgtctgatg tacgccatcg gcgcgttttt
    16021 cctctcgcac ctgctgtaca ccatctattt cgccagtcag atgacgctct ctttcttctg
    16081 gcctctgcca ctggtgttgc tggttctggg tgcgctgcta ctggcgatta tctggacgcg
    16141 gctggaagag taccgttggc ctatctgcac gtttatcggc atgacgctgg tgatggtgtg
    16201 gctggcaggt gaactgtggt tcttccgtcc gaccgctccg gcgctctctg cgtttgttgg
    16261 cgcttcgttg ctgtttatca gtaactttgt ctggctgggg agccactatc gccgacgctt
    16321 ccgtgcggat aacgcgattg ctgcggcctg ctactttgcc ggtcacttcc tcatcgtccg
    16381 ctcactgtat ctctgataaa acttgactct ggagtcgact ccagagtgta tccttcggtt
    16441 aatgagaaaa aacttaaccg gaggatgcca tgtcgacacc tgacaatcac ggcaagaaag
    16501 cccctcaatt tgctgcgttc aaaccgctaa ccacggtaca gaacgccaac gactgttgct
    16561 gcgacggcgc atgctccagc tcgccaacgc tctctgaaaa cgtctccggc acccgctata
    16621 gctggaaagt cagcggcatg gactgcgccg cctgtgcgcg caaggtagaa aatgccgtgc
    16681 gccagcttgc aggcgtgaat caggtgcagg tgttgttcgc caccgaaaaa ctggtggttg
    16741 atgccgacaa tgacatccgt gcccaggttg aatctgcggt gcaaaaagcg ggctattccc
    16801 tgcgcgatga gcaggccgcc gacgagccac aagcatcgcg cctgaaagag aatctgccgc
    16861 tgattacgct tatcgtgatg atggcaatca gctggggtct ggagcagttc aatcatccgt
    16921 tcgggcaact ggcgtttatc gcgaccacgc tagttgggct gtacccgatt gctcgtcagg
    16981 cattacggct gatcaaatcc ggcagctact tcgccattga aaccttaatg agcgtagccg
    17041 ctattggtgc actgtttatt ggcgcaacgg ctgaagctgc gatggtgttg ctgctgtttt
    17101 tgattggtga acgactggaa ggctgggccg ccagccgcgc gcgtcagggc gttagcgcgt
    17161 taatggcgct gaaaccagaa accgccacgc gcctgcgtaa cggtgagcgg gaagaggtgg
    17221 cgattaacag cctgcggcct ggcgatgtga ttgaagtcgc cgcaggtggg cgtttgcctg
    17281 ccgacggtaa actgctctca ccgtttgcca gttttgatga aagcgcccta accggagaat
    17341 ccattccggt ggagcgcgcg acgggcgata aagttcctgc aggagccacc agcgtagacc
    17401 gtctggtaac gctggaagtg ctgtcagaac cgggtgccag cgccattgac cggattctga
    17461 aactgatcga agaagccgaa gagcgccgcg cccccattga gcggtttatc gaccgtttca
    17521 gccgtattta tacgccagcg attatggccg tcgccctgct ggtaacgcta gtgccgccgc
    17581 tgctgtttgc cgccagctgg caggagtgga tttataaagg tctgacgctg ctgctgattg
    17641 gttgcccgtg tgcgttagtt atctcaacgc ctgcggcgat aacctccggg ctggcagcag
    17701 cagcgcgtcg tggggcgtta attaaaggcg gtgcggcgct ggaacaacta ggtcgtgtta
    17761 cccaagtggc gtttgataaa accggtacgc tgaccgtcgg taaaccgcgc gttaccgcga
    17821 ttcatccggc aacgggtatt agtgaatctg aactgctgac actggcggcg gcggtcgagc
    17881 aaggcgcgac gcatccactg gcgcaagcca tcgtacgcga agcacaggtt gctgaactcg
    17941 ccattcccac cgccgaatca cagcgggcac tggtcgggtc tggcattgaa gcgcaggtta
    18001 atggtgagcg cgtgttgata tgcgctgccg ggaaacatcc cgctgatgca tttgctggtt
    18061 tgattaatga actggaaagc gccgggcaaa cggttgtgct ggtagtacgt aatgatgacg
    18121 tgctgggtat cattgcattg caggataccc tgcgcgccga cgctacaact gccatcagtg
    18181 aactgaacgc gctgggggtt aaaggagtga tcctcaccgg cgataatcca cgcgcagcgg
    18241 cggcaattgc cggggagctg gggctggagt ttaaagcggg cctattgccg gaagataaag
    18301 tcaaagcggt gaccaagctg aatcaacatg cgcccctggc gatggtcggt gatggtatta
    18361 acgacgcgcc agcgatgaaa gctgccgcca tcgggattgc tatgggtagc ggcacagacg
    18421 tggcgctgga aaccgccgac gcagcattaa cccataacca cctgcgcggc ctggtgcaaa
    18481 tgattgaact ggcacgcgcc actcacgcca atatccgcca gaacatcacc attgcgctgg
    18541 gactgaaagg gatcttcctc gtcaccacgc ttttagggat gaccgggttg tggctggcag
    18601 tgctggcaga tacgggtgcg acggtgctgg tgacggcgaa tgcgttaagg ttgttgcgca
    18661 ggagataagg caaaccgatc gcaacattga gcgcgatcgg tcccctcgcc cctctgggga
    18721 gagggttagg gggtgaggaa aaggcggcat cgaagccaat cagcccctat caaccgcctt
    18781 tacgaatcaa ataacgataa ggcagtccat ccgtctcttt cgcaaccagt tcgtgttcca
    18841 taaaggtaca aaacccagga atatcgcggg tagtggccgg atcgtcggcg ataatcagca
    18901 acgtttcgcc aggctgcata ttgcgcacgg ttttgcgcac catcatcacc ggttccgggc
    18961 agcgcaggcc aagcgcgtcg agtgtgtggt cagggctgga aaagagatcg gtcattttct
    19021 tctcatcact taaaaaaacg gcgctagttt acgccctgtg agtccgtaag caaaccaggt
    19081 taacgattgc gtgaaaatta accattgcat tgtcaacgta aagcagtatc atgcggcggc
    19141 tcgaaaaaag ggtaagcacg ttattatgtt aaggtaacag acgtgtcgta cgtattgggt
    19201 tccctcaccc caatggttaa tcaaaaaggt acaatatgaa cgttttctcg caaactcaac
    19261 gctataaggc gttgttctgg ttatcgttat ttcatcttct ggtgatcacc tccagtaact
    19321 atctggttca gcttcccgtc tccatttttg gtttccatac cacctggggc gcgtttagct
    19381 ttccgtttat ttttcttgct accgacctga ccgtgcgtat ttttggcgca ccgctggccc
    19441 gacgcattat cttcgcggta atgatccctg cgttattaat ctcctacgtc atctcgtcgc
    19501 tattctatat gggttcctgg cagggattcg gcgcactcgc ccacttcaac ctgtttgtcg
    19561 cccgtatcgc caccgccagt ttcatggcct acgcgctggg gcaaatcctc gacgtgcacg
    19621 tttttaaccg cctgcgtcag agtcgccgct ggtggctggc accgacagcg tccacactgt
    19681 tcggtaacgt cagcgacacg ctggcctttt tcttcattgc cttctggcgt agcccggatg
    19741 cctttatggc tgaacactgg atggaaatcg cgctggtcga ttactgtttc aaagtgttaa
    19801 tcagtatcgt tttcttcctg ccaatgtatg gcgtattact caatatgctg ttgaaaagac
    19861 tggcagataa atccgaaatc aacgctttgc aggcgagtta aaggttcgtt atcagagttg
    19921 tgataagatg gatgaatgag ccgttatggc cgtttatcga aaggaagaag tcaatgcgca
    19981 atctggttaa atatgtcgga attggcctgc tggttatggg gcttgcggcc tgtgatgata
    20041 aagacactaa cgctacggcg cagggttcgg ttgcggaaag taacgctacc gggaatcccg
    20101 tcaacctgct tgatggcaag ttaagtttct cgctgccagc ggatatgacc gaccagagcg
    20161 gtaagctggg aacgcaggcc aataacatgc atgtctggtc cgacgccacc gggcagaaag
    20221 cagtcatcgt catcatgggc gatgatccga aagaagatct ggcggtgttg gcgaagcgtc
    20281 tggaagatca gcaacgtagc cgcgatccgc agctgcaagt ggtaaccaat aaagccattg
    20341 agctgaaagg tcacaaaatg cagcagttag acagtattat ctccgcgaaa ggccagacgg
    20401 cgtactcttc cgttattctg ggtaacgtgg gtaatcaact gctgaccatg caaattacgc
    20461 tgccagctga cgatcagcaa aaagcgcaaa ccaccgcaga aaacatcatt aatacgctgg
    20521 ttattcagta agttttaaga tgatgaggcg gcctcaggga cgtgttccgg aggccgtttt
    20581 tttaatcgcc acgtcagtaa taacgcgatt gcgaccagtc ccgccgccgc cagataaatc
    20641 accggtacgc ccgcccagct catcaccagc ccagccagtg gtccagtcac gccaagcgat
    20701 aaatccataa atacggtgta agttgccagc gccgccccct gattttgctg cggaaccgct
    20761 tttaccgcca ctacacccaa tgccgggaac accagcgaaa acccggcccc cgccagtaag
    20821 acgccgattt tcgccatcca cggcatagtc gccacgccaa ccagtagcag gccgattatc
    20881 tcaacgctaa agcaaatcat cgctacgttt aagccaccga tacggttaat gccgttaggg
    20941 aataacaaac gcgtaccgac aaacgcacag ctaaacagcg tcagcgcgaa agccgcaccg
    21001 tcccaacctt tagcgtcata aaacagcgtg ataaaggtgg cgatgacgcc aaatccggcg
    21061 gaagccagtg ccagcgccat accgtacagc cagacgcgcc caagcaccgc gcgaaacggc
    21121 agcggtttgc ctttactggc ttttaccgtc ggacgcggga tcgccaacaa aatggccacc
    21181 agcgccacgc ccataatgat taacgctaac gcctgcaagc cgccccagtg ataaaacacg
    21241 acgcctaacg gcgcacccat cgccatcgcc ccgtaagtga caatgccgtt ccacgaaatc
    21301 acccgcccga tatgcagcga gccaaccact ccaacgcccc atagggtcga tcccgttccg
    21361 gcaaaacttt gcccaatccc aaggatgacg cgccccaggc aaagtaataa caggctgatg
    21421 acaggcagac tggcggttaa tcctgccgtc agatacccca gaccgctcaa aaagcagccg
    21481 cataaaccga agacgacaat ctttttgggt cccagcaaat cggcgtaacg tccggcatga
    21541 gggcggctca gcaaggtggc gaaatattgc aggctgataa ccaatcctgc ccagaaggcg
    21601 ctaaagccca tcacatcatg gacatagccc ggtaatacag cgagcggcaa cccgatggtg
    21661 aggtagctgg cgaagttaaa catgacaatg gagacaatgc gcaaattcag gcgcaatccg
    21721 tttagcgcgg gttcggctac gggttcgggc atgaggatca ccacattttt acaacagtgt
    21781 ttcattttta ccatgtgctg acgtgaaaat cagcagtaag aatcagaata ttgctggcgt
    21841 gactcccgcg ctacacttaa cacaaaaagt cacaaggaag ccccaatgga aacccctcaa
    21901 cccgataaaa cgggcatgca cattctgctc aagttggcct cgctggtagt gatcctcgcg
    21961 ggcattcacg cagcggcaga tatcattgtg cagctgttac tggcgctgtt ttttgccatc
    22021 gtcctcaacc cgctcgttac ctggtttatt cgtcggggag tacaacgccc cgttgccatt
    22081 acgattgtag tggtggtgat gctgatcgca ctaaccgcgc tggtcggcgt actggcggca
    22141 tcgtttaacg aatttatctc tatgctgccg aagtttaata aggagctgac gcgcaaactt
    22201 tttaaattgc aggagatgtt gccttttctt aatttgcata tgtcgccgga gcgaatgctg
    22261 cagcggatgg actcggaaaa agtggttacc ttcaccacag cgctaatgac cgggctttcc
    22321 ggggcaatgg cgagcgtgct tttgctggtg atgaccgtag tttttatgct gtttgaagtg
    22381 cgccacgtcc cttacaaaat gcgttttgcg ctgaataacc cacagattca catcgcagga
    22441 ttacaccgcg cacttaaagg cgtttcgcat tatctggcgt taaagacgct actcagttta
    22501 tggacaggtg tcatcgtctg gctggggctg gcgctaatgg gcgtacagtt tgcgctgatg
    22561 tgggcagtac tggcgttttt gctcaactac gtgcccaata tcggcgcggt aatttccgcc
    22621 gtaccgccaa tgattcaggt gctgctgttt aatggtgttt acgaatgtat tctggtcggc
    22681 gcattgtttt tagtggtcca tatggtcatc ggcaatattt tagaaccacg gatgatgggc
    22741 catcgcctgg ggatgtccac catggtggta tttctttcat tgttaatctg gggatggctg
    22801 ctcggcccgg tagggatgct actttcggtg ccattaacca gcgtgtgtaa aatctggatg
    22861 gaaacgacga aaggtggcag caaactggcg attttactgg ggccgggcag accgaaaagt
    22921 cggttaccgg gatgaggcga caagtgatac gatacgcact ttcattttcc attaaacgtt
    22981 ggccctgata tgtatcggat agttctgggg aaagtttcga ccttaagcgc agctccactg
    23041 ccaccgggtt tacgcgagca agcaccgcaa ggtccacgac gcgaacgctg gctggcgggg
    23101 cgtacattgc tttcgcacac gctttccccg ctaccggaga tcatctatgg cgaacaaggc
    23161 aaacctgcat tcgcgccgga aacgccgcta tggttcaact taagccatag cggtgacgat
    23221 atcgctctgc tgttgagcga tgaaggcgaa gtcggctgcg atatcgaagt gattcgcccg
    23281 cgcgtcaact ggcgctggct ggcgaacgcc gtgttcagcc tcggggaaca cgctgaaatg
    23341 gacgccgtgc atcctgatca gcaactggaa atgttctggc gcatctggac gcgcaaagaa
    23401 gccatcgtta aacagcgtgg cggcagcgcc tggcaaatcg tcagcgtaga tagcacctat
    23461 cactcctcgc tgtcagtcag ccattgtcag ctagaaaatt taagccttgc gatctgcacc
    23521 cccaccccct ttacgctcac cgccgacagt gtgcaatgga tcgattcagt taactgatcc
    23581 gcccacccga ctgcccatct attgatccag aacaggtaat cagtatgacg aatacttaaa
    23641 atcgtcatac ttatttccgc catctatttt aatccattgg ggttaccatg ctctccacac
    23701 tccgccgcac tctatttgcg ctgctggctt gtgcgtcttt tatcgtccat gccgctgcac
    23761 cagatgaaat caccaccgcc tggccggtga atgtcgggcc actaaacccg cacctttaca
    23821 cgcctaacca gatgttcgcc cagagcatgg tttatgaacc attggtgaaa tatcaggcag
    23881 acggttcggt gatcccgtgg ctggcaaaaa gctggactca ttcagaagat ggtaaaacct
    23941 ggaccttcac cctgcgtgat gacgtgaaat tctccaacgg tgaaccgttc gatgccgagg
    24001 cggcggcaga aaacttccgc gcagtgctcg ataaccgtca acgtcacgcc tggctggagc
    24061 tggcaaacca gattgttgat gttaaagcac tcaataaaac agagctgcaa attaccctga
    24121 aaagcgccta ctatcctttc ctgcaagaac tggccctgcc ccgtcctttc cgttttatcg
    24181 ctccctcgca gtttaaaaac catgaaacca tgaacggaat taaagcgccg attggcaccg
    24241 gaccgtggat tttgcaggaa tcgaaactga atcagtacga tgtcttcgtc cgtaacgaaa
    24301 actactgggg cgaaaagcca gcgattaaaa agatcacctt taacgtcatc ccggacccga
    24361 ctacccgcgc ggtggcgttt gaaactggcg atatcgacct gctgtacgga aacgaagggt
    24421 tattaccgct cgataccttc gcccgcttta gccagaatcc ggcttaccac acccaactgt
    24481 cacagccgat cgaaaccgtg atgctggcgc tcaataccgc caaagccccc accaacgagc
    24541 tggcagtacg tgaagctctt aattacgcgg taaacaaaaa atcgctgatt gataacgcgt
    24601 tatatggcac ccagcaggtc gccgacaccc tgtttgcccc ttctgtgcct tacgctaatc
    24661 tcggcctgaa accgcgccag tacgatccgc agaaagcgaa agagttgctg gaaaaagccg
    24721 gttggacgct gcctgcgggc aaagacatcc gcgagaaaaa tggtcagccg ctgcgcattg
    24781 aactgtcgtt catcggcacc gatgcgttaa gcaaatcgat ggcggaaatc attcaggctg
    24841 atatgcgcca gattggcgca gatgtctcgc tgattggcga agaagagagc agtatctatg
    24901 ctcgtcagcg cgacggtcgt tttggcatga ttttccaccg cacctggggc gcgccatacg
    24961 atccacacgc cttcctgagt tcaatgcgcg taccgtcgca cgctgacttc caggcacagc
    25021 aaggattagc cgacaagccg ctgattgata aagagatcgg cgaagtgctg gcgacccatg
    25081 acgaaacgca acgtcaggcg ctgtatcgcg acattctgac ccgtctgcat gacgaggcgg
    25141 tttatctgcc tatcagctac atctcgatga tggtggtatc aaaaccggag ctgggtaata
    25201 tcccctacgc gccgatcgcc accgaaattc cgttcgaaca gattaaaccg gtgaaacctt
    25261 aatgttgcgt tacgtattac gccgctttct gctgctgatc ccgatggtgc tcgccgcctc
    25321 ggtgatcatt tttctgatgc tgcgcctcgg taccggcgac ccggcgctcg attatctgcg
    25381 tttatctaac ctgccgccga cgccggagat gctggcctct acccgcacca tgctcgggct
    25441 ggatcagccg ctgtacgtcc agtacggtac ctggttgtgg aaggcgctgc atcttgactt
    25501 tggtatctca ttcgccagcc aacgcccggt actggacgat atgctgaact tcctgcccgc
    25561 cacgctggaa cttgcaggtg cggcgctggt attaattctg ctcacttccg taccgctcgg
    25621 tatctgggcg gcgcgccacc gcgatcgttt gccggatttc gccgtgcgct ttatcgcgtt
    25681 tcttggcgtg tcgatgccca acttctggct ggcatttttg ctagtgatgg cgttttcggt
    25741 gtatctgcaa tggctacccg cgatgggcta cggcagctgg cagcacatca ttttacctgc
    25801 ggtttccatc gcctttatgt cgctggcgat taacgcgcga ctgctgcgcg ccagtatgct
    25861 ggacgtcgcc ggtcagcgtc acgtcacctg ggcgcggcta cgcggcctga atgacaaaca
    25921 gaccgaacgc cgccatattc tgcgcaatgc ctcgctgccg atgatcaccg ccgtggggat
    25981 gcacattggc gaattgattg gcgggacgat gattatcgaa aacatctttg cctggcctgg
    26041 cgtcgggcgc tatgcggtgt cggcgatttt taaccgtgac tatccggtga ttcagtgctt
    26101 tacgctgatg atggtggtgg tttttgtggt ctgtaatttg attgtcgatt tgctcaacgc
    26161 cgcgctggac ccgcgcattc gtcgtcatga aggagcgcac gcgtgaactt tttcctctct
    26221 tcccgctggt cggtacgcct ggcgctgatc attatcgccc tgctggcgct gattgcgctc
    26281 accagccagt ggtggcagcc gtacgatcca caggcaattg atttgccgtc gtgcctgctt
    26341 tcgccggatg cgcagcactg gctgggcacc gatcacttag gtcgcgatat tttctcgcgg
    26401 ctgatggcag cgacccgcgt gtcgctcggt tcggtaatgg cctgcctgct gctggtgctg
    26461 acattagggc tggttattgg cggcagcgcc gggttgattg gcgggcacgt tgatcaggcc
    26521 accatgcgcg tcgccgatat gtttatgacc ttcccgacct cgattctgtc gttctttatg
    26581 gttggcgtgc tcggcaccgg gctgaccaac gtaattatcg ccatcgccct gtcgcactgg
    26641 gcgtggtatg cacgcatggt gcgcagcttg gtgatttcac tacgccaacg cgagtttgtg
    26701 ctggcgtcac ggctttccgg tgcgggccat gtgcgggtgt ttgtcgatca tctggcaggc
    26761 gcggtgatcc cttcgctgct ggtgctggca acgctggata tcggccatat gatgctgcac
    26821 gtcgcgggga tgtctttcct tggcctcggt gtgaccgcgc cgaccgccga atggggcgtg
    26881 atgattaacg acgcgcgcca gtatatctgg acccagccgc tgcaaatgtt ctggccgggg
    26941 ctggcgctgt ttatcagcgt gatggccttt aacctggtgg gtgacgcact gcgcgatcat
    27001 ctggatcctc atctggtgac ggagcacgca cactaatgcc gcaacagatt gaactacgta
    27061 atatcacgct acaggccgcg cagccgctgg tacacggtgt atcgttaacc ctgcaacgcg
    27121 ggcgcgtgct ggcgttagtc ggcggtagcg gcagcgggaa atcattaacc tgcgccgcga
    27181 cgctgggcat tttgcccgct ggcgttcgcc agacggcggg ggaaatttta gccgatggca
    27241 aaccggtttc gccttacgcc ctgcgcggca tcaaaattgc caccatcatg cagaacccgc
    27301 gcagcgcctt taatccactg cacaccatgc acacccacgc gcgggaaacc tgcctggcgt
    27361 tagggaaacc cgccgatgac gctacgctta ccgctgccat agaagcggtg gggctggaaa
    27421 acgccgcgcg cgtgctgaag ctgtacccgt tcgagatgag cggcggcatg ttgcagcgca
    27481 tgatgattgc gatggcggtg ctgtgtgaat caccgtttat catcgccgat gaaccgacca
    27541 ccgacctcga cgtggtagca caggcgcgca tcctcgatct gctggaaagc attatgcaaa
    27601 aacaagcgcc gggaatgctg ctggtcaccc atgatatggg cgttgtggcg cgtctggcgg
    27661 atgacgtggc ggtgatgtca caaggtaaaa ttgtcgaaca gggcgatgta gaaacgctgt
    27721 ttaacgcccc caaacatacg gtgacgcgca gcctggtttc cgctcatctc gccctctacg
    27781 gtatggagct ggcatcatga ccttacttaa cgtctgcggc ctttcccatc actatgccca
    27841 cggtggattt agcggaaaac atcaacatca ggcggtgctg aataacgttt ccctgaccct
    27901 gaaaagcggc gaaaccgtcg ccctgctggg acgcagcggc tgcggaaaaa gcaccctcgc
    27961 gcggctgctg gtaggcttag agtctccctc acaagggaat attagctggc acggcgaatc
    28021 gctggcgaaa cttaatcgcg cccaacgtaa agccttccgc cgtgatattc agatggtgtt
    28081 tcaggactcc atcagtgccg tgaatccgcg caaaaccgtg cgcgagatcc tgcgtgaacc
    28141 gatgcgccac ctgctatcac tgaaaaaatc cgaacaactg gcgcgcgcca gcgaaatgct
    28201 gaaggcggtc gatctcgatg acagcgttct cgacaaacgc ccaccgcagt taagcggcgg
    28261 ccagctccag ctcgtctgcc tggcacgcgc gctggcggtc gaaccgaaat tgctgattct
    28321 ggatgaagcc gtttctaacc ttgatctcgt gttacaggcg ggcgtcattc gcctgctgaa
    28381 aaagctacaa caacagtttg gcactgcctg cctgttcatc actcatgact tacgcctggt
    28441 ggaacgcttt tgccagcgag tgatggtgat ggacaacgga caaatcgccg aaacccaggc
    28501 ggtgggagat aaattaacct tttcctctga cgccggacgt gtgctacaaa acgcggtatt
    28561 acccgcattc cccgtgcgcc gtcgcacctc agaaaaggtt taacgccaat gcaacgagtc
    28621 accatcacgc ttgatgacga tttactggag acgctggaca gcctgagcca gcgtcgtggt
    28681 tataacaacc gttccgaagc tatccgcgat attctgcgta gtgccctggc gcaggaagct
    28741 acccagcagc acggcacaca aggtttcgcg gtgctgtcgt atgtgtatga acacgaaaaa
    28801 cgcgacttag ccagccgtat tgtttccacc cagcatcatc accacgacct ctccgtcgcc
    28861 acgctacatg tgcacatcaa ccacgacgac tgtctggaaa tcgccgtgtt gaaaggtgac
    28921 atgggtgacg tgcagcattt tgccgatgac gttatcgccc agcgcggcgt gcggcacggg
    28981 catttgcagt gcctgccgaa ggaagattga gttatttcta aaacaaggaa agaaaagata
    29041 ttaactgaac ttaatttata aaattaagtt cagttgcata gatagatcct gtaaaggcag
    29101 atattctgcg taatgtacgt cgctaaaatt tataaatgat tcaaacgctc attcagtgtt
    29161 tcaataatat aggtattaac agagacttgt tgctgcgcag ccgcattatt aaggcgttca
    29221 ctcagcgatt ctggatagcg taacgtaaag gtcttaatct tttctgtacg cgcatacggc
    29281 tcaatacccg cagctttaca atcctcaaga tactctcgca gggaaagctc tccttctttt
    29341 tgcagcccct gaatactgtc agagacaaaa tcacaataac ctgacaagcc caggaatttg
    29401 ccacgaaatg cattcagttc tggcacgtag gtaataacag ctggttgccc ggcaatctcc
    29461 atagagtttg gcgtcttcag tttgatcatg gttttacccc tatacttagt aaccagtcac
    29521 acacgctttc tacagcgcct ttatcggtat ctggcgacgg atgaggccga tggaaacacg
    29581 caacgctcat atttagtatg aacttgcaac gcgaacctct tccttcctta atttctccgc
    29641 ctaatgcttt aaccagtgac tctatatcag accacttgat cccttgagga acaggagttt
    29701 taaatatttg atccaatgta tttttttgtt tcttccgtaa agcctgtacc ttggttctca
    29761 tggcatgtcc ttatgacttc actttatgac atcacagtat gaagtcaagt ttcccttcct
    29821 tacgccattg tcccaatcgt cttcctgaat cgcagcagtg cgatggtgaa aaacgcgccg
    29881 ccaattgcca tcagcgtcag gaactgcggc cagacgattt cgaatccggc accccggtag
    29941 aggatggcct gcgcgaggct aacaaagtgt gtcgtcggca tggtcagcat aatgtcctgc
    30001 accatctgcg gcatactttc gcgcggcgtg gatccaccgg aaagcatttg cagcggcagc
    30061 agcaccagaa tcaccagcag ccccagttgc ggcattgaac gcgctatcgt ccccataaaa
    30121 atgccgattg acgtggtggc aaacagactg agcgccacgc ccagcataaa cagcgggatc
    30181 gagccttcaa tcggtacgcc cagtacacct ttcaccatca gcaccagcga taatcccgat
    30241 accaccagca ccaccagccc catcgaccag atcttcgcca tcatgatctc aaacggcgtt
    30301 atcggcatca ccagtaaatg ttccaccgtg ccgtgttcac gctcgcggat cagcgccgat
    30361 ccggtcaata caatcgccag catggtaatg ttgttgatga tcgccatcac cccgccaaac
    30421 cacgcgggat caaggttcgg gttaaagcgc atccgggttt ccagcgatac caacggttcg
    30481 ctgttatcac ggtagcgcgc gacaaagctg ttcacttcac cgttgataat attctggata
    30541 tacccattgc cggtaaatgc ctggctcatg cgcgtggcat cgacgttcac ctgaatatcc
    30601 ggctggcgtc cggcgaggac atcacgctga aaattaggcg gaatatttat cgcgaaggta
    30661 tagcgtccgg cgtccagtcc ggcatccatc tcatcggcgg tgatcatctc cggtggcaaa
    30721 aaccacggac gatagaagct gttaacgatc cggttcgata actgcgattg atccatatcg
    30781 gcaatggcga tcggcgcgag gttcaacgat cctggcgtaa cggtcgctga cgaatacacc
    30841 gacaccgtaa acgagaagac aatcagcgtc agcatcgctt tatcaccgag cagactgcgc
    30901 aactctttga tacccagatt aaaaatattg cgtaaatggc gcatcatccc tcctgttttt
    30961 tcagcagcag gatgcttaag cccatcacca gcgggatggc tatcagcaac gggataaaaa
    31021 gttgccacaa atcagtcaga tccagcgctt tcgagaacgt tccgcgggcg atagtcagaa
    31081 aatgactggt cgggtaaacc tcgccgatcc aacgtccagg cccttccagc gaagctaccg
    31141 gatcgatcat cccggaaaac tgtgtcgccg ggatcaatgt gataatcgcc gttccgaaaa
    31201 tggcggcaat ctggcttttc ataaaggtgg agatcagcag ccccattcca gtggcaatga
    31261 tgatatacag cagcgccgcc aaggtgagcg tcaggaaact gcctttatgc ggtacgccaa
    31321 acacaaacac cgacaggccg cagagcagga aaaagttcag catccccagc gcgatgtacg
    31381 gcagctgttt gccaagcaaa aattcgctac gggtggtggg ggtcacgtaa aggttgataa
    31441 tcgaccctag ctctttttcc cgcaccacgc taagggcgct tagcattgac gggatcatca
    31501 tcagcagaag cgggatcacc gccggaacaa tcgctggcag gctttttacg tccgggttat
    31561 agcgataacg cgtctcaatg ttcatcagcc cgctttggct ggcgggtgtc gattgtcggc
    31621 tcgccacatc ctgaagccaa ctctggtgca ttgcctgcac gtaacctttt accgtttcgg
    31681 cacgactggg catcgctccg tcgatccaga cgccgagttc cacaggcgta ccacgcgcga
    31741 tatcgcgccc gaaattgggc gggatctcaa tggccaccgt gatatcgccc gcacgcatcc
    31801 gacgatcaag ctcgtcataa ctggtgagcg gcggctgttc gataaagtaa cgggaaccgg
    31861 agaggttgag tgtccacgcc tggctactga cggtctggtc gcggtcgagc accgcaaagc
    31921 gcaggttttc cacatccata ctgatgccgt aacccattat cagcatcagg atcaccgttc
    31981 ccatcagcgc cagcgtcgaa cgtactggat cgcgtcgcag ttccagcgct tcgcggcggc
    32041 tgtagctaaa cagacggcgc aggctaaatc cctgacgcgg cgcgtgggtg gtgtcgtgta
    32101 ccacgggcgg cgcttcggct tcgttgctct gccctgccgc ttcctgcaaa taggcgataa
    32161 atgcctcttc cagactggcg gctccgcgtt tctcaaccag ttcctgcggt gtaccgctgg
    32221 caagcacttt tccggcgtgc atcagtgaga tgcggtcgca acgttccgct tcgttcataa
    32281 agtgggtgga gatgaagata gtcactttgt cctggcgcga gagatcgacc atcaactgcc
    32341 agaacatatc cctcgccacc ggatcgacac cagaagtagg ctcatcgagg attaacatct
    32401 ccgggcgatg aatcaccgcc accgccagcg aaagccgctg gcgaatgccg agcggcaatg
    32461 actccggcag aacatcttca acgtcgttga gcttaaaacg ctcgctcatt tcagccactc
    32521 gcgcgggaat ttccgcttcc gggatgtgaa acaaacgggc atgtaactcc aggttttgcc
    32581 gcacggtgag ttcgttatag agcgaaaacg cctgcgacat atagcccaca cgacggcggg
    32641 tatcgatatc ttttggatca accggttgcc caaatagcca cgcctcacct tcgctggcgg
    32701 gcagcagtcc ggtaagcatt ttcatggtgg tggatttacc gcagccgttc gaaccaagga
    32761 atccaaaaat ctccccgcgt ggaatgcgga aattgacgtg atcaacggca acgaaggaac
    32821 caaaacgcat ggtcagatcg cgcgcttcga tggcaatctc tgcgttttca ggttgatacg
    32881 gtgggatcac taccgcctga tgcgcctggc gttgcgcttg cggtaacaga ttaataaatg
    32941 cttcttccag cgtggcgctt tgcgtttgct gccgcagctc ttcggcgctg ccggttgcca
    33001 gcacttctcc ggcattcatc gctaccagcc agtcgaagcg ttcggcctct tccatatagg
    33061 cggtggcgac cagcacgctc atattgctct gccgctggcg aatactgtcg atcagatccc
    33121 agaactgggc gcgggagagc gggtcaaccc ccgttgttgg ctcatcaagg atcaacagtt
    33181 ccgggtcgtg gattaacgcg cagcacagcc caagtttttg cttcatcccg ccggagagtt
    33241 tacctgccgg acgatcgcga aacggtgcta acccggtgct ggtcagcagc tcattaattc
    33301 gcgcttcccg ctccgcttta tcgtgaccga acaggcgagc gaaaaaatcg acattttcat
    33361 acaccgacaa ggtgtggtag aggtttttgc ccagcccctg cggcatccag gcgatgcgcg
    33421 ggcagacgtc tcggcgatgc ttcgggtcgt gcatatcgcc acccagcacc accacattgc
    33481 cctgctcaat gacgcgggca ccggaaatca gtgacaataa actcgacttc ccgacgccat
    33541 ccggaccaat cagcccgacc atgcaacggg ccggaatatc gagagtgata ttgttcagcg
    33601 caacggtttt tccataatgc tggctcacgc ccgccagttg cgcgacaggc gggacgggaa
    33661 ccagttccag atgcgtcatt gcggcaacct caccacgagg tcgtcaggcc acggaagttc
    33721 ttcattcacc cgcacccacg ctacgcccgg taaaccggtt ttgacatatt ccagatgctg
    33781 ctggagtaat tccggtggga tacgcgcttt gacgcggaac atcagtttca gccgttcatc
    33841 gctggtttcg acggtttttg gcgtgaactg ggcgacactg gcgacaaaac tgatggttgc
    33901 aggaatacgc agatctggcg cggcatcgag gatcagccgg gcttcaccgc ccagtttcag
    33961 cgtgcccgcc tgttcggttg gcaggaagaa ggtcatatag acgtcgctga gatcgaccat
    34021 attcagcacc cgaccgcctg ccgccagcac ttcccctggc tcggcaaccc gatactgcac
    34081 gcgtccgtca cgcggggctt tcagttcact gtcatcgata tctgcggcaa tgcgccgttc
    34141 agtggcttgc gctgcttcga cccgagtttg cgcctgaatg atattggtgc gtgctgcttc
    34201 tatagccgct ttagaagccg aaacctgggc tttcgctgat tccagcgcag ctcgggcgct
    34261 ctcagcggcg gcgcggtcgt catccagctg ttgcgcagaa atagcccctc gttgggcgag
    34321 cgagcgggaa cgcgtatgac gtttggcaac agagtccagt tcggcctggc gttgattgac
    34381 cagcgactgt gcggcacgag tttcactttg tcgttgctcc agcaaagcct gcgcggcggc
    34441 aacggcgctt tgtgcctctt tgatttgcgc gatggcttcc agtcgctgtt cctgcaacac
    34501 gcgagtatcc atcttcgcca gcacttcgcc ttcgcgaaca aactggcctt ctttcaccag
    34561 aatggtgtcg atacgcccgg caattttgct ggcgatatcc acttccgtcg cttcaatgcg
    34621 cccattgctg acagcaaagc cttccggcac acccgccggg cgcaacagcc accacgccac
    34681 gacggcagcc accgccagtg ccccgacaac ccaccacgcc agatggcgct tactcttatc
    34741 cataatcgac ccgccataat ccctgtaagc aaacgacgcg tgttacaact gccgccgaac
    34801 gacgttcagc ggattcttcc atccgggata agaaggaaac gacaaatacg accagcgcgc
    34861 agagcattga gcgccaacgg ggaaggctta gtgaaaacgg gtagtaatgt tgtcatcatc
    34921 agcattcctg gccgtaaatg aaacacgcat actcataagt gtgatctata aaactggctg
    34981 acaaaacggt atccatcctg ttggatcgca tttcacgcct gttcagttgc aggaacagag
    35041 cattcctttt tacgttttat caacagtctc aaccagatag taacattaat attcatgttg
    35101 tctatggttc agagctgtaa cattgtgcgc cttctaatca aaggcataag tccatttctg
    35161 tttttttcat gctggtaaga cttccaggga gtggttatct ctggccctgc accagggtta
    35221 atttggcaat tatgcatgct gaacattctt ccagcatgtt tactgtcacg ggcgttaaga
    35281 cctaaaactg tccccgtttc acctatgcgt tctctcccac catttccgtt atgtgcaaca
    35341 acttgattta tacctaacat ttgtgaacat gctaatagtg cttcaagttt aggtctgtaa
    35401 tctgtcgaaa cagaaaaata acgattaaag gatcgacaag agatagcccg gctagtttta
    35461 aactttttgc cattaattat agcatgatgc tttttattaa taatttccgc taattcgacc
    35521 ggatcgagag cattggtcat ttcatcaata gagacagtta tcggtcctat aatgtaacgt
    35581 tttcgctgat catcaaaagt tatcccatga tgatttgcca ttattttgta tttggggtca
    35641 tagaaacata caggataatc ttttatcccg tcgtaggtat cctcatcaag tggtttcatg
    35701 cgcgtgaagt tttgcatata gttaccattg caattcgttt catgatttcc agctaataca
    35761 tgaacatttt cattgatact cattatgcgt tttaacaaat cgatgataaa ttgatcgcca
    35821 cagatattac taaatcgatc gccggtttgg tcgccaagaa acgtacatgg cgtgactgcg
    35881 tctttattaa taaccaaata aggctgaaga ctttctaaaa cattatctct tctgttcagt
    35941 atactgatta ttgtattaac attagtgtca agttgctcat taaagttctc ccttagcaat
    36001 acgttgagaa cttcatcttc tatattaagc aactggctaa ggaaattttc tccttcagcc
    36061 atgattccca aatgtcctgt tgcaaaaagc gcgtacaaaa ctgcaccgac tgagccatcg
    36121 gtatcgccaa aataggtcgg cctggtactt gtgggatcaa caggtgaaca attggagaaa
    36181 taggttctgt cagcgaactg gggttttata attgtttcga atttaatatc aaatacattg
    36241 tattgcgata tttcccagtt ccagatgggt ttgtacgact cagtaactgc aacatcatta
    36301 gcattatcat ttactgtcat attattaagt aaataaacaa tgctgcacca attaatatcg
    36361 gtattatcaa gagggcaacc gtaattttta ctattaattg tcacaataaa ctgataacag
    36421 ttattcctac gtagaatatg agcgcgcatg cccgatagca tactatattt ttctgaatga
    36481 ttcgtttgta tggtaaatga aattttgtca tcttgaatag attgttggaa acgttctatt
    36541 tgccaatcat caattttttc cacaacttca ccgagcattg tcctggcaat acttgatata
    36601 tcatcaccta taccatttgc aatataccca aacagttctt tagtgaagtt ggtaacatgc
    36661 tcttgcacca tatcatttgt tgcgattgtc gttgtactgc cattagtgcc tgccacagtt
    36721 ccaattttca tcttttacct catattattc accgcgagga atacatagtt aacattttat
    36781 cttatgacat acacctcata ttcgatgggg aaataattat attttgcaca aatcattcag
    36841 ccttaaagca taatatgaaa tttcttatct catagcagag aaagtcccta aatattgtag
    36901 aggcgtcatc agtacatacc tctacaatcg attaagaaaa cctatgaaat actggcaaaa
    36961 ttaaaaagag ataccacagc actcatgaag ttttattgag gttgaaaaca actaagccct
    37021 gggacacctt ttttatcacc acgttcgtta ggtgctatct tccaagaatt tagccccatg
    37081 gtataattat caattttttg atgctggtct acccctaatc cataatggcc aaatttctgt
    37141 ttagtttttt tgagcgtctg tccacatttg aagatatctt caggttttgg tctgaaagca
    37201 gtagtgttat taaaataatt ttcatataca ggtgtgggtg ctatagcatt gcttctaatt
    37261 agatggagaa gatgatcatc catgtgttgt ttatgttttt tattacatat atttgctaat
    37321 tcctcaggat caattgggtc tttcatctga ttaaatggaa cctgcaaagc accgagacaa
    37381 taacattgat tttcttcatt tcttattatt ccgtgatgac tggttaaaac tttagtttct
    37441 gagttataat tacaaacatc gagcgtttta attaagttat acgtatcacc ggcagagagt
    37501 ttataatttt ctagtcgtgc agtatagtta ccattaaaat tgatttcatg atttccagcc
    37561 agaacgatga catttttatt aatacggcta tctttattac cctccatatt tcgcatagaa
    37621 tttagcaaag taaggatata tttatcacca aaaatagtac taaatctatc gcctgtatga
    37681 tccccaagga agatacaagg ggttatcgca tcgtccttta aaaaaacatg ttgatgcaac
    37741 tcattcaata tcatgtctct cgtatttaat aaattgtaga gcaatttatt atttttatgt
    37801 atgtatgcag atgaagccat gtcttcgtgt tttaataatt cacataataa actccaaccc
    37861 ttttccctga tgcctatatg ccctgaaacg agcaatgaat aaagtacagc gcctactctt
    37921 ccatctgtat cgccaaaata agccgtgttg gcattttgtg ggttaatcac accacattgt
    37981 ttataaaagt ctattttaga aaacacagat ttaacatagc tatcaaactt atccttagga
    38041 aacgtctctt tagagacatc ccaacgccag tcagctttaa aattaccgct aagaagagta
    38101 aagaaatttt caggtatgat agaaagccca ttaactttat aaattatgtc tgatttatct
    38161 ccggagaaat taataaaata ataaccatcc ttacactcaa tcttaactgt aatgggagac
    38221 gataatttta tcacatggtt attgtctggt ataattgtaa agataaacgt gttagtagta
    38281 ttatcggcaa aactattata ttcttgaata tctatagatg gctcactatt gtaaaaccat
    38341 attttctcca tctgaagact atgttcattt accctccagt gaccatgttg caggaaatag
    38401 atgatatgca tgccaaaatc gttatctatt tgcgaatcat tatccagctt atcaatttca
    38461 actatctttg aacgatgtgt tttatcaacc ttgctatggt gttgatgatt attaacttta
    38521 tctgaatgct tatctttatg gtttgaaggg atgtacttaa atatattgtc aattgctttc
    38581 ataaataacc cgcgatattt ttgacctgtt agtaaaaaat aaatgataca tgtctgaaag
    38641 acatatttac gacgatataa aacaataata cttatatgtt cgctgatttt aagcaccgag
    38701 actatccggt cttttactaa ataatggaaa acaatccatc cctggaagac aattgtaaaa
    38761 aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg gccttttgac
    38821 cgctaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctatg catttgtctt
    38881 taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac atttcaatat
    38941 tagcagggat acctgaggag tttaaagtga acatatatat cgggtagctt ttcaaattaa
    39001 tccctttgat tataggctta atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag
    39061 ggcaacgtca cgggtcatgt gctgatttct ctggcggcca tatgcctggc attattcact
    39121 accgcattta ttatcatttc gcaactcacg cgcggcgtta atacgtttta caatacattg
    39181 ttccccatta ttggctatgc ggggtcaatt atcaccatga tatggggttg ggcactgtta
    39241 gcaggtaatg atgtgatggc agacgagttt gttgccggcc atgttatttt tggcgttggt
    39301 atgattgccg catgtgtatc gacggtggca gcgtcgtccg gtcactttct gctcattccc
    39361 aaaaatgcag cggggagcaa gagcgacgga acaccggtac aggcttattc ttcattaatc
    39421 ggtaattgcc tcattgccgt tcccgtttta ctcaccctgc tcggtttcat ttggtctatt
    39481 acgctgttac atagtgccga cataactccg cattatgtcg ccttgggtta accgcaatct
    39541 gtgcctgtct aattggccta gtggccacaa tcgtccatca aacacgtaat acgttctcat
    39601 ccaaagagca ctggctgtgg tgttattggg ttatttttct cggctcagtc acggtgctgc
    39661 aggggatata cgtcttagtc agttccgatg caagcgcccg actggctccc ggcattattc
    39721 tcatttgcct cggaatgatc tgttacagca tattctcaaa agtctggcta ctggcactgg
    39781 tctggagacg tacctgttcg ttagccaaca gaataccgat gataccggta tttacctgtc
    39841 tgttttgctt gtttttgtca tcattcctcg cggaaatcgc acaaactgag ctggactact
    39901 ttattccatc tcgggttctg gttggattag gcgcagtatg ttttacattg ttctctattg
    39961 tttcaatatt agaggccggt tctgcgaaaa aaaattaaaa tttacataat cccagcgtta
    40021 ttcatatctt aacgctggtt ttttacgaac ttagtgaaat aatgtagaat ataaaattat
    40081 aattttatat tggagcacat gtgttaatga tcaaccccgg gatactaaaa atatagacat
    40141 tattaaactt gcttagcctc gcattgcctt tacctgttaa agatgcatcg agaaaattga
    40201 gtctgaagtt ctcatccttt atctgatttt ccagtcccga aactttggcc ttagcattac
    40261 ctgagatccg tccgttacta tctattttgt aggtaaaaaa atacgaatgg gaaaatttcc
    40321 tcggtgtatc ttcatcagtt aagccaacaa tcgtaaaact accgctacca tcttttttta
    40381 aaatgactga aaggttagca gagtagtata aagaatgatc tgttttagca tttcttgtta
    40441 acgtcgacgt acattcaaga gacgttgtgc taccatcacg agccttccag agagcaaaaa
    40501 aacacacctg tagcaatgtg aaaacaagta ttaatataaa atacattttc ttattagtca
    40561 tataatagca cctctgaaca aattgcataa gtttcgtcat tagaggattt tatatatctt
    40621 gataaatacc gctgcccttc cccaagcaaa taatgcatac catttacatc atatataaaa
    40681 tttgcacctg gcacacaggg taaatgcttt ttcgaaattt cagcggcctc tatttgagat
    40741 ttagcgaggc caatctcgtt aagattctct gaaagatata aatcacaggt gtcaatctta
    40801 cctgatttaa ccaatggata atccgttata ctgtaggtat ctttaaaagc cacggcaaac
    40861 atttcaataa taacaattac taccacaagc gaaatcaaaa tgcgaaaaag aggaatactc
    40921 cttttgcgct ctactttttc aacaacatct tcttgtaaat gagaatcatc ataagattgt
    40981 ttacttattg ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca
    41041 ttttcttcaa caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat
    41101 gtgttgtttg acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg
    41161 tcaaccgctc caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctgga
    41221 aaatttatct catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata
    41281 atcgcgtcat tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag
    41341 attttgtcat tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata
    41401 accctgaaaa agtcatataa aacttatatc cacagaataa aaaatcaaac atgtcagctt
    41461 tatcaatata aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa
    41521 gcgcaccaca tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac
    41581 tcaggagcta cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat
    41641 gatacctgat gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag
    41701 agtgacagag tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt
    41761 tgaactcatc tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa
    41821 aagagactta aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga
    41881 cgtgttaatt atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt
    41941 tgaagaaaat ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga
    42001 agatctttgt tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa
    42061 gattgcaaaa atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag
    42121 atttttaaca gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg
    42181 tagatccgtt aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca
    42241 taaagttcgt gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta
    42301 taaagcgcta attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt
    42361 ctatgtcttt ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact
    42421 aatcatctgc attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc
    42481 tcaacaacct gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt
    42541 ttcagcatca tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc
    42601 tcaagcgttg tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact
    42661 gcatccgctg aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg
    42721 tggctctgcc catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac
    42781 gtaccgctat ataccccttt cactggttga atggtattat taacaacgat aggcttggtc
    42841 aaagtaagat tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca
    42901 aaagctgtcc ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga
    42961 ttttcattta cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat
    43021 gcttccacca aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga
    43081 aactataaac atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt
    43141 tggtattttg cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct
    43201 tccgcactca agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt
    43261 tcagtgtctt tgctataact aatcacagcg ccgaagggga tgggtttacc ctctttctgt
    43321 tttaaggtta ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct
    43381 ccagctgtcg gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt
    43441 ttttcatcca gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca
    43501 cgaaaatcag tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc
    43561 tctataaggg ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata
    43621 agtatcccac cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg
    43681 ctactaaact gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc
    43741 ggttgtgact gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta
    43801 ttctgacctg ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt
    43861 gaagacgcat aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt
    43921 aatttccgat ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg
    43981 gagaatgtta tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct
    44041 gtgccccggg aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg
    44101 ggctgggata aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt
    44161 tcatcctgtg cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg
    44221 gttataga
//
LOCUS       DAJTMX010000018        42870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTMX010000018 DAJTMX010000000
VERSION     DAJTMX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.018"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO145_001004"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO145_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5466206.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO145_001005"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO145_001005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5466207.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO145_001006"
     CDS             complement(3736..4092)
                     /locus_tag="OO145_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5466208.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO145_001007"
     CDS             complement(4096..4512)
                     /locus_tag="OO145_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5466209.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO145_001008"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO145_001008"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5466210.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO145_001009"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO145_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO145_001010"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO145_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5466211.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO145_001011"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO145_001011"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5466212.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO145_001012"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO145_001012"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5466213.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO145_001013"
     CDS             10521..11504
                     /locus_tag="OO145_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5466214.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO145_001014"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO145_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5466215.1"
                     /translation="MLELLFVIGFFVMLMITGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO145_001015"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO145_001015"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5466216.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO145_001016"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO145_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO145_001017"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO145_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5466217.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO145_001018"
     CDS             complement(15306..15515)
                     /locus_tag="OO145_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5466218.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO145_001019"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO145_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5466219.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO145_001020"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO145_001020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5466220.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO145_001021"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO145_001021"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5466221.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO145_001022"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO145_001022"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5466222.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO145_001023"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO145_001023"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5466223.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO145_001024"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO145_001024"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5466224.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO145_001025"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO145_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO145_001026"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO145_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5466225.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO145_001027"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO145_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5466226.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO145_001028"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO145_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5466227.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO145_001029"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO145_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5466228.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO145_001030"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO145_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5466229.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO145_001031"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO145_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5466230.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO145_001032"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO145_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5466231.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO145_001033"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO145_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5466232.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO145_001034"
     CDS             complement(34461..34604)
                     /locus_tag="OO145_001034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466233.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO145_001035"
     CDS             34605..34883
                     /locus_tag="OO145_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466234.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO145_001036"
     CDS             complement(34930..37026)
                     /locus_tag="OO145_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5466235.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO145_001037"
     CDS             complement(37026..37763)
                     /locus_tag="OO145_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5466236.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO145_001038"
     CDS             complement(37760..38398)
                     /locus_tag="OO145_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5466237.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO145_001039"
     CDS             complement(38512..38754)
                     /locus_tag="OO145_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466238.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO145_001040"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO145_001040"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5466239.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="OO145_001041"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="OO145_001041"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5466240.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10670 a        11025 c        10525 g        10650 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggttaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgatcat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta agtcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcgggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga ataggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggttg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc actatgaggt agcctgagtt taacggacac tccttcctga aatagaatgg
    42841 catcagaagg agctaataat gagcagaaaa
//
LOCUS       DAJTMX010000019        42201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTMX010000019 DAJTMX010000000
VERSION     DAJTMX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.019"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(2..298)
                     /locus_tag="OO145_001042"
     CDS             complement(2..298)
                     /locus_tag="OO145_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5466241.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="OO145_001043"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="OO145_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5466242.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO145_001044"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO145_001044"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5466243.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="OO145_001045"
     CDS             complement(1784..2323)
                     /locus_tag="OO145_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5466244.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="OO145_001046"
     CDS             complement(2353..3096)
                     /locus_tag="OO145_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5466245.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="OO145_001047"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="OO145_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="OO145_001048"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="OO145_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5466246.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="OO145_001049"
     CDS             complement(4151..4657)
                     /locus_tag="OO145_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5466247.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="OO145_001050"
     CDS             complement(4703..5203)
                     /locus_tag="OO145_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5466248.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="OO145_001051"
     CDS             complement(5289..5468)
                     /locus_tag="OO145_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5466249.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO145_001052"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO145_001052"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5466250.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO145_001053"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO145_001053"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5466251.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO145_001054"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO145_001054"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5466252.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO145_001055"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO145_001055"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5466253.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO145_001056"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO145_001056"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5466254.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO145_001057"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO145_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5466255.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="yciV"
                     /locus_tag="OO145_001058"
     CDS             12652..13533
                     /gene="yciV"
                     /locus_tag="OO145_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5466256.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="OO145_001059"
     CDS             13530..14150
                     /locus_tag="OO145_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5466257.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="OO145_001060"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="OO145_001060"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5466258.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO145_001061"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO145_001061"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5466259.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="OO145_001062"
     CDS             complement(15750..16508)
                     /locus_tag="OO145_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5466260.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="OO145_001063"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="OO145_001063"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5466261.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="OO145_001064"
     CDS             complement(17813..18064)
                     /locus_tag="OO145_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5466262.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="OO145_001065"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="OO145_001065"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5466263.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="OO145_001066"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="OO145_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5466264.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22520..22684
                     /locus_tag="OO145_001067"
     CDS             22520..22684
                     /locus_tag="OO145_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5466265.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22687..22854
                     /locus_tag="OO145_001068"
     CDS             22687..22854
                     /locus_tag="OO145_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466266.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22968..23063
                     /locus_tag="OO145_001069"
     CDS             22968..23063
                     /locus_tag="OO145_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466267.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23227..25902
                     /gene="acnA"
                     /locus_tag="OO145_001070"
     CDS             23227..25902
                     /gene="acnA"
                     /locus_tag="OO145_001070"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5466268.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO145_001071"
     CDS             complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO145_001071"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5466269.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26726..27490
                     /gene="pgpB"
                     /locus_tag="OO145_001072"
     CDS             26726..27490
                     /gene="pgpB"
                     /locus_tag="OO145_001072"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5466270.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27639..27947
                     /gene="lapA"
                     /locus_tag="OO145_001073"
     CDS             27639..27947
                     /gene="lapA"
                     /locus_tag="OO145_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5466271.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27954..29123
                     /gene="lapB"
                     /locus_tag="OO145_001074"
     CDS             27954..29123
                     /gene="lapB"
                     /locus_tag="OO145_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5466272.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29316..30053
                     /gene="pyrF"
                     /locus_tag="OO145_001075"
     CDS             29316..30053
                     /gene="pyrF"
                     /locus_tag="OO145_001075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5466273.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30053..30379
                     /gene="yciH"
                     /locus_tag="OO145_001076"
     CDS             30053..30379
                     /gene="yciH"
                     /locus_tag="OO145_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5466274.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO145_001077"
     CDS             complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO145_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5466275.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30992..31741)
                     /locus_tag="OO145_001078"
     CDS             complement(30992..31741)
                     /locus_tag="OO145_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5466276.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31831..32004)
                     /locus_tag="OO145_001079"
     CDS             complement(31831..32004)
                     /locus_tag="OO145_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466277.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO145_001080"
                     /pseudo
     CDS             complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO145_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            34180..34283
                     /locus_tag="OO145_001081"
                     /pseudo
     CDS             34180..34283
                     /locus_tag="OO145_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO145_001082"
     CDS             complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO145_001082"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5466278.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(36363..37490)
                     /locus_tag="OO145_001083"
     CDS             complement(36363..37490)
                     /locus_tag="OO145_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5466279.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO145_001084"
     CDS             complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO145_001084"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5466280.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(38790..39143)
                     /locus_tag="OO145_001085"
     CDS             complement(38790..39143)
                     /locus_tag="OO145_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5466281.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO145_001086"
     CDS             complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO145_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5466282.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO145_001087"
     CDS             complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO145_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5466283.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO145_001088"
     CDS             complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO145_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5466284.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(41888..>42201)
                     /gene="sapB"
                     /locus_tag="OO145_001089"
     CDS             complement(41888..>42201)
                     /gene="sapB"
                     /locus_tag="OO145_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5466285.1"
                     /translation="ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMV
                     FSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYAL
                     R"
BASE COUNT        10627 a        10414 c        10811 g        10349 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggcgtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatatattct cttttgttac ttatttctat accgtgcaca atatttttat
    22321 ccctcatatt tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat
    22381 tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg
    22441 taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt
    22501 acaccaatca ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc
    22561 ttctggaaat caggaacccc gccgtgatcc tgaattaaaa cgtaaagcct ggctggcggt
    22621 ttttcttggt tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg
    22681 ataactatgg tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat
    22741 gaaccagaaa cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg
    22801 cagcagcaag cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca
    22861 tcattcttat tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca
    22921 gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa
    22981 atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac
    23041 tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt
    23101 gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt
    23161 gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga
    23221 ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa
    23281 acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta
    23341 cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcagtt
    23401 accgaagagg atatccacgc actggcggga tggctgaaaa atgcccatgc tgaccgtgaa
    23461 attgcctacc gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt
    23521 gatctggcgg caatgcgcga agcggttaaa cgcctcggtg gcgatactgc aaaggttaac
    23581 ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat
    23641 gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg
    23701 ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc
    23761 atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagcga attgcaggac
    23821 ggtgaatgga ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc
    23881 aacggccttg gcgtgctggg gtggggcgtg ggtgggatcg aagcagaagc cgccatgtta
    23941 ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac cggaaaatta
    24001 cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat
    24061 ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact accgttggcg
    24121 gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca
    24181 atcgatgcag taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggtcgag
    24241 ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt
    24301 tttaccagta tgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa
    24361 cgcccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa
    24421 ctgaaagtga atgccacgca taaagatcgc ctgccagtcg attatgttat gaacggacat
    24481 cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc
    24541 tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactcttggc
    24601 ctcaagcggc aaccgtgggt caaagcgtcg ttggcaccgg gttcgaaagt cgtttctgat
    24661 tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa ccttgtggga
    24721 tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca
    24781 atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa ctttgaaggc
    24841 cgtatccatc cgctggttaa aactaactgg ctggcctcgc caccgctggt ggttgcctat
    24901 gcgctggcgg gaaatatgaa tatcaacctg gcttctgagc ctatcggcca tgatcgcaaa
    24961 ggcgagccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta
    25021 gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca
    25081 gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc
    25141 tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat
    25201 attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct
    25261 ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt
    25321 gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc
    25381 ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg
    25441 acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag
    25501 caggagcaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt
    25561 gactgggcgg caaaaggacc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt
    25621 gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa
    25681 ggcgtgacgc gtaaaacgtt agggctaacc ggggaagaga agattgatat tggcgatctg
    25741 caaaatctac aacctggcgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag
    25801 gaagtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac
    25861 gacggcattt tgcattatgt cattcgtaat atgttgaagt aacaagtatt tgcttgccgg
    25921 ttattttgct tccggcaagc aaatgaatta cacgatgcaa gagggttatt tgttcagcaa
    25981 atggcccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac
    26041 aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt
    26101 cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca
    26161 aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc
    26221 gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc
    26281 ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc
    26341 ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc
    26401 ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcaac
    26461 atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt
    26521 tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt
    26581 ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg
    26641 ttcacggaat ggcacaaatc tgtcaatttt tcttggaact ggcattttca gttatgattg
    26701 tgggacttat caaaaaggag aggccatgcg ttcgattgcc cgacgtaccg cagtgggagc
    26761 tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg
    26821 agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg
    26881 gggcgtcatt acacatttga ttttgttcgg ctggtttctc tggtgtctgc gttttcgcat
    26941 taaggctgcc tttgtattat ttgccattct ggcggcggca atccttgttg gacaaggcgt
    27001 taaatcctgg atcaaagaca aagtacagga accacgacct tttgttatct ggttggaaaa
    27061 aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct
    27121 agtaaaagaa cagttggctg aagagaaaaa tattccacaa tatttgcgtt cacactggca
    27181 gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact
    27241 gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgttggt
    27301 ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct
    27361 ggtggtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg
    27421 aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaattgcac aacgagaaca
    27481 agaaagttaa cgctggttga ttttccgaat ttaggcctta aatcatcaac aatgcgcgtg
    27541 gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac
    27601 cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct
    27661 tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg
    27721 tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat
    27781 tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc
    27841 gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg
    27901 cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg
    27961 agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa
    28021 gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg
    28081 ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta
    28141 aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg
    28201 gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct
    28261 atgaacagcg tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat
    28321 acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg
    28381 cgctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg
    28441 ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct
    28501 actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc
    28561 taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg
    28621 tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtcatatccc
    28681 aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg
    28741 gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg
    28801 atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gctgcacagg
    28861 tctatattac acgccagctt cagcgtcatc cgaccatgcg tgtattccat aagctaatgg
    28921 attaccactt aaatgaagcg gaagaagggc gcgccaaaga gagtctgatg gtgctgcgtg
    28981 acatggttgg cgagaaggtg cgtagtaagc ctcgttatag ctgccagaaa tgtggtttta
    29041 ccgcatacac cctctactgg cattgtccgt cttgtcgtgc ctggtcaact attaaaccga
    29101 ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag
    29161 ttattgatgt cccattgtgc tccggcaacg acggagcaga aaaagcctgc taggggaaaa
    29221 atcgcaactg ttaatttttt attttcacgg gtagaatgct cgccgtttac ctgtttcgcg
    29281 ccactcccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt
    29341 cccgcgccgt tacgaattct cctgtggttg ttgcccttga ttatcataat tgtgatgacg
    29401 cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggtaaagaga
    29461 tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct
    29521 ttcttgacct gaaattccac gatatcccca acactgcagc gcacgctgtc gctgctgcag
    29581 ctgacttagg cgtgtggatg gtgaatgttc atgcctccgg tggggcgcgt atgatgaccg
    29641 cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag
    29701 tgttgaccag catggaagcc agcgacctgg ccgatcttgg cgtgacactg tcacctgcag
    29761 attatgcaga acgtctggca gcactgacgc aaaaatgtga ccttgatggt gtggtgtgtt
    29821 ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacac
    29881 cgggcattcg tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac
    29941 aggcgttggc ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag
    30001 atccagcgca gacactgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga
    30061 ttccaacagc cgtctggtct actcaacgga aaccgggcgt attgatgagc ccaaagcggc
    30121 cccagtacgc cctaaaggcg atggagcggt gcgtattcag cgtcagacca gtggacgtaa
    30181 aggtaagggc gtttgcctga ttaccggtat cgatctcgat gatgccgaac tgacaaaact
    30241 tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga
    30301 aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt
    30361 aaaactcgca ggcggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt
    30421 actgtgtgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
    30481 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
    30541 agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc
    30601 ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact
    30661 cagagacatt gccaaagtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
    30721 cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc
    30781 taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg
    30841 caggtgataa ttacttcctg ttatatcgct gataataatt ttatatcttg agagtgttaa
    30901 taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat gatttgccaa
    30961 actgcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga acggtgagtt
    31021 tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta atcacgcgat
    31081 tgaatcgctc aacgggaccg atggagtatg aatgtacagc accaaatttc gagctgtcag
    31141 tcaggactat cgcttcgcac tctttttcca gcacggcatt gaccacatcg gtgcgcatca
    31201 tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc aataccaata aatgctttgc
    31261 tgaaatgtac ctgctggatg cactggcgtg tcaaagggcc aaccatactt tcgctttttt
    31321 tctggtacac gccaccgagc aaaatgactt cacaaggcgc gtctttcagc aaatgcgcga
    31381 tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcccccaga gtccgggcga
    31441 gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga accagtgacg
    31501 cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacgg gtttcgacgt
    31561 catcactatc aagcgaaact gcaaagccat gtgcacggcg gaggtaactc agtttttcga
    31621 gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt gccagatcgg
    31681 ttacgctaac ctgaccctgg tcaatgacca tctgtagaat agtttgttgt cgggaattca
    31741 tcgctgtaat tcttataacg ttataatact aaataaaaaa atactgacgc gccgggcatc
    31801 ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc cacggtgtct tcgggatgtc
    31861 tggcgtagat tcagcaacct ggcgcaagag ttctgcttta agtatctcga gattatggat
    31921 cgcagagtgg taatcgcctg ccacaaggat atctaacaca atatcaatac gttcggcaac
    31981 gcgctgggca tcaaattcag acatcaagga catccttata gcaaaagggg gaaaatgaat
    32041 aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca acggctaatt atcatccagg
    32101 aatacgatat ataatgacgg gatatagcgc taagtatata tattcatcta cttatgcgcg
    32161 cttcagatag cgtttatacc agcgttcgaa ggcgacggcg ggcatcggtt tggcaaacaa
    32221 aaatccttgc cgctcattga tcccgttctt ggttaaaaaa gcatcttcct ttgcactctc
    32281 tacaccttcg gcgatcacct gaagattcaa tgcctgggcc acagcgacga tcgcccggac
    32341 cagtgactgc gagacaggtt gtttgtgaat atctcgaaca aaaacctggt caagtttgat
    32401 ggcatcgatc ggaaagcgcg ccagttgcga aagtgaagag tagccggtac caaaatcatc
    32461 cagatgcact tgcgcaccta gttggctaaa ttgttgaata acagacagtg ccagttcatc
    32521 attctcaatc agacaactct ctgtcagttc aatatctata gggcagtatt caaaattgag
    32581 ttcctggaga acctgtttca gggcggtgaa aatggtttga tcggcgagct gacgtgcaga
    32641 aatatttacc gccactcgca ggtttatgcc tttatcccgc cactttgcca cctggcgtac
    32701 gacatcgaga atcacccaac ggcctaaagg cacaattagc cctgactctt cggcgtagga
    32761 aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac tgccaacgta ctagtgcttc
    32821 cagactgcgc acttcgacac gctaggtgat tttcgataac caactgatcg ttttccagtg
    32881 ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc atttctggag
    32941 taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct gtgtcggcgt
    33001 gacgaataat agtcgcgctg tccgaaccat gttcgggaga gagcgaaata cctactgaac
    33061 agctggtata aacttcaatt aaaccaatac gaaaggggag gcgtaagcgg gtcaaaattc
    33121 gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc agaaactcat
    33181 cgccacctgg acgcgccaac acctggtcat gttcgagaca gcttaaaata gccaatgaca
    33241 cgtcgcgtaa taactggtca ccaaacaaat gcccataggc gtcgttgacc tttttaaaat
    33301 tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta atagcgtgat
    33361 cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg gtatttgcca
    33421 gaatacgcag tcgctcctga gtgcggcgct cttcggtaat gtcggtaccg gaacagatta
    33481 aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac agaaacagcc
    33541 gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg cttcgaaaaa
    33601 atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac agtttaaaca
    33661 cgctttaccc aatgacgtcg tgttctttca accctgtgta atcttcacat aaccgattga
    33721 agcgttggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta
    33781 cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aaccgccggt gtgtcatgcc
    33841 atgcagacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc agatgcacag
    33901 aaagatcgct ttcatcgacg gtcagactca tcatcaggct ggaggtgatg accgtcattt
    33961 ccctgatgcg agcagcctgt tcggcagata actgcaatgt acgatcaggt tctgtggttt
    34021 tggtggtaga aaagcgcaaa gcatcgctgc tttccgtcaa ccgccagtat ggattgtgcg
    34081 atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg catatcctcc
    34141 ggttaacagc ggagtccgcg gttaaataaa aggaacaaca tgacttacat gaaattaacg
    34201 gcggctaaac gcattgtttt tttatatatt tatttgtaat ccagttttgg aaaaacgcca
    34261 gttttcaaac gaaagtcagt taaaaaatct gcctggatat aacgaaggta gagcggggaa
    34321 ataaacggcc catccatgag gaatgggccg tgaaaggaga ttacgcgacc gggcgcgcaa
    34381 taatgctgcg ggtttccatg cggacttcgg caatggtgac gtcaataacg tcagttactt
    34441 tgtaaaccgt ttcaccttta atttgtacag tgccgttttc ctggctgcaa accagttcat
    34501 cgcgcacagc gtgtaagaaa ggtgccggaa taaaggcgat agcgccgtta tcaaccaaac
    34561 gcacacgcat gccgccacgg ctgatatcga caatttccgc cgcgaaacgg gtgccggtcc
    34621 cggctttatc tttcaggaag cgtgcgtata accagtcgcc aacatcacgt tctgccatcc
    34681 ggttgagacg gcgacgttcg gccatttgga cagtgatttc atcctgcgga cgcgtcgcag
    34741 tttcgccttt gataaccgct ttcagcagac ggtggttgat catgtcgcca tatttacgga
    34801 tcggcgaagt ccaggtggcg tatgcttcca gaccgaggcc aaagtgagga ccgggttcag
    34861 agctaatttc agcaaatgac tggaagcgac gaatgcggct gtcgaggaaa ccagttggtt
    34921 gcgcatccag ttcacgacgc agtttgcaga aaccgtccag cgtgagcact tcttcggcat
    34981 cgacatgcag accgtgcgtt ttcagcaacg ctgccagcgc gtcggcattc gccggatcaa
    35041 agcccatatg cacgttatag atgccaaaac cgagcttatc gcgcagtacg cgggccgcac
    35101 aaatgttagc ggcaatcatc gcttcttcga cgatacggtt ggcaatgcga cgaggctcgg
    35161 cgacgatatc cagcacttca cctttttcac cgagaataaa gcggtaatcc gggcgatctt
    35221 taaacaccag tgcgtggtta tgacgccact cgccgcggcg ttggcaaatt tgcgctagca
    35281 aacggacttg ttcggcaatt gcttcacttt caggcttcca gtcaccggta ttttccagcc
    35341 agtcagaaac ctggtcatac accagcttcg ctttggattc gatggtggcg gcaaagaatt
    35401 cgatattatc ttcaatggtg ccatcagcgg agagcgtcat gcggcatgcc agtaccgggc
    35461 ggacttcatt ggcgcgcagt gagcagagat cgtcagaaag ctcgcgaggc agcataggga
    35521 tgttgaagcc aggcagatag ttggtgaatg cgcgaatttt cgcggctttg tccagcttgc
    35581 tgccttcagc aatccacgcg gttggatcgg caatcgccac aatcagctga agtttgtcat
    35641 ccggcagcgc cttagcgaaa agggcgtcat ccatatcttc agtgctggca ctgtcgatgg
    35701 tgacaaaatc cagcgaggtc agatcttcac gaaccagacc ttcatcgagc atttcggtag
    35761 cgacgccgtc tggtgcttct ttttccagat tatggcgcgc aagggtaacc caccacggta
    35821 caaagtggtc gtcaccaaaa gtgatgtatt gtgtcagttc agcatagaaa gaacgatcgc
    35881 ctttcagcgg atgacggcgc atttcggcaa ccgcccagtc gccttcttta aactcgtggt
    35941 tcaggccacg ggctgcgcgg caaggaatgg cgtcttttaa aagtggatga tcaggaacga
    36001 tggtcagacg gtcatttttg ccctgaactt tacccacgaa acgagtcagg aacggttcaa
    36061 ccagttcttc tggctctgcg gattcacgtt ctttttcact gtggatcacc gcgataattc
    36121 ggtcgccatg catgactttt ttcatctgcg gcggcggaat gaaataactt ttttgtgcgt
    36181 cgacttccag gaagccaaag cctttttctg tggcttttac caccccttca gcgcgtggcg
    36241 tctgggaatg cagttgctgt ttaagctgcg ctagcagcgg gttgtcctga aacataattg
    36301 tctattttgg tggccattag agcggctgac agttttacgc gaatctgtct gacgcggcaa
    36361 ggttaatatg tctcacccaa cgcgattttt aagcgattta tccagccaca cagcccgctc
    36421 cataccagca gattaataat ctgcgttgat gatttttcgt gttcgagtaa agggctgaac
    36481 tgggcggcgc taaagcgatc gggtgctcgg gtcagccatt gtatcgcctg aacggtagcg
    36541 cgctcaacag aatgacggtc agcccatcgg ttaatttcat tttcgccctt gcgcagaagg
    36601 gtgctgattt ttttatattc atttgatgaa tccatacagg tggaaaaaca agcaggactg
    36661 ccatttatac gcgcgcataa caacgccgca agctgattat ccggctgtgc attgacccgt
    36721 gtgctgttta acaacttttc gagaagggaa agtgaaatag ggtggagaga gagtgtttcg
    36781 gccagttggc aaagttctgc agttgaactt tttgtgtgct cttcaggtag tttttccacc
    36841 tcatagctgc ttcgccagcg tattgattca tcagcaaaca gtgacgcgtc ggcgtagttt
    36901 tgtatcgcca gcccgggtaa ccagcgtacc ggatgcccga gataagcctg aaatgtcgca
    36961 attgtccgcg cctgaaagcc aataaaacca atgatttggt taatgatcac acagtcccag
    37021 acagtaagac cgacgtcatt cagatgctgg cgcgatcggt tgtcgataat agaaggcgag
    37081 ctagccagtt ggcgggcata ttgcgtgatt tgtgccaacc gatgattact ttccctggag
    37141 gaatcaggtc cggggagcgg cgtaagtcgc gccgaatagt agttacataa ccgctggacg
    37201 ccgcaaacct gagcaaccgt cagggccgtc gacaagcgtt cataagcggt gaaggtttgt
    37261 gaacgggtca cggtaagctg gtcagggaac aactccactg caagaacgcg cgcagggacc
    37321 agccatgaaa ggaagggggc cagcgtctct tcatcaagga tcacgtcgag cagaaaggga
    37381 tcgctgacct ttgcggcttc cgggaccaga ggcagaacgt catactccgt agtactggat
    37441 tgcgtttcat gataccagtg gcttttgccg gtgatgtggc gttgttccat gggcgctcct
    37501 tggtcgtaaa ggaaatcgtt atcctgacgc aaggcgggaa ggggagaaag acggatcggc
    37561 gataacaaat atcagaaagg tataacagag ataacgggcg gcagaacgcc gcccatcttt
    37621 accaacagaa cgattatttc agttcgagtt cgttcattgc agcgatgctg aaaccgccgt
    37681 caacgtggac tacttcaccg gagataccgg cagagagatc ggagcacagg aatgccgcag
    37741 agttacccac atcttcaata gtaactgtac ggcgaatcgg ggtaacggct tcgcaatgag
    37801 ccagcatttt gcggaagtct ttgataccgg aggccgccag agtacggatc ggaccagcag
    37861 agatggcgtt aacacgcaca ccttccggac ccatcgcgtt cgccatatag cgcacgttcg
    37921 cttccagaga cgcttttgcc agacccataa cgttgtagtt cgggatagcg cgctcagcgc
    37981 caaggtagga aagggtcagc agggcagaac ccggattcag catggagcgg caagcttttg
    38041 ccattgcaac gaagctgtag gagctgatgt cgtgggcaat tttgaagcct tcacgggtaa
    38101 cggcgttaac atagtcacca tccagctgat cgccaggtgc aaaaccaata gagtgtacga
    38161 aaccgtcaaa tttcggccaa actttcccca gttcagcgaa catggtgtcg atgctggcat
    38221 cttctgcaac atcgcactgc agaacgatgt cagaacccaa ttgagcggca aattcttcta
    38281 cgcggccttt cagtttgtcg ttctggtagg tgaatgccag ttcagctcct tcgcggtgca
    38341 tcgcctgagc gataccgtag gcgatggaca gtttgctggc aacaccggtt accagaatgc
    38401 gcttaccgga aagaaaaccc atagctttaa tccttattgt tgatgcttgt tgtgcctgaa
    38461 aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga taaacggtta ttataatcaa
    38521 cctggctgtg agtagctata gttaccaggt ccgaccggag caggctgcgg cagggggggc
    38581 gcttttcccc ttaccctaac cctctcccca gaggggcgag gggaccatat tgtgcaaata
    38641 ttgttacccc agcaacaaac aggctcatac agcccctaac ccttttatgg cgatggctgg
    38701 gatggttcag accttgccga atattctcca gcactgtctc catgttttca ccacaacagc
    38761 tattcggctc ggtcggcccc ctcgcccttt cagggtgagg gtgaccggcg gttcagttcc
    38821 tgcagaataa tctccagcac cgcctcctca ttacaatcaa tctcgttatt ccagaaacgc
    38881 agcacggtcc agccctgcga ctcaagccag ctagtgcgcc tggtatcata ggcaactgcc
    38941 aaatcatgct gcccaccatc cagctcaacg actacgcgcg ccgagcagca agcaaaatcg
    39001 agaatgtagc tccccaccgg atgttgacgg cgaaatttga aatcaccaaa acgtcggctg
    39061 cgaagatatc gccagagctt tcgttcctgc aaagtgagat tgcgtcttaa atcacgcgca
    39121 tttgatttta ttttatccat cacctcattc tgacaagatt taatctcttg tcagaatgag
    39181 gtgaataatc tggaaggagg attcagaaaa ttagcgatct ttacgccacg catccgctgt
    39241 caatgcttcg ccaaagtgcc cggcaatcag ccgtttggtg agttcatgca gcggcgatgc
    39301 cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc tgatgcatca ccagcacctg
    39361 gtcgctaatg tgcttcatca ttccgatatg ctgggtaaca taaatatacg aaatgccctg
    39421 tttttcctgt aactccagca tcagattaat caactgcgaa cgcattgaca tatccagtga
    39481 agcgagggct tcatcggcaa taatgacttt tgggcgcaat atcagcgcgc gcgccagacc
    39541 cagacgctgt ttttgtccgg gtgccaacat atgcggatag taactgacgt gatccggtag
    39601 cagcccaacc atacgcatcg tttcaataat ctgtttgcga cgctgttccg gttccaggtc
    39661 ggtgttcagg cgcagtggaa aatccagaat ttgtgagata cgttgacggg gattcaacga
    39721 ggtcgaagga tcctgaaaaa tcatgcgaat acgctgacta cggaaggaat aatcgccaaa
    39781 atgcagcgga tgatcgtcaa tcaataactc gccgctggta ggttctatca ttcccgccag
    39841 catttttgcc agtgtggatt tacccgaacc attctcgcca ataatcgcca gtgtctggcc
    39901 ttcacgtagc gtaaagctca agggttttac cgcttctacg gtctgacgac gaaaccaacc
    39961 ggtccggtag cggaacgttt tacttaaatt acgcacttca agcagcgttt cgatcatctc
    40021 actctttctc catgttcagc gggaaatgac aggcatagag atgatttttc gcccccgtca
    40081 aacgtggcgt cacaatgcat tctcgttgtg catacgggca acgtggcccc agacgacaac
    40141 caatcggtaa ctgttccagc aatgggatag cgccgggtag agtattgagg cgacttttat
    40201 gcggcatcgc gctgccgaag tctggtatcg cgcggatcag cgcctgggta taaggatgat
    40261 gtggcatcgt caccaactcc ttactcggcg cggtttccac tgtttgaccg cagtaaagca
    40321 cgttaatttt atccgcccat tggctaagca tttgtaagtc atggctgata agcaaaatag
    40381 tggtattgct gttttggttg agacgcgtca gcaggcgaaa gatttgcgcc tgggttgttg
    40441 gctccattga gtttgtcggt tcgtcagcaa tcagcagacg cggttgattc gccagtgcaa
    40501 tggctatcat cactttctga cattcacctt cggtcaactc atagggaaaa ctgcgcatcg
    40561 catctttgtg atctttaatc cccacgcggt gcagcagttc aatggcacgg cgtttgcgcc
    40621 agccaaaacg ctgccaccaa cggcctttat aggtccaggc tgggatgttt tgcatcaact
    40681 ggcgacccac acgttctgaa gggtcaagac acgactgcgg ttcctggaaa atcatcgaca
    40741 cgttatggcc aaccagtttg cgccgttcgc gtgcggagag acgcagcaaa tcgatatcat
    40801 caaaacgcat acggtcagca gtaacacgcc agttatcttt attcacccca caaatcgctt
    40861 tcgcaatcaa actcttgccg gaaccggatt caccaacaag accgcggatt tcaccttcgg
    40921 ttaaggtcat acttacccgg tcgacggctt taacccactc atcaccggtt ttaaattcaa
    40981 tggtcaggtt acgaatatcg agtaatggca ttattccacc cccgcaataa tcgcacgacg
    41041 aacgccatcg ccaaggaggt taaccaacaa cacgctaatc ataattgccg cacctggcag
    41101 catgacagtc cacggggcga cataaatcag ttccagcgca tcaccgagca tcgctcccca
    41161 ttcaggcgag gggagttgtg cgccgagatc gagaaagccc agcgcggcga tatcgagaat
    41221 tgccatcgac agtgcgcggg tgatctcggt gaccagcccg gcggtgatgt ttggcatcac
    41281 agcaaaccag agaatattca gcgttgatgc gccatccaga cgggcggcga taacgtactc
    41341 tttttccagt tcgtcatgca ccatgctgta aatcgaacgt accatacgcg gcagcaatgc
    41401 cagccagaca gcaaacatag cgtgggacaa actcggtccg gcaaacgcta caacgataat
    41461 tgccaacagc agcgaaggga tcgccagtaa ggtatccaga atatggttaa gcaccgctga
    41521 gcggaggccg tgtgtcgccc cggcgaaggt gcccagtacc aggccacaga tcgtcgcggc
    41581 aagcgtcacc acaaatgcgc cacccacggt tggcgcagct ccgctcagta aacggcttaa
    41641 aacatcacgt ccgaggtcgt cagtccccag gaagaaagaa acttcgccat agcgcgacca
    41701 tgatggcggc aataattgat aaccgagaaa ttgctggtcg atgccgtaag gcgcaaacca
    41761 gccgccaaaa atacacagta cagccagtcc cgcgcagccg tacaggccga ccatcgcaga
    41821 ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg cccggcgggc gcttttcgct
    41881 gtatacgcta tcgtaaggca taccattcct tatgtttcag agggttagcc atggcaccca
    41941 aaatatcaga aatcacgtta acaataatga ccagtgagcc acacaccatc actccggcgg
    42001 aaatggctgc gtaatcctgc tggcggatgg cgttaattaa ccagcgccct aaacccggcc
    42061 agctaaaaac catttcggtg atcatcgcca gcgtcagcat ggtggaaaac tgtaagccca
    42121 ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg gcgacgcaaa atagtaaagc
    42181 gcgacaaacc gcgggtcgcc g
//
LOCUS       DAJTMX010000020        41870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTMX010000020 DAJTMX010000000
VERSION     DAJTMX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.020"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO145_001090"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO145_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5466286.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO145_001091"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO145_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5466287.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO145_001092"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO145_001092"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5466288.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO145_001093"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO145_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5466289.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO145_001094"
     CDS             5718..6659
                     /locus_tag="OO145_001094"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5466290.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCCVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO145_001095"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO145_001095"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5466291.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFVMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO145_001096"
     CDS             7679..8929
                     /locus_tag="OO145_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5466292.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO145_001097"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO145_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO145_001098"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO145_001098"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5466293.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO145_001099"
     CDS             10866..12116
                     /locus_tag="OO145_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5466294.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO145_001100"
     CDS             complement(12172..13260)
                     /locus_tag="OO145_001100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5466295.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO145_001101"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO145_001101"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5466296.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO145_001102"
     CDS             complement(14194..15243)
                     /locus_tag="OO145_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5466297.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO145_001103"
     CDS             15342..16223
                     /locus_tag="OO145_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5466298.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO145_001104"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO145_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5466299.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO145_001105"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO145_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO145_001106"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO145_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OO145_001107"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OO145_001107"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5466300.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OO145_001108"
     CDS             21991..23067
                     /locus_tag="OO145_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5466301.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OO145_001109"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OO145_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5466302.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OO145_001110"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OO145_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5466303.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OO145_001111"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OO145_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5466304.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OO145_001112"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OO145_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5466305.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO145_001113"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO145_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO145_001114"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO145_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5466306.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OO145_001115"
     CDS             complement(30809..31048)
                     /locus_tag="OO145_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5466307.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO145_001116"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO145_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5466308.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO145_001117"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO145_001117"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5466309.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="OO145_001118"
     CDS             complement(32934..33629)
                     /locus_tag="OO145_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5466310.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="OO145_001119"
     CDS             complement(33626..34024)
                     /locus_tag="OO145_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5466311.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="OO145_001120"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="OO145_001120"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5466312.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO145_001121"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO145_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5466313.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO145_001122"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO145_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37377..38138
                     /locus_tag="OO145_001123"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="OO145_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="OO145_001124"
     CDS             complement(38255..38833)
                     /locus_tag="OO145_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5466314.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="OO145_001125"
     CDS             39003..39590
                     /locus_tag="OO145_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5466315.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="OO145_001126"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="OO145_001126"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5466316.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..>41870)
                     /gene="dld"
                     /locus_tag="OO145_001127"
     CDS             complement(40734..>41870)
                     /gene="dld"
                     /locus_tag="OO145_001127"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5466317.1"
                     /translation="DLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEA
                     DTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRH
                     ILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEK
                     VKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDY
                     FKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEW
                     YEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPA
                     EHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKNWQEVE"
BASE COUNT        10261 a        10290 c        11063 g        10256 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgtgtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg tgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc
//
LOCUS       DAJTMX010000021        41399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTMX010000021 DAJTMX010000000
VERSION     DAJTMX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.021"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..675)
                     /locus_tag="OO145_001128"
     CDS             complement(<1..675)
                     /locus_tag="OO145_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCR5466318.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVC"
     gene            927..1661
                     /gene="exbB"
                     /locus_tag="OO145_001129"
     CDS             927..1661
                     /gene="exbB"
                     /locus_tag="OO145_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5466319.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            1668..2093
                     /gene="exbD"
                     /locus_tag="OO145_001130"
     CDS             1668..2093
                     /gene="exbD"
                     /locus_tag="OO145_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5466320.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(2365..3249)
                     /gene="yghA"
                     /locus_tag="OO145_001131"
     CDS             complement(2365..3249)
                     /gene="yghA"
                     /locus_tag="OO145_001131"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5466321.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            3440..3934
                     /locus_tag="OO145_001132"
     CDS             3440..3934
                     /locus_tag="OO145_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5466322.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(3974..5014)
                     /gene="gpr"
                     /locus_tag="OO145_001133"
     CDS             complement(3974..5014)
                     /gene="gpr"
                     /locus_tag="OO145_001133"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5466323.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            5171..6058
                     /locus_tag="OO145_001134"
     CDS             5171..6058
                     /locus_tag="OO145_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5466324.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            6177..6464
                     /locus_tag="OO145_001135"
     CDS             6177..6464
                     /locus_tag="OO145_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5466325.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            6653..7771
                     /gene="hybO"
                     /locus_tag="OO145_001136"
     CDS             6653..7771
                     /gene="hybO"
                     /locus_tag="OO145_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5466326.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            7774..8760
                     /gene="hybA"
                     /locus_tag="OO145_001137"
     CDS             7774..8760
                     /gene="hybA"
                     /locus_tag="OO145_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5466327.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            8750..9928
                     /gene="hybB"
                     /locus_tag="OO145_001138"
     CDS             8750..9928
                     /gene="hybB"
                     /locus_tag="OO145_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5466328.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            9925..11628
                     /gene="hybC"
                     /locus_tag="OO145_001139"
     CDS             9925..11628
                     /gene="hybC"
                     /locus_tag="OO145_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5466329.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            11628..12122
                     /locus_tag="OO145_001140"
     CDS             11628..12122
                     /locus_tag="OO145_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5466330.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            12115..12603
                     /gene="hybE"
                     /locus_tag="OO145_001141"
     CDS             12115..12603
                     /gene="hybE"
                     /locus_tag="OO145_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5466331.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            12596..12937
                     /gene="hypA"
                     /locus_tag="OO145_001142"
     CDS             12596..12937
                     /gene="hypA"
                     /locus_tag="OO145_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5466332.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            13071..13319
                     /gene="hybG"
                     /locus_tag="OO145_001143"
     CDS             13071..13319
                     /gene="hybG"
                     /locus_tag="OO145_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5466333.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(13442..14308)
                     /gene="yghU"
                     /locus_tag="OO145_001144"
     CDS             complement(13442..14308)
                     /gene="yghU"
                     /locus_tag="OO145_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5466334.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            14513..16372
                     /gene="gss"
                     /locus_tag="OO145_001145"
     CDS             14513..16372
                     /gene="gss"
                     /locus_tag="OO145_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5466335.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            16664..18163
                     /gene="pitB"
                     /locus_tag="OO145_001146"
     CDS             16664..18163
                     /gene="pitB"
                     /locus_tag="OO145_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5466336.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(18212..18904)
                     /locus_tag="OO145_001147"
     CDS             complement(18212..18904)
                     /locus_tag="OO145_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5466337.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            19078..19791
                     /locus_tag="OO145_001148"
     CDS             19078..19791
                     /locus_tag="OO145_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5466338.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            19823..20581
                     /locus_tag="OO145_001149"
     CDS             19823..20581
                     /locus_tag="OO145_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466339.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            20627..21976
                     /locus_tag="OO145_001150"
     CDS             20627..21976
                     /locus_tag="OO145_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5466340.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYGKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            21976..22800
                     /locus_tag="OO145_001151"
     CDS             21976..22800
                     /locus_tag="OO145_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466341.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            22812..23372
                     /locus_tag="OO145_001152"
     CDS             22812..23372
                     /locus_tag="OO145_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5466342.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            23403..24473
                     /gene="lptF"
                     /locus_tag="OO145_001153"
     CDS             23403..24473
                     /gene="lptF"
                     /locus_tag="OO145_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5466343.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            24470..25549
                     /gene="lptG"
                     /locus_tag="OO145_001154"
     CDS             24470..25549
                     /gene="lptG"
                     /locus_tag="OO145_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5466344.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(25585..26756)
                     /locus_tag="OO145_001155"
                     /pseudo
     CDS             complement(25585..26756)
                     /locus_tag="OO145_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(26756..27004)
                     /locus_tag="OO145_001156"
     CDS             complement(26756..27004)
                     /locus_tag="OO145_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5466345.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(27037..27951)
                     /locus_tag="OO145_001157"
     CDS             complement(27037..27951)
                     /locus_tag="OO145_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5466346.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLAHGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(27948..29678)
                     /locus_tag="OO145_001158"
     CDS             complement(27948..29678)
                     /locus_tag="OO145_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5466347.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            30040..31181
                     /locus_tag="OO145_001159"
                     /pseudo
     CDS             30040..31181
                     /locus_tag="OO145_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(31305..32075)
                     /gene="glcC"
                     /locus_tag="OO145_001160"
     CDS             complement(31305..32075)
                     /gene="glcC"
                     /locus_tag="OO145_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5466348.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            32326..33825
                     /gene="glcD"
                     /locus_tag="OO145_001161"
                     /pseudo
     CDS             32326..33825
                     /gene="glcD"
                     /locus_tag="OO145_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            33825..34877
                     /gene="glcE"
                     /locus_tag="OO145_001162"
     CDS             33825..34877
                     /gene="glcE"
                     /locus_tag="OO145_001162"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5466349.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            34888..36107
                     /gene="glcF"
                     /locus_tag="OO145_001163"
                     /pseudo
     CDS             34888..36107
                     /gene="glcF"
                     /locus_tag="OO145_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            36112..36516
                     /locus_tag="OO145_001164"
     CDS             36112..36516
                     /locus_tag="OO145_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5466350.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            36538..38709
                     /gene="glcB"
                     /locus_tag="OO145_001165"
     CDS             36538..38709
                     /gene="glcB"
                     /locus_tag="OO145_001165"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5466351.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            39064..40650
                     /locus_tag="OO145_001166"
     CDS             39064..40650
                     /locus_tag="OO145_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5466352.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         9830 a        10190 c        11087 g        10292 t
ORIGIN      
        1 gcacacggca gtgccaatca tcgcatctga atgcccaacc agatatttgg tggcggcctg
       61 aatagaaaca tcgatgccaa aatccagcgc cttaaacagc acaccggctg cccaggtgtt
      121 gtcgatcata atgatggcat ccggcaccac actgcgcacg gcggcaacaa tcgccggaac
      181 gtcgtggact tccatagtga tggaacccgg cgattccaga aacacgattt tagtgtttgg
      241 ctgcagatgc ttaacgatat cggcaccaat cagcggatca aaccacgatg tcgttacgcc
      301 cagtttgctg aggattttgc tacagaaatc ctgactcggt tcataggcgg tgttggtcat
      361 caacacatga tcgccctgtt cgacaaaagc aagaatcgaa ttagcaaccg ctgccgcccc
      421 gcagggaaat agcgcgcagc ctgcgccacc ttccagttca cacatcgctt gttgtaagga
      481 aaaatgggtt aacgttccgc gccgtccata gaacaactcg ccattggcgc gattgcgcgt
      541 cgcgtgcttt ttggcttcca tactctcaaa taccagcgaa gaagcgcgct gaatcacgct
      601 atttaccgcg ccaagagtgt attttttgct gcgtcctgca ttcaccagtt gagtatcaag
      661 ctttttgtcc gccatgtcgg gattcctgtt tttatacgtc tggatgtcta aactagcatg
      721 aatattcgcg ggcgcatcct gaagaattgg cataaagcag aaaattttcg caaatggcgt
      781 gctggtgact tttttactgc gtaaacgcaa ggaaaagaaa gcaaaggtac ggcagtatgc
      841 aaatagtaat gagaacgact atcaattcga cgtcgttttg atattattat gcgcagattt
      901 tgtgacttgc gtcctggaga tacacagtgg gtaataattt aatgcagacg gacctttccg
      961 tctggggtat gtatcagtac gccgatattg tcgttaagtg cgtgatgatt gggcttattt
     1021 tggcctctgt agtcacctgg gcaatcttct tcagtaagag cgtagagttc ttcaatcaga
     1081 agcgtcgcct taagcgcgag cagcaactgc tggctgaagc acgttcctta aaccaggcca
     1141 acgatatcgc cgctgatttt ggtagcaaaa gcttaagcct gcatttgctc aatgaagcgc
     1201 agaatgagct ggaactgtca gaaggcagcg acgataacga aggtattaaa gaacgtacca
     1261 gcttccgtct ggagcgtcgg gtcgccgcag tgggtcgtca aatgggccgc ggtaacggct
     1321 atctggcaac catcggcgcg atttctccgt tcgttggtct gtttggtacg gtctggggca
     1381 tcatgaacag ctttattggt atcgcgcaaa cgcagaccac taacctggca gtcgttgcgc
     1441 cgggtatcgc agaagctctg ttagcaacgg caatcggcct cgtggcagcg attcctgcgg
     1501 tcgttatcta taacgtattt gcacgccaga ttggcggctt taaagcgatg ctgggtgatg
     1561 ttgcagcgca ggtattgttg ctgcaaagcc gtgacctgga tctggaagcc agcgccgctg
     1621 cgcatccggt tcgtgtcgca caaaaattac gcgcaggata atgtccgatg gcaatgcatc
     1681 ttaacgaaaa cctcgacgat aacggcgaaa tgcatgatat caacgtgacg ccgtttatcg
     1741 acgtgatgtt ggttctgctg attatcttta tggtggcggc accgttagcg acggtagatg
     1801 tgaaggtgaa cttgcctgct tctaccagca cgccgcagcc gcgcccggaa aaaccggttt
     1861 atctgtcggt gaaggcagac aactcgatgt ttatcggtaa cgatccggtc accgatgaaa
     1921 caatgattac ggcgttgaat gcgttaaccg aaggcaagaa agacaccacc atcttcttcc
     1981 gagcggacaa aaccgtcgat tacgagacgt tgatgaaggt aatggatacg ctgcatcagg
     2041 cgggttacct gaagataggt ctggtcggcg aagaaaccgc caaagcgaag taaagtagaa
     2101 ttgcctgatg cgctacgctc atcaggccta caaaatctat tgcaacatgt tgaatcttcg
     2161 tgcgtttgta ggccggataa ggcgttcacg acgcatccgg cattaggtgc tcaatgcctg
     2221 atgcgctacg cttatcaggc ctacaaaatc tattgcaaca tgttgaatct tcatgcgttt
     2281 gtaggcggat aaggcgtttt cgccacatca ggtaagagtg aattcacaat gatgcccggt
     2341 tgcttttcac aaccgggcat ttttttaacc taaatgctcg ccgccgcaca cgccgtgcac
     2401 ttctgcggtg acgtagctcg actcctgact tgccagataa acatatacag gggccagttc
     2461 cgccggttgc cccgcacgct tcatcggcgt tttctgacca aactgcggga tcttatcctg
     2521 cgtttgtccg ccggaaattt gcagtgccgt ccagataggg cctggcgcga caatattcac
     2581 ccgaatacct ttctccgcga cctgttttgc caggccacgg ctgtagttca gaatcgccgc
     2641 cttcgtagcc gcatagtcca gtaaatgcgg acttggctgg tatgcctgga ttgacgaagt
     2701 ggtgatgaca cttgcacctt tcggtagcag ggggatcgct tcctgggtta gccagaacag
     2761 cgcgaaaacg ttaatggcaa aggtcttttg aaactgttcg ctggtgaggt ctgcaatatc
     2821 cggaatggca acctgtttcc cggcgaccag cgccataata tccagcccgc ctaacgcctt
     2881 gtgcgcttcg tgaaccagcg aacgggcgaa tttctcatcg cttaaatcgc ctggcagcag
     2941 aacggctttg cgtccgcatt cttcaatgat ctttttcaca tcctgagcgt cttcttcttc
     3001 cacgggaaga taactgatcg ccacgtcagc cccttcacgc gcgtaagcga tggcggcagc
     3061 gcgacctatt ccggaatcgc cccctgtcac cagtgcttta cgatctttca ggcgaccgct
     3121 accaacatag gttttctcgc cgcaatccgg tatcggtgtc atcttcgcct ggatgcctgg
     3181 cgtcggttgt ttctgtttgg gatattcacc agtgtaatac tgcgtggtcg ggtcttttaa
     3241 atgagacatc gtttttctcc cttcaggttc aacgtccttt aagggtagac gctctcgatg
     3301 cgttgataag ggaaccagga agatccctaa acctcagaat tatgcgacaa aggtttaacg
     3361 tatatgttga tttgctgttg cgtgctgttt actcaattgc gatatactgt tgcccgtttt
     3421 aactacacga caggaatgta tggaacgttt tcttgaaaat gcaatgtatg cttctcgctg
     3481 gctgcttgcc cccgtgtact ttggcctttc gctggcgtta gttgccctgg cgctgaagtt
     3541 cttccaggag attattcacg tactgccgaa tatcttctcg atggcggaat cagatttgat
     3601 cctcgtgttg ctgtcgctgg tggatatgac gctggttggc ggtttgctgg tgatggtgat
     3661 gttttccggt tatgagaatt tcgtctcaca gctggatatc tccgagaaca aagagaagct
     3721 gaagtggctg gggaaaatgg acgcaacgtc gctgaaaaac aaagtagcag cgtcgattgt
     3781 ggcaatttct tccattcact tactgcgcgt ctttatggat gcgaaaaatg tcccggataa
     3841 caaactgatg tggtacgtca ttatccatct gacgtttgtg ctttctgcat ttgtgatggg
     3901 ctatcttgac cgactgactc gtcataatca ctgatctgat tcgggcgcgg ttcgcgcgcc
     3961 cgttattaac aggtcattta tcggacgacg cctgccacag attcagctcg ccatcggcga
     4021 tatgctgatc aatctgcgcc agctcctcgg tgctaaatgt cagattattc agcgcctgca
     4081 cgttctcctc tagttgctcc gcgcggctgg caccaatcaa taccgacgtc acgcgatcat
     4141 ctttcagcaa ccagcttaac gccatttgcg ccattgattg tccacgctgc tgtgccattt
     4201 cattcaataa gcgcaggctg ttgaggttgg cttcggtaag cattttcggc gtcagaccac
     4261 gaactttatt cccttcacga tgcatccgtg aatcttgcgg aatgccgttg agatattttc
     4321 cggtcagcaa tccctgagcc agaggagtaa aggcaataca gcccacgccg ttattttgca
     4381 gggtatccag caggccgctt ttatccaccc agcggttcag taaattgtac gaaggttgat
     4441 gaattaaaag cggaattttc cactcgtgca gcaactcgac cattttttgc gtccgctctg
     4501 gcgagtaaga ggagatcccg acatacagcg ccttaccgct ttgtaccgca tgagccagcg
     4561 cagaggcggt ttcttccatc ggcgtatttt catcgacgcg atgagagtaa aagatatcga
     4621 catactcaag ccccatacgc ttcaggcttt ggtcgaggct ggcgagcagg tatttacgtg
     4681 aaccgccaga gccgtacggg ccgggccaca tatcgtagcc agccttggta gagataatca
     4741 gttcatcgcg ataagcagca aaatcctcgc gcagcaggcg accaaagttc tcttctgcgc
     4801 ttcctggagg cggcccgtaa ttgttggcta aatcaaagtg cgtaatgcct aaatcaaacg
     4861 ctttacgcag gattgcacgc tgtgattcca gcgcgttaac gtgaccgaaa ttgtgccata
     4921 aaccgagcga taatgcgggc aggcgtaaac cactttttcc gcaatagcgg tactgcatct
     4981 gcccgtaacg ttcgggattc gctaaccaga ccatgacctc tcctttccac cgttcaattt
     5041 cgaaacaatg tttctagttt agcgattcgc cagcgcgtat cccgtagtct ggctcacaga
     5101 gtgacgaaaa actgggcaaa aacacgcgct tatgctttgc ttaaaaaaac accagttgag
     5161 gaatgcaacg atgccgcgtt taaccgccaa agatttccca caagagttgt tggattacta
     5221 cgactattac gttcacggga aaatttcgaa acgtgagttc ctcaaccttg cggcgaagta
     5281 tgcggtgggc gggatgacgg cattagcgtt gtttgatttg ctcaagccaa attatgcgct
     5341 ggcgactcag gtagagttta ccgacccgga gattgttgct gagtacatca cgtatccttc
     5401 gccaaatggt cacggcgagg tacggggtta tctggtgaaa cccgcaaaaa tgagcggcaa
     5461 aacgccagcc gtgggggtgg tgcatgagaa tcgtggactg aatccgtata tcgaagatgt
     5521 ggcacggcga gtggcgaagg cggggtatat cgccctggca cctgacggat taagttccgt
     5581 tggaggttat ccgggaaatg atgataaagg tcgtgagctg caacagcagg ttgatccaac
     5641 caaactgatg aatgatttct ttgccgcaat tgagtttatg caacgctatc cgcaagcgac
     5701 aggtaaagtg ggtattaccg gattttgcta tggcggtggc gtatcgaacg cggcggctgt
     5761 cgcgtatccg gaactggcct gcgcggtgcc gttttatggt cgtcaggcac ccactgccga
     5821 tgtggcgaag attgaagcgc ctttactact ccacttcgcg gaactggaca cccgaatcaa
     5881 cgagggctgg cctccttacg aggcggcgtt gaaagacaat aataaggttt atgaggctta
     5941 tatctatccg ggggttaatc acggattcca taatgattcc acgccccgtt atgacaaatc
     6001 tgccgccgat cttgcctggc aaaggacact gaaatggttc gataaatatc tctcctgata
     6061 ggtttatctc ttacgggatt acgtcttaaa caagtatgaa aaaatagcgt gcgcaaaagt
     6121 cgttctttgc ctaaaatatc gttatatatg gcgatatata atgaatgagg tgaacgatga
     6181 ataaccattt tggtaaaggc ttaatggcgg gattaaaagc aacgcatgcc gacagtgcgg
     6241 ttaatgtgac aaaattctgt gccgattata aacgcggttt tgtattaggc tactcacaca
     6301 ggatgtacga aaagaccgga gatcgccagc ttagcgcctg ggaagcgggt attctgacgc
     6361 gccgctatgg actggataaa gagatggtaa tggatttctt tcgtgagaat aattcctgtt
     6421 ctacgttgcg cttttttatg gccggttatc gcctcgaaaa ttgatcaaac atacgtatta
     6481 tcttgcttta attaattaca ctaatgcttc ttcccttcgt tttagcgccc cgccgcagta
     6541 tcatgatatc gataaccata ataaatgtgt ggtaaatggc gcatcgatcg cattattgat
     6601 tttgcgattg aggcaaaata tatgccaggt tctcgcaacg gaataactat aaatgactgg
     6661 agataacacc ctcatccatt ctcacggcat taaccgtcgt gatttcatga agctttgtgc
     6721 agcattagcc gccaccatgg ggttaagtag caaagccgct gcagagatgg ccgaatcggt
     6781 tactaacccg cagcgtccgc cagttatctg gattggcgcg caggagtgca ccggttgtac
     6841 ggaatctctg cttcgtgcaa cgcatccaac ggtagaaaac ctcgtgctgg agactatctc
     6901 tctggaatat cacgaagtgc tttccgccgc cttcggtcat caggtcgaag agaacaaaca
     6961 taacgctctc gagaagtaca aagggcagta tgtgttagtg gtggatggtt ccatcccatt
     7021 aaaagataac ggtatttatt gcatggttgc cggtgagccg attgtggatc acatccgcaa
     7081 agcggcggaa ggctcagcag ccattatcgc tattggttcc tgctccgcgt ggggcggtgt
     7141 tgccgcagct ggagttaacc caactggcgc agtcagcttg caagaagttc tgccaggcaa
     7201 aaccgttatc aatattccgg gctgcccgcc gaacccgcac aacttcctcg cgaccgttgc
     7261 gcacatcatc acttacggca aaccgccgaa actggatgac aaaaaccgtc cgaccttcgc
     7321 ctatggccgt ctgattcacg aacactgcga acgtcgcccg cacttcgatg ctggtcgttt
     7381 tgccaaagag ttcggtgatg aaggccaccg cgaaggctgg tgcttgtacc acctcggctg
     7441 taaagggcca gaaacttacg gcaactgctc aacgctgcaa ttctgcgatg ttggcggtgt
     7501 gtggccggtg gcgattggtc acccgtgcta tggctgtaac gaaaaaggta tcggcttcca
     7561 taaaggcatc catcagcttg ccaacgtcga aaatcagact ccgcgttcac agaaaccgga
     7621 tgttaacgct aaagagggcg gcaacgtctc tgcaggcgct attggtttgc tcggcggtgt
     7681 ggttgggctg gttgccggtg tcagcgtgat ggcggtgcgt gaactgggtc gtcagcaaaa
     7741 gaaagataac gctgactcac ggggagaata accgtgaaca gacgtaattt tattaaagca
     7801 gcctcctgcg gggcattgct gacgggcgcg ctgccgtctg tcagtcatgc ggctgctgaa
     7861 aaccgcccgc caattccggg atcgctgggg atgttgtacg actcgacctt gtgcgtaggc
     7921 tgccaggctt gcgtcaccaa gtgtcaggat atcaacttcc ctgaacgtaa cccgcaaggg
     7981 gaacagacct ggtcgaacaa cgacaaactg tcgccgtata ccaataacat cattcaggtg
     8041 tggaccagcg gcacaggggt caacaaagac caggaggaga acggctacgc gtacattaag
     8101 aaacagtgta tgcactgcgt cgatccgaac tgtgtctctg tgtgcccggt ctctgcactg
     8161 aaaaaagatc cgaaaaccgg cattgtccat tacgacaaag acgtgtgcac cggttgccgt
     8221 tactgcatgg tcgcctgtcc gtacaacgtg ccgaagtacg actacaacaa cccgtttggt
     8281 gcgctgcata agtgcgagct gtgcaaccag aaaggtgtgg aacgtctcga taaaggcggt
     8341 ctgcctggct gcgtagaagt gtgcccggcg ggcgcggtga ttttcggtac gcgtgaagag
     8401 ctgatggcgg aggcgaaaaa acgtctggcg ctgaagcctg gcagcgaata ccactatccg
     8461 cgtcagacgc tgaaatctgg cgacacttac ctgcatacgg tgccgaaata ttatccgcat
     8521 ctgtacggcg agaaagaggg cggcggtact caggttctgg tactgacggg tgtgccttat
     8581 gaaaatctcg acctgccgaa actggacgat ctttctaccg gtgcgcgttc cgaaaatatt
     8641 caacacaccc tgtataaagg catgatgcta ccactggctg tgctggcggg cttaaccgtg
     8701 ctggttcgtc gcaacaccaa aaacgaccat cacgacggag gagacgatca tgagtcatga
     8761 tccacaaccg ctgggcggca aaatcatcag taaaccggtc atgatttttg gaccgttaat
     8821 cgtcatctgt atgctcctga ttgtgaagcg tctggtgttc ggtctgggct ctgtctctga
     8881 cctgaacggc ggcttcccgt ggggcgtgtg gatcgcgttt gacctgctga ttggcaccgg
     8941 ctttgcctgt ggcggctggg cgctggcgtg ggcggtatac gtctttaacc gtgggcaata
     9001 ccatccgctg gtgcgtccgg cgctgttggc gagtctgttt ggttactcac tgggtggctt
     9061 gtcgatcact atcgacgtgg gtcgctactg gaacctgccg tacttctaca ttccgggtca
     9121 cttcaacgtg aactcggtac tgttcgagac ggcggtctgt atgaccatct acatcggcgt
     9181 gatggctctg gagtttgctc cggcactgtt tgaacgtctg ggctggaagg tgtcgctaca
     9241 acgtctgaac aaggtgatgt tcttcatcat cgcgctcggt gcgctgctgc cgaccatgca
     9301 ccagtcttca atggggtcgc tgatgatctc ggcgggctac aaggtgcatc cgctctggca
     9361 gagctatgaa atgttgccgc tgttctcgct gctgacggcg ttcatcatgg gcttctcgat
     9421 tgtcatcttt gaaggttcac tggtgcaggc gggtctgcgt ggcaacggtc cggatgaaaa
     9481 gagcctgttc gtcaagctga ccaacaccat cagtgtgttg ctggcgattt tcatcgggct
     9541 gcgctttggc gagcttatct atcgcgacaa gctgtcgtta gcgtttgccg gtgacttcta
     9601 ctccgtgatg ttctggattg aagtcctgct gatgctcttc ccgctggtcg ttctgcgtgt
     9661 ggcgaagctg cgtaatgatt cccgtatgct gttcctgtca gcactgagcg cactgttagg
     9721 ttgtgcaacc tggcgtctga cctattcgtt ggtggcattc aacccgggcg gcggttacgc
     9781 ctacttcccg acctgggaag aactgttgat ttctattggt tttgtggcta ttgagatttg
     9841 cgcttacatc gtactcattc gtctactgcc gatacttcct cctttaaaac aaaacgatca
     9901 taatcgtcat gaggcgagca aagcatgagc cagagaatta ctattgatcc ggtaacccgt
     9961 attgaggggc atttacgcat cgattgcgaa atcgaaaatg gcgtcgtttc gaaagcatgg
    10021 gcttccggta ccatgtggcg cggcatggaa gagatcgtga aaaaccgcga tccgcgcgat
    10081 gcatggatga ttgtgcaacg tatctgtggc gtatgtacta ccactcacgc gctgtcttcc
    10141 gttcgtgcgg cagaaagtgc gctgaatatc gacgttccgg ttaacgcgca atacatccgt
    10201 aacatcattc tggctgcgca caccacgcat gaccatatcg ttcatttcta tcagctttcg
    10261 gcgctggact gggtggacat cacttctgca ctgcaagctg acccaaccaa agcctccgaa
    10321 atgctgaaag gcgtttcgac ctggcatctg aacagtccgg aagagttcac taaagttcag
    10381 aacaagatca aagatctggt tgccagcggt cagttgggta ttttcgccaa tggctactgg
    10441 ggtcacccgg cgatgaaact gccgccggaa gtgaacctga ttgcggtagc gcactacctg
    10501 caggcgctgg agtgccagcg tgacgctaac cgcgtcgtgg cgctgctggg cggtaaaacg
    10561 ccgcacattc agaacctggc tgtgggtggt gtcgcgaacc caatcaacct cgacgggttg
    10621 ggcgtgttga accttgagcg cctgatgtac atcaagtctt tcatcgacaa actgagcgac
    10681 tttgttgagc aggtttataa ggttgatacc gcggttatcg ccgcgttcta cccggaatgg
    10741 ctgacgcgcg gtaaaggtgc ggtgaactac ctgagcgtgc cggaattccc gaccgacagc
    10801 aaaaacggca gcttcctgtt cccgggaggc tacattgaga atgcggatct gtcctcgtat
    10861 cgtccgatca cttctcattc cgatgaatac ctgatcaaag gcattcagga aagcgcgaag
    10921 cactcctggt ataaagacga agcgccacag gcgccgtggg aaggtaccac cattccggct
    10981 tatgatggtt ggtctgacga cgggaaatat tcctgggtga aatcaccgac tttctacggc
    11041 aaaacggtag aagtgggtcc gctggctaat atgctggtga aactggcggc aggtcgcgaa
    11101 tctacccaga acaaactgaa tgaaatcgtt gcgatttatc agaaactgac tggcaacacg
    11161 ctggaagtgg cacagctgca ctccacgctg ggccgtatta ttggtcgtac cgttcactgc
    11221 tgtgaattgc aggatatcct gcaaaaccaa tacagtgcac tgatcaccaa tatcggcaaa
    11281 ggcgatcaca ccacctttgt gaagccgaac attccggcaa cgggtgagtt caaaggtgtt
    11341 ggcttcctcg aagcgccgcg cggtatgctc tctcactgga tggttattaa agacggtatc
    11401 atcagcaact accaggcggt tgttccatca acctggaact ctggtccgcg taacttcaat
    11461 gatgacgtcg gtccttacga gcagtcgctg gtgggtacac cggttgccga tccgaataaa
    11521 ccgctggaag tggtgcgtac cattcactcc ttcgacccgt gcatggcctg tgcggtacac
    11581 gtagtggatg ccgacggcaa cgaagtggtt tcagtgaagg ttctgtaatg cgtattttag
    11641 tcttaggggt cggcaatatt ttgctgaccg atgaagccat cggcgtgcgg attgtcgaag
    11701 cgttagagca acgatacatt ctgccggatt atgttgagat cctcgatggc ggcacggcgg
    11761 gaatggagct gcttggcgac atggcaaatc gcgatcatct gattattgcg gatgccattg
    11821 tgtcgaaaaa gaacactccg ggaacgatga tgatcctgcg ggatgaagaa gttccggcgt
    11881 tgtttaccaa caaaatctct ccgcatcagc ttggcctggc cgacgtattg tcggccctgc
    11941 gcttcaccgg tgagtttccg aaaaagctga ccctggtcgg cgtgatcccg gaatcgctgg
    12001 agccacacat cggcttaacg ccgacggttg aagcaatgat tgaacctgcg cttgagcagg
    12061 ttctggctgc gctgcgcgaa tcaggcgtgg aagccatccc acgggaggcg attcatgact
    12121 gaagagatag caggtttcca gacctccccg aaggcgcaag tacaggcagc gtttgaagaa
    12181 attgcccggc gttcgatgca cgatctctct tttctgcatc cttcaatgcc ggtgtatgtt
    12241 tctgatttta cgctgttcga aggtcagtgg acggggtgtg tgatcacccc gtggatgctg
    12301 agtgcagtta tcttccccgg cccggatcaa ctctggccgc tgcgcaaagt gagtgaaaaa
    12361 attggtctgc aactgccgta tggcaccatg acctttaccg ttggcgaact ggacggtgtt
    12421 tcgcaatacc tctcctgttc gctgatgtcg ccgctttcgc acagcatgtc gattgaagaa
    12481 ggccaacgcc tgacggatga ctgcgcacga atgatccttt cgttgccagt caccaatccg
    12541 gatgtaccac acgcagggcg tcgcgccctg ctgtttggcc gcaggagggg cgaaaatgca
    12601 tgagttgtcg ctttgccaga gcgccgttga aattatccaa cggcaggcgg agcagcacga
    12661 tgttaagcgc gtcaccgccg tgtggctgga aattggcgcg ctctcctgcg ttgaggagag
    12721 cgccgtccgt tttagttttg aaattgtctg ccacggaacg gtggcgcaag ggtgcgattt
    12781 acatatcgtc tataaacccg cccaggcctg gtgctgggat tgcagccagg tggtggagat
    12841 tcatcagcac gatgcgcagt gtccgctctg tcacggcgag cggttgcgtg tcgataccgg
    12901 cgattcgctg atcgtcaaaa gtattgaagt tgaataagac tgaggacaac gcctaaaaag
    12961 cgcacggacg gtcccctcgc ccctccgggg agagggttag ggtgagaggg aacacccgct
    13021 aaaggttctt tttcgcgttt gtccggcaat cgaaataacc ggagttaaca atgtgtattg
    13081 gcgttccagg ccaggtgctg gctgtcggtg aagatattca ccagcttgcg caggttgaag
    13141 tatgtggtat caagcgcgac gtgaatatcg ccctgatttg cgaaggtaac cctgccgatc
    13201 tgctgggcca gtgggtgctg gtacacgtcg gatttgccat gagcatcatc gacgaagatg
    13261 aagccaaagc cacattagac gcactgcgcc aaatggatta cgacattacc agcgcgtgat
    13321 gattagcttt tgtaggcctg ataagacgcg gcagcgtcgc atcaggcatt gtgcacgatt
    13381 gccggatgcg gcgtgaacgc cttatccggc ctggatggct tgctgcgacg aacaccaacc
    13441 cttacccctg acgcttatct tctgtatttg tctcaaaatc actggcgtca tggcgctcat
    13501 gcaactgctc gttcagcggc ccgttggtgc ggttaacaat acgcccacgt ttcactgccg
    13561 gacgttcgcc cacttctttc gcccagcgtt gcacatgttt ataacttccc gcatccagaa
    13621 actctgcggc atcatacacg ccgccgagca ccacgttgcc aaaccacggc caaatcgcca
    13681 tatccgcaat ggtgtactca tcgcccgcaa caaacttatg ctgcgccagt tgcttatcca
    13741 gcacgtcgag caggcgcttg gcttccatgg taaagcggtt gatggcgtac tcaattttca
    13801 ccggcgcgta atggtaaaag tgaccaaaac caccgccgag gaacggcgcg gcaccctgta
    13861 accagaacag ccagttcaac gtttcagtcc gttttgccag atcctgcggc aggaagtagc
    13921 caaatttctc cgccagataa agcaggatcg aaccagattc aaacacgcgg atcggcggat
    13981 tatgcgtatg atcgcgcagc gccgggatct tcgagtttgg gttcacttcg acaaagccgc
    14041 tggagaattg atcgccatcg ccaatacgaa tcagccaggc gtcgtactct gcaccagtaa
    14101 cgcccagcgc cagtagctcc tcaagcataa tcgttacttt ctgaccgttc ggcgttccca
    14161 gcgaataaag ttgcaatgga tgtttgccaa cgggcagcgt tttttcatgc gtcggaccag
    14221 aaaacgggcg attgatattg gcgaacgcgc cgccagcgga tttatcccac gtccagactt
    14281 tcgcgggctg ataagtattg tctgtcatag tgagttgcct tctgagtggt agtgttgaag
    14341 cagtgtagca ggtcacttgc tgtacagaat gtgaattttt taaccctttg cgccagccgc
    14401 tgcgatgagg tgtatgatta gtctgacctc tgtccggtta atgtcgctga gcaggtaatc
    14461 agagcacaac aattacgcca actgcttagg ttgtattgtt aaaggtaaag tgatgagcaa
    14521 aggaacgacc agccaggatg ccccgttcgg gacattattg ggctacgccc caggtggggt
    14581 agcaatctac tcttcagatt acagttctct cgatccgcag gaatacgaag atgacgccgt
    14641 attccgtagc tatatcgacg acgaatatat gggccacaag tggcaatgcg ttgaatttgc
    14701 tcgccgtttt ctctttctga attacggtgt ggtctttact gacgtgggta tggcgtggga
    14761 gattttctcg ctgcgcttcc tgcgagaagt ggttaatgac aacatcctgc cattgcaggc
    14821 atttcctaac ggctcgccgc gtgcgccggt cgcgggtgcg cttcttatct gggataaagg
    14881 cggtgaattt aaagacactg gccatgtcgc catcattacc caattgcatg gcaacaaagt
    14941 ccgtattgcg gaacagaacg tgattcattc cccgttgccg caagggcaac agtggacgcg
    15001 cgagctggag atggtggtcg aaaacggctg ctataccctt aaagacactt ttgatgacac
    15061 caccattctg ggctggatga tccagacgga agatactgaa tacagcttac cgcagccgga
    15121 aattgcaggc gagctgctga aaatcagtgg agcgcgtctg gaaaacaaag gccagtttga
    15181 cggtaaatgg ctggatgaaa aagatccgct acaaaacgcc tatgtgcagg ccaacggtca
    15241 ggtgatcaat caggatccgt atcattacta caccattacc gagagtgccg agcaggagct
    15301 aattaaagcc accaacgagc tgcatctgat gtatcttcac gcaaccgaca aggtgctgaa
    15361 agatgacaac ctgctggcgc tgttcgacat tccgaaaatc ctctggccac gtttgcgtct
    15421 ctcctggcag cgtcgccgtc accatatgat cactggtcgt atggatttct gcatggatga
    15481 gcgtggcctg aaggtttacg aatacaacgc cgactccgcc tcctgtcata ccgaagcggg
    15541 cttgatcctc gaacgttggg cggagcaggg ctataaaggc aacggcttca atccggcgga
    15601 agggctgatt aacgaactgg ctggtgcctg gaaacacagt cgtgcacgtc cgtttgtcca
    15661 tatcatgcag gacaaagata tcgaggaaaa ctatcacgcg cagtttatgg agcaggcgct
    15721 gcaccaggcg ggctttgaaa cgcgtatctt gcgcgggctg gatgaactgg gctgggatgc
    15781 tgccgggcaa ctgattgatg gggaagggcg actggttaac tgcgtgtgga aaacctgggc
    15841 gtgggaaacc gcgtttgatc agattcgtga agttagcgac cgtgagtttg ctgcggtgcc
    15901 aatccgtacc ggtcatccgc aaaacgaagt gcgtcttatc gacgtattgc tgcgcccgga
    15961 agtgctggtc tttgagccgc tgtggacggt gatccccggc aacaaagcga ttctgccgat
    16021 cctctggtcg ctgttcccgc accatcgtta cctgctggat accgatttca ctgttaatga
    16081 tgaattggtg aaaacaggtt acgcagtgaa accgatcgcc ggtcgctgtg gtagcaatat
    16141 cgacctggtc agccatcatg aagaggtgct ggacaaaacc agcggtaaat ttgccgagca
    16201 gaaaaacatc tatcagcaac tgtggtgttt gccgaaagtg gacggtaaat acattcaggt
    16261 atgtaccttc accgttggcg gcaactacgg tgggacgtgt ttgcgcggtg atgaatcact
    16321 ggtcatcaaa aaagagagtg atattgaacc gttaattgtg gtgaaaaagt aatacatgtt
    16381 gtagcaaccc cgtactgatt atgaagaata attagtacgg ggattaatca aaatattcaa
    16441 tatttatata ttccaatatt tattcatttc atatgtgaat atattaacca gtggaatacc
    16501 tgtgttttga ttatttctaa aggttttgaa tgaatgctta ttgtctgata cacaagaaat
    16561 aacactcttt ttatcgttaa aaaatgatat ttcactttgc ccatgccgtt aaagtcactg
    16621 ataatgcgtc cgttcgtaaa ttcaaaatgg cgtaatctaa tatatgctaa atttatttgt
    16681 tggccttgat atatatacgg ggcttttgtt attacttgct ctggcatttg tgttgttcta
    16741 cgaagcaatc aatggttttc atgacacggc gaatgcggtg gcaaccgtta tttatactcg
    16801 tgccatgcag ccacaacttg ctgtggtgat ggcggcattt tttaactttt ttggcgtgtt
    16861 attgggcgga cttagcgttg cctatgccat tgtccatatg ttaccaatcg atttgttgct
    16921 gaatatgggg tcaactcacg gtttggcgat ggtcttttcc atgctgctgg cggcgattat
    16981 ctggaacctg ggaacgtggt tctttggttt accggcctcc agttcgcaca ccttgattgg
    17041 tgcgattatc ggcatcggtt taaccaacgc gctgttaacc ggctcatcgg tgatggatgc
    17101 gttaaacctg cgtgaagtaa ccaaaatttt ctcctcgctg attgtttccc ctatcgtcgg
    17161 cctggtcatt gcgggtggtc tgattttcct gctgcgccgt ttctggagtg ggacgaaaaa
    17221 gcgtgaccgt attcaccgca ttccggaaga tcgcaaaaag aaaaagggca aacgtaaacc
    17281 gccattctgg acgcgtattg cgctgattgt ttccgctgcg ggcgtggcgt tttcgcacgg
    17341 cgcgaacgac gggcagaaag ggatcggcct ggtaatgctg gtactggtgg ggattgcccc
    17401 tgctggcttc gtcgtcaata tgaatgcgtc cggctatgaa attacccgta cccgcgatgc
    17461 cgttaccaac ttcgaacact acctgcaaca gcatcctgaa ctgccgcaga agttgattgc
    17521 gatggaacct ccattgcctg cagcatcgac tgatggcacg caagtaacag agtttcactg
    17581 tcatccggca aatacctttg atgcgattgc gcgcgttaaa acgatgctgc caggcaatat
    17641 ggaaagttac gagccgttaa gcgtgagtca gcgcagccag ctgcgccgca ttatgctgtg
    17701 catctccgat acctccgcga agctggcgaa actgccaggc gtcagtaaag aagaccagaa
    17761 cctgctgaaa aaactgcgca gcgatatgtt aagcaccatt gagtacgctc cggtgtggat
    17821 catcatggcg gtagcactgg cgctcggcat tggcaccatg attggctggc gtcgtgtagc
    17881 gatgaccatc ggtgagaaga ttggtaagcg cggcatgacg tatgcgcaag gcatggcggc
    17941 acaaatgacg gcggcagtgt ctatcggcct tgccagttat attgggatgc ctgtctccac
    18001 aacacacgtc ctctcgtctg cggtagcagg gacgatggtg gtggacggcg gtgggttaca
    18061 gcgtaaaacg gtaaccagca tcctgatggc gtgggtgttt actttaccgg cggcaatttt
    18121 tctttctggt gggctgtact ggatagcatt gcagttgatt taaccttcct gaaaatgccc
    18181 ggtcctggcg atcgggcatt tccatttttg actagtgata accacgcgcg gtcataaaat
    18241 ccgtaatcgc tttttctgca tcaaccaaca cctgttccag tggctgattg gcatcgatat
    18301 caaccagttg tgcaccacca aaggttaact gtggcgttat ggcaatcttc ctcgccagcg
    18361 attcccgttt atggtcgggt ttacgtgcac aggcaacttc aaggtcaaca ttgagtttga
    18421 tgaccagatc aggcttatgg ctcgccatcc agtgaaacgc tttacgttcc tggcttgcca
    18481 gccatgagac aaaacgacca ccttcaacgt taggtgggaa caccgtacca tcgtaagcgc
    18541 caggaatttg gtcctgagga taacggtcgg ttagaacaat taacccgcga cgacgacagg
    18601 caagcatatg acgaaagcgc agtaagcgac gggcgacaaa cgctgtaatt accagcgccg
    18661 gaactggtcc aggcaatttt tttgctgttt tcacctgatt tcgttcaatt gttttatgta
    18721 aggattttcc catcaaaggt aattttgtca ctgcacgacc gacatttccg gcctgttttc
    18781 ctaaatgaac tctttcggca gcaccatatt tttcgacaac ggtaataaga tgttcacaca
    18841 ccgttgactt gcctgaagca tcgctaccaa taacggcaat taatggaggt gttattgact
    18901 gcatgaatat atcctttaat ttaaatatcc attaaaaata tttatttggt taatatgttt
    18961 ttatgaaagc gtaattcagg tcaatgtcac aattagtcat tgtcacaata aggttgaacg
    19021 gatattctga gcacggacca cgtaatattc aatacattat ttactgccgg gtttttcgtg
    19081 aatcaacgta atatgtcaat aattaattcc acgccagtgc gtgttattgc cattgtagga
    19141 tgtgatggtt caggtaaatc gaccctcacg gcaagcctgg taaatgaact ggcagcaaga
    19201 atgccaacag aacacattta tctcgggcaa tcgtccgggc gaattggcga atggatttca
    19261 cagctccctg ttattggcgc accttgtggg tgttatctgc gaagtaaagc ggcacatgta
    19321 cacgaaaagc ccttaactcc gcctggcaat attactgcgc tggttatcta tctgctttcc
    19381 tgctggcggg cgtacaagtt tcgcaaaatg ttgtgtaaaa gccagcaagg ctatctgctc
    19441 atcaccgacc gctacccgca ggttgaagtg ccgggttttc gctttgatgg cccgcaattg
    19501 gcaaaaacca cgggcggtaa cggttggata aaaatgttaa ggcagcgcga gttgaagctg
    19561 taccaatgga tggcgtctta tttacccgta ttgttgattc gtcttggcat tgatgaacaa
    19621 accgcgtttg cgcgtaaacc tgaccaccaa ctggcagcgt tacaggaaaa aatcgccgtt
    19681 acgccgcaac tgacattcaa tggcgccaag atccttgaac tggatggtcg gcaaccagcc
    19741 gatgaaatta tgcaagcgtc actacgcgca attcacgccg cgctttcctg atcaccaaaa
    19801 tggatagggg atgatttaac gaatggatgc actacaaaca caaactgtta atagtacaac
    19861 cgcgccgcag cccaactaca ttccggggct gattgcggtg gtcgggtgtg atggcaccgg
    19921 taaatccacg ctgaccaccg acctggtgaa atcgctgcaa caacactggc aaaccgagcg
    19981 ccgctatctg gggctgctct ccggcgaaga cggcgacaaa atcaaacgat tgccgttggt
    20041 tggcgtctgg ctggaacggc gattggcggc caaatcctcg aaaacccaaa gcatgaaaac
    20101 caaatctccg gcgctatggg cggcggtgat tatgtactgc ttctcgctgc gaagaatggc
    20161 gaatctacgc aaggttcagc gactggcgca aagtggcgtt ctggtggtca gcgatcgctt
    20221 cccgcaggct gaaatttcgg gcttttatta tgacggaccg gggatcggcg tcgaacgtgc
    20281 gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg agtttatacc aaaaaatggc
    20341 gcaataccgt cctgaattaa tcattcgttt ggggattgat attgatactg ccatctcccg
    20401 caagcctgac catgagtatg ccgagctgca ggacaaaatc ggcgtcatgt cgacgattgg
    20461 ctataacggc acaaaaattc ttgagattga ttccagagcg ccctacagcg aagtgctgga
    20521 acaggtacag aaggcggttt ctctggttgc catcgtttct gaccgccgaa gtttaactta
    20581 aggctggcgg gatatttttt actatcaacg ataaggtcag gctgaattgg cgggttttaa
    20641 catcaaacat tggtttgcag atggcgcgtt tcgcaccatt attcgcaata gcgcctggtt
    20701 aggctccagt aatgtcgaga gcgccttgct ggggctgttg gcgctctcgt gtgccggtaa
    20761 agggatgacg cccgccatgt ttggtgtact ggtgattgtg caatcgtacg gcaagtcgat
    20821 cagcgatttt attaagtttc agacatggca gctggtggtt cagtacggaa cgccagcctt
    20881 gaccaacaat aatccgcagc aatttcgcaa tgtcgtctca ttttccttct cgctggatat
    20941 cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta ctgccattcc tttcccattc
    21001 attaggtctg gatgaccaaa gcttttggct ggcggcgctg tattgcacgc tcattccttc
    21061 aatggcttcc tccacgccga ccggcattct gcgtgcggta gatcgctttg atttgattgc
    21121 tgtacagcag gcgacgaaac cttttctgcg cgcagcgggg agcgtcgcag cctggtattt
    21181 tgactttggt tttgtgggtt ttgttattgc ctggtacgtg tcgaatctgg ttggcggcac
    21241 catgtactgg tggtttgccg cgcgcgaatt acgccgtaga aatatccata acgccttcaa
    21301 attgaatctg tttgagtctg cccgacacat taaaggcgcg tggagttttg tctggtcaac
    21361 caacattgcc cactccatct ggtcggcgcg taactcgtgc agcacagtgt tggtggggat
    21421 cgtgttagga cccgctgccg ccgggttatt taaaatcgcc atgacattct tcgacgccgc
    21481 cggaacgcca gcgggtttgc tgggtaaaag tttttaccca gaggtgatgc gtttagatcc
    21541 gcgcaccacc agaccgtggt tgttaggtgt gaagtccggt ttactggcgg gcggaatcgg
    21601 tattctggtg gcgctcgcgg tgttgatcgt cggcaagccg ctcatttcgc tggtgtttgg
    21661 cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa ttgtgatttc
    21721 gatgctgggc ttcccgcagg agtcattgct attgatggcg ggaaaacagc gcgcttttct
    21781 cgtggcgcaa accatcgcct caatcggcta catcgtattg ctgttcatgt tctgtcatct
    21841 gtttggtgtg ctgggagcag cgtttgctta cttcggtggg caatgtctgg acgtggcgct
    21901 ctcgttaatt ccaaccttaa aggcattttt tcagcgccat tccttgcttt ataacgcagc
    21961 cggagagaaa tcctgatgat gagaaaatat ttcccgcttg aggcgagcga gcgtctattt
    22021 gttgcgattg aagaagatga tgtcgttgat gcacaggtta gtttgccgcc gactattgca
    22081 ctaagttgta ccaccgaaat cattcatgat aattacgcgt tatgcctgca attctggctc
    22141 aatggcgttg atcgtcagga attactgcgt ttagttcgca aacaagccaa aggcgatgag
    22201 ctgactgctg atgagcgcaa acaatttaaa tacatgcgag cgcgctacaa gcacctgcgt
    22261 tttgcgcaac ggctgtattt aaagaagcat caggccggat ttttattcgg caaaacgacc
    22321 gtttttctgg ggcgttttca ggacggcttt cgtaacggta agaaaaatat tgtgtcgtat
    22381 tacggcaacc tgttacggat ttatttaagc tctccggtct ggtcgttggt gaactattca
    22441 ttacgccata gccagttaga aagcgtaagc agttttattg cctatcggca aaagcaaatg
    22501 cataccctga aagagattat tgccaaaccg cgactaaccg gcagggaatt tcatgatgtg
    22561 cgaaaaatca tcagccagca ggtttcttat tacgacacct taagatcgct tgatcctgaa
    22621 aataaagagg cgttacagat ttcccgtttc ctcgccgcca ttaatggcct gatgggagat
    22681 aaacacgacg acatggtggc ggatgatatg gaaaaccgcc agtcgtatta tgcaccgttg
    22741 gcgctggata gcgatatttg tcagcgctta gagctgctga tttcgcgttt tccgctatag
    22801 ccggagcaat gatgtcatct cgtttgatta ttgcgttgat tatcatgctc ttagccccgg
    22861 gtgtgcaggc acacaatttc gtcactggca aaacggttac ccctgtttat atccaggaag
    22921 gcggcgagct gcttctgaat agcgacgacg aaattcacta ccaaaagtgg aacagcacac
    22981 aattagcggg aaaagtccgc attattcagt atatcgccgg gcgtaagtcg gcgaagaaaa
    23041 agaactcact gttaattaaa gcggttgaag cggcaaactt tccgcaggat cgttttcagc
    23101 ccaccaccat tgtgaacacc gatgatgcca tctttggtac tggctatttt gttgttggca
    23161 aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt cgttattgac ggcaatggac
    23221 agggacgcgt tgcctggcgc ttgccggagc agagttcaac cattctggtg ctgaataaag
    23281 ccgggcaaat tcagtgggca aaggacggca gcctgacgcc ggaagaggta gatcatgtca
    23341 ttgcgttggc gcagaaactg atcaatgaat aaaaaactca aaggattgag ggcgttagtg
    23401 agatgaagct ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg ctgattatcg
    23461 tcggttttct gatctttatt ttcgccagct actccgcaca gcgttatctg accgaagcgg
    23521 caaatgggac attagctctt gatgttgtgc tggatatcgt tttctacaaa gtactgattg
    23581 cactggagat gttgttacct gttggtctgt atgtgtcagt tggcgtgacg ctagggcaga
    23641 tgtatactga ctcggaaatt accgctatct ctgcggcggg gggcagtccg ggacgcttgt
    23701 acaaagccgt tctttatctg gcgataccgc taagtatttt tgtcaccctt ctgtcgatgt
    23761 atggtcgtcc gtgggcttat gcgcagattt atcaactgga gcagcagtcg cagtcggagc
    23821 tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa cgataacgga cgaatgatcc
    23881 tttcgcagac ggttgatcag gataataatc gcctgactga cgcgctgatt tatacttcta
    23941 ctgccaatcg aacccgtatt ttccgcgccg gttcggttga tgtggttgac ccatcacccg
    24001 agaaaccgac cgttatgttg cacaacggca ctgcctatct tctcgatcat caggggcgtg
    24061 acgacaacga acagatctac cgtaatctgc aattacatct gaatccgctg gatcaaagcc
    24121 cgaacgtcaa acgcaaagcg aaatcggtca cggagctggc gcgctccgcc tttcctgccg
    24181 atcatgccga actgcaatgg cgacaaagcc gtggcctgac cgcattgttg atggcgctgc
    24241 tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg attttcaacg ttattgccac
    24301 tgacgttgct gtttattgcc attttttatg gcggcgacgt ctgccgtaca ctggtggcta
    24361 acggcgcgat tcccctcatt cctggtttgt ggttagtgcc cggactcatg ctaatgggcc
    24421 tgctgatact ggtcgcacgt gacttctctt tgctgcagaa attttcccga tgaatgtttt
    24481 cagtcgctat ttaatccgtc atctctttct cggttttgcc gccgctgcag ggctattgct
    24541 gccgcttttt accaccttca acctgattaa cgaactggat ggtgtcagcc cgggcggtta
    24601 tcgctggact caggcggtgc tggtggtgct aatgacctta ccgcgcacac tggtcgaact
    24661 ttcgccgttt atcgcgttat tgggtggtat tgttggactg gggcagttat cgaaaaacag
    24721 tgagcttacc gccattcgca gcacggggtt ttctatcttc cgtattgcac tggtggcgct
    24781 ggttgccggg atattgtgga ctgtttcgtt aggcgcgata gatgagtggg tggcgtcgcc
    24841 attgcagcaa caggcgttgc aaatcaaatc gaccgccacc gcgttggggg aggacgatga
    24901 cattaccggc aatatgctgt gggccaggcg tggtaatgaa tttgtgacgg tgaaatcgct
    24961 gaacgagcag ggccagccgg tgggcgtgga gatatttcat tatcgcgacg atctctcgct
    25021 tgaatcctac atttatgcac gcagtgccac cattgaagac gacaaaacgt gggtcctgca
    25081 tggtgtgaat cataaaaaat ggctgaatgg caaagaaacg ctggaaacat tagataatct
    25141 tgcctggcaa tcggccttca ccagtatgaa tcttgaagag ttatcaatgc cggggaatac
    25201 tttttctgtc cgtcagctta atcattacat ccattatttg caggaaaccg ggcaacccag
    25261 cagcgaatac caccttgcac tgtgggaaaa actggggcaa cccatcctga ccctggcgat
    25321 gattttgctg gctgtgccgt ttacctttag tgccccgcgt tcgcctggga tgggtagccg
    25381 tctcgctgta ggcgtcatcg tgggcttact cacctggatc agctatcaaa tcatggttaa
    25441 tctgggattg ctatttgcgt taagcgcacc tgttaccgcg ctcggtttac cggtagcgtt
    25501 tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga caacattaaa cccgccgtgg
    25561 cgcaaacacc acggcgggct cacattactg cttcagagta gcaaatgcct gaatgatggc
    25621 atcgatttgc tcacgactgt gggcagcatt tacgctacag cgcagcagcg tgatgcctgc
    25681 aggcgcggca ggcggcagga taagattcac atacacgccc ggcgagataa gctcgcgcca
    25741 gatgcgtaaa ccttcctctt tcgaaccgat tattactggc actaccgggc taatatgtga
    25801 acttaactca taacccagtt tttgcagacc gtcatacaga tggttggcgt tatccatcag
    25861 cttttgccgc agctctggtt gtgtggcaat agttcttaac gacgagcgca cggtcgcaat
    25921 gcaggacgga gaaggagagg cggtaaaaat atacgggcgg ctgccgtagc gcagcacttc
    25981 catcgcttcc gaaacgacag caaaaccgcc aatcgaggcc agacttttac tgaaagtacc
    26041 aacgataata tccacatcat cttcaacgcc gacagcttca gccagtccgc gccccgttgc
    26101 gcctaacacg ccaaacgaat gcgcttcatc gacaatcaaa taaccaccga gacgacgttt
    26161 gatatcaaca atttccgcca gcggcgcaac gtcaccgagc atgctgtaga tgccctcaac
    26221 gatgataatc gcctctttcg cccgttcacc gaggcgaacc atgcgccgtt ccagatcttt
    26281 agcatcgtta tggcggaaac gaataatttc cgcgccaccg agagaacaga cgtcataaat
    26341 actggcgtgg ctgtcggcat ccagtaacac cacggcgtta tggtcggcaa gtgcgctgat
    26401 gacgcctaaa ttagcggtat aaccggttga aaaacgatag cggtagggcg attgaaaaac
    26461 tcggcaatct ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc catacgagaa
    26521 ccggtggtgc cggtgccttg ggccgctaac gcggcctgac cttcgctgat ggcatcatga
    26581 ttaaaggtta aaccgagata attattggtc ccggccagaa tcactttttt attgccgatc
    26641 cggccttcgg tggcggaata cacttcatca atacaggtgc caaacggtgt taacccgttg
    26701 tcactaaatt gcagacgctc gccagcaagg cgcgcatatt tatcgtatag ccccattatt
    26761 tattctccag ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca
    26821 gaatattaat aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca
    26881 ctttgatcga ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac
    26941 cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta
    27001 ccatagaaac gttaacacac ctttaaaaca agacaatcaa aatagcccgt tgcgtaatgc
    27061 gtgttccagg ctaatcccgg gaaaccaatt gatatcttca gaaatacggt tattacttgc
    27121 cgaccagtcg gcgtgggtta attcacgaat tttcgagcgg gtcagcatag gttctttacc
    27181 tgccaggcga ctcaacgcgg tactgatatc cgcaaggcct gtgagtaccg gcagaggaat
    27241 gtcaaccatt cgcacggaac cacaacgggc tttggcggca agctgtcgta cgcgttgcca
    27301 gtcatagcca ccggcgacgc catcgcataa ttcataggtt tgcgtctgta cagtttcggc
    27361 gcttaaccac tgacccactg cttgcgcaaa atcggtgacg tgaagaaaag agagttgtgt
    27421 atctggcgca ccaagtcgtg gcagcaggcc gcgtagcatc cagtcaaaca gcggttttaa
    27481 ctctttatcg ccgggaccat acacggctgt cgggcgaaaa acgcccagcg taatttcgtc
    27541 cgccattgct gccagtcgtt gctcggcgac gtatttggat tttgcatacc aggagagctc
    27601 gggatggcgc gccgccagcg aagagataaa cagaaaacgt tggcaaaagc cgctctcttt
    27661 tgctgcttgc atcaggcgca ggctgccgtc aacgttacag cgggtgaaaa tctcttcttt
    27721 gtgcccgcgc acttgcccgg cgcaatggac cactacgctg gctccggcaa ccagctcgct
    27781 aagtgaatga gtatcttcca gcgaaccgcg cacccaggtg agattatcat tgacgtgagc
    27841 gcgggcagta cgcgtcaatg cgcgaacgtg aaagccgtgg gcgagcaggt tatcgataat
    27901 atatttaccg ataaacccgg tagcgcccgt caccgcgaca gtttgattta tgccagggat
    27961 tcctgcacat taagactggc agcataagcc ttctgataac gttttttcgc ttccgcacgg
    28021 gcaggcttgc cggaggacgt tcggggaata ctgtggggcg gcaacagatc gatagccgcg
    28081 gtcacgccaa attcgctttg gatccgtgcc gccagcgcgt ggataagctg cccgcgacgt
    28141 tcttcgtcgc tgatccgaca ctggatctgc aaaatgattt tttcctgggc ggtaacaaaa
    28201 gcaatcgcat cgccagaatg aatttccggt tcctgttccg ctatatattc aatatcctgc
    28261 ggccagatat tacggccacg aataataatc agatctttaa tgcgtccggt gacatacaga
    28321 taaccgtcca gcagataacc gaggtcgccg gtgtctaacc agcccgtcgc ggcaatctcg
    28381 tcttgcgaaa cctggtcgcc aaagtaaccg ctcatcagac tgggaccgga gatgcaaata
    28441 tggcctacca cacgttccgc gaccggcata cctgcttcat tgcggatttc aataccatgt
    28501 tccggcaacg ctttgccgca gttgacgaaa gtcgatacgg cgcgtgtctc tgcacccggc
    28561 gcgacggctt taccctgata ttcgaggatg tcgcgatcca cttcgttaac cacaaccccg
    28621 gaggcttcat cagagaagct gacagccagc gcattttctg ccagtccgta gcacggcatg
    28681 aaagttttat tgtcaaagtt aacctgacgg aaacattcag cgaattgatg gagttgttct
    28741 gcggagatcg gctcagcacc aataccagcg acgcgccagc aggaaagatc cagttcagcg
    28801 agatcttttt cattcacgcg gcgttggcac aattcatagc caaacggcgg cgcaacggaa
    28861 acggtgccgc gatttttact gatcaattta agccattgca aaggacgcat ggcaaaatcc
    28921 tgagtgcgca aataatctac tgaaagttgc gtggcgacgg ggggcaggag aaagccgacc
    28981 agtcccatat catggtagaa aggctgccag gagacgcagc ggtcgctagg gcgtaattta
    29041 atgccatcgt ggcttatagc acgtagatta gccatcactt cgcgatgggt gataatgacg
    29101 ccacggggaa aacgggtgct gccggaggtg tactggaggt aggcgatatc gttcggaact
    29161 ggacgctgga gcgcaacatc ggcttccggt aatgccttaa accaagcgtg gcttaaaaca
    29221 tgtaattcgg ggttgttatg cgtcgcggca ttgaccagtg gcaaccactc atcaccagtg
    29281 ataatggctg cgggctggca acttgccagt aaaccctgga gtttggcgct ccaggaatcc
    29341 cgctgaccga cgcccattgg aatcgccaac gggacggcga ctaagccagc atactgacag
    29401 gcaaaaaaag cctctacaaa cccgctactc gtttcggcaa tcagcgccac gcgttcgcct
    29461 tttttcaggt tcagcgataa caaccgcttc gcaccagctt cggcacgcgt ttttaatgtc
    29521 tgatattcca gttgatcttc aagttggcaa cgtctgtcat aaaaattcat tccggctcta
    29581 ctcagagcgg cgtagtccaa agcatcaacc agcgttggaa aatcggcata gcgcataggt
    29641 agggaatgcg taaagatttt attagacata tacaccacga aaaaattgga tatcaaaaat
    29701 aaaatgacaa taatgcggtg gattatatag gcataaaaga taaatgagat agtattttca
    29761 tcggtgtgaa aatgaacatt gatttaaata atgagtttaa gttttttgtt tttattatga
    29821 atgatattta atttatgaat atattaagaa gaagccttgt ccggtcgcct taaattgaca
    29881 aaaggcattg aggcgtttct gtgtagcctc tgaccttatc ataatcatta attaatacta
    29941 tttattcgtc gtttgtattc attgagttga ttattaagat gccgctcgtt gctgttgcaa
    30001 cctgtttttc accattaatt atttgttatc cacagagata tggaatgcga tctgctgatg
    30061 attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc ttttccgtca
    30121 tcactttatc ccgacgatcc aaactggata ccccctttat tcattgaacg caacgagcat
    30181 ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt ggcaaaaaaa
    30241 gcggggcaga tagttgggcg tattaccgcg caaatagata ccttgcaccg tgagcgttac
    30301 ggtgaagata ccggtcattt cggcatgatt gacgccattg atgatccgca ggattttgcc
    30361 gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa gatcagcggg
    30421 cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt tgacacacca
    30481 ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga acaactgggt
    30541 tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct cactttctct
    30601 ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaagg tgaccattcg ctgcatcaat
    30661 cgtcagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaactc tggctggcag
    30721 cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg cgaccaactt
    30781 aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc accctgcgcg
    30841 tttattgtgg cttgcctaac atcaacgagg cgattgccga tctgaacgga tcgctctttc
    30901 ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga actgcgcgag
    30961 taccgctgat gggcgtgcgc gacgagtatc agttcagccg catcggcccg gtgattgcgc
    31021 tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat gcgctggaga
    31081 tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc attggggcgg
    31141 agccgtacaa gcgttaccga ttgtatgaaa agcaaattta ataccagatc ctgtttagcg
    31201 caggggcggt cattcgggag agggtataga tgaacagatt taacaccaga tcctgttaag
    31261 tgcaggggcg gtcccctcgc cttggggata gggtaaaact ctgctcacat tcccaccttg
    31321 ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc cttccagctc gatatcgtgg
    31381 agattctttt tcacggtccg tacatgatcg cgcgctgcgc gctgagcgac gtgcggcagc
    31441 cgctgcaaca ccgcgttgta gatccgcgca tgctggcgat cgatctgctt tttttgctgt
    31501 ggtcgatggt agagattatt tacgctggca aacactgaat taaacatcag atcggtcaat
    31561 gattgcagcg taaacaccag cacctgattg tgagaagcct gacaaatggc gagatggaaa
    31621 gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt tgtggttctc actggcggcg
    31681 agcatttttt cataacagcg ggttatcaca acaaaatcag cctgcgttcc cagcgttgcc
    31741 gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt cgagcagatc gtacagcgtt
    31801 cgcggctgcg tactgaacag atggatcagc gggctggtgt cctgctcccg attaagccgt
    31861 gcgacatgag aatcgcgacc ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc
    31921 agcccttcac gcagtgcgga gcgtgagaag ccgagctttt cacacagtcg acgttccgag
    31981 ggaagcggct gaccgacctt cagtacgccg tcgataatta accgttcgat actctctgca
    32041 accacttcgc aaatagggcg acgttcatct ttcattcccg gacctcgtgc acaggtagga
    32101 ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac gcaagtgatt atgttacatc
    32161 aatttaacat taagttaacc aagacaaggt cacagagctg gaaaaaaaat ggtctgaccg
    32221 gtagctaaag agatagacga aaacgaaaag cccgcttaat aactgttcac agaagcagcg
    32281 cgcaaaaatc agctgccaca caacacaaca aagcgaagcc tactcatgag catcttgtac
    32341 gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca catcggtact gatggcactg
    32401 cgtgagcatg tccctggact tgagatcctg cataccgatg aggagatcat tccttacgag
    32461 tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg ttgttctgcc taagcaaatg
    32521 gaacaggtga cagcgattct ggctgtctgc catcgcctgc gtgtaccggt ggtgacccgt
    32581 ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg aaaaaggtgt gttgttggtg
    32641 atggcgcgct ttaaagagag cctcgacatt aaccccgttg gtcgccgcgc gcgcgtgcag
    32701 ccgggcgtat gtaacctggc gatctcctag gccgttgcgc cgcataatct ctattacgcg
    32761 ccggatcctt cctcacaaat cgcctgttca attggcggca atgtggcgga aaatgccggt
    32821 ggcgtccact gcctgaaata tggtctgacc gtacataacc tgctgaaaat tgaagtgcaa
    32881 acgctggacg gcgaggcgct gacgctggga tcggacgcgc tggattcacc tggttttgac
    32941 ctgctggcgc tgttcaccgg atcggaaggt atgctcggcg tgaccaccga agtgacggta
    33001 aaactgctgc cgaagccgcc cgtggcgcgg gttctgttag ccagctttga ctcggtagaa
    33061 aaagccggac ttgcggttgg tgacatcatc gccaatggca ttatccccgg cgggctggag
    33121 atgatggata acctgtcgat ccgcgcggcg gaagatttta ttcatgccgg ttatcccgtc
    33181 gacgccgaag cgattttgtt atgcgaactg gacggcgtgg agtctgacgt acaggaagac
    33241 tgcgagcggg ttaactacat cttgttgaaa gcgggcgcga ctgacgtccg tttggcacag
    33301 gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa atgcgttccc ggcggtagga
    33361 cgtatctccc cggattacta ctgcatggat ggcaccatcc cgcgtcgcgc cctgcctggc
    33421 gtactggaag gcattgcccg tttatcgcag caatatgatt tacgtgttgc caacgtcttt
    33481 catgccggag atggcaacat gcacccgtta atccttttcg atgccaacga acctggtgaa
    33541 tttgcccgcg cggaagagct gggcgggaag atcctcgaac tctgcgttga agttggcggc
    33601 agcatcagtg gggaacatgg catcgggcga gaaaaaatca atcaaatgtg cgcccagttc
    33661 aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg cgtttgaccc cgatggtttg
    33721 ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg ctgaatttgg tgccatgcat
    33781 gtgcatcacg gtcatttacc ttttcctgaa ctggagcgct tttgatgcta cgcgagtgtg
    33841 attacagtca ggcgctgctg gagcaggtga atcaggcaat tagcgataaa acgccgctgg
    33901 tgattcaggg cagcaatagc aaagcctttt taggtcgccc agtcaccggg caaacgctgg
    33961 atgtgcgttg tcatcgcggc attgttaatt acgacccgac cgagctggtg ataaccgctc
    34021 gtgccggaac gccgctggtg gcgattgaag cggcgctgga gagcgccggg caaatgcttc
    34081 cctgcgagcc gccgcattat ggtgaagaag ccacctgggg cgggatggtc gcctgcgggc
    34141 tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga ttttgtcctc ggcacgcgca
    34201 tcattaccgg cactggaaaa catctgcgtt ttggtggcga agtgatgaaa aacgttgccg
    34261 gatacgatct ctcacggtta atggccggaa gctacggttg tcttggcgtg ctcactgaaa
    34321 tctcaatgaa agtgttaccg cgaccgcgtg cctctctgag cctgcgtcgg gaaatcagcc
    34381 tgcaagaagc catgagtgaa atcgccgagt ggcaactcca gccattaccc attagtggct
    34441 tatgttactt cgacaatgcg ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag
    34501 cagcgcgtga actgctgggt ggcgaagagg ttgtgggtca gttctggcag caattgcgtg
    34561 aacaacaact gccgttcttc tcgttaccag gtaccttatg gcgcatttca ttacccagcg
    34621 atgcgccgat gatggattta cccggcgagc aactgatcga ctggggcggg gcgttacgct
    34681 ggctgaaatc gacagccgat gacaatcaaa tccatcgcat cgcccgcaac gctggcggtc
    34741 atgcgacccg ctttagtgcc ggagatggtg gctttgcccc gctatcggct cctttattcc
    34801 gctatcacca gcagcttaaa cagcagctcg acccttgcgg cgtgtttaac cccggtcgca
    34861 tgtacgcgga actttgagga gcaggctatg caaacccgat taaccgaaga gatgcggcag
    34921 aacgcgcgct ggaagccgac agcatcctgt gcgcctgtgt tcactgcgga ttttgtaccg
    34981 caacctgccc aacctatcag cttctgggcg atgaactgga cgggccgcgc gggcgcatct
    35041 atctgattaa acaggtgctg gaaggcaacg aagtcacgct taaaacacag gagcatctcg
    35101 atcgctgcct cacttgccgc aattgtgaaa ccacctgtcc ttctggtgtg cgctatcaca
    35161 atttgctgga tatcgggcgt gatattgtcg agcagaaagt gaaacgccca ctgccggagc
    35221 gaatgctgcg cgaaggattg cgccaggtag tgccgcgtcc ggcggtcttc cgtgcgctga
    35281 cgcaggtagg gctggtgctg cgaccgtttt taccggaaca ggtcagagca aaactgcctg
    35341 ctgaaacggt gaaagctaaa ccgcgtccgc cgctgcgcca taagcgtcgg gttttaatgc
    35401 tggaaggctg cgcccagcct acgctttcgc ccaacaccaa cgcggcaact gcgcgagtgc
    35461 tggatcgtct ggggatcagc gtcatgtcag ctaacgaagc aggctgttgt ggcgcggtgg
    35521 actatcatct taatgcgcag gagaaagggt tggcacgggc gcgcaataat attgatgcct
    35581 ggtggcccgc gattgaagca ggtgccgagg caattttgca aaccgccagc ggttgcggcg
    35641 cgttcgtcaa agagtatggg cagatgctga aaaacgatgc gttatatgcc gataaagcgc
    35701 gtcaggtcag tgaactggcg gtcgatttag tcgaacttct gcgcgaggaa ccgctggaaa
    35761 aactggcaat tcgcggcgat aaaaaactgg ccttccactg cccgtgtacc ctacaacatg
    35821 cgcaaaagct gaacggcgaa gtggaaaaag tgttacttcg tcttggattt accttaacgg
    35881 acgttcccga cagccatctg tgctgcggtt cagcgggaac atatgcgtta acgcatcccg
    35941 atctggcacg ccagctgcgg gataacaaaa tgaatgcgct ggaaagcggc aaaccggaaa
    36001 tgatcgtcac cgccaacatt ggttgccaga cgcatctggc gagcgccggt cgtacctctg
    36061 tgcgtcactg gattgaaatt gtagaacaag cccttgaaaa ggaataaaaa aatgaaaact
    36121 aaagtcattc ttagccagca aatggcgagt gcaattattg ccgcaggtca ggaagaggcg
    36181 cagaaaaata actggtctgt ttccattgct gttgccgatg acggcggtca tctgctggcg
    36241 ttaagtcgca tggacgattg cgcgccgatt gcggcttaca tctcccagga gaaagcgcgt
    36301 accgccgcgc tggggcgtcg tgaaactaag ggctatgaag agatggtgaa caacggacgt
    36361 accgcgttcg tgactgcgcc gttactgacg tcgctggaag gcggcgtacc ggttgttgtt
    36421 gatgggcaaa ttattggtgc cgtgggcgtt tctggtttaa ccggagcaca ggatgcgcag
    36481 gtcgcgaaag tggcagcagc ggtgttggcg aaataagcga aaacgaggag agaaacaatg
    36541 agtcaaacca taacccaggg ccgtttacgc attgacgcca attttaaacg ttttgtggat
    36601 ggagaagttt tgccgggcgt ggaactggac gctgctgcat tctggcacaa tgttgatgag
    36661 atcgttcacg atctggcacc agaaaatcgt cagttgctgg cagaacgcga tcgcattcag
    36721 gcggcgcttg atgagtggca tcgcagcaat ccggggccgg taaaagataa agcggcctat
    36781 aaatctttcc ttcgtgaact gggctacctg gtgccgcaac cggagcgcgt gacggtggaa
    36841 accacgggca ttgacagcga aatcaccagt caggcggggc cacagctggt ggttccggca
    36901 atgaacgccc gctacgcgct gaacgcggcg aacgctcgct ggggctcact gtacgatgcg
    36961 ttatacggca gcgacatcat cccgcaggaa ggggcgatgg tcagtggcta cgatccgcag
    37021 cgcggtgagc aggttatcgc ctgggttcga cgtttcctcg atgaatcact accgctggaa
    37081 aacggcagct atcaggatgt ggtggcgttt aaggtggtcg ataaacaatt acgcatccag
    37141 ttgaaaaatg gtaaagaaac cacgttacgt actccagcac agtttgtcgg ttaccgtggc
    37201 gatgccgctg cgccgacctg cattttgctg aaaaataacg gcctgcatat tgaactgcaa
    37261 attgatgcca acgggcggat tggcaaagac gatccggcgc acatcaacga tgttatcgtc
    37321 gaagctgcta tcagtaccat tctcgactgc gaagattcgg tcgcggcggt tgatgcggaa
    37381 gataaaatcc tgctgtaccg caatctgctg ggcctgatgc aggggactct gcaagagaaa
    37441 atggagaaaa acggtcggca aatcgtacgt aaactgaatg acgatcgtca gtacaccgcc
    37501 gccgatggct ctgaaatttc tctgcacgga cgctcgttgc tgtttatccg caacgtgggt
    37561 catttgatga ccattcctgt gatttgggac agcgaaggca atgaaatccc ggaaggtatt
    37621 cttgatggcg tcatgactgg cgcgattgcc ctctatgatt taaaagtgca gaaaaactcg
    37681 cgcactggca gcgtctatat tgtgaaaccg aaaatgcacg gtccgcagga agtggcgttc
    37741 gccaacaaac tgtttacccg cattgagaca atgctcggta tggcaccgaa taccctgaaa
    37801 atgggcatta tggatgaaga acgccggacc tcgctgaact tgcgtagctg tatcgctcag
    37861 gcgcgcaacc gcgtggcgtt catcaatacc ggtttcctcg accgtaccgg cgatgaaatg
    37921 cattcggtga tggaagctgg cccgatgctg cgtaaaaacc agatgaaatc gacgccgtgg
    37981 atcaaagcct acgaacgtaa taacgtgctt tccggtctgt tctgtgggct gcgcggtaaa
    38041 gcgcaaatcg gtaaaggcat gtgggcaatg ccggacctga tggcagacat gtacagccag
    38101 aagggcgacc aactgcgtgc cggggcaaac acagcctggg ttccgtcacc aaccgctgct
    38161 acgctccatg cgctgcacta ccaccatacc aacgtacaga gcgtgcaagc caacattgcc
    38221 cagaccgagt tcagtgctga atttgaaccg ctgctggacg atctgctgac tattccggtg
    38281 gctgaaaacg ctaactggtc ggcgcaagag atccaacaag agctggataa caacgtgcag
    38341 gggattctgg ggtacgtggt gcgttgggtg gagcagggga ttggttgttc aaaagtgccg
    38401 gatattcaca acgtggcgct gatggaagac cgcgcaacgc tgcgtatctc cagccagcat
    38461 atcgccaact ggttacgtca cggtattctg accaaagaac aggtgcaggc gtcgctggag
    38521 aatatggcga aagtggttga tcagcaaaat gctggcgatc cggcttatcg tccgatggcg
    38581 gggaatttcg ctaactcgtg tgcttttaaa gctgccagcg atttaatctt cctcggcgtg
    38641 aaacagccaa acggttatac cgaaccgtta ttacacgcct ggcgtttacg cgaaaaagaa
    38701 agtcattaat agcgtcaaaa aagccccggc aagttttatt gtcggggcta tgatagtaac
    38761 tgcggtagta catttatccc ggaaataacc ggagcgatta tcagggttta acaatattaa
    38821 atacccgtag cgcattacta gcgggcctaa aacaacaata tggacatctt aacgatactc
    38881 tgcgcctgcg tgtgcggaac agaaaccctt acccgatata cttgttatgc cagaggtagc
    38941 aattgcgtta gcggcgtact gttgctgtaa agtataacgc ctatatttta cagttcagtc
    39001 ctcttttgct tattacaacc ttaagtatat caagcatata aagataataa gagactgaac
    39061 aatatggtta cctggaccca aatgtatatg ccgatgggag gactggggct atccgctctg
    39121 gtcgccctga tcccgataat attcttcttc gttgcactcg cggtattacg tctgaaagga
    39181 catgttgctg gagcaataac ccttatatta tctatcctga ttgcgatatt cgcctttaaa
    39241 atgccgattg atatggcatt tgctgctgcg ggctatggct ttatttatgg attatggcca
    39301 attgcgtgga ttattgtcgc ggcggtgttc ctgtataaat taaccgttgc cagcgggcag
    39361 ttcgatatta tccgcagctc ggttatctcc atcaccgacg atcagcgttt gcaggtgtta
    39421 ctgattggtt tctcctttgg tgcgttgctg gaaggagctg ctggctttgg tgcgacggtg
    39481 gcgtttggtg cgttgggcgt gccgattctg gtcgccggtc aggtaacggg aatcgatccg
    39541 ttccacattg gcgcaatggc gggacgtcag ttaccgttcc tgtcggttct tgtgccgttc
    39601 tggctggtag caatgatgga cggctggaaa ggggtgaaag agacgtggcc agcggcgctg
    39661 gttgctgggg gaagcttcgc tgtcactcag ttctttacct ctaactatat tggtccggaa
    39721 ctgccggata ttacttcggc gctggtgagt atcgtctcac tcgctttatt ccttaaagtc
    39781 tggcggccga aaaataccga aacggcaatc agcatggggc aatccgcagg tgcgatggtg
    39841 gtaaataagc catcttctgg cggtcccgtg ccttcagaat atagtctggg gcaaatcatt
    39901 cgtgcgtggt caccgttttt aatcttaacg gtgctggtca ccatctggac catgaagccg
    39961 tttaaagcgt tatttgctcc gggcggcgcg ttttattcac tggtgattaa tttccagatc
    40021 cctcatttgc atcaacaagt gttgaaagcg gcacccattg tcgcccaacc aacgccaatg
    40081 gatgcggtgt ttaaattcga ccctctctcg gctggcggca ccgctatttt tattgcggcg
    40141 attatctcta tcttcatcct cggcgtgggc atcaagaaag ggattggcgt ctttgccgaa
    40201 atgctaatta gtctgaagtg gccgatattg tcgattggca tggtgctggc attcgccttc
    40261 gtcaccaact attccggcat gtccaccacg ctggcgctgg tactggcagg tacaggcgtg
    40321 atgttcccgt ttttctcacc gtttctcggc tggctgggcg tattccttac cgggtcggac
    40381 acttcttcaa acgccctgtt tggttcactg caatcgacca cggcgcagca aatcaacgtc
    40441 tctgacactc tgctggtggc agcaaatacc agcggcggcg taactggcaa gatgatctcc
    40501 ccgcaatcta tcgccgtggc ctgcgccgcg acgggcatgg tgggccgaga atctgaactg
    40561 ttccgctaca ccgtgaagca cagtctgatt tttgccagcg ttatcaccct gctgcaggcg
    40621 tatgtgttta ccgggatgtt agtctcgtaa atcaataaag ccggatgaag gtaaatatca
    40681 cccggctttc catttgtctg tcgtttatct cgccagggaa ccgtttttac gattggcttc
    40741 tttatacgta gtctccgttt tacgatgcga attgtttaat atttatacaa cttggcttct
    40801 gtgctggtta taaagtaccc actttcatga gatcaatttt agtatgttat tacattgctt
    40861 catctgttgc tttcatgacg aatggcgttt tttctcttat ttatattttt cactagaaca
    40921 atgagtagat caaaattccc aaaactccca aaaacataag attattcgca aagctgattt
    40981 tgcagaataa tgagaacttt taagatttgt cgaggatcaa ttttaagcaa tttatctcca
    41041 cttgttacat aaaagatgcc gttaggttaa taagttaatc ttgctataac agtgttcgtt
    41101 actgcctatt aataaagata tttgggatag ggtaacgtgg atgaggattc gaaatcatcc
    41161 gtcttgcgga aagtgaaatg ttggcagatg ttaatattat gtatgtattt taactccaac
    41221 aacacaccaa tcattttgtt agaaataaga aaatttatgg ttaaataatt tggtgattat
    41281 gaagcattaa atcgtaatgc caaatattgt tcgctttgga gagtattatt gcctgtttgt
    41341 gtctgttgag cagaaaaatc atctgaattt gaatactttt attaggtgtg aataaatga
//
LOCUS       DAJTMX010000022        40005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTMX010000022 DAJTMX010000000
VERSION     DAJTMX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.022"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..155
                     /locus_tag="OO145_001167"
     CDS             <1..155
                     /locus_tag="OO145_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5466353.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO145_001168"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO145_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5466354.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO145_001169"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO145_001169"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5466355.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO145_001170"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO145_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO145_001171"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO145_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5466356.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO145_001172"
     CDS             complement(5626..7134)
                     /locus_tag="OO145_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5466357.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO145_001173"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO145_001173"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5466358.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO145_001174"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO145_001174"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5466359.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGLW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO145_001175"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO145_001175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5466360.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO145_001176"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO145_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5466361.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO145_001177"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO145_001177"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5466362.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO145_001178"
     CDS             complement(14283..15602)
                     /locus_tag="OO145_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5466363.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO145_001179"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO145_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5466364.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO145_001180"
     CDS             16699..17034
                     /locus_tag="OO145_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5466365.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO145_001181"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO145_001181"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5466366.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO145_001182"
     CDS             complement(17790..19172)
                     /locus_tag="OO145_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5466367.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO145_001183"
     CDS             complement(19358..20302)
                     /locus_tag="OO145_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5466368.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO145_001184"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO145_001184"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5466369.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO145_001185"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO145_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5466370.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO145_001186"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO145_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5466371.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO145_001187"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO145_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5466372.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO145_001188"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO145_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5466373.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO145_001189"
     CDS             complement(25947..26768)
                     /locus_tag="OO145_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5466374.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO145_001190"
     CDS             complement(26868..26951)
                     /locus_tag="OO145_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466375.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO145_001191"
     CDS             complement(27044..27379)
                     /locus_tag="OO145_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5466376.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO145_001192"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO145_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30065
                     /locus_tag="OO145_001193"
     CDS             29136..30065
                     /locus_tag="OO145_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5466377.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAKEPFVFFDPHVGTGLY
                     DDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAE
                     EDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30200..31420
                     /gene="mlc"
                     /locus_tag="OO145_001194"
                     /gene_synonym="dgsA"
     CDS             30200..31420
                     /gene="mlc"
                     /locus_tag="OO145_001194"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5466378.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31545..32240
                     /gene="bioD"
                     /locus_tag="OO145_001195"
     CDS             31545..32240
                     /gene="bioD"
                     /locus_tag="OO145_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5466379.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYISLA
                     MLRSVLAVDRVTV"
     gene            complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO145_001196"
     CDS             complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO145_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5466380.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO145_001197"
     CDS             complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO145_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5466381.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34301..35155)
                     /locus_tag="OO145_001198"
     CDS             complement(34301..35155)
                     /locus_tag="OO145_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5466382.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO145_001199"
     CDS             complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO145_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5466383.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35785..37823)
                     /locus_tag="OO145_001200"
                     /pseudo
     CDS             complement(35785..37823)
                     /locus_tag="OO145_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37884..38993)
                     /locus_tag="OO145_001201"
     CDS             complement(37884..38993)
                     /locus_tag="OO145_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5466384.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38976..>40005
                     /locus_tag="OO145_001202"
     CDS             38976..>40005
                     /locus_tag="OO145_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5466385.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10169 a         9703 c        10247 g         9886 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggctctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gaagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt
    29761 ttgatccgca cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc
    29821 atttgacgcc cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt
    29881 ccgccggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa
    29941 tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga
    30001 aacatcatga acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca
    30061 ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta
    30121 aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg
    30181 aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa
    30241 gcagaccaac gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat
    30301 cgatctttcc cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat
    30361 gctcgaagca cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc
    30421 ggcggtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg
    30481 cggggagatt ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca
    30541 ggaactggcg ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca
    30601 gttttttatc cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc
    30661 gggaattatt gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa
    30721 agagatgccg ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca
    30781 tgatatcagc gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga
    30841 tgtgattcag gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct
    30901 gctacacgca ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg
    30961 taaacgctgt tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat
    31021 tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc
    31081 gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat
    31141 cattatcggg gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa
    31201 cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt
    31261 catctcagac agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga
    31321 gagtactcag ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat
    31381 gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc
    31441 aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt
    31501 attttcctag tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct
    31561 ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag
    31621 cgttagcctc ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag
    31681 agacacccga agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg
    31741 aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc
    31801 acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag
    31861 tcgatcatgt cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc
    31921 cactctctga atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg
    31981 aaggttgcat taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc
    32041 tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg
    32101 atgtgctggg taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg
    32161 ccgaacagcg tgaactgggg caatacatca gcttagctat gctgcgcagt gtgctggcgg
    32221 tagatagagt cacggtgtaa cgtccgcgaa attaccgacg caattacgca ggcaatcaat
    32281 aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc
    32341 ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta
    32401 atttcttcgc catcagggaa ccataatccc aggctacgac catacaacat gccaatggca
    32461 agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc
    32521 agcacggcac acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg
    32581 gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa
    32641 agcagaccca cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc
    32701 acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc
    32761 agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt
    32821 acgttgtaga gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg
    32881 gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct
    32941 ataaataaac tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca
    33001 caggcgatcc ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg
    33061 gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact
    33121 accagcagcg aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt
    33181 tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag
    33241 cccatcagca acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag
    33301 gaggaaaggt ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac
    33361 tccagtagca gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg
    33421 actgttgcga taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga
    33481 tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac
    33541 cgcaaaagca gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc
    33601 gaatcgcgcg taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt
    33661 taaccgcgac aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt
    33721 cacccagtgc acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg
    33781 ttgaccacgg aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct
    33841 ccgccaacca cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct
    33901 tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc
    33961 caaacagcac agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc
    34021 acgggccgac aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa
    34081 aagccgtcac cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt
    34141 cactggtgag taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca
    34201 gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc
    34261 ctaacatcct gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc
    34321 ctactgtcat gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga
    34381 ggattagccc cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa
    34441 gccagcatcc aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat
    34501 ctggtaccag tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt
    34561 gcagcacaat cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg
    34621 tggtattaaa tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca
    34681 acaataccgt caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt
    34741 cgatttgata cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa
    34801 gtaaccagag tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata
    34861 gtccgccaac ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc
    34921 cgattcgatt cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa
    34981 taaacccgat gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt
    35041 ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc
    35101 attgacccaa tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac
    35161 acttcctccg gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta
    35221 ggtttgataa cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc
    35281 agagtgccgt gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg
    35341 caggattcga cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac
    35401 ttcgtcatgt gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg
    35461 cagtagcggc agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta
    35521 tgcattgcgc cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag
    35581 atggagagat aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg
    35641 ccgccagcat attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct
    35701 ttgcacgcca gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca
    35761 tattgtgtgg tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga
    35821 cgggtttccc tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc
    35881 gtgatcgacc cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt
    35941 aacgccaggc agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt
    36001 aaatacccgc acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat
    36061 ccacacctct tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt
    36121 acgcgctttg gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt
    36181 gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag
    36241 ataaggtctt cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc
    36301 gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc
    36361 acctcatggg tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac
    36421 cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg
    36481 gtcattttcg taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta
    36541 acaggattct cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca
    36601 cctgagttgc cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg
    36661 cgggacgttt gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca
    36721 ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg
    36781 atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag
    36841 gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca
    36901 caatatttgt cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc
    36961 gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg
    37021 gcagctgtgt cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg
    37081 gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt
    37141 ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca
    37201 ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg
    37261 agaaaacccc cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg
    37321 ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc
    37381 cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta
    37441 ccttcaccgc gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc
    37501 cgtcggcgga tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg
    37561 tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc
    37621 agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga
    37681 gcctctgccg cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg
    37741 agactgccaa gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc
    37801 gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg
    37861 ccagaattta tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc
    37921 gggtttccct tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca
    37981 tggtcgattt tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta
    38041 actccaggga gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta
    38101 aacacccgca ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc
    38161 cacacctcct gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta
    38221 cgggatttgt aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc
    38281 cagccttcaa aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt
    38341 tcccaggtac gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc
    38401 gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca
    38461 ttgggatctt tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc
    38521 ggatctttgg caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct
    38581 tctgtgaact tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag
    38641 taaatcggtt tacgttcgaa tttttcgctg gtgactggct ggaggaaaat gagatatccc
    38701 atgttaccgg cgtagtcgtt aggaataata tcttcctgct caacggtcat caggtcttcc
    38761 agcagaatgt cggcatattt tgccgatgag gtcataaagt tttcgatgac cacaatcatt
    38821 tcgcatttcg attcgtcctg cagaatttca tgcgttttgt tgatgtcaga atgctgatta
    38881 acgagggtat ttcccgcgta gttccagatg gactccattg ccagacggat ggatggactt
    38941 gaagtagttg actcaatgct acttaaggtc ctgatatgac tgacattaat catgataaca
    39001 ctgtatatcc atccagttct ttctctgggt ctccctatcg gtatcagcaa gtctacacct
    39061 caagccctga cacaaggctg acacaaatca atctactcag cgtagctaag cctcagttag
    39121 tccgcagagc caatggtcga tatactatcc gcttcagact caagggacag accacaccgt
    39181 ttctttcagt atcgacccgc agcacagaca ggagagtagc cacaatgcgt cagagagagc
    39241 ttgcagccac cgctaaggcc ttcatgttgg ataggcctga agtctcacta caggagctta
    39301 cagagcatct ccggtctatg gctgagcagt tcctgacaga cgctagtgac gattactgga
    39361 atggacttga ggtcgctact ctggtcgacg agaagtccaa cctgaaggag ctagccgcta
    39421 cgcaagcctt gagtctggac cagcagaagg gcataaggtt agcgctggaa gtcctgaccg
    39481 cagcacagca gagagtcgac actggtgata cctctggtct gattaagctg attgatgata
    39541 ataaccttac tgatgactca actataggcg actctacgtc aattctgaat aatgagcagg
    39601 gggatagacc ggcagttttc acacaggagc gccagtcatc tgtagttttt tcaagcctag
    39661 tgtcctcgct gctggctgag aaggtccaga ccctgaagac atccagctac aaggacctct
    39721 cgtcctcact caatactgtc tcccggttcc tgcctgaaga catggacctg atgtcccgct
    39781 ctgggtggct ggctgtcagg gactctatgc tggcttctga ggtgagacca tcgacaatca
    39841 acaagctgct gaccaaagcg aagatgtgtc tcgattatgg cctgatgaat gggcaacttg
    39901 agggccgcaa ccctattgaa cggatgaagc tgaccaagga tatcgactcc aagcgcagag
    39961 cgttcacaga cgaggagctg gagagacttc tggtccgtgt tgagt
//
LOCUS       DAJTMX010000023        38787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTMX010000023 DAJTMX010000000
VERSION     DAJTMX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.023"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO145_001203"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO145_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5466386.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YSSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            531..572
                     /gene="hisL"
                     /locus_tag="OO145_001204"
     CDS             531..572
                     /gene="hisL"
                     /locus_tag="OO145_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5466387.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="OO145_001205"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="OO145_001205"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5466388.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="OO145_001206"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="OO145_001206"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5466389.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="OO145_001207"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="OO145_001207"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5466390.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="OO145_001208"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="OO145_001208"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5466391.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="OO145_001209"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="OO145_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5466392.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="OO145_001210"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="OO145_001210"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5466393.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="OO145_001211"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="OO145_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5466394.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /locus_tag="OO145_001212"
     CDS             7140..7751
                     /locus_tag="OO145_001212"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5466395.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO145_001213"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO145_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5466396.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO145_001214"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO145_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO145_001215"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO145_001215"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5466397.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12304)
                     /locus_tag="OO145_001216"
     CDS             complement(11945..12304)
                     /locus_tag="OO145_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466398.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12301..12807)
                     /locus_tag="OO145_001217"
     CDS             complement(12301..12807)
                     /locus_tag="OO145_001217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466399.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12953..13516)
                     /locus_tag="OO145_001218"
     CDS             complement(12953..13516)
                     /locus_tag="OO145_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466400.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13723..14661)
                     /locus_tag="OO145_001219"
                     /pseudo
     CDS             complement(13723..14661)
                     /locus_tag="OO145_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14724..15098)
                     /locus_tag="OO145_001220"
     CDS             complement(14724..15098)
                     /locus_tag="OO145_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5466401.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO145_001221"
     CDS             complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO145_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5466402.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO145_001222"
     CDS             complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO145_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5466403.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17168..18058)
                     /locus_tag="OO145_001223"
     CDS             complement(17168..18058)
                     /locus_tag="OO145_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5466404.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO145_001224"
     CDS             complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO145_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5466405.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO145_001225"
     CDS             complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO145_001225"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5466406.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO145_001226"
     CDS             complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO145_001226"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5466407.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO145_001227"
     CDS             complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO145_001227"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5466408.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO145_001228"
     CDS             complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO145_001228"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5466409.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO145_001229"
     CDS             complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO145_001229"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5466410.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO145_001230"
     CDS             complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO145_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5466411.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO145_001231"
     CDS             complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO145_001231"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5466412.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIYMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO145_001232"
     CDS             complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO145_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5466413.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO145_001233"
     CDS             complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO145_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5466414.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO145_001234"
     CDS             complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO145_001234"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5466415.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO145_001235"
                     /gene_synonym="manB"
     CDS             complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO145_001235"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5466416.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO145_001236"
     CDS             complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO145_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5466417.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34324..35508)
                     /gene="wcaI"
                     /locus_tag="OO145_001237"
     CDS             complement(34324..35508)
                     /gene="wcaI"
                     /locus_tag="OO145_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5466418.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DK"
     gene            complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO145_001238"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO145_001238"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5466419.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO145_001239"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO145_001239"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5466420.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO145_001240"
     CDS             complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO145_001240"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5466421.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="OO145_001241"
     CDS             complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="OO145_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5466422.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38666..>38787)
                     /locus_tag="OO145_001242"
     CDS             complement(38666..>38787)
                     /locus_tag="OO145_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5466423.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10357 a         9712 c         8730 g         9988 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga agagtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa
    12541 tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac
    12601 atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg
    12661 tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa
    12721 tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca
    12781 ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt
    12841 tttcgctatc aatataacaa atatgatggc tccatatatg gcaccaaata gttttacgcc
    12901 acaagcaatt ccaataaaaa aaccacataa aaaaattaat tcattttgtg ttttattgtt
    12961 ttctgagcga atatatataa gaatactggc tgcagcaaaa caaagatagc gttaaatcaa
    13021 tatatgcata accaagatga tctttaagtg gattgatagt aaatagtagg attgtagcaa
    13081 taatgcctgg ataataagtt ttcgtaattt cccatgaaca gccaacaagt ccacaggcta
    13141 ttacaaaaaa acagatattt gctaaaaact gagctaacct tacatctcct aactgtaaac
    13201 ctaaagccat taaaagatct gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa
    13261 caacaattgc atggtgctca actaatgaac gagccaaagg taagtgatac atagtatcat
    13321 cccaatcacc gggagctcgc agggacgata acaatgtaac cacaaaaatc aaaacaagaa
    13381 ataaaataac aaacagcgta ctgaactcaa tattatttat tgccagccat ctaaattgct
    13441 gacgtttaat aatgcttaaa acccaacaca ccgtaaaaat taaaaagata gcaatcttat
    13501 taaattgccc gaccatgccc agaaagtaaa ttatcaaaat atttaaagac atccctatgc
    13561 ataggcaata aaaaataaag aaaatttgtt aagtcctgcc accccctcct taaaaaaaga
    13621 tgacaataca atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac
    13681 atacaaatac aacggcaatt caatcatagt aaaatactct atttacatta tcagtttata
    13741 gtttttttca gaaagataaa ataatgcctt catttcacgt ctagttgttg tatttgaatg
    13801 caaaataatg ccacatacaa atgccattat tgacgataac atgatggagg cagttaaaaa
    13861 ggcagtcgga aatcttctca ccaaacctgt atcaatataa tcaacaatga tgggcaatcc
    13921 taaaattata gacaaagcag caagtaaaac gccgataata ctaaaaaagt atagtggccg
    13981 ttcaagcgag taaagtttaa ttattgtttt cagaatcttg aacccatcac tccaagttga
    14041 tagtttgctc acactccctt cagatcgctc gccatatttt gtattaacct caccatactt
    14101 catacgtaat tctaaagcat gaattgtaag ctcagtttcg atttcaaatc cccttgatag
    14161 acatggaaaa gatttaacat agcgtctgga aaatacacga taccccgaca acatatcagt
    14221 aaaattccct ttaaagatag tctgaacagt accagtcaac aatttatttc ctcagcgatg
    14281 cccttttctg tatgtatttt gatctccatt ctcttgacga catcctacaa ccatatccaa
    14341 ttgatttttt atgagatggt taatcatttc tggagcagaa cttgcatcat aagtgtcatc
    14401 tccatcaacc attagataaa tatccgcatc tacatctgag aacatacgtc ttacgacatt
    14461 ccccttgcct ttcagaggaa cagagtgaac tgttgctcca gcatcctctg ccacttgtgc
    14521 tgtactgtca gtcgaattat tatcaaatac atatatagaa gcagttggaa tattcgtttt
    14581 gaaatcattt atgacttttg ctattgcctt ctgctcatta taacaaggaa taattacagc
    14641 gatattaaaa gagtgataca taagcccctc atataaacta tttagctaat gtgcaagtaa
    14701 ttgtgttttt aacaaatcgt ttactaacga aaaatgaaat attttgatgc gcaatatcct
    14761 aaaaccaaac taaaaagaga aaaggtgaac aatgtaaata ttggagataa agctaagaga
    14821 tcaaataaaa cgccgaataa ataactcatc gcccccataa aaaatataaa tataaaatat
    14881 ctagccttgg ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt
    14941 gctgcacata taaaaccaac aatgttactt cggccttgac caaaagctaa attattataa
    15001 cacgcataaa atacccccca atgtaaagca gtattgacaa ttccaattaa aaaataacta
    15061 aaaaaagaac tggtcaataa cttccctatt tttaacatcc tttattttgc tccagaagtg
    15121 aggttattac taatttggat attttctata gaaaataccc ataaaactac aacgcctata
    15181 ataaaaattc ccatgggtgt aaaaagaaat ggattagtta tagaaacggt aatactgaaa
    15241 aaaagtatta acgacatcat taatgatgtt tttgtactat ttaaaaaata gcctttcaaa
    15301 aagataagca acataggaac taatgatata aatacaccaa taacgcctgt tttctgaagt
    15361 atctcaagag gtactatttc aatccttaat cgatctaata taaatgaacc aaatcctctt
    15421 ccaaacaaga acgtcgctaa atcaacattt ttataataaa aatataaatc attaaatcta
    15481 acagagtcag aatcttcact gcgtgatccg accatgtaaa gcataattat aaatagaacg
    15541 ctgcccaata ttattataat agtatttaaa tatagcttat gtaatttaaa actggctaat
    15601 aacagtgata atgttgtaaa aagtaataca cctcttgatt ctgtcaataa tatcgcaagc
    15661 actattatga gacattttaa atattttttg tcgacaaaac tgataatcgc accgacacca
    15721 aaaaagtaga aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc
    15781 aatgataagt gttcataaat taaattaaaa tttaacaaac cgaaattcaa caaaattatg
    15841 tatgaaatat ataacagtga tccatataat gcaaatgcat taactatttt aataaaaatc
    15901 tcacatgaaa atacttttaa agtatatctt agtgtaaata gatatataag tgacagcata
    15961 aaaaggtatg gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg
    16021 acaaagaagc cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg
    16081 gtgattgaaa aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat
    16141 aataaatatc gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata
    16201 atcagttcaa tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg
    16261 tgatgttttt taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt
    16321 tttccgcctt aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc
    16381 caattgatta ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt
    16441 tcgataccac ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt
    16501 tgctcccact tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata
    16561 ccacgaataa tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc
    16621 ttgtgcagca tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt
    16681 tataacatat gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga
    16741 ctgagcctta tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga
    16801 ataacaattc gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata
    16861 ccaaatatcg tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt
    16921 aattaatcta taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt
    16981 tataattgag ggatatgata attgaaattc ttcacaaata tttaatgtat tatcagttga
    17041 taaatcatct gatataaaaa ttgagatgtc tacatctttt tgttcaataa tactttttaa
    17101 ttgttcctca atccagcatt cgccattata cgtagccata agtacagcta ctcttttttg
    17161 atcagaacta tgcattataa cgaccgcccc cagtaattcc tcttattcca tccagcgtag
    17221 ccaaaatatg ttttttcata tattcaattt tatttttttg agttgcgata attaaagtcg
    17281 tatgaattaa agaaaaaaca acttccctaa tagagagtaa aagaggcaca tgattttttc
    17341 taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag tgaattggta
    17401 tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt tctatatcag
    17461 gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct gtatcgacat
    17521 aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct tttgaaacca
    17581 tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca gtgacatgaa
    17641 ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt gacttatctc
    17701 tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag caggaaagtt
    17761 ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc ttctctgccc
    17821 ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct tcattcctac
    17881 caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag gtgaaattag
    17941 tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg atcagtgcat
    18001 tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta ctattcatta
    18061 tccaagtgac tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata
    18121 aagccattgt aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc
    18181 cagtaaccca tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc
    18241 acaatgaatc aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat
    18301 cattgccagc gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt
    18361 aatttgataa ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat
    18421 gattatcacc ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt
    18481 aaattcttta aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc
    18541 aggtattatt cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc
    18601 tgcccccata atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat
    18661 attacttact gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa
    18721 atgaaccccc gaaattttaa tatcattcct gacataatag gcagagacta aaatcgaaat
    18781 aactcttgca aatattaaac cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa
    18841 tatggccgga attcccgcga tgcaagagct tgatatagat ttttgaatat ttacaatgcc
    18901 aaatttttca tccccctcaa gaatggctga ccataattga tttagaataa ataatggaat
    18961 gcaaatagcc aacagtttaa atgctagttg tatatcacta tgttcaacac cagaaatttt
    19021 tagataatta acaattccat cagagaaaat caataataaa aaagcaccaa agcatgaaaa
    19081 tagcaccaaa aaagatgtac ttgttgaaat taccttggtt ctttcatgat gattatcacg
    19141 atgaattgca atttctctaa taacagagcg agtcaggcct acatcaaaaa ttccagcata
    19201 cccaacaagt gcaattgcaa ttgtataaac accgaaacct tcaggcccca aacctcgagc
    19261 gagaaatcct agcgagggga tagcaattaa agttggtatt gcatagccaa aaaggttcca
    19321 gacactattt tttattatgc tcattattac atttctttat catataaaat aatcagcatc
    19381 tacaaatttt tgaccattaa tatctttatc agaaagcaat ggattttgaa ccggccattc
    19441 tatatttata cttttgtcgc tccataaaat cccacgctct tgcatttttg aatagaaatt
    19501 atttgttttg tagacaaatt ctgcttcatc actaagaacg caaaacccat gagcgaatcc
    19561 ttccggtatc cataattgac gcttattctc cgccgacaaa tttacaccaa cccacttgcc
    19621 aaatgttggt gatgatttcc gaatatcaac agctacgtca aaaacttcac cgacaacaca
    19681 gcgtaccagt tttccttgag cataaggttc cagctgatag tgcagtccgc gtaacacacc
    19741 cttagttgat ttagaatggt tatcctgaac aaactcaacc tttcgtccta cagcctcttc
    19801 gaaaactttc tgattgaagc tttccataaa aaagccccgc tcatcaccaa atacttttgg
    19861 ttcaaaaatt agcacatcag gaatttcagt tttaattacg ttcattttat taataacctt
    19921 taatcatttt cagcagatac tgaccataag catttttctt cagcggctcg gctaatacct
    19981 tcacttgctc agcatcaata aagcctttac ggtgagcaat ttcttccggg caggaaactt
    20041 tcagcccctg acgctcttca attgttgcaa tgaagttgct tgcttcaata agactttgat
    20101 gtgtccccgt gtccagccat gcataaccac gccccatcat agcgacagac aaacgtcctt
    20161 ggtccatata aatacggtta atatcggtaa tttccagttc gccacgtgca gatggtttaa
    20221 ggtttttcgc catttctaca acgtcattgt catagaaata aagcccagta accgcatagt
    20281 tactttttgg ttcctgcggt ttttcttcca gactaattgc cgtaccgtta tcatcaaatt
    20341 ccaccacacc gtagcgctca ggatcattta cgtgataggc aaaaaccgtt gcgccactct
    20401 ctctgttaac agcggtatcc attaatttcg gcaggtcgtg accatagaag atattatcac
    20461 caagtaccag cgcacaatca tcaccaccta taaattcttc accaatgata aatgcttgcg
    20521 ccagaccatc cggactcggt tgtactttat attgcagatt aagcccccac tggcttccat
    20581 caccaagcaa ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc agaatatctc
    20641 gaatacccgc caacataagc gttgaaagcg gataataaat catcggttta tcataaatcg
    20701 gtagcagttg tttgctcaca gccattgtca caggataaag acgagtgccg gaaccaccag
    20761 ccagaataat acctttacgc gttttcattt caccattcct tttaattcat ctcgctctag
    20821 catcatgagc gagatgcaat aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc
    20881 atacgtttca cgcccacctg ccagtcaggc aagacaagcg caaagttctg ctgaaatttt
    20941 tcggtattga ggcgagaatt atggggacga cgtgctggtg tgggataggc cgttgtcggc
    21001 acggcattaa gtttgttaag agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc
    21061 agagcggcat aatcgtgcca ggttgttgta ccaccagcaa ccagatggta caaaccagca
    21121 acttctggtt tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca
    21181 gcccccgttg gtgcgccgaa ttgatcgttt attacagcca actcttcgcg ttcttttgcc
    21241 aggcgcaaca tcgttttggc aaagttattt cctttaccag cgtataccca gctggtacgg
    21301 aaaataagat gctttgcgca atgttcttgt aacgcttttt ccccagctag cttggtttca
    21361 ccgtagacat ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc accgttgcct
    21421 gggaaaacat aatcagtgga gtagtgtata acccaggctc cgacttcatt agctgctttt
    21481 gcaatcgctt cgacgcatgt cgcgttaagt aattgcgcga aattcggttc tgattccgct
    21541 ttgtctactg cagtgtgagc agcagcatta acaataacat cggggcgaat ttttttgacg
    21601 gtttcagcca caccttcggg gttgctgaaa tctccacaat aatcagtgga gtgaacatcg
    21661 agcgcaatca agttacccag aggagccaga gcacgctgca gttcccaacc cacctgccct
    21721 gttttgccga aaagcaggat attcattact gacggccctc atagttttgt tcaatccacg
    21781 actgataagt accgcttttc acattctcaa cccaatttgt attagccaga taccattcca
    21841 ccgttttacg aatcccgctc tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa
    21901 ttttatcggc atcaatggcg taacggcggt catgccccgg gcgatcggca acataagtaa
    21961 tttgctcacg ataagatttc tctttcggta caatctcatc caacaaatca caaatagtga
    22021 gcactacgtc gatgtttttc ttttcgttat gtccaccaat gttataagtt tcacccgctt
    22081 taccttcggt tacgacggta tataacgcac gagcatgatc ctctacatac aaccagtcgc
    22141 gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca ttcagaataa
    22201 ccaatggaat aagtttttcc gggaagtgat aaggaccata attgttcgag caattagtca
    22261 caatggttgg taaaccataa gtccgcttcc aggcacgtac caaatgatcg ctggaagctt
    22321 tagacgcaga atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg
    22381 cttcgttatt atttgcttca tccggatggg gtaagtcacc ataaacttca tcagtagaaa
    22441 tatgatgaaa gcggaaactt tttttctttt catcattcag agcagaccag taattgcgcg
    22501 ccgcttctaa aagaacataa gtccccacaa tattggtttc aataaatgcc gcagggcccg
    22561 ttattgaacg gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct ggctggtgct
    22621 gtgcgaaaat acgcgacatg gctacagcat cgcagatatc tgcatgctca aaagcataac
    22681 gttcagaatc agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga
    22741 cattaacaac actatcttgc gtattattta ttatgtgacg aacaacagca gaaccaataa
    22801 accctgcgcc accagtaaca agtatcttca cttttttatt ccatatagct atagagcatg
    22861 ctgtgaaata gactgctcca gatttgatta atagaggtat taatacacgc taccgcccct
    22921 ggcttaacag ctaccagtgc actgcatgca tctcaacgat gtggcgagcg ttagccactc
    22981 gcgccaaatc cgaaaaattc aaacgttaat tgtcttacca aacagccgta gaaacaagga
    23041 aaatcctgga aaaatttgaa taatatgagc tatgtaactt cttgttttta taggctttat
    23101 aaaaacggca acggcaggaa aattcaatga gcaaaactta tgccacgaat cttcactgcc
    23161 gttataattt tcttatcaac cgttacatcc ggtcagattt tcattattcg cttaacagct
    23221 tctcaatacc tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa
    23281 acgcctgcat atagcccatt tttttgccgc agtcgtaact gtcgccggtc atcagcattg
    23341 catcaacgga ttgttttttc gccagctcgg caatagcatc agtcagctga atacgtcccc
    23401 atgcaccagg ctgagtacgt tccagttccg gccaaatatc ggcagaaagc acatagcgac
    23461 ctacggccat gatgtctgag tccagcgtct gcggctgatc cggtttttcg ataaattcaa
    23521 caatgcggct gactttaccc tcacggtcca gcggctcttt agtctggatg acggagtatt
    23581 cagagaggtc acccggcata cgttttgcca gcacctggct gcggcccgtt tcgttgaaac
    23641 gtgcaatcat ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa
    23701 cgtctggcag taccacgaca aatgggttgt caccaatggc aggtcgcgca cacaaaatgg
    23761 agtggcctaa acctaaaggt tcgccctgac gcacgttcat aatgatcacg cccggcggac
    23821 agatggactg tacttccgcc agcagttgac gcttcacgcg ctgctcaagg agtgattcta
    23881 actcataaga ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga gttaccagga
    23941 ggatttcttt gatccctgca gccacaatct cgtcaacaat gtactgaatc attggcttgt
    24001 cgacgattgg tagcatctct ttgggtatcg ccttagtggc aggcaacata tgcatcccga
    24061 gacccgctac aggaataact gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg
    24121 ttgctgaatt atagcttgtt cgattttttt cgccagcatc aattaccctg aattgattac
    24181 tgaattactt gtgatgttac gccgcttcgt tgtggattgc agtagcattg ttcctaagta
    24241 tggctccatt tttccaggaa tggtcgcaaa tctactccct ccgttccggc aatctaaagt
    24301 taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg gagctttgcc
    24361 ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt ttcgccataa
    24421 agacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgctggt ccaactgagg
    24481 attcctgcat cacattgata tttcgcataa aaagatgttg cggcttgttg tgtaactcca
    24541 gcgaggcacg cttcatctca atgttagtta gcgacacaaa ggagacggca ttcccggcgg
    24601 agatttggat gccgcgcaat ttataagcaa gatgggtatt atccagttga atattattca
    24661 ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag ccgattaaca
    24721 tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa ccgtaaatag
    24781 cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata ttgcgggcat
    24841 tgatattacg aataacaaaa tgtttaccat tttcaacatg gatcaactgc cgacaatccg
    24901 atcccgtgat attcgccacg acaaagtttt tcactgcctg gtcttccggg tagttgttat
    24961 cataagtgct tcccgcaagg cctatgccga tgccccagtt gattttgccg ttggtacagt
    25021 tgatacgctc gatgacatgg tcagatatca aaatatcact gtcgttaatt gccacgttcc
    25081 attcaatggc gtcgccctgt aagtcgctga acttacagtt ggtaatgttg gcaccgataa
    25141 tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctgaca gtgaggttat
    25201 cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc aatataaatc tgcattaccg
    25261 ggccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc acatccaggg
    25321 taatgttatg cataccgccg ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa
    25381 atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg ccgggaggga
    25441 tgaagatgcc ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc
    25501 cttcgctgaa ggcttgttta aatgaggcga tccagtcatg tgggttgtag tcgttaatgt
    25561 taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg
    25621 agcttgccgt caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt tctcctctat
    25681 aaagcctgca gcaagctggc gagttctcga ttaatcacct gctggttaaa atcgtgttcg
    25741 actttttcgc acgcacgttt gacgaccgga gccagttcgt cggtatccag ttggctaaac
    25801 gccgccagac gttgtgccag tgcgcgagca tcgttctcag gcagcagcca gccggattta
    25861 tcggcctcca ccagttcagg aattccgcta tgcagagtag aaaccaccgg aataccgacc
    25921 gccatcgctt ccattagcgc caccggaatg ccttccatat cgccatccgc gcccgttacc
    25981 gatggcaaca ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg gctcggttta
    26041 aagcccggca tctctaccac atcttccagt tgatattgtt cgatgatggt gcgcaggcgt
    26101 cgttcccaca ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg ctctttcaac
    26161 tgacggcagg cttcaatcgc cacatgcagg ccttttttct cggttaagcg tgcgacagat
    26221 atgatttcca gcggcgttgc gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc
    26281 acgcccatac gcgatacggc gattttttct ctggggcagc ccattttttg cagccttccg
    26341 gcccacagat cgcttatcgg taacatcagg tcgccacggc gaaacagttg ttgatattcg
    26401 ggagtgtagt ggttgagcac ttcccgactg gagatatcaa taccgtggaa gatggtggca
    26461 attttgccgc gaatgatacc cagttcgcgt agttttgctg cggttacccc cgccgggcca
    26521 aaatgagcga tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac
    26581 aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt
    26641 ttacgatgaa tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc tactttgccc
    26701 gcaggttcgt cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg
    26761 tgggtgttct ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa gcccatataa
    26821 ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcgggaa tttcaataag
    26881 aagaagccga ccttcatttc acctccccga tgcgctcaag cacagactgc accatctgca
    26941 ttcccgtctg acgttcgcga ctgacggctt cgttaaatcg agcgttcagc accggaagct
    27001 ggcctaaggt atccgcaacc atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa
    27061 tcgccatctc tggcagtccc agctgctgca taatcccggc ggatttatgt tcatagttga
    27121 tggcaatcgc cggagtagca aagttcattg agataatggc ggagtgcagg cgcgtaccga
    27181 cggtgagttc acaggctccg agaattttgc ccatttccag atcgttgagt tcatccatca
    27241 ctacgtggta acgggcagga tcgctgatgt gctggcgcag attgagcgcc accatgcggt
    27301 cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatcacc tgatagcctt
    27361 catcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgt tgagtggtgc
    27421 cgagacgttt gtcgaacggt gccagttcgc gcagggtaat tgccactgtt ttctgttgtg
    27481 cggcaacgtc cagccagtgt tgaacggcat agctggcggt gaagtcttcg gtgtggtgat
    27541 cgaccagcca cgcggtatcg acgccatgtt ccacttttgc ggtggtgata tttctgcgtt
    27601 tcatcaaatc aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat
    27661 agttcgccag ttggttaaat tgctcatcct ggaaggggcc gacgctgtga ccaatcataa
    27721 acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga
    27781 gatcgacaaa aaacgatccg ccgacctgaa taatggcgtc gtaacctgac agcaggcgca
    27841 cgaagtcggt gaatccctgg gcgatggcga tattgcgcag cttgccagtg tcggtgacgc
    27901 gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaatg cgcccgacaa
    27961 cgcccgccgc gctattgtgt tgtgtcatct gcaggaacag cggatcgccc attaccgggc
    28021 ggttgagcag ccaggaagaa ctgaccggat agcggctcat cacgtccact tcggtatgtg
    28081 gattgaggat gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc
    28141 cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt tgatgccagg
    28201 tgaggctgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt
    28261 atcaggccta caggtcccga gcacagaacc gtaggacgga taaggcgttt tacgccgcat
    28321 ccggcaaccg ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa gcattttcat
    28381 tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc gtgacagcac
    28441 aatcatcacc acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag
    28501 cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg tcggcaacga
    28561 gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac caagaaccgg
    28621 tttaatcatc acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaaaccgag
    28681 cgtgacgccg attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa
    28741 tgttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga
    28801 accaatcggg ttacccacgg agcgcagcag acccacaata cacagcaatt gcagcaccgg
    28861 aataatgctg ttccactttt cgccgaatac cagcggtacg aagttattcg acaccaccat
    28921 cagccccagc agcgccggaa agttgataat ccccactatc gacagcagct tgtagaagtt
    28981 aacacgcagc ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca acacgcgggt
    29041 gatgattggg ttcagcttca tcggcggtac gacggccacg ttgtacgcca ggttgtatcc
    29101 ccctgccacg cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag
    29161 atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga
    29221 cgccagcgag aagtgcagac cggggcaata aattttgcgt ccaaagtagc caaacagcag
    29281 tgttctcacc gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa
    29341 atgggcgcta actaccgtaa aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc
    29401 gattttgttg aactccagct ccttctgcat caacgcgcgg aactgttgcc cgtgggggat
    29461 caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcagcac
    29521 gtcgccgatg acatcactca acaaaaacac cgccacacat accacaagcc ccagcccgac
    29581 gttcagccag tacagcgtgg tgagttcaag gtgactgatt tcttttcgct gaataatcga
    29641 gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacagt
    29701 cagcaggccg aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc
    29761 gaggccgatg atgatcaccg tggcaatagc cgaccacttc gcgccgctga tggttttttc
    29821 acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa
    29881 cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg aactcgacgc
    29941 gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc
    30001 cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc tggcgatact
    30061 gttcgttatg cgctactgcg tgcggacgtg gaccgacaat cgacatcccc ccggtcagca
    30121 cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc cccactttag
    30181 tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc atcactttca
    30241 tggaacggaa cttccacact ttgatcggct tgccatccat accatagcga gtctggcgga
    30301 aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc aatacagcac agcaccgggg
    30361 agatcagcag cagaataagc gtcgccagca caatgtcttc cgcacgtttt agcaggcggt
    30421 taacccccga aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc atctcttcga
    30481 gacgtgaatg gagaatgttg aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg
    30541 tatccgccag ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga
    30601 tatagacgtt atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg
    30661 cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta aacgcccacc acttcaaacc
    30721 ctaaccacgg ctgattacgg aaactctcca tcagcatttg tccggcggct aaatcccccg
    30781 ctacggcgac catccgcttg ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa
    30841 tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt
    30901 tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta
    30961 aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc
    31021 gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc agcgccacca
    31081 acaggtgcat gtagaggaat gacagtccgc tgacttcgca aaccagccat agcccggcaa
    31141 acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg
    31201 ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag
    31261 tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg
    31321 cttccatcag cggcacatca ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat
    31381 tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat
    31441 ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt
    31501 gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca
    31561 gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca
    31621 tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt
    31681 agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatta
    31741 ccggcgtgcc gcctgcagca gtcaccacat caatagtgtt ccaagagaga cgcggatcat
    31801 ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt
    31861 agtagccctc gataaactgc cctttttcgt caaacagaaa acagcggtca aaatcgccat
    31921 caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac
    31981 attccggcag cagcgggtta ggaataccgt tggggaaatt gccgtccggt gtgttgtgta
    32041 ctttgattaa ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca
    32101 ccggacccgc tgcgccattc ccggagttga tcaccagctt gagcggcgtg agatttttga
    32161 cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc
    32221 gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga cgctggacgt
    32281 cgcgcagtcc ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt
    32341 tgtaatccat cggattatgg ctggcagtaa cttcaatgcc gccatccacg ccgagatgga
    32401 acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg
    32461 catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga cggacatcac
    32521 cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg
    32581 cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg
    32641 ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca
    32701 attttatttt tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc
    32761 cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc
    32821 cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca
    32881 catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc
    32941 ccgggttttc caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa
    33001 gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt
    33061 ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt
    33121 tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt
    33181 cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca
    33241 catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca
    33301 gatctttcac cccgacggtg gtgaccaggc ctgattcggc atacacatag ctgttttcag
    33361 ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct
    33421 cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag
    33481 catctgccgt aggttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg
    33541 cttcttcatc cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc
    33601 aggcatcgag gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga
    33661 acaggaacat gccgctgttc cagtaatatt cgccgcttgc tacataggcc tgggcggttt
    33721 ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcaccca
    33781 cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc
    33841 caaaggtcac cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga
    33901 acgcgtcttc atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct
    33961 ccgggctatg acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc
    34021 ctgccggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga
    34081 caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca
    34141 ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg
    34201 ggtaaagtac gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg
    34261 gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac
    34321 gttctattta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag
    34381 caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac
    34441 cgcaataccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat
    34501 caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc
    34561 cgcgccgcgt ttttgcacca ccagatggca atcgcccatc ttcagcagtg caggtaaagc
    34621 gtcatacgat tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt
    34681 ttccagccgc gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcgcg
    34741 caggagatcg gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga
    34801 gtaaagaatg atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc
    34861 ggcgtctgca acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt
    34921 atccgccgcc acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac
    34981 gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc
    35041 gcctttgcct tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat
    35101 atgcagcacg gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa
    35161 cagcgttggc actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg
    35221 aaagaaactg ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg
    35281 ttttggcacg tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg
    35341 ccaggcggaa tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac
    35401 ccgcacctca tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat
    35461 gccggttaac tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact
    35521 ccggcacgct tctcagcgag aaaataggct cggctgttag cgtgaacatc gttactggcg
    35581 agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc
    35641 tcttcttccg ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg
    35701 ccggagaagt tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc
    35761 ggcagacgca gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg
    35821 tctttctgca cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt
    35881 ttgccaagca gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt
    35941 gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc
    36001 ggtcttgatt ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt
    36061 cgtgatacca gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat
    36121 ccggtttgct ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt
    36181 gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg
    36241 gttgggtatt ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg
    36301 ccatatcatc gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt
    36361 ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg
    36421 agttactcgg gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt
    36481 aatcgcgtcc atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa
    36541 tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg
    36601 gctgttttgc cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt
    36661 cgttctgatg cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat
    36721 ccgccggata ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct
    36781 ggtcaatgcg ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt
    36841 catcgcgggt acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg
    36901 cagaaccgac catcccacaa tggccagcga taaaaatgcg ttgtttactc atgcttatga
    36961 ctccagcgcg atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc
    37021 ttcgaggtca ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca
    37081 gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg
    37141 gaagtaacgc ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg
    37201 cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt
    37261 gatgcccagc tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt
    37321 cgcgataacg aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta
    37381 gtctttggcg tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc
    37441 cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc
    37501 gcggcgcggg gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc
    37561 gcggtagtta acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg
    37621 gtagaacggc gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt
    37681 ggaagcctga tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag
    37741 gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc
    37801 aacgtggctc attgcgccta ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg
    37861 cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt
    37921 gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt
    37981 aataccatgc acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg
    38041 tccggttaca ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa
    38101 tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat
    38161 ttgccggaag cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg
    38221 tgacgcctgg ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag
    38281 gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac
    38341 ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga
    38401 cgtcatcgcc aacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta
    38461 ctgacggacg aataactacg ttttttccta tttttgctcc gaataaacgt aataaaaaag
    38521 cccgccagcg atacaatact tgtggcgacc aggcaaatat tgttgcctgt actgcccacc
    38581 ataattgcac tttaatagcg ttgccgcccc ggaacccttt cggcaccgag aaaccgctta
    38641 aatcttgcat tggttttcct tatattcaga ctttgttata taaggcttta gtcttacctg
    38701 tcgtacgttg gcgtaaatgc caggataatt ctgcccagaa gccaggtaca tgtaatatct
    38761 gccgttggac ttttttggcg tcagcac
//
LOCUS       DAJTMX010000024        37627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTMX010000024 DAJTMX010000000
VERSION     DAJTMX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.024"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..175)
                     /locus_tag="OO145_001243"
     CDS             complement(<1..175)
                     /locus_tag="OO145_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5466424.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO145_001244"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO145_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5466425.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO145_001245"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO145_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5466426.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO145_001246"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO145_001246"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5466427.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO145_001247"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO145_001247"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5466428.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO145_001248"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO145_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5466429.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO145_001249"
     CDS             complement(5179..5421)
                     /locus_tag="OO145_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5466430.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO145_001250"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO145_001250"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5466431.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO145_001251"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO145_001251"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5466432.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO145_001252"
     CDS             complement(8338..9312)
                     /locus_tag="OO145_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5466433.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO145_001253"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO145_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5466434.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO145_001254"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO145_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5466435.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO145_001255"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO145_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5466436.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO145_001256"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO145_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5466437.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO145_001257"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO145_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5466438.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO145_001258"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO145_001258"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5466439.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO145_001259"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO145_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5466440.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTVVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO145_001260"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO145_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5466441.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO145_001261"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO145_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5466442.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO145_001262"
     CDS             23179..23382
                     /locus_tag="OO145_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5466443.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO145_001263"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO145_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5466444.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO145_001264"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO145_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5466445.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO145_001265"
     CDS             26387..26659
                     /locus_tag="OO145_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5466446.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO145_001266"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO145_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5466447.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO145_001267"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO145_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5466448.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO145_001268"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO145_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5466449.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO145_001269"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO145_001269"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5466450.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO145_001270"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO145_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO145_001271"
     CDS             complement(31906..32304)
                     /locus_tag="OO145_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5466451.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO145_001272"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO145_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5466452.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="OO145_001273"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="OO145_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5466453.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="OO145_001274"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="OO145_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5466454.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="OO145_001275"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="OO145_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5466455.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="OO145_001276"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="OO145_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5466456.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO145_001277"
     CDS             complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO145_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5466457.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGDIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9116 a         9412 c         9899 g         9200 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg tcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aacagcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaatatcg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gactgcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag
    37321 caagctcacc atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgcccac
    37381 cgccataatc gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt
    37441 ataaatgtcg acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc
    37501 taaccagact ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt
    37561 gtagtttttc aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc
    37621 gaacatt
//
LOCUS       DAJTMX010000025        36577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTMX010000025 DAJTMX010000000
VERSION     DAJTMX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.025"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(156..1391)
                     /locus_tag="OO145_001278"
     CDS             complement(156..1391)
                     /locus_tag="OO145_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5466458.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO145_001279"
     tRNA            complement(1538..1614)
                     /locus_tag="OO145_001279"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO145_001280"
     tRNA            complement(1657..1743)
                     /locus_tag="OO145_001280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO145_001281"
     tRNA            complement(1764..1839)
                     /locus_tag="OO145_001281"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO145_001282"
     tRNA            complement(1898..1974)
                     /locus_tag="OO145_001282"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO145_001283"
     CDS             complement(2077..3462)
                     /locus_tag="OO145_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5466459.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO145_001284"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO145_001284"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5466460.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQESFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO145_001285"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO145_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5466461.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO145_001286"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO145_001286"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5466462.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO145_001287"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO145_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5466463.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO145_001288"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO145_001288"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5466464.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO145_001289"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO145_001289"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5466465.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO145_001290"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO145_001290"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5466466.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO145_001291"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO145_001291"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5466467.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO145_001292"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO145_001292"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5466468.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO145_001293"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO145_001293"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5466469.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO145_001294"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO145_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5466470.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO145_001295"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO145_001295"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5466471.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO145_001296"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO145_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5466472.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO145_001297"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO145_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5466473.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO145_001298"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO145_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5466474.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO145_001299"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO145_001299"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5466475.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO145_001300"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO145_001300"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5466476.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO145_001301"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO145_001301"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5466477.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO145_001302"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO145_001302"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5466478.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO145_001303"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO145_001303"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5466479.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO145_001304"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO145_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5466480.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO145_001305"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO145_001305"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5466481.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO145_001306"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO145_001306"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5466482.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO145_001307"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO145_001307"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5466483.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO145_001308"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO145_001308"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5466484.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO145_001309"
     CDS             complement(31267..32913)
                     /locus_tag="OO145_001309"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5466485.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO145_001310"
     CDS             complement(32916..32966)
                     /locus_tag="OO145_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466486.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO145_001311"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO145_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5466487.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="OO145_001312"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="OO145_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO145_001313"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO145_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5466488.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="OO145_001314"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="OO145_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5466489.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="OO145_001315"
     tRNA            complement(36398..36473)
                     /locus_tag="OO145_001315"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="OO145_001316"
     tRNA            complement(36482..36558)
                     /locus_tag="OO145_001316"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8938 a        10011 c         9214 g         8414 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaagc tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccaccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaacc
//
LOCUS       DAJTMX010000026        35224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTMX010000026 DAJTMX010000000
VERSION     DAJTMX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.026"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO145_001317"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO145_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5466490.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO145_001318"
     CDS             1207..1869
                     /locus_tag="OO145_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5466491.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO145_001319"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO145_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5466492.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO145_001320"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO145_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO145_001321"
     CDS             2795..3100
                     /locus_tag="OO145_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5466493.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO145_001322"
     CDS             3103..3507
                     /locus_tag="OO145_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5466494.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO145_001323"
     CDS             3497..3796
                     /locus_tag="OO145_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5466495.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO145_001324"
     CDS             3982..4278
                     /locus_tag="OO145_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5466496.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO145_001325"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO145_001325"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5466497.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO145_001326"
     CDS             5627..5995
                     /locus_tag="OO145_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5466498.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO145_001327"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO145_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5466499.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO145_001328"
     CDS             7203..7904
                     /locus_tag="OO145_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5466500.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO145_001329"
     CDS             7927..8091
                     /locus_tag="OO145_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466501.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO145_001330"
     CDS             complement(8303..9613)
                     /locus_tag="OO145_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5466502.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO145_001331"
     CDS             complement(9641..10972)
                     /locus_tag="OO145_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5466503.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO145_001332"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO145_001332"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5466504.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO145_001333"
     CDS             complement(12683..13072)
                     /locus_tag="OO145_001333"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5466505.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO145_001334"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO145_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5466506.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO145_001335"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO145_001335"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5466507.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO145_001336"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO145_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5466508.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO145_001337"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO145_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5466509.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO145_001338"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO145_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5466510.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OO145_001339"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OO145_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5466511.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQANFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OO145_001340"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OO145_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OO145_001341"
     CDS             21379..21726
                     /locus_tag="OO145_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466512.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OO145_001342"
     CDS             21895..22545
                     /locus_tag="OO145_001342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466513.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(23166..23542)
                     /gene="rnpB"
                     /locus_tag="OO145_001343"
     ncRNA           complement(23166..23542)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO145_001343"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23575..24720)
                     /gene="garK"
                     /locus_tag="OO145_001344"
     CDS             complement(23575..24720)
                     /gene="garK"
                     /locus_tag="OO145_001344"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5466514.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24817..25707)
                     /gene="garR"
                     /locus_tag="OO145_001345"
     CDS             complement(24817..25707)
                     /gene="garR"
                     /locus_tag="OO145_001345"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5466515.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25737..26507)
                     /gene="garL"
                     /locus_tag="OO145_001346"
                     /pseudo
     CDS             complement(25737..26507)
                     /gene="garL"
                     /locus_tag="OO145_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26523..27857)
                     /gene="garP"
                     /locus_tag="OO145_001347"
     CDS             complement(26523..27857)
                     /gene="garP"
                     /locus_tag="OO145_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5466516.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28232..29803
                     /gene="garD"
                     /locus_tag="OO145_001348"
     CDS             28232..29803
                     /gene="garD"
                     /locus_tag="OO145_001348"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5466517.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29855..30679)
                     /gene="agaR"
                     /locus_tag="OO145_001349"
     CDS             complement(29855..30679)
                     /gene="agaR"
                     /locus_tag="OO145_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5466518.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30928..32208
                     /gene="kbaZ"
                     /locus_tag="OO145_001350"
     CDS             30928..32208
                     /gene="kbaZ"
                     /locus_tag="OO145_001350"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5466519.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32231..32704
                     /gene="agaV"
                     /locus_tag="OO145_001351"
     CDS             32231..32704
                     /gene="agaV"
                     /locus_tag="OO145_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5466520.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32715..33494
                     /locus_tag="OO145_001352"
     CDS             32715..33494
                     /locus_tag="OO145_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5466521.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33484..34293
                     /locus_tag="OO145_001353"
     CDS             33484..34293
                     /locus_tag="OO145_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5466522.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34380..34814
                     /locus_tag="OO145_001354"
     CDS             34380..34814
                     /locus_tag="OO145_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5466523.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34811..>35224
                     /locus_tag="OO145_001355"
     CDS             34811..>35224
                     /locus_tag="OO145_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5466524.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9119 a         9094 c         8415 g         8596 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaatttt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgcc tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcgggttttt acgtatatca ggtgaaactg accgataagc cgctttcttt tgggtatagc
    22981 gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt
    23041 tttacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agcgtcgtgg
    23101 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
    23161 aaaacaggtg aaactgaccg ataagccggg ttctgtcgtg gacagtcatt catctaggcc
    23221 agcaatcgct cactggctca agcagcctac ccgggttcag tacgggccgt accttatgaa
    23281 cccctatttg gccttgctcc gggtggagtt taccgtgcca cggactgtta ccagccgcgc
    23341 ggtgcgctct taccgcaccc tttcaccctt acctgatccc gcttgcacgg gccatcggcg
    23401 gtttgctctc tgttgcactg gtcgtgggtt tcccccccag gcgttacctg gcaccctgcc
    23461 ctatggagcc cggactttcc tcccctccac ccgtctcccc cgaagaggac gacgacgaag
    23521 cggcgactgt ctggtcagct tcggcgcgca gtatagaggg tttgcgcgcc cttgtcaccc
    23581 cgtgttgcgc attccaatcg ccagtgtcgc ggcaatatta cgtgaagcac ggcagatatt
    23641 gtcataagcc ccgcggaatg cttcgtccaa cgtacctatg ctggtcaata cgctgaagac
    23701 cgcatcaatg ccatgctgat gtacaacgcc aacatcatcg gtcaggctac ccgcaatgcc
    23761 aatcaccggt ttatggtact tcttcgccac gtttgcgacg ccaatcggta ccttgccgtg
    23821 aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat cgtgaatatg
    23881 ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt ccgcaccaag
    23941 aaacgccatc agcgccgcac ccataccgcc cgcagctcct gcgccgggga catcttttac
    24001 atcaacatgc agcgcttttt taatgacatc ggcatagtga gagaggttat tgtccagctc
    24061 aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg cgccgttatc
    24121 gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt ttaagcgcgg
    24181 atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc caaaaccaat
    24241 ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc ctgcgccgcc
    24301 atcatttgta gcgctaccgc caatgccgat aataatgttt gtcgcgccgc tctccagcgc
    24361 ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc gtttttccgc
    24421 aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt tgccatcgcc
    24481 ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc aggcgtgacg
    24541 ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac cgtcggcaac
    24601 cggaatagaa acgtactgtg catcaggaaa aatttcccga aatccttttt ctatcgcctg
    24661 cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta cgattttcat
    24721 acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc tggattccag
    24781 gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt cgactttcgc
    24841 cagtttttcg tagtagcaca ccagggcgct atgatccgcc gttcctaaac catctgctcg
    24901 cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgcgcgc cgacgccgtg
    24961 ggaagtatcc agcgcatttg ccagatcctt aatatgcaga tcaatacgga agcccggctt
    25021 gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc ccgccagtcc
    25081 accgcgaatt gcctgataca ccaggtccgg gttaacgccc gctttagttg ccagcgttaa
    25141 cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgattcgcca gtttggtgac
    25201 gttacctgca ccaattgccc cggtatgcac cacggaaccc gccattgctt tcatcaaatc
    25261 atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg taccgtcgat
    25321 ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt tcgctttcag
    25381 cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga tcaataccgt
    25441 acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt tcacatgagg
    25501 ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt tagccgtaga
    25561 ggctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac ggtcagcaac
    25621 caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca taatccccag
    25681 gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg tggtggttat
    25741 tttttaaagg tatcagccag tttatgagtg gcagagcgga agacgccgag atcgctgccg
    25801 acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc gaccggcgcg
    25861 aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg aattgctttt
    25921 tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag atcgctgggg
    25981 ccgacgaaga tgccgtctac gccttcggta gcggcaatgg catcgacgtt atctacgccc
    26041 tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc gaaataatcc
    26101 gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat gccttccggt
    26161 gggtaacggg ttgatgccac cgcctgctct gcttcctctt ttgtttctac aaaaggaatc
    26221 aggaagttat agaaaccgat atccagaaga cgcttaataa tttccggctc gttggtcggc
    26281 actcacacta ctggcgcgct ggcgctgcct ttcaaggcca ttaactgcgg aataaacgtg
    26341 gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc agccaaacca
    26401 agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg tacctgtttc
    26461 gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc ttataccttt
    26521 gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta gcagaccatc
    26581 gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg cagttcactt
    26641 accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa gacgttaaag
    26701 acgccgccac agaggccaac aatctctttc ggcgcggtgt cagaaatcac cggccagccc
    26761 agagcaccaa atcctttgcc aaaaaaagcc agcgccatca gcatgaccac cagcgtggtg
    26821 ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag cacaatcggt
    26881 agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga gaagacacct
    26941 cccagcacgc cgcccgcaaa accacacagt gccggaatcg aggcgaccag acccactttc
    27001 agaatcgaca tgcctttttc ctgcaccaga taaatcggga accaggtgag gaagaaccag
    27061 gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg gttagagagc
    27121 aattgcttga tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg ctttttgtgg
    27181 tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttcctc agacatacgt
    27241 gggtgatctg tcgggttatg aatcaacttg atccacagcg ccgtcagcac aaaacaaatc
    27301 acccccataa cggtaaagac gtgctcccat ccccaggcga aagtcagcca gccaagcaga
    27361 ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa agatggcgga ggcagtacca
    27421 cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa ggatggagct
    27481 tccgagaatc ccagcataaa gcgcataaag aacatggaga tccctgccca agccagcggg
    27541 aacatatcaa caaagccttg caggaaggtg aacagcgacc agaaaaagag gctgtaggtg
    27601 taaacttttt tcgagccaaa cttatcaagc aaccagccgc cggggatttg catcagcaag
    27661 taggcccagc caaaagcgga gaagatgtaa cccatcgaaa ccgcacttaa ctgcaactct
    27721 tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt aacggcggta
    27781 acaataaaaa taatcagtaa tattaaatag cgggtatgca cacctttcct tttttcgtca
    27841 acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta ttattatggg
    27901 gaaggtgtta tttataagtt tcatttatgc cgtaacgaca atgaactcgg gaattagtat
    27961 aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac tttttaaata
    28021 ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg ctttaccttg
    28081 ccgggcggca ctgcaatccc taaaatgatt gacattgatc acatttctgc gtttaaactc
    28141 ctgacattct tatttcaccc aatgaaatca tttattttta aatgagacca ggtcctcatt
    28201 ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca gacaagaaac
    28261 gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta ttgttaatga
    28321 taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg aacatattcc
    28381 ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta ttcgttatgg
    28441 cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg acgaatcaat
    28501 ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca aagtcccgga
    28561 acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg atggcagcgt
    28621 gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag gcgtggtgga
    28681 ctatgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt
    28741 ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg cagcagttgt
    28801 acctattcgt accattcaca atatttcgct gaaccctaac tttggcggcg aagtaatggt
    28861 gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa cggatgatgt
    28921 gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg
    28981 ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac aaaaactgaa
    29041 tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc attgcggtgg
    29101 cagcgatgcg ttttctggcg taacggcaaa cccggcggtt ggctatgcgt ctgatctact
    29161 ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg gaagtgcgtg acgcgatcca
    29221 tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga
    29281 gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc cttcgccggg
    29341 caacaagaaa ggcggtctgg caaacgtggt agagaaagcg ctcggctcca ttgctaaatc
    29401 gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt
    29461 aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg tggcttcggg
    29521 tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga tggcggtacc
    29581 cgtcattaaa atggcacccc gcaccgagct ggcgaaccgc tggtttgatt taatggatat
    29641 taatgcgggc accatcgcta ccggcgaaga aactattgaa gaggagggct ggaagttgtt
    29701 ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc aatggggact
    29761 gcataaccag ctggcggtgt ttaacccagc accggtgacc tgataattca attcgtatta
    29821 tgcccgatag cacacgttta tcgggcatat tgagtcacag cgaagacgcg tgatccccga
    29881 ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt
    29941 catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat
    30001 tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac atccgacggc
    30061 ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta
    30121 caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt
    30181 aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg
    30241 cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtact
    30301 tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc acccgatgac
    30361 ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagtg
    30421 cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc
    30481 caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg
    30541 tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc
    30601 gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc ttctcacctg
    30661 aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc
    30721 ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattggc gcaagctact
    30781 ctcgtttcag tgactttcat tacgtttctt ttgtgaatca gatcagaaaa ctattatctt
    30841 tcgttttatt tttatctcac catggcgcag tatcaattga aacaaaacga aagatcaata
    30901 tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac
    30961 aaagcgggca aaacaaatgc aatttatgcc gtttgttccg cacatccgct ggtgctggag
    31021 gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat
    31081 caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt
    31141 tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc tgattctggg tggtgaccat
    31201 ctggggccaa accgctggca aaacctgcca gccgctcagg caatggccaa tgccgatgat
    31261 ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc
    31321 tgtcaggacg atccaattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg
    31381 gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg aagccgatct ggagtatgtc
    31441 attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc
    31501 accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa
    31561 ggtttgaatg ccatctggcc acgcattatt gccctggtgg ttcaacccgg cgtcgaattc
    31621 gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc
    31681 gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatct
    31741 ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc gctgaccttc
    31801 gccctgcgtg aagccctttt ttctctggcg gcgattgaag aagaactggt accagcgaaa
    31861 gcctgttccg gtctgcgtca ggtgctggaa aacgtgatgc tcgaccgccc ggaatactgg
    31921 caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta cagctactca
    31981 gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt
    32041 cgtaatctgg cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcag
    32101 tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tataaaccat
    32161 attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc aaaacaaaga
    32221 ggaacatgct atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca
    32281 ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga
    32341 ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc
    32401 cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca
    32461 gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt
    32521 tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc
    32581 caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg
    32641 ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtggacctct ttaaattact
    32701 ttgagggatt catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct
    32761 ttatcgctgg cctagatatg tttaacggct taacccatat gcaccgcccg gtggtcctcg
    32821 gcccgttggt cgggctggta cttggcgatc tgcataccgg aattttaacc ggcgatacgc
    32881 tggaactagt gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta
    32941 tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg
    33001 tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg
    33061 tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca
    33121 ttgaacgcgt gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt
    33181 tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac
    33241 gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc
    33301 tgctgaaaat catgatgaaa aacgtctaca taccctactt catcctgggc tttgttgccg
    33361 caacctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg atggcgctga
    33421 tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg
    33481 aagatggcat ctaatcaaac caccctgccg aacgtcagcg aaaatgaaga aacactgctg
    33541 actggcgtca atgaaaacgt gtatgaagat cggagcattg ccgcggagct gacgaaaaaa
    33601 gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt
    33661 atgcaggctt ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat
    33721 aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt
    33781 ctggtgacgt ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac
    33841 agtattcaga gcaccaaaat tgccgtcggt gcgccgcttg gcgggattgg cgatgcgatg
    33901 ttctggctaa cgctactgcc gatttgtggc gggataggcg caagcctcgc gctacaaggc
    33961 tctattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtacactt ggggctgcgt
    34021 tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattcccct tattaaagcg
    34081 aataccaaaa aagtcggcca tgctgcgtca atcgttggaa tgacggtaat cggcgcactg
    34141 gtggcaacct atgttcgttt aagcaccacg ctggaaatca cggcgggcga cgcagtagtt
    34201 aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgct ggtctacacc
    34261 ctgaccatgt tctggctggt acgccgcggc tagagtccgc tgcgcctgat tgcggttacc
    34321 gtggttctag ggatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga
    34381 tgttaagtat tattttgaca gggcatggcg ggtttgccag tggcatggaa aaagcaatga
    34441 agcaaatcct cggcgatcaa tcgcagttta ttgccatcga ttttcccgaa acctcaagcg
    34501 ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca
    34561 ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcga
    34621 tgcaaaaacc gggctgtgaa gtgattaccg gcaccaattt gcaactgcta ctggagatgg
    34681 tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac
    34741 accgagggct gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg
    34801 aggaaggaat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggtt
    34861 cgatgaccat cagttgcgtg ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt
    34921 gggcgtgact gaacgcgatg cggaacggct ctgccccgct tacatcgaca cccatgtaca
    34981 cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat
    35041 gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc accgtaaccg cgccgcttaa
    35101 taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc
    35161 gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca
    35221 tccg
//
LOCUS       DAJTMX010000027        34938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTMX010000027 DAJTMX010000000
VERSION     DAJTMX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.027"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..783)
                     /locus_tag="OO145_001356"
     CDS             complement(<1..783)
                     /locus_tag="OO145_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5466525.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OO145_001357"
                     /pseudo
     CDS             875..961
                     /locus_tag="OO145_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1366..1842)
                     /locus_tag="OO145_001358"
     CDS             complement(1366..1842)
                     /locus_tag="OO145_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5466526.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO145_001359"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO145_001359"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5466527.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="OO145_001360"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="OO145_001360"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5466528.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..5468
                     /gene="bioF"
                     /locus_tag="OO145_001361"
     CDS             4314..5468
                     /gene="bioF"
                     /locus_tag="OO145_001361"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5466529.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5455..6210
                     /gene="bioC"
                     /locus_tag="OO145_001362"
     CDS             5455..6210
                     /gene="bioC"
                     /locus_tag="OO145_001362"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5466530.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6203..6880
                     /gene="bioD"
                     /locus_tag="OO145_001363"
     CDS             6203..6880
                     /gene="bioD"
                     /locus_tag="OO145_001363"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5466531.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7459..9480
                     /gene="uvrB"
                     /locus_tag="OO145_001364"
     CDS             7459..9480
                     /gene="uvrB"
                     /locus_tag="OO145_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5466532.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO145_001365"
     CDS             complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO145_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5466533.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10845..10989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10977..11966
                     /gene="moaA"
                     /locus_tag="OO145_001366"
     CDS             10977..11966
                     /gene="moaA"
                     /locus_tag="OO145_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5466534.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11988..12500
                     /gene="moaB"
                     /locus_tag="OO145_001367"
     CDS             11988..12500
                     /gene="moaB"
                     /locus_tag="OO145_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5466535.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12503..12988
                     /gene="moaC"
                     /locus_tag="OO145_001368"
     CDS             12503..12988
                     /gene="moaC"
                     /locus_tag="OO145_001368"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5466536.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12981..13226
                     /gene="moaD"
                     /locus_tag="OO145_001369"
     CDS             12981..13226
                     /gene="moaD"
                     /locus_tag="OO145_001369"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5466537.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13228..13680
                     /gene="moaE"
                     /locus_tag="OO145_001370"
     CDS             13228..13680
                     /gene="moaE"
                     /locus_tag="OO145_001370"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5466538.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13817..14521
                     /locus_tag="OO145_001371"
     CDS             13817..14521
                     /locus_tag="OO145_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5466539.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14726..15439
                     /locus_tag="OO145_001372"
     CDS             14726..15439
                     /locus_tag="OO145_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5466540.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15475..16431)
                     /locus_tag="OO145_001373"
     CDS             complement(15475..16431)
                     /locus_tag="OO145_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5466541.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO145_001374"
     CDS             complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO145_001374"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5466542.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17669..18430)
                     /locus_tag="OO145_001375"
     CDS             complement(17669..18430)
                     /locus_tag="OO145_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5466543.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHISDHAPLSAEIHL"
     gene            18563..18973
                     /locus_tag="OO145_001376"
     CDS             18563..18973
                     /locus_tag="OO145_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5466544.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18935..20041)
                     /locus_tag="OO145_001377"
     CDS             complement(18935..20041)
                     /locus_tag="OO145_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5466545.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20052..21185)
                     /locus_tag="OO145_001378"
     CDS             complement(20052..21185)
                     /locus_tag="OO145_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5466546.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21178..22914)
                     /locus_tag="OO145_001379"
     CDS             complement(21178..22914)
                     /locus_tag="OO145_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5466547.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO145_001380"
     CDS             complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO145_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5466548.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO145_001381"
     CDS             complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO145_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5466549.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24805..25992
                     /gene="rhlE"
                     /locus_tag="OO145_001382"
                     /pseudo
     CDS             24805..25992
                     /gene="rhlE"
                     /locus_tag="OO145_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO145_001383"
     CDS             complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO145_001383"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5466550.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26801..28951
                     /gene="dinG"
                     /locus_tag="OO145_001384"
     CDS             26801..28951
                     /gene="dinG"
                     /locus_tag="OO145_001384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5466551.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28979..29941
                     /gene="ybiB"
                     /locus_tag="OO145_001385"
     CDS             28979..29941
                     /gene="ybiB"
                     /locus_tag="OO145_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5466552.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30082..31141
                     /gene="hcxB"
                     /locus_tag="OO145_001386"
                     /pseudo
     CDS             30082..31141
                     /gene="hcxB"
                     /locus_tag="OO145_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
     gene            complement(31370..31630)
                     /locus_tag="OO145_001387"
     CDS             complement(31370..31630)
                     /locus_tag="OO145_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5466553.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31895..32161)
                     /gene="ybiI"
                     /locus_tag="OO145_001388"
     CDS             complement(31895..32161)
                     /gene="ybiI"
                     /locus_tag="OO145_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5466554.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32235..32912)
                     /gene="ybiX"
                     /locus_tag="OO145_001389"
     CDS             complement(32235..32912)
                     /gene="ybiX"
                     /locus_tag="OO145_001389"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5466555.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32954..34938)
                     /locus_tag="OO145_001390"
                     /pseudo
     CDS             complement(32954..34938)
                     /locus_tag="OO145_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8282 a         8957 c         9434 g         8265 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa
     4741 gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg
     4801 cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg
     4861 atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg
     4921 tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc
     4981 aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg
     5041 ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta
     5101 tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca
     5161 ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc
     5221 gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat
     5281 tgatcgtcgg tgataatagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct
     5341 gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa
     5401 cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca
     5461 acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa
     5521 catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa
     5581 tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa
     5641 cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat
     5701 aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg
     5761 acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca
     5821 ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg
     5881 cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct
     5941 aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat
     6001 catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa
     6061 ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag
     6121 ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat
     6181 ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga
     6241 agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg
     6301 gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa
     6361 tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa
     6421 tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc
     6481 gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt
     6541 gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga
     6601 ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat
     6661 taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg
     6721 ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac
     6781 ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa
     6841 tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa
     6901 ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat
     6961 cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg
     7021 ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta
     7081 gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc
     7141 tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca
     7201 ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc
     7261 tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg
     7321 attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc
     7381 agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac
     7441 tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct
     7501 ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac
     7561 cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct
     7621 gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat
     7681 ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac
     7741 tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg
     7801 gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt
     7861 gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat
     7921 ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc
     7981 cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc
     8041 gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg
     8101 gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt
     8161 gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag
     8221 cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg
     8281 ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg
     8341 gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc
     8401 ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg
     8461 ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac
     8521 cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac
     8581 cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg
     8641 acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt
     8701 ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat
     8761 cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg
     8821 accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt
     8881 tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg
     8941 ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa
     9001 gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg
     9061 ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc
     9121 gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa
     9181 cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc
     9241 gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg
     9301 cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag
     9361 aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa
     9421 gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa
     9481 caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag
     9541 gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc
     9601 acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct
     9661 tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg
     9721 cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct
     9781 ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca
     9841 acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc
     9901 aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg
     9961 gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata
    10021 atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc
    10081 gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact
    10141 tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa
    10201 tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta
    10261 aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg
    10321 ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg
    10381 aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc
    10441 gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt
    10501 gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca
    10561 gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc
    10621 aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta
    10681 tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca
    10741 ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatacatg attacatagc
    10801 gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact
    10861 ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga
    10921 ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg
    10981 cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg
    11041 atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca
    11101 ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc
    11161 tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg
    11221 atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg
    11281 gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg
    11341 tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca
    11401 accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca
    11461 ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc
    11521 agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc
    11581 tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct
    11641 ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt
    11701 acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc
    11761 gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta
    11821 acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag
    11881 cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc
    11941 aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa
    12001 gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag
    12061 aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg
    12121 tcgatgaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga
    12181 tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg
    12241 atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag
    12301 tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag
    12361 cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta
    12421 ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc
    12481 atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc
    12541 gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt
    12601 tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga
    12661 cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat
    12721 cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga
    12781 gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat
    12841 ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt
    12901 gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg
    12961 tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg
    13021 tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc
    13081 acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg
    13141 ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt
    13201 tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag
    13261 ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta
    13321 gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc
    13381 ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt
    13441 aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc
    13501 gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg
    13561 cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg
    13621 gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag
    13681 ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg
    13741 tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata
    13801 tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc
    13861 tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac
    13921 cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa
    13981 ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc
    14041 gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct
    14101 gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac
    14161 tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg
    14221 cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc
    14281 gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt
    14341 gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca
    14401 gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt
    14461 gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta
    14521 attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga
    14581 cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat
    14641 ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
    14701 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
    14761 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
    14821 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
    14881 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
    14941 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
    15001 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
    15061 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    15121 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
    15181 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
    15241 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
    15301 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
    15361 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    15421 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    15481 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
    15541 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    15601 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    15661 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    15721 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    15781 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    15841 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    15901 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    15961 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    16021 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
    16081 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    16141 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    16201 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    16261 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    16321 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    16381 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
    16441 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
    16501 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    16561 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
    16621 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    16681 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
    16741 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    16801 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
    16861 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    16921 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    16981 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
    17041 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    17101 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    17161 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    17221 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    17281 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    17341 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
    17401 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    17461 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    17521 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    17581 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    17641 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    17701 gatcagaaat gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    17761 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    17821 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    17881 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    17941 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    18001 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
    18061 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    18121 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    18181 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    18241 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    18301 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    18361 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    18421 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    18481 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    18541 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    18601 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    18661 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    18721 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    18781 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    18841 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
    18901 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    18961 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    19021 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    19081 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
    19141 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    19201 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    19261 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
    19321 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    19381 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    19441 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    19501 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
    19561 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
    19621 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    19681 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    19741 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
    19801 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
    19861 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    19921 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
    19981 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    20041 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    20101 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    20161 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    20221 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
    20281 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    20341 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    20401 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
    20461 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
    20521 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    20581 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
    20641 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
    20701 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
    20761 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    20821 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    20881 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
    20941 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    21001 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    21061 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    21121 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
    21181 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    21241 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
    21301 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    21361 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    21421 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
    21481 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
    21541 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
    21601 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    21661 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
    21721 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
    21781 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
    21841 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    21901 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    21961 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
    22021 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
    22081 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    22141 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    22201 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    22261 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    22321 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    22381 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    22441 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
    22501 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    22561 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    22621 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    22681 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
    22741 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
    22801 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    22861 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    22921 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
    22981 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    23041 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
    23101 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    23161 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    23221 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
    23281 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    23341 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    23401 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    23461 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    23521 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
    23581 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    23641 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    23701 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    23761 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    23821 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
    23881 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    23941 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    24001 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    24061 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
    24121 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
    24181 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    24241 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
    24301 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
    24361 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    24421 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    24481 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    24541 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    24601 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
    24661 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    24721 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    24781 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    24841 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    24901 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    24961 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    25021 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    25081 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    25141 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    25201 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    25261 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    25321 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    25381 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    25441 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    25501 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    25561 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    25621 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    25681 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    25741 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    25801 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    25861 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    25921 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    25981 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    26041 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    26101 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    26161 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    26221 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    26281 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    26341 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    26401 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    26461 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    26521 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    26581 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    26641 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    26701 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    26761 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    26821 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    26881 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    26941 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    27001 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    27061 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    27121 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    27181 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    27241 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    27301 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    27361 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    27421 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    27481 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    27541 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    27601 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    27661 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    27721 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    27781 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    27841 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    27901 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    27961 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    28021 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    28081 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    28141 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    28201 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    28261 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    28321 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    28381 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    28441 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    28501 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    28561 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    28621 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    28681 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    28741 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    28801 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    28861 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    28921 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    28981 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    29041 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    29101 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    29161 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    29221 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    29281 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    29341 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    29401 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    29461 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    29521 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    29581 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    29641 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    29701 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    29761 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    29821 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    29881 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    29941 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    30001 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    30061 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    30121 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    30181 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    30241 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    30301 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    30361 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    30421 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    30481 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    30541 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    30601 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    30661 gccaggttgc ctgattgacg ttaacggcgt gccgacgacc aatccggcgg taatgcagga
    30721 gtcgccgttg gggtcgctgt tgacctttgc cgaacataaa ggctacgccc ttgcagcgat
    30781 gtgtgaaatt cttggcgggg cgctttctgg cggtaaaacg acgcatcagg aaacgttgca
    30841 aaccagcccc gatgccattc ttaactgcat gaccactatc atcatcaacc cggaactgtt
    30901 cggcgcgccg gattgtaacg cgcagaccga agcctttgcc gagtgggtga aagcctcgcc
    30961 gcatgatgac gataagccga ttttgctacc gggcgagtgg gaagtgaaca cgcgtcgcga
    31021 acggcaggag caggggattc cattggatgc gggaagctgg caggccattt gtgatgcggc
    31081 gcggcagatt ggtatgtcgg aagagacgtt gcaggctttc tgtcagcagt tagccagcta
    31141 aaaaaaagcc cgtccagtgg cggacgggca aacaagggta acataggatc aatgagggtt
    31201 agagcatatg cgtctgtcgg caaacagaca gggaaatact tgtgctggac gtagcgtaaa
    31261 cgcctgatcc gtccaacggt tcggattttg taggcctgat aagacgcggt agcgtcgcat
    31321 caggcatctg ttgccggatg cggcataaac gccttatccg tcctacgaat tacttataaa
    31381 ttaccgcagt accgcttaat ttattgttgt tggtggcgga agtgatgctg tatccactgg
    31441 caccggctgc cgccgctttc tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc
    31501 catcggcaga aaccacgccg attttattca tgttctgtgc ctgggatgcc gttaccggtt
    31561 ccgccgcgaa cacgccaaat gacagagttg aaagagccat agcagcaaca acagtattga
    31621 tagttttcat aattaatctc tcgcaggtga tttttgtata agtgacgttg tttcgtcgat
    31681 gtgatgagta tcacgttttt ttacgagaga taaaatcgaa gagaattgac cgccttgttc
    31741 aaataaattg attgatatct aacatactga taaaccattt atttgttgta cttatggact
    31801 tgttttatgc cgcgtggcag tcacagcagc gttaaaggca cggaatgcca cgcaaaatac
    31861 aaattatact cgctatgcat ctcgccagtt gtagttaacg taactgtctg tctttcgaac
    31921 ctctgcgatt atatcctgta tacgctggtt tttgtaaatc tttctcctgc tgacaatgaa
    31981 tacataagcg cacgccagga atggcttccc gacgggcttg cgggatgggg gcaccgcact
    32041 cttcacattc atacaggctt tcgccacgcg gaatttcacc ccgagcgcgg gcaatcgcat
    32101 cttcaattgt actgttgatc tgttcgttga cggcgtcatc gttagcccaa gcggatgcca
    32161 tatcgtcctc cccatatcaa tgcttgtaca gttaagtgta gctaatatag ggacgaacct
    32221 gggcagttca agcgtcagat ctccgaccat tcccgcagca gattatgata aagattgagc
    32281 agcgacagga tctcttcatt ttcaccatag cggcttttca gcgactgaat atttttgtcc
    32341 agttcaaaca gcatggcgcg ctttttatca tcgcggatca tcgactggat ccacataaat
    32401 gatgccactc gtacgccgcg ggttacgggt gtcacgcaat gcaggctgct ggagggatac
    32461 aacaccagat cacctgccgg gagttttacc cgatgttgtc caaaggtgtc attaacgacc
    32521 agttcgccgc cgtcgtagct ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagtg
    32581 cgcatccagc cgttttgtgg atggctgcgt actgcgccat ccacatggaa accataggtt
    32641 tcattgttct gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa ggcagcggca
    32701 aagaacaatg catgttggtt aaccgcgttc agcacctcat tttgcagtgc ggcgtataac
    32761 gcgctgcggg tgtctacttg ctgattgttc ttaacttgtg caccttgtgc tccggtggtg
    32821 acgcgtccat ccacccattc ggcttgttcc agttgttcgc gaaaatgggc gacgtcctgt
    32881 ggcgataaca cgccgggaat gtggtacatc atacaagttc tccaaaaagt ggggcctgcg
    32941 ccccacatct gaatcagaaa tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg
    33001 ggtgataacg gtagccgctc ttgttgattg aggcgacgta atcggtatca aacaggttgt
    33061 aaacgtttag ctggaagtcg agattgcgat taacccgata ccccagtttg gcatcggcga
    33121 cccagtaacc ttctgtaaat gctggcgttc ccaccgcgcc gtctgaacct ttatgtatac
    33181 tgccgatata gcgtgcgccc gcgccaacag agatatcatc ggttgcctga tattggctcc
    33241 ataaggtgaa ggcgtgctcc ggggtatacg gcagcgatga ggaaccatcc tgggcaacat
    33301 ctttgccgtt tttgatggtt gctttttgct gggtatagcc gccaatcacc tgccacgcgg
    33361 gagtgatatt ccctgccacg gatatctcat agccttcgac gcgtttctta ccgtattgcg
    33421 agtaagtccc gtcatcattt tgctcaactt cattttcgat atcagtacgg aacagcgcgg
    33481 cggtgagcaa caggcgttta tccagaacct gccatttggt gccaatctcg ctggtgttgg
    33541 ctttttgcgg tttaaaatcg gtgcggttgg cactgttacc actgccagac tgcgcaaggg
    33601 cgaagttgtt gccgcccgga ggctgctggg aaacggcata gttaatatag acattgccgt
    33661 tttccgtcag gtggtacagc gccccggctt tccagttcac cagattgccc gacttggcgg
    33721 tgtcgacggt ggtgactgga gaaccttttg ccacaccagc tgggcaggtg atggcaccgc
    33781 gtccgctgcc gccgcaggcg gtggcactgt catattcagt atgataatta tccagacgga
    33841 tcccgccgtt cagctcaaaa tcacgggtga tttgcagcgt atcgaaggcg taaattgcga
    33901 aggtatccgt ctgaccattg gcgtttgcgc cgttgcgcgt caggccgccg ggatgaattc
    33961 tgctgtcagg atgataaata tttaccgccg gtaacgtcac cggattaacg ccatagttag
    34021 tctgcgtttc acgggtaaat tccacgccgg tactgacatc atgaccgata gaaccggtat
    34081 agaacgtcga ggtcaggttg gtctggttgg tgagaatttt attactcaca tctttggtat
    34141 tcgccgtgcg tgactaggtc cagctattca catcgctggt gggctgagta atattcgacg
    34201 ccccgcccat aatcgccgtc atcaggtaat cctgctttac gcgcgaccaa cgggtagtat
    34261 tgcgaatggt ggtgttatcg ttgatgtcgt gctcaaaacg catggtggcg gtgtcggtgg
    34321 tcgaatcgtc gtaatcggaa tccgtgccgt aaaagttatg agtatcaact tttccggaat
    34381 gattcagggc cgccgctccc gcagatgggg cagaatagcc cggcaaaccg atggtcggaa
    34441 tgccgccgtc tggcgtgttg tgctgagtga catgcagata attaagatac aaacgatttg
    34501 ctgtaccaag gccaaaagcg acagaagggg cgacgccgta acgctcattt ttgactttgt
    34561 cgcgtccggc atcgtgggtt ttttcgccca tcacattcag gcgcaccgcg gtagtatcac
    34621 caatgacctg attgacgtcc agcgtgccgc ggcggaacca ggcgctgcca atactggcgg
    34681 aggcgtcaat gccggaatca ttgcgcggct gcttgctgat catattgatc gagcctgtcg
    34741 gtgcgctgcg cccgtagtcg gtgccggacg gccctttaat cacttcgacc tgctcggtat
    34801 tgaaggtgtc gcgcgagacg ctgccgatat cgcgaatacc atcaatataa atactgttag
    34861 aggtatcggc accacgcata taaatggcgt cgccagtggt ggagttaccg ttctcacccg
    34921 caaaaaacgc acccacgc
//
LOCUS       DAJTMX010000028        34788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTMX010000028 DAJTMX010000000
VERSION     DAJTMX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.028"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..2098
                     /locus_tag="OO145_001391"
     CDS             <1..2098
                     /locus_tag="OO145_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5466556.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO145_001392"
     CDS             2095..3183
                     /locus_tag="OO145_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5466557.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO145_001393"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO145_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5466558.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO145_001394"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO145_001394"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5466559.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO145_001395"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO145_001395"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5466560.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO145_001396"
     CDS             8051..8602
                     /locus_tag="OO145_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5466561.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO145_001397"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO145_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5466562.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO145_001398"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO145_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5466563.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRDHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO145_001399"
     CDS             complement(11683..12681)
                     /locus_tag="OO145_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5466564.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO145_001400"
     CDS             complement(12760..13452)
                     /locus_tag="OO145_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5466565.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO145_001401"
     CDS             complement(13529..14032)
                     /locus_tag="OO145_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5466566.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO145_001402"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO145_001402"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5466567.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO145_001403"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO145_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5466568.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO145_001404"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO145_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5466569.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO145_001405"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO145_001405"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5466570.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO145_001406"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO145_001406"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5466571.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO145_001407"
     CDS             complement(20922..21341)
                     /locus_tag="OO145_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466572.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO145_001408"
     CDS             complement(21319..21672)
                     /locus_tag="OO145_001408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466573.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO145_001409"
     CDS             complement(21673..21993)
                     /locus_tag="OO145_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466574.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO145_001410"
     CDS             complement(22127..23560)
                     /locus_tag="OO145_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5466575.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO145_001411"
     CDS             complement(23623..24072)
                     /locus_tag="OO145_001411"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5466576.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO145_001412"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO145_001412"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5466577.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO145_001413"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO145_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5466578.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO145_001414"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO145_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO145_001415"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO145_001415"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5466579.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO145_001416"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO145_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO145_001417"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO145_001417"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5466580.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO145_001418"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO145_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO145_001419"
     tRNA            complement(34082..34157)
                     /locus_tag="OO145_001419"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO145_001420"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO145_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8483 a         8944 c         9142 g         8219 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag tgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgatcgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DAJTMX010000029        34444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTMX010000029 DAJTMX010000000
VERSION     DAJTMX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.029"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..331)
                     /locus_tag="OO145_001421"
     CDS             complement(<1..331)
                     /locus_tag="OO145_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466581.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="OO145_001422"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="OO145_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5466582.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="OO145_001423"
     CDS             complement(1692..2072)
                     /locus_tag="OO145_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5466583.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="OO145_001424"
     CDS             complement(2086..2502)
                     /locus_tag="OO145_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466584.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="OO145_001425"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="OO145_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5466585.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="OO145_001426"
     CDS             complement(3503..3970)
                     /locus_tag="OO145_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5466586.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="OO145_001427"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="OO145_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5466587.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="OO145_001428"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="OO145_001428"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5466588.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="OO145_001429"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="OO145_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5466589.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="OO145_001430"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="OO145_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5466590.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="OO145_001431"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="OO145_001431"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5466591.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="OO145_001432"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="OO145_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5466592.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="OO145_001433"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="OO145_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5466593.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="OO145_001434"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="OO145_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5466594.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="OO145_001435"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="OO145_001435"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5466595.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="OO145_001436"
     CDS             complement(14625..15440)
                     /locus_tag="OO145_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5466596.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQALADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="OO145_001437"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="OO145_001437"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5466597.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="OO145_001438"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="OO145_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5466598.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="OO145_001439"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="OO145_001439"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5466599.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="OO145_001440"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="OO145_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5466600.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="OO145_001441"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="OO145_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5466601.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="OO145_001442"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="OO145_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5466602.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="OO145_001443"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="OO145_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5466603.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="OO145_001444"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="OO145_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5466604.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="OO145_001445"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="OO145_001445"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5466605.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="OO145_001446"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="OO145_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5466606.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="OO145_001447"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="OO145_001447"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5466607.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="OO145_001448"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="OO145_001448"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5466608.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="OO145_001449"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="OO145_001449"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5466609.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="OO145_001450"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="OO145_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5466610.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="OO145_001451"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="OO145_001451"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5466611.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="OO145_001452"
     CDS             31574..32188
                     /locus_tag="OO145_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5466612.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="OO145_001453"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="OO145_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5466613.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="OO145_001454"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="OO145_001454"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5466614.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8107 a         8699 c         9303 g         8335 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg tcacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 catcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 tttcctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ttggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cgcctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc cctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgtaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgataattt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgtatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtaat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgcagc cgggtcagcg gggttctcct gagaattccg
    34381 acagagaaag caaaaataaa tgcttgactt tgtagcggga aagcgtatta tgaacacccc
    34441 gcgc
//
LOCUS       DAJTMX010000030        33544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTMX010000030 DAJTMX010000000
VERSION     DAJTMX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.030"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            529..666
                     /locus_tag="OO145_001455"
     CDS             529..666
                     /locus_tag="OO145_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466615.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO145_001456"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO145_001456"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5466616.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /gene="virB"
                     /locus_tag="OO145_001457"
     CDS             complement(2992..3921)
                     /gene="virB"
                     /locus_tag="OO145_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5466617.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="OO145_001458"
     CDS             complement(4138..4374)
                     /locus_tag="OO145_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5466618.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="OO145_001459"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="OO145_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5466619.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="OO145_001460"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="OO145_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5466620.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="OO145_001461"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="OO145_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5466621.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="OO145_001462"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="OO145_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5466622.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="OO145_001463"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="OO145_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5466623.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="OO145_001464"
     CDS             complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="OO145_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5466624.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="OO145_001465"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="OO145_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5466625.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="OO145_001466"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="OO145_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5466626.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="OO145_001467"
     CDS             13562..15178
                     /locus_tag="OO145_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5466627.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="OO145_001468"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="OO145_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5466628.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="OO145_001469"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="OO145_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5466629.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="OO145_001470"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="OO145_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5466630.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /gene="mxiH"
                     /locus_tag="OO145_001471"
     CDS             17140..17391
                     /gene="mxiH"
                     /locus_tag="OO145_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5466631.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="OO145_001472"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="OO145_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5466632.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /gene="mxiJ"
                     /locus_tag="OO145_001473"
     CDS             17703..18428
                     /gene="mxiJ"
                     /locus_tag="OO145_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5466633.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="OO145_001474"
     CDS             18425..18952
                     /locus_tag="OO145_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5466634.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /gene="mxiN"
                     /locus_tag="OO145_001475"
     CDS             18894..19589
                     /gene="mxiN"
                     /locus_tag="OO145_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5466635.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /gene="mxiM"
                     /locus_tag="OO145_001476"
     CDS             19582..19989
                     /gene="mxiM"
                     /locus_tag="OO145_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5466636.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="OO145_001477"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="OO145_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5466637.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="OO145_001478"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="OO145_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5466638.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="OO145_001479"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="OO145_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5466639.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="OO145_001480"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="OO145_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5466640.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="OO145_001481"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="OO145_001481"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5466641.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spa15"
                     /locus_tag="OO145_001482"
                     /gene_synonym="spaK"
     CDS             26022..26423
                     /gene="spa15"
                     /locus_tag="OO145_001482"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5466642.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26427..27719
                     /gene="spaL"
                     /locus_tag="OO145_001483"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26427..27719
                     /gene="spaL"
                     /locus_tag="OO145_001483"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5466643.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27812..28150
                     /gene="spa13"
                     /locus_tag="OO145_001484"
                     /gene_synonym="spaM"
     CDS             27812..28150
                     /gene="spa13"
                     /locus_tag="OO145_001484"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5466644.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="OO145_001485"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="OO145_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5466645.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spa33"
                     /locus_tag="OO145_001486"
                     /gene_synonym="spaO"
     CDS             29009..29890
                     /gene="spa33"
                     /locus_tag="OO145_001486"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5466646.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spa24"
                     /locus_tag="OO145_001487"
                     /gene_synonym="spaP"
     CDS             29895..30545
                     /gene="spa24"
                     /locus_tag="OO145_001487"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5466647.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="OO145_001488"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="OO145_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5466648.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spa29"
                     /locus_tag="OO145_001489"
                     /gene_synonym="spaR"
     CDS             30820..31590
                     /gene="spa29"
                     /locus_tag="OO145_001489"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5466649.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHEFFTNLFVR"
     gene            31597..32625
                     /gene="spa40"
                     /locus_tag="OO145_001490"
                     /gene_synonym="spaS"
     CDS             31597..32625
                     /gene="spa40"
                     /locus_tag="OO145_001490"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5466650.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="OO145_001491"
     CDS             32638..32880
                     /locus_tag="OO145_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466651.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10466 a         4873 c         6718 g        11487 t
ORIGIN      
        1 ttgttgacta cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggtgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg accaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atatgatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtgga ttttttgttt atcaccatgt
     2641 aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatattc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgtagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttcttatat ttgcatctgt aacttttaat ttgttgttta ttctatgttc
     4681 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaagcctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgccat ttccacctag attatctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatactga caacatatcc cccgtttttt tgagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagttcttcc aatgcctttt taagcgagtt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat
     6901 atcatttgat ctccatcaag ctcggcttct ttcggagctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatattc gtgcctggaa agaatatcta atagttgagc ggatttattt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7141 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7381 ttgattggtt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtttcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7621 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7741 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8161 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct
     8401 gggttggttt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8641 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattttctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9661 tttttctttt ctggtgactc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtgt taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgctgg gacttccaag cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10021 tgtgcattag ttgtattaat attttggtta gcagtaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcttgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggaaa accagtggtt gtggtgctta cattatgcat aataattact
    10201 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttagcttt taagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aatactggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaacctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcggtga tatttaaaga cataaggtct cctttttgtc
    10681 tgctttactt taataagtat aagatttaat ataaaagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tatagcgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataagt
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgcgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcat acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgatctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caataatttt
    11881 tattgatttt tcgatgattt cttctttttt atctgttgct tcggttttta attcgtttaa
    11941 tacacgagtc atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttggtg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatttc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 atcgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt ctttttgcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgc ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact agtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaaaacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 acgctctgcg gtattcaaca ttaatttgat gtctctacct gtgaaagagt gttgaattga
    12961 gtttttagct gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 attgaggaaa agcaataagg atttttgatt ctctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta tcaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg aggatcatac tttattaact
    13261 ctccattact tggtgattgt ggggatgtaa aatgtatttg gtatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctcgttt catcatgaaa tcccacaaga taaagtgcct
    13441 gatgtatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagaa agaaataagg ggatcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga gctgaagttt tttgcgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac gattagatac
    13861 ctataaactt ggtgaagtta ataaaagaca tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaaacga aaataaaaca
    13981 aatatttact gattatttaa ataataagaa ttggggaccg gtcgacaaga atattagtca
    14041 tcatgggaaa aattatggtt ttcaattaac tcctgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 tgatcatatc gcgaatatgt ggctttcaaa agtggtggat gatgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
    14341 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
    14461 cgcattaaaa gggttaaact ctaaaagggg gaagccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attgtaacca aaaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
    15121 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
    15241 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgctta gctactgatg atgaaggcgg
    15421 aaatctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggatg tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt ttatcccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaatattc catcaagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tctgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tattctgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgatgat gtatggaaga gggtgggagg ctgttggagc ctataatgca
    15901 ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctatagacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gacaatttcc cagaaaatat tattcctgta acagatagta
    16201 aatcggatgg tataatttat ttgaccataa gcaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga gcaaattgat atcaattcac aatttaattc atttgaatat gacgggattt
    16321 cttttcacct taaaaatatg agggaggata aatcgagagg ccatatacta aacggaatgt
    16381 ataagaacca ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacactgga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcgtc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 caaacaaaga aataaataga attcaacaat atatcaatca acgattccca tttatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtgttttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgata aattgaaaaa tgctctgatt gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg ggggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaaat aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgaacataa aaatatatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccgaat
    17161 gatgattgga cattgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 ggtgaactaa cattggcact agataaatta gctaaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atccaataca
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagtct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaagt taaatcggaa tttttaacaa atgaagtaat atatttattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttggtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg tgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaatagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaagt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgcaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgcttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttcagctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaactg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagcccaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatatagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggtgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc gtcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaataat atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataaaagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaacgttaa
    23161 aagataacct tcctctagat cgggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatt attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gacgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga agtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agataaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtccctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataaatgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta attttgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tggggtctta taatcaatga gctatacaaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tgttgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acagataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtattaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttactga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttagaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagataa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggatc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcagtc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgactcattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgcactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagaaca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 tcttttagaa aaaaatatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgacatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtacct gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc tcaaaaattt gatacaatat ctgatattgt ccgtttttct gattctggct
    30181 tgatggaata taagcaatac ttgaaaaagc atacagatct tgagttagca cgttttttcc
    30241 aaaggtctga agaagaaaat gctgatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaatcaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttgtt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtggggtatc ctgtctaagg ccttgattga acaatatatc aatattcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat tatctattgg
    30661 tttgttacag acagtgactc aactccaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt ggctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggcctcct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggtat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tcttaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggtgaacat
    31561 gaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgactgttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg gaggctgttt aaggatataa tattattttt cttggcattt
    32161 tctattcttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttattttaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgcactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacggagac aggtaaatta tggacgaaaa gaagcttaaa gcccttgcgg
    33421 ctgaactggc taaaggcctc aaaactgagg ccgacctcaa tcagttttcc cgtatgctga
    33481 cgaaatatcc cggctcttaa aacgatagtg gctcaggctt tcatttttca gcgttcccca
    33541 gaag
//
LOCUS       DAJTMX010000031        33057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTMX010000031 DAJTMX010000000
VERSION     DAJTMX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.031"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OO145_001492"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OO145_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5466652.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OO145_001493"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OO145_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5466653.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OO145_001494"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OO145_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5466654.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OO145_001495"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OO145_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5466655.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OO145_001496"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OO145_001496"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5466656.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OO145_001497"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OO145_001497"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5466657.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OO145_001498"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OO145_001498"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5466658.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="OO145_001499"
     CDS             complement(4523..5245)
                     /locus_tag="OO145_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5466659.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OO145_001500"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OO145_001500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5466660.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="OO145_001501"
     CDS             6445..6663
                     /locus_tag="OO145_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5466661.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OO145_001502"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OO145_001502"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5466662.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="OO145_001503"
     CDS             complement(7397..7597)
                     /locus_tag="OO145_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5466663.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7607..9344)
                     /gene="kefB"
                     /locus_tag="OO145_001504"
                     /pseudo
     CDS             complement(7607..9344)
                     /gene="kefB"
                     /locus_tag="OO145_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9344..9895)
                     /gene="kefG"
                     /locus_tag="OO145_001505"
     CDS             complement(9344..9895)
                     /gene="kefG"
                     /locus_tag="OO145_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5466664.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10026..11939
                     /locus_tag="OO145_001506"
     CDS             10026..11939
                     /locus_tag="OO145_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5466665.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            11939..12961
                     /locus_tag="OO145_001507"
     CDS             11939..12961
                     /locus_tag="OO145_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5466666.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            12955..13173
                     /locus_tag="OO145_001508"
     CDS             12955..13173
                     /locus_tag="OO145_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5466667.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13227..14096
                     /locus_tag="OO145_001509"
     CDS             13227..14096
                     /locus_tag="OO145_001509"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5466668.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14151..14555)
                     /locus_tag="OO145_001510"
     CDS             complement(14151..14555)
                     /locus_tag="OO145_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5466669.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14857..15489
                     /gene="crp"
                     /locus_tag="OO145_001511"
     CDS             14857..15489
                     /gene="crp"
                     /locus_tag="OO145_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5466670.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15540..17630
                     /locus_tag="OO145_001512"
     CDS             15540..17630
                     /locus_tag="OO145_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5466671.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17697..18917)
                     /gene="argD/dapC"
                     /locus_tag="OO145_001513"
     CDS             complement(17697..18917)
                     /gene="argD/dapC"
                     /locus_tag="OO145_001513"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5466672.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAVFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19003..19566)
                     /gene="pabA"
                     /locus_tag="OO145_001514"
     CDS             complement(19003..19566)
                     /gene="pabA"
                     /locus_tag="OO145_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5466673.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19598..20200)
                     /locus_tag="OO145_001515"
     CDS             complement(19598..20200)
                     /locus_tag="OO145_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5466674.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20190..20357)
                     /locus_tag="OO145_001516"
     CDS             complement(20190..20357)
                     /locus_tag="OO145_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5466675.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20462..21043)
                     /gene="ppiA"
                     /locus_tag="OO145_001517"
                     /gene_synonym="rotA"
     CDS             complement(20462..21043)
                     /gene="ppiA"
                     /locus_tag="OO145_001517"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5466676.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21314..22495
                     /gene="tsgA"
                     /locus_tag="OO145_001518"
     CDS             21314..22495
                     /gene="tsgA"
                     /locus_tag="OO145_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5466677.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22757..25300
                     /gene="nirB"
                     /locus_tag="OO145_001519"
     CDS             22757..25300
                     /gene="nirB"
                     /locus_tag="OO145_001519"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5466678.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25297..25623
                     /gene="nirD"
                     /locus_tag="OO145_001520"
     CDS             25297..25623
                     /gene="nirD"
                     /locus_tag="OO145_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5466679.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25749..26555
                     /gene="nirC"
                     /locus_tag="OO145_001521"
     CDS             25749..26555
                     /gene="nirC"
                     /locus_tag="OO145_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5466680.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26574..27947
                     /gene="cobA"
                     /locus_tag="OO145_001522"
     CDS             26574..27947
                     /gene="cobA"
                     /locus_tag="OO145_001522"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5466681.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28194..28361
                     /locus_tag="OO145_001523"
     CDS             28194..28361
                     /locus_tag="OO145_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5466682.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28656..29993
                     /gene="frlA"
                     /locus_tag="OO145_001524"
                     /pseudo
     CDS             28656..29993
                     /gene="frlA"
                     /locus_tag="OO145_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30014..31036
                     /gene="frlB"
                     /locus_tag="OO145_001525"
     CDS             30014..31036
                     /gene="frlB"
                     /locus_tag="OO145_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5466683.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31086..31916
                     /gene="frlC"
                     /locus_tag="OO145_001526"
     CDS             31086..31916
                     /gene="frlC"
                     /locus_tag="OO145_001526"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5466684.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31913..32698
                     /gene="frlD"
                     /locus_tag="OO145_001527"
     CDS             31913..32698
                     /gene="frlD"
                     /locus_tag="OO145_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5466685.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32798..>33057
                     /locus_tag="OO145_001528"
     CDS             32798..>33057
                     /locus_tag="OO145_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5466686.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTV"
BASE COUNT         7942 a         8834 c         8394 g         7887 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcaggc cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcatt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca cgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcaa aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gctgcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag actcaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcgc ttgccggtga ggtcagttgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaacttatg gccttctgct
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg aaaatcaccg ctcttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcatcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat tcacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacactag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag aaagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc
     5701 agcgtacctt tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct ttaagagagt tttccatgta acgacccagc
     6061 gaagcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgatgc tttgctgtct
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagtag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaagcacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtggcc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ccttttcggt attagtggca
     6721 accacaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc
     6841 gccgtgaacg tggccatgag ccagttcttc ttcggtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca ctacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcagtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcactag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgtgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgctataaaa cgttttcgga ttgccatcct taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc agcatgcatc gggattaact
     7741 cccgcagcat tcgcatatcc aggcggcgaa aatgcagttg cgcccgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccatat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8341 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8401 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8461 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8521 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8581 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8641 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8701 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8761 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaactcc agcaccttca
     8821 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     8881 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     8941 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9001 tcgcaaagcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9061 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9121 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9181 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9241 acgggccaat tgcaatcccc gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9301 aagagaaaca gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag
     9361 acagcggatt tgccaaccag tcaccgtagg ctctggcgtg gctcgccagc tcctttgcgc
     9421 tttgccgtct cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg
     9481 ccgccagttc aaaggggcgc agcacatcgc tcatcgggta gcgattcagc gcgtcataac
     9541 ggtaagcact ttccggctcg ccggtggtaa tcacgttacg ccagtacttt cccgccagtt
     9601 ggtttcctcc cggcccgctg gcaaaaccac gacttaatac ccggtccagc cactctttca
     9661 gtagcgccgg gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc
     9721 gcagtaatgc ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcgtaaaggt
     9781 cgtgcacggt aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg
     9841 agtcctgaga ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc
     9901 ccctcccggt atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg
     9961 gcagctcgcc catacattac attatcataa tgataagtta acatagtctg aacatacggc
    10021 gccttatgat tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata
    10081 atgccaccgc caccatcaac cccgggcaga aagtcggcct ggtgggtaaa aacggctgtg
    10141 gtaaatctac cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca
    10201 cctttccggg aagctggcaa ctggcgtggg tgaatcagga aacgccagcg ttaccgcaag
    10261 cggcgctgga atatgtcatt gacggcgacc gtgaatatcg tcaactggaa gcgcagctgc
    10321 acgacgccaa cgaacgtaac gacgggcacg ccattgcgac cattcatagc aagctggatg
    10381 ctattgacgc atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca
    10441 gcaatgaaca actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta
    10501 accttgccca ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc
    10561 acctcgatct cgatgccgtt atctggctgg aaaaatggtt gaagagctat cagggcacgc
    10621 tgatcctgat ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata
    10681 tcgaacaaca aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg
    10741 ccacccgtct ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtggcacatc
    10801 tgcaaagtta tatcgatcgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc
    10861 gcattaagat gctggaacgt atggagctga ttgcccccgc acacgtcgac aacccgttcc
    10921 gctttagctt ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaggtca
    10981 gcgcaggcta tggcgatcgc attattctcg actcgattaa attgaatctg gtccccggct
    11041 cgcgcattgg tctgttaggc cgcaacggcg caggtaaatc gacattaatc aaactgttag
    11101 ccggtgaact tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct
    11161 acttcgccca gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg
    11221 cacgtttagc gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt
    11281 tcctgggcga taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc
    11341 tggtgctggc attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta
    11401 accaccttga cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg
    11461 cgctggttgt cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc
    11521 tggttcacga tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt
    11581 tgagcgacgt acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca
    11641 gcgcccaggc acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc
    11701 cgctgcgtaa agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg
    11761 cgcaggcgga agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt
    11821 tgaccgcctg cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg
    11881 cgtggctgga agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat
    11941 ggcgcagata acgacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat
    12001 gcgcggcttt agcaattgtc atctacaaac tatgctgccg cgtttgtttc gtcgtcaggt
    12061 gaaattcacc ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc
    12121 gtggagtgaa gaccccgcac aggcgaaaca taaaccgcgt ttagtggtgt ttcacgggct
    12181 ggaaggcagt ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg
    12241 ctggctgggc gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg
    12301 catttaccat tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga
    12361 atttggtcat gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg
    12421 tttgctggca aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc
    12481 gtttatgctg gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg
    12541 ttacttgctg aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac
    12601 gctgccgatt aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct
    12661 aatcaccgcc agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc
    12721 catgccaatg ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc
    12781 gtttatggat catcaggtga tcccaaaacc ggaaagtctc ccctcgcagg tggagtatca
    12841 actgactgaa catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat
    12901 gtggctggag tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg
    12961 attccgtggc aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta
    13021 cgtgaaggca ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc
    13081 aaacgccagc tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc
    13141 aatattatgc cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat
    13201 agtgatagct aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag
    13261 gatccagcgg cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt
    13321 taaatctgca tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa
    13381 tggacatggc gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg
    13441 aagcgaacga cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag
    13501 ggaaatctcg caaatatctg catacctacg acgaagccgt accctggaat caggtaccgg
    13561 gaacattcac cccctggcaa cctttaccag aacccactga tgtactgttt tatgaaggtt
    13621 tacacggcgg cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg
    13681 gcgtagtgcc tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc
    13741 gcgggcactc acgagaagca gtgatggact cggtagtgcg ttcaatggaa gactatatca
    13801 actacatcac accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg
    13861 acacttcaaa cccgttcgcg gcaaaaggta tcccgtcgct ggatgaaagc tttgtggtga
    13921 tccattttcg taatctggaa gggatcgatt tcccttggct gctggcgatg ttgcaaggct
    13981 cattcatttc ccacatcaat acgttagtgg tacctggcgg caaaatgggg ctggcaatgg
    14041 aattaattat gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaacttt
    14101 gctattatgc cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca
    14161 accacttcat acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa
    14221 tatttctcgg cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg
    14281 gtgacgataa aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca
    14341 gaggtcaatt ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc
    14401 acatcgatgg cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct
    14461 ttatcgcctg agttgccgtc cattaaaatc tggtgaccag aggcggattc gcccagaaaa
    14521 gttaaccctt cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt
    14581 tcctgacaga gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg
    14641 aagcgagaca ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt
    14701 aatacattga tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat
    14761 agccccttcc caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg
    14821 gctctggaga aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca
    14881 gacccgactc tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc
    14941 acgcttattc accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg
    15001 gcagtgctga tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt
    15061 gattttattg gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt
    15121 gcgaaaaccg cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag
    15181 gtaaacccgg acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact
    15241 tcagagaaag tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg
    15301 ctgaacctgg caaaacaacc agatgctatg actcacccgg acggtatgca aatcaaaatt
    15361 acccgtcagg aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg
    15421 aagatgctgg aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc
    15481 actcgttaat cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga
    15541 tgtggcgcag actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc
    15601 tatgtttgcc cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct
    15661 ccctcgttcc tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca
    15721 aacacttaat cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac
    15781 tggtaaaaga tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca
    15841 ctgctgagct ggggccatta cacgcaaaat tgcttcctgc atcgctgctc gccgccattt
    15901 ttaccctcag tctggcggga tacatgccgg tctgggaacc attgctcatc tatgcgttgg
    15961 gcactctctg gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc
    16021 tgcgcgagtc actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca
    16081 gcctgcttac ccagcacacc gatcctgaaa aagcgctgcc accgctgctg gtgcgccagc
    16141 aaaaagcggt cgatttaatt actcagtgct atcagcaaat gcatatgctt tccgcgcaaa
    16201 acaataccga ttacaagcgg atgctgcgta ttttccagga ggcgctggat ttgcaggaac
    16261 atatttcggt cagtttgcat cagccggaag aggtgcaaaa actggtcgag cgtagccatg
    16321 cggaagaagt tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg
    16381 atgacattct ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac
    16441 tggaaaaaat cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt
    16501 tcagccgcat cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg
    16561 ccgataaaca gcggcgaatg ccgttgcttc cggcactgaa aagttatctg tcactaaagt
    16621 ctccggcgct acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg
    16681 gcaccgtgct gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac
    16741 aaaatggcta tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg
    16801 ggttaatcat tgcgggcgta gcgctgcact ttaaaattcc cgaaagttac accctgacgt
    16861 tgatgctgat taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcaa
    16921 cggtcggttt tactattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc
    16981 agtacatcct tccacgtctt atcgatacca ttattggttg tttgattgct ttcggtggta
    17041 ccgtctggct gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgatgctt
    17101 tagaagccta tcaggaagcg attcgcttga ttctgagcga ggacccgcaa ccaacgccac
    17161 tggcctggca gagaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc
    17221 aggcgatgca ggaaccggca ttcaacagcc attatctggc agatatgaaa ctgtgggtaa
    17281 cgcacagcca gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc
    17341 gggcattgcc acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc
    17401 gttgtcagca gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg
    17461 atgcgccgga gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc
    17521 gggttattgg ccatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac
    17581 cgcatcacgg gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tacaggctaa
    17641 accatcggat aaggcattca cgccgcatcc gacatatttt gcctgatacg tcttatttac
    17701 gccccaacca ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca
    17761 tcttccacca ccagcgacgg tgctaaacgc atcacatccg gtccggcatt cagcaccatt
    17821 acgccaacct ctgcacccgc atacaggaaa tcacgcgccc gacctttgta gtgtggtttc
    17881 agctccgcgc caattaacag ccccataccg cgaatatcgc taaacacatc gtactgctga
    17941 tcgatcttct gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact
    18001 tcaggggtat tgatgatatc aaacaccgcg cccgctactg cacaggccag aggattaccg
    18061 ccgtaggtgg aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc
    18121 atggcgctaa tcgggaagcc gccgcctaac gctttcgcag aagtcagaat atccggcgtc
    18181 acaccgtagt acctgtaagc aaacaaatcg ccagtccgcc ccatcccgca ctgcacttca
    18241 tcaaacacca ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct
    18301 ggcgtcgctg ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg
    18361 tgatcatcca tcaccgcttt cactgcatgg agatcgttaa agggaacgtg gatgatgtct
    18421 gccggtttcg gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac
    18481 agcgagcgac catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga
    18541 cgcacacagg cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag
    18601 ttcataaaca ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc
    18661 gccggttcat tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac
    18721 gcgttcacca gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa
    18781 tcgacatact ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaata
    18841 aactctgccg gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct
    18901 gtttgttcaa ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa
    18961 tcacaaaata tgcataaaaa atcactgaat ggcaatcgga aatcagagat gcaggaaatt
    19021 agccagcagt tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc
    19081 cagatcccac tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac
    19141 gtcaaagcac gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt
    19201 aagtggattt gccagccccc gaaatacgcc ctcaccgcta tgtgtaatcg gcgaggtttt
    19261 gccgtgcatg acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg
    19321 atgaccgagg cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag
    19381 ggagatcccg gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt
    19441 aagggcgtcg atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc
    19501 cagttcacaa aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag
    19561 gatcatggcg gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc
    19621 ttaccacttt gctaaatatc atctgcagtg cggtgagatc ccccattgcc ccactctgat
    19681 ttgcctgatt ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa
    19741 tcgccagttg ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca
    19801 gcacgttgat ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc
    19861 caaccagata accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat
    19921 aagcaaagtg gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg
    19981 cccagtcgaa aatatcctgg tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc
    20041 gcaccagcgg accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct
    20101 gttccagccg ctgctgctgg cggatgttca gccggttacg catgatatca aggccaggat
    20161 aaagatacag atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca
    20221 aggcgcgcta aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga
    20281 cggcgactgg cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta
    20341 tcggtgagtt tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg
    20401 gcaaatccgg gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc
    20461 attacggcag gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg
    20521 gaccaacatc atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca
    20581 ctttaccaaa taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg
    20641 caacgttgat aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga
    20701 tggtgccacg cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt
    20761 ttttctgctg catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc
    20821 ggtgaaaggt agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag
    20881 acactggcgc tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca
    20941 atacgtgcgg gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcagaaa
    21001 gagcgaaaac agcagccatc gccgccaggg tcgatttgaa catgagattt ccttaacaaa
    21061 cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca aggccatccc
    21121 tttacctaat tttacgtatc tgaaatatat gtaacctgac gatcactttt tattccgtga
    21181 tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat ttaaacagat
    21241 cataaaatca cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt ttatttttga
    21301 acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta gctttctctc
    21361 ctacgcactg actggtgcgt tggttattgt caccgggatg gtgatgggaa atatcgccga
    21421 ttatttcaat ctgcctgttt ccagtatgag taacaccttc accttcctca acgccggcat
    21481 tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga aaacgcagtt
    21541 acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca gccacagcct
    21601 ggcgctgttc tcgacggcga tgttcattct cggggtggtc agcggcatta ctatgtcgat
    21661 tggtacattc ctgataacac aaatgtatga agggcgtcag cgcggttccc gcctgttatt
    21721 tactgactcc ttcttcagta tggctgggat gattttccca atgatcgccg cgtttctact
    21781 ggcgcgcagc attgagtggt actgggttta tgcctgcatc gggctggtgt atgtcgctat
    21841 ttttattctg accttcggct gtgagttccc ggcactgggc aaacgtgcac caaaaacgga
    21901 tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg ttgcggcgct
    21961 gtgctacatc ctcggtcagt taggttttat ctcctgggtg cctgagtatg ccaaaggcct
    22021 gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt catacatggt
    22081 cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcaacgca ttctgaccgt
    22141 actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac cagtacatat
    22201 ggcgtggtca attctcgctc tgggcttctt ctccagcgcg atctatacca ccatcatcac
    22261 cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg tcctgacctg
    22321 cggaaccatc ggtactatgt tgacctttgt ggttaccggc acgattgttg aacatagcgg
    22381 tccgcaggcg gcactgctta cagcaaacgg tctgtacgct gtcgtctttg tgatgtgctt
    22441 cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc attaattgct
    22501 aatgccggac ggcactatcg tcgtccggcc ttttcctctc ttcccccgct acgtgcatct
    22561 atttctataa acccgttcat tttgtccatt ttttgcacaa acatgaaatg tcagacaatt
    22621 ccgtgactta agaaaattta tacaaatcag caatataccc cttaaggagt atataaaggt
    22681 gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa
    22741 agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg
    22801 ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg atattaccgt
    22861 tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcatctt acttctctca
    22921 ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa
    22981 agttctggtc ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga ttcactccag
    23041 cgccggacgt accgtttttt atgacaagct gatcatggca accggttcct acccgtggat
    23101 cccaccaatc aaaggttctg atactcagga ctgctttgtc tatcgcacca ttgaagacct
    23161 caacgccatt gaatcctgcg cccgtcgcag caaacgcggt gccgttgttg gtggcggcct
    23221 gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc acgttatcga
    23281 atttgcccct atgctgatgg cggaacagct tgatcagatg ggtggcgagc agctgcgtcg
    23341 caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa aacacccttg agattgtgca
    23401 ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac tggaagtcga
    23461 ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct
    23521 ggacgttgct ccgcgtgggg ggattgtcat taatgattcc tgccagactt ccgatccgga
    23581 tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc
    23641 aggctacaaa atggcgcagg tcgccgttga ccatattctg ggtagcgaaa acgcctttga
    23701 aggtgctgac cttagcgcca agctgaaact gctgggcgta gacgtaggtg gtattggtga
    23761 tgcgcacggt cgcacgcctg gcgcacgtag ctatgtttac ctcgacgaaa gtaaagagat
    23821 ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg
    23881 cgataccagc gactacggca acctgctgca actggtgctg aacgctatcg agctgccgga
    23941 aaacccggat tctctgattc tgccggcgca ctcgggtagc ggtaagccgt ctatcggtgt
    24001 tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacta aaggtgatct
    24061 gattgctgcc atcaacaaag gctgccacac agttgcggcg ctgaaagctg aaaccaaagc
    24121 gggtactggc tgcggtggct gtatcccgct ggtcactcag gtactgaacg cggaactggc
    24181 gaaacagggc atcgaagtta acaacaacct gtgcgaacac tttgcttatt cgcgtcagga
    24241 actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca
    24301 cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc ggttcgctgc tggcctcctg
    24361 ctggaacgaa tacattctga agccggaaca tactccgctg caggattcta acgacaactt
    24421 cctcgctaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc cgggcggtga
    24481 aatcaccccg gaagggctga tggcagtggg tcgtatcgcg cgtgaattta atctctacac
    24541 caaaatcacc ggctcccagc gcctggcgat gtttggcgca cagaaagacg atctgccgga
    24601 aatctggcgt cagctgattg aagctggctt cgaaaccggt catgcctatg cgaaagcact
    24661 gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggtgttg gcgatagcgt
    24721 cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa
    24781 gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagggtaaag acgtgggtat
    24841 tatcgccact gaaaaaggct ggaacctgta tgtttgcggt aacggcggca tgaaaccgcg
    24901 tcatgcggat ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg
    24961 cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcgc cgtggttaga
    25021 aaacctcgaa ggcggcatcg attacctgaa agcggtgatc attgacgaca aactggggct
    25081 gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac
    25141 tgaaacggtc aatacaccgt ccgcgcagac tcgcttcaaa cacttcatca acagcgacaa
    25201 gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg caacgccgta
    25261 tgaacgtatc ccggtaactc tggtggagga caacgcatga gccagtggaa agacatctgc
    25321 aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcac tgttaggtga caagcaggta
    25381 gcgattttcc gcccgtatca cagcgaccag gtgtttgcga tcagcaacat tgatccgttc
    25441 ttcgagtcca gcgtgctatc acgcgggctg attgcggaac accagggcga gctgtgggtc
    25501 gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat ggaagacgaa
    25561 cagttttccg tcaaacatta cgaagcacga gtgaaagacg gcgtggtgca gctgcgcggt
    25621 taatgtttta acgggaggcg caatgcctcc cctttttgca tggtcctgta atgaccttcg
    25681 gtatattgca ggacattttt taaacttttt gttttatttt ttgtttttat tttttaaagg
    25741 ataatcaaat gtttacagac actattaata agtgtgcggc taatgctgcg cgcattgcac
    25801 gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcatatg
    25861 tgggtcttgg gatcatcctg attttcacgc tcggtaattt gctcgacccg tccgtacgtc
    25921 cgctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtgattatc gccggttctg
    25981 aactgttcac cggacacacc atgttcctca cctttggggt aaaagcgggc agcatcagcc
    26041 acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtc ggttccgtct
    26101 tcgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg
    26161 ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg
    26221 cattgtgtaa ctggctggtt tgcctggcaa tctggatggc gctgcgcact gaaggggcgg
    26281 cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact
    26341 ctatcgctaa catgacgctg ttcgcgctct cctggttcgg caaccacagc gaagcctaca
    26401 cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg
    26461 ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg
    26521 acaaatttaa tcaaactgaa acagctgccg gttaattact aaggggtttt tacgtggatc
    26581 atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg
    26641 tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat
    26701 tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag
    26761 ggccatttga tgaaagcctt ctcgacacct gctggctggc gattgcagcg acggatgatg
    26821 acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtag
    26881 ttgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcaccgctaa
    26941 tggtagcggt ctcctctggc ggcacctctc cggttctggc gcgcctgttg cgcgaaaaac
    27001 tcgaatcact gctgccgttg catctgggcc aggtggcgaa atatgccggg caattacgcg
    27061 ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg ccgtttctgg gagaaattgt
    27121 tcgttaacga ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc attactgaaa
    27181 cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg tgaagtggtg ctggttggtg
    27241 caggtccggg cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag
    27301 atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg
    27361 ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg tgtaccgcag gaagagatta
    27421 accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg
    27481 atccgtttat ttttggccgt ggtggcgaag agctggaaac actgtgcaac gcgggtattc
    27541 cgttctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc
    27601 cactcacgca tcgcgattat gcccagagcg tacgcttaat taccggacac ttaaaaaccg
    27661 gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttctatatgg
    27721 ggttgaatca ggccgcgact attcagcaaa agctgattga acacggtatg cctggcgaaa
    27781 tgccggtggc aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt gacggtacgc
    27841 tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc atcgctaatt attattggtc
    27901 gggttgttgg cctgcgcgat aaactgaact ggttctccaa tcattaattt aacccgggcc
    27961 agagaattct ggccttctta atgactcttt ttatatattc agcaaataaa acataacccc
    28021 ttaataaata ttatctgcta atgggttata ttcaccgtta cagcgattta caaaattaag
    28081 tcattcgctc actgacgcca attaaatatt cctgtatcgt tcttaacgag cagtctgctt
    28141 atattcatat aatcaatgaa tattaattaa tatataatac atagggaatg taaatgaaca
    28201 aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc
    28261 atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta
    28321 tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc
    28381 taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg
    28441 tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttta taactacatc
    28501 cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat cacataacat
    28561 aatatgttgt tatattcatc atgcattgtc atgttacctt ttaaatggct gcaaactctc
    28621 ccctacaacg tattggacaa gaaaaaggta tcgctatggg aagccaggaa ctccaacgca
    28681 agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt
    28741 ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt
    28801 ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg
    28861 cttatccgga aaatggcgca gattatgttt atctgaaaaa tgccggaagc cgaccgctgg
    28921 ctttcctctc cggctgggcc agcttctggg ccaacgatgc gccgtcattg tcgattatgg
    28981 cgctggcgat tgtcagcaat cttggctttt taacgcctat cgatccgttg ctcggtaaat
    29041 ttatcgccgc cggattaatt atcgccttta tgttgctaca cctgcgctcc gttgaaggcg
    29101 gcgcagcgtt tcagacgcta attaccatcg ccaaaattat cccgttcact atcgtcattg
    29161 gccttgggat cttctggttt aaagcggaga attttgccgc ccttaccacc actgcgattg
    29221 gcgcaacggg cagctttatg gcgctgctgg cggggatctc tgccaccagt tggtcgtata
    29281 ccggcatggc ctctatctgt tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc
    29341 cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg
    29401 tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg
    29461 ccctgaccta gatccccgca ctcggcagca ccgctgggct ctttgttgcc atcacggcga
    29521 tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg
    29581 cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgtgcatccg aaatacaaca
    29641 cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg
    29701 atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct
    29761 tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg
    29821 ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct
    29881 ttgtctgggc accgattccc ggccttatct gcgccgccat cgttattgct actggtctgc
    29941 ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc
    30001 tgaagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa
    30061 tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acgccatcgt
    30121 ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gctcgccact
    30181 caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc
    30241 catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt
    30301 gattggcgtt tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg
    30361 ggcctgcgga gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc
    30421 ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg
    30481 ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat
    30541 caaaaacgat ctcaagcagt tgccgaatgc gctcggtcat ctggtacgca cctgggaaga
    30601 aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc
    30661 gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg
    30721 gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga
    30781 accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg
    30841 cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat
    30901 ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct
    30961 ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg
    31021 tctggtggaa tattaatccc tctcccggcc cggcaacggg ccggatttac gcaaggagtt
    31081 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
    31141 ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg
    31201 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
    31261 atcagatgcc gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc
    31321 tgggcgatga acatatgcgt cgtgaaagcc tggacatgat caagctggcg atagatatgg
    31381 caaaagagat gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc
    31441 cgcctaacgt tatctggggg cggctggcag agaacttaag cgaactgtgt gagtacgcgg
    31501 aaaacatcgg catggatctg atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt
    31561 gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca
    31621 tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac
    31681 tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt
    31741 acattcctgg agaaggcaaa atgccgctgc gggaactgat gcgcgatatt attgatcggg
    31801 gctatgaggg ttactctacg gtggagctgg tgacgatgta tatgaacgag cccagactct
    31861 atgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac
    31921 cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc
    31981 tggcggtaat gcggtcaatg tggcggtgta ctgcactcgc tacggcatac agccgggatg
    32041 cattacctgg gtgggtgacg atgactacgg cacaaagctg aagcaggatc tcgcccgcat
    32101 gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga
    32161 actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc
    32221 cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg
    32281 gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga
    32341 cttctccgac aagtgggaca gcccgctctg gcagacactg gtgccgcatc tcgattttgc
    32401 ctttgcctcc gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc
    32461 ccgtggcgca ggaacagtga ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg
    32521 cgcgcagttc tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg
    32581 agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc
    32641 gatggcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt
    32701 ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta
    32761 taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc
    32821 tcatcaactc ctctacgcta ccgtccgcca gcggctgctg gatgatatcg cgcagggggt
    32881 ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacacaat ataacgtcag
    32941 ccgcattacc attcgcaaag ccatcagcga cttagtggca gacggcgtac tgatccgctg
    33001 gcagggaaaa ggcacctttg tacaaagcca gaaagttgaa aacgccctgc ttactgt
//
LOCUS       DAJTMX010000032        31468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTMX010000032 DAJTMX010000000
VERSION     DAJTMX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.032"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO145_001529"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO145_001529"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5466687.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO145_001530"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO145_001530"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5466688.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO145_001531"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO145_001531"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5466689.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO145_001532"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO145_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5466690.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO145_001533"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO145_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5466691.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO145_001534"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO145_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5466692.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO145_001535"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO145_001535"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5466693.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO145_001536"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO145_001536"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5466694.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO145_001537"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO145_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5466695.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO145_001538"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO145_001538"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5466696.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO145_001539"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO145_001539"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5466697.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO145_001540"
     CDS             8212..9075
                     /locus_tag="OO145_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5466698.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO145_001541"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO145_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5466699.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO145_001542"
     CDS             10411..11028
                     /locus_tag="OO145_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5466700.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO145_001543"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO145_001543"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5466701.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO145_001544"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO145_001544"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5466702.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO145_001545"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO145_001545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5466703.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO145_001546"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO145_001546"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5466704.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO145_001547"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO145_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5466705.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO145_001548"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO145_001548"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5466706.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPSVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO145_001549"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO145_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5466707.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO145_001550"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO145_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5466708.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO145_001551"
     CDS             21859..23568
                     /locus_tag="OO145_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5466709.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO145_001552"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO145_001552"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5466710.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO145_001553"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO145_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO145_001554"
     tRNA            27625..27719
                     /locus_tag="OO145_001554"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO145_001555"
     CDS             27991..29202
                     /locus_tag="OO145_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5466711.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO145_001556"
     CDS             29614..30657
                     /locus_tag="OO145_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5466712.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31468
                     /locus_tag="OO145_001557"
     CDS             31172..>31468
                     /locus_tag="OO145_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466713.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPAL"
BASE COUNT         7888 a         8294 c         7806 g         7480 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatagg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 agcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttg
//
LOCUS       DAJTMX010000033        30435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTMX010000033 DAJTMX010000000
VERSION     DAJTMX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.033"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1544
                     /locus_tag="OO145_001558"
     CDS             <1..1544
                     /locus_tag="OO145_001558"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5466714.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
     gene            1703..2262
                     /locus_tag="OO145_001559"
                     /pseudo
     CDS             1703..2262
                     /locus_tag="OO145_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2263..3912
                     /locus_tag="OO145_001560"
     CDS             2263..3912
                     /locus_tag="OO145_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5466715.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3917..4693
                     /locus_tag="OO145_001561"
     CDS             3917..4693
                     /locus_tag="OO145_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5466716.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4968..7817
                     /gene="rcsC"
                     /locus_tag="OO145_001562"
     CDS             4968..7817
                     /gene="rcsC"
                     /locus_tag="OO145_001562"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5466717.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8017..8667)
                     /gene="rcsB"
                     /locus_tag="OO145_001563"
     CDS             complement(8017..8667)
                     /gene="rcsB"
                     /locus_tag="OO145_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5466718.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8684..11356)
                     /gene="rcsD"
                     /locus_tag="OO145_001564"
     CDS             complement(8684..11356)
                     /gene="rcsD"
                     /locus_tag="OO145_001564"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5466719.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12094..13215
                     /gene="ompC"
                     /locus_tag="OO145_001565"
     CDS             12094..13215
                     /gene="ompC"
                     /locus_tag="OO145_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5466720.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13327..14382
                     /gene="apbE"
                     /locus_tag="OO145_001566"
     CDS             13327..14382
                     /gene="apbE"
                     /locus_tag="OO145_001566"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5466721.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14456..15520
                     /gene="ada"
                     /locus_tag="OO145_001567"
     CDS             14456..15520
                     /gene="ada"
                     /locus_tag="OO145_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5466722.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15520..16170
                     /gene="alkB"
                     /locus_tag="OO145_001568"
     CDS             15520..16170
                     /gene="alkB"
                     /locus_tag="OO145_001568"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5466723.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16246..17889
                     /gene="yojI"
                     /locus_tag="OO145_001569"
     CDS             16246..17889
                     /gene="yojI"
                     /locus_tag="OO145_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5466724.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18107..18466
                     /locus_tag="OO145_001570"
                     /pseudo
     CDS             18107..18466
                     /locus_tag="OO145_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18700..19731
                     /locus_tag="OO145_001571"
     CDS             18700..19731
                     /locus_tag="OO145_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5466725.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(19853..20341)
                     /gene="eco"
                     /locus_tag="OO145_001572"
     CDS             complement(19853..20341)
                     /gene="eco"
                     /locus_tag="OO145_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5466726.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(20476..20640)
                     /locus_tag="OO145_001573"
     CDS             complement(20476..20640)
                     /locus_tag="OO145_001573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466727.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            20749..21243
                     /gene="napF"
                     /locus_tag="OO145_001574"
     CDS             20749..21243
                     /gene="napF"
                     /locus_tag="OO145_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5466728.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21233..21496
                     /gene="napD"
                     /locus_tag="OO145_001575"
     CDS             21233..21496
                     /gene="napD"
                     /locus_tag="OO145_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5466729.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21493..23979
                     /gene="napA"
                     /locus_tag="OO145_001576"
     CDS             21493..23979
                     /gene="napA"
                     /locus_tag="OO145_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5466730.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            23986..24681
                     /gene="napG"
                     /locus_tag="OO145_001577"
     CDS             23986..24681
                     /gene="napG"
                     /locus_tag="OO145_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5466731.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24668..25531
                     /gene="napH"
                     /locus_tag="OO145_001578"
     CDS             24668..25531
                     /gene="napH"
                     /locus_tag="OO145_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5466732.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25528..25977
                     /gene="napB"
                     /locus_tag="OO145_001579"
     CDS             25528..25977
                     /gene="napB"
                     /locus_tag="OO145_001579"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5466733.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            25987..26589
                     /gene="napC"
                     /locus_tag="OO145_001580"
     CDS             25987..26589
                     /gene="napC"
                     /locus_tag="OO145_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5466734.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26608..27225
                     /gene="ccmA"
                     /locus_tag="OO145_001581"
     CDS             26608..27225
                     /gene="ccmA"
                     /locus_tag="OO145_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5466735.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27222..27884
                     /gene="ccmB"
                     /locus_tag="OO145_001582"
     CDS             27222..27884
                     /gene="ccmB"
                     /locus_tag="OO145_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5466736.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27926..28663
                     /gene="ccmC"
                     /locus_tag="OO145_001583"
     CDS             27926..28663
                     /gene="ccmC"
                     /locus_tag="OO145_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5466737.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28660..28869
                     /gene="ccmD"
                     /locus_tag="OO145_001584"
     CDS             28660..28869
                     /gene="ccmD"
                     /locus_tag="OO145_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5466738.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28866..29345
                     /gene="ccmE"
                     /locus_tag="OO145_001585"
     CDS             28866..29345
                     /gene="ccmE"
                     /locus_tag="OO145_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5466739.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29342..>30435
                     /gene="ccmF"
                     /locus_tag="OO145_001586"
     CDS             29342..>30435
                     /gene="ccmF"
                     /locus_tag="OO145_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5466740.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLV"
BASE COUNT         7013 a         7524 c         8475 g         7423 t
ORIGIN      
        1 taacctcgtc gctgaaaggt aaacctgttt ctgcgcagct aacggtaggc gtggtcgatg
       61 aaatgatcta cgcgctgcaa ccagaaatcg cgccgaatat cggcaaattt ttctatccgc
      121 tggggcgtaa caatgtgcgt accagctcca gtctgtcgtt tatcagctat gaccaggcgc
      181 tctccagcga gccggttgcg cctggcgcga ctaaccgcag cgagcggcga gtaaaaatgc
      241 ttgaacgtcc acggcgtgaa gaggtggata ccgcggcatg gatgccgtca ctcacaaccg
      301 ataaacaagg caaagcgtat ttcacgttcc tgatgcctga ttcgttaacc cgctggcgta
      361 tcaccgcgcg tgggatgaac ggcgacgggc tggtcgggca ggggcgtgct tatctgcgtt
      421 cggaaaaaaa tctctacatg aagtggagta tgccaacggt gtatcgcgtg ggcgacaaac
      481 cggcggcagg actgtttatc ttcagtcagc aggataacga accggtggcg ctggtgacta
      541 aatttgcagg cgctgagatg cgccagacgc tgacgctgca caaaggggcg aattatattt
      601 cgctgacgca gaatattcag caatctggct tgttaagtgc agaactgcaa caaaatgggc
      661 aagtgcagga cagcattagc acaaaactgt cttttgtgga caacagctgg cccgttgaac
      721 agcagaaaaa tgtcatgctc ggcggtggcg agaacgcgct gatgttgccc gagcaggcga
      781 gtaatatccg gctacaaagt agtgaaacgc cgcaggagat tttccgcaac aatcttgatg
      841 cgttagtcga tgaaccgtgg ggtggggtga tcaacaccgg tagccgtctg atcccgctca
      901 gtctcgcctg gcgttcgctt gccgatcatc aaagtgccgc cgctaacgac attcgtcaga
      961 tgattcagga taaccgtctg cggctgatgc aactggcggg gcccggagcg cgctttacct
     1021 ggtggggtga agatggcaat ggtgacgcct tccttacggc atgggcatgg tacgccgact
     1081 ggcaggccag ccaggcgctc ggcgtaacgc aacaaccgga atactggcag catatgctcg
     1141 acagttatgc cgagcaggcg gataatatgc cgttattgca tcgggcgctg gtgctggcgt
     1201 gggcacagga gatgaatctg ccgtgcaaaa cgttgttgaa agggggggat gaagctatcg
     1261 cccggcgcgg aactaaagat gaagatttct ctgaggaaga catccgcgat atcaatgaca
     1321 gcctgatcct cgatacaccg gaatctccac tggcagatgc ggtggcaaac gtcttaacca
     1381 tgacgttgct gaaaaaagcg cagttgaagt ccacggtgat gccacaggtt cagcaatatg
     1441 cgtgggataa agcggcaaac agcaatcagc cgctggcgca cacggttgtg ctgctcaata
     1501 gcggtggtga cgctacccag acggccgcta ttttaatggt ttgaccgctg agcaatccac
     1561 tattgagcgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa tgcctccagt
     1621 tgttttgcct gcgcctgcgg gcgcatgggc taaacataag ttaactggag ggggcgaaga
     1681 ctggcgttgg gttggttagg gtgtgccgga cattctctct tttggtgacg aattatcgcc
     1741 gcaaatgtgc aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg
     1801 gtgaccgttg aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact
     1861 ctgcagccgg tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg
     1921 cttaccagcg agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg
     1981 ggagctgacg ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa
     2041 cagcaggggc aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg
     2101 ccggtgaaag aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa
     2161 gggcaattcg ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt
     2221 gtcgcggcag ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc
     2281 gtctggctat tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg
     2341 gtgctgcgcg gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa
     2401 gtctcagcat tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt
     2461 tacgcctggc tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc
     2521 ccggaagagg tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta
     2581 cgttcctgtg ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg
     2641 caatactggc aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca
     2701 gaaacctcgg taacggttaa atcgctgctt gattcattag cgacgttacc tgcgcaaaac
     2761 aaagctcagg aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc
     2821 atgctcggta gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa
     2881 atccgtcagg gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc
     2941 agtggcacca gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca
     3001 gtgccgacgc aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg
     3061 ctgaagaaaa tcacagcgga aaagagtacg acggcagtaa aacccagcgt acttaatggg
     3121 cgctatcgtg tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg
     3181 accttactga gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac
     3241 gttgccagag ttctggatcg cgaggcgaaa agtacgccac ctgaggcagc gaaagccatg
     3301 actgtggcga tacgtacttt tttacagcaa aacgccaatc gggaaggtga ttgcctgacc
     3361 atccccgaca gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga
     3421 acgatgaccg cctggacgca ggagctcatc tatgcaggcg atccggttca ttatcatggc
     3481 agtcgcgcta ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg
     3541 gagcgttacg accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga
     3601 gctccacgct caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg
     3661 ccgcagtggc ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg
     3721 gtctgtcgtt tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt
     3781 cgtaacttct ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacttggcc
     3841 tttgatggtt atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta
     3901 ttaatggact aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc
     3961 tgtcgctcac agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca
     4021 tccggcagag ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt
     4081 caacatggca gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc
     4141 tgcggcgggt aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag
     4201 aattgaatcc gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt
     4261 gcaggtcatc agcccggatg ggcaaagccg gcaaaaaatg cagttttact caacgccggg
     4321 tacaggaact attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga
     4381 tctcgatctc catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct
     4441 gaaaaacagt ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc
     4501 catgccagca ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag
     4561 cgaaacggaa ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga
     4621 gaaacaggaa acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag
     4681 ttttgactgg tgatccatcc cttcacacaa cctggctctc tcttttttca gataagactg
     4741 ctgggcgatc tgccgacgcc aggatataac cattaacaag cgtcggcaga ttggtcattt
     4801 cttatttttc gtactgagaa ctgtccatct actccggctt tcctggttaa ggcaatggtt
     4861 tctaagcgat acgcattatt gcaatggtga cagaacattg cggtaacacg cttttaccgc
     4921 taccttaacc acactccatc ggtcacctga ggcggagctt ctcccctttg aaataccttg
     4981 cttcttttcg tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc
     5041 tctggctgtt gattgctttt tcatccgttt tttacatcgt taatgcgtta catcagcgag
     5101 aatcggaaat tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac
     5161 gcacctctga tgtgatgaaa gagcttaagt acatcgccga aaatcgctta tcggcagaaa
     5221 acggtgtgct ttccccgcgt ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc
     5281 tgtttgccga ctccgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat
     5341 tggcgtggtt tatgcgctac tggcgcgata atttttctgc ggcttacgat ctcaaccggg
     5401 tatttttaat cggcagtgat aacctctgca tggccaattt cggtctgcgt gatatgccag
     5461 tggaacgcga taccgcgttg aaagcgttgc atgaacgcat caataaatat cgaaatgcac
     5521 cacaagatga tagcggcagt aacctctact ggatcagcga aggtccgcgc ccttgcgtcg
     5581 ggtattttta cgcgttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg
     5641 tcgagcagac catccggatg gagaactttt tcttaccggg tacgttgccg atgggggtta
     5701 ccattcttga tgaaaatggt cataccctga tttcgcttac cggaccagaa agtaaaatta
     5761 agggcgatcc tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccggg
     5821 agctggtgct gaagaaaaat ctgccaccct catcgctaag catcgtgtat tcggtgccgg
     5881 ttgataaggt gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc
     5941 ttgccggagc tgcattgttt actctcgcgc ggatgtacga gcgacgtatt ttcattccgg
     6001 cggaaagcga cgccctgcga ctggaagaac atgagcagtt caatcgcaag attgtcgcct
     6061 ccgcgccagt gggtatctgc attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg
     6121 aactggcgca tacctatctc aatatgctta cgcatgagga ccgccaacga ctgacgcaaa
     6181 ttatctgtgg gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc
     6241 aaatcagctt cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg
     6301 atgtttcttc gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac
     6361 aggcgagcca gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg cgaacgccgc
     6421 tgtatggcat tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg
     6481 atcgtctggt gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata
     6541 ttctcgattt ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt gagttttcac
     6601 cgcgtgaagt gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt
     6661 taggcttgta ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc
     6721 gtttacagca ggtcatctcc aacctgttga gtaacgccat aaaattcacc gataccggct
     6781 gtatagtttt gcatgttcgc gctgatggcg attatctctc tatccgtgtt cgcgataccg
     6841 gcgtggggat tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggttggaa
     6901 cgggcgtaca gcgaaacttc caggggaccg gtctgggtct ggcgatttgt gaaaaactga
     6961 tcagcatgat ggacggcgat atctcggtag attcagaacc gggaatgggc agccagttta
     7021 ccgtgcgtat tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg gaagggttga
     7081 gtggtaaacg ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca
     7141 gtttgcagcg cagcggcatc gtcgttacaa catacgaagg gcaggaaccg actcccgaag
     7201 atgtgttaat cactgacgag gtagtgagta aaaaatggca gggcagagcg gtagtgacct
     7261 tctgtcgtcg ccatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg
     7321 tggctgctcc gcatgagcta ccggcattgt tggcgcgtat ttatttgatc gagatggaga
     7381 gcgacgatcc tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aacgacgata
     7441 tgatgattct ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg
     7501 gatcgttggg ctatcaatgt aaaaccgcga atgatggcgt cgatgcgctt aatgtactta
     7561 gtaagaatca tattgatatc gtgcttagcg acgtcaacat gccaaatatg gatggttacc
     7621 gcttgacgca acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta
     7681 atgcgttggc tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctctcga
     7741 agccggtaac gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga
     7801 aatcgcggga ttcgtaggac ggataaggcg tttacgccgc atccggcatt cagtgcaaat
     7861 gccagataag acactgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat
     7921 gggaatcgta ggccggataa ggcgtttacg ccgcatccgg caatcggtgc aaatgccaga
     7981 taagacacta acgcgtctta tctggcctac agatgattag tctttatctg ccggacttaa
     8041 ggtcacagaa gagagataat tcagcagggc gatatcgttc tcgacgccca gcttcatcat
     8101 cgcagatttc ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc
     8161 ggtcactagg aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg
     8221 cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt
     8281 aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg
     8341 tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt
     8401 gttcatagtc agaacaatga tcgacaggct tgggaaatgg cgcttgatgt acttgattaa
     8461 ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgcgc
     8521 atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt
     8581 cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc
     8641 ggcaataatt acgttcatat tgttcatgta ataggctacc ttgctacagc aagctcttga
     8701 cataactgtc aatgtcgctg atgtattttt ctattcctgg aacatccttc tcacgaatca
     8761 gatgttccag cgtttcacat aactgcttgc cgggtaccag atttagcatg gcaaatacgc
     8821 ctttaagacg atgagccgtt tgggctaacg cagcaaagtc actggttgct gcttcagtat
     8881 acagcctctt aacatcatcc ggtactgtgt ctacaaagag cgcataatag ccgctggcat
     8941 ggagttgcgc attttcatct ccgcccagag gcgattctgt cacctcttcc tgtgccagtt
     9001 gcacttcaat taattgtaag accgcttcct gcatagcgtt gctcatattg aagttgacgc
     9061 acaattgacc aggcccaatt tctcgtacgc cagactcatc atcgcttaaa agcaagccag
     9121 aggcagtaag attagacgga ttatccgtta aaaagatatc ataatcttga ctaattaatc
     9181 tttcatcggg tgtgatacag gttgcacccc aattttctaa caggcgagtg acaatattcc
     9241 gaatttctgc cgaagtaaca tccaccatta cgcagacatc atccagtaaa cgctcttctt
     9301 cctcttcaac ttccgggtca gcggcgagca ttttgatatg cacagagtag cgtgtaccaa
     9361 gcccatcccg cgttttgatg tttaaatgac cgcccagttt acgtgccagt tgatcgctca
     9421 gccagaatgc cagcgggtcc gcctttccat aacgatcgtt ttgggtctgg ttgataaacg
     9481 ggaagtgcaa attatccatt tcatgaatac ttacgccttc tcccgtgtcc agaatgcgga
     9541 atgtcaggcg gtcttcagag gactcatcct gatcaacctc aagggtgatt tttcccaatt
     9601 gcgttgaggt cacggcatat tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat
     9661 cgccgcggcg catatcgtgt gctttcagat gattgttaat cagcagttgc agacctttac
     9721 gcttgatggc aggcaacact gaaggcacaa cttcatcaat taaatcctgc acggagaaca
     9781 gcaccgtctc acttttccag ctatcatccg caagcatgtt cgctaactgt atttcttcga
     9841 ccagccgcac cagcacatct gcctgattcg ccagttgttt gctttccggg gcgttgagtt
     9901 tagccgcact ctccgccagg gactgtgcgg gttctttcag cgcatcgcca atgtttttca
     9961 taaaggtcat ccgcccctgc tggtttttct catacagacg ctgcgcctgc ttgagtttct
    10021 tgtttaccag cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcga
    10081 cctggctgcg gaacatgcgg atctcataca gctcgttatt gatcgtcgcc tgaataatcc
    10141 cctgatgctg ttccgccatg gtggtgatgt tttgcagatt cagatgcggc agcaaatgat
    10201 cggcaatttt gttacttatg acagtgcggt tcgattcctg atcgtgaacc agcaggccga
    10261 gcggcaacag tgagactatc tcttcattga ttgcccgtaa aatgcgcaat tcgttattga
    10321 ccgccgtgct ggggacgctt tctgtactgc ggctggagaa atggcggaat gtggtatagc
    10381 caaataacgc cagcgccagc aaaccgatgt tcagcagcag tggcagcaga atgttttgca
    10441 acgtatccag caataaggtg ccataaggaa cctgccagac caggcgcata tcggtagagt
    10501 tgagtgccga ggagatttca atcttcgtac tgttaaagtg gatactgacg ctatccgtcc
    10561 cttctttctc attatcattg tttcccgttg ccgtcgcgtc tggctcaagg cggaaactgt
    10621 ccagcggcat acccggtggg atcaaatcat taatcggcag atcaaaagcc acgaccgttg
    10681 ccagatgtcc cggctggttg aacgtggtac gcaaggtaaa gtaatgaccg ttctgccagg
    10741 ctaggcggcg catgttagaa aaactttcgc gttcatcgag ggcgttggcc tgttgtaaca
    10801 tttctgcacg acgtgaatca acaatgtcac tgacggtcga ttctttaaat ccggaggtga
    10861 gatctttaag gggtagggtt gagatcagca ccagactgtt atcctgacca ttcaggtaat
    10921 acatcgacca cggtacattt tctgcacccc acaatgtatc cagataagtg gacatccgct
    10981 gagtcatctc aagcgttgag ttgtcgtgag agccaaagat taacgcttcc gttttgcggc
    11041 gtggtttctc cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg
    11101 aggaggggga ggtcgtcgcg gcaatgttgt cgtagatctg cctggtcacg taacgccagg
    11161 tatcgacgcg cttttggatt gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca
    11221 gccaagcgtt aacggcactt tgtaccatta cccccatcgt caccagtaac acaatgatca
    11281 acagtaaaaa gaagcgggta atgctccccg gtaggagtga aaagcgggtc gtggccgttg
    11341 tctctttctg acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg atagggcagt
    11401 ataaagggta cagtgtgaaa ttccagactc ttacctgatt gatgattctc tattctcccg
    11461 tgatatttat catcctgtat ggggggaaat agatgtacaa gcgccatttt gctgtacaaa
    11521 taacctacaa aaagcccaac gaaattaatt gcaaataaac aaagattgct ggaaattatg
    11581 ctgatgttaa ttatttgtga aatagttaac aagcgttata gtttttctgt ggtagcacag
    11641 aataatgaaa agtgtgtaaa gaagggtaaa aaaaccgaat gcgaggcatc cggttgaaat
    11701 aggggtaaac agacattcag aaatgaatga cggtaataaa taaagttaat gatgatagcg
    11761 ggagttattc tagttgcgag tgaaggtttt gttttgacat tcagtgctgt caaatactta
    11821 agaataagtt attgatttta accttgaatt attattgctt gatgttaggt gcttatttcg
    11881 ccattccgca ataatcttaa aaagttccct tgtatttaca ttttgaaaca tctatagcga
    11941 taaatgaaac atcttaaaag ttttagtatc atattcgtgt tggattattc tgcatttttg
    12001 gggagaatgg acttgccgac tgattaatga gggttaatca gtatgcagtg gcataaaaaa
    12061 gcaaataaag gcatataaca gagggttaat aacatgaaag ttaaagtact gtccctcctg
    12121 gtcccagctc tgctggtagc aggcgcagca aacgctgctg aagtttacaa caaagacggc
    12181 aacaaattag atctgtacgg taaagtagac ggcctgcact atttctctga cgacaagtct
    12241 gtagatggcg accagaccta catgcgtctt ggcttcaaag gtgaaactca ggttactgac
    12301 cagctgaccg gttacggcca gtgggaatat cagatccagg gcaacagcgc tgaaaacgaa
    12361 aacaactcct ggacccgtgt ggcattcgca ggtctgaaat tccaggatgt aggttctttc
    12421 gactacggtc gtaactacgg cgtagtttac gacgtaactt cctggaccga cgtactgcca
    12481 gaattcggtg gtgacactta cggttctgac aacttcatgc agcagcgtgg taacggcttc
    12541 gcaacctacc gtagcaccga cttctttggt ctggttgacg gtctgaactt cgctgttcag
    12601 tatcagggca aaaacggtag cccggaaggt gaaggcatga ccaacaacgg tcgtgaagct
    12661 ctgcgtcaga acggtgacgg cgttggcggt tctattactt atgattacga aggcttcggt
    12721 atcggtgctg cagtttccag ctccaaacgt actgatgacc agaacttcgg tctgaaccgt
    12781 tatgatgagc gttacatcgg taatggcgac cgcgctgaaa cctacaccgg tggtctgaaa
    12841 tacgacgcga acaacatcta cctagctgct cagtacaccc agacctacaa cgcaactcgc
    12901 gtaggcaact tgggttgggc gaacaaagca cagaacttcg aagctgttgc tcagtaccag
    12961 ttcgacttcg gtctgcgtcc gtccctggct tacctgcagt ctaaaggtaa aaacctgggt
    13021 gtcatcaatg gtcgtaacta cgacgacgaa gatatcctga aatatgttga tgttggtgcg
    13081 acctactact tcaacaaaaa catgtccacc tacgttgact acaaaatcaa cctgctggac
    13141 gacaaccagt tcactcgtga cgctggcatc aacactgata acatcgtagc tctgggtctg
    13201 gtttaccagt tctaatctcg atggatatcg aacaaagggc ctgcgggccc ttttttcatt
    13261 gttttcagcg tacaaacaca gttttttggt gtactcttgc gaccgttcgc atgaggataa
    13321 tcacgtatgg aaataagctt tacccgcgtg gcactgctgg ttgccgcgct cttctttgtt
    13381 ggttgcgatc aaaaaccaca acccgccaaa acccacgcta ctgaagttac cgttcttgaa
    13441 ggcaaaacca tgggtacctt ctggcgtgcc agcatcccgg gcattgatgc caaacgcagt
    13501 gccgaactta aagaaaagat tcagacccag ctggacgccg acgatcagct gctttcgacc
    13561 tataaaaaag attccgcgct gatgcgcttt aacgactcgc aaagtttgtc gccgtggccg
    13621 gtaagtgaag cgatggccga tatcgtcacc acctcgctgc gcattggggc aaggaccgat
    13681 ggtgcgatgg atattaccgt cgggccgctg gtgaatctgt ggggctttgg cccggaacaa
    13741 cagccggttc aaattccgag ccaggaacag atcgatgcga tgaaagccaa aaccggctta
    13801 cagcacctga cggtcatcaa tcagtcgcat cagcaatatc tgcaaaaaga cctgccggat
    13861 ttatatgtcg atctctccac cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg
    13921 atggagcagg aagggatttc ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt
    13981 ggtatgaacg gtgaaggcca gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa
    14041 aacgcggttc aggctgtggt ggatatcaac ggtcatggga ttagcacctc tggcagctac
    14101 cgtaactatt acgaactgga cggcaaacgt ctttcccatg ttatcgatcc gcaaaccggg
    14161 cgtcccatcg aacacaatct ggtatccgtg acggtgattg ctccgacggc gctggaagcc
    14221 gatgcctggg atactggctt gatggtactc gggccggaga aagccaaaga ggttgttcgc
    14281 cgggaagggc tggcggttta tatgatcacc aaagaaggcg atagctttaa aacctggatg
    14341 tcaccacagt ttaaaagctt ccttgtcagc gaaaaaaatt aaagcgcaag attgttggtt
    14401 tttgcgtgat ggtgaccggg cagactaaag gctatcctta accagggagc tgattatgaa
    14461 aaacgccaca tgcttaactg acgatcaacg ctggcaatct gtcttagccc gcgacccgaa
    14521 tgccgacggc gaattcgttt tcgccgtgcg caccacgggt atcttttgcc gtccttcttg
    14581 ccgcgccaga catgccttgc gggaaaatgt ctccttctac gcaaatgcca gcgaggcact
    14641 cgccgccgga tttcgcccct gcaaacgttg tcagccagac aaagccaatc cccggcaaca
    14701 tcggttggat aaaatcaccc acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct
    14761 ggaagcctta gccgaccagg tggcgatgag tccatttcat ctacatcggt tgtttaaagc
    14821 tactaccgga atgacgccta aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga
    14881 atcgctggcg aaaggggaga gcgtgacgac gtctattctt aacgccggat tccccgacag
    14941 cagcagttac taccgcaaag ccgatgaaac gctgggcatg acggctaaac aattccgtca
    15001 cggtggcgaa aatctggcgg tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct
    15061 ggtggcagaa agcgagcggg ggatttgcgc gatattgctg ggcgatgatg acgcgacatt
    15121 aatcagcgag ttgcagcaga tgtttcccgc tgccgacaac gcgcctgccg atctgacgtt
    15181 tcagcaacat gtgcgtgaag tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt
    15241 accgctggat attcgcggca ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat
    15301 accttgtggt gaaaccgtca gttatcagca actggctaac gccatcggca aaccgaaagc
    15361 ggtacgggcc gttgccagcg cctgtgccgc caacaagctg gctatcgtaa taccctgtca
    15421 tcgggtggtc cgtggtgatg gcacactttc cggttaccgc tggggcgtgt cgcgtaaagc
    15481 gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa tgttggatct gtttgccgat
    15541 gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa ttttacggcg ttttgctttt
    15601 aatgctgcgg agcaactaat ccgcgatatt aatgacgttg ccagccagtc gccgtttcgc
    15661 cagatggtcg ctccaggcgg atataccatg tcggtggcga tgaccaactg tgggcatctg
    15721 ggctggacga cccatcggca aggttatctc tattcgccca ttgatccgca aacaaataaa
    15781 ccgtggcccg ctatgccaca aagctttcat gatttatgtc aacgtgcggc tacggcggcg
    15841 ggctatccag atttccagcc agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa
    15901 ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg cgccaattgt ttctgtttct
    15961 ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac gtaatgatcc gctcaaacgt
    16021 ttgttgttgg aacatggcga tgtggtggta tggggcggtg aatcgcggct gttttatcac
    16081 ggtattcaac cgttgaaagc ggggtttcat ccactcacca ccgactgccg ctacaacctg
    16141 acattccgtc aggcaggtaa aaaagaataa aaataagaat tattattgct gtgcgcgcga
    16201 agatgtttaa actgcgggct gtaattcatt gtccgggttt tctgcatgga acttcttgta
    16261 cttgtctggc ggcagtatcg ctggccattt atcagtgtga tggcgctaag cctcgccagt
    16321 gcggcattag gcattggctt aattgctttt atcaatcagc gccttatcga aacggcggat
    16381 accagtctgc tggtgttacc ggagtttctg ggattattgc tgctgttgat ggcagtcact
    16441 ctcggatcgc aactggcgct caccactttg gggcatcact tcgtttaccg actgcgtagt
    16501 gaatttatca agcggattct ggatacccac gtcgagcgca ttgaacaact cggtagcgcc
    16561 tcgttgctgg cgggattaac cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctg
    16621 ccggaactgg ggcaggggat cattctcact atcggttcat cggcgtatct gtggatgctg
    16681 tcgggcaaaa tgttgctggt aacggctatc tggatggcga tcaccatctg gggcggtttt
    16741 gtgctggtgg cgcgggtgta caaacatatg gcgaccctac gtgaaaccga agacaagctg
    16801 tacacggatt ttcaaactgt acttgaaggg cgcaaagagc tgactctgaa ccgggaacgc
    16861 gccgagtatg tgtttaacaa cctctacatc cccgatgcgc aagagtatcg ccatcatatt
    16921 attcgcgcag acaccttcca tcttagtgcc gtgaactggt caaacatcat gatgctgggc
    16981 gctatcggcc tggtgttctg gatggcgaac agtctcggtt gggctgatac caacgttgct
    17041 gcgacctatt cgttgacgct gttattcctg cgtacgccgc tgctttcggc ggttggcgca
    17101 ttgccgacgc tgctgacggc gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg
    17161 cctttcaaag ctgagtttcc gcgcccgcag gcgtttccca actggcaaac gctggagctg
    17221 cgtaacgtga cgtttgctta tcaggataac gcgttttccg ttggtccgat taatctcacc
    17281 atcaaacgtg gcgagctgct gtttctgatt ggcggtaacg gtagcggaaa atcgacgctg
    17341 gcgatgttgt tgacgggctt gtatcagcca caaagcggcg aaatcttgct ggatggcaaa
    17401 cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt tttcggcagt gtttaccgat
    17461 gtctggctgt ttgatcaact gctggggccg gaggggcaac ccgctaaccc gcaactggtt
    17521 gagaagtggc tggcgcagct gaaaatggct cataagcttg agttaagcaa cgggcgtatt
    17581 gttaacctga agttatcaaa agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca
    17641 gaagaacgcg atattatcct gctggatgaa tgggcggtgg atcaggatcc acacttccgt
    17701 cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga tgggtaaaac tattttcgct
    17761 atcagtcatg atgatcatta ctttatccac gccgaccgcc tgctggaaat gcgcaatggg
    17821 caacttagcg agctgacggg cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg
    17881 acggcataat attacgacgc tgacctggcg gactgctgcc gtcaggtcaa tatgctaaaa
    17941 atcccttcat ttctaataac cctataatta atcaacgaaa ttataatgtt tctaaaatta
    18001 gaatataatt tataaacatt atttaaatgt tgttacttaa gtgttaccgt tgatgccgcg
    18061 caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga aaaaagtgac
    18121 tgccatgctc ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa
    18181 agcgtccgag gagcaggaaa ctgatgtact gttgattggc ggcggcatta tgagcgccac
    18241 gttggggacc tattgacgcg agctggagcc tgaatggtcg atgaccatgg tggagcgcct
    18301 ggaggggggc gcgcaggaga gttcgaacgg ctagaacaac gccggaaccg ggcattctgc
    18361 actgatggaa ctgaactaca ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc
    18421 agtcgccatt aacgaagcat ttcagatttc ccgccagttc tgggcgcacc aggtcgagcg
    18481 cggcgtgctg cgtactccgc gttcatttat caataccgtt ccgcatatga gctttgtctg
    18541 gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct
    18601 gtttcgcggt atgcgttact ctgaagatca cgcgcagatc aaagagtggg caccgttagt
    18661 gatggaaggg cgcgatccgc aacagaaatt ggcactgatg tgaactatgg tgagatcacc
    18721 cgccagttaa ttgcttcctt acagaagaaa tctaacttct cgctgcaact cagcagcgaa
    18781 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
    18841 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
    18901 ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
    18961 ggcggacagt tccttgtttc ggaaaatcca gacgtggtta atcgccatct ggcgaaagtt
    19021 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt
    19081 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
    19141 aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
    19201 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
    19261 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaagcgaa aaaagaggac
    19321 tggcgtttgt ggcaagcggg gcagcgtgtg cagattatca agcgtgatgc cgagaaaggt
    19381 ggcgtactgc gtctgggtac cgaagtggtc agtgaccagc aagggaccat tgcggcgctg
    19441 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttgaatct actggaaaaa
    19501 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
    19561 tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt gcagtacacc
    19621 agcgaagtgc tggggctgaa atacgacaaa ccgcaagcag cagatagtac gccgaaaccg
    19681 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtggc
    19741 gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt tagatgttgg
    19801 atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca gtttagcgaa
    19861 cttccgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca tctacattgt
    19921 ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca gcatcgccca
    19981 gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc atcgtggaaa
    20041 ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc agcgttttgc
    20101 tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta
    20161 acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc acctgacgct
    20221 tcatgcctgt ttcagcttgt ggataaggcg cgattttttc cagtggctgg acgctttctg
    20281 ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac ttcaggtaga atggtcttca
    20341 tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg aaggttaaca
    20401 tattcatcag atttattaat ttgctttatg aataacttaa agaacattca gacaatttta
    20461 cctgctcttt tagtgttaaa ttctgatgag ggagcaaacc agcagaaata tctctggtga
    20521 attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag cccgtttttg
    20581 ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg gggtatgcat
    20641 ctttgacaca tcctttaata tcttagcggc tataaaaatg gcttattaat tatgcggtct
    20701 atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt gaagattgat
    20761 gcatcccgtc ggggcatact cactggttgc tggcgcaaag ccagtaacgg tatccgtccg
    20821 ccctggtccg gtgatgaatc tcattttctg acccattgca cccgctgcga cgcctgtatt
    20881 aatgcttgtg aaaacaacat tctgcaacgc ggcacgggtg gctatccgag cgttaatttc
    20941 aaaaataacg agtgcagctt ctgttacgcc tgcgcgcagg cttgccccga atcgctattt
    21001 tctccgcgcc acaccagggc ctgggatttg cagttcacca tcggggacgc gtgcctggcg
    21061 tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc aattatcttt
    21121 cgcccgacgc tctccgggat ctaccaggcg caacttaata gccaactttg caacggatgt
    21181 ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct ccatgcacac
    21241 taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt cagacatcag
    21301 cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc cgagcggtca
    21361 gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca ttgagtcagt
    21421 acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg aagagcaagg
    21481 tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc cgttgcggcc
    21541 gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag
    21601 gaagccatta aatgggataa agcgccgtgc cgtttctgcg gtactggttg cggcgttctg
    21661 gtcggaacgc agcaggggcg tgtggtggcc tgtcagggcg acccggacgc accggttaac
    21721 cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg taaagaccgt
    21781 ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg cgaatttacc
    21841 ccaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac cgccctgaaa
    21901 gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat ctgggaaggt
    21961 tatgccgcgt ccaagctgtt caaagcgggc ttccgttcga acaacatcga cccgaacgca
    22021 cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat ggatgagccg
    22081 atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg cgctaacatg
    22141 gcggagatgc acccgatcct ctggtcacgc atcactaaca gtcgtctctc taaccagaac
    22201 gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc ggataacggc
    22261 atcatcttta cgccgcaatc tgacctggtg atcctgaact acatcgccaa ctatatcatt
    22321 caaaacaatg cgataaatca ggacttcttc agcaagcacg ttaacctgcg caaaggggcg
    22381 acggacatcg gctacggttt acgtccgacc catccgctgg aaaaagcagc gaagaatccg
    22441 ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt tgctgagtat
    22501 acgctggaaa aaaccgctga aatgaccggc gtgccgaaag accagttaga acaactggcg
    22561 cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg cttcaaccag
    22621 catacccgtg gtgtgtgggc taacaacctg gtctacaacc tgcacctgct gaccggcaaa
    22681 atttcccagc cgggttgcgg tccgttctcc ctgaccgggc aaccttctgc gtgtggtact
    22741 gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt aactaacgag
    22801 aaacatcgtg atatctgtga gaagaagtgg aatatcccga gcggcaccat tccggcgaaa
    22861 atcggtcttc atgcggtggc acaagaccgt gcgctgaaag acggcaagct gaatgtttac
    22921 tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga gcgtatgccg
    22981 ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac agtcagtgcg
    23041 ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg cgcttacggt
    23101 aacgccgaac gccgtactca gttctggcgt cagcaggtac aggcgccagg cgaagcgaaa
    23161 tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga agtatggccg
    23221 gaagagctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga agttctgtat
    23281 gccacccccg aagtgagcaa attcccggta tccgaactgg cggaagatca gctgaacgat
    23341 gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta cgcatggttt
    23401 ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc gcgcggtctg
    23461 cgctggccgg tggtgaatgg taaagaaaca cagtggcgtt acagcgaagg taacgacccg
    23521 tacgtgaaag cgggcgaagg ctataagttc tacggtaaac cggatggcaa agcggtgatc
    23581 ttcgcactgc cgttcgaacc ggcggcggaa gcaccggatg aagagtacga cctgtggctc
    23641 tctaccggac gcgttctgga gcactggcac accggcagta tgactcgccg tgtgccggaa
    23701 ctgcaccgcg ccttcccgga agcggtcttg tttattcacc cgctggatgc gaaagcgcgc
    23761 gatctgcgcc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt gatctcgatt
    23821 gttgaaacgc gcggtcgtaa ccgtccgcca cagggcctgg tgtacatgcc gttcttcgac
    23881 gccgcacagc tggttaacaa actgacgctg gatgcgaccg atccgctctc gaaagagacg
    23941 gacttcaaga agtgcgcggt caaactggag aaggtgtaag ccattatgtc ccggtcagcg
    24001 aaacctcaaa atggtcgccg ccgctttctg cgcgatgtcg ttcgcacagc aggcgggctg
    24061 gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc tggcgtgcgg
    24121 ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt gcgttgcggt
    24181 cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc ctctggtctg
    24241 tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg tgaggacatt
    24301 ccgtgcgcca aagtgtgccc aagcggtgcg ctggatcgtg agattgaatc gatcgacgac
    24361 gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt tcaggggctg
    24421 cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac cttggagctg
    24481 gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca cagcgacgcc
    24541 tgtaccggtt gcggtaagtg cgaaaaagta tgcgtgctgg aacaaccggc aatcaaggtg
    24601 ttaccgctgt cactggcgaa aggggagtta ggtcaccatt accgcttcgg ctggctggag
    24661 gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga aaaaaggctg
    24721 gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg tgctggggat
    24781 gtttttgagc ggtccatggt ttggtgtgtg gatcttgcac ggcaactaca gcagtagcct
    24841 gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc ttgccagtgg
    24901 tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc tctacgccct
    24961 ggcgggaaag cgattatttt gcagctgggt ctgcccgctg aacccgatta ccgacttagc
    25021 gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc gccacattcg
    25081 ctacgtgctg ttggtggtca ttctggtggg atcagcctta actggcacgc tcatttggga
    25141 atggatcaac cccgtttctc tgatggggcg tagcctggta atgggcttcg gcagcggcgc
    25201 gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg gctggtgcgg
    25261 gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg tgattaccgt
    25321 tgcggccagc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg tttgcccgga
    25381 accgcatgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg tcaccagccg
    25441 cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat ttacaataac
    25501 tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa agcgctgtgt
    25561 caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc taatggcgtc
    25621 gattttagcc agtcgccgga agtatccggg acgcaggaag gggccattcg gatgccaaaa
    25681 gagcaggatc ggatgccgct gaactatgtg aatcagccgc caatgatccc gcatagcgtt
    25741 gaaggttatc aggtaacgac caataccaat cgctgcttgc agtgccacgg tgtcgaaagc
    25801 tatcgcacca ctggcgcgcc gcgtatcagt cctactcact ttatggacag cgacggcaaa
    25861 gtgggtgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt accgcaggcc
    25921 gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg gaaataagag
    25981 gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg gaaatggtgg
    26041 cgtaccccca gccatctggc gctggggacg ctgctgttga tcggttttgt tggcggcatc
    26101 gtcttctggg ggggctttaa caccgggatg gaaaaagcca ataccgaaga gttctgcatt
    26161 agctgccacg aaatgcgcaa cacggtgtat caggaataca tggattccgt gcactacaac
    26221 aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga gtttgtgccg
    26281 aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt tggcgttatt
    26341 gacacgccgc agaaatttga agctcatcgt ctgacgatgg cacagaatga gtggcggcgc
    26401 atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta tatggataca
    26461 accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga tgggcaaacc
    26521 tgtattgatt gccataaagg gatcgcgcac aagctgcccg atatgcgtga agtcgagcca
    26581 ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact ttgtgagcgg
    26641 gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga gtgggtacaa
    26701 atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac ggggttgtct
    26761 cgccctgacg ctggcgaggt tctctggcaa gggcagccct tgcatcaggt acgcgacagc
    26821 taccatcaaa ccctgttatg gataggccat cagccgggga tcaaaacccg gctgacggcg
    26881 ttagaaaatc tgcactttta tcatcgcgat ggcgatgccg cacaatgtct ggaagccttg
    26941 gcgcaggccg ggcttgccgg attcgaagat attccggtaa atcaactttc tgccgggcaa
    27001 caacgccgcg tcgctttagc gcgtctgtgg ttgacccgtg ccacattatg gatcctcgac
    27061 gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg tatggcgcag
    27121 catacggagc agggggggat tgtgattctg actacccacc agccgctcaa cgttgctgaa
    27181 agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc tggcgtattt
    27241 tccgtcttga actgcgcgta gcgtttcgcc atagcgccga aatcgccaac ccgctgtggt
    27301 tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg caactgctgg
    27361 cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg ctggcgctgg
    27421 aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg ttgttgccgt
    27481 tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta accggtctgc
    27541 cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt tatggctggc
    27601 aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg
    27661 gcgtggcgct gacagtggga cttaagcgcg gtggtgtgct gctcagcata ctggtgttac
    27721 cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct tctatgcatt
    27781 tgcccgttga tgggtatctg gcaattttag gcgcgttgct ggcaggcacc gcgacattaa
    27841 gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg attactgatt
    27901 tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg cgatcccacc
    27961 acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca gtgtggtcgt
    28021 gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc agcagggaaa
    28081 tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg gcatttatgc
    28141 atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg ccaacctggc
    28201 ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg ttaccggctc
    28261 tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc tgacttctga
    28321 actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct tcgacgaccg
    28381 ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga atctgccgat
    28441 tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc ggatgcagca
    28501 aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct tcctgctcct
    28561 gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg
    28621 tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccctgc atttgcttcc
    28681 tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc ggtggtgatg
    28741 accgttattc cgctggtggt tttggtcgtg cactcggtga tgcaacatcg cgcaattctg
    28801 cgtggtgtgg cgcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag
    28861 gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgccgtg ttggcagggc
    28921 tagcgctgac tatcggtctg gtgctatatg cgctgcgctc gaatatcgat ctcttttata
    28981 cgccggggga aattctctac ggcaagcgtg aaactcagca gatgccggaa gtcggtcagc
    29041 gtctgcgcgt tggcgggatg gtgatgccgg gtagcgtgca gcgcgatccc aattcgctga
    29101 aagtgacctt caccatttac gatgctgaag gctcagtgga tgtctcttac gaaggcattt
    29161 tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg gaaaaaggca
    29221 atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg ccgccagaag
    29281 ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag gacccagcat
    29341 catgatgccg gaaattggaa acgggctgct gtgcttggcg ttagggattg cgctgctgct
    29401 gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg cgtcttcccg
    29461 cttgtttgcc tggctgctgt ttatgtctgt ggctggcgca tttctggtgc tggtcaatgc
    29521 tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata cccagcttcc
    29581 ggtgtggtat cgcgtggcgg ctacttgggg cgcgcatgaa ggttcgcttc tgctgtgggt
    29641 gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta ttccgctgga
    29701 tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt tgctgttcat
    29761 tctctttacc tctaacccgt tctcacgcac gctgccgaac ttcccgattg aagggcgcga
    29821 tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc tttatatggg
    29881 gtacgtgggt ttctcggtgg cgtttgcttt tgccattgct tctttgctga gcgggcgtct
    29941 ggacagcact tatgcgcgtt ttactcgccc gtggacgctg gcggcgtgga ttttcctaac
    30001 gctcggcatt gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg gtggctggtg
    30061 gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga ctgcactgat
    30121 gcactcactg gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat tactgctggc
    30181 aatcagtgcc ttctcgttgt gtctgttggg gactttcctc gtgcgttccg gcgtgctggt
    30241 atcggtacac gcgtttgcgt ctgatccggc gcgcggtatg tttatcctcg cctttatggt
    30301 gctggtgatt ggcggttcgc tgctgctgtt tgccgcgcgt ggacacaaag ttcgctcacg
    30361 cgtaaacaat gcgctgtggt cgcgggaatc tttgctgtta gcgaacaacg ttttgctggt
    30421 cgccgcgatg ctggt
//
LOCUS       DAJTMX010000034        30296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTMX010000034 DAJTMX010000000
VERSION     DAJTMX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.034"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO145_001587"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO145_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5466741.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO145_001588"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO145_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5466742.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO145_001589"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO145_001589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5466743.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO145_001590"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO145_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO145_001591"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO145_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5466744.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO145_001592"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO145_001592"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5466745.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO145_001593"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO145_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5466746.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO145_001594"
     CDS             7489..7638
                     /locus_tag="OO145_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466747.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO145_001595"
     CDS             7839..9422
                     /locus_tag="OO145_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5466748.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO145_001596"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO145_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5466749.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO145_001597"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO145_001597"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5466750.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO145_001598"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO145_001598"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5466751.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="OO145_001599"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="OO145_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="OO145_001600"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="OO145_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="OO145_001601"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="OO145_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="OO145_001602"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="OO145_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5466752.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="OO145_001603"
     CDS             20752..22167
                     /locus_tag="OO145_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5466753.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="OO145_001604"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="OO145_001604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5466754.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="OO145_001605"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="OO145_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5466755.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="OO145_001606"
     CDS             24477..24809
                     /locus_tag="OO145_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5466756.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="OO145_001607"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="OO145_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5466757.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="OO145_001608"
     CDS             complement(26659..26976)
                     /locus_tag="OO145_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466758.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="OO145_001609"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="OO145_001609"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5466759.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="OO145_001610"
     CDS             complement(28363..29136)
                     /locus_tag="OO145_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5466760.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO145_001611"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO145_001611"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5466761.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7010 a         8049 c         7828 g         7409 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg catcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgatt
//
LOCUS       DAJTMX010000035        30064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTMX010000035 DAJTMX010000000
VERSION     DAJTMX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.035"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..501
                     /locus_tag="OO145_001612"
     CDS             <1..501
                     /locus_tag="OO145_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCR5466762.1"
                     /translation="WEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKV
                     AEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNF
                     SLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDA
                     GFARIV"
     gene            641..1300
                     /locus_tag="OO145_001613"
     CDS             641..1300
                     /locus_tag="OO145_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5466763.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(1340..2239)
                     /gene="yqhC"
                     /locus_tag="OO145_001614"
     CDS             complement(1340..2239)
                     /gene="yqhC"
                     /locus_tag="OO145_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5466764.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            2433..3596
                     /gene="yqhD"
                     /locus_tag="OO145_001615"
     CDS             2433..3596
                     /gene="yqhD"
                     /locus_tag="OO145_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5466765.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            3701..4528
                     /gene="dkgA"
                     /locus_tag="OO145_001616"
     CDS             3701..4528
                     /gene="dkgA"
                     /locus_tag="OO145_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5466766.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            4728..5654
                     /locus_tag="OO145_001617"
     CDS             4728..5654
                     /locus_tag="OO145_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5466767.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            5705..5962
                     /gene="yqhH"
                     /locus_tag="OO145_001618"
     CDS             5705..5962
                     /gene="yqhH"
                     /locus_tag="OO145_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5466768.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(6005..8224)
                     /locus_tag="OO145_001619"
     CDS             complement(6005..8224)
                     /locus_tag="OO145_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5466769.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(8335..9747)
                     /gene="ftsP"
                     /locus_tag="OO145_001620"
     CDS             complement(8335..9747)
                     /gene="ftsP"
                     /locus_tag="OO145_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5466770.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(9822..10559)
                     /gene="plsC"
                     /locus_tag="OO145_001621"
                     /gene_synonym="parF"
     CDS             complement(9822..10559)
                     /gene="plsC"
                     /locus_tag="OO145_001621"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5466771.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(10793..13051)
                     /gene="parC"
                     /locus_tag="OO145_001622"
     CDS             complement(10793..13051)
                     /gene="parC"
                     /locus_tag="OO145_001622"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5466772.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(13597..14079)
                     /locus_tag="OO145_001623"
     CDS             complement(13597..14079)
                     /locus_tag="OO145_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5466773.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(14132..14524)
                     /gene="ygiW"
                     /locus_tag="OO145_001624"
     CDS             complement(14132..14524)
                     /gene="ygiW"
                     /locus_tag="OO145_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5466774.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            14676..15335
                     /gene="qseB"
                     /locus_tag="OO145_001625"
     CDS             14676..15335
                     /gene="qseB"
                     /locus_tag="OO145_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5466775.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15332..16606
                     /gene="qseC"
                     /locus_tag="OO145_001626"
     CDS             15332..16606
                     /gene="qseC"
                     /locus_tag="OO145_001626"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5466776.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(16652..16984)
                     /locus_tag="OO145_001627"
     CDS             complement(16652..16984)
                     /locus_tag="OO145_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5466777.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            17303..17884
                     /gene="mdaB"
                     /locus_tag="OO145_001628"
     CDS             17303..17884
                     /gene="mdaB"
                     /locus_tag="OO145_001628"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5466778.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            17915..18229
                     /locus_tag="OO145_001629"
     CDS             17915..18229
                     /locus_tag="OO145_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5466779.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(18277..20169)
                     /gene="parE"
                     /locus_tag="OO145_001630"
     CDS             complement(18277..20169)
                     /gene="parE"
                     /locus_tag="OO145_001630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5466780.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(20198..20779)
                     /gene="yqiA"
                     /locus_tag="OO145_001631"
     CDS             complement(20198..20779)
                     /gene="yqiA"
                     /locus_tag="OO145_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5466781.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(20779..21606)
                     /gene="cpdA"
                     /locus_tag="OO145_001632"
     CDS             complement(20779..21606)
                     /gene="cpdA"
                     /locus_tag="OO145_001632"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5466782.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(21631..22053)
                     /locus_tag="OO145_001633"
     CDS             complement(21631..22053)
                     /locus_tag="OO145_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5466783.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(22054..22683)
                     /gene="nudF"
                     /locus_tag="OO145_001634"
     CDS             complement(22054..22683)
                     /gene="nudF"
                     /locus_tag="OO145_001634"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5466784.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     VSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            22888..24369
                     /gene="tolC"
                     /locus_tag="OO145_001635"
     CDS             22888..24369
                     /gene="tolC"
                     /locus_tag="OO145_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5466785.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            24517..25188
                     /locus_tag="OO145_001636"
     CDS             24517..25188
                     /locus_tag="OO145_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5466786.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            25194..26354
                     /locus_tag="OO145_001637"
     CDS             25194..26354
                     /locus_tag="OO145_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5466787.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(26392..27180)
                     /gene="ygiD"
                     /locus_tag="OO145_001638"
     CDS             complement(26392..27180)
                     /gene="ygiD"
                     /locus_tag="OO145_001638"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5466788.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            27323..28096
                     /gene="zupT"
                     /locus_tag="OO145_001639"
     CDS             27323..28096
                     /gene="zupT"
                     /locus_tag="OO145_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5466789.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(28154..28324)
                     /locus_tag="OO145_001640"
     CDS             complement(28154..28324)
                     /locus_tag="OO145_001640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466790.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(28585..29238)
                     /gene="ribB"
                     /locus_tag="OO145_001641"
     CDS             complement(28585..29238)
                     /gene="ribB"
                     /locus_tag="OO145_001641"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5466791.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(29342..29490)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            29612..29902
                     /gene="ubiK"
                     /locus_tag="OO145_001642"
     CDS             29612..29902
                     /gene="ubiK"
                     /locus_tag="OO145_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5466792.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         7541 a         7966 c         7457 g         7100 t
ORIGIN      
        1 tgggagcagc tacgggaaaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc
       61 tatataacca gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc
      121 agtctgaaag tggccgaatg gctggcagaa catccgcaag ttgcgcgagt taaccaccct
      181 gccctgcctg gcagtaaagg acacgaattc tggaaacgag actttacagg cagcagcggg
      241 ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat
      301 aacttcagtt tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca
      361 aatcaaccag aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc
      421 ttgattcgcc tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc
      481 ggttttgcac ggattgtata acattgccac ttttggacaa ttttgcagac attttattgt
      541 gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat
      601 aggcagataa aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat
      661 catcgctgcg ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt
      721 tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt
      781 tttgccaggc gacagcttgt tgatactggc aggcgcattg attgcccaag gggttatgga
      841 ttttctgcct acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta
      901 tattcagggg agctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc
      961 tgctaaatat caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct
     1021 ggctggacgt tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg
     1081 tctgccaaac cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt
     1141 ggtaaccagt tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca
     1201 ggtaatgacg ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac
     1261 gctgtttgtg gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc
     1321 tgctgtaccg ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc
     1381 ccggcgtcac accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc
     1441 cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct
     1501 tatgcagacg gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat
     1561 tatggtggaa cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt
     1621 tttcggtgta tttattctca attcgtttca gcacgcggct aatcagactg aagtgagtct
     1681 ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa
     1741 tttcgcggat gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga
     1801 gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg
     1861 ctgccatcga cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact
     1921 gcaaaatatc gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt
     1981 cgcactcaaa cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca
     2041 cgcgttcatt gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt
     2101 acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca
     2161 gttctgacaa actattttct ttatttttca gtttattaac tttatccgcc agcaagcgac
     2221 aaatctcttc acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg
     2281 cgcaattttg tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg
     2341 agcattgaga gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg
     2401 ccctcatatt ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa
     2461 cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg
     2521 ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag
     2581 ttctggatgc cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg
     2641 cttatgaaac gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc
     2701 tggcggttgg cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact
     2761 atccggaaaa tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg
     2821 ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg
     2881 cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt ccattctgca catgttcagc
     2941 cggtatttgc cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta
     3001 acggcgtagt ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg
     3061 ccaaaattca ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa gatggtccga
     3121 aagccctgaa agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgactc
     3181 aggcgctgaa cggtttgatt ggcactggcg taccgcagga ctgggcaacg catatgctgg
     3241 gccacgaact gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc
     3301 ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa tatgctgaac
     3361 gcgtctggaa catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa
     3421 cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg
     3481 gcagctccat cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg
     3541 aaaatcatga cattacgttg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt
     3601 tttacgcctc aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca
     3661 ccggagcctg ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat
     3721 taagctacag gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa
     3781 tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac
     3841 cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa
     3901 cagagaagaa ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga
     3961 agccctgctc gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca
     4021 ctggcccgtt cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca
     4081 aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg
     4141 cctgattgat gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat
     4201 gcaacaacgc cagctacacg cttggaacgc gacacacaaa atccagaccg aatcctggag
     4261 cccattagcg caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga
     4321 taaatacggc aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt
     4381 ggtgatcccg aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt
     4441 ccgtctcgac aaagacgaac tcggcaaaat tgcaaaactc gatcagggca agcgtctcgg
     4501 tcccgatcct gaccagttcg gcggctaacg tgcaaattct cccggtggcg gtaatgttcc
     4561 gctaccggac ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc
     4621 ttgttcagga atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa
     4681 caactcatct atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac
     4741 ttctgttttt agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg
     4801 tagaacaaac agtccggcag atttatcaga actataaatc agatgccagt acgccttatt
     4861 tcggtgaaac cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga
     4921 acgacaatct tacactaccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt
     4981 gtcaggattt tggcgatctg gtgttagaaa gcgtagcgat aacccaaact gacgtcgatc
     5041 atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacacaga
     5101 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga tgatattgtc agcaatcatg
     5161 gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt
     5221 tgcagaaaga acagccagaa tcctttgttg ctgaactctt tgaacatatt gctgattata
     5281 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggttaat gccttctata
     5341 aaaccacctt caagacggcc aataatcccg atgaagatat gcaaatagaa cggcaattta
     5401 tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc
     5461 gagtcctgga gaaaaccgcc gattccgccc gcattcatgt tcgttttacg ctgaccaatg
     5521 gcaacaatga agagcaagag ctggttttac agcggcgcga aggtaagtgg gaaatcgctg
     5581 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca
     5641 gattaaagca atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca
     5701 ctatatgaaa acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg
     5761 ctgcgtcaat gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat taaatgccaa
     5821 aatcgcccgg cttgagcagg atatgaaagc actacgccca caaatctatg ctgccaaatc
     5881 cgaagctaac agagccaata cgcgtcttga tgcacaggac tattttgatt gcctgcgctg
     5941 cttgcgtatg tacgcagaat gataaaaaaa tccccggcag catgtcagtt gccggggatt
     6001 ttttttaacg tccaaccgcc gctttagggc gtttcttcgc accagcattc accggacgag
     6061 attgcgtaga cgacgctttt tttgccgtag caggcgtctg acgctgagtc gccatcggcg
     6121 tatgtttagt caacgccgga cgggtattgc ggttctggcg acgagcttca cgcatctctt
     6181 caatggttgg cgcaggcact aagcaatcgc gacggctgcc aatcagatgc tttttgccca
     6241 tcgcttccag cgcctggcgg attaacggcc agtttgccgg atcgtggtaa cgcaacaacg
     6301 ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa gacgccttcg ctcttataac
     6361 caatcttcgc cagcggtttt ttcccggtgt aatacatggt ggttgagttc gccagcggcg
     6421 atgggtagaa gttctgtacc tggtcgagac ggaaacgatg ctttttcagc cacagcgcca
     6481 aattcaccat atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac gggatcagat
     6541 actgctcttt acctgcctgt ttcgagtagg tatcgaacag ctctttaaag cggtcatagc
     6601 tgcccatgcc cggcttcatc atcttcgaca acggcccttc ttcggtatgt tccggagcaa
     6661 tcttcagata accgccgacg tgatgggtcg ccaactcttt gatatagcgc ggatcttcca
     6721 cggcgatgtc ataacgtacg cctgaggcga taaggatttt tttaatgcct ttcagatcac
     6781 gcgcacggcg atagaggttg atcgtcggtt cgtggttagt gtccatgtgc ggacaaatat
     6841 ccggataaac gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat ttgcagcgca
     6901 acatatacat gttggcagtt ggcccaccga gatcggaaat cacgcccgta aaacctggaa
     6961 cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg ctctgaataa
     7021 tgcgcccttc gtgctcggta atagaacaga aagaacagcc gccaaagcag ccgcgcataa
     7081 tgttgaccga aaaacggatc atttcgtaag ccggaatacg ggcattgcca taggccggat
     7141 gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta gaaagcggga
     7201 tagcaggcgg gttgatccac acatagcggt caccgtgttt ttgcatcaat gcgcgggcgc
     7261 agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg ggcgtacagc actttatcgc
     7321 ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt ttcccacggt ttctggcgcg
     7381 gtggctgcac gattacagct ttcgcttcct gctttttcgg cgcaacaggt ttgttatccg
     7441 cacacggcaa atcttcgcca tacggatgcg ggattgggtc gattttccct agggtatcaa
     7501 gacgggtgga atccacaccg ctccagccag gcagcgcctc ttttacgata attgcggtat
     7561 tacgcacatc gcggatttca ctaatcggct cgcccatcgc cagacgatgc gctacttcca
     7621 ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc tttcgaatct accagaacgg
     7681 aacggcgcac ggtatcggac cagtaatcat aatgcgcggt acggcgcagg ctagcctcaa
     7741 taccgccgag gatcaccggc acatctttcc acgcctcttt acaacgctgg gtataaacca
     7801 gcgtggcgcg atccggtcgc ttacccgcga cattatctgg cgtgtaggca tcatcatgac
     7861 gtaaacggcg atcggcggta tagcggttga tcatcgaatc catgttgcca gcagtaacac
     7921 caaaaaacag attcggttta cccagacgca taaagtcgtc tttgctgctc cagtccggct
     7981 gggcgatgat cccgacgcga aagccctgcg cttccagcat acgaccgcaa atcgccatcc
     8041 cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc
     8101 agccaagttg atccatctct tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg
     8161 cggcccagta ctgcggccag gagaacaggt cgcgatccgg ttggatcagg gagatagagc
     8221 tcattttgct tccagaaatg ataaaaaaat aatcaaaggc cggggattat aagccggaac
     8281 gaaagagaaa tcgaaaggta ttccatactc gccctcctcg ggcgagtatg aagattacgg
     8341 taccggattg accaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact
     8401 gttgaagtag aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac
     8461 ttgtccgtca acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcgcc
     8521 attcacgtta cggatctgga acattacgcc ttcaatatgg aacgcttgcg gctcgtccgc
     8581 gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc
     8641 ccacagctgt ccattaatac ccgggtcatc acccagactg atatcgcgac tgcgaattgg
     8701 cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg
     8761 cagaaggccg gttgggcgca gcgttagcac caaggtagaa accagaatac tggatggctc
     8821 aaagaagccg cgaatacgat caacaatgct cgccgcttca ccacaggtga tcgacacttc
     8881 atcgccgttg ctcatatcca ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg
     8941 cttcaccgat acaggagcag gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg
     9001 accatcgctc atctgtagtt gatagcgacg agagttcgac gcgttcagca gtcgcaagcg
     9061 cacccagcca cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc
     9121 accaacaaag ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt tatccagccg
     9181 tttatcctgg ataatgaccg gaaaatcatc cacaccataa tggttgggga taggcagcga
     9241 cttgctgact tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc
     9301 cgtgcggttg ggagtattgg cgtgatacca cagagtagct gcgttctggc gaataggcag
     9361 tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcgggcc
     9421 tggtacctgt agcccggcca ccgtcattga gacattttct gtcaggcggt tgctgtaaat
     9481 aagcttaaca tcgtcgccct tccagacgcg gatagtcggc cccaggtaac gaccattgat
     9541 tccccagacc gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat
     9601 aaacagcggt tgcccacggc gagattcaag tagcggcgga acgggtagcg gttgctgttg
     9661 cccggctgcg ctggccttca ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg
     9721 aatgaactga cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac
     9781 cgtgcaggaa agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct
     9841 tcgcgttctg cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa
     9901 tgggcagcca gctcacgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc
     9961 atttcgacaa tcaccagacc gttgtgcagt cgattaagat taatcttatt cgaagttgta
    10021 gagacgcaca cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc
    10081 ttgaacggta gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa
    10141 atacggcgtt ttttgaaatg attcacgact tccgcaatgg tgccgtgagc tttagtgcga
    10201 ttgtttctgt cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc
    10261 cacagcaagc tctttttacc taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc
    10321 accatgtcat agttgttctg gtggttagcg atatagatag cattgccgta gctttcagca
    10381 tctgcaggtt tgcggcactc aactttcagg ccgaacagtg gcgcaagacg gccaaacata
    10441 tgcccaaagg tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg
    10501 aatacacaga ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata
    10561 acaccctctg aaggtctgag agactggcat tgtacgtcac ctgaaatcag gttagggcat
    10621 tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg
    10681 tttcaaggga atttttatcg tttccggggg ttgaatacgc tgccggatga cgacttaacg
    10741 tttcatccgg cgttccttgc tagcgggagg aaacagcgcc ctccccggca tattactctt
    10801 cgctatcacc gctgctggca cggcgaggag agtcgatctc aacacgatcg atacgctgca
    10861 aaccgcgcat caacgtaccg cggcgtccac gttcgccagt gactttctgt agctcttccg
    10921 gacgcagttt aattttgcgt ttcccaacat gaatggtcag cgtgctttgc ggcggcagaa
    10981 cgtacagttg cgccagacca tcctcgccac gcgcggcttc tgccgatgga atgttgataa
    11041 tcttgttgcc tttgcccttc gacagctgcg gcagatcgct taccgggaac atcaacatac
    11101 ggcctgcctg agtgattgcc agcagcatat cggaagcatc ttcaatcacc accggcggca
    11161 taacatgggc attttccggt aaggtgatca aagccttacc cgcacggtta cgcgctacca
    11221 gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc agtttctgat
    11281 cgtcgctttc catcagcata tggtcaacgg tcgccccagg cggcaacgtt aatttgccgg
    11341 tgagtggttc gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg gcatagctac
    11401 gaccggtgga atcaacaaac actaccggtt ggttactctt acctttcacc gccgctttga
    11461 agctatcacc cgctttataa ttcaggcccg gcgcgtcgat atcatggcct ttagcgctgc
    11521 gtacccagcc catctgcgac agcacaatgg tgacaggttc agacggcagc atgtcgtgct
    11581 cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga acgacgatcg tcaccgtagg
    11641 cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt cattttacgc tcggaagcca
    11701 aaatgccctg caactggtcg cgctcttttt ccagctcact ctgctcaccg cgaatcttca
    11761 tctcttccag tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc gcctgggttt
    11821 ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc atcttcgtta cgaatgatct
    11881 caatcacttc gtcgatgttg agaaacgcca ccagcaaacc ttccaggata tgcaggcgct
    11941 tgaggacttt ctccagacga tagttcagtc ggcggcgcac ggtatcgcga cggaacacca
    12001 gccattcgga gaggatttcc agcaggtttt tcaccgccgg acgaccatcc agaccgatca
    12061 tattgaggtt aatgcgatag ctcttttcca gatcggtggt agcgaagagg tggttcatca
    12121 cctgatccat atccacgcgg ttggaacgcg gcacaatcac cagacgggtc gggttctcgt
    12181 ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc atttgcgcag
    12241 caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg taatgcgctg ataaccaccg
    12301 cgccatcttc tttcttccac accgcgcgca tacgcactga accacgtccg ttctcgtaga
    12361 ttttacgaat ctcggcgcgc gaagtgataa tttccgcttc agtcggataa tccggcccct
    12421 gcacgatatc cagcagctga tcgagcgtag ttttcggctg gtcgattaat gcgattgccg
    12481 cctgagctac ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg acggcaatac
    12541 cggtggtgcc gttaagcaaa atgtttggca gacgggcagg tagcattttc ggctcctgca
    12601 aagtgccgtc gaagtttggc acccagtcag ccgtcccctg ccccagctcg ctcaatagca
    12661 gctcggaata tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac gatttcggat
    12721 cgtccggcgc gccccagttc ccctgaccat caaccagcgg ataacggtaa gagaacggct
    12781 gcgccatcag gaccatcgct tcataacagg cgctatcgcc gtgcggatgg tatttaccca
    12841 gtacgtcacc gacggtacgg gccgattttt taaatttggc gctggcattc aggcccagtt
    12901 cagacatcgc atacacaatg cggcgctgaa caggtttcag accatcacca ataaacggca
    12961 acgcacggtc catgatcacg tacatggagt agtttaagta ggcgttttcc gtaaattcat
    13021 gtagcgcaag gcgctctgcc atatcgctca ttaattctga ttcctcaact tattcgccag
    13081 ccttcaaagg ggcaatattg ccggagatac tacattatct gccgcgtcga gtcacaggag
    13141 atcagcgtga atagcgccgc cggacgtgct ccgacggcga taaaggcatc aatgtgcctt
    13201 gatatacaac tctttgctgt agacttgatc ggcatagagg gcagcacaag gttgcgccca
    13261 tgccacacca ctcggcatgg gggtccgctc cgggcgattc gagaagttga ggttatgcga
    13321 gtccagggaa gcctaaagca tcaaagtagt cgactgtaag tatacggttc agcccagttt
    13381 agctgtgtct ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc tcatggatat
    13441 ccccgagacg gcttacggcg caccggataa taaatttcgc gtatacatgg tattccttac
    13501 cggagaaatt ataagcccaa caacaatacg cctgtgacat tgttcgctca aacttatcgc
    13561 gagtgatttg gctcacactc cggtaaagat tccccatcac gccaacggca cataaacatc
    13621 cgtttccagt cgctgctctg tcaccccttc ggcaagattg gtgtagtgaa acagaatcgg
    13681 tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaataatgc cccatatcgt
    13741 gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg gcataacgtc caccggtaag
    13801 ttcaccattg ctcacaccat aacgattatc gggaatcggt tcgctaacgc tgccgcagat
    13861 atcaaagcga aacgcttccg gtgcggtggt ggcaggggcg tcccaggcga cgccaaaagt
    13921 ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa atttcgccac
    13981 actgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag ggattgatgg
    14041 gaaatcgata atgtttacat ccagtgtcag gtttgtcatt ttgcctccct gcgaaattat
    14101 tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg atctgtttga
    14161 cgtcaatttc aacagaattc cagtctttat cgacttcacc ctgaatctca accgtatctt
    14221 tcggcgtcac cgtcacaccg ttccagcgtt tgtgatcgat atcaacattg atagtaccgc
    14281 tggcatcttt aaacacgtag agatcgtcag agatgcgttc aacgatattg ccgcgcaggg
    14341 ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg ctgccgttcg
    14401 gcccctgaaa tcctccggcc tgactttgcg ttaccgatgg gccagaaaaa ccgccctgct
    14461 ctgctgccat caccggtgca ctgcacaggg ccattactgc gattactgct gcgaattttt
    14521 tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata gtccttaaca
    14581 acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca acacggttta
    14641 ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg atagaagatg
    14701 acatgctgat tggtgacggc atcaaaactg gccttagtaa aatgggtttt agtgtcgact
    14761 ggtttacaca aggtcgtcag ggaaaagagg cgctttatag cgcaccttat gatgcggtga
    14821 tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa tggcgagaga
    14881 aaggtcagcg tgagccggta ctgaacctga ccgcgcgtga tgcgttagcg gaacgtgtag
    14941 aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg atagaagtcg
    15001 ccgccaggct ggaggctctg atgcgccgaa ccaacggcca ggccagcaac gagctgcgcc
    15061 acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc gaacccttaa
    15121 cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct ggtcgggtac
    15181 tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc accagtaatg
    15241 ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt attcgtaccg
    15301 tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc ttagtctgcg
    15361 cgtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc tttccagctt
    15421 tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg ttcgacaccc aactgatgct
    15481 gtttgccaaa cggttaagta cgctcgatct caacgaaatc aacgcggcgg atcgcatggc
    15541 acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga cctttgccat
    15601 ctttacccac gacggcagaa tggtccttaa tgatggcgat aacggagaag atattcccta
    15661 tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg atccttggcg
    15721 ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggcc aggaatggga
    15781 ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt ggctggtcgc
    15841 actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg cgccgctgaa
    15901 caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac taaacgcgac
    15961 tggcgtaccc agcgaagtgc ggccactggt tgagtcgcta aatcaactgt tcgcccgcac
    16021 acatgcgatg atggttcgag aacgacgctt tacctccgat gcagctcacg aactacgtag
    16081 cccgttaaca gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg atgatccgca
    16141 ggcgcggaaa aaagcactgc tccaattaca ttccgggatc gatcgcgcta ctcgtctggt
    16201 tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg acgtcgcgga
    16261 gatcccgctt gaagatctcc tacaatcgtc ggtgatggat atttaccaca cggcgcagca
    16321 ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc atcaaacgca ccggacaacc
    16381 gctattgcta agtttgttgg tgcgaaattt gctggataac ggccccggtg tgacaccaga
    16441 ggcactggcg cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg ctaccggcag
    16501 tggacttggg ctatcgattg tccagcgaat cgccaagctg catgacatga atgttgagtt
    16561 tgggaatgcg gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc tctgtctatg
    16621 agagccgtta aaacgactct catagatttt actaatagca aaatataaac cgtccccaaa
    16681 aaagccacca accacaaacc agacaaataa cgagcgagaa tacatatagc caaaactcga
    16741 acatttcccg cataaagagt tttcttaaga taagaataat aagtggcgta agaagaaaaa
    16801 atgctgcaat cattgcttgc aatatacgat cttcgcctga aacggaaggt atactgcaaa
    16861 tattgcctcc cgactcaagc atccagtcat acttattgaa agcaaaaaaa gaatctatta
    16921 accaataagt aagcattata tatagcaatg cttcaaatat tgttttaatt ttctgcttta
    16981 acatatatca gtacgctcat aaaaatccat ttataattat caacatgaca cgttgatatt
    17041 ttcatctttt acttattcaa agccatattt tcaatcccca aaaaaaccga accaaacgtg
    17101 taaattattc cggtgcgagt aagaattatg aggaatggct atcagtattg tcattttcag
    17161 aaaatattta tcctgcatcg gtgagtcaga gctagatcag acttttgcta aattcgcaaa
    17221 agactttgca cattttgcta atttcacagt accgctctgt gacgtactat agtcgacaaa
    17281 cgtctcacct tgagattaaa aaatgagcaa catcctgatt atcaacggcg cgaaaaaatt
    17341 cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg
    17401 cgacctcggg catgatgtcc gcatcgttcg cgccgacagc gactacaatg tcaaagcgga
    17461 agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct ggtggatggg
    17521 cgcgccgtgg acagtgaaaa aatatattga tgatgtattc accgaaggtc acgggactct
    17581 gtatgccagc gatggtcgta cccgcaaaga tccgtcgaaa aaatacggtt ccggcagcct
    17641 ggtacaggac aaaaaatata tgctttctct aacctggaac gcaccaatgg aagccttcac
    17701 cgaaaaagat cagttcttcc acggcgttgg cgttgacggt gtgtatctgc cgttccataa
    17761 agcaaaccaa ttcctcggta tggaaccgct gccgacattt atcgctaatg acgtgataaa
    17821 aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg aaatttttgg
    17881 ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg cagaaatccg
    17941 tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta aaatcgttcc
    18001 aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt gcgctgctgg
    18061 cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt gggaaagcat
    18121 cgcgcacctt gaagcgcatc tgcaaacccc gcacatgaag gcgtacagcg aagccgtaaa
    18181 aggcgacgtg ctggagatga atatccgtat tctgcagcca gggatttaat cctgccttgt
    18241 ttgcccggcc atcctgaccg ggcaatgttc tttcctttaa acctcaatct ccgccatgtc
    18301 gcctttctct tgcaaccagt tgcggcgatc ttccgagcgt ttcttcgcca gcagcatatc
    18361 catcatcgcg tcagtacgct gatcgtcttc atcatcgata gtcaactgca ccagacggcg
    18421 agtgttcgga tcaagcgtgg tttcacgcaa ttgcatcggg ttcatttccc ccagaccttt
    18481 aaaacgctgg acgttcggct tgcctttctt gcgttttaat tgctcaagta cgccctcttt
    18541 ctcttcttcc gtcagcgcgt aataaacctc tttcccgaga tcaatacggt agagcggtgg
    18601 cagtgcgacg taaacgtgac cgtgtttcac caacgcgcgg aagtgtttta cgaacaaagc
    18661 gcagagcagc gtggcaatgt gcagaccatc agagtccgca tccgcgagga tacagatttt
    18721 gccataacga agctggctca gatcgtcgct gtcaggatcg ataccgatcg ctaccgaaat
    18781 atcatgcact tcctgcgagg ccagcacttc gtcggaagaa acttcccagg tgttaaggat
    18841 cttacctttc agcggcatga tcgcctgata ttcacgatcg cgcgcctgct tggcagatcc
    18901 gcctgcggag tcaccttcca cgaggaacag ttcggtacgg ttaaggccct gcgcggtaca
    18961 atcagccagt ttgccaggca gcgccgggcc gctggtcagc tttttacgca ccactttttt
    19021 agccgcacgc atacgacgct gggcgctgga aatcgccatc tctgccagca actccgccgc
    19081 ctgaacgttc tggttcagcc acaggatgaa ggcatctttc accacgcccg aaacgaatgc
    19141 cgcacactga cgcgaagaga gacgctcttt ggtctgcccg gcaaactgcg gatcctgcat
    19201 tttaaccgac agaacatagg cgcagcgatc ccagatatct tccgccgaca gcttcacgcc
    19261 gcgcggcaga atgttgcggt attcacagaa ctcacgcatc gcgtccaaca ggccctgacg
    19321 cagaccatta acatgggtac cgccctgcat cgttgggata aggttgacgt agctttcggt
    19381 cagcagttca ccgccttccg gcagccacag tagcgcccag tccacagctt cagtatcgcc
    19441 agcgaaatta ccgataaacg gtttttctgg cagcgtcggt aaaccgttta ccgcttccgc
    19501 caggtaatca ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt tgatctcatc
    19561 tttaaaggta atctcaacgc cggggcacag tactgctttg gccttcagca catgcgtcag
    19621 gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca tccggccaga agtgcacgct
    19681 ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt aaatcctgca ccttttcgcc
    19741 attttcaaag gcgatgttat aaacctgacc atcgcggcgc acgttaactt ctacgcgctt
    19801 cgacaaggcg ttaactaccg aaatccccac gccatgcagg ccgccagaga actggtaatt
    19861 tttgttagag aatttaccgc ctgcatgcag acggcaaaga atcagttcaa ccgccggtac
    19921 tccttcttcc gggtgaatat ccaccggcat cccgcgccca tcgtcaatca cttctaacga
    19981 ctggtcagca tgtaagatta catccacgcg ttttgcgtga cccgccagtg cttcatcgac
    20041 actgttatca ataacttctt gccccaaatg gttagggcga gtggtatcgg tatacatccc
    20101 cggacggcgg cgaaccggct caagcccggt gagtacctca atggcatcag cgttataagt
    20161 ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca gagatggtgc agaccaagaa
    20221 aatcgacgat cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg ccgccttcta
    20281 tgacagtctg gcggcaggaa gcatagtacg ccaccgcctg gcggtaatcc agcacttcat
    20341 ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca atctgcatga
    20401 ctttgagatc gtaaatatgg cgtgactcta gcacatattg ctgcccggtg taggggttct
    20461 cgttctgacc gagatagtcc gtcagcaatt caaacgggcg taccgccggg tttaccacca
    20521 ctgcgggcag cataaaacat tgcgacaacc aggtggcgta atatcccccc agtgacgaac
    20581 cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat ggattccagc agttctgccg
    20641 cgtcggaagg atacggcggc aactgcggaa tgatcatctc aacgtcaggg tgatgttccg
    20701 ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg aaaccgtgta
    20761 aataaagaag cgtagacatc agtagccttc tgaagcggta tcaggttgga aacgtgtgtc
    20821 cgccaggcga tgcacctcgg tggtcagcgt gccatcagca tgtaactcga gagtacgcca
    20881 gccgggcgcg atagtatcca gcgtaaagtt ggaacagtgc ggcttaaact gcacacaggt
    20941 cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga tccagctcct gatgaatatg
    21001 accgcacagc aagtatttga cgtgcggaaa cttcgccagc acggtatcca gttcgcccgc
    21061 gttacgcaga ctgtgttgat cgagccaact acaacccgca ggtagcggat gatgatgcag
    21121 cagcagcaac gtatggcgtt ctggcgcatc ggccagttta cgttccagcc actcaagctg
    21181 aaactcgctc agctcaccgt gcggcacgcc aaacacctgg ctatccagca acaggatttg
    21241 ccattgctca ccaataaaca cccgcttcgc cggggagata cccgcatcct gtaacgcgct
    21301 gtacatcgcg ggctggaaat cgtggttgcc cggcagccag acgcagggcg cacgaaaact
    21361 tgcgatgcct tcagcgaaat gctgataggc cgcagaggat tgatcctgcg ctaaatcacc
    21421 tgtcgcgaca atcaggtcga attcgtgctg gtgtggacga atcgcctcca gcaccgcctg
    21481 gtagctttcc caggtgttta cccctaacag ggcttcgtgc ttttgtgcaa acaggtgagt
    21541 atcggtaatt tgtaaaatcc ttcctctggc ctcaccagcc agaggaaggg ttaacaggct
    21601 ttccaaatgg tgtccttagg tttcacgacg ctagtaaacc ggaatcgcca tcgctccatg
    21661 tgctaaacag tatcgcaacc agtccgctaa aaactggtta atttgatgct tttcgtcgcg
    21721 ttgatgcaac tttttattag gataatcata ccgcgctttg aagcgaaaaa tctgctggct
    21781 tgaacacact tcagccacca tcgcgtcatg atacagacgc accgtcattg acggaaggct
    21841 ccagtaactg atcgcgggcg cagtctgttc tattgtcacc agggtagtgt atcgggtcga
    21901 ttccacaatc gtcagccgat attgtgcgtt tgccacctga tagcttacag tttcgccggg
    21961 tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc acaggcgcat
    22021 catttcagga aagtcaggtg tgtaacgctt catttatgcc cactcatttt ttaacgcttg
    22081 atgatgcagc tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga ttttcccctc
    22141 ttctacccac tggtatgcct gttcccggct taccacatga acgcgaatat cttcgttttc
    22201 atcagccaga ccatgaatac cgcttgcggt cgtggcgtcc acttcgccca ccataattga
    22261 cgaacgctca ctggtgcccc ccgggcttgc caggaaactt aacaccggtt tggtccgttt
    22321 gactatcagt cccgcctctt caatcgcttc gcgacgggca acatcttcca cactttcacc
    22381 ctcttcaatc atcccggcaa ccatctccag tagccagggg gtttcgctgg tgtcgtacgc
    22441 ggcaatccga atctgctcaa tcagcacaac ttcatcacgc actgggtcaa agggtagcaa
    22501 gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc tcatgactca cttgcccgtt
    22561 gaatagacga tgacgaaatc tataaagatc taatgaaaaa aagccgcgat aaagtgtttc
    22621 tcgtgcaata atttctacat cgtttttgcc aaatgtaacg ggcaggttgt ctggcttaag
    22681 cattgttaat gtcctagcac taatagtgaa ttaaatgtga atttcagcga cgtttgactg
    22741 ccgtttgagc agtcatatgt taaattgagg cacattaacg ccctatggca cgtaacgcca
    22801 accttttgcg gtagcggctt ctgctagaat ccgcaataat tttacagttt gatcgcgcta
    22861 aatactgctt caccacaagg aatgcaaatg aagaaattgc tccccattct tatcggcctg
    22921 agcctttctg ggttcagttc gttgagccag gccgagaacc tgatgcaagt ttatcagcaa
    22981 gcacgcctta gtaacccgga attgcgtaag tctgccgccg atcgtgatgc tgcctttgaa
    23041 aaaattaatg aagcgcgcag tccattactg ccacagctag gtttaggtgc agattacacc
    23101 tatagcaacg gctaccgcga cgcgaacggc atcaactcga acgcgaccag tgcgtccctg
    23161 cagttaactc aatccatttt tgatatgtcg aaatggcgtg cgttaacgct gcaggaaaaa
    23221 gcagcaggga ttcaggacgt cacatatcag accgatcagc aaaccttgat cctcaacacc
    23281 gcgaccgctt atttcaacgt gttgaatgct attgacgttc tttcctatac acaggcacaa
    23341 aaagaagcga tctaccgtca attagatcaa accacccaac gttttaacgt gggcctggta
    23401 gcgatcaccg acgtgcagaa cgcccgcgcg cagtacgata ccgtgctggc gaacgaagtg
    23461 accgcacgta ataaccttga taacgcggta gagcagctgc gccagatcac cggtaactac
    23521 tatccggaac tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc acagccggtt
    23581 aacgcgctgc tgaaagaagc cgaaaaacgc aacctgtcgc tgttacaggc acgcttgagc
    23641 caggacctgg agcgcgagca aattcgccag gcgcaggatg gtcacttacc gactctggat
    23701 ttaacggctt ctaccgggat ttctgacacc tcttatagcg gttcgaaaac ccgtggtgcc
    23761 gctggtaccc agtatgacga tagcaatatg ggccagaaca aagttggcct gagcttctcg
    23821 ctgccgattt atcagggcgg aatggttaac tcgcaggtga aacaggcaca gtacaacttt
    23881 gttggtgcca gtgagcaact ggaaagcgca catcgtagcg tcgtgcaaac cgtacgttcc
    23941 tccttcaaca acattaatgc ttctatcagt agtattaacg cctacaaaca agccgtagtt
    24001 tccgcgcaaa gctcattaga cgcgatggaa gcgggctact cggtcggtac gcgtaccatt
    24061 gttgatgtgt tggatgcgac caccacgctg tacaacgcca agcaagagct ggcgaatgcg
    24121 cgttataact acctgattaa tcagctgaat attaagtcag ccctgggtac gttgaacgag
    24181 caggatttgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa tccggaaaac
    24241 gttgccccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc tgatagcccg
    24301 gcacccgtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca taaccctttc
    24361 cgtaactgat gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa cgtaaagata
    24421 cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc atcctctatt
    24481 ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa atccatacgc
    24541 cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga caccagtcgc tctcgcggtt
    24601 gccactgttt ttatgctggc tggctgtgaa aagagtgatg aaacagtgtc tctctatcaa
    24661 aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac caccgcgtac
    24721 aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat acgccacccg tgaagactgt
    24781 gttgctgaat ttggtgaagg tcagtgtcag caagcaccag cccaggctgg catggcacca
    24841 gaaaaccagg cgcaggctca gcaatccagc gggagtttct ggatgccgct gatggccggt
    24901 tacatgatgg ggcgtctgat gggcggcggc gcgggatttg cacagcagcc gctgttctcc
    24961 tcgaaaaacc cggccagtcc ggcttacggt aaatataccg acgcgacggg taaaaactat
    25021 ggcgcagccc agccaggccg caccatgacc gtaccgaaga cggcaatggc accaaaaccg
    25081 gcgaccacca ctaccgttac ccgtggcggt tttggtgaat ctgttgccaa acaaagcact
    25141 atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg gtggctgata ccgatggaaa
    25201 gagtcagtat taccgagcgc ccggactggc gtgagaaagc ccacgaatac ggtttcaatt
    25261 ttcacaccat gtacggcgag ccgtactggt gtgaagatgc ttactacaag ttgaccctcg
    25321 cccaggttga aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc ctgaaagtgg
    25381 tggaaaaagt gatcgccagc gatgagctga tgaccaaatt ccgcatacca aaacacacct
    25441 ggagttttgt gcgtcaatca tggctgactc accagccatc gctttattcg cgtcttgatc
    25501 tggcgtggga tggcactggc gaacctaaac ttctggaaaa taacgccgat acgccaacgt
    25561 cactatacga ggcggcgttc tttcagtgga tctggctgga agatcagctt aacgccggta
    25621 acttgccgga gggcagcgac cagtttaaca gtctgcaaga aaaactgatc gatcgcttcg
    25681 ttgagctgcg tgaacagtat ggcttccagt tgctgcatct cacctgctgt cgcgacacgg
    25741 tggaagatcg cggaaccatt cagtatttgc aggactgcgc aaccgaagct gaaatcgcca
    25801 ccgagttcct ctacatcgac gagataggtt taggcgaaaa aggccagttc acggatttac
    25861 aggatcaggt gatttctaac ctgttcaagc tttatccgtg ggaatttatg ctgcgcgaaa
    25921 tgttctccac caagctggaa gatgctggcg tacgctggct ggaaccggcg tggaagagca
    25981 ttatctccaa caaggcactt ctaccgctac tgtgggagat gttcccgaat cacccgaacc
    26041 tgctgcccgc ttattttgcg gaagatgatc atccgcaaat ggaaaaatat gtggttaaac
    26101 cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa accattgaag
    26161 cagcggaagg tccgtatggc gaagaaggga tgattgttca gcaattccac ccgttaccga
    26221 aattcggcga cagctatatg ctgattggta gctggctggt gaacgatcaa cctgccggaa
    26281 ttggcattcg tgaagaccgt gcgttgatca cccaggatat gtctcggttt tatccacata
    26341 tttttgttga ataagccacg ataccggatg gcactcgcca tccggtaatt gttagcctat
    26401 ctgcaccgac agcatactta aactgcccat ttctatacca tcgaccggaa tggtaattgg
    26461 ctcctgccca tcccacacac ctaacacata caacaacggc agatagtgct ctggcgttgg
    26521 gttcgataac gcgccacctt catggtcgag ataattcacc agaggatgct gctctaccgg
    26581 cccttgccac gtcagattcg ctttcacata ctcattaaac gacgtcgccc acggatacgg
    26641 tgaactatca ccgtgccact tcactgtgcg caggttatgc accacgttac cgctggcgac
    26701 caacattatt ccttcatcac gcagcgccgc gagtttgcgc cccatttcga aatgccaggc
    26761 ggcaggtttg ctactgtcga tactcaactg caccataggg atatcggcgt caggatacat
    26821 cttaatcagc acgccccacg agccgtggtc aaagccccag gcttctttat ccagcgtcac
    26881 cgggatcggc gctaacagct caaccagacg ctgtgccagc gcaggcgaac ccggagcagg
    26941 ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcgt
    27001 ctccatcgcg gtcactcctg ttccacgggt aaaccagtga gccgaaacca ccacaatcgc
    27061 ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg cgggtataca aattatcttc
    27121 cagcacgttc atcggactac cgtgacctaa aaacaatgct ggcatacgtg ttgaagacat
    27181 gatgatatcc ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga
    27241 gtaagaaccc ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt
    27301 gaagatttat aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc
    27361 agccacgttt attggcgcgt ttctcggcgt tctcggacaa aaaccctcga accgcttact
    27421 ggcgttttcg ctaggttttg cggcggggat catgttgctc atctcattaa tggaaatgct
    27481 tcctgccgca ctagcagctg aaggaatgtc gccggtgttg ggttatggaa tgtttatctt
    27541 cggtctgctt ggctactttg gcctggaccg catgttgccg catgcgcatc cgcaggattt
    27601 aatgcaaaaa tcggtgcagc tgttgccaaa atcgatcaag cgcacagcca ttctgctcac
    27661 tctcggcatc agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag
    27721 caacctggag ctgggatttg gcatcgcact ggccgtcgcg ttgcacaata tccctgaagg
    27781 tctggcagtg gcaggcccgg tttatgcggc aacggggtct aaacgcaccg cgattctgtg
    27841 ggcggggatt tctggactag cagaaattct tggtggtgtg ctggcgtggt taatcctcgg
    27901 tagcatgatt tccccggtgg tcatggcggc aatcatggcg gcggttgcag gaattatggt
    27961 ggcgctctcg gttgatgaat taatgccgct cgccaaagag attgacccta ataataaccc
    28021 cagctatggc gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac
    28081 ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc acatggacaa cttctgccag
    28141 cttcacgaaa gccttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt
    28201 tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa taacgtttca
    28261 aatgcaggaa ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa
    28321 acatcctttt acccttaata tttaacaata ctaaacattt agcgcataaa tttcacatat
    28381 cctttttcgg atatattcat atggtcgcat aataacggaa caattatatg attttgatca
    28441 taatgaaaaa gaatgcctga tattaatgaa attttctcat agaaagagaa gcggaattaa
    28501 catcttgcat agcagggcaa aataaaaacc gaagccagct acaacttcgg tttcttgatt
    28561 aaggcagtaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct gacggtatgc
    28621 caccaggtct tcaatagtca cgagcgccat attgtgttta ttggcaaact caatgcactc
    28681 tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac cagccggttt
    28741 aaagcctgcc agcgtcatca aatcaatagt cgcttcagta tgaccgccac gcgtcagtac
    28801 accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat cagaaggttt
    28861 tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag aaacaccggt
    28921 agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt
    28981 attttctacc atcattggca gatcaagttg tttacggcga tcttcagtga tgcacaggca
    29041 aacaataccg ctaccgtggc gaatggtcag cgccatctgc tcaacggtca tggtttctgc
    29101 cgggaagatc atatcgcctt cgttttcacg gtcttcatca tcaagcacca ttacaccgcg
    29161 tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac caaaagagga
    29221 aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat tatggttacc agaatcaggg
    29281 cagtcttagg agtggcggca tatagccaaa ataacgtgag cgggtccatg cccgacagaa
    29341 tcgttactct ctcccatccg gactctaacc gtcggccccg gaattacacc ggatctgctg
    29401 tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac tgagttgatt taccgccggt
    29461 ggggaatttc gccccgccct gagaataagc ggattcacta taacgctaat gattagcggc
    29521 agcaacgcat agcttcacat aattctggtt tatgacttac ccttatcgca ctacaatggt
    29581 actcaacacc tatcagtaca gggaaactac aatgattgac ccgaaaaaaa ttgagcaaat
    29641 cgctcgccag gttcacgaat caatgcctaa aggaatcagg gagttcgggg aagatgtgga
    29701 gaaaaaaatc cgccaaaccc tacaagctca gctgacgcgc ctcgaccttg taagccgtga
    29761 agagttcgac gtccaaacgc aagtcctgtt acggactcgt gagaaactgg cgctgcttga
    29821 acaaagaatc agcgaactgg aaaatcgcag tactgagatc aaaaagcagc cagatccaga
    29881 gactctcccc ccaacgctgt aaacatttgt ctttaatgca ctgtaaatta tgaattaatc
    29941 ctctaattta cagtgttttc tattacatta tcgcttaatc ccattcagag tatcatcaca
    30001 aaaatgaatg attgcaattg aaaatataat tgttatgtat tctttaagtt ttacttaatc
    30061 gata
//
LOCUS       DAJTMX010000036        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTMX010000036 DAJTMX010000000
VERSION     DAJTMX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.036"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO145_001643"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO145_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5466793.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO145_001644"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO145_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5466794.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO145_001645"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO145_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5466795.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO145_001646"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO145_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5466796.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO145_001647"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO145_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5466797.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO145_001648"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO145_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5466798.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO145_001649"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO145_001649"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5466799.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO145_001650"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO145_001650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5466800.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO145_001651"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO145_001651"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5466801.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO145_001652"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO145_001652"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5466802.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO145_001653"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO145_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5466803.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO145_001654"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO145_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5466804.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO145_001655"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO145_001655"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5466805.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO145_001656"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO145_001656"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5466806.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO145_001657"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO145_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5466807.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO145_001658"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO145_001658"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5466808.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO145_001659"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO145_001659"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5466809.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO145_001660"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO145_001660"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5466810.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO145_001661"
     CDS             21622..22212
                     /locus_tag="OO145_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5466811.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO145_001662"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO145_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5466812.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO145_001663"
     CDS             22684..23379
                     /locus_tag="OO145_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5466813.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO145_001664"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO145_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5466814.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO145_001665"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO145_001665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5466815.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO145_001666"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO145_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5466816.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO145_001667"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO145_001667"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5466817.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO145_001668"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO145_001668"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5466818.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7823 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTMX010000037        29467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTMX010000037 DAJTMX010000000
VERSION     DAJTMX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.037"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            71..352
                     /locus_tag="OO145_001669"
     CDS             71..352
                     /locus_tag="OO145_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5466819.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="OO145_001670"
                     /pseudo
     CDS             318..1395
                     /locus_tag="OO145_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1788..2129)
                     /locus_tag="OO145_001671"
     CDS             complement(1788..2129)
                     /locus_tag="OO145_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5466820.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="OO145_001672"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="OO145_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="OO145_001673"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="OO145_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5466821.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /gene="holE"
                     /locus_tag="OO145_001674"
     CDS             3531..3761
                     /gene="holE"
                     /locus_tag="OO145_001674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5466822.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /locus_tag="OO145_001675"
                     /pseudo
     CDS             3863..4519
                     /locus_tag="OO145_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="OO145_001676"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="OO145_001676"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5466823.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="OO145_001677"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="OO145_001677"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5466824.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="OO145_001678"
     CDS             complement(7472..8131)
                     /locus_tag="OO145_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5466825.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /locus_tag="OO145_001679"
     CDS             complement(8458..8814)
                     /locus_tag="OO145_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5466826.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO145_001680"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO145_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="OO145_001681"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="OO145_001681"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5466827.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="OO145_001682"
                     /gene_synonym="eda"
     CDS             complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="OO145_001682"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5466828.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11217..13028)
                     /gene="edd"
                     /locus_tag="OO145_001683"
     CDS             complement(11217..13028)
                     /gene="edd"
                     /locus_tag="OO145_001683"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5466829.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="OO145_001684"
     CDS             complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="OO145_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5466830.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15076..15945
                     /gene="hexR"
                     /locus_tag="OO145_001685"
                     /gene_synonym="yebK"
     CDS             15076..15945
                     /gene="hexR"
                     /locus_tag="OO145_001685"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5466831.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16073..17515
                     /gene="pyk"
                     /locus_tag="OO145_001686"
     CDS             16073..17515
                     /gene="pyk"
                     /locus_tag="OO145_001686"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5466832.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="OO145_001687"
                     /gene_synonym="msbB"
     CDS             complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="OO145_001687"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5466833.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="OO145_001688"
     CDS             complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="OO145_001688"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5466834.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="OO145_001689"
     CDS             complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="OO145_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5466835.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21086..21841
                     /gene="znuC"
                     /locus_tag="OO145_001690"
     CDS             21086..21841
                     /gene="znuC"
                     /locus_tag="OO145_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5466836.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21838..22623
                     /gene="znuB"
                     /locus_tag="OO145_001691"
     CDS             21838..22623
                     /gene="znuB"
                     /locus_tag="OO145_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5466837.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="OO145_001692"
     CDS             complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="OO145_001692"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5466838.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="OO145_001693"
     CDS             complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="OO145_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5466839.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="OO145_001694"
     CDS             complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="OO145_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5466840.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..25276
                     /locus_tag="OO145_001695"
     CDS             24674..25276
                     /locus_tag="OO145_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5466841.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="OO145_001696"
     CDS             complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="OO145_001696"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5466842.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25834..26574)
                     /locus_tag="OO145_001697"
     CDS             complement(25834..26574)
                     /locus_tag="OO145_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5466843.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="OO145_001698"
     CDS             complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="OO145_001698"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5466844.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="OO145_001699"
     CDS             complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="OO145_001699"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5466845.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29255..>29467
                     /locus_tag="OO145_001700"
     CDS             29255..>29467
                     /locus_tag="OO145_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5466846.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7227 a         7568 c         7235 g         7437 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tattttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctag cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgctgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tgtttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgagatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt catctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc
    10501 tacaaaaatg cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca
    10561 gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga
    10621 accagccagg aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta
    10681 gccggagaaa taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc
    10741 tgcagggctt tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg
    10801 tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc
    10861 ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca
    10921 cccgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc
    10981 gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga
    11041 gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt
    11101 tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca
    11161 cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa
    11221 aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat
    11281 aattcacgtc ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca
    11341 gccagttccg cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg
    11401 atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg
    11461 atagctgacg gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt
    11521 ttgaaacacc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt
    11581 ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc
    11641 gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc
    11701 acctggttct caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt
    11761 aacacttttg tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg
    11821 tcgagtgatt tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca
    11881 agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct
    11941 gctttgagca gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata
    12001 tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag
    12061 aagtcatccc agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg
    12121 tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca
    12181 tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga
    12241 cgcgcagcgg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag
    12301 cctggcaact gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag
    12361 aaagtacatg ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg
    12421 tccactttac cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg
    12481 cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc
    12541 atcgtcagac ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg
    12601 ttatgggaca gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat
    12661 tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc
    12721 tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa
    12781 ggctggtgcg cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac
    12841 atgcttttca aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta
    12901 ccgcatgcca actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga
    12961 taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt
    13021 ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca
    13081 ttcagaggat ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag
    13141 tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt
    13201 gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga
    13261 tattactcaa actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg
    13321 ggtccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca
    13381 gtaatggagt ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc
    13441 tgaataccac gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga
    13501 ttaaaggttt ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca
    13561 aggccaggaa ctttattcag tacctggata tccacgcctt catcaggttg cagacggata
    13621 gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt
    13681 ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag
    13741 aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct
    13801 gtattgctgc ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg
    13861 gcgaagcccg cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg
    13921 cggcgcaggg acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca
    13981 gatggcggag acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca
    14041 cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc
    14101 acggtaatct caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta
    14161 gcaaaacgca gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg
    14221 taaacctggc actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc
    14281 agcgacgtcc ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc
    14341 tcgccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata
    14401 gtgatacgat ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg
    14461 acatcgagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg
    14521 gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc
    14581 cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc
    14641 agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg
    14701 accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta
    14761 acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta
    14821 cgagcttgcg aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa
    14881 gcgcggcaaa actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc
    14941 atgttatgaa aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg
    15001 cgtaatatct gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt
    15061 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
    15121 aaatcagagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
    15181 agtattgctg ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
    15241 cgcagcatgg acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg
    15301 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg
    15361 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
    15421 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
    15481 ttaggctctt ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
    15541 ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac
    15601 ggagacgtgg tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg
    15661 cagctggcac gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc
    15721 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
    15781 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
    15841 ttgcgacgcg gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
    15901 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg
    15961 tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat
    16021 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa
    16081 aaggcttcgc agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa
    16141 tcttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc
    16201 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
    16261 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
    16321 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
    16381 tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc
    16441 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg
    16501 catgaaagtg ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa
    16561 caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa
    16621 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga
    16681 tctgaactat gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
    16741 ggttgaacgt gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc
    16801 tgacgtggta atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt
    16861 cggcattcag aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc
    16921 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
    16981 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc
    17041 tgggcagtat ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa
    17101 aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga
    17161 agctattgcc atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat
    17221 taccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc
    17281 aattttcgcc atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt
    17341 tacgccggtg ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa
    17401 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga
    17461 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
    17521 ttgccggatg cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt
    17581 tcgaatattc tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg
    17641 cttttttatt tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg
    17701 cgagttttca gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc
    17761 tcgacttctt cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc
    17821 atcggtgggc gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc
    17881 ggtacaacgc gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta
    17941 taggtggcaa agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa
    18001 taatatcccc agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc
    18061 gcatgcagac gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg
    18121 ttgccctgat tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca
    18181 ggaatatcga cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc
    18241 atctcttcga tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg
    18301 cgtattgcca actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca
    18361 acaatcgctt cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac
    18421 gcacgacggc gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg
    18481 ggatcacgga actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat
    18541 gctccccagt agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta
    18601 ttttttttcg tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag
    18661 tgtagcggca caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc
    18721 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca
    18781 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
    18841 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc
    18901 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca
    18961 gaatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
    19021 aataacctgc tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca
    19081 ctggcgtacc ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca
    19141 ccggattagt tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga
    19201 atcgcaagaa tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag
    19261 cgcggattgc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
    19321 gctcacgttt accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt
    19381 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
    19441 tggttaaacc ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca
    19501 gattgttaac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
    19561 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
    19621 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
    19681 cggcagcagc aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
    19741 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
    19801 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
    19861 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
    19921 gcgtggcatc acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
    19981 tgagcgaccc caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
    20041 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
    20101 actggttggc taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg
    20161 tccccagggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
    20221 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
    20281 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
    20341 caagcggtgt cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat
    20401 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt
    20461 gtgcctcaaa atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca
    20521 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
    20581 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat
    20641 catcatcgcc gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg
    20701 ttacctgctt cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg
    20761 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
    20821 aataatcatg ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag
    20881 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
    20941 cctgtgttgc acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat
    21001 gtaacataat gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca
    21061 tgactactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
    21121 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat
    21181 tttgacttta cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg
    21241 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
    21301 accgcagaag ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt
    21361 acgccctggt acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
    21421 cctgattaac gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc
    21481 gcgagcattg ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga
    21541 tgtaaatggc caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg
    21601 tggcgtttta atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct
    21661 gtgcctgaat caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
    21721 gtttatttca atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
    21781 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
    21841 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
    21901 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
    21961 tcattacttg gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
    22021 gccgttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
    22081 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt
    22141 agtctgatgt ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca
    22201 gtgacgccag aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg
    22261 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
    22321 ggtgtgaaat tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
    22381 gtagcgatga aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct
    22441 actgcgcgtc gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg
    22501 atggtggcag taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg
    22561 tcggtggtgc tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc
    22621 taatctatcg ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
    22681 cgctatctct ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg
    22741 tcggatgcgg cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag
    22801 tgattccacg cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc
    22861 tgaatcaaat aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc
    22921 gccaggttat ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat
    22981 ttgcggtcca tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca
    23041 gcgatatctg ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc
    23101 agcagacggt tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg
    23161 tcatcactca tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc
    23221 ggcacctgat aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc
    23281 agcgaacctg cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg
    23341 gagcgtgccg ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg
    23401 tacagcactt cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca
    23461 tgcggttcaa ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca
    23521 gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta
    23581 cccaaccccg gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct
    23641 gctttgatga aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt
    23701 aatttagggc gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc
    23761 agacggtctg cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta
    23821 atgtttcact gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg
    23881 gtttatagcc tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg
    23941 ggctagcagg agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac
    24001 ctttaaatcg gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg
    24061 gcagtttcac cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg
    24121 acattccgga gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact
    24181 ctttgaacaa cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac
    24241 gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac
    24301 aggtcatcgg catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg
    24361 gttgtttttc aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga
    24421 atcaaagata atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga
    24481 aaacttgcga ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt
    24541 atattggaag caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat
    24601 acattgcgac tcctgaaaaa taattttttt atgctattac gtatatttgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    24901 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    24961 ggttccctcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca
    25021 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    25081 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    25141 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    25201 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact
    25261 gaccgaggcg gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc
    25321 atctgcatcg cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg
    25381 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    25441 cttttttcgg cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    25501 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    25561 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    25621 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    25681 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg
    25741 cggatgacgc cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc
    25801 acgcgtctcc gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga
    25861 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    25921 acgcatcagt ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    25981 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    26041 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc
    26101 acctgcttcc agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac
    26161 gccttttttg ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt
    26221 gctgaatgcg tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    26281 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    26341 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    26401 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    26461 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    26521 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    26581 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    26641 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    26701 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    26761 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    26821 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt
    26881 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    26941 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    27001 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc
    27061 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    27121 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    27181 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    27241 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    27301 ggatattgtc ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    27361 cgtgcggcgg agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt
    27421 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    27481 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    27541 cattttccgg tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat
    27601 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    27661 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca
    27721 gtgcgcccat cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    27781 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
    27841 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    27901 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    27961 gcgatgcgcc acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgctggcc
    28021 ccgcaaatac tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg
    28081 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    28141 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    28201 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    28261 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    28321 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    28381 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    28441 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    28501 gcaccaggct ggtgatttta gcgcgggttt tcaggcgttg cgccatttcc gggcgacgca
    28561 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    28621 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
    28681 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
    28741 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    28801 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    28861 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    28921 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    28981 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    29041 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    29101 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
    29161 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    29221 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
    29281 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    29341 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    29401 gcccgtgttt ctggtgcgag ttggctggtc tgccgattac gccgaagcat taaaacagcc
    29461 ggttgat
//
LOCUS       DAJTMX010000038        29115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTMX010000038 DAJTMX010000000
VERSION     DAJTMX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.038"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..806)
                     /locus_tag="OO145_001701"
     CDS             complement(<1..806)
                     /locus_tag="OO145_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5466847.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFRETILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGKPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWV"
     gene            complement(698..1030)
                     /locus_tag="OO145_001702"
                     /pseudo
     CDS             complement(698..1030)
                     /locus_tag="OO145_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1130..1474)
                     /locus_tag="OO145_001703"
     CDS             complement(1130..1474)
                     /locus_tag="OO145_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466848.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFGKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(1576..1755)
                     /locus_tag="OO145_001704"
     CDS             complement(1576..1755)
                     /locus_tag="OO145_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HCR5466849.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKESEGNEKS"
     gene            complement(1852..2511)
                     /locus_tag="OO145_001705"
     CDS             complement(1852..2511)
                     /locus_tag="OO145_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5466850.1"
                     /translation="MSTITREWLQQAINDYESVRDELPFGLDDYQGNILAALRIALAS
                     LEAEPIGAFHIAEQQVDGTSDYIKDGEWPIDNGIIEVYAAPPVPVVPAALPENDDEDG
                     HDIDYLEPSEVYALGRTAGWNACRAAMLQGKSEQPQNAQQNIPENIPGGNSPVTPDGW
                     ISCSDRMPNEEDVLVYCSDTKEQMVGFHKGKGLFQFFYMNGVEGVCEPSHWMPLPEPP
                     L"
     gene            complement(2508..3242)
                     /locus_tag="OO145_001706"
     CDS             complement(2508..3242)
                     /locus_tag="OO145_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5466851.1"
                     /translation="MSKIDYQALRAKAEKATCGEWSLEYGEGRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQIEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELGGKDSKIANLTAERDALREGEMGDARHSNTRAAADIYFQLVEE
                     CEIPAGGSLVEYVDDMREKLEAAEKRIAELELREVVLPQCYSMLHRVDFDEPYHTEMV
                     YRQHQVLEALHNAGINVTEACKGEAS"
     gene            complement(3229..3462)
                     /locus_tag="OO145_001707"
     CDS             complement(3229..3462)
                     /locus_tag="OO145_001707"
                     /inference="COORDINATES: protein motif:HMM:NF025712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HCR5466852.1"
                     /translation="MSNLLPCPFCGGAAHVASEADHPEYGSGGRFYFVRCGTCRAQSG
                     SKYAAPGNDCAIFYSEVRAEWNQRAKEANSEQD"
     gene            complement(3459..4133)
                     /locus_tag="OO145_001708"
     CDS             complement(3459..4133)
                     /locus_tag="OO145_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466853.1"
                     /translation="MKQMTLIEMDGFLKGKCIPRDLNVNETNAEYLVRKFGELESKLE
                     TALRECRSAGITIDNLDAKCAALAAESAVMKAGHSYFSYGSEHNFEWHKTAEEAIESA
                     EAAIDDYRGDACDGWSEEVDSICWGIIMQSSTKVGERPRNEDDCCDPAIDTICDYALL
                     PNIETPATDAFLAEVRAKAFDDLCAAFVKHASVSGLDDGDCVTVKEATDALLHCAEQL
                     HKGVHS"
     gene            complement(4130..4294)
                     /locus_tag="OO145_001709"
     CDS             complement(4130..4294)
                     /locus_tag="OO145_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR5466854.1"
                     /translation="MRGLAYNPGILPAEMIIRQRLKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            complement(4305..4601)
                     /locus_tag="OO145_001710"
     CDS             complement(4305..4601)
                     /locus_tag="OO145_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR5466855.1"
                     /translation="MPAPLYGADAPRRCSGNSVLEVLDKFRKNYDRIMSLPQETKEKK
                     EIRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEYIDPRLRNYRSRYGAISND"
     gene            complement(4618..5166)
                     /locus_tag="OO145_001711"
     CDS             complement(4618..5166)
                     /locus_tag="OO145_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5466856.1"
                     /translation="MNHLMVDLETMGNGPYAPVISIGAVFFDPNTGETGEEFSVNISL
                     ESSMRYRARPDASTILWWMEQSEEARKSLTSNTQELSTALSWLSEFIIKNANHKFVQV
                     WGNGASFDCVILRNSYSLTGQPVPWQWWNDRDVRTIVELGKAIGFDPKRDMPFKGTRH
                     NALDDAIHQAKYVSAIWKKLAK"
     gene            complement(5175..5681)
                     /gene="ssb"
                     /locus_tag="OO145_001712"
     CDS             complement(5175..5681)
                     /gene="ssb"
                     /locus_tag="OO145_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5466857.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(5682..6389)
                     /locus_tag="OO145_001713"
     CDS             complement(5682..6389)
                     /locus_tag="OO145_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5466858.1"
                     /translation="MNLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQHDWQLMAGNEDAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(6644..6796)
                     /gene="kil"
                     /locus_tag="OO145_001714"
     CDS             complement(6644..6796)
                     /gene="kil"
                     /locus_tag="OO145_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR5466859.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(6781..6912)
                     /locus_tag="OO145_001715"
     CDS             complement(6781..6912)
                     /locus_tag="OO145_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR5466860.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(6937..7905)
                     /locus_tag="OO145_001716"
     CDS             complement(6937..7905)
                     /locus_tag="OO145_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR5466861.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKSKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEEAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRINDEEERRA
                     KDKAHRKEVNNKILADLIKVGASENVAKNIITAIVKGEVFATKITY"
     gene            complement(8092..8541)
                     /locus_tag="OO145_001717"
     CDS             complement(8092..8541)
                     /locus_tag="OO145_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466862.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLIAILTIPLVFWFMKKQCQRVETGKIGDEGYSWERGNPKYRPTMVWDSKGDI
                     NLLIMPLVGVLALWGIFIIGAVTNMTWLKIWLAPKLYLIEYAASLVK"
     gene            complement(8550..8876)
                     /locus_tag="OO145_001718"
     CDS             complement(8550..8876)
                     /locus_tag="OO145_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCR5466863.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLTRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(9241..9645)
                     /locus_tag="OO145_001719"
                     /pseudo
     CDS             complement(9241..9645)
                     /locus_tag="OO145_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9642..10274)
                     /locus_tag="OO145_001720"
     CDS             complement(9642..10274)
                     /locus_tag="OO145_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5466864.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPLTPINGNCKIIGVVVEARVKFV"
     gene            10378..10593
                     /locus_tag="OO145_001721"
     CDS             10378..10593
                     /locus_tag="OO145_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5466865.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            10710..10991
                     /locus_tag="OO145_001722"
     CDS             10710..10991
                     /locus_tag="OO145_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466866.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            11026..11187
                     /locus_tag="OO145_001723"
     CDS             11026..11187
                     /locus_tag="OO145_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466867.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            11174..12004
                     /locus_tag="OO145_001724"
     CDS             11174..12004
                     /locus_tag="OO145_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5466868.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            12001..13377
                     /locus_tag="OO145_001725"
     CDS             12001..13377
                     /locus_tag="OO145_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5466869.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            13450..13656
                     /locus_tag="OO145_001726"
     CDS             13450..13656
                     /locus_tag="OO145_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466870.1"
                     /translation="MAEFMLVACKCVGVGWILLTFFIVLHSYIRLVNDGKDPWYTLFG
                     AAFVWVIIGVMPVVVAKMAWRFVS"
     gene            13674..13949
                     /locus_tag="OO145_001727"
     CDS             13674..13949
                     /locus_tag="OO145_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466871.1"
                     /translation="MDESRKQFLEFAKKQNLSLAYGDCGYVYSSTEMAWRAWQASRAA
                     IEITAPKFIDSREALAKGFTVDYSNGFGDAMDAYEENIRAAGIKVKE"
     gene            14149..14559
                     /locus_tag="OO145_001728"
     CDS             14149..14559
                     /locus_tag="OO145_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5466872.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGKGVRFTDRRYE"
     gene            14556..14738
                     /locus_tag="OO145_001729"
     CDS             14556..14738
                     /locus_tag="OO145_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5466873.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRERARHTR"
     gene            14735..14905
                     /locus_tag="OO145_001730"
     CDS             14735..14905
                     /locus_tag="OO145_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR5466874.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     PNSSMYEEEDDE"
     gene            14898..15167
                     /locus_tag="OO145_001731"
     CDS             14898..15167
                     /locus_tag="OO145_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466875.1"
                     /translation="MSDFSELISFKKDREEMRTESVYYVQHRNKRSVLDQELVITGDL
                     AFRTYKASMEMKDFPKCGSEREAALKLAEWMQRMAAAIENYWSEP"
     gene            15164..15778
                     /locus_tag="OO145_001732"
     CDS             15164..15778
                     /locus_tag="OO145_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCR5466876.1"
                     /translation="MMAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSK
                     EREKAEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPC
                     ISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRI
                     GQEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            15775..15981
                     /locus_tag="OO145_001733"
     CDS             15775..15981
                     /locus_tag="OO145_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HCR5466877.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            15959..16630
                     /locus_tag="OO145_001734"
     CDS             15959..16630
                     /locus_tag="OO145_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR5466878.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTQKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSECGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGAWA"
     gene            16621..17139
                     /locus_tag="OO145_001735"
     CDS             16621..17139
                     /locus_tag="OO145_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5466879.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKLM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKNLDMQLAI"
     gene            17736..18059
                     /locus_tag="OO145_001736"
     CDS             17736..18059
                     /locus_tag="OO145_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5466880.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            18043..18519
                     /locus_tag="OO145_001737"
     CDS             18043..18519
                     /locus_tag="OO145_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5466881.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGMVREIEV"
     gene            18516..18953
                     /locus_tag="OO145_001738"
     CDS             18516..18953
                     /locus_tag="OO145_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5466882.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVSYLQEYIRTQCLK"
     gene            19559..19801
                     /locus_tag="OO145_001739"
     CDS             19559..19801
                     /locus_tag="OO145_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR5466883.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            19881..20369
                     /locus_tag="OO145_001740"
     CDS             19881..20369
                     /locus_tag="OO145_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5466884.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            20347..21843
                     /locus_tag="OO145_001741"
     CDS             20347..21843
                     /locus_tag="OO145_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5466885.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPDDGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVINAMDFGWDHPQAHIQLW
                     WDKDEDVIYLSRVWKAKQKKATEAWSAVKAWSKNTPTAWPHDGHQHEKGGGAQLKEQY
                     ADAGFDMLPDHATWPDGGNAVEPGIAEIRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDILSAVRYGYMMRRFAIQMRDIKDPKEIDYSSYNIPCGVG"
     gene            21843..24044
                     /locus_tag="OO145_001742"
     CDS             21843..24044
                     /locus_tag="OO145_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5466886.1"
                     /translation="MADDRKMADWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGA
                     QWEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEA
                     LAEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISL
                     LPVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLFRDDTGTQ
                     YDWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELI
                     GERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAM
                     DAQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLK
                     NKNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVD
                     SIFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTG
                     EVVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDL
                     VSSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMV
                     AAQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIRE
                     ALKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQKQPQQ"
     gene            24135..25028
                     /locus_tag="OO145_001743"
     CDS             24135..25028
                     /locus_tag="OO145_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5466887.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            25047..26300
                     /locus_tag="OO145_001744"
     CDS             25047..26300
                     /locus_tag="OO145_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5466888.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSSKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGTATSLTLAAEPAHKVEAKDANDMPVDNRQGTITVSASGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYVNVDANAANGAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            26342..26530
                     /locus_tag="OO145_001745"
     CDS             26342..26530
                     /locus_tag="OO145_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466889.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKHKVAKAEEDGENEG"
     gene            26511..26972
                     /locus_tag="OO145_001746"
     CDS             26511..26972
                     /locus_tag="OO145_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HCR5466890.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            26982..28400
                     /locus_tag="OO145_001747"
     CDS             26982..28400
                     /locus_tag="OO145_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5466891.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQSAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEDNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            28400..>29115
                     /locus_tag="OO145_001748"
     CDS             28400..>29115
                     /locus_tag="OO145_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466892.1"
                     /translation="MADSNLNTPVIVQATRLDTSILPRNIFSQSYLLYVIAQGADVGN
                     VANKANEAGQGAYDAQVRNDEQDVILVDHEIRLASAEAKIQDHETRITNAEAAIVGLD
                     SRLTTAENDIDYLTDEVVSIQNTLSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNEDKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTT"
BASE COUNT         7600 a         7018 c         7103 g         7394 t
ORIGIN      
        1 acccatctct tatggagccc ctgctgtttt cttaatatct ggcatgcggt ttcgttaaga
       61 ggaactccga tcgcattgcc agcttttgtt tcatcggggt gcatccatgc cattttctta
      121 tccagatcga cctgtgacca ctcaaggtct gtaacgttgg aacggcgaag gcctgtcgtg
      181 attgcaaaca tgaccacagg gaagaaatga ggagcaattt ctgcaaacag gcgcttcgat
      241 tcctcctctg taagccatct gattcgtcca ttcttaacgc gtggtgttga tattttgggc
      301 gccctgtcaa gccatcccca ttcaacagcc atattgagaa tagcgcgaag tattgccaga
      361 tgccgcgtct tcgttccttt gcttgccagc tttggtttat actccggcac tggcttgcca
      421 agccgcaaac acctgtcccg gctcatttcc cagttcaggc gatggcggcg gttttccatc
      481 ccgtctaccg cctccattat tttttctgtt gttatgtcag agagaatggt ttctctgaag
      541 tgcaacatcc agaacgatat aatgctcttg tcatcatcaa tggacttctt atccgatttc
      601 tcacgcagcc accgtatgca ggcttccttg aatagctttt tcggcgattc cccgagattt
      661 tttactctcc acgcttctgc tttcagacga tcgtgaagtt cttgcgcttg ccttttgtcc
      721 gatgtttcaa gagagcgtct aactcttgat ccatctggcg cgacgaaatc gcagtgccac
      781 gtgccaccgc gtagtttgat tgacatgctt taacctcctg cacatcaacc gcattcaccg
      841 cgctattgtg tctcacagac ttaagcgccg caatgcagtc tgacttgcaa atgcgatatg
      901 gacttttagg tttatctgga tttatctttg cggcctgaag tcgtccactt cgtatccact
      961 gtgtgatagt gcctttgtct accttcagat acgacgcagc ttcttcacga gtgaagattt
     1021 cttcttccac ctggaatctc catttgttgg attgacatga ttgcggtagg tctggatatc
     1081 ttgagaaatg aactggcctc atcgagtgtg aggctgtgtg attccatagt tagtccttgc
     1141 gtagctcgct gattcttctg taagtctctg gtgctttgtt tccgtgtatc ttcatttcag
     1201 acttcaacag agcaacgagg gaatcccatt cgttgaggat gcctttgaat gccggaacgc
     1261 gctttgcaac cttgccgaat gaatctctga tttctggaat ctgctcaaca agtgcaacgc
     1321 atcgtctgaa atcggctgcg tcatggggag cgccgaagtg atgaccatag atattctttt
     1381 tcagcccaca tgcgattgag gcaagagttg cgctactgat gccaacatcg ccagtcgatt
     1441 gccatttcaa aaccttcata gccaaatctg acatttcttg tctccaataa aaaaaccgcc
     1501 atcaggcggc ttggtgttct ttcagttctt caattcgaat attggttaca ttgttttcat
     1561 atatgaataa ataaattagc ttttttcgtt gccttcgctt tctttattaa ttttaacaaa
     1621 ctcgttttta ccacgctctc caaatgcgtc tttagagtcg ttgtatccgc aatcgcagca
     1681 cacataatct ccagaccatc cacgcattgt tttttctttt gcaatatttc cagaaccgca
     1741 ttttggacaa gacatgtcac tacctccaaa gcatgagtga gatgacaacg taacattgat
     1801 tggagattaa caatagattg ctgatgtaaa agatatgtat aagcttcgct ttcaaagtgg
     1861 aggctctggt agcggcatcc agtgtgacgg ctcacatacc ccctcaacac cattcatgta
     1921 aaagaattga aataaccctt tacctttgtg aaaccctacc atctgctctt ttgtgtctga
     1981 gcaataaacc aaaacatctt cttcgtttgg cattcgatca ctacagctta tccaaccatc
     2041 aggagttacc ggagagttgc caccgggaat attttccgga atattttgtt gtgcgttttg
     2101 tggttgttcg gatttaccct gaagcatggc ggcgcggcag gcgttccagc cagctgttcg
     2161 cccaagcgcg taaacttcag atggctcaag ataatcaatg tcatgcccat cctcatcgtc
     2221 gttctcaggt aatgcagcag gtactaccgg tactggcgga gcggcgtaga cttcaataat
     2281 cccattatca ataggccatt ccccatcctt gatgtagtca cttgtgccgt caacttgctg
     2341 ttctgcaatg tggaatgcgc caataggctc tgcttccagc gatgccagtg cgatacgcag
     2401 ggcagccagg atattcccct ggtaatcatc aagcccgaaa ggaagctcat cacgaacgct
     2461 ttcataatcg ttgatagcct gctgcagcca ttctcttgtg atagtgctca tgatgcctct
     2521 cctttacatg cttcggtgac gtttattcca gcgttgtgca gtgcctcaag aacctgatgc
     2581 tgcctgtaaa ccatttcagt gtggtaaggc tcgtcaaaat cgacgcgatg caacatgcta
     2641 tagcattgcg gaagcacaac ctcccgcaac tccagctcag caatgcgctt ctctgcggct
     2701 tccagcttct cgcgcatatc gtcaacgtac tcgaccagag atccgccagc aggaatttcg
     2761 cactcctcga ccagttggaa gtagatatca gctgcggccc gtgtgttgct atgcctagcg
     2821 tcgcccatct caccttcacg aagagcatcg cgttcggcgg taagattggc tattttgctg
     2881 tctttgcctc ccagctcaac tctcagcttc cctaccgtta gcgcaatatc ctcgttctcc
     2941 tggtcgcggc gtttgatgta ttgctggttt ctttcccgtt catccagcag cgccagcacg
     3001 gtagctggat tggctgcggc gatgaattca gcattggcct gctgttctat ttggaaatct
     3061 tcatcgaaac cgctttcagg atgcgctcct tcaattctgc aaatgggaat atatccagca
     3121 gcctcgcgat gaattagcgc atcatcacca tcaaatcggc cctctccata ttcgagcgac
     3181 cactcgccac acgttgcttt ctctgccttg gcacgcagtg cctgatagtc aatcttgctc
     3241 actgtttgcc tcctttgctc gctgattcca ctctgctcta acctctgaat aaaaaatcgc
     3301 gcagtcattt ccaggcgctg catatttgct accagattga gcgcgacacg taccgcatcg
     3361 aacgaaatag aatcgaccgc cagagccata ttcagggtga tctgcttcgc tggcaacgtg
     3421 cgctgcaccg ccacagaatg gacatggtag taggttgctc atgaatgcac tcccttgtga
     3481 agttgttccg cacaatgcag cagtgcgtca gtcgcctctt tcaccgtaac gcagtcgcca
     3541 tcgtccagcc cggacaccga cgcgtgctta acgaacgccg cgcaaaggtc atcaaacgcc
     3601 ttagcccgga cttcagccag gaaagcatcg gtggctgggg tttcgatatt aggcagaagc
     3661 gcataatcgc aaatcgtatc gatcgcagga tcgcaacagt catcctcgtt gcgtggtcgt
     3721 tcgccaacct tcgttgacga ttgcatgatg attccccagc aaatactatc gacctcttcg
     3781 ctccatccat cgcaagcatc accgcgatag tcgtcaattg cagcctcagc cgattcgata
     3841 gcttcctcag cagttttgtg ccattcgaaa ttatgctcag agccatatga gaaatatgaa
     3901 tgtcccgcct tcatcaccgc actctccgct gccagcgccg cgcacttggc atcaaggtta
     3961 tcaatcgtga ttccagcaga acgacactcc cgcaacgccg tttctagttt tgattcaagt
     4021 tcaccgaact tacgcaccag atattcagcg tttgtttcgt taacatttaa atctcggggg
     4081 atgcatttac ctttcagaaa tccatccatc tcaattagtg tcatttgttt cattttttcc
     4141 cactccgcca catcgcattc agatatttgt tttgatttac tgacggaaaa gaatttctct
     4201 taagcaattc ctctctcgat ggcattggct ttaggcgttg gcgaataatc atttctgccg
     4261 gaagaatgcc gggattgtat gcaagtcctc tcatggtaaa ttcctcagtc attactgata
     4321 gcgccatagc gtgagcggta attacgcagg cgcgggtcga tatattcagg gaagtgggta
     4381 tatgttgctt tgcggaacgg tcggattgat gtctggtaaa ttcgctcgcg ttcttctttc
     4441 tctgcaagcc atatacaatg gcgaatttcc tttttctctt tcgtttcctg cggtagcgac
     4501 attatccggt cgtagttttt tctgaattta tccagcacct ccaatacgga attgccggaa
     4561 cagcggcggg gggcatccgc accatacaga ggcgctggca tgtttttctc ctgttgatta
     4621 tttagctaac tttttccaga tcgctgaaac gtatttggct tggtgaatgg catcatcaag
     4681 cgcgttgtgg cgagttcctt tgaatggcat atctcgctta gggtcgaatc ctattgcctt
     4741 cccaagttcg acgattgttc ttacgtcgcg gtcattccac cactgccacg gaactggctg
     4801 ccctgtcagc gaataactgt tgcggagaat aacgcagtca aatgatgctc cattccccca
     4861 aacctgaacg aatttgtggt tagcgttctt tatgatgaat tcagataacc atgaaagagc
     4921 cgttgaaagc tcttgagtgt tgctggttag cgattttctg gcttcttcac tctgttccat
     4981 ccaccataaa atcgttgaag cgtcaggacg cgcccgatat cgcattgatg actcaagcga
     5041 gatatttacc gagaactctt ctcctgtttc tccggtattc gggtcaaaga ataccgcccc
     5101 aatagaaata actggcgcgt atggcccgtt gcccattgtt tcaaggtcaa ccattaagtg
     5161 attcatgtaa gtccttaaat tgcgtgaata gcgtgacgag ggaaggggag agttactggt
     5221 gcaaagggta tatcgtcgtc aaaatccatc ggaggttcgt tgtgttgtgc tggtgatgat
     5281 tgctgctgtg gcttctgtga ttgcctgctg gctgcttgtt gtttgctgtc gccaatgccg
     5341 ccaagcattt gcatcacgcc attaattccg acatgaacct cggttgtgta acggtcttgc
     5401 cctgactggt ctttccactt tctggttctc agcattccct cgaaataaat ctgatcacct
     5461 tttttcacat actgccccac gacctcagcc agtttcccgg atacagcaac acgatgccat
     5521 tcagtcaatt ccttttgctc gccagtattt ttatctcgcc attgttctga cgtggctatt
     5581 gtcaggttag cgaacgctgt tcctgatggt gagtatcgaa cttccgggtc ttgtcctacc
     5641 cgaccaagga taatcacctt atttacgcct ctgcttgcca tttatgccgc ctgttttagt
     5701 tcgttaactc tgatgttcat tacctgaacg catttagcct gcgcatcctc attgccagcc
     5761 attaattgcc agtcatgctg ataacgttct atgagttttt tcttgtcagt ttctgttgat
     5821 gcataatcgc tgaagtcttt caggatttgt tcgcagtcaa ccgatggaga tttctggttg
     5881 gtattttctg gtgatggttt gttatctgat gctgggattg cccatcccgg cagcgatgga
     5941 gggagccagt aaaatcctgt tccatccttg agttttgccc tgtgccatcc ctgcttttta
     6001 tcgagagatg tttgtgcgaa accttcctca aggttataca gataccgacc gattccccac
     6061 tgtacggcag cgcgcttcat tgcaccggaa cgaccacctt tgacggcttc tacctgcgtg
     6121 ttttcagcag catcccattt ggttacccat tcggaatcaa tctttattga tatgccgcat
     6181 tcaacgccgc cgttgttggg aatatcgcgg tattcattgc gccatcctgc tttgccgcaa
     6241 acatcgtcca ggcgtttcat gattgcccgg ttcgtgacat aagccagcac catagcccac
     6301 actttgccat cgcgtgtttt accgctttgc tgtattcgcc attcgatatc ttcagggctg
     6361 aatggctcat cgaatttatt caaattcata attcacctca gaatggacac ggcccaagga
     6421 aataacgctg atttaatact tcgactcggg acaaattaag gcatacccgc attccttcgc
     6481 ggtcgccatt atggcgatac cagagagctt tctgcgtgta catgcgtctc tgtaacttgc
     6541 tctccttcac tgtggttgca agtgacatga atatctcctt cgttaccgat taattctttc
     6601 atctgacgaa tgaattcttc gtctgaccag ttatctgtaa aactcatttc ctgcgatacc
     6661 acggaaggtt gatagctgat ttcatcgctt tatttgcttc aagccacatt ttagaatcac
     6721 caataaatct ggctattact gctttgtttt gtgctgcacg aagcatctgg tgattgatgg
     6781 ctatttcatt gcgcataata agacctcaac tcttttccat ccgtcacgta atttacgggt
     6841 gattcgttca agtaaagatt catttagttg gaaggcaccc atgcgagcgc ctcccgcgat
     6901 tgcgtaaatc atgggtggtt ccttatgttg gttttattag taggttattt ttgttgcgaa
     6961 tacttcgcct tttacgatgg ctgttatgat atttttagca acattttctg atgcgccaac
     7021 cttgataagg tcagcaagta ttttgttatt tacttctttc cggtgagctt tatcctttgc
     7081 tctgcgctct tcttcgtcat tgattctttt ttcttctgct attctggctt gctcttttgc
     7141 ttcagcctcg cgacggattc gttcagcctc ctcctgtgct tttctgcgtt ctgcttcaat
     7201 tgctgcctgc ttttctcttt cagctcgttc tgcttcctct tttgcttcgc gctgtgctcg
     7261 ttgctcggct tcaatgcgct cacgctctgc acgctccgct gcggccttag cttctgcttc
     7321 tcgccttgct gctgcttcaa tttcggcttt tgcctttgct tcggcttctg ctctggcttt
     7381 ctcttcagct tctcttttta agcgttcttc atgctctcgc ttttcctgct ccgctttgag
     7441 tcttgcctct tctctttggc ggtcaaattc gcgatccatc aaaatcgcta tttcatggtc
     7501 agactcaatt tgctttgcga gagcttcagc tgctgcctta gcttcttctt cggctttaat
     7561 ccgtgcctgt tcctcctcat aatcagtaag aggctggcga gccttggatt tcagttcatc
     7621 aaggcgatcg cgcactgtct tgcggttggc atcaattagc tttggaattt ccttcagttc
     7681 agcaacaagg tctttgccaa gaccatcgag atatgttttc gtctgcgcaa ctttatacgc
     7741 cagagaagcg atctcctttc tgccctttgc cgttgtgata tcaggcacaa aggacataac
     7801 ttcacgttca accttttgaa gaatttcttc aatctggtcg gcagactgaa atacagtcat
     7861 tgcatttgct ttttcaataa caactaaatc tgttacttca ctcatatatc ctccgtcaaa
     7921 aaattgccct cacactggag ggcaaagaag attttcaata atcagaacaa gtcggctcct
     7981 gtttagttac gagcgacatt gctccgtgta ttcactcgtt ggaatgaata cacagtgcag
     8041 tgtttattct gttgtttatg ccaaaataaa ggccgactat gcggcctgaa attacttaac
     8101 caatgatgct gcatattcga taaggtaaag ctttggagcc agccaaattt ttaaccaagt
     8161 catattggtt actgcaccaa taataaaaat cccccacaga gccaaaactc caaccaatgg
     8221 catgataaga agattaatat cacctttgct atcccaaacc attgtcggcc tgtatttggg
     8281 atttcccctc tcccatgagt atccttcatc accgatttta cctgtctcaa ctctttggca
     8341 ctgcttcttc ataaaccaga aaaccagtgg gattgttaga atggctatta atgttttaat
     8401 cagactgtca accatattcc atagcagcaa ctgatgaaca acatcaggaa tctgtgcctg
     8461 gctaaatgaa acagccgcat ctattccatt gctggctttt tgcagtagtt ctacgagaat
     8521 cttgtttgct tgttcttcca tatctcacct caaataagtg gtttgctgcc taatttcatt
     8581 ttctggcgac caacacaagt cacacccatt tcactgcgtg gcttgctgta ccatgtgcgc
     8641 tgattcttgc gctcaatacg ttgcaggttg ctttcaatct gttcgtggta ttcagccagc
     8701 accgtaaggt ctatcggatt cagtgcgctt tctactcgtg atttcggttt gcgagtcagc
     8761 gagagaatag ggcggttaac tggttttgcg cttaccccaa ccaacagggg atttgctgct
     8821 ttccattgag cctgtttctc tgcgcgacgt tcgcggcggc gtgtttgtgc atccatcttg
     8881 attctcctgt cagttagctt tggtggtgtg gtaggtggga gacccatttc gacctgcttc
     8941 ggccgacttc aattcggcaa tagttccgca ggcctcgccg ctttacgtgc gacatattcc
     9001 catccatgaa cccttcacca caccccaaag ccttctgctt tgaatgctgc ccttcttcag
     9061 ggcttaattt ttaagagcct caccttcatg gtggtcagtg cgtcctgctg atggcttaaa
     9121 actacaagaa agattgtatg ctgtaaacaa gaaatattgt aaaaaggggc gtaaaaaaca
     9181 aactccattg tttttaaatg aaaaatagtt tgtttttggt tatcgagatt gaggtgggga
     9241 ttactggttg caggttccga ctacatcacc aacaaatgat ttggttgatg taagttgttg
     9301 catacctggg atattcatta ctttggagta aagagctttt ttgtctgtag tgattgatca
     9361 agtttcaacg gttattcctc ctccagactg gtattctcct accatagtgt tcgatgacaa
     9421 agcagtgtat ttcatctctg gatagacgcc agaaactgat tcataaactg atgatttatc
     9481 gccatttatt gttacgtgga aaacggaatc ttccgtgctg tcttttgtaa actcgtaacg
     9541 atcgccattc attgacccgt acccgtgcag gtttgtgaca atccagcatt cagaattggc
     9601 gctggtagtt aagagtattg agagtagcgc cgcaatcctg atcatacgaa ttttaccctc
     9661 gcttccacga caacaccgat aatcttgcag ttcccgttga taggagtcag aggccatgaa
     9721 gggttcaggc ctttcaggta cttctgcccg ccatctataa ccagtttctt gaatgttgct
     9781 tcgttcgcgt cagtcagttt ggctacaaca aggcttccat tcactggctc gcgtccagta
     9841 tctactaaca ccatatgacc ttcagggatg ctttgaccta caggtgaggt catggaatca
     9901 ccttcaacct tcagccagaa tccatcgcct aataagttaa cgtcactgtc ataccattca
     9961 tcaatgtcct tgatatcgta gggttcacaa gcttcacacc acgaaccagc tctaaccatg
    10021 ctaatcaatg gatatttccc tttgggctca acatgcccaa caaatctaac attcgaatca
    10081 gaggtgccat tgagcagcca gtcaacactt acgccaagag ctgacgcaag ttctggtaaa
    10141 aagcgtggtc gcttagtttt accgttttcg agctgctcta tagactgctg ggtagtcccc
    10201 accttttgag caagttcagc ctggttaagt ccaagctgaa ttcttttgct ttttaccctg
    10261 gaagaaatac tcataagcca cctctgttat ttacctccaa tcttcacaag aaaaactgta
    10321 tttgacaaac aagatacatt gtatgaaaat acaagaaagt ttgttgatgg aggcgatatg
    10381 caaactcttt ctgaacgcct caagaagagg cgaattgcgt taaaaatgac gcaaaccgaa
    10441 ctggcaacca aagccggtgt taaacagcaa tcaattcaac tgattgaagc tggagtaacc
    10501 aagcgaccgc gcttcttgtt tgagattgct atggcgctta actgtgatcc ggtttggtta
    10561 cagtacggaa ctaaacgcgg taaagccgct taagacattc ccgctcttac acatcccagc
    10621 cctgaaaaag ggcattacca gaaacaaatc tctatggttt tgcgtttctt tgcgaagcca
    10681 actctatcta atcattaagg aaattatcta tgggtactat tgcaactaaa agcaagaaag
    10741 cggctcgcat cgagtcagcc ttgctgaaca aactggcact gatggggcag aagacattcg
    10801 ctcgagcaat gggggttcct gaatatcagg taagccgatg gaagaatggt ttcttctcgc
    10861 aggtaagcat gatgcttgct gttctggaat acggaatcga agacgatgaa atggctgaat
    10921 tgactaagcg gcttgccgat tacctgacaa aagaaaaagc cccgaagaac ggcgaattct
    10981 tcgaggccta atgtagaaag actggatcaa tccacgggag taattatgac aaaacgtcgt
    11041 aagaaatacc aggaaaaaga agagattcga caccctgatt cacctgaggg attagtggta
    11101 gccgcagcaa ataacagggc gttcgcagag cgccttgttg gtgtttacag actagccaaa
    11161 gcaggagtga aacatgggcg tcgttaagtt agctgattac aggcctcaac tggaggtcgt
    11221 ggagcatcgc gtggcagaac tcgaagatgg ctacactcgg actgcaaaca cactgttaga
    11281 agccgccatg ctttctggac ttactctaca tcagttactg attgttatgg ctgtgtggcg
    11341 caagacatac ggttacaaca aaaaaataga ttggatcgga aacgaacagt tcgctgaact
    11401 cactggcatg gcaccaacca aatgctctac cgccaaaaac gagcttatca gaatgggagt
    11461 tctcattcag gtggggcgtc aagttggtat gaatacaaac atttccgagt ggaaaacgaa
    11521 agttaacgga ttcggtaaaa catttaccaa ttcggtaaaa caaaccttca ccaaatcggt
    11581 aaaaagcaat ttaccgaatc agtcaaacac aaaagacaat atacaaaaga caataaatac
    11641 aaataccccc ttacccccta acgggggcgg cgatgggcag gttaaacctg aacgtcgcaa
    11701 ggcagaacga atcgactacg aatccttcct gaacgcctac aacaccgaag tcggtgacag
    11761 actgccacac gctgttgcgg tcaacgagaa acgcaaacgc cgcctgaaga aaatcatccc
    11821 gcaactgaaa acgccaaacg tggacggttt cagagcgtat gtcagggcgt ttgtgcatca
    11881 ggccaagccg ttttacttcg gagacaacga cacgggctgg acggccgatt ttgattacct
    11941 gctgagggaa gactcgttaa cgggggtacg ggaagggaag tttgcagaca gggggattgc
    12001 atgaaacagg atatcgaagc gagcgttatc ggtggcctgc tgattggtgg attaacacca
    12061 accgccagcg acgttctggc aacgctggag ccggaagcgt tttcaattcc gctctaccgg
    12121 aaagccttcg aggttatccg caagcaggcg cgaaacagaa acctaatcga cgcgctgatg
    12181 gttgccgagg cgtgcggaga ggagcatttc acgtcaatcc tgatgaccag caagaactgc
    12241 ccgagtgccg caaacctgaa gggatatgct ggaatggtcg cggataacta tcaccgccgt
    12301 ctggtgctgg aaatcatgga tgaaatgcgt gaaccaattc agagcggaac catcgacgca
    12361 tcgagtcagg cgatggatga gcttgtaaag cgtctttcag ccatcagaaa gccccgtgac
    12421 gaagttaaac ctgtacggtt aggggaaatc attactgact acactgacac gcttgacagg
    12481 cgtctgagga acggagaaga gtccgatacc ctgaagaccg gaatcgaaga acttgatgcc
    12541 atcaccggag ggatgaacgc ggaagacctg gtgataatcg ctgctcgtcc tggtatgggg
    12601 aaaaccgaac tggcgctgaa gattgccgaa ggcgttgcaa gccgcgttat tcctggttct
    12661 gacgtccggc gcggagtgtt gattttctca atggaaatga gcgcattgca gattgcagag
    12721 cgaagcattg ccaacgccgg gaggatgtcg gttagcgtgc tacgaaatcc tgcatcgatg
    12781 gatgacgaag gctgggcgcg tgttgctaac ggcatgagtc agcttgcaga tttggatgta
    12841 tgggtagtcg atgcctcgcg gttatcggtc gaagaaatac gctcaatcgc agaacggcac
    12901 aaacaggaaa atccaaacct gtcactcatt atggcggatt atcttggcct gattgagaag
    12961 ccgaaagcag accgcaacga cctcgcaatt gctcacatct ccggaagcct gaaggcgatg
    13021 gcgaaagacc tgaaaacgcc tgttatctcc ctgagtcagc tttcgcgcga tgttgagaag
    13081 cgaccaaaca aacgcccgac aaacgcagat ttgcgtgatt caggaagcat tgaacaggac
    13141 gcagactcaa tcatcatgct ctatcgggaa gcggtatatg acgagaacag tagcgccgca
    13201 ccatttgctg aaatcattgt gacaaaaaac cgttttggct cgcttggtac ggtttaccag
    13261 cggttctgca acggacactt tgttgcatgt gaccaggatg aagccagaca gatttgcaca
    13321 gcatcaaatg cgcccgctgc gcgtggcaga cgatatgcac aaggggctga cgtatgacca
    13381 tctacgtcac tgagctaata acaggcctgc tggtaatcgc aggccttttt atttggggga
    13441 gagtaaatcg tggctgagtt tatgctcgtc gcatgcaaat gcgttggcgt tggatggatt
    13501 cttctgacgt tttttattgt tctgcatagc tacattcgtc ttgtgaatga cggtaaagac
    13561 ccatggtata cgttgtttgg cgctgcattt gtctgggtga ttatcggtgt tatgcctgtt
    13621 gtcgtagcaa aaatggcgtg gcgttttgtt agttgaactg agggtaagta tcgatggacg
    13681 aatcaagaaa gcagtttttg gagttcgcga aaaagcaaaa tttaagcctt gcatatggtg
    13741 attgcgggta tgtttattca tccacggaaa tggcatggag ggcatggcag gcatctcgcg
    13801 cagctattga aataaccgcg ccaaagttta tcgacagcag agaagcatta gccaaagggt
    13861 ttactgttga ttattccaat ggcttcggtg atgcaatgga tgcttatgag gaaaacatcc
    13921 gcgctgctgg aatcaaagtg aaggagtgag catgagtcga cgaagtagct ttttggggtt
    13981 tgtaatattc ctgtcctgca ctggttacat cgtaatctgg tcaatttcga acattgaccg
    14041 tggcggggaa tatctcattg taatgttctt tcctttgttt cttgggtggt acgccgcaag
    14101 gttgctggaa gaatggggtt acaggcataa aaaataaagg agtattcagt gaagcaaaca
    14161 atcttcctcc gaactaagca acaacagcaa gctgcaataa atgccatcct cgcaacacca
    14221 ctcgataaag acaagccagt caccatccgc attactgact acaagcgcaa ccttgaccag
    14281 aacgcaaaat ttcacgcgat gctggcggat atcgcacgtc aggttcaatg gtgcgataaa
    14341 tggttaaaac cagaacaatg gaaggttttg ttgataagcg gtcatgcagt ggcgacaaag
    14401 caggaagctg atgttttgcc cgggcttgaa ggcgaatacg tcaacattcg tgagagcagc
    14461 gcacagatga gcgtgaagcg tatggcaagt ctgattgagt acacgacagc atgggctatt
    14521 ggtaagggtg tcagatttac cgacaggagg tacgaatgag acgacagcga cgaagtatca
    14581 ccgacataat ctgcgaaaac tgcaaatacc ttccaacgaa acgctccaga aataaacgca
    14641 agccaatccc aaaagaatct gacgtaaaaa ccttcaacta cacggctcac ctgtgggata
    14701 tccggtggct tagagaacgt gcgaggcata caaggtgatt gacccaaatc gaagttacga
    14761 acaagaaagc gtcgagcgag ctttaacgtg cgctaactgc ggtcagaagc tgcatgtgct
    14821 ggaagttcac gtatgtgagc actgctgcgc agaactgatg agcgatccga atagctcaat
    14881 gtacgaggaa gaagacgatg agtgatttct ctgagcttat ttccttcaaa aaagacagag
    14941 aagaaatgcg gactgaatct gtctattacg ttcaacaccg gaataaacgc tctgtgcttg
    15001 atcaggagct ggttattacc ggagacctgg cattcagaac atataaggcc agcatggaaa
    15061 tgaaggattt ccctaaatgt ggttctgaaa gagaagccgc gttaaagctg gcagagtgga
    15121 tgcagagaat ggctgctgca attgagaatt actggagcga accataatgg ctaaaccagc
    15181 gcgaagacga tgtaaaaacg atgaatgtcg ggaatggttt caccctgcat tcgctaatca
    15241 gtggtggtgc tctccagagt gtggaacaaa gatagcactc gaacgacgaa gcaaagaacg
    15301 cgaaaaagcg gaaaaagcag cagagaagaa actacgacga gaggagcaga aacagaaaga
    15361 taaactgaag attcgaaaac tcgccttaaa gccccgcagt tactggatta aacaagccca
    15421 acaagccgta aacgccttca tcagagaaag agaccgcgac ttaccatgta tctcgtgcgg
    15481 aacgctcacg tctgctcagt gggatgccgg acattaccgg acaactgctg cggcacctca
    15541 actccgattt gatgaacgca atattcacaa gcaatgcgtg gtgtgcaacc agcataaaag
    15601 cggaaatctc gttccgtatc gcgtcgaact gattaaccgg atcgggcagg aagcagtaga
    15661 cgaaatcgaa tcaaaccata accgccatcg ctggactgtc gaagagtgca aggcgatcaa
    15721 ggcagagtac caacagaaac tcaaagacct gcgaaatagc agaagtgagg ccgcatgacg
    15781 ttctcagtaa aaaccattcc agacatgctc gttgaagcat acggaaacca gacagaagta
    15841 gcacgcagac tgaaatgtag tcgcggtacg gtcagaaaat acgttgatga taaagacggg
    15901 aaaatgcacg ccatcgtcaa cgacgttctc atggttcatc gcggatggag tgaaagagat
    15961 gcgctattac gaaaaaattg atggcagcaa ataccgaaat atttgggtag ttggcgatct
    16021 gcacggatgc tacacgaacc tgatgaaaaa actggagacg attggattcg acacccaaaa
    16081 agacctgctt atctcggttg gcgatttggt cgatcgtggt acagagaacg tcgaatgcct
    16141 ggaattaatc acattcccct ggttcagagc tgtacgtgga aaccatgagc aaatgatgat
    16201 tgatggctta tcagagtgtg gaaacgttaa tcactggctg cttaatggcg gtggctggtt
    16261 cttcaatctc gattacgaca aagagattct ggctaaagct cttgcccata aagcagatga
    16321 acttccgtta atcattgaac tggtgagcaa aggtaaaaaa tatgttatct gccacgccga
    16381 ttatccctgt gacgaatacg agtttggaaa gccagttgat catcagcagg taatctggaa
    16441 ccgcgaacga atcagcaact cacaagacgg gatcgtgaaa gaaatcaaag gcgcggacac
    16501 gttcatcttt ggtcatacgc cagcagtgaa accactcaag tttgccaacc agatgtatat
    16561 cgataccggc gcagtgttct gcggaaacct cacattgatt caggtacagg gagaaggcgc
    16621 atgggcataa gagaactaaa ccttaccaaa gagcagcacg agtggctgaa tggctggctt
    16681 gaactgtggg gcgcatgggt ttattcaggt cgtctggaaa agcgcatgag cagcgtaata
    16741 gcgaagctca tggagagcgt agagccggga agagttatga caaggccaat gtgcaatgat
    16801 gatgatggaa tgttgatttc tcaggtcgtc gattccgtca tgtacattga caaaaaagcc
    16861 tttggcatcc tcctcagcta ctacgctcat ggttcatcta agcgagcaat tgcatcctac
    16921 tatcacgcga ctgcaaagcc acgcaagatg tgtggacgtg gtggcgaggg atggagaaaa
    16981 ccttcactgg caacctgtag aaacgaaatt gacgacatcc tgaaagcgtc attatttgtt
    17041 ttgtaccaac caatgcaaaa tgctttcaaa atgcgtaaac gtgttgagaa agttaagcat
    17101 gttgctgtta aaaaccttga catgcaatta gccatttagc cataataaga gggtaagctg
    17161 ccgttagtga ctcttaagtt gcaacggtgg cttttttgtt tgcacaacag gtaagagcat
    17221 tgaacccgca gacctcgcgg aattggtgaa aggtgccgcg cagtgctctt atcgttgtgg
    17281 tgaatacgca ggctgatgcg ttaatcaggt gaacgagaca cccgccggtc cgtgatatgg
    17341 cacaccgtgc cggtcatatc tgccgcggtt aggtttacga ggatttcgta aagctggtct
    17401 agggtgaagc cgtgaaagcg gaggaagtaa aacgaggcgt cggtacacgc ctatcgtcat
    17461 taagtcggag ttcagcaccg accgccacaa cccaaactga gccgtagcca ctggctgtcc
    17521 tgaattcatc agtgatagtt atgctgcggc cttctacaca tgaccttcgt gaaagcgggt
    17581 ggaaggaggt tgcgctaaca acctcctgcc gttttgcccg tgcatatcgg tcacgaacaa
    17641 atctgattac taaacacagt agcctggatt tgttctatca gtaatcgacc ttattcctaa
    17701 ttaaatagag caaatcccct tattgggggt aagacatgaa gatgccagaa aaacatgacc
    17761 tgttagccgc cattctcgcg gcaaaggaac aaggcatcgg ggcaatcctt gcgtttgcaa
    17821 tggcgtacct tcgcggcaga tataatggcg gtgcgtttac aaaaacagta atcgacgcaa
    17881 cgatgtgcgc cattatcgcc tggttcattc gtgaccttct cgacttcgcc ggactaagta
    17941 gcaatctcgc ttatataacg agcgtgttta tcggctacat cggtactgac tcgattggtt
    18001 cgcttatcaa acgcttcgct gctaaaaaag ccggagtaga agatggtgga aatcaataat
    18061 caacgtaagg cgttcctcga tatgctggcg tggtcagaag gaactgataa cgggcgtcag
    18121 aaaaccagaa atcatggtta tgacgtcatt gtaggcggag agctattcac tgattactcc
    18181 gatcaccctc gcaaacttgt cacgctaaac cccaaactca aatcaacagc ggccggacgt
    18241 taccagcttc tttcccgttg gtgggatgcc tatcgtaagc agcttggcct gaaagacttc
    18301 tctccgaaaa gccaggacgc tgttgcactg cagcagatta aggagcgtgg cgctttaccg
    18361 atgattgatc gcggtgatat ccgtcaggca atcgaccgtt gcagtaatat ctgggcttca
    18421 ctgccgggcg ctggttatgg tcagttcgag cataaggctg acagcctgat tgcaaaattc
    18481 aaagaagcag gcggaatggt cagagagatt gaggtatgag cagagtcacc gcgattatct
    18541 ccgctctggt tatctgcatc atcgtctgcc tgtcgtgggc ggtcaatcat taccgtgata
    18601 acgccatcgc ctataaagag cagcgcgata aggccacatc cacaatcgct gacatgcaga
    18661 agcgtcaacg tgatgtagca gaacttgacg ccagatacac aaaggagctt gctgatgcta
    18721 acgcgactat cgaaagtctc cgtgctgatg tttctgctgg tcgtaagcgc ctgcaagtcg
    18781 ccgccacctg tgcaaagtca acgaccggag ccagcggcat gggcgatgga gaaagcccaa
    18841 gacttacagc agatgctgaa ctcaattatt accgtctccg aagtggaatc gacaggataa
    18901 ccgcgcaggt tagctacctg caggaataca tcaggacgca atgcctgaaa tgatcgggcg
    18961 atgaaaacca aaaaaacagg agcaatacat gactaagctt tatcaccgca tctcaacttt
    19021 tctctctggg tgctgggcgt ttatcacgtc tatttcgttc gccatcttta gtttcggtag
    19081 cacagcatgc tcgcttagtc ggggtatgtg gcgtgctatt tcagcactag cgccgaaatt
    19141 tttacctgaa aaggctgttt ggcgaattgt agagcgaatg tgtagtgaga gcgttcgcga
    19201 gaagattaac gtatttggac gtcatcctcg aaatacaggc gcattgtgca gttcgttact
    19261 gtagtcatta caaagcccat caatgggtgg gcttgataat gaaaccggaa tttattctgg
    19321 gcaaccagtt acggcagtac cgcgaaacaa cccaagccag taagtgggga aataacactg
    19381 gcagccactg aaagatgaac ctccagcctt atggcaaaaa agattctttg tggtggcgga
    19441 ctgatggaaa gacatcggtt attgcagaga ccattcaatg agtggtctcg acaatggctt
    19501 ataccctaca cgggataact taactgatat cccttttaac ggataaacgg agccaacaat
    19561 ggcagagatt attcccatga ctgaagaaca gaaattccag ttagagattt acaaactggt
    19621 catgaaccag aacgcagctg cagaggaagc atttcagttc attggtactg acgagctgaa
    19681 gcttgagcta ttcaaaattc acttccagtc aggcggcgct aattcagata tcacgacccg
    19741 cactatcgaa gcggtgcgta aatcgaagga agcgttagac ctgttcacca ccggagcata
    19801 aacatggcaa ctcaaggttt cgacaaccca tccaaattcc gcgatgaatg ggataagcaa
    19861 gcagaaggga aataatcaat atggcagcac caaagggcaa ccgattttgg gaggcccgca
    19921 gtagtcatgg gcgaaatcct aaattcgaat cgcctgaggc gctgtgggct gcttgttgtg
    19981 aatacttcga gtgggctgat gataacccgc tatgggaggg taaggtattt tcatatcagg
    20041 gagaaataat taaggctaat gtccctaaga tgcgagccat gactatttca ggattgtgca
    20101 ccttccttga tatcaccagg caaacatggg gaaccttccg gtcaatggaa ggtttttctg
    20161 acgtcacatc acgagcggaa gacatcatct acgaccagaa attctctggc gctgccgctg
    20221 accttctcaa cgctaacatc atcgcccgtg atttgggcct caaagagcag tcgcaagttg
    20281 aagacgtgac acctgataag ggagatcgcg ataagcgacg ctctcgtatc aaggagctat
    20341 tcaaccgtgg aactggacgc gattcttgat aacctgagcg acgaagagca aatcgaattg
    20401 ctcgagctac tcgaagaaga agagaactac cggaacacac acctgctata tgaatttacg
    20461 ccatacagca aacagcgtga gttcatcgac gccgggcatg actatccaga gcgatgtttt
    20521 atggctggta accagcttgg taagtcattt actggtgctg ctgaagtcgc gtttcacctt
    20581 acagggcgtt atccgggaac aaaaggctat ccggatgatg gtaaatatgg cggggagtgg
    20641 aaaggtaagc gtttctatga gcctgttgtc ttctggattg gcggcgagac aaacgagacg
    20701 gtaaccaaaa cgactcaacg catcctgtgc ggtcgtatcg aagagaatga cgagccaggc
    20761 tacggttcca taccgaaaga agacatcatt agctggaaga agtctccttt ctttccgaat
    20821 cttgttgatc accttctggt taagcatcac acggctgatg gcgttgaaga tggcatttca
    20881 atctgctact tcaaaccata ctcgcaaggc cgtgctcgct ggcagggtga cacaatccac
    20941 ggcgtgtggt ttgacgaaga gccaccatac agcatttatg gcgaaggtct tacccgtacc
    21001 aacaaatacg gtcaattctc aattctgacg tttaccccgc tgatggggat gtctgacgtt
    21061 gttaccaagt tcctgaagaa tcccagcaag tcgcagaaag tggtcaacat gaccatctac
    21121 gacgctgagc actacaccga cgagcagaaa gagcaaatca tcgcatccta tcccgagcat
    21181 gagagagagg cgcgtgctcg cggtattcct acgatgggta gtggtcgaat cttccagata
    21241 ccggaagaga ctattaagtg tcagccgttc gagtgtcctg atcacttcta cgtcatcaat
    21301 gcaatggact tcggatggga tcacccacag gcacacatcc agctttggtg ggataaagac
    21361 gaggacgtga tttatctttc tcgcgtctgg aaggccaaac agaagaaggc gacagaggca
    21421 tggagtgctg ttaaagcatg gagcaaaaac acccctacgg cttggcctca tgacgggcat
    21481 cagcacgaaa agggaggcgg cgctcagctc aaggaacaat acgccgacgc tgggttcgat
    21541 atgttgccag atcatgcaac atggcctgat ggaggtaatg cggtcgaacc cgggatagca
    21601 gagatacgcg acatgatgct cgacggtcgt ttcaaggtat ttaacacctg cgagccattc
    21661 tttgaagagt ttcgcctgta tcaccgcgat gagaacggga agatcgtcaa gctaaatgac
    21721 gacatccttt ctgctgttcg ctatggctac atgatgaggc gttttgcaat acagatgcga
    21781 gacatcaaag atcctaaaga gattgattac tcaagctaca acataccttg cggagttgga
    21841 tgatggctga tgatagaaag atggctgact ggcatcgcaa ggtgctgtgc aactttgata
    21901 atgcctggtc agcaacgcag gatatgcgtg agcagattat tgaggctcaa cgtttcgtcc
    21961 gggtgtccgg cgcacagtgg gaaggcagca caaacgctgg ttactcattt gatgaaggca
    22021 ggtttgagca ttacccgcgt tttgaactga ataagattgc ccgtgaatgt gatcgcatca
    22081 ttggcgagta tcgacagaat cgcatcagcg ttaaattcag gccgaaggac gacaaggcat
    22141 cggaagcgtt agccgaaaag atgaacggca aattccgcgc tgactatcag gaaacatccg
    22201 gtggcgaagc gtgtgataac gcatttgatg atgccgtaac gggcggattc ggttgtttcc
    22261 gcatgtgtgc cgattacgaa gatgaaatgg atccgagtaa cgagcagcgc cgcatcagcc
    22321 ttctcccggt ttacgaccca gcgacatgcg tcttcttcga tcaggacagc aagcaatatg
    22381 accgctctga tgctatgtgg gctatggaaa tgttctccat gacgcctaaa gcgttcgagg
    22441 ctgaataccc tgattccatc gcggcaagcc tttttcgtga tgacactggc actcaatatg
    22501 actggtcaac gccagatgcc atctatgttg gacgctacta cgaagttcgc atagagaagg
    22561 tgaagctcac agcatggcgt aaccctgtca gcggagaaac ggcaatctat gatgaagagc
    22621 aaatcaaaga tattgtcgac gagctgaccg atggtgcatt cgaactgatt ggtgagcgga
    22681 cagtgaagaa acgccgagtt tattgcggtc ttctgtctgg cgctgaatgg ctggaagaac
    22741 cgaagcgtat tccgggcgaa catattcctc tcatcccggt atatgggcgt cgttcatttg
    22801 ttgataatca ggagcgaatc gaaggccacg cagcaaaagc gatggatgca cagcgtcttg
    22861 agaacctgat ggtttccatg attgcagata acgccactca ggctggcggt gatggcattc
    22921 ctgtagttga tgttgacatg attcctggtc ctctcgccac tcattgggcg gagcgcaaca
    22981 aaaagcgccc ggcgttcctg ccgatggtca gtctgaaaaa caaaaacgga gatattactg
    23041 cgcaggctca ggtcagcagt tatacgcctc cgacacaaat gcctccagct cttgccgggc
    23101 tattgcagta caccggaacg gctattcagc aaattacagg tgcgtcgcag cttgagaaca
    23161 tgccgagcaa cgtcgccacc gataccgttg atagcatctt taaccggatg gatacgcagt
    23221 cctatatcta catggacaac atggctaaat ccatgcgccg tgctggcgtc gtgtggcttt
    23281 ctatggctcg tgaagtctat ggcagcgata cgccaatgcg catcgttaat gaggatggca
    23341 gcgatgacgt ggcgctgatg actggtgaag tggttgaccg tcagacaggg caggttatcg
    23401 cgcttaacga cctttcgcag ggtaactatg aagtgactgt cgatgtcggt cagtcgttcg
    23461 ctactcgccg tgatgcaacg gttaagtcgt tactttccat gctggcactt atcccgccag
    23521 gaacgccgaa gcatgacctt gtatcgtcga tgattctcga caatatggac ggcgaaggga
    23581 tggacgacct taaagaatac aaccgcaatc agttgcttct gtctggcgtt atcaagccga
    23641 gaacgcctga agaacagcag atggttgaac aggcgaaaca acaacaggcc agtcagccag
    23701 atccggctat ggttgcagcg caaggtcagc ttcttgctgg tcaggctgaa ttgcagaaag
    23761 ctcagaacga acaggcagcc attcaggtta aagcattcca ggcacagact gatgctcagg
    23821 ttgcagcggc aaatgttgtg aaaatcctcg catctgccga tagccagcag aaatctgata
    23881 tccgcgaggc tctgaaactg ctcggacagt tccagcaaca gcaaggagac aatgcccgtg
    23941 ctgatgcaga gcttgtcctg aaaagtcagg cacagggcca tgcgcagcgc atggacatca
    24001 gcagcatcct gcaaaaatca actcagaaac aaccacagca gtaattaacc cataacgtgc
    24061 aatggctgtc tttatgaggc ctggcaacct attgccttcc gatgggctga acatcgagta
    24121 aacaggggta acaaatggac cagatggcag aaaacacacc agaagttgaa atcgaaaccg
    24181 acgcgtcaga gcagattcct gatgatgtcg aactggctga agaagtcgaa acagaagatg
    24241 gcagtgagtc ctccggcaat gatgcagagg aagctactga aactgatgac gacgaatcag
    24301 aacaggaatt ctactttggt gacgaaaagc tggattcgcc aaccagcgaa gatggcgcag
    24361 agcatggact ggtaaaacac ctgcgcaaga cgattaaaga gaaagaccgc gagctgaaag
    24421 agctgatgcg tcagtctcag aaacccgtcg agcagcagcc ggtaatcact caaccaccgc
    24481 gaatgccaaa actggatgat gaggacatcg ggttcgatga agaaatctac cagcaacgca
    24541 tggctaagtg ggcagaggat aacggcaagt accagcaaca ggagatggct cgcaagcaga
    24601 aggagcagga gcttcaggct gcctatcaag agcgattatc caaatatcag caacgtgtta
    24661 aggctctcaa agttcctggc tatcaggaag ctgagcaggc cgtactcgag gaaatcccca
    24721 tcgagacaca aaacgcgatc ctgtttgagt cagagaagcc ggaaatcgtt gttctggcac
    24781 tcggtcgcaa cgctgaactg cgcaagcaac tggcagaagc taccaacccc gtagcaattg
    24841 gtcgtctgct ggaacgtatc gaatcgaagg ccagaatcat gccaaaagca aaaaccacgg
    24901 cagccacaac cccgacagtt aaggggagca acggcgcagt aatcaataac ctcgacaaac
    24961 tgaaagccaa ggcgctggaa actggtgact ggacgccgta tttcgccgct aaaaaggcaa
    25021 aaaaataacc tatcggagca ttaagcatgg ctaaccaatt agcaaaagac cttgaaatca
    25081 tgttcgaaaa ctacgttgaa ggctttgagg ccgcctgcgt agtttcccgt aacgctaaaa
    25141 aattccgtcc cggtgataca gcaatgcagc gagcaggtga tgttctgtat cgtccgcagc
    25201 attaccacat gaacattgag gaaggcctcg acctcagcag caaaacgcca acagcactgg
    25261 ttcagcgcct tgttccttct gtgttcaagg agccgaaaaa cattctgtac actctggatg
    25321 cgcgtgaaat gcgtgacccg gaacataaaa ctgaagctgg tcgcgccgca ggtatgcgcc
    25381 ttgctgcaca gattgactct gacctgattt ccatggtcac gcagcgtgct actaacgtga
    25441 tcacaatggc tgactcaacc actggttcac agggccgtga tttgtggaac tgtgcggcag
    25501 gtattgatgc caccatgacg gcgattggtg taccacaggg tatcaaccgc cgctctttct
    25561 ggaacccctt caactacaaa gaccttgctg gcgagcttgg tcaccgtgcc tatgctcagg
    25621 gcgcaaccct gacagcatac gaaaaagcgc agatccctcc ggttgcgtcc ttcgatagct
    25681 acaagaccga tatttctggt cgtgttccga agggtacagc aacttccctg acgctggcgg
    25741 ctgaacctgc gcacaaggtt gaagcgaaag atgccaacga tatgccagtg gataaccgac
    25801 aggggactat tacggtatct gcatctgggt tgcaggttgg cgatgcgttc accattgctg
    25861 gcgtgaattc tgtacaccag atcaccaaag acaccaccgg gcagccgcag gtattccgcg
    25921 ttctggcagt aagcggaacg acagtaacta tctccccgaa aattctgccg cctgacaacg
    25981 cagatgtcgc cagccgtcca tatgtaaacg ttgatgctaa cgcggcaaat ggtgcagcaa
    26041 ttaccattct caacaagaat gccgcaccgg ctaacctgtt ctgggctgat ggttctgttg
    26101 agctgatgta cggcaaactg gcgttcccga ctggtcaggg tccacaggta atgacagcaa
    26161 ccaccgagca gggcgctacg ctgatcatgt cttacgcctt cgaccacatc aaaggcgtaa
    26221 ccactgctcg tttcaccact ctgtacggtt gctctgtact tgttcctgaa tatacgggca
    26281 tcgttattgc cgggcagtaa ttttggtggg gcttcggccc catttttatt gggagaagac
    26341 aatggcacga acaatgctct ataagcctgg caacatgatc acctgtggtc agtttgctgt
    26401 cgattacatc attgttgatg acgaagaagt taaatctcac ctgaaaaaag gctgggtaaa
    26461 aactcctgaa gaaaccgcaa cgaagcataa agtggctaag gcggaagaag atggcgaaaa
    26521 cgaagggtga tctcgttctt aaggctttac gaaaagccgg gctgtattcc aatgccacgt
    26581 tgacagatgc tgaccctcag gcaattgaag atgccattaa tgacctcgaa gacatgatgg
    26641 cagcatggca ggctaaaggt atcgagcttg ggtatcagtt tgctgataca gaaaacggca
    26701 tcatgccgtt accggacgat gattcaggta tccctgcatg ggcaaatgat ggcgtcgctt
    26761 tgaaactcgc tgtgcaagtg tgcatggata acgtcattca gccgtcagac gctctcctta
    26821 ccgctgctga cagtgcatat cagacaatct gcatcgcttt aaccaaaata ccaccacttg
    26881 agcggcgaaa tgacatgcct cgcggtagtg gtaacaaaag cgcgtttacg tggaatcggt
    26941 tttacatcga gaaagatgat ccgagtacgt gaggtgaata aatgccgatt cagcaacttc
    27001 cgcttatgaa aggtgtcggc aaagactttc gaaacgccga ctatatcgac tatctgccag
    27061 tgaatatgct ggctacaccc aaagaaatcc tgaacagcag cggatatctt cgctcattcc
    27121 cgggcattgc caaacgttct gatgtgaatg gtgtatctcg cggtgtcgag tacaacatgg
    27181 cgcagagtgc tgtttatcgc gtgtgtggtg ggaagctcta caaaggcgaa agcgaagtcg
    27241 gtgacgtcgc tggaagtggt cgcgtatcaa tggcgcatgg tcggacatct caggctgtag
    27301 gcgttaatgg tcaactggtc gagtatcgct atgatggcac ggttaaaacc gtctcaaact
    27361 ggcctacaga cagcggattc acacagtatg agttaggctc agtccgcgat attacgcgct
    27421 tgcgtgggcg ttatgcgtgg tcaaaagacg gtactgattc atggtttatc actgaccttg
    27481 aagacgaatc acatcctgac cgttacagcg cacaatatcg tgccgagtct cagcctgacg
    27541 gcatcatcgg cataggaaca tggcgagact tcatcgtctg ctttggttca tcgacgattg
    27601 aatatttctc cctgactggg gcaaccaccg ttggtgccgc tttgtatgtc gcacagccat
    27661 cgctgatggt gcagaaaggt attgccggga cttactgcaa aacaccattc gctgattctt
    27721 atgcgttcat cagcaatccg gcaacaggtg cgccgtctgt gtatatcatc ggctccggtc
    27781 aggtatcacc aatcgccagc gcgagcattg agaaaatcct tcgttcctac actgctgatg
    27841 aactggctga tggtgtgatg gaatcgctgc gatttgatgc tcatgagttg ctgattatcc
    27901 atcttccgcg ccatgtcctc gtatacgacg catcttcaag cgccaatggt ccacaatggt
    27961 gtgtactgaa aacaggcctg tatgacgatg tgtaccgcgc tatcgacttc atttacgaag
    28021 acaatcagat aacgtgcggc gataagctgg aatcggttac cggcaaattg cagttcgata
    28081 tcagcagcca gtacgacaag caacaggaac acctgctgtt tactccgttg ttcaaagcgg
    28141 ataacgccag agttttcgac cttgaagttg aatcgtcaac tggagttgcg cagtatgctg
    28201 accgcctttt tctctctgca accactgacg gcataaatta cgggcgtgag cagatgattg
    28261 agcagaatga accgttcgtt tacgacaaac gcgttttgtg gaagcgtgtc gggcgaatca
    28321 ggaaaaatgt cggcttcaaa ttgcgcgtta tcacgaagtc acctgtcact ctgtctggct
    28381 gccagataag gatcgagtaa tggcggattc gaatctcaat acacctgtta ttgttcaggc
    28441 gacgcggctc gatacatcaa tccttccacg caatattttc tcgcagtcat atctgcttta
    28501 cgttattgca cagggggctg atgttggtaa tgtggcgaac aaggccaacg aagcagggca
    28561 gggtgcttat gatgcacaag tcaggaacga tgagcaggat gtgattctgg tcgatcacga
    28621 aattcgactg gcatcggctg aagcgaagat acaggaccac gaaacaagga tcactaacgc
    28681 agaagcggcg atagtcggcc ttgattctcg attaacgaca gcagaaaacg atattgatta
    28741 tctgacggat gaagttgtct ccattcaaaa cacgctttca gaccatgaaa cgcgcatcga
    28801 tgctctggag tatgccacta ctcgcaagaa gtcagaggtt gtttactctg gcgtatctgt
    28861 aaccatcccg acagcgccga ccaaccttgt tagcctgctg aaaacgctca cgccgtcatc
    28921 cggcacgttg gcaccattct tcgacaccgt taacaacaag atggttgtgt tcaacgagga
    28981 caaaaccttg ttcttcaagc tgtcgattgt cgggacgtgg cccagcggaa ccgccaacag
    29041 gtcaatgcag ctaacctttt ccggctctgt tcctgacaca ctggtaagca gtcgcaactc
    29101 ggcgacaacg accga
//
LOCUS       DAJTMX010000039        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTMX010000039 DAJTMX010000000
VERSION     DAJTMX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.039"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO145_001749"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO145_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5466893.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO145_001750"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO145_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5466894.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO145_001751"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO145_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5466895.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO145_001752"
     CDS             4843..5082
                     /locus_tag="OO145_001752"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466896.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO145_001753"
     CDS             5092..5262
                     /locus_tag="OO145_001753"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5466897.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO145_001754"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO145_001754"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5466898.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO145_001755"
     CDS             6724..6990
                     /locus_tag="OO145_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5466899.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO145_001756"
     CDS             7084..7773
                     /locus_tag="OO145_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5466900.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO145_001757"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO145_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5466901.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO145_001758"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO145_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5466902.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO145_001759"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO145_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5466903.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO145_001760"
     CDS             complement(10475..12193)
                     /locus_tag="OO145_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5466904.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO145_001761"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO145_001761"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5466905.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO145_001762"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO145_001762"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5466906.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO145_001763"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO145_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5466907.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO145_001764"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO145_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5466908.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO145_001765"
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO145_001765"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5466909.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18432..18746
                     /locus_tag="OO145_001766"
     CDS             18432..18746
                     /locus_tag="OO145_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5466910.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO145_001767"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO145_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5466911.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO145_001768"
     CDS             20570..21862
                     /locus_tag="OO145_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5466912.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO145_001769"
     CDS             complement(22016..23665)
                     /locus_tag="OO145_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5466913.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO145_001770"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO145_001770"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5466914.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVLGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO145_001771"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO145_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5466915.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO145_001772"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO145_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5466916.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO145_001773"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO145_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO145_001774"
     CDS             28753..29034
                     /locus_tag="OO145_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5466917.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7777 c         7348 g         6630 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttattt aatctgcaat
     9421 aatactgatt agtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcaccag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcatcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaagg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTMX010000040        28914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTMX010000040 DAJTMX010000000
VERSION     DAJTMX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.040"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO145_001775"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO145_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5466918.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO145_001776"
     CDS             complement(2138..2584)
                     /locus_tag="OO145_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5466919.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO145_001777"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO145_001777"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5466920.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO145_001778"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO145_001778"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5466921.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO145_001779"
     CDS             complement(5347..6009)
                     /locus_tag="OO145_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5466922.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO145_001780"
     CDS             6182..6595
                     /locus_tag="OO145_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5466923.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO145_001781"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO145_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5466924.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO145_001782"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO145_001782"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5466925.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO145_001783"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO145_001783"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5466926.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO145_001784"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO145_001784"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5466927.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO145_001785"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO145_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5466928.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO145_001786"
     tRNA            complement(9861..9948)
                     /locus_tag="OO145_001786"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO145_001787"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO145_001787"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5466929.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO145_001788"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO145_001788"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5466930.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO145_001789"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO145_001789"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5466931.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO145_001790"
     CDS             14005..14592
                     /locus_tag="OO145_001790"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5466932.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO145_001791"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO145_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5466933.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO145_001792"
     CDS             14984..15841
                     /locus_tag="OO145_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5466934.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO145_001793"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO145_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5466935.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO145_001794"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO145_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5466936.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO145_001795"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO145_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5466937.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO145_001796"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO145_001796"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5466938.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO145_001797"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO145_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO145_001798"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO145_001798"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5466939.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO145_001799"
     CDS             complement(22884..24023)
                     /locus_tag="OO145_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5466940.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO145_001800"
     CDS             complement(24069..26165)
                     /locus_tag="OO145_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5466941.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO145_001801"
     CDS             complement(26165..26911)
                     /locus_tag="OO145_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5466942.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO145_001802"
     CDS             complement(26908..27552)
                     /locus_tag="OO145_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5466943.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO145_001803"
     CDS             complement(27659..27964)
                     /locus_tag="OO145_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466944.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO145_001804"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO145_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5466945.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7321 c         7601 g         6989 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttagcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAJTMX010000041        28672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTMX010000041 DAJTMX010000000
VERSION     DAJTMX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.041"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..651
                     /locus_tag="OO145_001805"
                     /pseudo
     CDS             1..651
                     /locus_tag="OO145_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            648..1034
                     /locus_tag="OO145_001806"
     CDS             648..1034
                     /locus_tag="OO145_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466946.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1059..3272
                     /gene="tssI"
                     /locus_tag="OO145_001807"
     CDS             1059..3272
                     /gene="tssI"
                     /locus_tag="OO145_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5466947.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3295..3740
                     /locus_tag="OO145_001808"
                     /pseudo
     CDS             3295..3740
                     /locus_tag="OO145_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3762..4169
                     /locus_tag="OO145_001809"
     CDS             3762..4169
                     /locus_tag="OO145_001809"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5466948.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4166..6454
                     /locus_tag="OO145_001810"
     CDS             4166..6454
                     /locus_tag="OO145_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5466949.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGVLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6451..7440
                     /locus_tag="OO145_001811"
     CDS             6451..7440
                     /locus_tag="OO145_001811"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466950.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7540..7743
                     /locus_tag="OO145_001812"
     CDS             7540..7743
                     /locus_tag="OO145_001812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466951.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7761..8081
                     /locus_tag="OO145_001813"
     CDS             7761..8081
                     /locus_tag="OO145_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5466952.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8147..8379)
                     /locus_tag="OO145_001814"
                     /pseudo
     CDS             complement(8147..8379)
                     /locus_tag="OO145_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8489..9259)
                     /gene="yafV"
                     /locus_tag="OO145_001815"
     CDS             complement(8489..9259)
                     /gene="yafV"
                     /locus_tag="OO145_001815"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5466953.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9440..9886
                     /gene="ivy"
                     /locus_tag="OO145_001816"
     CDS             9440..9886
                     /gene="ivy"
                     /locus_tag="OO145_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5466954.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9929..12373)
                     /gene="fadE"
                     /locus_tag="OO145_001817"
     CDS             complement(9929..12373)
                     /gene="fadE"
                     /locus_tag="OO145_001817"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5466955.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12613..13191
                     /gene="lpcA"
                     /locus_tag="OO145_001818"
     CDS             12613..13191
                     /gene="lpcA"
                     /locus_tag="OO145_001818"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5466956.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13397..14164
                     /locus_tag="OO145_001819"
     CDS             13397..14164
                     /locus_tag="OO145_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5466957.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14135..14875)
                     /locus_tag="OO145_001820"
     CDS             complement(14135..14875)
                     /locus_tag="OO145_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5466958.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15259..15780
                     /locus_tag="OO145_001821"
                     /pseudo
     CDS             15259..15780
                     /locus_tag="OO145_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15956..16453
                     /gene="rayT"
                     /locus_tag="OO145_001822"
     CDS             15956..16453
                     /gene="rayT"
                     /locus_tag="OO145_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5466959.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16670..18382)
                     /locus_tag="OO145_001823"
                     /pseudo
     CDS             complement(16670..18382)
                     /locus_tag="OO145_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18354..19139
                     /gene="lafU"
                     /locus_tag="OO145_001824"
     CDS             18354..19139
                     /gene="lafU"
                     /locus_tag="OO145_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5466960.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19210..20265
                     /gene="dinB"
                     /locus_tag="OO145_001825"
     CDS             19210..20265
                     /gene="dinB"
                     /locus_tag="OO145_001825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5466961.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20317..20610
                     /gene="yafN"
                     /locus_tag="OO145_001826"
     CDS             20317..20610
                     /gene="yafN"
                     /locus_tag="OO145_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5466962.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20613..21011
                     /gene="yafO"
                     /locus_tag="OO145_001827"
     CDS             20613..21011
                     /gene="yafO"
                     /locus_tag="OO145_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5466963.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21021..21473
                     /locus_tag="OO145_001828"
     CDS             21021..21473
                     /locus_tag="OO145_001828"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5466964.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21719..22762
                     /locus_tag="OO145_001829"
     CDS             21719..22762
                     /locus_tag="OO145_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5466965.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22824..23438
                     /locus_tag="OO145_001830"
                     /pseudo
     CDS             22824..23438
                     /locus_tag="OO145_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23495..24952)
                     /gene="pepD"
                     /locus_tag="OO145_001831"
     CDS             complement(23495..24952)
                     /gene="pepD"
                     /locus_tag="OO145_001831"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5466966.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25214..25672
                     /gene="gpt"
                     /locus_tag="OO145_001832"
     CDS             25214..25672
                     /gene="gpt"
                     /locus_tag="OO145_001832"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5466967.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25764..27008
                     /gene="frsA"
                     /locus_tag="OO145_001833"
     CDS             25764..27008
                     /gene="frsA"
                     /locus_tag="OO145_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5466968.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27066..27467
                     /gene="crl"
                     /locus_tag="OO145_001834"
     CDS             27066..27467
                     /gene="crl"
                     /locus_tag="OO145_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5466969.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27506..28561)
                     /gene="phoE"
                     /locus_tag="OO145_001835"
     CDS             complement(27506..28561)
                     /gene="phoE"
                     /locus_tag="OO145_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5466970.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7083 a         7125 c         7606 g         6858 t
ORIGIN      
        1 aaaacaaata ttgatggcaa tatcattata cataaggatg gtaggaggac gtatttatta
       61 aatcaaaata ctggttcgat tacggctgtg ataagatgga agtatgagta gtataaggat
      121 aataccgtta cagatgactg gactatatgg gaaaaagcta tttttatgcg agcgatatat
      181 ttatctgttc agcaggcatg gaatgggaaa ataacttatt ctgtttccgg tgaatctgag
      241 ttcgctaaaa aatttcaggg taaagctttg ccttttgatg taagaataat ttctgcatct
      301 cagaatgaag actggttggt tattgcaaca aaggttttgc ctggtgctga tttaagaaca
      361 tatgttgatt ttaaaaacag tacggttcat gttgattcag cgggcctgga aaaagtagcg
      421 aaatgtatta actgtaataa tacgttgcaa gtaaatatac ctcatgaagc cggacatgta
      481 cttggttact tagatgatga ttatgattct tcgtctccgt atgttggaga tattagtggg
      541 ttgatgaatg tgggaatgga gctttgggaa cgatacctta aaaacgcaac tataacgttg
      601 aatatcataa tgcctgaaac taaatttact ttattgaatg taaccaaatg aaatttttct
      661 gtattatagt gattttcctt tcaataaatt acatagctat agtgaatgtt ttcaagtctt
      721 ctgcagttgc tcaatccacc aaatattatg aagaaaatga tatacatgat ggaagtaatg
      781 acatcttttt tatgaagaaa tatgggccgc ctgctcatga gttcgtgagg ccacgatata
      841 aatttgttgg tgagtattat atcggcttga atcaaactta tcgcataaca gatcctgaat
      901 ttcaagatgt tcctattaag gaaatgttct ggaatttgca gggggatttg aatctgacat
      961 gttggtttca ttataaagac gggcaatgga gagtgattac ttatattttt tggccaccag
     1021 gggctagatt ttaatccggt attaaaggag caaatatcat gagttttgta tctacgggta
     1081 ataaaccagt tggaaacggt ggacccgtca tcaccacacc accaatagct ggcgaaagtg
     1141 gcggtatgag cacaggttca gctgtaaccg atgtatctgg tgccgccgag gagatggcag
     1201 aacaggctgc ggcggacctg tttggtgcgc tgccggaacc gtcaggtctg gttaaagccg
     1261 cagtagctgc tgcgcaggct gccgctgcag cagcagggat ctcggatatg gctggtgcag
     1321 ttcaggatgc agcggcgtcg cttgcagccg gtgctccggg ggcgcataac gtcacagtaa
     1381 gtggtagtgc ggtgccgccg cagatgcttt tgtttgcagg catgaatggt tcggaagggc
     1441 ttgggaatct gttttcttac acagtacagc ttaaaacgct ggacgccctg aacctggggt
     1501 acgtttcccc cgctgctaac ctgccgcttc agctcatggt gggcaaagac ctgtgcgtca
     1561 gtatcgaact ggacggcggc ggaaaacggt atatcagcgg gctggtgacg gcggcgaggg
     1621 tggtcggcca tgagagccgt tcggtaacgt atgaattacg cattgagcca tggctgaagc
     1681 tgctgaccca taccagcgac tacaaagcgt tccagaacaa aaccgtggtg gatattctcg
     1741 atgaagtact ggatgaatat tcctttccgg tggaaaagcg gctggtggaa aattatccac
     1801 cccgcgcctg gcaggtgcag tatggtgaaa ccgacttcga tttcatccag cgcctgatgc
     1861 aggaatgggg gatttactgg tggtttgagc acagcgagaa cagccacacg ctggtgctgg
     1921 tggatgccat caatggtcat aaagcgtgcc ctgactcgcc gctggtggag tggcaccagg
     1981 aagggctgaa actggataag gaatttatcc acaccattac ggccagtgag aggctgcgta
     2041 ctggaaagtg ggtgatggac gattttgatt ttatgaagcc tcgttcgctg cttaaaagta
     2101 ccgtggccag tccgcgtgat accggtcatg ctgaatacga gcattacgaa tggcctggtg
     2161 attacttcac tacgggcgaa ggcgagatgc tgacgcgcat tcgtatggaa gcgcagcgca
     2221 gcccaggcag ccgggcacat ggggccggtc atatccgtac cctcatgacc gggtacacct
     2281 ttacgttgat gaatcacccg acggcagaaa ttaatcagga atatctgctg gtacagacca
     2341 ctctgttttt acgggataat gcgcagcaca gcggtcagga tcagcatttt acctatgtca
     2401 ccacgtttga actgcacccc accagcgaag tttaccgccc acaacgaacc ctgagcaaac
     2461 cccacaccaa aggcccgcag agcgccatcg tcaccggccc ggcggggcag gaaatctgga
     2521 cggataaata tggccgggtg aaggtgcagt ttggctggga ccgttacgga aagaatgacg
     2581 aaaacagttc ctgctggatc cgcgtcagct atccgtgggc gggtaaagga ttcggcatga
     2641 tccagatccc gcgtattggc caggaagtac tggtggattt caaaaacggc gatccggatt
     2701 tgcccatcat cgtggggcgc acctataacc aggacaccat gccgccgtgg gggctgccgg
     2761 gcatggcgtc gcagagtggg atattcagcc actcattgca gggagggccg actaacggaa
     2821 atatgctgcg cttcgatgat aaaacgggtg cggaagaggt gaagttccat gcggaaaaag
     2881 acctcaatac cacggtaaaa aacaatgaaa cccacacggt catggtcgat cgcataaaaa
     2941 ccattgtcaa aaatgaaacc atcagcgtgg gagaagaccg taatgccacc atcacaaaaa
     3001 atgatggtct ttccgtcaaa ctggctcaaa ccatcaatgt cggaacgaca tatcgtctgg
     3061 atgttggcga tcaatttacc cttcgttgcg gcaatgcagc gcttgtatta cacaaggatg
     3121 gctcgattga gttttgcgga aaacaactgt tactgcacac cagcgatgtt atgcaactca
     3181 ttggcagtgg cattgatatg aatccggatg gcggcacagc catgactgcg gatgatattg
     3241 ccccgttacc tacccccgac gtttctgaat aaaaaatata aaaggaattt ttctatgacc
     3301 agcctttacc gtattcagga gggctgcttt gccctgcctg aaacttttct tgatcgcacc
     3361 gtcaatatct tcgtcccatc gggcaatgaa agggcaactc ccagtctgaa tattttccgc
     3421 gatacgctca ggccagatga aaatctgacc acctatattg accgccagat agccttgatg
     3481 aaaaaaaaaa cctaggccag caccgggtac tttcgcgggg aacggcactg gcgggaactg
     3541 gcgaagctgc catgacaggt gaacagattc tcgcaactca tcgttctgga aaaacagaag
     3601 tgtaccagcg tcaggccggg tttatcaccg ggccagcaaa ggtactaatg ttgacgctga
     3661 ccactcagcg tccttttgat gatcacaccg accagctatg gactgcatgg ttgactagtt
     3721 ttcagcctgc taaaagctaa aaaaaacagg gaggttttgt catgtatgaa gccgcacggg
     3781 tggatgaccc catctaccac accagcgccc ttgccgggtt tttgataggg gccatcatag
     3841 gtatcgccat tattgccgtc gccagctttg ctttttttac ctgcggcttt ttggccgggc
     3901 tgattttgag ttttctggct gatcagatag cttccggagt cctgcaactg ggtgaggcta
     3961 ttgggcgctc gatacattcc acagtgggaa aaattctgac cggttctgat aatgtcagca
     4021 ccaacagtcg cccggccgcc cgggccgtac tgagtacggt gatatgcgaa gaccattccc
     4081 cggaaatccg cattgcccaa ggttccggca acatctacat caacagccag ctgcccgtaa
     4141 ggatgaccac actgagtgtg atgcagtgat tgaggacggc tcccccaatg tctttctcgg
     4201 cggcggtacg cagacggtac tggaaatcag cccggaaatc cctgactggc tgcgtcaggt
     4261 ggtggatgtg ctgtatgtgg tcgccggaat gcttggcggg ctggctggcg cctggcgtca
     4321 ggcggcaaag atggggtcga aatttggtac aaaatgtgcg gcaaaattta ttggtggcga
     4381 aatggtcggg atggccatct ctgacacagt catgggattg ttcagcaatc cggtagatgt
     4441 caccactggg caaaaaattt tgctgccgga gacggatttt accctgccag gacgcctgcc
     4501 tgtcacctgt tcgcgctttt acgccagcca cctggaaact gaaggattgc tgggcagagg
     4561 atggcggctg aactgggaga taaacctgcg tgaggatgaa acatatatca ccttcatcgg
     4621 tgtacagggt cgggaactga gttatccgaa agagatgctg ataccgggcc accagatatt
     4681 cgacccggaa gagcagtttt acctgagccg tctgcatgac ggatgctatg tgctgcatta
     4741 caccgactgc agctattacg tgtttgatga atttgatgac cacggcgtcg cgccgttact
     4801 ttttatggag acgccgtacc ggcagcgtat tgctttcggg cgtgagaacg gcaggctggt
     4861 gcgggtggcc tccagcagcg gacaccatct gttattgcac cgcacgatga cgcaagcagg
     4921 ggaacgcctg tcgcatattg aacttttgaa gggcgggcgt cccggtaacc tggtggaata
     4981 tcgttacgac gataacgggc aactgactgg cgtggtgaac cgggctggtg tgacggtgcg
     5041 ccagtttgcc tatgaaaatg gcctgatgac agagcatcgc aacgcgacgg gctttacctg
     5101 tacctaccac tgggaagaga ttgaaggttt cccgcgcgtg gtggaacaca cgaccagcga
     5161 cggcgaggat taccggttcc actatgattt tgctggcggg cagacggtgg taaccggcag
     5221 gccggagcag aagtggcagt ggtggtttga tgaagagacg tatgtgactg cgcaccggac
     5281 gccgggcggc ggaatgtacc gcttcacgta caacgaaaac cattttccgg tagcggtgga
     5341 actccccggc gaacgcaggg taacgctgga atacgacacc ctgagccggg tggtgaaaga
     5401 gacggacccg gcaggccggg tgacacagac gcagtggaac ggcagttttg ctgaaatcac
     5461 gcgccgggcg ctggatgacg accacgtatg gaaggcggat tacaacgaac acggccaggt
     5521 gatacgggag acggacccgg aagggcgcgt cacgcggtac gggtatgatg atcaggggct
     5581 gccggagacg gtctgccacc ccggtaaaca gcaggaccgt tacacctgga acgcgctggg
     5641 ggtactgagc agccaccgtc gcatcacggg cagtgtgcag tcctggcagt atacgcagcg
     5701 cgggatgctg gcgcgccata cggatgaaga gaaacgggag acgcgctggc agtacacccc
     5761 ggaagggctg gttgccagtc tgagcaacgg caacggggct cagtaccggt tcagctacga
     5821 cggcgacgga cggctgacgg gagaacagcg cccggacggc ctgataagga tgtttgcgct
     5881 gaatgcggac gggtttccgg tcatcatccc gacgcagggc acagagggcg gggtgcgcaa
     5941 tgaacagcag gagcgtgatg cgctgggccg cctgctgcgc agtgacacgc agcacagtac
     6001 acggaccttc agctacaacc gtcttgacca gataacggaa gtgacgctca cccccacgga
     6061 agaaggggaa cggctgcacc acatgcaggc ggacacggtg cgttttgcgt atgaccgcag
     6121 cgggtggctg acggcagagc acagcgtgca cggcagtatc aaataccggc gcgatgcgct
     6181 gggcaatccc accgatatca cgttgccgga cgggcagcat ctcagtcatc tttactacgg
     6241 cagcgggcat ctgctccaga cggcactgga cggtatcacg gtcagtgaat atgagcgtga
     6301 cagcctgcac cgtcaggtga tacgcacgca ggggaaactg gcgacgttca gcggctacaa
     6361 cgccgacaac cggttaagct ggcagcgcag cctgccaggt ggcagccaga atccacagca
     6421 ggcggtactc ccccggcgca gaacgacagc gtgacggcgc gggactatat ctggaacgca
     6481 gacggtgaag tcggcggcat caatgacaaa ctgcgcggtt gcctggtgtt cagctatgac
     6541 cgcagcggct ggctgaccag ccgcacgggg cagatgtatg accatgacca ttattattac
     6601 gacaaagcgg gcaacctgct gacggatgag tatcagggag cggtgatgga taaccgcctg
     6661 ccgggttacg ggcgtgaccg ctaccgctat aacgaatggg gcgaactgac agagcgcagg
     6721 gaccagcagc tggagtggaa cgcgcagggc cagttaacgc gggtcatcag cagcaacagc
     6781 gagacgcggt atcagtacga tgcgctgggc aggcgcatca gcaaggcgac cagcaatctc
     6841 cataccgacc ggggagaacg aagccggaca acgtttgtgt gggaagggtt ccggctgtta
     6901 caggagacaa cgtggcaggg aaagcggaca tacctgtacg atgcggaaca gccgtacacg
     6961 ccggtggctg ccatcacggg gcgcggagag agccagaaaa tctggtatta ccacacggac
     7021 ctgacgggca cggtacacga ggtaaccgcg ccggacggga cgctggtgtg ggcgggctac
     7081 caggcgggat ttggtgaaaa ccggggcgac atcagcaaca gcggggcgta ctttgagcag
     7141 ccgcagcggc tgccggggca gtactttgac gaagaaacag ggctgcatta caatctgttc
     7201 aggtattatg cgccggagtg cgggaggttc gtaagccagg acccgatagg attaaacggt
     7261 gggttgaact tatatgctta tgcgcctaat ccgttaggtt ggatcgatcc gctaggactg
     7321 aattctttag gaatagattc aaataatata tttagaggag atggtaacta taaaggtggc
     7381 ggaattggac agccaaagag tggcacaata tcagcacagg acattattga tcatctctaa
     7441 gaaaaaggtg atgggcgttt aacatctttt agtactaaaa aaacatctag ttccaactct
     7501 cgtggtgcag attttttctc aaaaaaccat gtcttcaaag tggcagtttc tgatctcgat
     7561 gatttagtta aaaataatca gatcactgtt ttaacacaag aaaaagtaaa agcaatattt
     7621 ggtttagaat caagcaagaa aattagaaaa gaacttggta atattcttca gaatatggct
     7681 aggaataatg aggtgttagt tcatgggcaa attcttggaa ataaagttag ccgatgtaat
     7741 tagttagtgg agaggaagtt gtggacgaaa ttagaataag aattgctggt aatacggctg
     7801 cgccttgttt ccatgtgcta aaaaataagg gatataaagt aacaatggaa actcatgcat
     7861 tatctgacga ttgtttagat tgtgtatatg attggtatgc ggaaaaaaat ggacaatttt
     7921 ttagtgcaac gagtccagaa gagttacttg gattagttgc aatgtgggaa gttcgtggta
     7981 atgactggag agctaatttg aatgagataa atacttatca aaaagccata gaagaatccc
     8041 cgttttttga taaaaacggc aatgaaatag agtctgacta aattttattg atatagcaac
     8101 gataataacc ggaaatttct tccagatctt tccggctttt tattatttaa ctttcgttca
     8161 gcacactaaa catcccgtct cgcatcccct taactggctg tttaacctga taaagttccc
     8221 tgcgcagttc ctgtacttcg ttgttatccc aagcaggatc aaacatccct ccgagatctt
     8281 cacggtaacg cccgtgccga tctcaaatcc cgcttttctc agccatttgg cgctgatttt
     8341 gatcaccggc gtgtcgctgc ggttgggcag ataacccacg gtacagcgac gctgtgtttt
     8401 ggaaatggtg acttctggcg taatattgtg tttaagttag gggctttcgt aatctagccc
     8461 agcccccggc cctaaacaca acccccactc accacaacct aaactcatcc gcatcccgcc
     8521 atgccggaaa cttttctcta tattcccgca atgccaccat cgacagctcc gcatcaatac
     8581 gcgttgcctt atgcgcgtcg gcggtagcga taatctcgcc ttgcggatta atcacccggc
     8641 tgtcaccgcg ataatggcag ccgttgccat cgctgccgac gcgattgcat cccgccacat
     8701 acgcctgatt ctcaatcgcg cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag
     8761 ctggccagtt ggcgacgtac agggcgaggt cataatcgtt gagattgcgc gaccacactg
     8821 gaaaacgtaa gtcgtagcac accagcggca aaatacgcca gccgcgccat tccacaatca
     8881 ctcgcgcatt gcccgcttta taatgcagat gctcatctgc catgcggaac agatgacgct
     8941 tatcataaaa atgtaccgtg ccgcccggct caaccagcaa aaagcggtta accgaaccag
     9001 actccgtttg taatgcaaca ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg
     9061 tcatccagtt cactacgtca tcttgtgcaa gcgacgaagc tgccgcttcc atggcaaagc
     9121 cgctggtaaa catctccggt agaacgatca catcgcgccc ggtaatacct tccagttgac
     9181 gatcaaaatg gcgcaggttg gcaggaccat ccacccacac caacggttgc tgcaaaagcg
     9241 taatcttcaa actaggcacg gtgtacaact cctttatgcg aagggtttta taactttaac
     9301 accttatcag gcagttgcct tagcgcagaa taaattgata acaaatgctg atattggaaa
     9361 tatctgattt gcaaattatc gtgttatcgc caggctttag gaggttaata acatgggcag
     9421 gataagctcg ggaggaatga tgtttaaggc aataacgaca gtcgccgcac tggtcatcgc
     9481 caccagtgca atggcgcagg atgatttaac cattagcagc cttgcaaagg gcgaaaccac
     9541 caaagctgcg tttaatcaga tggtgcaagg gcataagcta cctgcatggg tgatgaaagg
     9601 cggtacttat actcccgcac aaaccgtaac gttgggagat gagacgtatc aggtgatgag
     9661 cgcgtgcaaa ccgcatgact gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc
     9721 taatcagatg acggggctgt tctcggctat tgatgagaaa acgtcgcaag agaaactcac
     9781 ctggctgaat gtgaacgatg cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt
     9841 gaccggcagc ctggaaaacc atccggatgg ctttaatttt aaataattag cggataaaga
     9901 aacggagcct ttcggctccg ttattcaatt acgcggcttc aactttccgc actttctccg
     9961 gcaactttac cggcttcgtc gccagctctt ccggatcaaa gtcatcaacg ttaatactgc
    10021 gcagacggct ttcttccgct ttcaccagaa tagcggcttc atctttatca atcagcccct
    10081 tcgtcagcgc gttgtgcgcc agttcatcca gacgggtaaa tggcagattt ttacccagct
    10141 ctttacagat ccgctgatga atcgggtcgg cggcaatcac atccaccaac gcctcttcca
    10201 gtaaaccaac cgggttatgc tcgcttggcg tcaggtactg accgcgacca atacgtgaac
    10261 gagtggcgtt cggcacttgt aaaatcttcg ccattttatg atccagcttg tcagacggtg
    10321 ccagataatg acgtccggtc gggaagatca ccacattcag cagcccggca accacgcggt
    10381 tcgggaagtt ttgcagtaaa ttgtccattg cctgttctgc ctgatacagc gcatcctgta
    10441 cgccccagtg caccagcggc agatcggctt cattacggcc ttcgtcgtca taacgcttca
    10501 gaacggcaga ggcgaggtaa agctggctta aaatatcccc cagacgtgcc gagatacgct
    10561 cacgacgttt caggctgccg cccagcagtg ccatcgagac atcagaaagc agggcgaggt
    10621 tggcgctcag gcggttcagg tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc
    10681 tgctggttaa accgcgcgtc aggcccagcc agaagctgcg aactttgttg ctaccgacgt
    10741 gaccgatatg tttgaacaac agtttatcga acgcgttgac gtcattgttc ttcgccgctt
    10801 ccatctcttc cagcacgtac ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc
    10861 tgcgggtcag aatgttagcc ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac
    10921 gcgccaggaa gttgctttgc ccgagcataa tgcctttacc gccggtaata tccatcgcat
    10981 caataatcga ctgctgcccg cggtgggtac agtgatactt aacgatagcc gacagcacgg
    11041 caggtttttc gccgagcata atgccgtagg taatcagcga tgccgcagca tccattacat
    11101 aagcgttccc ggcaatacgc gccagcggct cttcaatccc ttccatctta ccgatagaga
    11161 ttttgaactg acggcgaatg tgagaatacg cgcccgttgc cagcgctacc gatttcacgc
    11221 cgccggttga gttggaaggc agggtgatgc cgcggcctac cgagaggcac tccaccagca
    11281 tccgccagcc ttgcccggcc attttcggcc cgccgatgat gtaatcgatc ggcacgaaga
    11341 catctttacc gcgcgtcggt ccgttctgga acggtacgtt cagcgggaag tggcgacgac
    11401 caatttccac gcccggcgtg gtggttggga tcagcgcaca ggtaatgcct aaatcttctg
    11461 caccgccgag taatttttcc gggtcggaga gtttaaacgc cagcccaagc acggtcgcaa
    11521 tcggtgccag cgtaatgtag cgtttgttcc aggtcagacg catccccagc acctgctggc
    11581 cctgccattc gcccatgcag acaatcccgg tgtccggaat cgcgcccgca tcggaacccg
    11641 cttccgggct ggtcagtgca aagcagggga tctcctgacc acgcgccaga cgcggcagat
    11701 agtgatcttt ctgctcgtca gtgccgtaat gttgcaacag ttcgcccggg cctaatgagt
    11761 ttggcacgcc gacggtaatc gccaggatcc cgctcacgcc agagagtttt tgcagcacac
    11821 gagactgggc ataagctgag aactccagtc cgccgtactc ttttttgata atcatcgcga
    11881 agaagcgatg ctctttaagg tacgcccaca actccggtgg cagatccgcc agctcatgag
    11941 taatctggaa atcattcgcc atccggcagg cttcttctac cgggccgtcg agaaacgctt
    12001 gctcttcagc ggtcaggcgc ggctgcggat agttatgcag ctttttccag tccggcttgc
    12061 cctggaacaa gtcgccctcc caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc
    12121 gcgacatcgg cggcatcacc ttacggaaac cgcgaaatac cggcgcggaa atcatcgact
    12181 tacgcatagg cgcaaagtta aatggcacga ggataatggt cagaggcacc agtacccacg
    12241 ccgaccacag accagcaacg ccgagggcgg ctgtccaggc gagcaaaatc agactgctga
    12301 taaataagct cacgcggtga tagaacaacg cgccgagcag gacaaccgta gcgagaatac
    12361 tcaaaatcat cataacgaaa agccccttac ttgtaggagg tctgaccact tgtgatgata
    12421 tggttgtagt ggatgtaaaa acatttagca atgtgtttac aatataatta caacaaagct
    12481 cacattgttg ctgtttttat ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc
    12541 cgtacttctc gcttttggcg gtatccggta cactgcattt tgtctattac atttatgctg
    12601 aaggatatcc tcatgtacca ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg
    12661 ctggctaact ttttaaaaga tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg
    12721 ttagcagaca gctttaaagc cggtggcaaa gtgctttcct gcggcaacgg cggttcccat
    12781 tgcgacgcta tgcactttgc cgaagagttg accggtcgct accgtgaaaa ccgtccgggc
    12841 tacccggcga ttgctatttc tgacgttagt catatttcct gcgtcggtaa tgatttcggt
    12901 ttcaatgata ttttctcccg ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg
    12961 gggatctcca cctccggtaa ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag
    13021 aaggggatga aagtgatcac cctgaccggt aaagacggcg gtaaaatggc tggcacggcg
    13081 gatatcgaaa ttcgcgttcc gcactttggt tatgccgacc gcattcagga gattcacatt
    13141 aaagtgatcc atatcctgat ccagttaatt gaaaaagaga tggttaagta agtctggcgt
    13201 aggccggata aggcgtttac gccgcatccg gcacttgtgc gctgatgcct gatgcgatgc
    13261 tgacgcgtct tatcatgcct acaaatctgt acccgaaccg taggccgaat aattcgttta
    13321 cgccgcatcc gacctgaaaa ttcttaaatc aatcttcgcc ggggtccatg cgctcccgct
    13381 gttgtggagg ttacccatgt gcgaattgct cgggatgagc gccaacgtcc ctaccgatat
    13441 ctgctttagt ttcaccggac ttgtacagcg tggtggtggt accgggccac ataaagatgg
    13501 ctggggcatt accttttacg aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag
    13561 ctttaattcc cccatcgcca aacttgtcca ggactacccg ataaaatcct gttcggtggt
    13621 ggctcatatt cgccaggcta atcggggcga ggtggcgctg gaaaatactc acccatttac
    13681 ccgcgagtta tgggggcgta actggactta tgcccataac ggacaactga cgggctacaa
    13741 atcactggaa accggcaact tccgcccggt cggtgaaacc gacagcgaaa aagccttttg
    13801 ctggctgctg cataaattaa cgcagcgtta cccgcgcacg ccgggcaaca tggcggcagt
    13861 gtttaaatat atcgcctcac tggcggatga actgcgggcg aagggcgttt tcaacatgct
    13921 gctttcggac gggcgctatg taatggcgta ttgctcgact aatttacact ggatcacccg
    13981 ccgcgcgccg tttggcgtgg caacgttgct ggatcaggat gtggaaatcg acttcagctc
    14041 gcagaccaca ccgaatgatg tggtcacggt gattgcgaca cagccgctga cgggcaatga
    14101 aacctggcaa aaaattatgc caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt
    14161 ttgatgccag ttgtggctgc accacttcgt ggcttaacgg cttgctgacc acgtaacggc
    14221 cattggccac agaaacggtc ggtggcttac gggtttgctc aaagtagtcg tagcccggct
    14281 tcagctgctc ccagaagtcc ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg
    14341 tcatgcggaa tgggtaaata ctcacctgca cgctcggctg accaaacacc agcgcaccag
    14401 taacgaactg gaagatctca tcaataccct gattggtcat tgcgtagcag ccgatggata
    14461 cacaatcgcc gtggatcatc aggtatttcc cttcataacc atgcgcacgg tcataagcat
    14521 tggggaatcc aatattaatc gctttgtagt aacggctgtc tggttttaac tgattacgct
    14581 ggacgctata aaacccttcc gggcttttga aatcgccctg acgctgtttt ggccctaagc
    14641 cgccggaata tttacagatt ttataactgt cgagcagttg atattgctcg cccattttga
    14701 cgtaaagatc gagcgtacgt tcttccttga agatctggat gtagacaggg gatcccatca
    14761 actgctgctt atactctttg ctcactggcg tggtcgagct actgctgccc agcaaaccgg
    14821 caaacgaaac gcacgggatc aacaacatcg caagaattaa tgcgatttta cgcatactgc
    14881 ttattccttg ataaaacggt tacacacgcc aggacggcaa aatggatccc aaatcggaat
    14941 agtctggatt tggaaggctc acattatcac caaaagagtt ttacgcaagc ctgtcggcgc
    15001 tgggtttaca aatttagcac aggggcagtt ttcaatagtc cgaaaagcgc atgtatcatt
    15061 tatttcctaa tattatgaaa tctcaatagt aatatgcaaa taagcaatta ataatcccgt
    15121 ttactcctcc ggtcttttaa aattctcacc ttcattcagc cggtttatgt ctttcatgtc
    15181 ttcgttcttg ctcggcagat ttttgcatcc aggcgtgttt tcgttatgcg ttttgctccc
    15241 tttactcgcc agcgccacca caaccgggag cagataaaac agcatccaga atggtttcct
    15301 gcgccgctca aggcgagtga cagacgctgg caggcgctgg cggaaaacaa ccacttttta
    15361 agcagcgatc acctgcataa catcaccgaa gtggcgattc accgcctgga gcagcagctt
    15421 ggcaagcctt acttctgggg cggcacgcgg cccgatcaag gcttcgattg tagtgggtta
    15481 gttttttatg cctacaacaa gatccttgag gcgaaactcc cgcgcacggc caatgagatg
    15541 taccactacc gtcgggcaac gattgtggcg aataacgacc tgcgccgggg agatttgcta
    15601 tttttccata tccacagccg cgagattgcc gaccatatgg gggtgtatct gggcgacggt
    15661 cagtttattg agtcactgcg caccggggaa accattcggg taagccggtt agccgaacct
    15721 ttctggcagg accatttttt aggcgcgcgc aggattttga cggaagagat gattttgtag
    15781 gccggataag gcgtttacgc cgcatccggc ggttgtgcac ggatgcctga tgcgacgctg
    15841 acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc cgatataaca
    15901 cagcgtcatt gtcggccttt ccttttcctc ttaccgttga gaaaaaggag tcgctatgtc
    15961 ggaatatcgt cgttattaca tcaagggggg aacatggttt ttcacggtaa atttacgcaa
    16021 tcgtcgaagc catcttctga ccacccagtt ccagacgctc cgtaacgcca ttattaacgt
    16081 taagcgagac agaccttttg aaattaacgc ctgggtcgtt ttgccggagc atatgcattg
    16141 catatggaca ttacccgaaa gcgatgatga tttttcctct cgctggcggg agattaaaaa
    16201 gcagttcacc catgcttgcg gattgaaaaa tatctggcaa ccgcgttttt gggagcatgc
    16261 cattcgcaat acaaaagatt atcgccatca tgttgattat atttatataa atccagagaa
    16321 acatggctgg gtaaagcagg tgagtgattg gccattctca acgttccatc gcgatgtcgc
    16381 gcgagggtta tatcccatcg attgggcggg agacataacg gatttaagtg ccggggagcg
    16441 tatcatttta taactgtgcg ctggtgccgg atgcgcttac gcttatccgg cctacaaatc
    16501 gtgctttaac ggtaggccgg ataaggcgtt cacgccgcat ccggcagtta tgcgcagatg
    16561 cctgatgcga cgctggcgcg tcttatcatg cctaaaaact tgtgcctgaa cggtaggccg
    16621 gataaggcgt ttacgccgca tccggcaatt gtgcaccgat gccggattct tacatcaacg
    16681 cccccataat cttcaactcc agctcatccg gcacttcgtt atacgacagc acatgcagcc
    16741 ccggcgcaaa caaccttgca taacgcggca gcaaagggcg tagctgcggc ggcaccagca
    16801 gcaccggatc tttccccgcc gctttcatct gctctttcac ctgtggcatc gtgctctgga
    16861 actggttgag catattcggg tcaaccggca cgctgtcgag catcactttg ccggcctgtt
    16921 gcgcctgatt cactacgttg gtcagcagat tttccagctc attattcagc gtatacaccg
    16981 tcagctcctg cttgcgaacg aacggatgag taatgctgcg ccgcagcgcc aggcgcacat
    17041 cggccgccag cagaatatga tctttagtca ccgtgctact ggcgaccagc acggtggcga
    17101 tggtgacgat atcgcgcagg gaaacgcctt cggtcagcag cgcccggtac actttcagca
    17161 attggctgta attgagcgcc gcgcttaaat cctccgccag gcgcggtgcc atcgacgaca
    17221 aacggttatg caactgcgtg atgtcatcat agttaaacaa atcaggaata tagctgcgca
    17281 caatcttgtt cacatgggtg gcaatcacac tggcgctgtc gatcacctga taccccatat
    17341 tcagagcctt cgctttctgc gcaggctgga tccaggtgac cggtatcccg tacgccgggt
    17401 cgttccccag cacgccgtca atctcgccgt aggtttcgct ggagggcagc gccatcagtt
    17461 tatccgccgg aatatccgct tcatcagcct caatgccgtt gatgaaaata gcgtactggc
    17521 tgggcttgag gcggaagttt tcccgaatgc ggatctccgg cagcagcacg ccgttaccgt
    17581 cggaaatcac ctgccgaacg ccgcgaatcc gctgggtgag cgggttgccc tgcgctttgt
    17641 ccaccagcgc caccagcttg tagccgaggc ttaagctgat gggctcgatc agcggaatag
    17701 tttcccaact gacctgttgt tcgctggttt cagtgatggt gcgggtcagc gtttcgaggc
    17761 ttttctcttc cgcctccgcc gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa
    17821 gcagggcgct gaacagcaaa aacggcagat gcggcatccc cggcaccacc gccagcacga
    17881 acatgatccc ggtggcggtg tagagcaccg acgggcttgc cagcaactgg tggcgcacgt
    17941 catgggcgat atcgccgttg tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga
    18001 gcagcaggga agggatctgc gccaccaggc cgtcgccgat ggtcatcagc acgtattgct
    18061 ggaaggcggc gtcggcgctc aggttgtatt tgaagatgcc gatacagacg ccgccgctca
    18121 ggttgatcgc cagaatcatc atcccggcga tggcgtctcc gcgtacaaac ttcgatgccc
    18181 cgtccatcgc gccgtagaaa tcggcctcgc tggcaacatc tttacgccgg gtttgcgcct
    18241 gcgcctggtt gatcaatccg gcgttaagat cggcgtcaat cgccatctgt ttgccgggca
    18301 tcgcgtccag ggtaaagcgg gcagaaacct cggaaatacg ctcggcctct ttggtgacga
    18361 cgataaagtt gattgtcaac agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc
    18421 tgcacgggca gtcgattttt aacggtggtg ggctgtcgcc gttaaataaa atcagcccgt
    18481 ctcatccgcc aaaacctgcc accgtggcgg tgccggaaga gacggagaaa aaagcgcgtg
    18541 atgtgaatga gaaaacggcg ctgctgaaga agaaatccgc caccgagctt ggcgagttgg
    18601 caaccagtat caacaccatt gcccgtgatg cgcatatgga agcgaatctg gagatggaga
    18661 ttgttcccca gggattacgc gtgctgatta aagacgacca gaatcgcaat atgttcgaac
    18721 gcggcagcgc gcagattatg ccgttcttta aaactttgct ggtggagctc gcgccagtgt
    18781 tcgactcgct ctataacaaa attattatta ccgggcatac cgatgcgatg gcctacaaaa
    18841 acaatatcta caacaactgg aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc
    18901 tggaagaggc cggaatgccg gaagataaag tgatgcaggt aagcgcaatg gcggaccaga
    18961 tgctgctgga cgcgaagaat ccgcaaagcg cgggcaatcg gcgtattgag attatggtgc
    19021 tgaccaaaag tgcgtccgat acgctgtatc aatactttgg tcagcatggg gataaagtgg
    19081 tgcagccgct ggtgcaaaag ctggataagc agcaggtgct ttcgcagcga atgcgctaaa
    19141 tgctgaatct ttacgcattt ctcaaaacct gaaatcactg tatactttac cagtgttgag
    19201 aggtgagcaa tgcgtaaaat cattcatgtg gatatggact gctttttcgc agcggtggag
    19261 atgcgcgaca atcccgctct gcgcgatatc cctattgcta ttggcggcag ccgcgaacgt
    19321 cggggggtga tcagcaccgc caattatccc gcgcgtaaat ttggcgtacg tagcgctatg
    19381 cctactggga tggcgctcaa attatgccca catctcacct tgcttccggg gcgctttgac
    19441 gcctacaaag aagcctcaaa tcatatccgt gaaatcttct cgcgctacac ctcgcgcatt
    19501 gaaccgttgt cactggatga ggcttatctc gatgtcaccg atagcgtcca ttgccacggt
    19561 tctgcgaccc tcatcgccca ggaaatccgc cagacgatct tcaacgagct gcaactgacg
    19621 gcatctgcgg gcgtggcacc agtaaagttt ctcgccaaaa tcgcctccga catgaataaa
    19681 cccaacggtc agtttgtgat tacgccggca gaagttccgg catttttaca aaccttacca
    19741 ctggaaaaaa tccccggcgt cggcaaagtc tcggcggcaa aactggaagc gatggggctg
    19801 cggacctgcg gtgatgtaca aaagtgtgat ctggtgattc tgcttaaacg ctttggcaaa
    19861 tttggccgca ttttgtggga gcgtagtcag gggattgacg agcgcgacgt taacagcgaa
    19921 cggttacgaa aatccgtcgg cgtggaacgc acgatggcgg aagatattca tcactggtct
    19981 gaatgtgaag cgattatcga gcggctgtat ccggaacttg aacgccgtct ggcaaaggta
    20041 aaacctgatt tactgattgc tcgccagggg gtgaaattaa agttcgacga ttttcagcag
    20101 accacccagg agcacgtctg gccgcggctg aataaagctg acttaatcgc caccgcgcgt
    20161 aaaacctggg atgaacgccg cggcgggcgc ggtgtgcgtc tggtggggct gcatgtgacg
    20221 ttgcttgacc cgcaaatgga aagacaactg gtgctgggat tatgatgtat actattatgt
    20281 atattctggt gtgcattatt atgagggtat cactgtatgc atcgaattct cgctgaaaaa
    20341 tcggtcaata tcactgagtt acgtaaaaac ccagctaaat actttattga tcaaccagtt
    20401 gcggttcttt ctaataatcg ccccgcagga tatctcttaa gtgccagcgc attcgaagcg
    20461 ttaatggaca tgcttgctga acaagaggag aaaaagccca taaaggcgcg ctttcgtcca
    20521 agtgctgcaa gattagagga aattacacgc cgcgctaaac aatatcttaa tgatatgacg
    20581 gatgatgatt tcaatgactt taaggaataa ggatgcgggt attcaaaaca aaacttattc
    20641 gcctgcaact tacagcagag gaacttgatg cgttaacggc ggattttatt tcctataagc
    20701 gtgacggtgt tttgccagat atatttggtc gcgatgcact ctacgacgac tcgtttacct
    20761 ggccattaat caaatttgag cgagttgctc atattcatct ggcaaatgtg aataatccat
    20821 ttccgccaca gttgcgccaa ttcagcagaa cgaatgacga agcgcatttg gtatattgtc
    20881 agggtgcgtt tgatgagcaa gcatggttgc tcattgccat tctgaagcct gaacctcata
    20941 aactggctcg agataacaac caaatgcata aaattggaaa aatggcagaa gcgtttcgca
    21001 tgcgtttttg aatttatatt atgaataaca tacaaataag agattatcag cctggcgatt
    21061 ttcagcaact atgcgctatt ttcattagag cggttacgat gaccgtcagt cagcattatt
    21121 cactacaaca aattgccgcc tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg
    21181 cgaaatcaca agtgcgggtt gcagttatta atgcaaaact ggtcggcttt attacctgcg
    21241 ttgaacacta tatcgatatg ttatttgttg accctgaata cacccgccgt ggggttgcca
    21301 gcgctttgtt aaaacctttt attaagtctg aatccgaact tacggtggac gcaagcataa
    21361 ccgcaaaacc cttttttgaa cgttatggtt ttcagacagt taagcagcag cgcgttgaat
    21421 gccggggagc gtggtttatt aatttttata tgcgttataa accgcaacat taaatccagc
    21481 ttgcaatgaa aataacgccc gcctagtatg tgcgcgtttt cttattccac aaactgcaag
    21541 gaggtaaatc atgacaaatc ctttatcaat gactctttgc agacctttcc aggattaatt
    21601 ctttttttct tgccctggat tcgtctgcca tttcctgatt ttttaattgt tactctcaat
    21661 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
    21721 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
    21781 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
    21841 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
    21901 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
    21961 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
    22021 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
    22081 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
    22141 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
    22201 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
    22261 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
    22321 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
    22381 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
    22441 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
    22501 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
    22561 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
    22621 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
    22681 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
    22741 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
    22801 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
    22861 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
    22921 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
    22981 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
    23041 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
    23101 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
    23161 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
    23221 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
    23281 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
    23341 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
    23401 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
    23461 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
    23521 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
    23581 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
    23641 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
    23701 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
    23761 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
    23821 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
    23881 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
    23941 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
    24001 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
    24061 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
    24121 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
    24181 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
    24241 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
    24301 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
    24361 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
    24421 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
    24481 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
    24541 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
    24601 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
    24661 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
    24721 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
    24781 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
    24841 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
    24901 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
    24961 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
    25021 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
    25081 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
    25141 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
    25201 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
    25261 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
    25321 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
    25381 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
    25441 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
    25501 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
    25561 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
    25621 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
    25681 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
    25741 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
    25801 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
    25861 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    25921 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
    25981 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
    26041 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
    26101 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
    26161 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
    26221 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
    26281 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
    26341 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
    26401 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
    26461 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
    26521 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
    26581 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
    26641 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
    26701 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
    26761 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
    26821 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
    26881 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
    26941 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
    27001 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
    27061 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
    27121 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
    27181 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
    27241 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
    27301 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
    27361 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
    27421 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
    27481 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
    27541 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
    27601 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
    27661 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
    27721 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
    27781 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
    27841 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
    27901 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
    27961 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
    28021 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
    28081 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
    28141 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
    28201 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
    28261 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
    28321 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
    28381 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
    28441 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
    28501 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
    28561 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
    28621 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata tt
//
LOCUS       DAJTMX010000042        28420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTMX010000042 DAJTMX010000000
VERSION     DAJTMX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.042"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            564..1334
                     /locus_tag="OO145_001836"
     CDS             564..1334
                     /locus_tag="OO145_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5466971.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO145_001837"
     CDS             1325..1798
                     /locus_tag="OO145_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5466972.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO145_001838"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO145_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5466973.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO145_001839"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO145_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5466974.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO145_001840"
     CDS             3233..3727
                     /locus_tag="OO145_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5466975.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO145_001841"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO145_001841"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5466976.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO145_001842"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO145_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO145_001843"
     CDS             7557..8471
                     /locus_tag="OO145_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5466977.1"
                     /translation="MSRSQNLRHNVINQVIDDIARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO145_001844"
     CDS             8686..10047
                     /locus_tag="OO145_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5466978.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO145_001845"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO145_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5466979.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO145_001846"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO145_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5466980.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO145_001847"
     CDS             12597..13886
                     /locus_tag="OO145_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5466981.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO145_001848"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO145_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5466982.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO145_001849"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO145_001849"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5466983.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO145_001850"
     CDS             16212..16592
                     /locus_tag="OO145_001850"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5466984.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO145_001851"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO145_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5466985.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGCDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO145_001852"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO145_001852"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5466986.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO145_001853"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO145_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5466987.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO145_001854"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO145_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5466988.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO145_001855"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO145_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO145_001856"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO145_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO145_001857"
     CDS             23321..23833
                     /locus_tag="OO145_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5466989.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO145_001858"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO145_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5466990.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO145_001859"
     CDS             26426..26629
                     /locus_tag="OO145_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5466991.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO145_001860"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO145_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5466992.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>28420)
                     /locus_tag="OO145_001861"
     CDS             complement(27665..>28420)
                     /locus_tag="OO145_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5466993.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6954 a         7426 c         7126 g         6914 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atagcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agctatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattagatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca ggcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggctgcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcctgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcttgagga agaatatgct
    27961 caattgtgat tctccccgta taggttttaa tcacgctctc atcttgttgc tccatatcta
    28021 agcgaagcaa aattgctttt attaagttca cttgattcgg acgaggctca tataaatctt
    28081 catccagagc agctataaaa cctgaattat ctgcatgttg gtttatctga tttatcacag
    28141 aatcaaaagg catatcatta tttattgcta ccagagcaga ataacaaacc atttctcttt
    28201 ggcttttaat ttgttttttt aaccacccat gcatatatac cttctcaaag gcggtaataa
    28261 attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc cattcgattc atgaatgcca
    28321 taaccggagg aatccactca tctaccccta aattacttag cgatgctatt tttcgtcgta
    28381 ttgaagggtg ttcaaaatca ttctctaaaa tcttagtata
//
LOCUS       DAJTMX010000043        28406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTMX010000043 DAJTMX010000000
VERSION     DAJTMX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.043"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO145_001862"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO145_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5466994.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="OO145_001863"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="OO145_001863"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5466995.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="OO145_001864"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="OO145_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5466996.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="OO145_001865"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="OO145_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5466997.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="OO145_001866"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="OO145_001866"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5466998.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="OO145_001867"
     CDS             6428..7057
                     /locus_tag="OO145_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5466999.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO145_001868"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO145_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5467000.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="OO145_001869"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="OO145_001869"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5467001.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="OO145_001870"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="OO145_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5467002.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="OO145_001871"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="OO145_001871"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5467003.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="OO145_001872"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="OO145_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5467004.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="OO145_001873"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="OO145_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5467005.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="OO145_001874"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="OO145_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5467006.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="OO145_001875"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="OO145_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5467007.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="OO145_001876"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="OO145_001876"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5467008.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO145_001877"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO145_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5467009.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO145_001878"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO145_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5467010.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="OO145_001879"
     CDS             complement(21748..22626)
                     /locus_tag="OO145_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5467011.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO145_001880"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO145_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5467012.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25254..25589
                     /locus_tag="OO145_001881"
     CDS             25254..25589
                     /locus_tag="OO145_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5467013.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25747..26190
                     /gene="yjdN"
                     /locus_tag="OO145_001882"
     CDS             25747..26190
                     /gene="yjdN"
                     /locus_tag="OO145_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5467014.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26323..27111
                     /gene="phnC"
                     /locus_tag="OO145_001883"
     CDS             26323..27111
                     /gene="phnC"
                     /locus_tag="OO145_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5467015.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27136..28152
                     /gene="phnD"
                     /locus_tag="OO145_001884"
     CDS             27136..28152
                     /gene="phnD"
                     /locus_tag="OO145_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5467016.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28258..>28406
                     /locus_tag="OO145_001885"
     CDS             28258..>28406
                     /locus_tag="OO145_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5467017.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7297 a         7419 c         6935 g         6755 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgtaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccacgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gttgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gagctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg acctatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt ttatcagaat aaataacgtc ttctgatgcg tttaaaacgt cagaaagata
    25021 aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa
    25081 atagaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc
    25141 taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg
    25201 gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat
    25261 taccacactg cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct
    25321 gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga
    25381 tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag
    25441 atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc
    25501 gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac
    25561 tgaaatctga gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg
    25621 ctcgcgccgc atccggcaac gatgccgact gcctgatgcg acgcttgcgc gtcttatcag
    25681 gcagcctctc atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc
    25741 accatcatgc cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc
    25801 tattatcaac gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg
    25861 aaatcagcgc aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc
    25921 atcgctcatg ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgctataccg
    25981 tcaggaaaag ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa
    26041 ggaaaacgct ggtttgataa ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa
    26101 actttctggg cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt
    26161 aatgtcgtca aacaacaacc aacgcaataa cccgccggga ggcccgccct cccgcactgt
    26221 catcgaattc ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa
    26281 taatccgcca cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt
    26341 gtcgagaagc tcgccaaaac cttcaatcag catcaagcgc tgcatgcggt tgatctgaac
    26401 attcatcaca gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt
    26461 ttacgtcatt taagcggttt gattaccggc gataaatccg ccggtagcca tatcgagcta
    26521 ctgggccgca ctgtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc
    26581 aacaccggct acatctttca acaattcaac ctggtgaacc gcctgagcgt actggagaac
    26641 gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca cctgttttag ctggttcacc
    26701 ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcattttgcc
    26761 catcagcgcg tttccaccct ctccggcggc cagcagcaac gtgtggcgat tgcccgtgcg
    26821 ctgatgcagc aggcgaaagt gattctggcc gatgaaccca tcgcctcgct ggacccggaa
    26881 tcagcgcgca tcgtgatgga caccctgcgc gacatcaacc agaacgacgg catcaccgtg
    26941 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
    27001 cgtcaggggc acgtcttcta cgacggcagc agccaactgt ttgataacga acgttttgac
    27061 catctctacc gcagcattaa ccgcatcgaa gagaacgcga aagctgcctg atatccccat
    27121 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
    27181 agcctcagca ccctgttaac cccggcacac gccgaagagc aggaaaaggc gctgaatttc
    27241 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gtttttacag
    27301 gatatggaga agaaactggg cgtgaaggtc aacgccttct ttgccccgga ctacgcgggc
    27361 attatccagg ggatgcgctt caataaagtg gatattgcct ggtacggcaa cctgtcggcg
    27421 atggaagcgg tggatcgcgc caatggccag gtcttcgccc agacggtcgc ggcggatgga
    27481 tcgccgggtt actggagcgt gttgatcgtc aacaaagaca gcccgatcaa caacctgaac
    27541 gatctgctgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct
    27601 ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttc
    27661 aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaagtg
    27721 gatgttgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag
    27781 cttaaagagc tgaaggtgat ctggaaatcg ccgctgatcc taggcgatcc gatcgtctgg
    27841 cgcaagaatc tgtccgaaac taccaaagac aagatctacg acttctttat gaactacgga
    27901 aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct gggcgccgtt ccgcgcttcc
    27961 agcgacctgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat acaaagcgtg
    28021 aaaggcaata aagggctgaa tgagcaggac aagctggcga aaaccaccga gattcaggcg
    28081 cagctggatg acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa
    28141 gcggtgcagt aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgtag
    28201 gcgttgatgc cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg
    28261 caaaccatca ccatcgcccc acccaagcgt agctggttct cgcttctgag ctgggccgtt
    28321 gttctcgacg tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt
    28381 aaagacggcg gcaacatggc aacctt
//
LOCUS       DAJTMX010000044        27601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTMX010000044 DAJTMX010000000
VERSION     DAJTMX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.044"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO145_001886"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO145_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5467018.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO145_001887"
     CDS             complement(1614..1937)
                     /locus_tag="OO145_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5467019.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO145_001888"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO145_001888"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5467020.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO145_001889"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO145_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5467021.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO145_001890"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO145_001890"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5467022.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO145_001891"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO145_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5467023.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO145_001892"
     CDS             7537..8574
                     /locus_tag="OO145_001892"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5467024.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO145_001893"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO145_001893"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5467025.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO145_001894"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO145_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5467026.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO145_001895"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO145_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5467027.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO145_001896"
     CDS             10745..11626
                     /locus_tag="OO145_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5467028.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO145_001897"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO145_001897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5467029.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO145_001898"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO145_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5467030.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO145_001899"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO145_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO145_001900"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO145_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5467031.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO145_001901"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO145_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5467032.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO145_001902"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO145_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5467033.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO145_001903"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO145_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5467034.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO145_001904"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO145_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5467035.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO145_001905"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO145_001905"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5467036.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO145_001906"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO145_001906"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5467037.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO145_001907"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO145_001907"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5467038.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO145_001908"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO145_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5467039.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO145_001909"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO145_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5467040.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO145_001910"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO145_001910"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5467041.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO145_001911"
     CDS             24893..25273
                     /locus_tag="OO145_001911"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5467042.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO145_001912"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO145_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5467043.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO145_001913"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO145_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5467044.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="OO145_001914"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="OO145_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6865 c         7236 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAJTMX010000045        27573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTMX010000045 DAJTMX010000000
VERSION     DAJTMX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.045"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(24..278)
                     /locus_tag="OO145_001915"
     CDS             complement(24..278)
                     /locus_tag="OO145_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5467045.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO145_001916"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO145_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5467046.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO145_001917"
     CDS             1228..2694
                     /locus_tag="OO145_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5467047.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO145_001918"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO145_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5467048.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO145_001919"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO145_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5467049.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO145_001920"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO145_001920"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5467050.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO145_001921"
     CDS             5709..7265
                     /locus_tag="OO145_001921"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5467051.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO145_001922"
     CDS             7347..9161
                     /locus_tag="OO145_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5467052.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO145_001923"
     CDS             9162..10256
                     /locus_tag="OO145_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5467053.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO145_001924"
     CDS             10256..11281
                     /locus_tag="OO145_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5467054.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO145_001925"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO145_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5467055.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO145_001926"
     CDS             complement(12876..13220)
                     /locus_tag="OO145_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467056.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO145_001927"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO145_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5467057.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO145_001928"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO145_001928"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5467058.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO145_001929"
     CDS             15616..17376
                     /locus_tag="OO145_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5467059.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO145_001930"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO145_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5467060.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO145_001931"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO145_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5467061.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO145_001932"
     CDS             19113..19340
                     /locus_tag="OO145_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5467062.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO145_001933"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO145_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5467063.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO145_001934"
     tRNA            21195..21271
                     /locus_tag="OO145_001934"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO145_001935"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO145_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5467064.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO145_001936"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO145_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5467065.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO145_001937"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO145_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5467066.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO145_001938"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO145_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5467067.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..>27573)
                     /gene="ccmF"
                     /locus_tag="OO145_001939"
     CDS             complement(26746..>27573)
                     /gene="ccmF"
                     /locus_tag="OO145_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5467068.1"
                     /translation="PLVHKQLGLGSISIGEPFFNTMFTWLMVPFALLLGVGPLVRWGR
                     DRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVAMTVLGLAMACWIAVLATAEAALRI
                     SRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYSVERDVRMKSGDSVDIHEYRFTFRD
                     VKEVTGPNWRGGVATIGVTREGKPETVLYAEKRYYNTAGSMMTEAAIDGGITRDLYAA
                     LGEELENGAWAVRLYYKPFVRWIWAGGLMMALGGLLCLFDPRYRKRVSPQKTAPEAV"
BASE COUNT         6767 a         7168 c         6731 g         6907 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cgg
//
LOCUS       DAJTMX010000046        26789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTMX010000046 DAJTMX010000000
VERSION     DAJTMX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.046"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1173
                     /gene="menE"
                     /locus_tag="OO145_001940"
     CDS             <1..1173
                     /gene="menE"
                     /locus_tag="OO145_001940"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5467069.1"
                     /translation="TPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALV
                     PDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASA
                     QGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHA
                     SLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVC
                     AKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDR
                     GEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGHRPV
                     AVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ"
     gene            1278..1550
                     /gene="pmrD"
                     /locus_tag="OO145_001941"
     CDS             1278..1550
                     /gene="pmrD"
                     /locus_tag="OO145_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5467070.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(1544..1930)
                     /gene="arnF"
                     /locus_tag="OO145_001942"
     CDS             complement(1544..1930)
                     /gene="arnF"
                     /locus_tag="OO145_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5467071.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(1930..2265)
                     /gene="arnE"
                     /locus_tag="OO145_001943"
     CDS             complement(1930..2265)
                     /gene="arnE"
                     /locus_tag="OO145_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5467072.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(2262..3914)
                     /gene="arnT"
                     /locus_tag="OO145_001944"
     CDS             complement(2262..3914)
                     /gene="arnT"
                     /locus_tag="OO145_001944"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5467073.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            complement(3914..4804)
                     /locus_tag="OO145_001945"
     CDS             complement(3914..4804)
                     /locus_tag="OO145_001945"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5467074.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(4801..6782)
                     /gene="arnA"
                     /locus_tag="OO145_001946"
                     /pseudo
     CDS             complement(4801..6782)
                     /gene="arnA"
                     /locus_tag="OO145_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            complement(6782..7750)
                     /gene="arnC"
                     /locus_tag="OO145_001947"
     CDS             complement(6782..7750)
                     /gene="arnC"
                     /locus_tag="OO145_001947"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5467075.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(7754..8893)
                     /gene="arnB"
                     /locus_tag="OO145_001948"
     CDS             complement(7754..8893)
                     /gene="arnB"
                     /locus_tag="OO145_001948"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5467076.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDVYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            9201..9803
                     /locus_tag="OO145_001949"
     CDS             9201..9803
                     /locus_tag="OO145_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5467077.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(9842..10267)
                     /gene="nudI"
                     /locus_tag="OO145_001950"
     CDS             complement(9842..10267)
                     /gene="nudI"
                     /locus_tag="OO145_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5467078.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            10546..11088
                     /locus_tag="OO145_001951"
     CDS             10546..11088
                     /locus_tag="OO145_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467079.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            11175..12377
                     /locus_tag="OO145_001952"
     CDS             11175..12377
                     /locus_tag="OO145_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5467080.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            12432..12902
                     /locus_tag="OO145_001953"
     CDS             12432..12902
                     /locus_tag="OO145_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5467081.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     MALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(12943..13893)
                     /locus_tag="OO145_001954"
     CDS             complement(12943..13893)
                     /locus_tag="OO145_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5467082.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(14086..15276)
                     /gene="glpC"
                     /locus_tag="OO145_001955"
     CDS             complement(14086..15276)
                     /gene="glpC"
                     /locus_tag="OO145_001955"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5467083.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(15273..16532)
                     /gene="glpB"
                     /locus_tag="OO145_001956"
     CDS             complement(15273..16532)
                     /gene="glpB"
                     /locus_tag="OO145_001956"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5467084.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(16522..18150)
                     /gene="glpA"
                     /locus_tag="OO145_001957"
     CDS             complement(16522..18150)
                     /gene="glpA"
                     /locus_tag="OO145_001957"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5467085.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            18423..19781
                     /gene="glpT"
                     /locus_tag="OO145_001958"
     CDS             18423..19781
                     /gene="glpT"
                     /locus_tag="OO145_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5467086.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            19786..20862
                     /gene="glpQ"
                     /locus_tag="OO145_001959"
     CDS             19786..20862
                     /gene="glpQ"
                     /locus_tag="OO145_001959"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5467087.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(20904..21146)
                     /locus_tag="OO145_001960"
                     /pseudo
     CDS             complement(20904..21146)
                     /locus_tag="OO145_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            21325..21975
                     /gene="inaA"
                     /locus_tag="OO145_001961"
     CDS             21325..21975
                     /gene="inaA"
                     /locus_tag="OO145_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5467088.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(22029..22283)
                     /gene="yfaE"
                     /locus_tag="OO145_001962"
     CDS             complement(22029..22283)
                     /gene="yfaE"
                     /locus_tag="OO145_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5467089.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(22283..23413)
                     /gene="nrdB"
                     /locus_tag="OO145_001963"
     CDS             complement(22283..23413)
                     /gene="nrdB"
                     /locus_tag="OO145_001963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5467090.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(23647..25932)
                     /gene="nrdA"
                     /locus_tag="OO145_001964"
     CDS             complement(23647..25932)
                     /gene="nrdA"
                     /locus_tag="OO145_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5467091.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         6504 a         6613 c         7015 g         6657 t
ORIGIN      
        1 acgccgcaaa cgctgctcgc ctggctggcg ttactgcaat gcggggcgcg ggtgttgccc
       61 gtgaaccctc agctgccgca accgttgctt gaagaattgc tgcccaatct gacgctgcaa
      121 tttgcgctgg tgccggatgg ggaaaacacg tttccggcat taacgtcgct gcacattcag
      181 ctggttgaag gcgtacatgc cgctgcgtgg cagccgacgc gtctgtgctc aatgacgttg
      241 acctcgggtt ctaccggttt gccgaaagcc gctgtccata cttatcaggc ccatcttgcc
      301 agtgcgcaag gtgtgttatc gctgattccg tttggcgatc acgatgactg gttgctctct
      361 ttaccgctgt ttcacgtctc cggtcaggga attatgtggc gctggttata cgctggtgcg
      421 cggatgacgg tgcgtgataa acagccattg gagcaaatgc tggcaggctg tactcacgct
      481 tcactggtgc caacacaact ctggcgtttg ctggttaacc gcagttccgt ttccctgaaa
      541 gcggtgttac ttggcggcgc ggctatcccg gtcgagttga cggaacaggc gcgcgagcag
      601 gggattcgtt gcttttgcgg ctatggtctg accgagtttg cctccacggt atgtgcgaaa
      661 gaagccgacg gcctggcaga cgttggtttg ccgctgccgg gtcgggaagt gaaaatcgtt
      721 aataatgaag tgtggctgcg ggctgccagt atggcagaag gttactggcg taacgggcaa
      781 ctggtttcac tggttaatga cgaaggctgg tacgctacgc gcgatcgcgg tgagatgcat
      841 aatggcaagc tgaccattgt cggacgttta gacaatctgt tcttcagtgg cggtgagggt
      901 attcagccgg aagaagtcga gcgtgttatt gctgcacatc ctgcggtttt gcaggtgttt
      961 atcgtttccg ttgccgacaa ggagtttggt catcggccgg tagcggtggt ggagtatgac
     1021 cagcaaaccg ttgatcttgg cgaatgggtg aaagataagc tggctcgttt tcaacaaccg
     1081 gtgcgctggc tgactttacc gccggagctg aaaaacggcg gtattaaaat ttcacgtcag
     1141 gcgctaaaag agtgggtgca acgtcagcaa taattaaccc tccgtttcac ttctcatttc
     1201 tgccattcca ttgccgttga taaagagttt atctgttaat ctgtaaattg atgtgaaaac
     1261 tcttagcaaa caggataatg caatggaatg gctggcacaa aaaatcgtgc tgcaataaac
     1321 aagataacag acatgtactt atgctctgcg atgctggcgg cgcgataaaa atgatcgccg
     1381 aagtgaagag tgacttcgcc gtgaaagtgg gggatttact ctcgcctttg cagaatgcgc
     1441 tttattgtat taatcgtgaa aagctgcata cggtgaaagt cctctcggca agcagttata
     1501 gccccgatga gtgggaacgg cagtgtacag cggcagggaa aactcagtaa cgttgttttg
     1561 tcgtgggcag gaaaatcagc atcaacccgc tcataataca ggctactccc agtagtgctt
     1621 tcagcgaaaa agtcccttcc cagccgggaa gtaccattga agcaatccat accagcacat
     1681 aactcatact gagcaaggca taggctttgc ttagcgcaag tttatgtagc gttttatacc
     1741 aacaaaaaac ggaaagcaga taacccagca gcccgagtaa cagtatccgg gcatccaggc
     1801 caaaagctaa cagagccgcg ataaaatccc ataagtgcgt catcggcggc agatgacttg
     1861 ccgcaaaacc caggcttaat tgcgcaactg aggcaataat gacgctgaat aatccccata
     1921 tcaggcacat tacaccgtac tcccgaggat cacaatgcca ccaataatga acgccacccc
     1981 acaccagtga cgcggcgata ccggttcgtg ccacagtttt actgcagcca gcgtcaccca
     2041 gacaaaattc agacttaaca tcgggtaggc aatgcctacc ggtacgttct gcaagaccag
     2101 cagccagagc atcatggcaa gaccaagaca agccagcgcc agtcccagcc acagcacgat
     2161 atgtttgcgc cgtttgttta tcgccacaaa gcaggttgcc tgtttctgac acaactgccc
     2221 ggcaacgcta agcaagctgg caaagactaa tgttagccag atcatttggg acgatactga
     2281 atcagcacca gacgctcctg acgaacgatg gcatctgcgg gcggaatggc gagactgttg
     2341 atatcttcat cgcggtcaac cgaaagcacg agtgtaataa tcccctcctg acgatgttga
     2401 ttaagccagt ttgcgaactc atcaccgctg acaaatctcc ctttcgcatc cggataatta
     2461 aggccgtatt tcaactcacc tgtctggcga tacatgatga tgtcatcgcg ttgcaggctc
     2521 catgccagac ctgcggcaac accgacgcta tcagtaagaa tatagcggct tggctgaagt
     2581 gattcttgtg tcatctcgac aaaaaattgc ggatgttttc cttccatgac tctgtcagga
     2641 attgaaaatc ctaccagcaa cgccagcccc agcgggcaaa gcgcggcgaa agaccaggtc
     2701 ttttcgacgt ttgttaaggt gtaccagccg aaaaatgccc atagcgaaaa aatcgaccag
     2761 gcacaaaaga ctttatagct ctcgaaggtt tgccacaccg gcgtgttcat tggtccccag
     2821 ggggagatca caaatgtggc aataatgcca gtgacgccaa aagcgatgtt aatccagcca
     2881 ttaatccgca gcgccagagg attatttttt gctgccagca acgcgtaatg cgccaacagc
     2941 attgccagag gtgcaaagca agaaagaata taggtgggca atttgccttt agcgacggag
     3001 aaaaacagca gcggcattat cgtccagctc aacaaataga cggttgcgga atgcttgcgg
     3061 tttttccagc ctgtgtacag tgcaccgggg agtaatccca gccacggcag gctaccggca
     3121 atgatgaccg gcacgtagta ccagaacgga gctctatgtt gggcatcatc tagtgcaaag
     3181 cgttgaatat gctcaaccca gaaaaaatag tgccagaagt caggctcccg ctgcgctatc
     3241 gccagtcccc aggggagaac cgtcagtacg caactgataa ccgccagcca gccgtaaata
     3301 aagagatctt tccagcgttt ttgcgtcgct acccatggca gcacgcttaa taccggcacg
     3361 gcaagggcga gaaaaccttt ggtcatcacc cccatgccgc aggtgattcc cagcagtaaa
     3421 aatcctgcgc ttttgccttt ccacgtctgt gcctgcattg ccagccagaa gctgcacatt
     3481 cccgccacca gccagaatgc aataaacgga tcgagcacgg cataagtgcc gatggcatag
     3541 acaataaaca atgagagata aattactgtg gcgagtagag ccagacgttt atcgcgccat
     3601 aagcgcagag taaaccaggt caccagcgcg gcagttaaca gggtcgcaaa gataacgcct
     3661 gcccgcacac caaagttatt cgcgccaaat agccattgcc caatgctgtt aatccagtat
     3721 ccggcaatgg gtttttcgaa ataacgtagc cctaacagat ggggcacaat ccagtcgccg
     3781 gatgccagca tttctcgact gatttccgca taacgcgttt catcgggttg ccagagcaga
     3841 cgcgtgctga tcggtaacag gtaatagcag gcaataaacg cgaagatgcc gataaggtaa
     3901 cgtaccgatt tcatcagcga ctacccgcaa tttgttggca acccagccag ccttcacgtc
     3961 cggcaatatt tccgcgaaca acttgtccga gcggcaacgt ctctgataac agttcgctta
     4021 aagggcaaaa tgtcacgcct tcctgagctg cgcgtttgag gagatccaca aaattatgct
     4081 gataagcgca gccttcaact tctgcatgaa tggtataaac cggcgtgcct ttatctcgca
     4141 ggatgcggtt gagtaaccaa ccgttaaaat cttctgcttt cacatcccga ccaatcactt
     4201 catcccaggt gggtaaggtc accggaattt gcgcagtgcc agggtttcct gattcaagca
     4261 gtggacggaa cggtatggcc ccacgacaat cgctgttgta gcgcaaatgg aacgcttctt
     4321 ttgcttcgat caccttctgg tcggcacgcc agcccgcagc ggcagaacag gttaccggtt
     4381 gaccgataat ctcttccaga gtgcgaagac cgcgtgcaat atcgtcgatc attgtttgcc
     4441 gatcccagtt accgctacgg gcttgccagg catggtgatc ccaggcgtgc agaccgactt
     4501 cgtgatgttt agccgcttca cgaatgatat cggcattggc atgaccaatc tctttacctg
     4561 gccaggccgt acctgccagt aaaatatccc agccataaag cgatgccgcg tttgagcgca
     4621 gcatcttcca caaaaactgt ggcttcacca gtcgccagag atggcggccc atattgtccg
     4681 ggccgacgct gaaaaaaatg ctggcctgaa tattatgctt actcaagatt tccagcagac
     4741 gcggcacgcc ttcacgggtg ccacgaaagg catcgacatc aatgcgtaag cctactttgg
     4801 tcatgatggt ttatccgtaa gatcaacggt gcgcaggaag aaatccagcg tttcgtcgat
     4861 ggtttcctgc atatcaattt tcggctccca gtccaggcag cggtgggcat tgcggatgct
     4921 cggtttacga tgctctacgt cctgatatcc tttgccgtag tagcagctac tttcgacaac
     4981 gcgaaagccc gcaaacggtg ggaaatgatg gcgcagcgga tgtttttcga agctcgccag
     5041 cagcatctcg cccagttcct caatgctcgc ttcgttctca ggattgccaa tgttgataat
     5101 ttcgccgtcg cagcgatttc ccgcattttc gataatgtgg tacaacgcct cgataccgtc
     5161 ccggatatca gtaaagcagc gtttttgttt tccgccatca attagcttaa ttggtgaacc
     5221 ttctaccaga ttgagaatga gttgcgtaat agcgcgggag ctgccaattc gcgctgcatt
     5281 aaggttatcc agtcgtggtc ccatccagtt aaacgggagg aagagggtga actgtaaacc
     5341 ctctttttcg ccataggccc agatcacccg atcaagtaat tgttttgaca ccgaataaat
     5401 ccagcgtggt ttattcaccg ggccgacgat taaattagaa tggtcctcat cgaagtattt
     5461 atcgctacac atcccataaa cttctgaagt tgacgggaag atgattcgct tacggtactt
     5521 aacgcagtag cggataatgc gcagattctc ttcaaaatcg agttcaaata cgcgcagcgg
     5581 gttgcgggta tattcaatcg gcgtggctat cgccaccagc ggcaagacga catcacattt
     5641 tttgacgtga tactcaatcc attcggaatg aatactgata tcgccttcga caaagtgaaa
     5701 atgtggatga ttcagaaaac ggcttatcgc atcgctgcca atatccagac cgtaaacttc
     5761 ataatgatct tcgcgcagca ggcgttctgt cagatggttg ccaataaagc cattcacccc
     5821 gaggatgagt acccgggtac ggcgtcgggc ggtgcaggca ggctggctat tcaagcgtga
     5881 accttgcacc aggcccagca tctgcgctaa ttgcgagccc tgcatagtaa tgccgtcgcc
     5941 cgcctgtccg gtgacgattt ccagcgcgcc atcgccacag gcaatcagca gtggcgcaac
     6001 agaaatcacg ctccccggct gtgctttgct ggcatgagga tgaacacgcg acgaccagac
     6061 ggtgaatttc tgattgccaa catagctgaa ggcacccggc cacggatcgg caacggcacg
     6121 taccatgttg tgcagtacgg atgccggttt atgccattca aggaaactgt catccggcgt
     6181 tctgcgacca aaacaggtgg cttcgttttc gcgctgggcg atttccagaa tattaccgtg
     6241 tttaatggcg ggtaatgtct gttccagtag ctggcgcgcg gcatggcaca atttatgatg
     6301 cagcgtaata gcgatatcgt ctggcgcaat ggcaacgcgc agttgcgcca caatggcccc
     6361 agcatcggca cgtttaccat tcggtgcaat gtaacgcccg tttccgtttc accgttcacc
     6421 agcacccagt tcagcggcgc gcgaccacga tattttggta acagcgaacc gtgcagatta
     6481 aatgcacctg cgggagcgag ctgaaaaatc tcgtcgcaaa taagatggcg ataataaaaa
     6541 gagaaaatca cctctggtga cagttgggca atgcgttcca cccacagcgg atgattaacg
     6601 ttatccggcg cataaaccgg aatgtctctt tccgccgcca gatgagccac cgaaccataa
     6661 aaggcttttt caccgggatt atcggtatgg gtaaaaatgg cgctaatttc gtaaccggca
     6721 gccagcagag cttcaatacc gaggcatccc atatcgtggt aggcaaaaac gacggttttc
     6781 attattcatt ttccttgctg gatggacgga taacttgctg aacaaaatag cgggggcggg
     6841 cgcggacatc ggtgtagatc ctgccgatat attcaccgag taatcccatg ccgataaact
     6901 gagcgccaat aaaagtaaac agcacggcaa atagcataaa gacgccttct gccgcccatt
     6961 gtggtccgaa ggtcaggcgt aaaatcacca gcagcaccgc aatgctaaaa cctccaatcg
     7021 caataatgct gccgagcaga ctcagcatac gtagcggcgt tgtggtaagg caggtcacca
     7081 ggtcgtacat caaattaatc aggcgcataa aactgtattt ggattcacca aactcacgct
     7141 cggcatgatg tactggaatt tcaattgcac ggcgggcgaa gatattcgcc agaatcggga
     7201 taaaggtgct gcgttcatgg cagtgcaaca tcgcatcgac aatatgacgg cgataggcgc
     7261 gcagcataca accgtagtca cccatcgctt tgccagtggt gcgctgaata agccggttaa
     7321 tcatcttcga agctgtttta cgaaaccagc tgtcctggcg gttctggcgt acagtcccta
     7381 ccacgtcgta accttcatcg gcttttgcca ccaggcgggg gatttcttct ggcggattct
     7441 ggagatcggc atcaagggta ataattaagt cgccagtaac gtgactgaat cccgccataa
     7501 tcgctgaatg ttgcccgtaa ttgcggttaa tcaaaataga cacaatatgg ctgttctccg
     7561 cttgtgaggc ttcgaccagc atatgcgcgg aattatcgct actgccgtca tcaatcagca
     7621 ggatctcata ctctttcccc aacgattcac aggctgtggt ggtgcgcctg attaattccg
     7681 gtaagctttc ctgctcgtta taaacgggaa taaccaccga gactttctta acagggtgga
     7741 tttcaaacat ggcttattgt cctgcgagtt gctgaagggc tgtgatgacg cggtcggcat
     7801 cggcggtagt catatccggg aacagcggca acgaacagat gcgttcgcta ttccattcgg
     7861 tattcggtaa cgacagcgtg ggaaaacgct cgcgataata tttttgtgtg tgagcggcgc
     7921 ggaaatgtaa cccggtacca atgcctcttt cttttaacgc ttccatcaac gcatcgcgac
     7981 tgataccaca acgttgttca tcgacacgaa taataaacag atgccaggcg tgaacgtgcg
     8041 gccaggcggg aaggcttaat ggctgaaagg ggagagctgc cagtgcttgc tgatattgct
     8101 gggcaatttc gcgccgacgg gtgttgaggt gctctaattt gactaactgt gtcagggcaa
     8161 tcgcggcgtt aatatcggtc agattgtact tatagcccgg tgttaagact tcagcctgcg
     8221 gtgcacggcc ccaggtttgt ctgtcataga catcgacacc cagaccgtga aatttcagca
     8281 tccgtagctg gcgggcaagg ttttcattat cagttacaat caggccacct tcagcacagg
     8341 taatattttt aatggcatga aatgaaaaaa tagcggtacc ttttgcgcca atatgtcgcc
     8401 ctttgtaata cgtaccgacg gcatgggcag catcttcgat aactgcgatg ccgtaacgtt
     8461 cgccaatggc gcgaatggcg tcaatatctg ctggcgcacc ggcataatgc accggaatga
     8521 tggctttagt gcgtggcgta atggctgatt cgatcgcttc aggcgtgacc atcagcgtat
     8581 cgcggtcgac atccaccatt accggcgttg cacccagcaa ggaaatcata ttgagggttg
     8641 aaacccaggt cagggaaggc gtaatcactt catcgccctt gccaatttcc aacgccatta
     8701 gcgtgatatg cattccggcg gtggctgaac tgaccgcgat ggcatgctga tttcccgtca
     8761 actggcaaaa ggcttgctca agcgcctgat tcttcggacc ggttgtgatc caaccggatt
     8821 cgagaacctc tttaactgca gcgagttcct ccacgcccat tgctggtcgc gaaaaaggca
     8881 aaaattctga cattgctttt ccttccgcca ttgaatacct gtccacttat atttgctata
     8941 aagagtgttg tgtatatttt gccatttgga gcgaaatttt aaggatagaa tattaactta
     9001 accttaagaa actaatatta gacgtaaata ttgaaatttt tatatttttt cttatttagg
     9061 ctttgcattt ggcaaaaatt tgaggcattt tgccgacatc gtaggatttt taatattaca
     9121 ccaactgcta attatcgcca gaaatgtcgc tcaatttcac ggtaattgtc tggttgcgct
     9181 tgtctatagg tggagtttac gtgttagctt tttgccgctc atcattgaag tcaaaaaaat
     9241 atttcatcat tttactggcg ctcgctgcaa ttgccggact gggtactcat gccgcctgga
     9301 gtagcaatgg tttgccacgt atcgacaata aaacattggc cagactggca cagcagcacc
     9361 cggttgtcgt tttgtttcgc catgctgaac gttgcgatcg ttcaaccaat caatgcttgt
     9421 cagataaaac aggtattacg gttaaaggta cccaggatgc ccgtgaactg ggcaacgctt
     9481 ttagcgctga catctctgat ttcgatcttt attccagtaa taccgtccgg accattcagt
     9541 cggcgacctg gttttccgcg ggtaaaaagc tgacggtaga taaacgactt cttcagtgcg
     9601 gtaatgagat ttatagtgca attaaggact tacaaagcaa agcgcctgat aaaaatatcg
     9661 ttattttcac ccataatcat tgcctgacat atattgctaa aaataagcgt gatgcgatat
     9721 ttaaacctga ttatctggat ggtttagtca tgcatgtgga aaaaggcaaa gtttatctgg
     9781 atggggaatt cgttaaccac taaattttca atctgacagc cagtgatggc tgtcatcatt
     9841 gttaaagaag acctttcaaa cgtaacgttt ttcgggtggc aacatttaaa tcataatgca
     9901 ccagatcttc aggttttacc cacgcgtagt cctgaaactc ttcgtttatt ttcacttctc
     9961 ggttggcaga aacgcagtca aaaatcaggt aaatcatata aatctcttcc ttgcgaccat
    10021 ctgcatatgt cttggtgcga atatcatcgc tgaaggtcca cggcgtgatt tctgtcaaaa
    10081 gcagctgttc tcccagttct tcgcgaattt cgcggcgtag tgcctcttca attcgttcgc
    10141 cagactccac gccgccaccc gaaagcgccc attgaccggg gaaaacgccg cgatcgtcgg
    10201 ccattttgca cagcaaataa gcaccatcat tttgaatcaa agggcataca atagtccgtt
    10261 gtcgcacagg ggtattcctt ataaataaaa agaaaccatg agaatacggt aactcagaaa
    10321 tatgtgaaag tgacagtaat ctcttttgca atgataactg cacttattat tatttttgtg
    10381 tgtatcggta aagtgtgttt attttttcgg cgtttaatta tttaagaagt atttagttac
    10441 aataagtgac tagatccctt ttttcgctca ttgaatcctc tgtgccaggc agtacaatcc
    10501 cgcagctttg ggaattttga ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg
    10561 ctagcaggtc tggccggaat gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc
    10621 ggccaggcgg gtaaagaata caccaatatt ggtgtcggtt ttgggactga atcgacgggc
    10681 ctggctttaa gcggtaactg gacacataac gacgacgacg gtgacgtcgc gggcgtgggg
    10741 ctggggttga atctgcctct cgggccgtta atggcgaccg ttggcggaaa aggcgtgtac
    10801 accaacccga attacggcga tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg
    10861 aaaattggca acagcttccg tttgtttggc gagtattact actctccgga ttcgctctcc
    10921 agcggtattc aaagttatga ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca
    10981 gtcagtattg aggcgggtta tcgctacctg aatctgtcgg gtaaagacgg taaccgcgac
    11041 aacgctgtgg ctgacggcct gtatgttggg gttaacgcca gtttctgatc ccttccggcg
    11101 cggcattcag tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa
    11161 agcaggagat aaccatgtta aaagtggaaa tgctatccac cggggatgaa gtgttatacg
    11221 ggcaaatcgt tgacactaac gctgcctggc tggccgattt tttctttcat caggggctgc
    11281 cattatctcg ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg
    11341 aacgtagtca gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg
    11401 atttaagcgc actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct
    11461 ggctcaaaga gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc
    11521 gtaaacaagc ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct
    11581 gtggttttgc cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag
    11641 aatttaaggt gatggtcgag cacgaaatcc tgccgcgcct gcgcgagcgt ttttctttac
    11701 cacagccgcc ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac
    11761 aaagcctgga cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc
    11821 cgatcatcga actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt
    11881 ggctggacgt taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc
    11941 ccgcgcagat cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat
    12001 tcaccggtgg tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg
    12061 aagtgattcc ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc
    12121 gggccaacca ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca
    12181 actttgcgct agccacgcca gacggcactt tcgctctgcg tgtgcgtttc agcactacac
    12241 gctacagcct ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctacgcc
    12301 gttggttaaa cggccaggac atcgctagtg agcatggctg gattgaggtt gttgagtcca
    12361 tgactttatc cgtctgattt cgccactctg tgccggttta ctgcaccggc acattccccc
    12421 tccggcaaaa tttgtccaac cctccctacg gtgagcgtgg tgttggggcc agtgtggcac
    12481 gggctgcgcg ctggggacgc attgagaatt acatggcgca agtcaacgat tcgctttgtc
    12541 tgttagtgca ggtggaaagt aaaatggcac tggataacct ggacgagatc ctcgacgtcg
    12601 aagggattga tggcgtgttt attggaccag cggatctttc tgcgtcgttg ggctacccgg
    12661 ataacgccgg gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg
    12721 ctgcgggtaa agcggctggt tttctggctg tggctcctga tatggcgcag caatgcctgg
    12781 cgtggggagc gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg
    12841 atcaacgact ggcgatgttt aaatcaggca aaaatgggcc acgcataaaa ggtagttatt
    12901 gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc
    12961 cagatcggct tcgcttagct gggtggccgc gagcacttgg tcacgatcaa tgccctgttc
    13021 cagcatgcgc aaagcaattt taatggcttg ttcatgcata ccttcctgca aaccttctaa
    13081 tttaccttgc tgcaaaccta ctaatttacc ttcttgccag ccaatttgat gtccttcctg
    13141 ccgtagccgt tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat
    13201 ctcctgaata aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag
    13261 tgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac
    13321 catgccgatt aaatcgtggt cgcgaatatg cttttgaatc agttccagca gagctatacg
    13381 ccgatgttgc atgatctcat cgtcaggtac gatggtgata tccaccaacg gaaaagcttc
    13441 ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca tccagccagt taagtgagta
    13501 cgggtagggc gatgtttcgc catgataaaa cagcaatggc accaccagcg gaactctgtc
    13561 atagccttta tctaggtgac tctgcatggc ggcagtggca tagcgcattg gccgaaaagc
    13621 catattcttt tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc
    13681 gctggtttcc acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa
    13741 actagtgggt tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg gttctggtaa
    13801 atgtatttcg agaaaatccc gtgcggtttc gggtgtgaat ataaaggttt taaataccgc
    13861 atcatgcggg gaggaggttg ttgattcggt catggttgtc cgtcacctga tatttatgat
    13921 gacgcgacat tacggttatc ccaggccttt gacatctgcc agtttcagcc tttgcgagac
    13981 gttttcagag gaattttcac tgcgttgcaa cgcatacatt gggcacggaa tcgtgtacgc
    14041 aaaaatgccc ggtaaccaca tgcttaccgg gcatcagaag ggagtttaag ccagcgcctg
    14101 ggccagtagc gtaatcggat gttcgcagcg aagacttgtg gacatctcaa tctgccattt
    14161 acaggtttcg cagtcggtga ccaccagatc tgcgccgctt tcttctatct ggcggaacag
    14221 tggtgcgccg atggcttgtg aggtggggta gttctctttt ttgaaaccgt aagtacccgc
    14281 aataccgcag cactgggaat ccagcaccgt taactcaagc cccgggatgt tacgcaacag
    14341 ctccagggtg tagagcgtcc agcccatttt ttccatatgg cacggagtgt gataaaccac
    14401 tttcagcggc agcggtttca gcggtaacgt tttgccttcg tccagcttgc gccacagcca
    14461 gcgggttgcc agttcgatat gatcgcgcaa gcctttgttg tcgacattca gcacttccgg
    14521 gtattcgtcg cgcagggcaa atgtacaggt tgaggaggtg gcaatcaccg gaatgccttt
    14581 tactcccaca gcttcgcgga tcgactctac attcgtaatt gcctgtttgc gtgctttatc
    14641 ggtaaagccg ttggcgatta gcggtacgcc gcagcatttt tctttgctga gcagttgtac
    14701 accggtaccc attgcgttga gcactttaat taaatcttta cctaactgcg gatggttgta
    14761 gttaacgaag cagccgtgaa agaaagcgac ctggtcttta tattgtgctt gctgagccgc
    14821 tatgctgcga taccagcgac ggaacgtgcc gaaggagtat ttcggtagcg tgcgacgatg
    14881 atcgattttt aacgccgcat caagcagctg ccgcaccggt ttcagcgagg tagcggtgtt
    14941 gacgattggt gcgaacggcg tggaaacgct acccatcagg tcggtatgac tcaacacaaa
    15001 attacgcagc gacgggcgcg tggtgtcata tttcgcccgc gcgcgctgga taatatcgcc
    15061 aatcttcaca tcggacgggc aggcgacttc acaacgtttg cagttgatgc aatatttcag
    15121 cgcctcgtca tacagtgcgc catctttcaa acgcagacgc tcgccatccg gcccggcttg
    15181 ttttggccct ggataaccgg gattcacccg gctcaccggg caggcggtgg tgcagacggt
    15241 gcacttaatg cagttttcga agctggtgtc gttcattgtt ggcctcctgc gcgttgggca
    15301 atctgttgag cggcatgtaa agcactgacg gcacaaacac cgccgccgca tccctgggcg
    15361 atgggatcaa atccgcccag caccgaaccg atggcaaaca ggttctcaat ggtttgccct
    15421 gcctgcgacg ggcgtagcgt ctcatcagtg gttacaccga actgctgcca cggttgcggc
    15481 gcaaaaaaat cgcccttata ccattcaccc cgcgtggcgg tttgtagcac atcaaggccg
    15541 agaatcggct cgcgaatgcc gttacgttcc gctactagtc cgccactaaa gaaactgccg
    15601 ctggcgagaa ccgcgaaacg tggacgtagc ggaatatcgg cgtgattgcg ggtccagatt
    15661 tcattcacta cgccattttt acaggtcact tttttcactt catcgcccgg catccacact
    15721 ccaccttggc gcacaaactg gcgctgtaac tggttttgca gacgaatgcc cagcacggaa
    15781 ggcggcagcg ttggcaaaag catcagtgaa caaggtagtt tttcattcaa ccaacgccac
    15841 agtttgtcat cggccaggcc gaagcaggcg ggcatcagga tcatttcgca ggtattggcg
    15901 acaggaataa gcgcatcaag taacagcggc cagttttctt cattatcaag gaaacgggcg
    15961 atattcaccg cgcgaaattc ggtggcgtta ttgcgcagga catccagttc cggcagctct
    16021 atttctgcgg tttcaaccga aagatccagt tcgcgcaacg aagccgccgc aagatgcgcc
    16081 tggaaatcca tcagcccgct tatccccact acacatatct tcttcgcggg tagcggccag
    16141 acgggaactt ctggcgaact taaccaggtt gcacgcaaag tgccgagcgg cgtaatccgc
    16201 tggtgagcca gttctacgct gccctgcaat tgcgcaccgc tttcagcgat taatgcctgc
    16261 gcctggcaag cgagatcgag cacgcgttgt ggcccgagaa gagagtaagg atgtgcaggt
    16321 gcctgctgac gcaaagattc cagtccactg tgaatgtctg ccacgggttg accatctggc
    16381 agatggctca gcaaatccag cgaaccggat gagaaatgca acgcgctttg accacgcgtg
    16441 acaatggcac agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa tccggcgagg
    16501 ccgccgccca taatgacagt atcaaagcgc atctttctgc tccttctcca gaccacacag
    16561 tccctgataa acccagcggg taaattcgct ttcgcgcagt gcgtctcccc aggcgatggg
    16621 ttgcacgcct ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga
    16681 cgtcgtgacg ttaaaccgtt gcagcagtcc ggcagcgcgg caggcgcaga gttcgccctg
    16741 gcaggtgccc atccccacac gggtacgacg gcgtaaatcc agcaggctat taacgtttaa
    16801 attttctacc gcgtactgca cttcacccgc agttaccgct tcgcactcac ataccaggct
    16861 acggtgtaga cggccttcgc tcagccaggc aggcgtgcga tcgccatgac gataaaccgc
    16921 agaaccgcgc agcggggcag gcagggagat gactttacgc aaggtaactt cagcgggttc
    16981 ttgtgaacca ggcagtgcca gatcggcagt cgtacagggg cgcgtgttgc ccagtttgcg
    17041 gcataccgcg tcggtagccc attcagccat cagccgatag gtcatcagtt tgccaccggt
    17101 gatggtgata aatccgtcca gaccatcgcg ttcagcatgg tcgagcagca cgatgccacg
    17161 gctgacgtta cgtccgctcg ggtcgtcatc gctggcaacc agcgggcgca cgccagaata
    17221 ggcccgcaaa atgcgcgttt tcgccatcac gggggccagt ttttcccctt cacgcagcag
    17281 aatatcaacc tcttctgccg tcactcgatt atcgtcaatc tcgttgtaat caatacgtaa
    17341 agaggtggta ccaatcagcg aaatggtatc gccaggcacc agaatatcgg cgtcggaagg
    17401 tttacggcag cggttgatca catgctggtt aatgcggtga tccatgatca gcagcgatcc
    17461 tttcgccggg aacatgcgaa tgcgcagatc ggcatattcg gcaatgtgtt gcccccagat
    17521 cccagcggca ttaaccacga caggtgcatg aagggcctga gtttcgccgg tgagatggtt
    17581 acgtacacga acaccgcaca ccgtcgcgcc ttcacgaatc agccccgtga cttcatgagc
    17641 ggtaaggata acggcaccgt gttctttggc atccagcatg tttgctgcgg tcagacgaaa
    17701 tggatcaacg gtgccatccg gaactttcac cgcgccaatc agtgccgggt taacggcagg
    17761 ttcgataatg cgcgcttgct gcgggtctat agcttctgcg ctgatccctg cttcttcgca
    17821 ggcgcgaata aaagtggcct ggaatgagag gtcatcttcc ggcagggtga taaacaggcc
    17881 gttggttggt tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt cactaatgca
    17941 ttcgcgggcc gattccgcat cggttactgc atagcgcgca ccgctgtgca gcaggccgtg
    18001 gttacgcccg gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc
    18061 gcgcagggca cagtcgcggg caatcccggc tcccgttgcg ccgccgccaa tgataatcac
    18121 gtcacttgat tgcgagtcgc gagttttcat tgttatccct ctgaagttcg ttttttacca
    18181 tttagccata gtaaaaacat gaattgtttg atttcgcgca tattcgctca tgattcgaaa
    18241 gtaaaacgtg atttcatgcg tcattttgaa cattttgtaa atcttattta ataatgtgtg
    18301 cggcaattca catttaattt atgaatgttt tcttaacatc gcggcaactc aagaaacggc
    18361 aggttctctc actgaatcag gctgttaatc ataaataaga ccacgggcca cggaggctat
    18421 caatgttgag tatttttaaa ccagcgccac acaaagcgcg cttacctgcc gcggagatcg
    18481 atccgactta tcgtcgattg cgctggcaaa ttttcctggg gatattcttt ggctatgcgg
    18541 cttactattt ggttcgtaag aactttgcgc ttgctatgcc ttatctggtt gagcagggat
    18601 tctcacgcgg tgatttaggt tttgcccttt cggggatctc gattgcttat ggattttcga
    18661 aattcatcat gggttcggta tcggatcgct cgaatccgcg cgttttcctg cccgcaggtt
    18721 tgattctggc ggcggcagtg atgttgttta tgggctttgt gccatgggcg acgtcgagca
    18781 ttgcggtgat gtttgtactg ttgttcctct gcggttggtt ccaggggatg gggtggccgc
    18841 cgtgtggtcg tactatggtg cactggtggt cgcagaaaga acgtggcggc attgtgtcag
    18901 tgtggaactg tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc ctgctgggga
    18961 tggcctggtt caatgactgg catgcggcgc tctatatgcc tgctttctgc gccattctgg
    19021 tggcattatt cgcctttgcg atgatgcgcg ataccccgca atcctgtggc ttgccgccga
    19081 tcgaagagta caaaaatgat tatccggacg actataacga aaaagcggaa caggagctga
    19141 ccgcgaagca aatcttcatg cagtacgtgc tgccgaacaa actgctgtgg tacatcgcca
    19201 tcgccaacgt gttcgtttat ctgctgcgtt acggcatcct cgactggtca ccgacttatc
    19261 tgaaagaggt taagcatttc gcgctggata aatcctcctg ggcctacttc ttctatgagt
    19321 atgcgggtat tccgggcacc ctgctgtgcg gctggatgtc ggacaaagtc ttccgtggta
    19381 accgtggggc aaccggcgtc ttctttatga cactggtgac catcgcgact atcgtttact
    19441 ggatgaaccc ggcaggtaac ccaaccgtcg atatgatttg tatgattgtt atcggcttcc
    19501 tgatctacgg tcctgtgatg ctgatcggtc tgcatgcgct ggaactggca ccgaaaaaag
    19561 cggcaggtac ggcagcgggc tttaccgggc tgtttggtta cctgggcggt tcggtggcgg
    19621 cgagcgcgat tgttggctac accgtggact tcttcggctg ggatggcggc tttatggtaa
    19681 tgattggcgg cagcattctg gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac
    19741 gtcgccatga acaattactg caaaaacgca acggaggcta atggcatgaa attgaagctg
    19801 aaaaacctta gcatggcgat catgatgagc actatagtca tgggaagcag tgcaatggcg
    19861 gcggacagca acgaaaaaat agtcatcgcc catcgcggtg ccagtggata tttgccggag
    19921 catacgctgc cagcaaaagc gatggcgtat gcgcagggag cggattatct ggaacaggat
    19981 ttggtgatga ccaaagacga ccatctggtt gttctgcatg accattatct cgatcgtgtt
    20041 actgatgttg ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata
    20101 gatttcacgc tggatgaaat taagtcgctg aaatttaccg aaggtttcga tattgaaaac
    20161 ggtaaaaaag tacagactta tccggggcgt ttcccaatgg gtaagtccga cttccgggtg
    20221 cacacctttg aagaagagat tgaatttgtt caggggttaa atcactctac cgggaaaaat
    20281 atcggtatct atccagaaat caaagcgccg tggttccatc atcaggaagg gaaggatatt
    20341 gcggcaaaaa cgctggaagt gctgaagaaa tatggttaca ccggtaaaga cgataaagtt
    20401 tatttgcaat gttttgatgc tgatgagctg aagcgtatta agaatgagct ggaacccaaa
    20461 atgggcatgg agctcaatct ggtacagctg attgcctata ccgactggaa tgaaacgcag
    20521 cagaaacagc cggatggaag ctgggttaat tacaactacg actggatgtt taagccggga
    20581 gccatgaaac aggtggcgga atatgcagat ggtattggtc cggattacca tatgttgatt
    20641 gaggagacat cgcagccggg taatatcaaa ctcactggca tggtgcaaga tgcccagcag
    20701 aataaactgg tagtgcatcc ttataccgtg cggtcagata aactgcctga atacactcct
    20761 gatgtgaatc agttatatga tgctctgtat aacaaagcgg gtgtaaatgg gctgtttact
    20821 gatttccctg ataaggcagt aaaatttctt aataaagagt aatgttactg caggatggcg
    20881 ttcatgcgcc atcctgtctc atatcaaatt tttccaatcg ccactaattg ttccatgcac
    20941 atttgtagta aaaacagtgg actgccttct accggctttt ctttagtcag caacgaaatc
    21001 tgtcgataga aggttggttc gtgaggaacg gaacacaatt cacctgcaat gcttttcagc
    21061 gttaactctg gttgtaaggc gatgcctaat ccctgtcgaa taaaattcat cgctgtatcc
    21121 gactgattaa attcacattt tatctgctgg gcgcactatt agtggtagtg gctggtggaa
    21181 tctatgcgct gcaaatgaag ggcaaaagcg gtgtcacacc tgtagcggca aaagaaattc
    21241 attaatacga cacgtttcat taagattttc ctcaggtaac cacctatagt cattgtcgtt
    21301 aataaaaatg acaggcgata gggtatggca gtttcagcaa agtacgacga atttaatcac
    21361 tggtgggcaa cagagggcga ctgggttgaa gagcctaact atcgccgtaa tggtatgagc
    21421 ggggtgcaat gcgtcgagcg caacggcaaa aagctgtatg taaagcgcat gacgcatcat
    21481 ctgtttcatt cagtacgtta tccgttcggc cgaccaacga ttgtccgtga ggttgcggta
    21541 attaaagaac tggaacgggc cggtgtcatc gtgccgaaga tcgtttttgg tgaagcggtg
    21601 aaaattgagg gtgaatggcg cgcgttgctg gtgactgaag atatggcggg gttcatcagc
    21661 attgctgact ggtatgcccg gcatgcagta tcgccttatt ctgacgaagt acggcaagcc
    21721 atgttgaaag cagtggcact ggcgtttaag aaaatgcata gcgttaatcg tcagcatggc
    21781 tgttgctatg ttcgccatat ctatgtgaaa acagaaggta aggcagaagc tggttttctt
    21841 gatctggaga aaagtcgtcg ccgcttgcgt cgcgataaag cgattaatca tgacttccgc
    21901 cagctggaaa aatatctgga gccgatccct aaagcggact gggagcaggt gaaagcgtat
    21961 tactacgcca tgtaatcgtt gtaggccaga taatacgcgt aagcgtcgta tctggcgcta
    22021 gtacccgatc acaactcgat ttcaatatcg ccttttgccc ggcaacaaca gggcaaaatt
    22081 tcccccggct gaataaaggc taacggttcg gcaatccagt ccacctgacc tgcgaccagt
    22141 cgggtgcgac aggagccgca gtaaccttcg cggcactggt actcaaccgc cacattgtgg
    22201 gattccagcg ccgccagaag ggaagggtgt tcatcctggc acagcagttg tgtgccagtg
    22261 atgcgcaggg taacgcgggc catcagagct ggaagttact caaatcgtcg gtgtccactt
    22321 ccgcgtcaat ctgcccgacc agataagaac tgacttccac ttcctgcgga gcaacctgca
    22381 cgttatcaga caccagccag gtgttgatcc acgggatcgg gttggagcgc gtctggaacg
    22441 gcagatccaa accgactgcc tgcatgcgga tattggtgat gtattcaacg tactggcaga
    22501 gaatatcttt attcagacca atcatcgaac cgtcacggaa cagataatcc gcccagtctt
    22561 tctcttgctg tgccgcctgg acaaacaggt catagcactc ctgcttacat tcttcggcaa
    22621 tttccgccat ctccggatca tccgcgccgc tgcgcagcag attcagcata tgctgagtac
    22681 cggtcaggtg cagggcttca tcgcgggcaa tcaggcgaat aatcttggcg ttaccttcca
    22741 tcagttcgcg ttctgcaaag gcaaaggaac aagcaaagct gacgtagaag cggatcgctt
    22801 ccagcgcgtt aacgctcatc aggcagagat acagtttttt cttcagctcg cgcaggctaa
    22861 cggttacggt tttaccgtta acggtgtggg tgccttcgcc cagaaggtgc cagtagctgg
    22921 tcatttcgat cagttcatcg taatagctgg agatcccttc cgcacgtttc tggatctgct
    22981 cgttggtgac gatatcgtca aacacaacag acggatcgtt aacgatatta cgaatgatat
    23041 gagtatagga acgggaatga atcgtttctg agaacgccca ggtttcgacc caggtttcca
    23101 gttccggaat agaaataagc ggcaatagcg ccacgttcgg gctacgaccc tgaatggaat
    23161 ccagcagcgt ctgatatttc aggttgctga taaagatgtg tttttcgtgc tccggcagcg
    23221 cctggtaatc tatacggtcg cgggagacgt caacttcttc cggacgccag aagaaagaga
    23281 gctgcttttc gatcagcttt tcgaagatgt catatttttg ctgatcgtag cgagccacgt
    23341 tgaccggctg accaaagaac atcggttctt tgagctgatc atttttcgtc tgtgaaaagg
    23401 tggtatatgc catgagtgtg tcctgttggg agtttaatgc cggataaggc attttacgcc
    23461 gcatcctgtg cctgatgcga cgctggcgcg tcttatcagg cctacaaatc cgagccgtag
    23521 gccggataag gcgttcacgc tgcatccggc acccggagcc tgatgcgacg ctggcgcgtc
    23581 ttatcaggcc tacaaaccga gccgtaggcc ggataaggcg tttacgccgc atccggcatc
    23641 tcaatatcag atcttacatg cgccgctttc gcagccatcg tcctggattg acggtaccag
    23701 atcgtcctgc gcgtcttcag cgccgtcacg ggtgttctga taatacagcg ttttgacccc
    23761 gaatttgtag gcggtgagca ggtctttcag caactgctgc atcggcactt ttcctgacgg
    23821 gaaacgtgac ggatcgtagt tggtgttagc agagatcgac tgatcgataa atttctgcat
    23881 gatacctacc agttgcaggt agccatcgtt acccggcatt tcccacagca gctcataggc
    23941 gtcgtgcagg tgctcgtagt ccggcaccac ctggcgcaaa ataccgtctt tcgacgcttt
    24001 gatgctgacg taaccgcgcg gcggttcaat accgttagtg gcgttagaga tctgcgaaga
    24061 ggtctcggac ggcatcaggg cagaaagcgt ggagttacgc aggccgtgcg ttttgattga
    24121 ttcacgcaat gcttcccagt cgtaatgcag cggctcatta gcgatggcat ccagatcttt
    24181 cttataggta tcgatcggca ggatcccttt cgcgtaagtg gtttcgttaa accacgggca
    24241 cgcgccttgc tctttagcca gctcattaga cgctttcagc aggtaatact gaatcgcttc
    24301 gaaggtttta tgcgtcaggt tgttggcgct gccgtcggag tagcgtttac cgtgcttcgc
    24361 cagatagtaa gcgaagttaa tcacaccaat acccagcgta cgacgcccca tcgctccacg
    24421 tttggcggcc gggatcgggt aatcctgata atccagtagc gcgtcaagtg cacgaaccgc
    24481 cagaatcgcc agctcttcca gttcatccag gctattaatt gcgcccaggt tgaaagcaga
    24541 cagcgtacac agcgcgattt caccgttctc gtcgttgacg tcgttcagcg gtttggtcgg
    24601 cagggcaatt tctaggcaca ggttagactg gcgaactggc gcgatggtcg gatcaaacgg
    24661 actatgggta ttacagtggt caacgttctg aatatagata cgaccggtag acgcacgttc
    24721 ctgcatcatc agcgagaaca gctcaacggc tttcacacgc tgcttgcgga tgctgtcgtc
    24781 tttctcatat ttggtataca ggcgttcaaa ctcttcctga tcggcgaaga acgcgtcgta
    24841 cagccccggt acgtcggacg ggctgaacag ggtgatatct tcacctttca gcaggcgggt
    24901 atacatcagt ttgttgattt gtaccccgta gtccatatga cgcacgcggt tgccttcaac
    24961 cccacggttg tttttcaaca ccagcaggct ttccacttcc agatgccaca tcgggtagaa
    25021 cagcgttgcc gcaccgccgc gcacaccgcc ctgagagcag gatttcaccg ctgtctggaa
    25081 atgtttgtag aacgggatac agccggtatg gaacgcttcg ccaccgcgaa tcgggctgcc
    25141 cagcgcacga atacgtccgg cgttgatgcc gatcccggca cgctgggaaa catatttcac
    25201 aatcgcgctg gaggtggcgt tgatggaatc caggctgtca ccgcactcga tcagtacgca
    25261 ggagctgaac tgacgagtcg gggtacgcac gccggacatg attggcgtcg gcagcgaaat
    25321 tttaaatgtg gaaaccgcgt cataaaaacg cttcacgtat tgcaggcgcg tttcacgcgg
    25381 gtagttcgag aacaggcacg cggcaacaag aatataaagg aactgggcgc tttcgtagat
    25441 ttcgccggtc acgcggttct gtactagata tttgccttcc agctgcttaa cggcagcata
    25501 agagaaggtc atatcacggt cgtgatcgat aaacgtgtcc atctgcttga actcttcttc
    25561 cgtgtagtct tccagcagat gattatcgta tttgcccatc tcgaccattt tcaccacgtg
    25621 gtcgtacagc gcaggcggct caaactggcc gtaggctttt ttacgcaggt ggaagatcgc
    25681 caggcgcgcg gcgagatact gataatccgg cgcatcacgg gagatcaggt ctgcggcagc
    25741 cttgataata gtttcgtgga tgtcagaggt cttgataccg tcataaaact gaatgtggga
    25801 gcgcagctcg acctgggaaa tcgaaacgtt atgcagtcct tctgccgccc aatccagaac
    25861 gcgatggatt ttgtcgagat tgatgcgctc tgtgctaccg tcgcgctttg tcaccagcag
    25921 attctgattc atgtatgtcg tacctgtttt tggaattctt tccgcaatag tttcatgcag
    25981 actacctgta gtaagggaag ctgcttagaa cactatatat agggggtatg tttgggattc
    26041 actgcaagat agtgtgaaaa tgaccctctt gcaagtgcat aactttgtgg ataactcagg
    26101 aaggaaaaag tggctttcgc gcaccttagg tcagacaagg tgtccgggaa agtcaatggg
    26161 aagagaaaaa tttgttaaaa ataactgttc ggcgatttct tgaacggcag aataggttgg
    26221 ataaattgat atagatggtt tattaatctt tttgctaatg attgcaaaaa ttgatctaag
    26281 cacgagtcat cacataatct gatgtatcag ttgtggtcaa tatataagcg aatgtaagcg
    26341 attgttgatg tcgaaatggt tgttttctat tcggttattg ttaaatgcga agccattttg
    26401 ttaatttacc ttatattata agaaaattat tcattacttt aatttagttt aaatataact
    26461 ggtatccctt aaggttatta acttgaatta tgacgcactg atattattca tcaaataata
    26521 acaaaatagc cattgcatcg ggttggacaa tttacaaaag aaagataacc catattgtat
    26581 aatgcgttta aatatgccgt cttatataaa atgatggtca gattaatatg cggattatct
    26641 ttctacgcaa ggagtattta tctctactcc cgtcaatgat tgcatctctt ttctctgcta
    26701 acggtgtcgc ggcggccatt gatttatgcc agggatatga tatcaaagcg agttgtcacg
    26761 ccagcaggca aagcctttca ggcattacg
//
LOCUS       DAJTMX010000047        26760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTMX010000047 DAJTMX010000000
VERSION     DAJTMX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.047"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO145_001965"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO145_001965"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5467092.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO145_001966"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO145_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5467093.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO145_001967"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO145_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5467094.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO145_001968"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO145_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5467095.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO145_001969"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO145_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5467096.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO145_001970"
     CDS             5377..5835
                     /locus_tag="OO145_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5467097.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO145_001971"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO145_001971"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5467098.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO145_001972"
     tRNA            6887..6971
                     /locus_tag="OO145_001972"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO145_001973"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO145_001973"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO145_001974"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO145_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5467099.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO145_001975"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO145_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5467100.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO145_001976"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO145_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5467101.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO145_001977"
     CDS             complement(10252..13995)
                     /locus_tag="OO145_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5467102.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO145_001978"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO145_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5467103.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO145_001979"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO145_001979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5467104.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO145_001980"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO145_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5467105.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO145_001981"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO145_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5467106.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO145_001982"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO145_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5467107.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO145_001983"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO145_001983"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5467108.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO145_001984"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO145_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5467109.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO145_001985"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO145_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5467110.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO145_001986"
     CDS             23392..23499
                     /locus_tag="OO145_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5467111.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO145_001987"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO145_001987"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5467112.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO145_001988"
     CDS             complement(24538..25347)
                     /locus_tag="OO145_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5467113.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO145_001989"
     CDS             complement(25351..25743)
                     /locus_tag="OO145_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5467114.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO145_001990"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO145_001990"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5467115.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..>26760)
                     /gene="prfA"
                     /locus_tag="OO145_001991"
     CDS             complement(26573..>26760)
                     /gene="prfA"
                     /locus_tag="OO145_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="recognizes the termination signals UAG and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF1; this protein is
                     similar to release factor 2; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5467116.1"
                     /translation="SGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEP
                     IIQEHQADQLAALSEQE"
BASE COUNT         6730 a         7013 c         6437 g         6580 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
//
LOCUS       DAJTMX010000048        26457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTMX010000048 DAJTMX010000000
VERSION     DAJTMX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.048"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            47..154
                     /locus_tag="OO145_001992"
     CDS             47..154
                     /locus_tag="OO145_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5467117.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            530..637
                     /locus_tag="OO145_001993"
     CDS             530..637
                     /locus_tag="OO145_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5467118.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(724..2403)
                     /gene="bcsG"
                     /locus_tag="OO145_001994"
     CDS             complement(724..2403)
                     /gene="bcsG"
                     /locus_tag="OO145_001994"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5467119.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2400..2591)
                     /gene="bcsF"
                     /locus_tag="OO145_001995"
     CDS             complement(2400..2591)
                     /gene="bcsF"
                     /locus_tag="OO145_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5467120.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2588..4159)
                     /gene="bcsE"
                     /locus_tag="OO145_001996"
     CDS             complement(2588..4159)
                     /gene="bcsE"
                     /locus_tag="OO145_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5467121.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4432..4620
                     /locus_tag="OO145_001997"
     CDS             4432..4620
                     /locus_tag="OO145_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5467122.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4632..5384
                     /gene="bcsQ"
                     /locus_tag="OO145_001998"
     CDS             4632..5384
                     /gene="bcsQ"
                     /locus_tag="OO145_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5467123.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5381..5740
                     /locus_tag="OO145_001999"
     CDS             5381..5740
                     /locus_tag="OO145_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5467124.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5737..7998
                     /gene="bcsA"
                     /locus_tag="OO145_002000"
                     /pseudo
     CDS             5737..7998
                     /gene="bcsA"
                     /locus_tag="OO145_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8009..10348
                     /gene="bcsB"
                     /locus_tag="OO145_002001"
     CDS             8009..10348
                     /gene="bcsB"
                     /locus_tag="OO145_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5467125.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10355..11461
                     /gene="bcsZ"
                     /locus_tag="OO145_002002"
     CDS             10355..11461
                     /gene="bcsZ"
                     /locus_tag="OO145_002002"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5467126.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11538..11879
                     /locus_tag="OO145_002003"
     CDS             11538..11879
                     /locus_tag="OO145_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5467127.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11794..12045
                     /locus_tag="OO145_002004"
                     /pseudo
     CDS             11794..12045
                     /locus_tag="OO145_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            12127..14115
                     /gene="hmsP"
                     /locus_tag="OO145_002005"
     CDS             12127..14115
                     /gene="hmsP"
                     /locus_tag="OO145_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5467128.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14303..15589
                     /gene="dctA"
                     /locus_tag="OO145_002006"
     CDS             14303..15589
                     /gene="dctA"
                     /locus_tag="OO145_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5467129.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15810..17306
                     /locus_tag="OO145_002007"
     CDS             15810..17306
                     /locus_tag="OO145_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5467130.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17402..18331)
                     /gene="kdgK"
                     /locus_tag="OO145_002008"
     CDS             complement(17402..18331)
                     /gene="kdgK"
                     /locus_tag="OO145_002008"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5467131.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18563..19330
                     /gene="pdeH"
                     /locus_tag="OO145_002009"
     CDS             18563..19330
                     /gene="pdeH"
                     /locus_tag="OO145_002009"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5467132.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19400..21460
                     /locus_tag="OO145_002010"
     CDS             19400..21460
                     /locus_tag="OO145_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5467133.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21642..22964)
                     /locus_tag="OO145_002011"
     CDS             complement(21642..22964)
                     /locus_tag="OO145_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5467134.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23375..24388)
                     /gene="yhjD"
                     /locus_tag="OO145_002012"
     CDS             complement(23375..24388)
                     /gene="yhjD"
                     /locus_tag="OO145_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5467135.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24437..25336)
                     /locus_tag="OO145_002013"
     CDS             complement(24437..25336)
                     /locus_tag="OO145_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5467136.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25856..>26457
                     /locus_tag="OO145_002014"
     CDS             25856..>26457
                     /locus_tag="OO145_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5467137.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6314 a         6640 c         7213 g         6290 t
ORIGIN      
        1 ctcaagaata gccgcgaaac gttgtcatta caacacaggc ggctatatga cgctcgcaga
       61 actgggcatg gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag
      121 tatgatcgtg aactggctga acaagcggaa gtaacgtgtc atgcgggcgt caggctgccg
      181 taatggcaat ttgcgcccgg accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa
      241 gaaaaatgca acgattgcac gcggataata ttctgcggaa tgcgcagcac agagtgaaga
      301 cgtgacggat tccggaacag aaaaacagtc gtagaatcag cggcaagccg gaaaggttcc
      361 ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa
      421 acccccgcac gttgcaggta tgcacctgac aacatcacgg gggctaatct tgaacccaaa
      481 caactcaaga atagccgcga aacgttggca ttacaacaca ggcggctata tgacgctcgc
      541 agaactgggc atggccttct ggcatgattt agcggctccg gtcattgctg gcattcttgc
      601 cagtatgatc gtgaactggc tgaataagcg gaagtaacgt gtcatgcggg cgtcaggctg
      661 ccgtaatggc aatttgcgcc cggatcaggc cgcagaggtt aaactctgcg gcctttttcg
      721 tgcttactgc gggtaaggca cccagtcgcc gccgttcagg cgaacgtacg gtttatcttg
      781 gtattgaata actactgcat ttgagttctc ggagaccggt gctgtttgtg gcaacccact
      841 ggtgagtttt ttccagtcaa cattgtcttc ggtgaaaatc ttgccatcaa gaacgcgaac
      901 caccagatcg gagatagcca ggaagctgct cggttggtca atgacaatcg gtgccccctg
      961 atgtggtgcc ttcatgccga agaatttcac cccaacgggg acgtctgtga tagacgggct
     1021 agggatatca cgtaggccag atacctgcat tctgtcgccc ttcagcgcgc cgccgtgttc
     1081 cggcaccacg accaccatca ctttacgacc cgatttctcc agttcagtaa agaaggcgtc
     1141 cagttcatca aagaatttct gcgcccgcgc tttgtaatcc gctgttttgc tgacccccgg
     1201 ataatggttg ccgtcatgca gtggaagcgt gttgtagaac gtggcactac ggctgttttt
     1261 atctttttcg gtaacgtcca gccagcggtt aagcacggcg gtatcgtcat aaaccggcga
     1321 accatcaaag cccagcaaaa taaccggcag atttgtttga tccatcaatt cagtctgcat
     1381 gccgccattt tcgcgaactt ctttcaaaaa accgccgaac tggccgttat gtcccatcat
     1441 caggtgctgg gtaaagccca gtttcgaaag gttatcaaac agatagcagt cgttatttgc
     1501 cggttgatac agattagtgt gcgaagtctg cccgcagctg gcgcgcagta aacggatcgc
     1561 cgccgggcca ctgtaggagg tggcggagtt aaagttcttg aaatcaatat cgaaatgcga
     1621 ccacagtgga tgcgacatca acccggcggc ttctatatcc gaccaggaaa gcgaacagat
     1681 gttaatcacc agtagttcaa atggctgagc atcagcgggc agcgaagacg ggaaggtcga
     1741 tttacgtttc gcctccgcgt tatagaaatt attcagccag gcgttaaggt tcgccgttgt
     1801 tggcggtgca gtttgtgcgg gcatatcacc cactaccggt gcgccacccg tcgccgcaac
     1861 ggttgccgct gcgttaccac ccgtcgttgt tacagtggtc gtcggttgtc cggctggcca
     1921 caaggagaaa cttggtcccg ccagggtaag tacgttcagc catagcagta tggcaaccac
     1981 aaaaacggta atgcgaatcc attgtgacag gaataaccag gccactaata aaacaaaaat
     2041 ggccccaatc atctgccagt taataaagcg tgtgacaagg tcgattaaat aatcggtact
     2101 gaaccccgcc acctgcgaac cctggctcat tatgctttcc gggccaggca accagatgtc
     2161 atgccagaac aaagcaaagc cgatcggcag ggcaatccag tggcgcaagc gatgcaggct
     2221 gtaacgcgga atgggcatca gcagaaacgc ggcaaacacc aaattgagga gcgggtggaa
     2281 gttaagatat cccgcccaca acaggccaaa cttaaccaga aaatagaagt tccagccgga
     2341 aaggccgcgc cagtattgcc agagggaaga aggcatggcg gtattttgcg taaattgagt
     2401 cattttttgg ttgccctggc tttttccgtg cggcgtaacg tcccggccgg tttaacataa
     2461 cgaggtttag caaagaacaa acgtaaggtg tcgcgaatgc gtcgcaaaga gtgccgcgcc
     2521 agatagccca gcgggaaaaa tatcagtgcg caaacgataa taatttcaat gatatcgctg
     2581 atggtcatca tgatgagcgc tccacagcat catctaacag tcgcatgggt tctggtattc
     2641 gtcgccagtg gcgaccatcg tgctcggcat tgatgaccgg tttagaactt tgcgttaaag
     2701 gcagcggcat tccccattgt tctggggcaa gcaggcgcat ctgcaccagc tcggcactga
     2761 tttgatcatc ttcaaaccag accatacggt ttgagaaaat gtcgccagta ggcaatggga
     2821 aaatatgatt caacgcggta tccagatcgt taatccgaca gaatgagaga aacagcacca
     2881 gccgattacc gccaatggtc atgatatcgc cggtacggtt agggcgacat agcgtcaggg
     2941 cttgctcaac gcggataccc ggtaccggac gtagggcaac cagaacgcct ttaccgtggg
     3001 caggcaagag agggttattc atcatgttgt tgacggcatt acagaacaca tcccacttct
     3061 ggaaaccacg cagtttgagc ggctgggtca ttgacagcaa ggtagtgata tcttccggta
     3121 catagcgact aaacttctgc ccttgcacgc tttcgatcat cgtcagacaa cgggagagtg
     3181 gcgcattcca cggaataacc atatttgcac cgcaggccaa taacaaacgt tcatcggtgg
     3241 cgcgcaggct agcggtattt tcccgcacga ggattttcat cgcactaccg cgctggcgac
     3301 gcagggtatg aatgctgcgg gccagtggct cgatttgcgc attttgctgt aaagaaaaga
     3361 ccaccgtcgc cgcctgagcg gtacgggctt cattgaacag gacttcgtta ttgttgaaca
     3421 gttgccagtg ttccgatagc ggcggcgcac cttccagtac agcaacatta ctcagaatgc
     3481 gtttttcgtc gctgcgtggt tggatctccg cctcttcgct ttgaactaat gtccagatac
     3541 cattttgttg ctgaacgcta agctgctgac gggcgctgac ccctttttcg ttgcaccaga
     3601 aggcaatatc cagcaaatgt tggtcaccct gaaaacgcaa actggcaaga ccaaaaagtg
     3661 aacggtattc ctcaagcaac aatgaaaatt gtttatcgtt attatttccg ggattaatta
     3721 ccaaaagcga acaatggttt aacctgctcc atttattcat tttatccaac catgagcgaa
     3781 gccgctcggc aggaatattt tgccatgcgt tatttgcgca aacaagaata aaaaggtaat
     3841 tatggggatc aatagaacac tgcaaatcac gggccaaata gtatagacct ttttcatgat
     3901 ttagcattga aaataatttt attttttccg gcccttgaga atcatctaat tgaaagattt
     3961 tcgccggatc gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc tgggatgcaa
     4021 ttgtttgatt cgccagactg atagcatctt catggcgatc gacgttaaac caccagacgc
     4081 cgcctgctgg catatgtcgc agctcatccc ataatgatga gataccgata gagaatacag
     4141 ggtccacaat gtccctcatg tcaccatttt gtctgattat cagtttacta gcgaaagcta
     4201 agaaataaac ctaccattga aattaaaact tatcagattt agcatgtgaa tcaatttaga
     4261 aggtcacaag acgtgtgatt ttagtctttt cgtttttaac tttttctgtt gcgtaacgga
     4321 aagtcaaaaa gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg aaatgctcac
     4381 ggttagaatt gtctcatgaa tggtacggtt atttcatagg gatcaagcaa aatgaataac
     4441 aatgaaccag atactctgcc tgatcccgcg ataggctata tcttccagaa tgatattgtg
     4501 gcgttaaagc aggcattttc actgcctgat attgattatg ccgatatttc ccaacgcgaa
     4561 cagttggccg cggcattaaa acgctggccg ttgctggcag agtttgcgca acaaaagtag
     4621 gggattggtg aatggccgta ctgggattgc agggggtgcg gggaggcgtg gggacaacaa
     4681 ccatcaccgc cgcattagcc tggtcattac aaatgttggg agaaaatgtc ctggtggtcg
     4741 atgcctgccc ggacaacttg ttgcgcctgt catttaatgt tgattttacc caccgtcagg
     4801 gctgggccag agcgatgctg gatggccagg actggcgtga cgctgggttg cgctacacct
     4861 cgcagctcga tttgctgcct tttggtcagt tatccattga agaacaagaa aatccacagc
     4921 actggcaaac ccggctgagc gatatttgct ccggcttaca gcaactaaaa gccagcgggc
     4981 gttaccagtg gattttaatc gacttaccgc gtgatgcctc gcagataacc caccagctgc
     5041 tgagtttgtg cgagcactcg ctggcaatcg tcaatgtgga tgccaactgc catatccgac
     5101 tgcatcagca agcgctgccg gatggcgcac atattttgat taatgacttc cgtattggca
     5161 gtcaggttca ggacgatatt taccagcttt ggttgcaaag ccagcgccga ttactgccga
     5221 tgctcattca tcgtgatgaa gcgatggctg aatgcctggc ggctaagcaa ccagtaggtg
     5281 aatatcgcag tgatgcgctg gcggctgaag agatactgac gctggcgaac tggtgcctgt
     5341 tgaactactc cgggctgaaa acgccagtcg ggagtgcatc atgagtatcc tgacccggtg
     5401 gttgcttatc ccgccggtca acgcgcggct tatcgggcgt tatcgcgatt atcgtcgtca
     5461 cggtgcgtcg gctttcagcg cgacgctcgg ctgtttctgg atgatcctgg cctggatttt
     5521 tattccgctg gagcacccgc gctggcagcg tattcgcgca gaacataaaa acctgtatac
     5581 gcatatcaac gcctcgcgtc cgcgtccgct ggacccggtc cgttatctca ttcaaacatg
     5641 ctggttattg atcggtgcat cgcgcaaaga aacgccgaaa ccgcgcagcg ggcattttca
     5701 ggtctgcaaa atattcgtgg acgttaccat caatggatga acgagctgcc tgagcgcgtt
     5761 agccataaaa cacagcatct ggatgaaaaa aaagagctcg gtcatttgag tgccggggcg
     5821 cggcggttga tcctcggtat catcgtcacc ttctcgctga ttctggcgtt aatctgcgtt
     5881 actcagccgt ttaacccgct ggcgcagttt atcttcctga tgctgctgtg gggggtagcg
     5941 ctgatcgtac ggcggatgcc ggggcgcttc tcggcgctaa tgttgattgt gctgtcgctg
     6001 accgtttctt gccgttatat ctggtggcgt tacacctcta cgctgaactg ggacgatccg
     6061 gtcagcctgg tgtgcgggct tattctgctc ttcgctgaaa cgtacgcgtg gattgtgctg
     6121 gtgctcggct acttccaggt agtatggccg ctgaatcgtc agccggtgcc attgccgaag
     6181 gatatgtcgc tgtggccgtc agtggatatc tttgtcccga cttacaacga agatctcaat
     6241 gtggtgaaaa ataccattta cgcctcgctg ggtatcgact ggccgaaaga caaactgaac
     6301 atctggatcc tcgatgacgg cggcagggaa gagtttcgcc agtttgcgca aaacgtgggg
     6361 gtgaaatata tcgcccgcac cactcatgaa catgcgaaag cgggcaacat caacaatgcg
     6421 ctgaaatatg ccaaaggcga gttcgtgtcg attttcgact gcgaccacgt accaacgcga
     6481 tcgttcctgc aaatgaccat gggctggttc ctgaaagaga aacagctggc gatgatgcag
     6541 actccacacc acttcttctc gccggacccg tttgaacgca acctgggacg ttttcgtaaa
     6601 acgccgaacg aaggcacgct gttctatggt ctggtgcagg atggcaacga tatgtgggac
     6661 gccactttct tctgcggttc ctgtgcggtg attcgtcgta agccgctgga tgaaattggc
     6721 ggcattgctg tcgaaaccgt gactgaagat gcgcatactt ctctgcgttt gcaccgtcgt
     6781 ggctatacct ccgcgtatat gcgtattccg caggcggcgg ggctggcgac cgaaagtctg
     6841 tcggcgcata tcggtcagcg tattcgctgg gcgcgcggga tggtataaat cttccgtctc
     6901 gataacccgc tcaccggtaa aggactgaag tttgctcagc ggctgtgcta cgtcaacgcc
     6961 atgttccact tcttgtcggg cattccacgg ctgatcttcc tgactgcgcc gctggcgttc
     7021 ctgctgcttc atgcctacat catctatgcg ccagcgttga tgatcgccct attcgtgctg
     7081 ccgcatatga tccatgccag cctgaccaac tcgaagatcc agggcaaata tcgccactct
     7141 ttctggagtg aaatctacga aacggtgctg gcgtggtata tcgcaccacc gacgctggtg
     7201 gcgctgatta acccgcacaa aggcaaattt aacgtcaccg ccaaaggtgg actggtggaa
     7261 gaagagtacg tcgactgggt gatctcgcgg ccctacatct tccttgttct actcaacctg
     7321 gtgggcgttg cggtaggcat ctggcgctac ttctatggcc cgccaaccga gatgctcacc
     7381 gtggtcgtca gtatggtgtg ggtgttctac aacctgattg ttcttggcgg cgcagttgcg
     7441 gtatcggtag aaagcaaaca ggtacgccga tcgaaccgcg tggagatgac gatgcccgcg
     7501 gcaattgccc gcgaagatgg tcacctcttc tcgtgtaccg ttcaggattt ctccgacggt
     7561 ggtttgggga tcaagatcaa cggtcaggcg cagattctgg aagggcagaa agtgaatctg
     7621 ttgcttaaac gcggtcagca ggaatacgtc ttcccgaccc aggtggcgcg cgtgatgggt
     7681 aatgaagttg ggctgaaatt aatgccgctc accacccagc aacatatcga ttttgtgcag
     7741 tgtacgtttg cccgtgcgga tacatgggcg ctctggcagg acagctaccc ggaagataag
     7801 ccgctggaaa gtctgctgga tattctgaag ctcggcttcc gtggctaccg ccatctggcg
     7861 gagtttgcgc cttcttcggt gaagggcata ttccgtgtgc tgacttctct ggtttcctgg
     7921 gttgtatcgt ttattccgcg ccgcccggag cggagcgaaa cggcacaacc atcggatcag
     7981 gctttggctc aacaatgatg ataacgcgat gaaaagaaaa ctattctgga tttgtgcagt
     8041 ggctatgggg atgagtgcgt tcccctcttt catgacgcag gcgacgccag caacgcaacc
     8101 actgatcaat gctgagccag ctgtagccgc ccagacggaa caaaatccgc aggtggggca
     8161 agtgatgtca ggcgagcagg gagctgatgc ccccatcgtg gcgcagaacg gtccttcgcg
     8221 tgatgtgaag ctgacctttg cgcaaatcgc gccgccgccg ggcagcatgg tgctacgtgg
     8281 tattaacccg aacggcagca ttgagtttgg tatgcgcagc gatgaagtgg tgacgaaggc
     8341 gatgctcaac ttcgaataca cgccatcgcc atcgttactg cctgtccagt cgcagttaaa
     8401 ggtttatctc aatgatgagc tgatgggcgt gctgccagtg accaaagaac agttgggtaa
     8461 aaaaacgctg gcgcaaatgc ccattaatcc actgtttatt accgacttca accgtgtgcg
     8521 gctggagttt gtcggccatt atcaggacgt gtgcgaaaac ccggccagca ccacgctttg
     8581 gctggatgtt gggcggagca gtggactgga tctgacctat cagaccctga atgtgaagaa
     8641 tgacctgtca cacttcccgg tgccattctt tgacccgcgc gataaccgta ctaacacctt
     8701 gccgatggtc tttgcgggtg cgccggatgt tgggctgcaa caagcatctg ccattgtcgc
     8761 ctcgtggttt ggttcgcgtt ctggctggcg tgggcagaac ttcccggtgc tctataacca
     8821 attgccggat cgcaatgcca ttgtctttgc aaccaacgac aaacggccgg acttcctgcg
     8881 cgatcatccg gcggtaaaag ccccggtgat tgagatgatt aaccatccgc agaatcctta
     8941 cgtcaaactg ctggtggtgt ttggtcgtga cgacaaagac ctgttgcagg cagcgaaagg
     9001 tatcgctcag ggtaacattc tgttccgtgg tgaaagcgtg gtagtgaatg aagtgaaacc
     9061 gctgctaccg cgtaagccgt acgatgcgcc gaactgggta cgtaccgatc gtccggtcac
     9121 atttggcgaa ctgaaaacct atgaagaaca gttacaatcc agcggccttg agccagcagc
     9181 gattaacgtt tcgctaaacc tgccgccgga tctctacctg atgcgcagta ccggcattga
     9241 tatggatatt aattaccgct acaccatgcc gccggtgaaa gacagttcgc ggatggatat
     9301 cagcctgaat aaccagttcc tgcaatcctt caacctgagc agcaaacagg aggcgaaccg
     9361 tctgctgctg cgtattccgg tattacaagg tttgctggat ggcaaaacag atgtctctat
     9421 tccggcgctg aaactgggcg cgaccaacca gctgcgcttc gactttgagt atatgaaccc
     9481 gatgccaggc ggttcggtag ataactgtat taccttccag ccggtgcaga atcatgtggt
     9541 gattggtgac gattccacca tcgacttctc gaagtattac cacttcatcc cgatgccgga
     9601 tctacgcgcc tttgctaacg cgggcttccc attcagccgg atggcggatc tgtcgcaaac
     9661 catcaccgtg atgccgaaag cgcctaacga agcacagatg gaaacgttgc tgaatactgt
     9721 tggttttatc ggcgcacaga cgggcttccc ggcgattaat ctgacggtga ccgatgatgg
     9781 cagcaccatt cagggcaaag atgccgacat catgatcatc ggtggtatcc cggacaaact
     9841 gaaagacgat aagcagatcg acctattggt gcaggcgacc gaaagctggg tgaaaacacc
     9901 gatgcgccag accccgttcc ccggcattgt gccggacgag agcgatcgcg cggcagaaac
     9961 ccagtcaacg ctgacctctt ccggtgcgat ggcggcggtg attggcttcc agtcgccgta
    10021 taacgaccag cgcagcgtga ttgcgctgtt ggcagatagc ccacgcggtt atgaaatgct
    10081 taacgatgcg gtgaacgata gcggcaaacg cgccaccatg ttcggttcgg tcgcggtgat
    10141 ccgcgagtcc ggtatcaaca gcctacgtgt tggcgacgtt tattacgtag gtcatctgcc
    10201 gtggttcgag cgcttgtggt atgcgctggc aaaccatccg attctgctgg cggtgctggc
    10261 gactatcagt gtgatattgc tggcatgggt actgtggcgt ctgctgcgaa ttattagtcg
    10321 tcgtcgtctt aacccggata acgagtaatt gaagatgaat gtgttgcgta gtggaatcgt
    10381 gacgatgctg ttgttggctg cctttagtgt tcaggcagcc tgtacctggc ctgcctggga
    10441 gcagtttaaa aaggattaca tcagtcagga agggcgcgtc atcgacccca gcgacgcgcg
    10501 caaaatcacc acctccgaag ggcaaagtta cggtatgttc tttgccctgg cggctaacga
    10561 ccgtgcagct ttcgataata ttctcgactg gacgcagaac aatctcgctc agggttcttt
    10621 aaaagaacgt ttgcccgcct ggctgtgggg caagaaagag aacagtaagt gggaagtgct
    10681 ggacagcaat tcggcctccg atggtgatgt ctggatggcc tggtcgttgc tggaggcggg
    10741 gcgtttgtgg aaagagcagc gttataccga catcggcagc gcgttgctaa aacgtatcgc
    10801 gcgggaggaa gtggtgacgg tgcctgggct gggttccatg ttgttaccgg gcaaagtggg
    10861 ttttgctgag gataacagct ggcgttttaa ccccagctac atgccgccga cgctggcgca
    10921 gtatttcacc cgctttggcg cgccgtggac cacgctgcgc gaaaccaatc aacatttatt
    10981 gctggaaacc gccccgaaag gcttttcgcc agactgggtg cgctatgaga aagacaaagg
    11041 ctggcagcta aaagccgaaa aaacattgat cagcagctac gacgctatcc gcgtttacat
    11101 gtgggtaggc atgatgcctg acagcgatcc gcaaaaagcg cggatgctca accggtttaa
    11161 accgatggcg acattcactg agaaaaacgg ttatccgccg gaaaaagtgg atgtggctac
    11221 ggggaaagcg cagggtaaag gaccggtcgg tttttctgcc gccatgctgc cttttttaca
    11281 aaaccgcgat gcgcaggcag ttcagcgcca gcgcgtggcc gataactttc ccggcagcga
    11341 tacctattac aactatgtgc tgaccctgtt tggacaaggc tgggatcaac accgtttccg
    11401 cttctcgaca aaaggtgagt tattacctga ctggggccag gaatgcgcaa attcacacta
    11461 aacatattca cgctttccct cggtctggcc gtcatgccga tggtcgaggc agcaccaacc
    11521 atcagcagac cctgcggatg gacagcggca acactaatgc cgtgcgcggg ctggcgaata
    11581 tttaccgcca gcagtcgcca gaaaaagctg aagcgtttat cgcctcgctc tctgccagcc
    11641 agcggcgcag cattgatgat atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg
    11701 cagaggtact ggaaaaccag ggcaaatggg cgcaggcggc agcacttcag cggcaacgac
    11761 tggcgctggg ccccggcagc gtatggatta cttaccgact ggcaggaaga agctgcgcgg
    11821 caatccaacg atggcggcag cagtcagggc ttcggctaca cggcgcgggc attacttgaa
    11881 cgacgtgtta cttccaactg gtttgttggc acggcaattg atatccagca ggtgaaagat
    11941 tacgcaccca gccatttcct gctctacgta cgttattccg ccgccggatg gcagggtgac
    12001 atggatttac cgccgcagcc gctgatacct tacgccgact ggtaagtttt cagatagcgc
    12061 ctctcttaat gccgctgcga tcgggtatac tcgggcggca atctgggatt tccgggggga
    12121 gacaatttgc gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt
    12181 gtcctggtgt tcgtttttat tttttgcacc gttttgctgt tccatctggt ccagcagaat
    12241 cgctataaca cggctacgca actggaaagc attgctcgct ctgtccgcga acccttatct
    12301 tcagctattt tgaaaggcga tattcccgaa gcggaagcta ttcttgccag cattaaaccg
    12361 gcaggcgtgg tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa
    12421 agttttattc cagagcgtcc ggtgccggta atggttactc gcctgtttga gctaccggtt
    12481 caaatctcgc tgggcgtcta ctcgctcgaa cgtccggcaa atccgcagcc aatagcctat
    12541 ctggtgctac aggcggattc cttccgtatg tataagttcg tgatgagcac tctctcaacg
    12601 ttagtgacca tttacttact tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt
    12661 aaccgcctga ttttgcatcc gttacgcaat attgctcgcg aacttaacgc catcccagcc
    12721 caggagcttg ttggtcacca actggcatta ccgcgtctgc atcaggacga tgaaatcggt
    12781 atgttggtgc gcagttacaa cctcaaccag caattgctgc agcgccatta tgaagaacag
    12841 aacgaaaatg cgatgcgctt cccggtgtcg gatttgccga acaaagcctt gctgatggag
    12901 atgctggagc aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa
    12961 accctgcgtg atactgcggg cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg
    13021 ctggtggaaa aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca gattagcggt
    13081 tatgactttg ctgtcattgc caacggtgta caggaaccgt ggcacgcaat cactttgggt
    13141 cagcaagtac tcactatcat gagcgagcgc ctgccgattg aacgtattca actccgtccg
    13201 cactgtagca ttggcgtggc gatgttctac ggcgatctca ccgccgaaca gctttacagt
    13261 cgcgctattt ctgcggcatt taccgctcgc cataaaggca agaatcagat tcagttcttt
    13321 gatccgcagc agatggaagc agcccagaag cggttgacgg aagagagcga tatccttaat
    13381 gcactggaaa atcatcagtt tgcaatttgg ttacagccac aggtcgagat gaccagcggt
    13441 aaactggtca gtgcggaagt gttactgcgt atccagcaac cggatggcag ttgggacctg
    13501 ccggatggct taatcgatcg cattgagtgc tgtgggctga tggttaccgt cggtcactgg
    13561 gtgctggaag agtcctgtcg attgcttgca gcctggcaag agcgcggcat tatgctgccc
    13621 ttgtcggtaa acctctctgc gctgcaactg atgcacccga atatggtggc ggatatgctg
    13681 gaactgttaa cccgctatcg cattcagccg ggaacactga ttctggaagt gacagaaagc
    13741 cgacgtattg acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga
    13801 gttcgggtgg cgctggatga tttcggcatg ggctacgcag ggctgcgtca gctgcagcat
    13861 atgaaatcgt tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg
    13921 gaagatagca gcatgattgc tgcaattatc atgctggcgc agagcctgaa cttacaaatg
    13981 attgccgaag gcgtggagac tgaagcacaa cgagactggc tggcaaaagc gggcgttggt
    14041 attgcccagg gcttcctttt tgctcgccca ctccctattg aaatcttcga agagagttac
    14101 ctggaagaaa agtagctacc ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc
    14161 gagccagctc aaacttttta acctttttgt ttcaattatg atccaggtac atttctgtga
    14221 tgttgtctgg gtgttatttt aaggccgcag gtaccccccc ccataacctt acaagacctg
    14281 tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt actttcaggt
    14341 cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa taggcgagca
    14401 aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg ctcctgtcat
    14461 cttttgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg tcggtcgtac
    14521 cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga ttattggtct
    14581 tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg caacgcttga
    14641 tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg tcgccttcat
    14701 tatggatgtc atcccggcga gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt
    14761 gctgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca aaggccaact
    14821 gatttttaac gtgatcgaaa gtttctcgca ggtcatcttc ggcatcatca atatgatcat
    14881 gcgtctggca cctattggtg cgctcggggc aatggcgttt accatcggta aatacggcgt
    14941 cggcacactg gtgcaactgg ggcagctgat tatctgtttc tacattacct gtatcctgtt
    15001 tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct tcaaatttat
    15061 ccgctacatc cgtgaagaac tgctgattgt actggggact tcatcttccg agtcggcgct
    15121 gccgcgtatg ctcgacaaga tggagaaact cggctgccgt aaatcggtgg tggggctggt
    15181 catcccgaca ggctactcgt ttaaccttga tggcacatcg atatacctga caatggcggc
    15241 ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa tcacgctgtt
    15301 aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg gctttatcgt
    15361 gctggcggcg acgctctctg cggtgggcca tttgccggta gcgggtctgg cgctgatcct
    15421 cggtatcgac cgctttatgt cagaagctcg tgcgctaact aacctggtcg gtaacggcgt
    15481 agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac tggacgatgt
    15541 gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat ctcaccactt
    15601 atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa
    15661 cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt catgttcttg
    15721 cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacggg cagttttttt
    15781 attaccagga tagttgatca ggggttcaca tgcagggcat aaaaattcga cttttagcgg
    15841 gcggtttgct gatgatggcc actgctggct atgtgcaggc agatgcgctc cagcctgatc
    15901 cagcatggca acaggggacg ctttccaacg gtttacagtg gcaagtgctg accacccccc
    15961 agcgtcccag cgatcgtgtt gaaattcgcc tgctggttaa taccggttcg ctcgccgaaa
    16021 gtacacaaca gagcggttac agtcacgcca tccctcgtat tgcgctaacg caaagcggtg
    16081 gccttgacgc agcacaggcg cgttcattgt ggcagcaggg gatcgaccct aaacgcccga
    16141 tgccgccggt aattgtctct tatgacacca cgctgtttaa tctgagtttg cccaataacc
    16201 gtaacgattt gctgaaagaa gcgctctctt atctggcaaa tgccactggc aaattgacca
    16261 tcacaccaga aaccatcaac cacgcgctgc aaagtcagga catggtggca acctggcctg
    16321 ccgatactaa agagggctgg tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg
    16381 atcctgccga tccgctgaaa caacccgttg aagcggaaaa aattaaagat ttctatcaga
    16441 aatggtacac cccggatgca atgacgctac tggtggtggg aaacgtggat gcgcgctcgg
    16501 ttgtcgacca aatcaacaaa acgtttggcg aactgaaagg caaacgtgaa acgccagctc
    16561 cggtgccgac gctttctccg ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc
    16621 gtcaggaccg gttatctatc atgtgggata cgccgtggca gccgattcgt gaatcagccg
    16681 cactgctgcg ctactggcgt gcggacctgg cccgcgaggc gctgttctgg catgttcagc
    16741 aagcgttaag cgccagtgac agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt
    16801 atctgcgtgc gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg aacagcaacc
    16861 ttaatctggt ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt
    16921 tcaatgcgtt agtggcgcaa aagaaactgg agctgcagaa actgtttgcc gcctatgcac
    16981 gggctgatac cgatattctg atgggccagc ggatgcgttc gttgcaaaat caggtcgtcg
    17041 atatcgcgcc ggagcagtat cagaaactgc ggcaggattt ccttaacagc ctgactgtgg
    17101 agatgttaaa tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta atactgctgc
    17161 agccgaaagg cgagccggaa tttaacatga aagcgttgca ggcggcctgg gatcaaatca
    17221 tggccccatc taccgccgct gcgaccacct ctgtcgccac ggatgacgta catcctgaag
    17281 tgacggatat tccacctgca cagtaatgcc tgatggtgat gagttagcac aaccatcagg
    17341 tatttatgga tgagctggat agaggggtaa cactttatcc agccttttgc atatgctgcg
    17401 tttacgctgg catcgcctca cgcgggataa tcgcgccgcg atactgaata acggtacttg
    17461 cggtcaggtg cccgcgtttc gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca
    17521 gataaccggc gctgaaagag tcgccagctg cggtggtatc gatcactttt tctttcggca
    17581 gtttcaccgc cggaacatca actaacgctt caccagcaat ggacaccagg caagaatccg
    17641 ccccgcgttt caccaccact tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt
    17701 ccaccggctg ttgaccccac agcgcgtctt cgtcgtccag cgtcaggaag gcgatatccg
    17761 tgcattccag catttgctgg tacacctgct gtgtctcttc tttgctggcc cacaggcgcg
    17821 gacgatagtt attgtcgaaa atcacttttc cgcctttggc gcggcattcg cgcagcaggg
    17881 aaagcagctt ttcgcggctg gtcgggctta agatcgccag gctaatcccg ctcaggtaga
    17941 gataatcgaa attcgccagc tcttcgcaaa tcgccgcaga ctgctcgctc gccagccaga
    18001 atttggcggc ggcttcgtta cgccagtagt agaacgtacg ttcgccggtg ctgtcggttt
    18061 caatgtagta aaggcccgga agacggtttt ccatccgttg ggtcagggaa gtatcaacgt
    18121 tctcgccgtg ccaggcgtcc agcatctgct ggctaaaact gtccgttccc agcgccgtta
    18181 cgtaatgaac ggttaatgcc gcaggatcga cctgacgggc gatatagacg gaagtgttca
    18241 gggtatcgcc gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc
    18301 attcgccaat cacggcaatc tttttggaca tagtcgtgaa cctgatctgt aaaaaattag
    18361 cgttagtttg cgctgtggtg gtttgctggt caaccatatt aaaacagtgt tccattattt
    18421 ttttgagcca gaacgtattc ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc
    18481 caggcggtaa agttctgccc ttacgcgccg ataatctttg tcgagtccgg gcagcatcac
    18541 ttttaaacac aggacatctt tgatgataag gcaggttatc cagcgaataa gcaaccctga
    18601 agcaagcatc gagagcttgc aggaacggcg tttttggttg cagtgtgagc gtgcttacac
    18661 ctggcagccg atctatcaaa catgcgggcg gttaatggcc gtggagctat taacggtggt
    18721 cacgcatccc ttgaaccctt cgcaacgcct gccgccggat cgctatttta ctgaaatcac
    18781 cgtcagccat cggatggagg ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga
    18841 cttctttgta gagcacggcc tgctggcatc ggtcaatatt gatggcccta cgctcatcgc
    18901 cctgcgtcag caaccaaaaa tcctgcgcca gattgagcgt cttccctggc tgcgtttcga
    18961 actggtggag catatccgtc tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg
    19021 cccgctgtgg ctggatgatt ttggtaccgg gatggcaaat ttctctgcac taagtgaagt
    19081 gcgttatgac tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga
    19141 aggccgcaca ctcttttctc agcttttaca tctaatgaat cgctattgtc gcggggtgat
    19201 tgtcgagggc gtagaaacac cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc
    19261 cgcacaaggc tggtttcttt cacgcccggc accgatggaa acgctgaata cggcggttct
    19321 ggcgctataa gctgcctcat tttccgccgg gctggactat ctttaggact ggcacaggaa
    19381 gaatatgagg aagcgaacga tgagcaaggc aggcaaaata accgctgcga tttcaggggc
    19441 tttcttgttg ttgattgtcg tggcgatcat tttgattgca acttttgact ggaatcgact
    19501 caaaccgacc atcaaccaga aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg
    19561 caatctgggc gtggtgtggg agcggcaaaa acaagaaact ggctggcgca gctgggtgcc
    19621 gtggcctcat gtacatgcag aagacatcat tcttggtaat ccaccggata ttcccgaagt
    19681 cacgatggtg catttgccgc gagtagaggc aacgctggca ccgctggcgc tgctgaccaa
    19741 aacggtctgg ctgccgtgga tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc
    19801 tgaaaagaac aataactgga cgtttaatct tgccaacgat gacaacaaag acgcgaatgc
    19861 aaagccgtcg gcatggtcgt ttcggctgga taatattctt ttcgatcaag ggcggatcgc
    19921 cattgatgac aaagtaagca aagcggatct ggaaattttt gttgatccgt taggcaagcc
    19981 gctgccattc agcgaagtta ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga
    20041 ttacattttt ggcctgaagg cgcagggacg ttataacggt gaaccgctca cgggtacggg
    20101 aaaaataggc ggtatgctgg cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga
    20161 tttccgttca ggtaataccc gtgttgcttt tgatggcgtc gtgaatgacc caatgaagat
    20221 gggcggtgtc gatttacggc ttaaattttc tggcgattca ctgggtgatc tctatgaact
    20281 gacgggcgtt ctgctgcccg ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa
    20341 aatcgacact gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc gaattggcga
    20401 tagcgatatc cacggttctc tggtctacac caccggaaag ccacgaccaa aactggaagg
    20461 tgatgtcgag tcgcggcaat tgcggctggc ggacctggga ccgttgattg gcgttgattc
    20521 cgggaaaggg gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca
    20581 gcctgcgggc aaagtgctgc cttatgaccg cttcgaaacc gataaatggg acgttatgga
    20641 tgccgatgtt cgcttcaaag ggcggcgcat tgagcatggc agtagcctgc cgattagcga
    20701 tctttctact catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg
    20761 catggcgggc ggcagcattg cggcgaatat tcatctggaa ggcgataaaa agccgatgca
    20821 ggggcgggca gatattcagg ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga
    20881 actgatgcag aagacgctgg gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa
    20941 ctcggtggcg gcgcttttag gcaacagtaa cggcaacctg aaactgttga tgaatgacgg
    21001 gctggtgagc cgcaacctga tggagattgt tgggctgaat gtcggcaact acattgtcgg
    21061 tgcgatattt ggtgacgatg aggtgcgggt gaactgcgcg gcggcgaatc tgaatattgc
    21121 caacggcgtg gcgcgcccgc agatttttgc tttcgatact gagaacgcgt tgattaatgt
    21181 taccggcacg gcaagttttg cttcggaaca gctggatttg actattgatc cggagagtaa
    21241 agggattcgg attatcacac tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc
    21301 gcaggctggg gtgaaagccg gaccgctgat tgcccgtggt gctgttgctg cggcactggc
    21361 aacgctggta acaccggcgg cggcgttact ggcactgatc tcaccttccg aaggggaggc
    21421 taatcagtgt cggacgatat tgtcgcagat gaagaagtga tgataattgc cggatgtctg
    21481 acatccggca attaaacaga tttagatttt tggcaaagtg tgctttgttc atgccggatg
    21541 cggcgtgaac gccttatccg gcctacaaat ccttgtgaat tcaatatatt gtatggaaat
    21601 tcagtcctga taagcgtagc gcatcaggct tttcacactt attacaacga ctgatgtcgc
    21661 gtctcatggg tcagcagcag ggcgattaac gtcaagccag ccatcgccgc cagatataac
    21721 cccaccgcac ctaacccgta gttagcctgc aaccaggctg cgatatacgg cgcaacggaa
    21781 gccccgagaa tcgacgatac gttgtaagag aacgatgctc cggtgtaacg cacttctgtc
    21841 ggaaacagct ctggtaacag cgcacccatc ggcccgaagg tcagccccat cagacttaac
    21901 cccagcagca ggaaggcaaa aaccagaatc gggttgccag aaccgagcag cgggttaaag
    21961 gcgaacagcg cgaacaggat gatcagcgtg gtgatgatca ccatgctttt acggcgacca
    22021 aaggcatcag ccagtaatcc ggcgactggc accatcacgc caaaaccaat aactgccatc
    22081 atcagcatcc acaacacttc gttacgcggc aggccaagcc caactggcgc ggcggcggta
    22141 ctgaaggtca tagagtagac cgtcatgatg taaaacagcg tataggttgc cagcataatg
    22201 aacgtaccca gtacggttac gcgaacatgt ttggtcagca gcgtacccag cgggatcttc
    22261 acctgttttt tcgctttagc gactttctca aacaccggcg actcatgcag cgacacgcga
    22321 acatacaggc cgataatgac cagcaccgcc gagaagataa atggcacacg ccagccccag
    22381 ctcataaact gctcatcggt cagtagccag gaaagcagca agaaagtgcc gttggcaaag
    22441 aagaagccaa tcggtgcgcc tagctgtggg aaggagccat acagtgcacg tttgcgcggt
    22501 ggggcgtttt cagtcgccag cagcgccgcg ccgccccatt caccgcctaa gcccagaccc
    22561 tgaccaaatc gagccagcgc cagcagcagt ggggcgaaaa taccaatcgt ggcatagccc
    22621 ggcagcagac caatcaccac ggtcgaaatc cccatcgtta gcaacgaggc gaccagcgtc
    22681 gctttacgcc caacgcgatc gccaaaatga ccaaaaacgg cagagccaat ggggcgcgcg
    22741 acgaaggcga tggcgaaggt ggcgagcgac tgtagcgttg ctgccgttgg atcgccctgc
    22801 gggaagaaga tatgcggaaa cacaataacg gccgcagtgg cgtaaatgta aaagtcgaag
    22861 aactcaatgg ccgtgccaat gagagaggcg acaaggactt tattacgcga gttgatcggc
    22921 gtgtattctt gttcgtggtc gagtgttgtg gctgttgctt gcataatctt ttcttatttt
    22981 ttggctaacg aatagccata ttacgcacag caaaagtgat atttcaatgt gtaacaaatc
    23041 gcggaaatgg tcaaaaacca ggcaaaaagg ggataatgtt taggccagtg atttttcttc
    23101 tatgaaatgc ttcacataat tttataaata gtggataaaa ctggtttcag gttaaataaa
    23161 agttaccaac tggatttata tgctggggtt atgagttgga gtgaaattgt ttgctcccaa
    23221 atagatggct gaagaggctc tggggaagag tttcaagagg ttatgtgcgc attatagccg
    23281 gatgcgatgc tggcgcatct tatccggcct acggggagcc aggccgaata aaatcgtatg
    23341 ccggataagg cgtttacgcc gcatccggcc tattttaagg ctgcgttttc cctggcattc
    23401 gcgggtcgtc tttatattcg gcggtggcaa tccacgccgc gcaaaacagc gtcaaacggg
    23461 cgaagaagta gaaaaatgcc atcagcccca gcacggaacc aaatgctgcg ccagaggggg
    23521 atttcatcag cgatggcagg gtgtaggtca tcacgatttt aatcacttca aaaccaatag
    23581 cagccagaaa tgttccgcga atcagggctt ttttgcgcgg acggtggcgc ggcaaccgcc
    23641 agaagatcca gaagaaaagc agatagttgg cgaagatgga aatcgccagg ccaatcaatc
    23701 gccacgtcgg tttcagccac tcaatgctgt tcaggtgcag ggcgctaata atcatttgct
    23761 gcgccgaacc ggcaaccgag gtgatcgaaa gcgtcacaat cagcgcaatc aacaaaccaa
    23821 tcagtgaaat aaaatcacgc agatatttta cccagaactt ctcctgatct tgcggcgagc
    23881 gttcccagac atcgcgcgac tgggcacgaa tcgcttcacg caggttaccc atccagttga
    23941 tgccggaata aagtgccacc gccaggccga caagccctac agtcgtacgc tgctgaacgg
    24001 cggtgttgat ggtgtttttc aacgtggcgg ctagtgtcgg atcgctgatg ttttgcagaa
    24061 ttttgtcgaa gatatcctgt agcaacatcg gatgggaggc cagcacaaag ccccctgcgg
    24121 caaacgacac catcaaaatc gggatcatcg acaaaaatga gaaataggtg atagccgcac
    24181 caaactggtt acccagccga tcgttaaagc gttctgtcgc gcgaataagg tgcgcaataa
    24241 ccggttggcg ttggactttt tcggcagtgg tggtgacggt ttccagcgcc tggctaactt
    24301 tgccgccttt ctcgctatta tccaactgct taatcggctc gtgctccaga tcctgggtgg
    24361 gacgtttgat ctcgttttcc tgcgtcatcc gctcttttgt ccttctgtcg tggtcattct
    24421 taaaaagtat agtcggtcag tccaggtgct ctttcattac gccagccagc cactgcataa
    24481 acaggtttac acgccgggaa agctcccgac gctgcgggta aaccagcgaa agggagagcg
    24541 gctcggcacg gtagcctggc aacacttcaa taagccgccc ggcacgcagg gcttcgcgca
    24601 cggcaatgcg cgggatctga ataatcccca gaccggcaag accggcggcg agatagtttt
    24661 cgctactgtt taccgtcaac atgccgccag acttaaacca ctggacgcca ttaacgctgg
    24721 caacctcaaa acctaacgga tgtacaccca ggtgcggtgt ataacgcact attgcgtgtg
    24781 aagtcagatc atcggggctt tgcggataac caaagcgcgt cagatagtgc ggactggcac
    24841 agttgaccat ggttagtttg ccgaggggac gggcgataac gccatcttcc ggtaacgcgc
    24901 cagtgcgtat cacgcaatca aaaccatcat gaagaatatc taccggacgg ttatggctac
    24961 tcagttccag ctcaattccc ggatgctgat agagaaattc cgacagacgc ggcagtaaca
    25021 ggtttttcgc gattcctgac gggatgtcga tgcgtaattt accgctgata ctggtggcat
    25081 cctgttgaaa cagaccgtcc agttcgctga gattactcaa cacatctttt gcccgttgat
    25141 aataggtcat gccttctggc gtgagtttga cccgtcgcgt agtgcgctgg agaagctggg
    25201 tgccaagttg atgttccagt gcctgtatct ggcgcgaaac acttcccttt ggcaaagcaa
    25261 agaaatccgc cgcgcgggaa aaactttcca gctccgcgac tttgatgaac aactgcattg
    25321 cgtgaatttt atccatttct ttgaccgatt gttgttcaca gcgaaacagt aacgcgcata
    25381 tcgcattgtt tattgttttt tctaacatct aatgccgggt ttcacaatgt tgtcatgacg
    25441 aatgtggcga tttctcagca ggaaaagggc ggaatagctt tatgagaaag gtgaatcccc
    25501 ttaaacagag cgtatttttc agtgattccc tttaaagtga atagaggacg tattggctat
    25561 ttgtgcttat ttttgtctca ttgccatttg ttattttggc ttgtagtcgc ggaatttcat
    25621 ttttaattgc atgcgtacta accaccaata taaggaaatc ctcaaaattg ttttaaatag
    25681 tgccctcgac atgtaatttc aggcgtttag ctgtaactat ttatatcggt aactattttc
    25741 tacgcgtcat ctctgtagaa tggccaccgc caaatggcga attcttattt tttctcgcgg
    25801 gtaataccct tccactactt aagtcttttt aacgtattca ggtggagatt cagttatgca
    25861 aatagtcatg tttgacaggc agtcaatatt tattcatgga ataaaaatca gtttacagca
    25921 gcgtattcca ggagtgagta ttcagggggt cagtcaggca gacgagttat ggcaaaagct
    25981 ggaaagttac cctgaagcct tagttatgct cgatggcgat caggatggtg agttttgcta
    26041 ctggttgctg caaaaaaccg tggtgcagtt tcctgaggtt aaggtgttaa tcacggcgac
    26101 ggattgcaat aaacggtggt tacaggaagt tatccatttt aatgtgctgg ccattgtgcc
    26161 tcgcgattca accgtcgaga catttgcgtt ggcggtaaat agtgcggcga tggggatgat
    26221 gtttctgcct ggagactggc ggacgacgcc ggaaaaggac attaaagatc tcaaatcgtt
    26281 aagcgcccgc cagcgggaga ttttaaccat gttagcggcg ggcgaatcaa ataaggagat
    26341 cggcagagcg ctgaatatca gtaccggaac ggtaaaagcg catctggaat ccctgtatcg
    26401 ccgtctggaa gtgaaaaatc gcacccaggc ggcaatgatg ttaaatatct cctcctg
//
LOCUS       DAJTMX010000049        25771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTMX010000049 DAJTMX010000000
VERSION     DAJTMX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.049"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..441)
                     /locus_tag="OO145_002015"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO145_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO145_002016"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO145_002016"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5467138.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO145_002017"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO145_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5467139.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO145_002018"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO145_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5467140.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO145_002019"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO145_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO145_002020"
     CDS             5754..6257
                     /locus_tag="OO145_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5467141.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO145_002021"
     CDS             complement(6337..6588)
                     /locus_tag="OO145_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5467142.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO145_002022"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO145_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5467143.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO145_002023"
     CDS             7052..7375
                     /locus_tag="OO145_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5467144.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO145_002024"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO145_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5467145.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO145_002025"
     CDS             complement(8080..8319)
                     /locus_tag="OO145_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5467146.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO145_002026"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO145_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5467147.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO145_002027"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO145_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5467148.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO145_002028"
     tRNA            complement(10643..10729)
                     /locus_tag="OO145_002028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO145_002029"
     tRNA            complement(10742..10815)
                     /locus_tag="OO145_002029"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO145_002030"
     tRNA            complement(10870..10945)
                     /locus_tag="OO145_002030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO145_002031"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO145_002031"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5467149.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO145_002032"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO145_002032"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5467150.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO145_002033"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO145_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5467151.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO145_002034"
     CDS             14483..14659
                     /locus_tag="OO145_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467152.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO145_002035"
     CDS             14646..14870
                     /locus_tag="OO145_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5467153.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO145_002036"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO145_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO145_002037"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO145_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5467154.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO145_002038"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO145_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5467155.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO145_002039"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO145_002039"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5467156.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO145_002040"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO145_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5467157.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO145_002041"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO145_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5467158.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO145_002042"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO145_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO145_002043"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO145_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5467159.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO145_002044"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO145_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5467160.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO145_002045"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO145_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5467161.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO145_002046"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO145_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5467162.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..>25771
                     /gene="amyA"
                     /locus_tag="OO145_002047"
     CDS             24847..>25771
                     /gene="amyA"
                     /locus_tag="OO145_002047"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5467163.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDM"
BASE COUNT         6535 a         6635 c         6032 g         6569 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgctcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg a
//
LOCUS       DAJTMX010000050        25662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTMX010000050 DAJTMX010000000
VERSION     DAJTMX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.050"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO145_002048"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO145_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5467164.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="OO145_002049"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="OO145_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5467165.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="OO145_002050"
     CDS             2630..3391
                     /locus_tag="OO145_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5467166.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="OO145_002051"
     CDS             complement(3442..4326)
                     /locus_tag="OO145_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5467167.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO145_002052"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO145_002052"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5467168.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="OO145_002053"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="OO145_002053"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5467169.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="OO145_002054"
     CDS             6202..6474
                     /locus_tag="OO145_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5467170.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="OO145_002055"
     CDS             6844..7920
                     /locus_tag="OO145_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5467171.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="OO145_002056"
     CDS             7947..9326
                     /locus_tag="OO145_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467172.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="OO145_002057"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="OO145_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="OO145_002058"
     CDS             10409..11245
                     /locus_tag="OO145_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5467173.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="OO145_002059"
     CDS             11250..12197
                     /locus_tag="OO145_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5467174.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGDDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="OO145_002060"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="OO145_002060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5467175.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="OO145_002061"
     CDS             13324..14082
                     /locus_tag="OO145_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5467176.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="OO145_002062"
     CDS             14200..15558
                     /locus_tag="OO145_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467177.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO145_002063"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO145_002063"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5467178.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO145_002064"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO145_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5467179.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO145_002065"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO145_002065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5467180.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="OO145_002066"
     CDS             19503..20054
                     /locus_tag="OO145_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5467181.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="OO145_002067"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="OO145_002067"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5467182.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="OO145_002068"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="OO145_002068"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5467183.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="OO145_002069"
     CDS             23307..24347
                     /locus_tag="OO145_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5467184.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25662)
                     /gene="gdhA"
                     /locus_tag="OO145_002070"
     CDS             complement(24464..>25662)
                     /gene="gdhA"
                     /locus_tag="OO145_002070"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5467185.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGT
                     VVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQN
                     ELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLE
                     MAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAM
                     LAQGVI"
BASE COUNT         6517 a         5971 c         6625 g         6549 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg gggacgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag actctggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tg
//
LOCUS       DAJTMX010000051        25488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTMX010000051 DAJTMX010000000
VERSION     DAJTMX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.051"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..685)
                     /locus_tag="OO145_002071"
     CDS             complement(<1..685)
                     /locus_tag="OO145_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5467186.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRE"
     gene            complement(825..1781)
                     /gene="ytfQ"
                     /locus_tag="OO145_002072"
                     /pseudo
     CDS             complement(825..1781)
                     /gene="ytfQ"
                     /locus_tag="OO145_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            2091..2621
                     /gene="ppa"
                     /locus_tag="OO145_002073"
     CDS             2091..2621
                     /gene="ppa"
                     /locus_tag="OO145_002073"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5467187.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(3001..3342)
                     /locus_tag="OO145_002074"
     CDS             complement(3001..3342)
                     /locus_tag="OO145_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5467188.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(3345..7124)
                     /gene="tamB"
                     /locus_tag="OO145_002075"
     CDS             complement(3345..7124)
                     /gene="tamB"
                     /locus_tag="OO145_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5467189.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(7121..8854)
                     /gene="tamA"
                     /locus_tag="OO145_002076"
     CDS             complement(7121..8854)
                     /gene="tamA"
                     /locus_tag="OO145_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5467190.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            9060..9698
                     /gene="msrA"
                     /locus_tag="OO145_002077"
     CDS             9060..9698
                     /gene="msrA"
                     /locus_tag="OO145_002077"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5467191.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            10021..11364
                     /locus_tag="OO145_002078"
     CDS             10021..11364
                     /locus_tag="OO145_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5467192.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(11426..11632)
                     /locus_tag="OO145_002079"
     CDS             complement(11426..11632)
                     /locus_tag="OO145_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5467193.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            11957..12511
                     /locus_tag="OO145_002080"
     CDS             11957..12511
                     /locus_tag="OO145_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5467194.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(12574..13512)
                     /locus_tag="OO145_002081"
     CDS             complement(12574..13512)
                     /locus_tag="OO145_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467195.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(13724..14464)
                     /gene="cysQ"
                     /locus_tag="OO145_002082"
     CDS             complement(13724..14464)
                     /gene="cysQ"
                     /locus_tag="OO145_002082"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5467196.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            14654..16597
                     /gene="cpdB"
                     /locus_tag="OO145_002083"
     CDS             14654..16597
                     /gene="cpdB"
                     /locus_tag="OO145_002083"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5467197.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(16723..17103)
                     /locus_tag="OO145_002084"
     CDS             complement(16723..17103)
                     /locus_tag="OO145_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5467198.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            17192..18052
                     /gene="qorB"
                     /locus_tag="OO145_002085"
     CDS             17192..18052
                     /gene="qorB"
                     /locus_tag="OO145_002085"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5467199.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            18161..19126
                     /locus_tag="OO145_002086"
     CDS             18161..19126
                     /locus_tag="OO145_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5467200.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            19234..19896
                     /gene="ytfE"
                     /locus_tag="OO145_002087"
     CDS             19234..19896
                     /gene="ytfE"
                     /locus_tag="OO145_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5467201.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(19941..21353)
                     /gene="cycA"
                     /locus_tag="OO145_002088"
                     /gene_synonym="dagA"
     CDS             complement(19941..21353)
                     /gene="cycA"
                     /locus_tag="OO145_002088"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5467202.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(21661..22281)
                     /gene="fklB"
                     /locus_tag="OO145_002089"
     CDS             complement(21661..22281)
                     /gene="fklB"
                     /locus_tag="OO145_002089"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5467203.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            22500..23045
                     /locus_tag="OO145_002090"
     CDS             22500..23045
                     /locus_tag="OO145_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5467204.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(23029..23676)
                     /locus_tag="OO145_002091"
     CDS             complement(23029..23676)
                     /locus_tag="OO145_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5467205.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            23888..24679
                     /locus_tag="OO145_002092"
     CDS             23888..24679
                     /locus_tag="OO145_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5467206.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            24689..>25488
                     /locus_tag="OO145_002093"
     CDS             24689..>25488
                     /locus_tag="OO145_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5467207.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6243 a         6691 c         6238 g         6316 t
ORIGIN      
        1 tttcccgaca gcctacgaaa ctgccgttgc gtaagacggt gatccgatcg ctgacctgat
       61 agacctgatc gagaaagtga gtgacaaaga tcaggctgac gccgcgatcg cgcaactgac
      121 gcatcaggcc aaataatagc tccatttcct gggtgtcgag gctggcggtg ggttcatcga
      181 ggatcagcac tttggcggag agatcgatag cccggcaaat ggcgacgatt tgctgcatcg
      241 ccaccgaaaa acggttaagc ggttcgcgca cgtcgaggga gaaaccgtaa gatgccatca
      301 gttcggtggc gcgcttttcc atctctttac ggcgtagaag gccgaaacgt ttgggttcgc
      361 gacctatgaa tagattatca gcaaccgaca tattgggtag caggttgact tcctgataga
      421 cggtgccgat accgagttgt tgtgcatggg cggtattttt cggtgagata gtctggcctt
      481 ccagccagat ggtgccgcga tcggcgtggt atacaccagt taatgctttg attagcgttg
      541 attttcccgc cccgttttca ccaagcagcg ccatgatttc gccacggcgc aggctgaaat
      601 caacgttgtc taacgctttg acgccgggga aaaatttact taatccttcg gtgcggagga
      661 tctcctggtg ttggtcggtc gtcatggttt cccccggtgt aaagcgtgct cccagtaggc
      721 atgataagac gcggtaagcg tcgcatcagg cttcagtgca ccatcgccgg atgcggcgcg
      781 agcgccttat ccggcctacg aatcggcatc aggcatagca accctcaata ccccatattt
      841 ttcttcttct ctaactcttc ttttgcggta tcaggcaggt aaagggtgga tttggttaac
      901 gtcagctttt caggcatggt gccgtctttt ttgtatttct ccagcgcatc gaaagcgggt
      961 cccgccatgt tcggcgtcag ttcaacactg gcgttcgctt cgccatccat catcgctttg
     1021 tagatgtccg gtacaccgtc aatggaaccc gtgaggatat ctttgcccgg tttcaggccc
     1081 gcttctttaa ttgcctgaat tgcaccaatc accatgtcgt cgttatgggc gtaaaccatg
     1141 cagatgtttt tgccgttgtt ttccgctttg ataaagctct ccatgacttc tttgccttta
     1201 ctgcgggtga agtcacctga ctgcgagcgg ataattttga tatttggcgc attcttaatg
     1261 gcttcggcaa agcctttctt acggtcaatg gcgacgctgg ccccaacggt gccctgcagc
     1321 tccaccacgt tgcatggttt gccattcact tcttttacca gccagtcacc gatcaactta
     1381 ccttcgagga tgttgtcggc agtaacggtg gtcatataga gagatttgtc tttcacatcg
     1441 atggaacggt cgagcaagaa gaccgggatt tcggcatctt tcgcctcttt taataccggt
     1501 tcccaacccg tcgcaaccac tggagcgata aagatcgcat ccacccctta tgcaacgaag
     1561 gaacgtaccg ctttaatctg gttttcctgc ttttgctgac catcggcaat tttcaacgta
     1621 attccgcgtt tttcggcctc acttttcgcc acattggttt ctgcggcgcg ccagccggat
     1681 tccgatccga cctgcgaaaa tcctacggtt aatggagcgg ccaacgccat agacgacatg
     1741 gctgccgaga ctgcagagac tacaagtaag cgtttccaca tagggttgtc ctcgtcgggg
     1801 taattattat tggtgaaaag atgttcgcga aaaaactata gacaattcgt tatgtaacgg
     1861 attgcgttac atcacacttc aaaaaagtaa ataaaacgtt aatcacaagt ttgtaatcgc
     1921 tttcatctca ctatgaaaaa tgcggctacg gttatggatt ttcctgctct gtataccgtc
     1981 ttaaaactgg cgaaaaagga aaatgaagac gaaaacaagc aaagacattc ggcgcgagtt
     2041 ggctataata cttggcactt gtttgccaca tatttttaaa ggaaacagac atgagcttac
     2101 tcaacgtccc tgcgggtaaa gatctgccgg aagacatcta cgttgttatt gagatcccgg
     2161 ctaacgcaga tccgatcaaa tacgaaatcg acaaagagag cggcgcactg ttcgttgacc
     2221 gcttcatgtc caccgcgatg ttctatccgt gcaactacgg ttacatcaac cacaccctgt
     2281 ctctggacgg tgacccggtt gacgtactgg tcccgactcc gtacccgctg cagcctggtt
     2341 ctgtgatccg ttgccgtccg gttgccgttc tgaaaatgac cgacgaagcc ggtgaagatg
     2401 cgaaactggt tgcggttccg cacagcaagc tgagcaaaga atacgatcac attaaagacg
     2461 taaacgatct gcctgaactg ctgaaagcgc aaatcgctca cttcttcgag cactacaaag
     2521 acctcgaaaa aggcaagtgg gtgaaagttg aaggttggga aaacgcagaa gccgctaaag
     2581 ctgaaatcgt tgcttccttc gagcgcgcaa agaataaata agttcttcta gcgcaataac
     2641 cctgaacgcc gggcttcggt tagtaagggt ttttttatgc ccgcgataaa taaactctct
     2701 attccaccat cattattctc agcggttgca aggcttgaac ggtaagaaca agcaaacccg
     2761 accaccattt tgctgttcat agccacttgc tggaagttag ccgacctcac tcatactcac
     2821 cgttcaaagc gtgcccccgt tttacccctg cacatttgtg aacgccagca gagtttttta
     2881 tgcgattgca gatatggaaa gcggctcgga gagaaaagca aaagatggaa aagttacaaa
     2941 aaaaaatcgc gagatacatc gcataaaaca acgccacccg aaggaggcgt gcttttatta
     3001 ttacttatcc ctgtctaacc agtcgccgct ttcaattagc tttaatccat cgacgggtcg
     3061 ttgataaacg tacatccatg cactcccata cggcgtctga atcaactggc gcgcgtattc
     3121 accgcccctg gtgcgcaagg catcaagttc ggccagcgtg gcgttgtcaa tacgataaac
     3181 ttcaccgtgt accgttccgt tccccggaac tgcgcctgga tagtggccca ggctatacaa
     3241 ctggtagtta tcgatactga aatcgcccag taactgggca ttggtcatcc agtgactgtt
     3301 gccttgtttg tggcgtaaac tgccgtagac aaatattcgc attgctaaaa ctcgaactga
     3361 tagagcaaat ccagtgcctg gtctacacca gacacggctt ccagatatag cttaggcatc
     3421 aggcgataac gtaacgtgag tgttgctata gagtcaaata tacccacgcc gtatttcact
     3481 tgcagacctg gcaatacata gccgctgacc actacctggg aggagtcgcc tactcccagg
     3541 gtgtcgagcg ctaaattgct tacgccaaac gtctcgccga ttttacccac aatctggcca
     3601 ctttgcgcaa cccccagacc aatcagcatc gaggtcattg ccgcactgtc gctctgatcg
     3661 ctctccagcc cttgtccacg tagcaaataa gacaatgcag cttgttgcga catcgccggg
     3721 tcagagaaga tctccgcttt cggttcgtcc gccagaccag tgacgcgaac tccggcgatt
     3781 acatcgtctt ctgtagcatc cgggttacga atagcttcaa tattaagata cggttgatcc
     3841 ggcggaccag agaacagtaa ctcaccctta cgcacaatca gatcctgacc ataggcatgg
     3901 aagcgccctt cagggatgtt gatctgcccg ttcaggccca gcccttgttt gtcctgtacg
     3961 acgttaagat cgcccgtcag ccgcgctttc aggccaaagg cgtcaatgcg cacattgttg
     4021 ccaacgtgga caatcaggtt actgttaatc ggaatcgacg ccgttttcgg ctcttccggt
     4081 tgcaggttat cgttaagcat caccacatcg ctggagacgc ctactgcgct ttccggcaga
     4141 tcgtgcacca cgatgcgcgc ccacgggaca tccacgcgac catcgagggt aaacaggttt
     4201 ggtgtagcct cgaatacaac atctggcgat acatccattc gtaccatcgg cggcacggtg
     4261 atccgcactt tactgccttt cgccgttact cgtgcccgcc agttttcaat ttggctccag
     4321 tcggcatcac cgttcaggta gatttcaccc tgctgggtcc gtactgtacc ggcaagcgtc
     4381 gagcgcatac cgttaaagtt gaccgcaagc tggctcggct gcatatcaaa cggcataaag
     4441 ttgccgtcga tatccacacc cgtaacctga agctgaccaa acaactgcgg gctttgcaca
     4501 tcaccaccca gacgcaagtt ggcactcacc atccccgctg ctttttcccc acgggtaaag
     4561 atggggttta tcatcgcaag gttgaagtta cggatattga cgttgccacc aagattacgg
     4621 cggccttgcg gatcggtcac ctgcacctgt ccatcaaact ggccgttatt ggtcaggcgg
     4681 atggtccagc ccaattcggc acggttgtta cgcaattccg ccgtcagatt cagtgtctga
     4741 aacgccaccg gcagcgccgc atcgttgacg gtttgcgtta cctgcacgtt acgccccgaa
     4801 agggtgatac tgccctgcgg cagcccctct ttcgtggtgt cccaggcaac atccgcttta
     4861 cccgtgaaga taccgctggc ctgagtggtt tctggcataa acggtttcag catggcgagg
     4921 tcgaagcggt tgagattcac caccgcacgc ccttcggccc ccgcatcgat agtttgcggc
     4981 acgcacagtt ccgcattcgg gttaagccag cagtgtggcc cgatgctgat tttttgctcc
     5041 ttattgcggt aatccagcgc aatatcgcgg gtcagcgacc acgggccgac cggcgtctgg
     5101 aagcgggtat tgctaagagt tcccttccag cgttcttctt tgcgatcaaa acttcctgcc
     5161 agattaagct gcccggagac aggctcgccc tgaatccgca actgtagctc gtgctgcttt
     5221 tcgctgcctt tggcattcag ggtgacgagg ttgatattta catccggctg cgaaatttgc
     5281 tcaacgcgta cgtcgagttt cccggcgatc tgatcggtgg atttaatgtc gccttccacg
     5341 cgaacctgcg ccacggaaag ttcctgccag cgcaggccgc gcgcggtgat atctgccagt
     5401 agttgtggcg cttccaccgt gccgcgtact ttcaccagcc ctttcgctgt accgccaagc
     5461 cccggcagcg cgttatccag ccccggcgcg ttgatggtgg catcaagatt gagatctttt
     5521 acccccagct cgcctttcac ttcggcactg tttggcccga gttccagatg aagcccaggg
     5581 atcatccact gcatataact gttgcctttc agcgtgccgt caacgttcac tttgttctgt
     5641 ttgacgttac cggtcagctt cagttctggc acctccatct gccaggtgcc gccgtacagg
     5701 ctaccgcggg ttttaatcaa gccattgagt ttcgacggcc actccgggaa ctctttggcg
     5761 gtgttaatgc cattaagcgt tagctcaccg cgccaactaa tggcctgctg ccagtcgagc
     5821 aacgccttga gttcagtttt cccttccagc gccgcgacgg tgagtttgtc gagattgacc
     5881 tgctgttcat tacctttggc atcaagggta atggtggctg gcgggatctc ctgtcccttc
     5941 actgccgtac gcatagagag cgtgtaatcg gtcattttgc cggtaagttt cagtttcaga
     6001 tcatccgcct gatactgctt ctcaccagtg aacggccagt aaagctgttt gctgttcact
     6061 tccacgttga gtggcaatcc ggcttccgcc agtcgcgtct gggcgcgtaa atccatatcc
     6121 accggaccgg aaaggttaac gcctatctcc agctgttcgc gcagcgcgcc gcccactttc
     6181 agcttcacct tttcaccttt caacggctcc acgttcagtg tgctgttgag ggtgatatcc
     6241 accggccagt tgtctgacag ctgcgccgtg ccgctggcgt tgacgatccc ctggttggaa
     6301 tcgatatcca gggcgtccag tttagtattg ccgtcaatgc tgctcacttt cagcagcatg
     6361 gtgcgcacgg tgatgtccgt gtcgcccgtc acgcgcagct gctcgccctt aaactcttca
     6421 atgttcaggt taagcggcaa atgcacgtcg gtcatttccg gcaataccgg gcgagaaaag
     6481 agatctttca gcgtttcgcc gagcggcttt tcctccggct gcggattttc aattttcggt
     6541 tcgaccactt cttcctgcgc cacttccacc actttcggca gagcaatcag caggcctttc
     6601 agcgacgtcg gtttcagggt cagggttttc tcctgccagt tcaggccgga ggtgaagtcc
     6661 atcaccgaca ccgtggtgtc atcaatcttg atgttgacgt tgtccagcgc cacccgtgtc
     6721 agggtgatgg gatacggcgt ggagagatcc agcggaccgc tatcttcctc ttcttcaacc
     6781 tgttcagaag gaggcatttt tttactgtcg atgttgacct gaatgtcttt cagcgccagg
     6841 tcattaatac aaacgctact gttccacagg cactcaagac cgacccccag atgcagatta
     6901 cccgctttta ccgccacgcc tagctgctca taacgaacgt cagacaaggt gagatcgcgc
     6961 cagccgccgg tgaccttgcc aatatccagt cctggcaccc agcgatccgc cgctttaaat
     7021 acaagatgca ggccgctggt ggtgcccacc agaaacgcca ccgatcccag caacagtaag
     7081 ataacgataa ccacgccgag gctgattttt ttccataaac tcataattct ggccccagac
     7141 cgatgtaaaa ctgtaacccg tgttcgtctt tatccgcgac cggtacggca aaatcgagtt
     7201 tgattggccc gaccggcgat tcccagcgta cgccgacccc ggtaccggtt ttaaagtcgc
     7261 tgcggcgaat atcgcttacc gcttcgccac tatcgacaaa caccgcgccc caccattttc
     7321 cggtcacgtt gtactggtat tccagcgatc cggttatcaa cttcgaggcc cctttcaggt
     7381 caccgttggc gtatttcggg gcgatagatt tgtatttgta gccgcgaata ctgcgatcgc
     7441 ccccgacgaa gaaacgcaga tccggcggta ctttgtcgaa atcaccggtt tcaatccagc
     7501 ccagcgtgcc gcgtgtcaca aagcgatggc gatcgtacag tgtgcggatc cagacgtgct
     7561 gcgcctggaa aacggagaaa tcgacatctg aaccccaggc cgtgttggag tagtcgatag
     7621 agtagcgttg cgagtctccc caggttggca tcaggccacc acgagaacgc gtgcggctaa
     7681 tcatcacccc aggataaaac agcatcgtgg tgttggtaat ttcaccctgg gtaaagtggt
     7741 cgaggctcca gcgcaggtta atggcacgct gccagccgct ggagagatcc cagtagcgag
     7801 aagccaccag cgtagtggag tcagattcgg tatcgttcag gtcagtgcgc ttaaaaccgc
     7861 cctgcaccaa ataatattgt tccagtggat tcttcagcag cggcatttta tagctgaagt
     7921 cgagggtctg ttccggcgcg gaaatactgg tactggtggt caaactgtga ccgtatgagt
     7981 tcatccacgg ctttttccac gtcgctttca cgcgcggtcc cacgtccgta gagtaaccga
     8041 ccccggtttc gatggtgttt tctgttcgcg gcgaaaccac gcccgtcaat ggtaatactt
     8101 tcgtttcgcg cgctttatca aattgtggag ccaccaccac cgagttaaac cagccggtag
     8161 cagaaagtcg gcggttcagt tccgccagat ctttcgattc atactcatcg ccctctttaa
     8221 acggcaccag attttgcagg tattcatcgc ggatttgtga tccttcaaag gtcacatgcc
     8281 caaagcggta acgttcgcca ctgttataat caatatccca gaaggcttta tgcaggccga
     8341 gcgcaatgcc cagctgcgct ttggtaaatt cgctatcgaa ataaccttta cgcaacgcaa
     8401 tactggttaa ggactttttg aaattttcat aatcgccctg gttcagtacc gtaccaatag
     8461 ccgggcgagt atcgagcaat ttcaaatagt ctttatcggt ccgcgcgccg ccgcgcaata
     8521 ccacatcggt gccgccaatt aacaccggca cacctggcgt gactttggcg atcaatacct
     8581 gccgcccttt ctttggcggt ggacggagat caaattcaat ggtcggctgg taataaccca
     8641 gcgctttcag accttcacgg atggcatcat cgacgcgtgc gcgaaagcga cggtctggcg
     8701 tcacttcatc actttcaatc gtagaaagct gcgcacgaac gttcttttcc agctgtcccg
     8761 ataacccctc gacctgtaga cggacgttcg cggcgacggc agatccgctt aagcagagta
     8821 agcttacaca gcataactgt cggatatagc gcacattttc tcctgaatat ccttttttcc
     8881 tgcccctgga acgccgttaa acggcttaac aaaaatccag taatatggat taaaaagcag
     8941 actaaacccc aaatatttct tatgttttac tttagaccta ttcacggtgg ttattgtgtg
     9001 caaatacgcc tcttgttaca accttaaccc caatgaccga ttttcgggag agcgacacca
     9061 tgagtttatt tgataaaaag catctggttt cccccgccga tgccctgcct ggacgtaaca
     9121 ccccgatgcc cgtagccacg ctgcatgcgg tcaacggtca ctcaatgacc aatgtacctg
     9181 acggaatgga gattgccatt tttgcgatgg gttgtttctg gggtgtggag cgtctgttct
     9241 ggcagttacc cggcgtttac agcaccgccg caggctatac cggcggctat acgccaaatc
     9301 cgacttatcg ggaagtgtgc tccggtgata cgggtcatgc cgaagcggta cgcattgttt
     9361 acgatccttc cgtcatcagc tatgagcagt tgctacaggt attttgggag aatcacgatc
     9421 ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca gtatcgttca gcgatttatc
     9481 cgctgacccc agaacaggat gccgcagctc gcgccagtct ggaacgtttt caggcggcga
     9541 tgcttgccgc cgatgacgat cgtcacatca ccacggaaat cgctaacgcc acaccgtttt
     9601 attatgccga agatgaccac cagcaatatc tgcataaaaa cccatatggt tattgtggaa
     9661 ttggcggaat tggcgtctgt ctgccgccgg aagcatagcg ttacgggtac aaatgtagtt
     9721 tgttgataaa gtgctctttg tctatgcctg atgcggctta aacgacttac caggcctaca
     9781 aaatcgccca aattcaatat attgcaagga ctgcgtaggc ctgatcggca tagcgcatca
     9841 ggcagtattg catttatcag cagtctgaat tttaaccctc tggcgacttt acagtacctt
     9901 acgctatact agccactgaa aatgccggtt cactttcttc gaatcggctt tcaatgtgta
     9961 tttcacacaa attaatcaac tttcccttcc gagggtctgg cctgaaaggt cggataagat
    10021 atgttaaaca gtattttagt catactctgc ttgatcgctg taagtgcgtt cttctcgatg
    10081 tccgagatct cgcttgccgc ctcacgcaaa atcaaactta aactgctggc tgatgaaggc
    10141 aatataaatg cccaacgcgt tctgaatatg caggaaaatc ccggcatgtt ctttaccgtg
    10201 gtccaaatcg gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga tgcggcattt
    10261 tctccagctt ttcacagcct gttctcccgc tatatgtcgg cagagctctc tgagcaactg
    10321 agctttattc tctctttctc gttagtgact ggcatgttta tcctgtttgc ggatttaacc
    10381 ccgaaacgca tcggtatgat tgcgccagaa gcggtggctt tgcgtatcat caacccgatg
    10441 cgcttctgcc tgtacgtttg caccccgctg gtgtggttct tcaacggcct ggcgaacatg
    10501 atcttccgta ttttcaaact gccaatggta cgtaaagatg acatcacttc tgatgacatc
    10561 tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga acacgagctg
    10621 attgaaaacg tctttgagct ggaatcccgt accgttccgt cttcaatgac accgcgtgaa
    10681 aacgtgattt ggtttgatct ccacgaagat gagcaaagcc tgaagaataa ggtggcggaa
    10741 catccgcact ctaagttcct cgtctgtaat gaagatattg accacatcat cggttatgtc
    10801 gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact gaacagcggc
    10861 gtacaaattc gcaacacgct gattgtgccg gatacgttaa ccctttcaga ggcgttggaa
    10921 agttttaaaa ccgcaggtga agacttcgcg gtgatcatga acgagtacgc gctggtggtg
    10981 gggatcatca ccctcaacga cgtgatgacc acgctgatgg gcgatctggt cggtcagggg
    11041 ctggaagagc agattgtcgc ccgtgatgag aactcatggc tgattgacgg cggcacccca
    11101 attgacgacg tcatgcgcgt gctggatatt gacgagttcc cgcagtcggg caactacgaa
    11161 accatcggcg gctttatgat gtttatgctg cgtaagatcc cgaaacgcac cgattcggtg
    11221 aaattcgccg gctacaaatt tgaagtggtg gatatcgata actaccgcat cgaccagctg
    11281 ctggtgaccc ggatcgacag caaggccacc gccctttcgc caaaactgcc tgacgctaaa
    11341 gataaagaag aaagcgtcgc gtaaccccca gaaacatcaa cggctcctga atcaggagcc
    11401 gtcttattac tgcatagcac tttggttaag ccatctctgt ttgcagacgc ataacctgac
    11461 ggttaacttc ggacatcact gacaaatgca gcttatcctt cacttttggg ataagaatct
    11521 tacccttatc aaattcaaaa gcgccaatgt ccttgatgta taaccgtcca cggaacagga
    11581 tctttacgta cttcgccacc tgaagtgggt tgtaccgttg gaaaattttc attgttatct
    11641 cctgctgagt attacgcctg tgcggtgcca caatcggccc aactattatg aggcgcaaat
    11701 tttaatgctt agtgactata gactatccgg gcaatgtttc caccgcgtat aacttttttt
    11761 acctaaaggt tacaattatt cagaattatc tttttaccga agcgcggtct tcagtataag
    11821 cattcatttt tcatatgatt tgtgcgcttg accgcaaact ggcatcacac ttgcgggaaa
    11881 ttcgataaat agcacatatg attaaaactc agacccaagt ggtcggatca cctgcatatc
    11941 ataagaagga aacaccatga ccctacgcaa gattctggca ctcacctgcc tgctgttgcc
    12001 gatgatggct tccgcacatc agttcgaaat cggtcagcga gtgccgccga ttggcattac
    12061 cgatcggggc gagttggtgc ttgataaaga tcagtttagc tacaaaacct ggaacagcgc
    12121 gcagttagtg ggaaaagtgc gagtactgca acatattgct ggtcgcacct ctgcaaaaga
    12181 gaaaaacgcg acgctgattg aagcgattaa atcagcgaag ttaccgcacg atcgttacca
    12241 gaccaccacc attgttaaca ccgacgacgc gattccgggt tccggcatgt ttgtgcgcag
    12301 cagtctggag agtaataaaa agctttatcc ctggtcgcag tttattgtcg atagcaatgg
    12361 cgtcgcgcgc ggtgcctggc agctggatga agaaagttcc gctgtagtgg tgctggataa
    12421 agacggtcgc gtgcaatggg ccaaagacgg ggcgctcaca ccggaagagg tgcagcaagt
    12481 gatggacctg ctgcaaaagt tgctcaaata atggcaataa cataaattct ggaaaaataa
    12541 ttttccccta tcgttatcta tgggataggg gaattaagtc tctgagttct tacaccgaac
    12601 taatactggt aacgctagtt gctctttttt aaacgcctca atggtcgatt tatatttctc
    12661 gcaaatcttt tctttggtaa tattactatc tgcggccaga ttcgcactta ctatcgcatc
    12721 cacttgttcg gctaattcga cttgttggca acctgcatta accagaaaat tataaataag
    12781 agagtaatct tcatcctcca aaacaacaga ggaaccatta cgaaaacgtt catcccctcc
    12841 atagatttta agcgcaaagc ccaaagattc tatgactatg gcaaaattat catcaattat
    12901 tttttcgtaa atataccatc catcgcattc tttaattcca ccgcctggta tttggagttc
    12961 aaatttaccc cgttttaatt tgcttactac atcctccaat aaggcataac cgaaagatac
    13021 gtcaccaaac gcctggcgaa tataaccttt aatccttttt ggtatgtcag actgtttttt
    13081 ctgcaaaata tcacaggcat acaatcttct gttttgttct aacggaaact cgcggttttt
    13141 gaaatccatg aatgagttat actcatttat tgttatggga atttcttcgt attcaaaccc
    13201 actgcctcgc aatgttattg tatattcagc accgttatct ttaaggctga aactgataag
    13261 aaaattatcc atggcattaa cagtttcatt ttttgatgaa agcacttgat aactattttt
    13321 atcaatctgt gttgcgcaac gaaaggactc ccgcaaagtt aacagactat cataattcga
    13381 aacccaatct tttttcttaa atgcatgctg aatattatta aaaaaatcgt ttgaatgtgt
    13441 ttttgtatgc gatgacttta caaataactc caccgcacca ataaaattat tgtgtctgat
    13501 cctgggtaac atatggctac cttattttat ttggttattt tatgtcgcca ttaaatatct
    13561 cattatagaa gtaaactatc aaaaaacgct cattattaaa atgtaactta ctagcaattg
    13621 aatttttgca ctttaaatac aatctctttt tatttataac tttttaggtt gggataaatc
    13681 aggatggcag aatcaggaaa tacactgttt ctgccatctg aatttagtaa atggacactc
    13741 tgaaccccgg attcaggaac gactcgcgcg gagtgtaatc cagcggttta ccctgccagt
    13801 cgtgaacgtg cgctccggca gctgcagcca cagcatgtcc ggcggcggtg tcccaaatat
    13861 tcgttggtcc gaagcgcggg tacagctgcg cctgtccttc cgccaccagg cagaatttca
    13921 gcgaagagcc gatggacgtg gtctgatgtt cgccaagctg ttgcagatac tctttcagct
    13981 ccgcatctgc atgggaacgg ctgataacca ccagcggcgg gcgcgcatcg cggacctgaa
    14041 tcagcttgcg cacaccgcac tcttctttcc aggccttgcc ttctgccgcg ctgtacatta
    14101 cgttcattac cggcgcatac accacaccta aaatcggttt gccatggtca atgagcgcaa
    14161 tgttaacggt gaattcgcca ttacgtttaa taaactcttt agtaccatcc agcgggtcta
    14221 ccagccagta acgctgccag tgctgacgga cttcccaacc gggaggatct tcttcagaaa
    14281 ggaccggaat atccggtgcc agcgtacgta aaccgtccat gataacggtg tgagcggcaa
    14341 tatccgctgc cgttaccgga gaattgtccg ctttgctgac gacgtccatc ggtttcgtcc
    14401 cgtcgtagac ctgcataatg gcatcgcctg cattccgtgc aagctggcat acttgatcta
    14461 acatttctcc acctcgtctc tgtgagcggt gttaacttat tgttttactt ataccctatc
    14521 gttaatgaat gcgccaactg tgatagtgtc atcattttca aagcgtaaaa ttgtggcatt
    14581 cttcactgtt ctataagtaa ggcgtttatt ctcccttttc tttcgtattc ccgatgataa
    14641 aaggatgtcc ctgatgatta agtttagcgc aacgctcctg gccacgctga ttgccgccag
    14701 tgtgaatgca gcgacggtcg atctgcgtat catggaaacc actgatctgc atagcaacat
    14761 gatggatttc gattattaca aagacaccgc cacggaaaaa ttcggactgg tacgtacggc
    14821 aagcctgatt aacgatgccc gcaatgaagt gaaaaacagc gtactggtcg ataacggcga
    14881 tttgattcag gggagtccgc tggccgatta catgtcggcg aaaggattaa aagcaggtga
    14941 tgttcatccg gtttataagg cgctgaatac gctggattat acggtcggta cactcggcaa
    15001 tcatgaattt aactacggtc tggattacct gaaaaatgcg ctggcgggag cgaaattccc
    15061 ttatgtaaat gccaacgtca ttgacgccag aaccaaacag ccaatgttta caccgtattt
    15121 aattaaagat accgaagtgg tcgataaaga cggaaaaaaa cagacgctga agattggcta
    15181 tattggcgtc gtgccgccgc aaatcatggg ctgggataaa gctaatttat ccggaaaagt
    15241 gacggtgaat gatattaccg aaaccgtgcg caaatacgtg cctgaaatgc gcgagaaagg
    15301 tgccgatgtc attgtcgttc tggcgcattc cgggctgtct gccgatccgt ataaagtaat
    15361 ggcggaaaac tcagtttatt acctcagtga aattccgggc gttaacgcca ttatgtttgg
    15421 ccatgctcac gccgttttcc cgggtaaaga ttttgctgat atcgaagggg ctgatatcac
    15481 caaaggcacg ctgaatggtg ttccggcggt aatgccgggc atgtggggcg atcatcttgg
    15541 ggtggtcgac ttacaactca gtaatgacag cggtaaatgg caggtgacgc aggcgaaagc
    15601 ggaagcacgg ccgatttacg acatcgccaa taaaaaatcc ctcgcggcgg aagacagcaa
    15661 gctggtagaa acactcaaag ccgatcacga tgccacacgc cagttcgtca gcaagccaat
    15721 cggtaaatct gccgacaata tgtatagcta tctggcgctg gtgcaggacg atccgaccgt
    15781 gcaagtcgtg aacaacgcgc aaaaagcgta tgtcgaacat tacattcagg gcgatccgga
    15841 tctggcaaaa ctgccggtgc tttcagctgc cgcaccgttt aaagttggtg gtcgcaaaaa
    15901 tgacccggca agctatgtgg aggtggaaaa aggccagttg accttccgta atgccgccga
    15961 tctttatctc taccccaata cgctgattgt agtgaaagcc agcggtaaag aggtgaaaga
    16021 gtggctggag tgctccgccg gacagtttaa ccagattgct cctaacagca cgaaaccaca
    16081 gtcactcatc aactgggatg gtttccgcac ttataacttt gatgttattg atggtgtgaa
    16141 ttatcagatt gatgttaccc aacccgcccg ctatgacggc gagtgccaga tgattaatgc
    16201 caatgcagag cggattaaga acctgacctt taatggcaag ccgattgatc cgaacgctat
    16261 gttcctggtt gccaccaata actatcgcgc ttacggcggc aaatttgccg gtacgggcga
    16321 cagccatatc gcttttgctt caccggatga gaaccgctcg gtgctggcag cgtggattgc
    16381 tgatgagtcg aaacgtgcgg gggaaattca tccggcggca gataacaact ggcgtttagc
    16441 accgatagct gccgataaga aactggatat ccgtttcgaa acttccccgt cagataaagc
    16501 cgcagcgttt attaaagaga aagggcagta tccgatgaat aaagtcgcga ccgatgatat
    16561 cgggtttgcg atttatcagg tggatttgag taagtaaaac acttcttttt cggcctataa
    16621 atcaaccgca tccggcattt attggcggat gcgatgctgc cgtatcttat ccaccctaca
    16681 agccatgaac cgtagaccgg ataagcgcag cgcatccggc agttatgccg cacgttcatc
    16741 ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag tccgccagtg cagcaacctt
    16801 ttcgctcacc tgctcgccca gcggcgtgag gctatattcc acatgcggcg gcaccaccgg
    16861 atacgcgata cggttaagaa aaccatcctg ttctaacgcc tgtaacgact gcgcaagcat
    16921 cttttcactc accccaccga ttttgcgccg caggtcgcta aagcgatgag taccttcgcg
    16981 tagcgccacc agaatcaaca ccccccaacg gctggtgacg tgtttcaaca cctcgcgcga
    17041 cgggcactgt tccgcaaaga gattaccctc tttcagttgt tgtgacaggc taacctgact
    17101 catttcatac ttaccttttt gcacgtactt actaaaagta agtttaggtg ttagcgtatt
    17161 taaacacaag acaaaacgat ggagacttcc catgatcgct attactggtg ccactggcca
    17221 acttggtcac tatattattg aatccttgat gaaaacggtt cctgccagcc aaatagtggc
    17281 tatcgttcgt aatccggcaa aagcccaggc cctggcagca caaggcatta ccgtgcgtca
    17341 ggctgactac agcgatgaag ccgcactgac atctgcactt cagggagtgg aaaaactact
    17401 gctgatctct tccagcgaag tgggtcaacg tgccccgcag catcgtaatg ttattaatgc
    17461 cgcaaaggcg gctggcgtga aatttatcgc ttataccagc ctgctacatg cagatacctc
    17521 cccgctcggc ctcgccgatg agcacatcga gacggagaaa atgctggctg attctggcat
    17581 cgtttacacc ctgctgcgca acggctggta cagcgaaaac tacctcgcca gcgccccggc
    17641 agcactggaa cacggcgtat ttatcggtgc ggctggcgat ggcaaaatcg cctcagcaac
    17701 gcgggcagat tatgcgacag ctgcggcacg cgtgattagc gaagccggtc acgaaggcaa
    17761 ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca cagttagcgg cagagctgac
    17821 caaacagagc ggcaaacagg ttacctatca aaatctgagc gaagccgatt tcgccgcggc
    17881 actgaaaagc gtcggactgc ccgacggact ggcggatatg ctggcggatt ctgacgttgg
    17941 cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt agcaaattga ttggctgccc
    18001 aacgacaacg ttagccgaaa gcgtaagcca tctttttaat gttaataact agttaattaa
    18061 agtggcatcc tcccgcatcc tctctgataa tgacgggatg ccgggagcaa tcatgtctgc
    18121 ttcctgaacg tttcttctga cagaccaatg gatgccagta atgattagcg gcgtgctgta
    18181 cgccctgtta gcagggttga tgtgggggct tatttttgtc gggccgttga tcgtgccgga
    18241 atacccggcg atgttgcagt cgatggggcg ttatctggcg ttagggttaa ttgcgctacc
    18301 cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg cgtcgggact ggcttaccgc
    18361 cttgatgctc actatgatgg gcaacctcat ttattacttc tgccttgcca gtgccattca
    18421 acgtactggc gcgcctgttt ccacgatgat tatcggcacc ctgccggtgg tgattcctgt
    18481 ctttgccaat ctgctttata gccagcgcga cggcaaactc gcgtggggaa aactcgcccc
    18541 ggcactgatt tgtattggca tcggcctggc gagtgtgaat attgctgagt taaaccacgg
    18601 actccccgat tttgactggg cacgttatac ctctggcatc gtgctagcgt tagtttccgt
    18661 ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg ctgcgggaaa atcccgacaa
    18721 acatccgatg atgtgggcga cggcgcaggc gctggtcacg ctgccggttt ctctcatcgg
    18781 ctatctcgtc gcctgttact ggctgaatat acaaacgccg gacttctcct taccttttgg
    18841 cccccgtccg ctggtgttta ttagtctgat ggttgcgata gccgtgcttt gctcatgggt
    18901 tggcgcactc tgctggaacg tcgccagcca gcgattaccg acagtgattc tcgggccgct
    18961 gattgttttc gaaacgctgg caggtttgct gtacaccttt ttactccgcc agcaaatgcc
    19021 gccgctaatg acgctgagcg gtatcgcgct gttagtgatt ggcgtggtca tcgcggtcag
    19081 agcaaaacca gaaaaacctt taactgaatc tgtctcagaa agttgacatg ctggcagtga
    19141 gttaaataag cctctgctac gtaagggtta tagcttttgc cttaaaggtg tatttaaaat
    19201 acatcttatc ttattaagaa tgaggtatca gctatggctt atcgcgacca acctttaggt
    19261 gaactggcgc tctctatccc tcgcgcttcg gctctgtttc gtaaatatga tatggattac
    19321 tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac gtaaagaact ggatgttgag
    19381 gtcattgaag ctgaactggc aaagcttgct gaacaaccga ttgagaaaga ctggcgtagc
    19441 gccccgctgg cagaaatcat tgaccatatc atcgtgcgct accacgatcg tcaccgcgag
    19501 cagctgccgg agctgatcct gcaagcgact aaagtcgagc gcgttcacgc cgacaaaccg
    19561 agcgtgccaa aagggctgac aaaatacctg accatgctgc atgaagagct ttccagccac
    19621 atgatgaaag aagagcagat cctcttcccg atgatcaaac aaggcatggg cagccaggca
    19681 atggggccaa tcagcgtaat ggaaagcgag cacgatgaag cgggcgaact gctggaagtg
    19741 atcaaacaca ctaccaataa cgtcacaccg ccgccagaag cctgcaccac ctggaaagcg
    19801 atgtataacg gcattaatga actgattgat gacctgatgg atcacatcag tctggaaaac
    19861 aatgtactgt tcccacgcgc cctagcgggt gagtgatcta aaagctggat ggcattgcgc
    19921 catccagcat gataatgcgg ttatttccgc agttcagcag cccgcttctt accaataaac
    19981 agccagccca accccagcgc gataaaccac agcggggtaa ccagcagcgc ctggcgagtg
    20041 tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga acgccataca tacccagcac
    20101 atcagcttgc cgagcggcat cttgtagatc gacttttcat gcagatgagg acgctgtttg
    20161 cggtacacca ggtaagagca aaggataatc gtccagacga acataaacag aatcgcggaa
    20221 acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat tcacatacaa catcaccacg
    20281 ccgccgagca gacagataca cgagaaggtc aaccctttcg cgggtactgc gcgcttagaa
    20341 agtttagcga acgctttcgg cgcaacacct tcctgcgcca gaccaaacag catacggctg
    20401 gtagagaaga caccgctgtt agcggaagac gccgcagagg tcagcaccac aaagttgatc
    20461 acgctggcag ccgcaggcaa acctaccaac acaaacagct caacgaacgg gcttttctcc
    20521 gggactaccg aactccacgg tgtcacggac ataatcacaa tcagcgcgaa gacgtagaac
    20581 ataatgatac ggatcggaat ggagttaatc gcgcgtggca gtgatttctc cggatctttg
    20641 gtttccgcag ctgttgtacc taccagctca atccccacga aagcgaaaac cgctatctgg
    20701 aatccggcaa agaagccact taaacctttc gggaaccagc cgccgtcatt ccacaaatgc
    20761 gcgaatgacg cttccacacc ggtcggtgac tgaaagtgca tcgccaccat gaccaggccg
    20821 acgacaatca gcgacacgat ggcgacgatt ttgatcatcg caaaccagaa ctccatctca
    20881 ccgaacattt tcacggtggc aaggttgagc gtcagcagca gcactatcac cgccagcgag
    20941 gcgacccagt cggagagatc ggggaaccag aactgagcat aagccgtgat cgccaccacg
    21001 tctgccatac cggttacaac ccagcagaac cagtaagtcc agccggtgaa atatcccgcc
    21061 cacggcccca gtaaatcgga agcgaagtca ctaaaagatt tgtattccag attcgaaagc
    21121 agcaattccc ccattgcccg catcacgaaa aagagcataa aaccaatgat catataaacg
    21181 aaaatgatcg acggcccggc aaggctaatc gttttgccgg accccataaa caacccagta
    21241 ccaatggcac ctccaatggc aataagctga acatgtcggt ttgtgagatt gcgccgtagc
    21301 gactgttcag ccggagcctg atcatcggca acgactttta cctgatctac catgtttttt
    21361 cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt tagtcagtct atgatacgac
    21421 gaatctgtat cgggactcat cgatattagg taacaatacg cgggatgaat actaagattt
    21481 agatctaatg ttaattttat gtttaaagtg agtgtcatat cacggtgata acgcgaaaca
    21541 gctcacaaaa atacacgctt acggggtctt tgcaacacaa aatagcgccg tttggcaaac
    21601 tgaagggttt attgctgaat gcctgctccc ctctggttcg aggggaacag aatgcgctgc
    21661 ttagaggatt tccagcagtt cgacttcaaa caccagggtg ctgaacggag ggatggatgc
    21721 acctgcgccg cgctcgccat atgccagttc ctgcgggata gtcagttccc atttagaacc
    21781 taccggcatc agagtcagtg cttcaatcca gccagggatc acgccattaa ccgggaattc
    21841 agcgggttca ccacgagcaa cggagctgtc aaacacggtg ccgtcgatca gtttaccggt
    21901 gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg ccttcaccct ggttgatcac
    21961 gcggaattgc aggccagatt cggtgctatt cacaccttct tttttggcgt tttcttccag
    22021 gtatttcacg ccttcagcag ccatcgcctg gaaacgctga cgacgaacgg catcggcgcg
    22081 ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc ggcacagccg gatgtttgcc
    22141 ttccagcgca tcggcaatac ctgcaaccag tgcttctggc agcagccctt ccaggccaga
    22201 ttcactcagt tgttgcccta cctgcaaacc aatgccgtag cttgcttgcg cttcgatggt
    22261 gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg tataaaagta gcgggcagca
    22321 tatcagccac acttctttgg gtaaaacttt gtcgcaggaa tgatgacaaa tcccggggaa
    22381 aaagaaacaa tagcaatatc ccgtatgaat ataataaagg cgggtttacc acggacatca
    22441 tagcgttagc ttattcatag gctatgattg aggaacaaga cgtggagcag gaggaaacca
    22501 tgcccgggcg ctttgaacta aaaccaaccc tggagaaagt ctggcacgcg ccggacaatt
    22561 ttcgcttcat ggacccgctg ccgccgatgc atcgtcgggg gatcattatt gccgccattg
    22621 tattggtggt cggatttctg cttccatctg atgatacgcc caacgcgtcg gtggtgacgc
    22681 gtgaagcgca gttagatatt cagtcgcaat cacaaccgcc gacagaagaa cagccgcaaa
    22741 ctacccaaac gcaaccattc cagccagata gcggaattga taatcaatgg cgctcttacc
    22801 gtgtcgaacc cggtaaaacg atggcgcagc tgttccgcga ccacggactg cccgctaccg
    22861 atgtctatgc aatggcgcag gtagaaggcg cgggtaagcc gctgagtaat ttgcaaaatg
    22921 ggcagatggt gaaaatccgc cagaacgcca gcggcgtggt gacgggctta accattgata
    22981 cggggaataa tcaacaggtc ctgtttaccc gccagccgga tggcagtttt attcgtgcgc
    23041 ggtagatgaa gggtcaacca aaagccagct aaggattgtc tctttgatga ccttacgtct
    23101 ggcttccagt gcctgggggg aaaccatact gatatgataa actaggccaa gcgtatgctg
    23161 gttgatcaaa taatatcccc caagcgcagc aatattaata ttcacctgca atgggtcgat
    23221 acctggctta aaaatattgt atttcttccc ttcatccaat aaggattcca ttaactgcaa
    23281 gtgagcatgg ttaatttcta agagacgctg tgattttgca taatgcacac ctttactttg
    23341 attttcacta tgcacaattt ttaaaaacca aggattagca atataataat cccatgtaaa
    23401 atcaataatt ttctcaatgg ctttttgtgg cggaagatct gagaaatcaa gggccttttc
    23461 tttttgtcga atatcgttcc aggtatattc cagcacttcg ataaataaat tttctttatt
    23521 accaaagtgg tgataaaccg tctgtttact acaccctgct gcctttacaa tattatctac
    23581 tctggcccct tcataaccat attcggcaaa ttcattaacc gcacatagca gcaatttttc
    23641 cttcaggctc tgtacgttgc tggtatcaat atacataacg cttccccacg acctcttcgt
    23701 ggtgactatc ataacctgat ttgatttcag cttcagaaaa aagggcaaaa acttgtactt
    23761 tcatcaggtt atcaaatgtc ctgtgatcgc catcgcatat caaccaacta gatggtttat
    23821 ctttttatcc tccagtcgca agctaatcat caccaaggaa acaaaattca aaaaagagag
    23881 ctattgtatg aaatccactc gtcctgttgc cgtgattacc ggagccactc gcggtattgg
    23941 aaagggctgt gcgcaggagc ttgctcgtgg cgggtttaac ctgctgatta acgatcgtcc
    24001 cgatgccgac agcgtagaaa aactgcatat cacacaacaa gaatgtttgg cggaaggcgt
    24061 ggaagtgatt tgcttccctg ctgatgtcgg tgatctatct ctgcatgagg agatgctcga
    24121 cgcggcgcaa aaccagtggg ggcggctgga ctgtctgctc aataacgcgg ggatttcagt
    24181 gaaaaaacgc ggtgacctcc tcgacctcga accagacagc ttcgatcaaa acattgccat
    24241 taacacccgc gcgccgttct tccttgctca ggcattcagt aagcgcttac tggcacagcc
    24301 gaaaccggaa gcggagctgc cgcaccgctc gattattttc gtcagttcca tcaacgccat
    24361 catgctggcg atgaatcgcg gcgaatacac cattgccaaa accgccgttt ctgccgctgc
    24421 tcgtctgttt gctgctcgcc tttgtaatga acaaattggc gtttatgaag tgcgccctgg
    24481 tctcatcaaa actgatatga ctattccggc aacggcttat tacgacgaac tgattgccaa
    24541 aggtctggtg ccgtggggac gttggggtta tccggctgat attgcttcca ccgtccgcgc
    24601 gatggcggaa ggtaaactga tttacacctg tggtcaggcg gtcgcaatcg acggcggctt
    24661 aagtatgccg cgcttctgag gctgaatcat gagagatctg ttacaacgcc cggatttatt
    24721 cagtatcaat accgcgacac tgggctataa aacgccgcta cccgccatta ttgatgcctg
    24781 cgccgcacgc ggtattggcg caattgcccc ctggcgcagg gagttgcagg gtgaagatct
    24841 ccagcaaatc acccgccagc ttgccgccag taacatgagc gtttccggtt tgtgccgtag
    24901 cacttactac accgcgccga cactggcgga acgcaaactg gcgattgatg ataaccgccg
    24961 ggcgctggac gacgcagcgg tgcttaacgc cgcctgttat atgcaggtgg ttggcggtct
    25021 gccaacgggc actaaagatc tatatgaagc gcgagaacaa gtgaaacaag ggattcgcca
    25081 gttgctgcca cattcgaaag atgtcggcgt gccaattgcg ctggagccac tgcacccgat
    25141 gacagctgcc gatcgctctt gcctgtgcac gttacgtcag gcgctggact ggtgtgatga
    25201 actcgatccc gatggtgagt ttggcctcgg cgtggcggtg gatgtttatc acgtctggtg
    25261 ggatccggat ctcgccagtc agatcctgcg tgccggaaaa cgcatcctcg cgtttcatgt
    25321 ttccgactgg ttaatgccga ccactgatct ggtcaatgac cgtggtatgc cgggagatgg
    25381 cgtcatcaat attccgtcaa ttcgtcggct ggttgaaaac gccggattta atggtgctat
    25441 tgaactggaa atattctcgc cttactggtg gcagaaagat attaacag
//
LOCUS       DAJTMX010000052        25212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTMX010000052 DAJTMX010000000
VERSION     DAJTMX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.052"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1108
                     /locus_tag="OO145_002094"
     CDS             <1..1108
                     /locus_tag="OO145_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR5467208.1"
                     /translation="AWSTVENVGIILLAVGVAMDGLSLHDPLLTVVGLLGALFHLLNH
                     ALFKGLLFLGADAIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISAIPPLNGFIS
                     EWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHA
                     EEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVP
                     GTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAWACGYGWENAMAPSG
                     NGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPFWDERVIRPIVSATQ
                     RLVKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            1119..2066
                     /gene="hyfC"
                     /locus_tag="OO145_002095"
     CDS             1119..2066
                     /gene="hyfC"
                     /locus_tag="OO145_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5467209.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITFIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            2083..3522
                     /gene="hyfD"
                     /locus_tag="OO145_002096"
     CDS             2083..3522
                     /gene="hyfD"
                     /locus_tag="OO145_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5467210.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            3534..4184
                     /gene="hyfE"
                     /locus_tag="OO145_002097"
     CDS             3534..4184
                     /gene="hyfE"
                     /locus_tag="OO145_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5467211.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            4189..5787
                     /gene="hyfF"
                     /locus_tag="OO145_002098"
     CDS             4189..5787
                     /gene="hyfF"
                     /locus_tag="OO145_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5467212.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVL
                     MAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLP
                     AQRSTWHDFLPSGTASVSEKHSER"
     gene            5777..7491
                     /gene="hyfG"
                     /locus_tag="OO145_002099"
                     /pseudo
     CDS             5777..7491
                     /gene="hyfG"
                     /locus_tag="OO145_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            7501..8046
                     /gene="hyfH"
                     /locus_tag="OO145_002100"
     CDS             7501..8046
                     /gene="hyfH"
                     /locus_tag="OO145_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5467213.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            8043..8801
                     /gene="hyfI"
                     /locus_tag="OO145_002101"
     CDS             8043..8801
                     /gene="hyfI"
                     /locus_tag="OO145_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5467214.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            8794..9207
                     /gene="hyfJ"
                     /locus_tag="OO145_002102"
     CDS             8794..9207
                     /gene="hyfJ"
                     /locus_tag="OO145_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5467215.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            9237..11066
                     /gene="hyfR"
                     /locus_tag="OO145_002103"
     CDS             9237..11066
                     /gene="hyfR"
                     /locus_tag="OO145_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5467216.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            11067..11249
                     /locus_tag="OO145_002104"
     CDS             11067..11249
                     /locus_tag="OO145_002104"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5467217.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            11271..12143
                     /locus_tag="OO145_002105"
                     /pseudo
     CDS             11271..12143
                     /locus_tag="OO145_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(12237..13298)
                     /locus_tag="OO145_002106"
     CDS             complement(12237..13298)
                     /locus_tag="OO145_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5467218.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            13511..14974
                     /gene="bepA"
                     /locus_tag="OO145_002107"
     CDS             13511..14974
                     /gene="bepA"
                     /locus_tag="OO145_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5467219.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            14995..15354
                     /gene="arsC"
                     /locus_tag="OO145_002108"
     CDS             14995..15354
                     /gene="arsC"
                     /locus_tag="OO145_002108"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5467220.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(15487..16233)
                     /gene="hda"
                     /locus_tag="OO145_002109"
     CDS             complement(15487..16233)
                     /gene="hda"
                     /locus_tag="OO145_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5467221.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(16283..17572)
                     /gene="uraA"
                     /locus_tag="OO145_002110"
     CDS             complement(16283..17572)
                     /gene="uraA"
                     /locus_tag="OO145_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5467222.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(17658..18284)
                     /gene="upp"
                     /locus_tag="OO145_002111"
     CDS             complement(17658..18284)
                     /gene="upp"
                     /locus_tag="OO145_002111"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5467223.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            18608..19645
                     /gene="purM"
                     /locus_tag="OO145_002112"
     CDS             18608..19645
                     /gene="purM"
                     /locus_tag="OO145_002112"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5467224.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            19645..20283
                     /gene="purN"
                     /locus_tag="OO145_002113"
     CDS             19645..20283
                     /gene="purN"
                     /locus_tag="OO145_002113"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5467225.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            20455..22521
                     /gene="ppk1"
                     /locus_tag="OO145_002114"
     CDS             20455..22521
                     /gene="ppk1"
                     /locus_tag="OO145_002114"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5467226.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            22526..24067
                     /gene="ppx"
                     /locus_tag="OO145_002115"
     CDS             22526..24067
                     /gene="ppx"
                     /locus_tag="OO145_002115"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5467227.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(24106..>25212)
                     /gene="pdeF"
                     /locus_tag="OO145_002116"
     CDS             complement(24106..>25212)
                     /gene="pdeF"
                     /locus_tag="OO145_002116"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5467228.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         5817 a         6584 c         6653 g         6158 t
ORIGIN      
        1 ggcctggagt accgtcgaaa acgtcggcat tattttgctg gcagtcggtg tggcgatgga
       61 cggtctgtca ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg cactgtttca
      121 tctgctcaac catgcgctgt tcaaagggct gctatttctc ggcgcggacg cgattatttc
      181 gcgtttgcat acccacgaca tggaaaaaat gggggcactg gcgaaacgga tgccgtggac
      241 agccgcagca tgcctgattg gttgcctcgc gatatcagcc attcctccgc tgaatggttt
      301 tatcagcgaa tggtacacct ggcagtcgct gttctcacta agtcgtgtgg aagccgtagc
      361 gctacaactt gcgggtccta ttgctatggt aatgctggca gtcactggtg ggctggcagt
      421 aatgtgcttc gtcaaaatgt acggtattac tttctgtggt gcgccgcgca gtacacacgc
      481 tgaagaggca caggaagtgc caaatacgat gatcgtcgcc atgctactgc tcgcggcact
      541 ctgcgtatta attgcgctta gtgccagttg gctggcaccg aagataatgc atattgccca
      601 tgcgtttacc aatacccctc ccgtcactgt cgccagcgga atagcacttg tacccggcac
      661 gtttcataca cgggtcactc cttcattact gttgctgtta ctactggcga tgcctttgct
      721 gccaggcctt tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc gcacaggaga
      781 tgcctgggca tgcggctacg gctgggaaaa cgcgatggct ccgtcaggca atggcgtgat
      841 gcagccgctg cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc tcgaccctac
      901 gctgaggcta aataaaggtc ttgcgcacgt caccgccagg gctcagagca cagaaccctt
      961 ctgggatgag cgggtgatcc gccccatcgt gagcgccacc caacggctgg tcaaagaaat
     1021 acagcatctg caaagcggcg actttcgtct ctattgcctg tatgtggtcg ccgcactggt
     1081 tgtgctgcta atcgctattg ccgtctaagg aaatcaccat gagacaaact ctttgcgacg
     1141 gatatctggt catttttgcg ttagcacagg ccgtgattct gctgatgcta accccacttt
     1201 ttacgggtat ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg ccggggatct
     1261 ggcaggatta tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg ccgacatctt
     1321 caggtctgat gttccgcctg atgccgtggg tattaatcag cagcatgctg gtgctggcga
     1381 tggccttacc actatttatt accgtttccc cttttgcggg cggcggcgat ctgatcacct
     1441 ttatctatct tcttgccctg tttcgttttt tctttgctct ttccgggctg gataccggaa
     1501 gtccgtttgc gggagtcggt gccagtcgcg agttgacgct cggcattctg gtcgaaccaa
     1561 tgcttattct ctcactgctg gtattggcgc tgatagcagg ttccacgcat atcgagatga
     1621 tcagcaatac gctggctatg ggctggaact cgccgctaac caccgtactg gcgttactgg
     1681 cctgtggttt tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt gctgaagcag
     1741 aacaggaatt acaggaaggc ccgctgaccg aatattccgg tgccgggctg gcgctagcga
     1801 aatgggggct ggggctgaaa caggtcgtga tggcagcact gtttgtggcc ctgtttctgc
     1861 cctttgggcg cgcgcaagaa ctttctctcg cctgcctgct gacttcactt gtcgttacgc
     1921 tgctcaaggt tttgctgatt tttgtactgg cctcaatcgc agaaaacacg ctggcacgcg
     1981 ggcgtttttt actcattcac catgtgacct ggcttggctt cagccttgct gcgcttgcat
     2041 gggtcttttg gttaaccggt ctgtaaggag cactgacgga atatggaaaa tcttgctctg
     2101 acgacgttaa tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc acaacgtcgg
     2161 gccgccgaat ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt gtcgctgatc
     2221 tccgcgtttt atcaggccga taaagttgcc gtcacgttga cgttggtcaa cgtgggggat
     2281 gtggcattat ttggcctggt cattgatcgc gtgagtacgc tgattctgtt tgtggtggtg
     2341 tttctcggtt tgctggtcac gatctactcc acgggttatc tgacggataa aaatcgcgaa
     2401 cacccgcata acggcacgaa tcgttattac gcatttttgc tggtgtttat cggcgcgatg
     2461 gcgggactgg tactctcctc gacgctgctc ggtcagttgt tgttttttga aattacgggc
     2521 ggctgctcct gggcgttgat cagttattac cagagcgata aagcgcagcg ttcagcacta
     2581 aaagcgttgc ttatcaccca tatcggttcg ttggggttgt atcttgccgc cgccacgctg
     2641 tttttgcaga ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt
     2701 tatctggttt atggcggcat cctgtttgcc gcgtggggga aatcggccca gctaccgatg
     2761 caagcgtggc taccggacgc aatggaagcg ccaacaccga tcagcgccta tctccacgcc
     2821 gcatcgatgg tgaaagtggg cgtttacatt tttgcccgcg ccattatcga cggcggcaat
     2881 atcccgcatg tgattggcgg cgttggcatg gtcatgacac tggtcaccat tctttatggc
     2941 tttctgatgt atttgccaca gcaggatatg aagcggttgc tggcctggtc gaccatcact
     3001 caacttggct ggatgttctt cggcttgtcg ctctccatct tcggctcgcg gctggcgctg
     3061 gagggtagca tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt tttccttgta
     3121 gcaggtgcgc tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg tggcgtattg
     3181 cacaccctgc cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat taccggtgtg
     3241 ccgccgttca acggcttctt cagtaaattc ccgctgtttg ccgccggttt tgcgttgtca
     3301 gtggagtact ggatcctgct gcccgccatg attctgctga tgattgaatc ggtcgccagt
     3361 ttcgcctggt ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag cgaggccgtc
     3421 gccgatgccg caccgctgcc agggtcaatg cgcctggtgt tgattgtact gattgtgatg
     3481 tcgctgattt ccagcgtaat cgccgcgacc tggttgcagt aaggagatga tgaatgaccg
     3541 gttctatgat cgtaaataat ctggcgggac tgatgatgct gacatcgctg tttgtgatta
     3601 gcgtcaaaag ctatcgcctg tcatgcggat tttacgcctg ccagtcgctg gtgctggtgt
     3661 ctattttcgc cactctctcg tgcctgttcg ccgcagagca actgctgatc tggtccgcca
     3721 gcgcctttat caccaaagtg ctgctggtac cgttaatcat gacttacgct gcacgaaata
     3781 ttccccagaa catcccggaa aaagcgttat tcggtccggc aatgatggca ctgctcgcgg
     3841 cgttaattgt cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg atggctaccg
     3901 ggctgaaacc ggcgctggcg gtagcgttag gtcattttct gcttggcctg ctgtgcattg
     3961 tcagccaacg caatatcctg cggcaaattt ttggttactg cctgatggaa aacggctccc
     4021 atctggtgct ggcgcttctt gcctggcgag caccggaact ggtggaaata ggtatcgcta
     4081 ccgacgccat cttcgccgtc attgtgatgg tgttactggc aagaaaaata tggcgtaccc
     4141 acggcacgct ggacgtgaac aacttgaccg cgctgaaggg ataatgagat gagttattct
     4201 gtgatgttcg ctttactcct gctcacgccg ctgctttttt cgctgctctg ttttgcctgc
     4261 cggaaacggg ggctctctgc gacttgtacg gtgactgtat tacatagctt agggatcaca
     4321 ctgctgctga ttctagcact ctgggtggtc caaactgccg ctgatgcagg agaaatattc
     4381 gctgcgggac tgtggcttca tattgatggt ctgggcggtt tgttcctcgc cattcttggt
     4441 gtgattggct ttctcaccgg tgtttactcg attggctaca tgcgtcatga agtggcacac
     4501 ggcgagcttt cacccgttac actgtgcgat tactacggtt tcttccatct gtttttgttc
     4561 accatgctgc tggttgttac cagcaataac ctgattgtga tgtgggcggc gatcgaagcc
     4621 accaccttaa gctcggcgtt tctggtaggc atttacggtc agcgttcatc gctggaatca
     4681 gcatggaagt acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt cggtaccgtg
     4741 ctggtatacg ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat attctggagc
     4801 gaggttctta agcaatcgtc cttgcttgac ccaacattaa tgctgttggc ctttgtgttt
     4861 gtgctaattg gctttggtac caaaaccggg ctatttccca tgcacgcctg gctgccggat
     4921 gctcacagtg aagcgccgag tccggtcagc gccctgctct ccgccgtatt gctgaactgc
     4981 gcgctgttgg tgctgattcg ctattacatc attatttgcc aggccatcgg cagcgatttc
     5041 cccaaccggt tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc atttttcatt
     5101 ctggtacagc gggacattaa gcgtctgctg gcgtactcca gcgtggagaa catggggctg
     5161 gtcgcggtgg cgctaggcat tggcgggccg ctgggaattt ttgccgcgct gctgcacacc
     5221 ttaaaccaca gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt actgctcaag
     5281 tacggcacgc gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc atttaccgcc
     5341 gtgctgtttg gcggcggtgc gctggcgctg gcgctggcgc tggcgctggc agggatgccg
     5401 cccttcaaca tttttcttag cgaatttatg accgttaccg caggactggc acgtaatcac
     5461 ctgctgatta tcgtcctgct gttattgctg ttaacgctgg tgctggcggg cctggtacgg
     5521 atggctgcgc gggtgttaat ggcgaaaccg ccgcaggccg ttaaccgggg tgatctcggc
     5581 tggttgacca cctcgccaat ggtgattctg ctggtcatga tgctggcgat gggaacgcat
     5641 attccacaac ctgtcatcag gatcctggcg ggcgcttcca ctatagtcct ctcagggacg
     5701 cacgacctgc ctgcacaacg tagcacctgg catgattttt tgccttcagg caccgcatct
     5761 gtttcggaga aacacagtga acgttaattc atcgtcaaat cgtggcgaag cgattctcgc
     5821 cgccctgaaa acgcagttcc ccggcgcggt gctggatgaa gagcgacaaa cgcctgaaca
     5881 ggtcaccatt acggtgaaaa tcaatctgct gcctgacgtt gtacattatc tttattatca
     5941 acatgatggc tggcttccag tcctgtttgg caacgacgag cggacactta acggtcatta
     6001 cgcggtttat tatgcccttt caatggaagg ggccgaaaaa tgctggatcg tggtgaaggc
     6061 actggtcgat gccgacagtc gggagtttcc gtcagtcaca ccgcgcgtcc ctgccgcggt
     6121 ctggggcgag cgagaaattc gcgatatgta cgggctgatt ccggttggcc tgccggatca
     6181 gcgtcgcctg gtgttgcccg atgactggcc ggaagatatg catccgctgc gcaaagatgc
     6241 gatggattat cgactgcgcc ctgaaccgac gacagattcc gaaacgtatg cgtttatcaa
     6301 tgagggcaac agcgatgcgc gggtgatccc tgtcggcccg ctgcatatca cctcctatga
     6361 accgggtcac ttccgcttgt ttgtggatgg cgagcaaatt gtcgatgctg attaccgcct
     6421 gttttatgtc catcgcggca tggagaaact ggcagaaacg cggatgggct acaacgaagt
     6481 gaccttctta tcggaccgcg tgtgtgggat ttgcggtttt gcccacagtg tggcctatac
     6541 caactcggtt gaaaatgcac tggggattga ggtgccgcaa cgagcacata ctattcgctc
     6601 gattctgctg gaagtcgagc ggctacacag tcatttgctc aaccttggcc tctcctgcca
     6661 ttttgttggt tttgataccg gctttatgca atttttccgc gtgcgggaaa agtcgatgac
     6721 gatggcggaa ttgctgaccg ggtcgcgtaa aacctacggt ctgaatctga ttggtggtgt
     6781 tcgccgcgat attctcaaag agcaacgtct gcaaacgctg aaactggtgc gcgagatgcg
     6841 cgccgacgtg tcggagctgg tagagatgct gcttgctacg ccgaatatgg aacaacgcac
     6901 tcagggcatt ggcattctcg accgacaaat cgcccgtgat tatagccctg tagggccgct
     6961 gatccgcgca gtggttttgc ccgtgatttg cgctttgatc acccctacgc cgactacggc
     7021 aatattccaa aaacactgtt tacctttacc ggcggcgatg ttttctcccg cgtgatggtc
     7081 cgtgtcaaag agacgtttga ttcgctggca atgctggaat ttgccctcga caacatgccg
     7141 gataccccac tgctgaccga aggctttagc tataaacctc acgcattcgc gctgggcttt
     7201 gttgaagcgc cacgcggtga agacgtgcac tggagcatgc tcggtgataa ccaaaaattg
     7261 ttccgctggc gctgtcgtgc cgccacctac gccaactggc cggtgttgcg ttacatgctg
     7321 cacggcaata ccgtttctga cgcaccgctg attatcggta gccttgatcc ctgctactcc
     7381 tgtaccgacc gtgtgacgct ggtagatgtg cgcaagcgcc agtcaaaaac cgtgctgtat
     7441 aaagagatcg aacgctacgg cattgatcgt aaccgttcgc cgctgaagta aggacagaag
     7501 atgctgaagt tactgaaaac tattatgcgc gccggaaccg cgacggtgaa atatcccttc
     7561 gcgccactgg aggtcagccc aggctttcgc gggaaaccgg acctgatgcc cagccaatgt
     7621 attgcctgcg gtgcctgcgc ctgtgcttgt ccggcaaatg cgctgactat ccagaccgac
     7681 gaccagcaaa attcgcgcac ctggcagctc tatctggggc gttgtattta ctgcggacgt
     7741 tgtgaagaag tgtgcccgac cagagccatc cagcttacca ataactttga actgaccgtc
     7801 acccataaag ccgatctcta tacccacgcg acgttccatc tacaacgttg cagccgttgc
     7861 gaacgcccgt ttgccccgca aaaaaccgtc gcactggctg ctgaattgtt ggcacagcaa
     7921 caaaatgctc cacaaaaccg cgaaatgtta tgggcgcaag cgagcgtctg cccggagtgc
     7981 aaacaacgcg caacgctgat caacgacgat acagatgtac cgctggtggc taaggagcag
     8041 ctatgagtcc agtgcttacg caatatgtca gccagcctat cacgctggac gagcaaacgc
     8101 aaaagatgaa gcggcatttg ctacaggata tccgtcgctc ggcttacgtt tatcgcatcg
     8161 attgcggcgg ctgcaacgcc tgtgaaatcg aaatttttgc tgccatcacc cccgtattcg
     8221 acgcagaacg ttttggcatt aaggttgttt catcaccgcg tcacgccgat attttgttat
     8281 ttactggcgc agtcacccgg gcgatgcgta tgcctgcact tcgggcgtat gagtctgccc
     8341 ccgatcataa aatctgtgtt tcctacggcg cgtgcggtgt cggcggcggt attttccacg
     8401 atctctacag cgtctggggc ggcagcgaca ccattgtccc cattgatgtt tggatccccg
     8461 gctgcccgcc aacaccggcc gccaccattc acggtttcgc cgtggcgctc ggtttgctgc
     8521 aacagaagat tcacgcggtg gattatcgcg atcccaccgg ggtgactatg caaccgttgt
     8581 ggccgcagat cccgccatca cagcgtatcg ccattgagcg agaagcgcgg cggctggcgg
     8641 gctatcgtca ggggcgagaa atttgcgatc ggctcctgcg ccatttaagc gacgatccta
     8701 caggaaatcg ggttaacacc tggttgcgcg atgccgacga tccacgtctc aatagtatcg
     8761 ttcagcaact ctttcgcgta ctccgggggt tacatgactg aagagtgcgg ggaaattgtt
     8821 ttctggacgc tgcgaaaaaa gtttgtcgcc agtagcgacg agatgccgga acacagctct
     8881 caggtaatgt attactcgct ggctatcggt catcacgttg gcgtgattga ttgtctgaat
     8941 gtcgccttcc gctgcccact gacggaatac gaagattggc ttgcactggt cgaagaggag
     9001 caagcccgac gtaagatgct gggggtgatg acttttggtg agattgttat tgacgccagc
     9061 cacaccgccc tgttgacccg ggcattcgcg ccactggcag atgacgcgac gtctgtgtgg
     9121 caagcgcgta gcattcaatt cattcatctg ttggatgaaa ttgtgctgga accggctatc
     9181 tatctgatgg ccagaaaaat tgcgtgagaa ggatttctca ttaataagga ctgttgatgg
     9241 ctatgtcaga cgaagcgatg tttgccccgc cgcaaggaat aacaattgaa gcggtaaacg
     9301 gaatgctcgc ggagcggtta gcgcagaaac acggcaaggc gtctttatta cgcgccttca
     9361 tcccgctgcc gccgccgttc agcccggtac aacttattga actgcatgtt ctcaaaagca
     9421 acttctatta ccgctaccat gatgatggca gcgatgtgac ggcaacaaca gagtatcagg
     9481 gcgagatggt cgattattcg cgttacgccg tccttctcgg cagtagtgga atggcggagc
     9541 tacgctttat tcgtacccac ggcagccgtt ttactcccca ggattgcaca ctgtttaact
     9601 ggctggcacg cattatcacc ccggttctgc aatcatggct caatgatgaa gcacagcagg
     9661 tggcgctgcg tttgctggag aaagatcgcg atcatcatcg ggtactggtg gatatcacta
     9721 atgcagtgct gtcacatctt gatctcgacg atctgatcgc tgacgtcgct cgtgagatcc
     9781 atcatttttt cggtctggct tcagtcagta tggtactggg cgatcatcga aagaacgaga
     9841 agttcagcct gtggtgcagc gatctttctg cctcacattg tgcgtgtctg ccacacaata
     9901 tgcctggcga cagtgtattg ctgacacaaa cgctacaaac ccgacaaccg accttgacgc
     9961 accgtgcaga tgatctgttt ctctggcaac gcgacccgtt attactctta cttgcatcta
    10021 acggctgcga atctgcgctc cttataccgc ttacctttgg caaccataca ccgggtgcat
    10081 tgttgctggc gcatacctct tccactctct ttagtgagga aaactgccag ctactacaac
    10141 acatagccga tcgcatcgct attgccgttg gcaatgccga tgcctgccgt agaatgaccg
    10201 atttgcagga aagtttgcag caagaaaacc accagcttag cgagcagctc ctttcgaatc
    10261 tgggcgtcgg tgacattatc tatcaaagcc aggcaatgga agacctgctc cagcaggtag
    10321 atattgtggc gaagagcgac agtacggtgt tgatttgcgg tgaaaccgga accggcaaag
    10381 aggtgatcgc cagagcgatc catcaactta gcccgcgaca cgacaagccg ctggtcaaaa
    10441 tcaactgcgc tgccatcccc gccagtcttc tggaaagtga gttattcggt catgacaaag
    10501 gggcgtttac tggtgcgatt aatacccatc gtggtcgttt tgaaattgcc gatggcggca
    10561 cgttgtttct cgatgaaatt ggcgatctgc cgttagaact tcagcctaaa ctgctgcgcg
    10621 tattgcagga acgggagatt gagcgtctcg gcgggagtag aacgatcccg gtaaatgtca
    10681 gagtcattgc cgccaccaac cgtgatttgt ggcaaatggt tgaagatcgc cagtttcgca
    10741 gcgatctctt ttatcgcctg aatgtcttcc cactggaatt gcctccgctg cgcgaccgtc
    10801 cggaagatat ccctctttta gcaaagcatt tcacgcaaaa aatggcgcgc catatgaatc
    10861 gttcaattga cgccatcccg accgaggcgc tacgccagtt gatgtcgtgg gactggccgg
    10921 gcaacgtgcg cgagctggaa aacgtgattg agcgagcggt actgctgact cgcggtaaca
    10981 gtctgaattt acatcttaat gtccgacaaa gccgtttact gccgacgcta aatgaagatt
    11041 cagcgcttcg cagttcaatg gcgtagttgc tgcacccgac gacgccagag aatgacgaag
    11101 aagaacgtca gcgcattgtt caggtattgc gagaaaccaa tggcattgtt gccgggcccc
    11161 gtggcgcggc gacacgatta gggatgaagc gcaccacgct gctgtcacga atgcagcgtc
    11221 tgggaatctc ggttcgcgag gtgttgtaat ctgcttttgc aggagtatgc atgagaaaca
    11281 aactctcttt cgacttgcag ttgagcgcca gaaaagcggc aatcgctgaa cggattgccg
    11341 cccataaaat tgcccgcagt aaagtgtcgg tctttttaat ggcgatgtcc gctggcgtgt
    11401 ttatggcgat gtccgctggc gtgtttatgg cgatcggatt tactttttac ctttccgtta
    11461 tcgccgatgc cccgtcttca taggcattaa cccatctggt gggcggcctt tgctttacac
    11521 ttggctttat tttgctggcg gtttgcggca ccagcctgtt cacctcgtcg gtaatgacgg
    11581 taatggcaaa aagtcggggc gttattagtt ggcgaacttg gctgattaac gcacttctgg
    11641 tggcctgcgg taatctggca ggtattgcct gtttcagttt gttaatctgg ttttccgggc
    11701 tggtgatgag tgaaaacgcg atgtggggag tcgcgatttt acactgcgcc gagggcaaaa
    11761 tgcatcatac atttactgaa tctgtcagcc tcggcattat gtgcaatctg atggtttgcc
    11821 tggcgctgtg gatgagttat tgcggacgtt cgttatgcga caaaatcgtc gccatgattt
    11881 tgtccatcac cctgtttgtc gccagtggct ttgagcactg tatcgccaat ttgtttgtga
    11941 ttccgttcgc cattgccatt cgccatttcg cccctacctc cttctggcaa ctggcgcaca
    12001 gtagcgcaga caattttccg gcactgacgg tcagccattt tattaccgcc aatctgctcc
    12061 cggtgatgct gggtaatatt atcggcggtg cagtgctggt gagtatttgt tatcgggcta
    12121 tttatttacg tcaggaatcc tgaaaaatca gcccggcgaa acagttcgtc gggctattta
    12181 tttacgtcag gaatcctgaa aaatcagccc ggcgaaacag ttcgtcgggc tgaagattat
    12241 tcttgcgcga tttgcccatc gggccaggcg tggatcacgg ctttgatcag cgtcgccaat
    12301 ggaatggcga agaatacgcc ccagaatccc cacaaaccac cgaagatcac caccgataaa
    12361 ataatcacca gcggatgcag gttaaccgct tcggagaaca acaccggtac tagcaggttg
    12421 ccgtccagcg cctgaataat cagatacacc gcgaagcagc tccagaattc agtacctgcg
    12481 ccaaactgga atagcgccac gccaaccacc ggaatggtca ccacaaatgc gccaatgtac
    12541 ggaataagaa ccgagaagcc gaccagcact gccagcagca gcgaatagtt cagcccgaag
    12601 agcaagaacc ccagccaggt ggcgatcccc accacgatca tctccagcac tttgccgcgg
    12661 atatagttgg tgatttgttg attcatctcc ttccacacct gtcctgccag cccacggtta
    12721 cgcggcagca cccggcgaac ggcgttcagc atctgctctt tgtctttcag caggaagaag
    12781 accatcaatg gcaccagcac cagatagacg gctatggtca gcaaaccgac cagcgaggcg
    12841 agggaaattt tcaccaccga atcgcccatg gtcagcatcc gactgcgcat attttcggcc
    12901 attgcatcaa taatgcccgc gtccattaac accggatagc ggcgcggcaa cgtggcggca
    12961 aagtcagaaa gcttattgag catccccggc atatcgcgga ttaagtagat gccctgttgc
    13021 caggcgatag gcagtaccac gaacgccatc agtagcaata taccgacaaa aaccaccaat
    13081 acaatcgacg tcgcccagcg gcgggagcag ccaatagatt gcaggcgcac ggttggccat
    13141 tccagcaaat aagccagcac aatagccacc agcaacggag caagcaggcc actaaagaaa
    13201 aagataatgc caaatccggc aactaaaata accagcaagg caatcgcttc cgggtcgcta
    13261 aaacggcggc gataccattg catcaacatt tcgagcataa agaccttccc tgaacctcaa
    13321 gagcgggatt gcgatccgca attgtatcga aatgtcacaa aaaagacttc gctttttatg
    13381 acggattcag gaaactgaaa agtcatttga gtgggctaat cttcgccgtt acactcaaag
    13441 gcggcgaggt gggaacgata tttcacagta tcggtcaaat gactatcttc agaaatacag
    13501 gatagaggtt atgttcaggc agttgaaaaa aaacctggtt gcaaccctca ttgctgctat
    13561 gaccattggt caggtagccc cggcgtttgc cgacagcgca gacaccttgc cggatatggg
    13621 aacctccgca ggaagcacgc tttccattgg tcaggaaatg cagatgggcg actattatgt
    13681 ccgccagcta cgcggcagcg cgccgttaat taatgacccg ctgttaacgc aatatattaa
    13741 ttcgctgggg atgcgtctgg tttcgcatgc caattcggtt aagacaccgt ttcatttttt
    13801 tctgatcaac aacgacgaaa ttaacgcctt tgctttcttt ggcggcaacg tggtgctgca
    13861 ctctgccctg ttccgttatt ccgataacga aagtcaactg gcttcagtta tggcgcacga
    13921 aatctcccac gtcacccaac gtcacctggc gcgagcgatg gaagatcaac aacgcagcgc
    13981 gccgctgacc tgggtcggcg cgttaggttc tattttactg gcgatggcca gtccgcaggc
    14041 ggggatggcg gcgctgaccg gtacactggc gggaacgcgc caggggatga tcagtttcac
    14101 ccagcaaaat gaacaggaag cggaccgcat tggtattcag gtgctgcaac gctcgggatt
    14161 cgatccacag gcgatgccga ccttcctgga aaaattgctc gatcaggcgc gttactcctc
    14221 gcgcccgccg gaaattttat tgactcaccc gttgccggaa agtcgtctgg cagatgcccg
    14281 caaccgtgct aatcagatgc gcccgatggt ggtgcagtcg tcggaagatt tctatctggc
    14341 gaaagcgcgc acactgggga tgtataattc cggacgtaac cagctcacca gtgatttgct
    14401 ggatgaatgg gcgaaaggaa acgttcgtca gcaacgagcg gcgcaatatg gtcgtgcttt
    14461 acaggcgatg gaagccaata aatacgacga ggcgcgtaaa acgctgcaac cgttactggc
    14521 ggcagaacct ggcaacgcat ggtatctcga tctggctacc gatatcgatc ttgggcaaaa
    14581 caaagccaat gaggcaatca atcgcctgaa aaatgcccgc gatttacgca ccaatccggt
    14641 gttgcagctc aacctggcga acgcttatct gcaaggcggt caaccacaag aagcggccaa
    14701 tattctgaat cgctacacct ttaataataa agatgacagc aacggctggg atttgctggc
    14761 acaggcggaa gccgcgctaa ataaccgcga tcaggagctg gctgcgcgag cagaaggtta
    14821 tgcgctcgcc ggacgactcg atcaggccat ttcgctgttg agtagcgcca gttcgcaggt
    14881 gaaattaggc agcctgcaac aagcgcgtta cgatgcgcgc atcgaccagt tgcgccagct
    14941 gcagaaacgc tttaagcctt ataccaagat gtaattcgca ttaaaggaag aacaatgacc
    15001 aaacaggtaa aaatctacca taacccacgc tgttcaaaga gccgggaaac gctgaacctg
    15061 ctgaaagaaa acggcgtgga gccagaagtg gtgctctacc ttgagacacc cgccgatgcg
    15121 gcaacgctgc gcgatttgct gaaaatgctg gggatgaata gcgcccgtga attgatgcgc
    15181 cagaaagagg atctttataa agaactaaac ctggctgaca gttcgcttag cgaagaggcg
    15241 ctgattcagg caatggtgga taatccgaag ctgatggagc gcccgattat ggtcgcgaat
    15301 ggcaaagcgc ggattggtcg tccaccggag caggtactgg aaatcgtggg ttaagatgtg
    15361 ctccatcgct agttgaagca cattgccgga tgcgacgctt gccacgtctt atccggccta
    15421 cgaatactat ggattaactt cgcgtaggtc aggcgttcgc gccgcatccg acaataaaca
    15481 ccttatctac aacttcagaa tttctttcac aaacggaatg gtcagcttac gttgcgcggt
    15541 aatcgacgca cgatccaact gatccaacgt cataaatagc gtgcgcattt ctctgtcgag
    15601 ccgcttcagc aagaaacgcc ccacatcttc cggcagttca aaaccacgca aacgcgcgcg
    15661 taactgtagc gcctgcaact tatcttcatc agaaagtggc tgcaatttgt agatctgccc
    15721 ccagtcgaga cgcgacgcga gatccggtaa tcccagattc aactgccgcg gtggacgatc
    15781 gccggtgatc aacaaccgtg ttttgcccga ttccagaatt cgattgtaga gatcgaaaat
    15841 cgccatctcc cacaactcat cgcctgcaat acactcaatg ttgtcgatac agaccagcga
    15901 caaatgctcc ataccgtcga gcacttccgg aacaaaccag gtgcgtttat ccagcgggac
    15961 atagcccacc gcatcgccac gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct
    16021 gcgccccgcg ccttcgcgtg cccagagata gatgtaaccg ctatgttcct gacgcagcac
    16081 gttttgcagc gcggccagta aagaggagtt atcccccggc cagaaacttg caaaggtttc
    16141 gtcgtcagga agataaagtg gcaaagagag ctgtgccggt gtgttcagag atacctcaac
    16201 caggatttca caaaatcgcg agaagtttac cacagaatcc catgatgttt gaaccgggca
    16261 gcaacactgc ccggtcggta cattatttgt ctgttatatc cgcgtcttct gcgtccagca
    16321 ccacttcttc agggcgcagc acactgatca gtttgaaaat caaacttaag ccgataccga
    16381 cgatggtcgc cagcgccata ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac
    16441 tgacgccgat gatcaaaatc acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca
    16501 ctttcgattc gatcaaaaca cgaataccgg aagcaccaat gacaccataa agcagcagcg
    16561 aaacgccgcc catcaccggc aatgggatca tctggatagc ggcagccagt ttaccgacgc
    16621 aggaaagcag gatagcgaaa atcgccgccc cgccgataac ccaggtactg taaacacggg
    16681 tgatcgccat cacgccaatg ttttctccgt aagtggtatt tggcgtagag ccaaagaagc
    16741 cggagatcac ggtcgacagg ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca
    16801 gcagatcttt tttgacgata ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa
    16861 ccactaacgc cgctggcaga atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga
    16921 gggttggcag tgcaaaccag tgagcattaa taatcggcgt ggtatcgaca attcccattg
    16981 cgaaagagag cgcgtacccc accagcacgc caattaaaat cgggataatt gccaggaaac
    17041 cacgaaacag cacggaacct aaaaccgtga ccgccagggt ggtaatagag atgatgatgg
    17101 ttttggagtt tggcgtttgc ccttcagccg ggagtaaacc cgccataccg gcagctacgc
    17161 ccgccagctc cagaccgatg acggcaacga ttgcgcccat tgccgcaggt ggaaacagca
    17221 cgtccaacca gccggtcccc gctttcttca cgataaaaga aaccaggcag aacagcacgc
    17281 cgcacataat aaagccgccc agcgcgactt cataccctaa cggcaacagt aacaataccg
    17341 gtgaaataaa ggcaaagctg gaaccaagat aagccggaat tttcccttta cagatgaaga
    17401 gatacagcag cgttccaata ccgttaaata acagtacagt cgccgggtta atatgaaata
    17461 agacgggcac caggacggtt gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga
    17521 ttgtctgtaa aagtggcggt ctttcactca ccccgatagc acggcgcgtc atagtattat
    17581 cctctgtatt atgtgttata ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct
    17641 ttaattattt ttattcttta tttcgtacca aagattttgt caccggcatc gccgaggccc
    17701 ggaataatgt atccgtgctc gttcagtccc tggtcaatcg atgcagtata cagttcgacg
    17761 tccgggtgcg ctttttccag cgcagcgata ccttctggcg cagcgaccag caccagaact
    17821 ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg
    17881 gttgccagca ttgggtcaac gatcagcgcc atacgctcat cgatgttaga aaccagtttc
    17941 tggaagtacg gtaccggctc cagcgtttct tcattacggt acataccgac aacgctgatg
    18001 cgcgcgctcg gaacgttttc cagcacaccg tccatcatac caagacccgc acgcagaatt
    18061 ggcacaacgg taattttctt acctttgatc tggtcgattt ctaccgggcc gttccagcct
    18121 tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc
    18181 acttcggaag cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg catcagtccc
    18241 agcttgtgtt tgacgagtgg gtgtttgact tccacgatct tcatactctt tctcctttga
    18301 ggggcagcca caaaaaaatc gacggattat acctcctttc ttcagggcgg caatattatt
    18361 ttcgttgact ttagtcaaaa tgataacagt ttgagataaa gttattttat attcaaatgg
    18421 ttatgaaaga agattattcc atccgaaaac taacctttgc cctggcacaa gtcttctttc
    18481 gccgcgcgcc cggggaaaag acgtgcaaaa aggttgtgta aaacagtctc gcaaacgttt
    18541 gctttccctg ttagaattgc gccgaatttt atttttctac cgcaagtaac gcgtggggac
    18601 ccaagcagtg accgataaaa cctctcttag ctacaaagat gccggtgttg atattgacgc
    18661 gggtaatgct ctggttggaa gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt
    18721 gatgggcggt ctgggcggct tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc
    18781 cgtgctggtt tccggcactg acggcgtagg taccaagctg cgtctggcaa tggacttaaa
    18841 acgtcacgac accattggta ttgatctggt cgccatgtgc gttaatgacc tggtggtgca
    18901 aggtgcggaa ccgctgtttt tcctcgacta ttacgcaacc ggaaaactgg atgttgatac
    18961 cgcttcagcg gtgatcagcg gcatcgcgga aggttgtctg caatcgggct gttctctggt
    19021 gggtggcgaa acggcagaaa tgccggggat gtatcacggt gaagattacg atgtcgcggg
    19081 tttctgcgtg ggcgtggtag aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg
    19141 cgatgtgctg attgcactcg gttccagcgg tccgcactcg aacggctatt cgctggtgcg
    19201 caaaattctt gaagtcagcg gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt
    19261 agccgatcat ctgctggcac cgacccgcat ttacgtgaag tcagtgctgg agttgattga
    19321 aaaggtcgat gtgcatgcca ttgcgcacct gaccggcggc ggcttctggg aaaacattcc
    19381 gcgcgtattg ccagataata cccaggcagt gattgatgaa tcttcctggc agtggccgga
    19441 agtgttcaac tggctgcaaa cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt
    19501 caactgcggc gtcgggatga ttatcgccct gcctgctccg gaagtggaca aagccctcgc
    19561 cctgctaaat gccaacggtg aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc
    19621 cgaacaacgc gtggttatcg aataatgaat attgtggtgc ttatttccgg caacggaagt
    19681 aatttacagg caattattga cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca
    19741 gttttcagca ataaggccga cgcgttcggc cttgaacgcg cccgccaggc gggtattgca
    19801 acgcatacgc tcatcgccag cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt
    19861 catgaaatcg acatgtacgc acccgatgtg gtcgtgctgg ctggttttat gcgcattctc
    19921 agcccggcgt ttgtctccca ctatgccggg cgtttgctga acattcaccc ttctctgctg
    19981 ccgaaatatc ccggattaca cacccatcgt caggcgctgg aaaatggcga tgaagagcac
    20041 ggtacatcgg tgcatttcgt caccgatgaa ctggacggtg gcccggttat tttacaggcg
    20101 aaagtcccgg tatttgctgg tgatacggaa gatgacatca ccgcccgcgt gcaaacccag
    20161 gaacacgcta tttatccact ggtgattagc tggtttgccg atggtcgtct gaaaatgcac
    20221 gaaaacgccg cgtggctgga tggtcaacgt ctgccgccgc agggctacgc tgccgacgag
    20281 taatgccccc gtagttaaag cgccagctct gccgctggcg tttttcaatt cacctgtaaa
    20341 tcgcaagctc cagcagtttt tttccccctt ttctggcata gttggacatc tgccaatatt
    20401 gctcgccata atatccaggc agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt
    20461 caggaaaagc tatacatcga aaaagagctc agttggttat cgttcaatga acgcgtgctt
    20521 caggaagcgg cggacaaatc taacccgctg attgaaagga tgcgtttcct ggggatctat
    20581 tccaataacc ttgatgagtt ctataaagtc cgcttcgctg aactgaagcg acgcatcatt
    20641 attagcgaag aacaaggctc caacgctcat tcccgccatt tactgggcaa aattcagtcc
    20701 cgggtgctga aagccgatca ggaattcgac ggcctctaca acgagctatt gctggagatg
    20761 gcgcgcaacc agatcttcct gattaatgaa cgccagctct ccgtcaatca acaaaactgg
    20821 ctgcgtcatt attttaagca gtatctgcgt cagcacatta cgccgatttt aatcaatcct
    20881 gacactgact tagtgcagtt cctgaaagat gattacacct atctggcggt ggaaattatc
    20941 cgtagcgata gcatccgtta cgcgctgctg gagatcccat cagataaagt gccgcgcttt
    21001 gtgaatttac cgccagaagc gccgcgtcga cgcaagccga tgattcttct ggataacatt
    21061 ctgcgttact gccttgatga tattttcaaa ggcttctttg attatgacgc gctgaatgcc
    21121 tattcaatga agatgacccg cgatgccgaa tacgatttag tgcatgagat ggaagccagc
    21181 ctgatggagt tgatgtcttc cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt
    21241 gtttatcagc gcgatatgcc caatgcgctg gttgaagtgt tacgcgaaaa actgactatt
    21301 tcccgctacg actccatcgt ccccggcggt cgttatcata attttaaaga ctttattaat
    21361 ttccccaatg tcggcaaagc caatctggtg aacaaaccac tgccgcgttt acgccatatt
    21421 tggtttgata aagcccagtt ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg
    21481 ctctattatc cttatcacac ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc
    21541 gacccgagcg tactggcgat taaaattaac atttaccgcg tggcgaaaga ttcacgcatc
    21601 atcgactcga tgatccacgc cgcacataac ggtaagaaag tcactgtggt ggttgagtta
    21661 caggcgcgtt tcgacgaaga agccaacatt cactgggcga agcgcctgac cgaagcaggc
    21721 gtgcacgtta tcttctctgc gccggggctg aaaattcacg ccaaactgtt cctgatttca
    21781 cgtaaagaaa acggtgaagt ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa
    21841 aaaaccgcgc gtctttatac tgactattcg ttgctgaccg cagatgcgcg tatcaccaac
    21901 gaagtacggc gggtatttaa ctttattgaa aacccatacc gcccggtgac atttgattat
    21961 ttaatggtgt caccgcaaaa ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc
    22021 gccaacgcgc agcaagggct gcccagtggt atcaccctga agctaaataa ccttgtcgat
    22081 aaaggcctgg ttgatcgtct gtatgcggcc tccagctccg gcgtaccggt taatctgctg
    22141 gttcgcggaa tgtgttcgct gatccccaat ctggaaggca ttagcgacaa cattcgtgcc
    22201 atcagtattg ttgaccgtta ccttgaacat gaccgggttt atatttttga aaatggcggc
    22261 gataaaaagg tctacctttc ttccgccgac tggatgacgc gcaatattga ttatcgtatt
    22321 gaagtggcga caccgctgct cgatccgcgc ctgaagcagc gggtgctgga catcatcgac
    22381 atattgttca gcgatacggt caaagcacgt tatatcgata aagaactcag taatcgctac
    22441 gttccccgcg gcaatcgccg caaagtacgg gcgcagttgg cgatttacga ctacatcaaa
    22501 tcactcgaac aacctgaata accctatgcc aatacacgat aaatcccctc gtccgcagga
    22561 gtttgctgcg gtcgatcttg gttcaaacag ttttcacatg gtcatagccc gtgtggtaga
    22621 tggtgccatg cagattattg gccgcctgaa acagcgggtg catctggcgg acggcctggg
    22681 gccagataat atgttgagtg aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt
    22741 tgccgaacgg ctacaagggt tttctcctgc cagcgtctgt atagtcggca cccatacgct
    22801 gcgtcaggcg ctgaacgcca ctgactttct gaaacgcgcg gaaaaggtca ttccctaccc
    22861 gattgaaatt atttccggta atgaagaagc ccgtctgatt tttatgggcg tggaacatac
    22921 ccaaccggaa aaaggtcgca aactggttat tgatattggc ggcggatcta cggaactggt
    22981 gattggtgaa aatttcgaac ctattctcgt tgaaagccgc cggatgggtt gtgtcagctt
    23041 tgcccagctt tattttcctg gcggggtcat caataaagag aattttcagc gcgctcgcat
    23101 ggcggcagca caaaaactgg aaactttaac ctggcaattc cgtattcagg gctggaacgt
    23161 tgcaatgggc gcttccggta ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga
    23221 gaaagacggg ataattaccc cggaacgtct ggaaaaactg gtaaaagaaa ttttacgtca
    23281 ccgtaatttc gcatcgctga gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt
    23341 tcctggactg gcgattttat gtggtgtgtt tgatgcttta gccatccgtg aactgcgcct
    23401 ttctgacggg gcgttacgcg aaggcgtact gtatgaaatg gaaggacgtt tccgtcatca
    23461 ggatgtgcgt agtcgcaccg ccagcagcct cgccaaccag tatcacatag acagcgaaca
    23521 ggcccgacga gtgctggata ccactatgca aatgtacgaa cagtggcggg aacagcaacc
    23581 gaagctggcg catccgcaac tggaggcgct actgcgatgg gccgccatgc tgcatgaggt
    23641 cgggttgaat atcaaccaca gcggtttgca tcgccactcc gcttatattc tgcaaaacag
    23701 tgacttgccg ggttttaatc aggaacagca gctgatgatg gcgacactgg tgcgctatca
    23761 ccgtaaagcg attaagctcg acgatctgcc gcgctttacc ttgtttaaga agaaacagtt
    23821 cctgccactg atacagctat tgcgccttgg cgtattacta aacaatcaac gtcaggcaac
    23881 caccacaccg ccaacattga cactgattac tgatgacagt cactggacac tgcgtttccc
    23941 gcatgactgg tttagtcaga atgcgctggt actgcttgat ctggaaaagg agcaagaata
    24001 ctgggaaggc gtggctggct ggcggttgaa aattgaagaa gaaagtaccc cagaaatcgc
    24061 cgcttaatgc tttgcgggcc gacgagaaat gtcggcccgc attattcagg cactttcgcg
    24121 aatgggttcg atttcattca gcgtatcaat taacggttgc ggcttaccaa taagataacc
    24181 ctgcatataa tcgatcccca aagagagcac cgcctcgcgg atctcttcgt tttcaacgta
    24241 ctctgccact acccgcattt tcttcattcg cgccagatgg caaatcgacg ccacaatctg
    24301 ataatccaga ctatttgaca caatattgcg gataaagctg ccgtcaattt taagcagatc
    24361 ggcattcaca tttttaagcc gtgcatagct ggcgtagccg gtgccgaaat catcaatcgc
    24421 aatctggcag cctaattcct gaagatgctg caaggtgatt tgcgcctgct taacattggt
    24481 cagagcatta ctttcggtga cttcaaaaat aagttgccac gcttcaatct gatatttagc
    24541 cagcaactga ctgacttcaa caggaaaacg agcctgacat accgaggttg gagacagatt
    24601 aatagcaaaa cggtgagcgg gcatcttcgc tctgttttca gccataaatt gcagcgtatg
    24661 ctcaatgacc cacatgtcga tactcgacga taaaccaaat tcgtgcgcga ccggcaagaa
    24721 gctaccgggg ccgatcagtt catcattctc acctttcatg cgcagaagaa tttcatggta
    24781 aacatcacca cgcataccgg taatcggctg ggccatcagg aaaaaatggt tgtgttccag
    24841 cgcctgctgt agtcgattca tcatcgcgac tttatctttc aattcgcgtt gcaaatacat
    24901 tgccccgcaa cgctgcatat tttccggggc gttggtcacg atggaaagtt cggcgaccgt
    24961 atttagctct cccagcagca ggtagatatg attcactggc gagcgcacat agcagtaact
    25021 gacgccaatc tgcggttgca tcggcatccc atcccaaaag aaacgaaatt gcttgagatg
    25081 actatccagt gcggtaatgc gctcctggtg cgattctgta ttcagtcgca gcgcgagatc
    25141 gttacccgaa agctgataaa catcttcacc cggttccagc aagggtgaca gccagtgaga
    25201 aagtttttgc tt
//
LOCUS       DAJTMX010000053        24654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTMX010000053 DAJTMX010000000
VERSION     DAJTMX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.053"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..386)
                     /locus_tag="OO145_002117"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO145_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO145_002118"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO145_002118"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5467229.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO145_002119"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO145_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5467230.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO145_002120"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO145_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5467231.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO145_002121"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO145_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5467232.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO145_002122"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO145_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5467233.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO145_002123"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO145_002123"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5467234.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO145_002124"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO145_002124"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5467235.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO145_002125"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO145_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5467236.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO145_002126"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO145_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5467237.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO145_002127"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO145_002127"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5467238.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO145_002128"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO145_002128"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5467239.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO145_002129"
     CDS             10748..11212
                     /locus_tag="OO145_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5467240.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO145_002130"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO145_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5467241.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO145_002131"
     CDS             12236..13672
                     /locus_tag="OO145_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5467242.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO145_002132"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO145_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5467243.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO145_002133"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO145_002133"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5467244.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO145_002134"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO145_002134"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5467245.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO145_002135"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO145_002135"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5467246.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO145_002136"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO145_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5467247.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO145_002137"
     CDS             complement(20505..20798)
                     /locus_tag="OO145_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5467248.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO145_002138"
     CDS             complement(20795..22084)
                     /locus_tag="OO145_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5467249.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO145_002139"
     CDS             complement(22140..23078)
                     /locus_tag="OO145_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5467250.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDTTAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO145_002140"
     CDS             complement(23098..23862)
                     /locus_tag="OO145_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5467251.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24182..>24654
                     /locus_tag="OO145_002141"
     CDS             24182..>24654
                     /locus_tag="OO145_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5467252.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5949 c         6484 g         6118 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg tatcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcggtggggg ggggcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    24001 gcgattgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    24061 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    24121 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    24181 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24241 acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca
    24301 tgacaatgaa accgagctga tttacgtcaa gaaaggggtt gcaaggttta ccatcgattc
    24361 ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca ggctgcatgc
    24421 ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca
    24481 gtttcagggg gctgaggaaa atcagttact gcaaccgcat tcttgtccgg taattgccgc
    24541 ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga ttttgccgca
    24601 aagtaaaaat agccaaacat cttcgttatg ggatgcatta gcctatacat tagc
//
LOCUS       DAJTMX010000054        24576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTMX010000054 DAJTMX010000000
VERSION     DAJTMX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.054"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            94..1209
                     /gene="adrA"
                     /locus_tag="OO145_002142"
                     /gene_synonym="dgcC"
     CDS             94..1209
                     /gene="adrA"
                     /locus_tag="OO145_002142"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5467253.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1226..2035)
                     /gene="proC"
                     /locus_tag="OO145_002143"
     CDS             complement(1226..2035)
                     /gene="proC"
                     /locus_tag="OO145_002143"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5467254.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2155..2613
                     /locus_tag="OO145_002144"
     CDS             2155..2613
                     /locus_tag="OO145_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5467255.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2796..3320
                     /gene="aroL"
                     /locus_tag="OO145_002145"
     CDS             2796..3320
                     /gene="aroL"
                     /locus_tag="OO145_002145"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5467256.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            3370..3561
                     /gene="yaiA"
                     /locus_tag="OO145_002146"
     CDS             3370..3561
                     /gene="yaiA"
                     /locus_tag="OO145_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5467257.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3819..4496
                     /locus_tag="OO145_002147"
     CDS             3819..4496
                     /locus_tag="OO145_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5467258.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4568..4852
                     /gene="ppnP"
                     /locus_tag="OO145_002148"
     CDS             4568..4852
                     /gene="ppnP"
                     /locus_tag="OO145_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5467259.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            5007..5342
                     /locus_tag="OO145_002149"
                     /pseudo
     CDS             5007..5342
                     /locus_tag="OO145_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            5339..5794
                     /locus_tag="OO145_002150"
     CDS             5339..5794
                     /locus_tag="OO145_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467260.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(5872..6783)
                     /gene="rdgC"
                     /locus_tag="OO145_002151"
     CDS             complement(5872..6783)
                     /gene="rdgC"
                     /locus_tag="OO145_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5467261.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            6908..7816
                     /gene="mak"
                     /locus_tag="OO145_002152"
     CDS             6908..7816
                     /gene="mak"
                     /locus_tag="OO145_002152"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5467262.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(7959..9140)
                     /gene="araJ"
                     /locus_tag="OO145_002153"
     CDS             complement(7959..9140)
                     /gene="araJ"
                     /locus_tag="OO145_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5467263.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(9266..12409)
                     /gene="sbcC"
                     /locus_tag="OO145_002154"
     CDS             complement(9266..12409)
                     /gene="sbcC"
                     /locus_tag="OO145_002154"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5467264.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(12406..13608)
                     /gene="sbcD"
                     /locus_tag="OO145_002155"
     CDS             complement(12406..13608)
                     /gene="sbcD"
                     /locus_tag="OO145_002155"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5467265.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            13798..14487
                     /gene="phoB"
                     /locus_tag="OO145_002156"
     CDS             13798..14487
                     /gene="phoB"
                     /locus_tag="OO145_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5467266.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KQLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            14545..15840
                     /gene="phoR"
                     /locus_tag="OO145_002157"
     CDS             14545..15840
                     /gene="phoR"
                     /locus_tag="OO145_002157"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5467267.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            16247..17566
                     /gene="brnQ"
                     /locus_tag="OO145_002158"
     CDS             16247..17566
                     /gene="brnQ"
                     /locus_tag="OO145_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5467268.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            17642..19015
                     /gene="proY"
                     /locus_tag="OO145_002159"
     CDS             17642..19015
                     /gene="proY"
                     /locus_tag="OO145_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5467269.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            19171..20988
                     /gene="malZ"
                     /locus_tag="OO145_002160"
     CDS             19171..20988
                     /gene="malZ"
                     /locus_tag="OO145_002160"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5467270.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(20993..21566)
                     /gene="acpH"
                     /locus_tag="OO145_002161"
                     /pseudo
     CDS             complement(20993..21566)
                     /gene="acpH"
                     /locus_tag="OO145_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            21659..22729
                     /gene="queA"
                     /locus_tag="OO145_002162"
     CDS             21659..22729
                     /gene="queA"
                     /locus_tag="OO145_002162"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5467271.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22784..23911
                     /gene="tgt"
                     /locus_tag="OO145_002163"
     CDS             22784..23911
                     /gene="tgt"
                     /locus_tag="OO145_002163"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5467272.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            23934..24266
                     /gene="yajC"
                     /locus_tag="OO145_002164"
     CDS             23934..24266
                     /gene="yajC"
                     /locus_tag="OO145_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5467273.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            24294..>24576
                     /gene="secD"
                     /locus_tag="OO145_002165"
     CDS             24294..>24576
                     /gene="secD"
                     /locus_tag="OO145_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5467274.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGV"
BASE COUNT         5630 a         6102 c         6639 g         6205 t
ORIGIN      
        1 agtgctaacg ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc
       61 cccgtctcta taatttggga aaattgtttc tgaatgttcc caaaaataat gaatgatgaa
      121 aactttttca aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa
      181 catcagcggt ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg
      241 gctggcatgt tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg
      301 tggctggtgt tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc
      361 agggccgtcg atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg
      421 ggaatgtggg taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt
      481 ctgtgtctga atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg
      541 atggtggttt cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt
      601 gctccgctgg aatggtggct ctcccttccc attattgtcg tttatcctct gatgtttggc
      661 tgggtcagct accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg
      721 agtactcgcg acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc
      781 aatgaatttg ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatcgatatc
      841 gaccatttca agagcatcaa cgatacctgg ggtcatgatg tgggcgatga agcgattgtg
      901 gcgcttaccc gacagttaca aattaccctg cgcggtagcg atgtgattgg tcggtttggc
      961 ggcgatgagt ttgcagtaat catgtccggt acgccagctg agagcgccat taccgccatg
     1021 ttacgggtgc gtgaagggct aaacacgtta cgcttgccga atacgccaca ggtaacttta
     1081 cggattagtg tgggggttgc gccgctgaac ccacaaatga gtcactatcg tgagtggttg
     1141 aaatcggcag atttggcgct ttacaaagca aagaaagccg gacgtaaccg caccgaagtg
     1201 gcggcctgac gtccggcgaa agtcatcagg atttgctgag tttttctgat ttttccatac
     1261 acttcgtcat cgcttcgatc actgcggcac ggaagccttt ctcttccagt acgcgtaccg
     1321 cttcaatggt ggtgcctccc ggtgagcaga ccatatcttt cagtgccccc ggatgttctc
     1381 ccgtttccag caccattttt gcggaaccca ttaccgcctg agcggcaaat ttatacgcct
     1441 gggcgcgtgg catcccgccc agcacggcgg cgtcggccat cgcttcgata aacataaata
     1501 cgtaggctgg cgaagaaccg ctcacaccga ccaccgggtg gatcatcggc tcagcaatta
     1561 cttccgcttc gccaaagcag cggaaaatat tcagcacatc agcggtatct tctggggtta
     1621 ccagcgcgtt tggcgttacg gaggtcatcc cggcattaac cagcgcggga gtgttcggca
     1681 tggcgcggat aattttccgg tcatggccca gcgcgcgggc aagctggtcg agcgtgacac
     1741 ctgcagcaat agaaacgacc agagagtctt tattcaggct ggaggtgatt tcgctaagca
     1801 ctttaatcat gatgccaggt ttaacggcag caaaaatgat gtcggcgatt tgcgccactt
     1861 cttgcgccga ttctgcggcg ttgatgccga actggtcatg cagggcggcg actttatccg
     1921 gggagggggt gtatacccag atttgccctg gaagcacctg accgctggca atcagaccgc
     1981 cgagaatggc ttttcccata ttgccgcagc caataaaacc gattttcttt tccattgcct
     2041 cactcctgcc gtgaaattca ttgttttgat aatcgctggc agaagcataa acagaactat
     2101 gccggaaggc aaaagcgcga cacaatagag gattacctaa caaaggatga ctttatgaca
     2161 atttgggtgg atgccgacgc gtgtcccaat gtaattaaag agattttgta tcgcgcggcg
     2221 gaacgtatgc agatgccgct ggtactggta gcaaaccaga gtttacgcgt gccgccatcg
     2281 cgatttattc gtacgctgcg cgtcgcggca ggtttcgacg ttgccgataa cgaaattgtc
     2341 cggcagtgtg aagcgggcga tttggtgatc accgcagata tacctttggc tgctgaagcc
     2401 atcgagaaag gcgcagcggc gcttaattcg cgcggcgaac gttacacgcc agcgaccatt
     2461 cgtgagcgcc tgacgatgcg cgattttatg gataccttac gtgccagtgg gatccagacc
     2521 ggcggaccag atagcctttc acaacgtgac cgccaggcct ttgccgcgga gctggagaag
     2581 tggtggctgg aagtgcaacg tagtcgtggc taaatgtaat ttattattta cacttcattc
     2641 ttgaatattt attggtatag taagggatgt attgagattt ttactttaag tggaattttt
     2701 tctttacaat cgaaattgtg ctagtttgat ggtatgatcg ctattctcat gacaccggct
     2761 ttcgccgcat tgcgacctat tggggaaaac ccacgatgac acaacctctt tttctgatcg
     2821 ggcctcgggg ctgtggtaaa acaacggtcg gaatggccct tgccgattcg cttaaccgtc
     2881 ggtttgtcga taccgatcag tggttgcaat cacagctcaa tatgacggtc gcggagatcg
     2941 tcgaaaggga agagtgggcg ggatttcgcg ccagagaaac ggcggcgctg gaagcggtaa
     3001 ctgcggcatc caccgttatc gctacaggcg gcggcattat tctgacggaa tttaatcgtc
     3061 acttcatgca aaataacggg atcgtggttt atttgtgtgc gccagtatca gtcctggtta
     3121 accgactgca agctgcaccg gaagaagatt tacggccaac cttaacggga aaaccgctga
     3181 gcgaagaagt tcaggaagtg ctggaagaac gcgatgcgct atatcgcgaa gttgcgcata
     3241 ttatcatcga cgcaacaaac gaacccagcc aggtgatttc tgaaattcgt agcgccctgg
     3301 cacagacgat caattgttga ttttagagcg cctatactta acgttcatcc cgtgaaataa
     3361 ggaagaacga tgccaacgaa accgccttat cctcgtgaag catatatagt gacgattgaa
     3421 aaaggaaagc caggacagac ggtaacctgg taccaactca gagccgatca tcctaaacca
     3481 gactcgttga tcagtgaaca tccgacggct caggaagcga tggatgcgaa aaaacgctat
     3541 gaggaccctg acaaagagtg accgcatcag actgctcgga agggattctg agtgccacta
     3601 caagggatct gcgtcacatt tttcataatt tatgtttttc taataattag aatattaaac
     3661 aataacattc cattactgga atcatttgga atctttacat tatgccgtgc acgtctgctg
     3721 ctacgctttt tgtcatttgt agcacaagta agtgtcagca gtggtgcttc acacttgccc
     3781 ggtaattaac gacgaaagaa aagtaaggtg gatgaacaat gagtgcgtcg ttggcgatcc
     3841 tgaccatcgg cattgtacct atgcaggagg ttttgccgct cctgacggaa tacattgacg
     3901 aagataatat ttcccatcat agcctgctgg ggaagttaag tcgtgaagaa gtgatggcgg
     3961 agtacgcgcc agaagcaggc gaagacacca ttctcacatt attaaatgac aaccagctgg
     4021 cccatgtttc gcgtcgtaaa gtggagcgag atctgcaagg tgtggttgaa gtgctcgata
     4081 atcagggtta tgacgtcatt ttattaatga gtacagcaaa cattagtagt atgactgcgc
     4141 gtaatacgat ctttcttgag ccgtcgcgaa tattgcctcc actggtttcc tctattgttg
     4201 aagatcatca ggtgggggtt atcgttccgg ttgaggagat gctgcccgtt caggcgcaaa
     4261 aatggcaaat tttgcagaaa tcgccggtat tttcattggg taaccccatt catgattcag
     4321 aacaaaaaat cattgatgct gggaaagaat tactggcaaa aggggctgat gtcatcatgc
     4381 tggattgttt aggatttcac caacgtcatc gcgatttact gcaaaaacag ctcgatgttc
     4441 ctgtcttgct ctctaacgta ttgattgcac ggctggctgc ggaattactg gtgtaatttt
     4501 gcgtgacagc cagcgcctct ggcccctata gtgaagtaga tgttcaacta ccagacaggg
     4561 ccagtttatg cttcaaagta atgagtactt ttccggcaaa gtgaaatcga tcggcttttc
     4621 cagcagcagc actggtcgcg ccagcgtggg tgttatggtt gaaggcgaat acaccttcag
     4681 caccgctgag ccggaagaga tgacggtaat cagtggcgcg ctgaatgtgt tactgcctga
     4741 cgcgaccgac tggcaagtgt atgaagccgg ttcggtgttt aatgttcccg gtcacagtga
     4801 gtttcatctt caagttgccg aacctacctc ttatctgtgc cgctatctgt aattcctcgc
     4861 cttccccttg aacgggaggg catttttctg aaatatcctc tctttaaccc ataataatat
     4921 ttcctttgct gcgatttttt caatttccga tatattcata attcatcaag gttgatataa
     4981 atatcagtga agatctccag gtattttttg cggaactggc tacgataaaa gataaatcag
     5041 atgatgaatg gtggcgtgca ttgctgcaaa gtcgcaatga tcatttgcgc caaacggcat
     5101 tacgcaacgc acataccccg gcgtcgttgt taacgacatt gactgagcct caggatcggt
     5161 cgctggctat taataatcca cagctggctg ccgatgtgaa aacggcgtgg ttaaaagagg
     5221 atccatcatt actcttattt gtcgaacaac ccgatctttc gcagttacgt gatttagtga
     5281 aaaccggggc aacgcggaaa attcgcagtg aagcgcgtca ccggcttgag gaaaaacaat
     5341 gacacaacgt ccatggagca agttacagcg ggagatctat gacttactga cgccaacgat
     5401 caacctgcaa attcactgta cccgttatcc catgcgcagc caaaatggtg gaagtaccga
     5461 tttaccgcgt tattggatca cgttggataa aaacgtgatc tgggattacc cgaaagactt
     5521 tattgcaggt aacgctggag tgcgtaattt tcacggtgaa acttgctggt atccttacct
     5581 gacggatatt tgttcaattt ctgatttatt gcgtgaatat attgataccc caaaagcgga
     5641 gctgttaaca aaacaattta ccagcgataa atgggggtta gtgaatattc tgcgtgctgc
     5701 tgacaggcga atagggatga ggcgattaga tcagttacgg cgtaaaacgc ataatattgc
     5761 tgcgctaaaa attattgctc gccgtagcga ataattatat gcctggtgtg gcttcgtacg
     5821 ccggataaga cgcggcaggc gtcgcatccg gcattaaagg aaaatcagca attaacgttg
     5881 tgcttcgcca cctaatcctt caatcaggtt ttgaattaac gctgccagtt caccagtcat
     5941 caggatgaaa tcggcatcaa aacgctgggc gaaatcttca cggtcgatat cttcgttttg
     6001 atcgcgcagc tcgtcgcaga acttaagacg cttgagcgaa ccatcgtcgc acatcacaaa
     6061 ctgaatgcgc tgctgccagt cgagcgccag tttagtcacc acttttccgg cttcaatgtg
     6121 attggtgatc tcttcgctgg tcagatcttg tttcttcgcg cggatcacgc cgccatcttc
     6181 cagcaacgat ttcagctcgg cttcatcaag cagctggaag ccctgtgccg cactaccgga
     6241 gcgaacccat tcggtcagcg tcagttcaat cgggttttcc atgctcaatg gtacaaccgg
     6301 taacgacccc aggcttttac gcagtaatgc cagcgtatct tccgcttttt tggcactggc
     6361 gcagtccacc ataatcaaac cgttaaccgt gtcgagccac atcattgtct ggctaaaacg
     6421 gctgaaagca cgcggcagca gagagtgcag cacttcgtct ttcagcgaat ctttttcggt
     6481 tttcttcagc ttacgcgcct gttccgcttc cagtttggcg attttcgctt ccagcgcctg
     6541 tttaatcacc ggagacggga ggattttttc ttctttgcgc gcgcagataa caatttgacc
     6601 attggcaacg tgcgttaacg catcgctgtg cgatcccatc ggaggaaccc agcccatctt
     6661 cgccatgtcc tggctgccgc atggggtaaa tgccatcgag gctagctgtt tttccatctc
     6721 ttctgcacgc agcgaaatct cgcggctaag acggtaaacc attaaatttt tgaaccacag
     6781 catgataatt tccacggcct tgtcgttaaa tttagcgggc atgataacga attgtcggcg
     6841 gccttgcatt gccaatccgg ttgtccgtct ctacgctatt gatattgaaa aaaataagga
     6901 gagtacagtg cgtataggta tcgatttagg cggcaccaaa actgaagtga ttgcactggg
     6961 cgatgcaggg gagcagttgt accgccatcg tctgcccacg ccgcgtgatg attaccggca
     7021 gactattgaa acgatcgcca cgttggttga tatggcggag caggcgacag ggcagcgcgg
     7081 aacggtaggt atgggcattc caggttcgat ttcgccctac accggtgtgg tgaagaatgt
     7141 caattcaacc tggctcaacg gtcagccatt cgataaagac ttaagcgcga ggttgcagcg
     7201 ggaagtgcgg ctggcaaatg atgccaactg tctggcggtt tcagaagcag tagatggcgc
     7261 ggcagcggga gcgcagacgg tatttgccgt gattatcggc acgggatgcg gcgcgggcgt
     7321 ggcattcaat gggcggacgc atatcggcgg caatggcacg gcaggtgagt ggggacacaa
     7381 tccgctaccg tggatggacg aagacgaact gcgttatcgc gaggaagtcc cttgttattg
     7441 cggtaaacaa ggttgtattg aaacctttat ttcgggcacg ggattcgcga cggattatcg
     7501 tcgtttgagc ggacatgcgc tgaaaggcag tgaaattatc cgcctggttg aagaaagcga
     7561 tccggtagcg gaactggcat tgcgtcgcta cgagctgcgg ctggcaaaat cgctggcaca
     7621 tgtcgtgaat attctcgatc cggatgtgat tgtcctgggg ggcgggatga gcaatgtaga
     7681 ccgtttatat caaacggttg ggcagttgat taaacaattt gtcttcggcg gcgaatgtga
     7741 aacgccggtg agtaaggcga agcacggtga ttccagcggc gtacgcggcg ctgcgtggtt
     7801 atggccacaa gagtaaaaaa cgtaggcaat tggcgcatca tgcctgatgc gacgcttgcc
     7861 gcgtcttatc aggcctacaa aatgtgccag aaccgcgtag ggcggataag gcgttcacgc
     7921 cgcatccggc aataagcaac gcgcaccgtg acagtaatct accccagtgg tttcgccagc
     7981 accggactat ccgccgcttg ctggcgctta tagcgaccat acagcagcaa cgatgacatc
     8041 gcagcaaacg aaagcagggc ggcaggcagc gccacgtaat tatatgccag ccccagcgtc
     8101 agcatcatac ctccgcaata tgcgccgacg gcgctaccga ggttaaacgc tatttgccca
     8161 cctgcggcac ctaataactc tcctcctttg gcattttgca gtaacaatat ttgcagaggt
     8221 gctgaaaggg caaataatcc cgcgcaacaa ataaaagcaa aaataagcga cgttgttttc
     8281 atgccgccgc agaaaaagag catcagcagt gccagtacaa tgataaagtc agtcactgct
     8341 gcaatgcgca gtggtgaata acgtcctgaa atcctgccac ttagcatatt tcccagcacc
     8401 atccctagcc caactaacat cataataaag gtcatcgccg tttccgaaaa accggaaata
     8461 aacatcatgt atggctttac gtagctgaac caggcaaaca cacctgcgtt gccaaacatc
     8521 gtggcggcga aaattaacca cggggccggg ctgcgcaaaa agtgaaattg ttcgcgcaga
     8581 tttcctttcg cctcgtcgcg aatatctggc acccaaaaat agaccgatgc catcaccgca
     8641 atattaaaaa cagcgatcaa taaaaaggtg taacgccagc attcctgact taaatacgtt
     8701 cccagcggaa tgcccagcaa attggcgact gtcatcccgg aaaccatccc cgccacggcg
     8761 gcggtgactt ttccgggttt gataattttt gataacacga tcgctccgac gccaaaaaat
     8821 gcgccatgcg gaaagccgga taccagccga ccaatggcga gcatcaggta agacgaagag
     8881 agcgtgaaca tggcgttgcc aatgacgcac aacgccacca gaaacaacaa gatatgtttg
     8941 agtgagtagc ggctggaaaa gagtgcgatg attggcgcac cgaccaccac caccagtgca
     9001 taatacgaga tcatatgccc ggcagcagga atcgaaattc ctacgttatg cgccagctcc
     9061 gtgagcacgc tcataatgcc aaattcggcc atccccaaac caaacgtgcc cagagccaaa
     9121 gataaaatga cttttttcat accaccatca caaccaccag aacggctacc aatcaccgta
     9181 gccaataaag tgatagcttg caggataacg accagcaagg ccaagagata attcacccct
     9241 ctgtattcat tatcctgctg aatagttatt tcactgcaaa cgtactttcc agtttgctgt
     9301 agcccaggcc gttgatcttt ttcactttga tctgcaccgg aatacgctct ttcatcgctt
     9361 ctacgtggct aatcacaccg atggttttgc cactggcgtt cagggcatcc agcgcatcaa
     9421 gggcggtatc cagcgtttcg ctatccagcg tgccaaaacc ttcatcaagg aacagcgagt
     9481 caatacgtgt tttatggctg accagatccg aaagcgccag cgccagcgcc agactaacga
     9541 ggaaactttc gccgccggaa agggtacggg tatcgcgtac cgcatctgcc tgccaggtat
     9601 caacaacctc gacttccagc gcctcgctgg ctttgcgctg taacagatag cgcacgtgca
     9661 gccgggtaag ttgctgatta gcgagatgga ctaaattatc cagcgtcagc ccctgggcaa
     9721 acttgcggaa tttatcgccc tctttggaac ctattagcga attcagatat ccccagtcct
     9781 caacctgctg cgtcatttga gcaatttgct gcattaaggt ttgttgttgc tgacggttat
     9841 ctgcatcctg cttcagctgc tggcgaatct cgccttgact cgtggtgttt tcacgcaact
     9901 tttggtgagt ttgcgctaac tcttgctgaa tctgctccac cgtcacagtg agagccaacc
     9961 cgtcaggtcg gtgttgttga tgctgtgcca gcgtttctgc tgtctgagtg accagcgttt
    10021 gcgcctgacg gcgctggttt tccagattct gcttgagctg ttccagctgc gttagtgttt
    10081 gttcatccat tagcgccgca aggaacgcct gctgatcgtc aaagacgctg gcctgtagcg
    10141 cggtgtcaaa ctgcgcttgg gctttttgca gactttgcgc cgccagaaca tcctgttgct
    10201 gtaacgtctg ctgctggctg tgtaatgcga gacattgttc atgaacctgc cgccagttat
    10261 ccagcgccac agtttcttcg ctgtgcggga gatcatcact ttgcggcaac gtttccagaa
    10321 tcggtgtcag ctgctgaata cggttttgca gcgcggttaa ttcgttctgg cgttgctgcc
    10381 agctctgcgc ttcttgctga cgtgtcgcca accagctctc ttcttcatct tcctgtggca
    10441 atgtcagtgc ataacccgcc aatgccgtta agagctgctg ctgacgttgt tcaatttgct
    10501 gttgatactg gataatttgc tgattatgcg cggcaatctg cccttgtaat tcatgtcgtt
    10561 ggctgagtaa ccgcagctgg cgttcgtgct catcttgtgc atccagccac ggttgaatat
    10621 cgtcctgtgg ctgcaaggtg atattgaggc tggccgtgac ggcttgccat tgttgagtaa
    10681 gtgcttgctc atcttgtcgg aggctttgcg cttcgttttc atcgcgctga agctgcttcg
    10741 ttaatgcatc cagttgcccg cgcagcgccg caccttcttc gccgagcttt ttaacttcgt
    10801 tttccagcgc cagtaatcga gactgattaa caccaggctc cagcgcctga tacgcctcga
    10861 ccgccgggtg gctggtggaa ccacaaagtg gacaaggctg acccgcctgt aactgtgcac
    10921 gctgggcttc cagcgttttg atgcgcgctt cctgctcgca aatggttttc acatcggcaa
    10981 gttgctgcgt cttttcttta taacgctggc gcatttcgtt aagtgcgaca ttacgttgcg
    11041 tctgttccag agtgacattc tggatagtga ccattaactg cgccagacgt ttttgttggg
    11101 gaacaatctg cccatgcagc gcgaccaggc gctgacgcag tgggcgttgc tcggcatgtt
    11161 gcgccagggc ggtagcaact tcatcggcgg ttaacgtcaa cgtgatcgcc gcaagcgcat
    11221 taagtttttg ctcagcatgg gttaactgtt gctgccattg ccgcagatgc tcgcgatcgc
    11281 tggtttgttg ggagaactgc gcacgccaac ccgccagttc gttgttccac tgacggaagc
    11341 ggtcgtgttc ctgtaaccag gtattcaggc tttgttgctg ctgctgtaat tctgctgact
    11401 gcttcgccgc gtggtggcga atgctcgcgc gaagcgccat tgtgctctgt aagcgagtat
    11461 ttacttcttc aatctgctgg cgaatatgcg ccagcgccgc gctgtgttct gcgatgcgtt
    11521 cccagtgtgg acgaagattt cgtgccggtt gtgccagact aagcgccgcc agttgaggtt
    11581 gcgctttttc ttcttcggct aacgcctgtt gcaaggcctg ttgacggcgg ctggcttctt
    11641 gctgcaattc gtcctgacgc gttaaccagt ttagcgattg ttgttcttgc tgctgcgcgg
    11701 taattaactg tttttcttcg tcagtaagta cctgcaaact cgctgtcagc gattgcactt
    11761 gttccggcgt gagcaacgtg acgccgctgg cctgcgcttg cagcttctcc agctcagtgc
    11821 gggccgattt gtgctgctca aaaaccatcg ccgagatttg cccgtagatt tcagtgccgg
    11881 ttaactcctc gagcaattcc gcgcgttctt tgggtttggc attcaggaag gcagcaaatt
    11941 gcccctgcga aagcagcatc gaacgggtga agcgcccgta atccagcccg gttaacgtcg
    12001 ctgtcagttc cagcttatct ttcactttgt cggcgagaat tttgccgtcg gcgcagcgcg
    12061 ccagctctac gcgaggcacc tgcaaattac cgtccggctg gttacgcgcc cgattctggc
    12121 tccagaacgc gcgatacgct tcacctttca cttcaaactc cacctccgcc agacattcag
    12181 cggtatcgcg ggtcatgaga tcattttgcg attgtgaaac gttagagaga cgcggagttt
    12241 cgtgatacag cgccagacaa atggcgtcca gcagggtggt tttccccgca ccggttgggc
    12301 cggtaatggc aaacagcccg ttgctggcga acggctcgcg ggtgaaatca atcttccatt
    12361 cgccttttaa tgagttcagg tttttcaggc gcaggctgag aattttcatg cttcgtgttc
    12421 tccggcgagg gtacgcaacg tcgtggtgaa aagatgctgc agacgttgct gctgcgattc
    12481 atccaggtct tccagtgcca gacggcgatt gaacacctct tcgacgctga gttcgctgag
    12541 ggtttcacgc tgttggctgg ctaacacacg ctcgcgctgt tcacgactcc gacgtaccag
    12601 caatacttcg acaggcaatg attcggttaa tgcctggatt ttgcgctgaa tatcatgcag
    12661 atactcatca gtagtgattt cgatatccag ccagacaggt ggctcctgcg atacatcgcg
    12721 ccactgttcc agctgtgcgg taatcgacgc cagatcgcct ttcagcaccg ccatgggttg
    12781 agttaccggt acgttcaggt tttccacgct ctctaatttg ccgtttgaaa atgtcaccag
    12841 atggacatat ttactcttac cgcattcatc aaaactcagt ggaatggggg aaccgcaata
    12901 gcgaacatgt tccatgccgc caataatctg tgcgcggtga atatgcccga gcgcgatgta
    12961 gtcggctggt ggaaagtttt gtgccggaaa cgcgtccagc gtgccaatat aaatgtcacg
    13021 cacggcgtca cttttactgg aacccacggt cgttaaatgt cccgtggcga tgatgggcag
    13081 aggctgatcg ccgcgcagtt tgcaggcatc ggcatagtgt tgttggtaat aatcggtaat
    13141 cgctgccagt aaatgctgct gtttttcaat accgttaagc cccgcctggc tggaaataat
    13201 gtcacgcggg cgtaaaaacg gaatggggca cagcactgcg cctggcgtcc cgtcgcgacg
    13261 aggcaagatt tgcggcgcat gtccggcgct ggcgaccacg gttgtattga ggaacgccat
    13321 gatatcgcgc gattcattca gcgtggcgac cgagtcatgg tttcctgcca gtaccaccag
    13381 atgacagcca gtttgctgta aattgacaac aaaacggttg tataacgtgc gggcgtaact
    13441 gggcggcgag ccggtatcga aaacatcacc agcaacaata atcgcatcca cctgatgggt
    13501 ttgtgctgtc tccagcagcc agtcaagaaa agcctgatgt tcagcttcgc ggcttttact
    13561 gtagaagttc tggccgagat gccagtctga ggtgtgaagg atgcgcataa cggttccctg
    13621 gcgaaaaagc atggtcgcga ttatacccaa acagatgtgc catttgcttt tttctgcgcc
    13681 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
    13741 gacgcataat gacgtcgcat taatgatcgc aacctattta ttacaacagg gcaaatcatg
    13801 gcgagacgta ttctggtcgt agaagatgaa gctccaattc gcgaaatggt ctgcttcgtg
    13861 ctcgaacaaa atggctttca gccggtcgaa gcggaagatt atgacagtgc tgtgaatcaa
    13921 ctgaatgaac cctggccgga tttaattctc cttgactgga tgttacctgg cggctccggt
    13981 atccagttca tcaaacacct caagcgcgag tcgatgaccc gggatattcc agtggtgatg
    14041 ttgaccgcca gaggggaaga agaagatcgc gtgcgcggcc ttgaaaccgg cgcggatgac
    14101 tatatcacca agccattttc gccgaaggag ctggtggcgc gaatcaaagc ggtaatgcgc
    14161 cgtatttcgc caatggcggt ggaagaggtg attaagatgc agggattaag tctcaacccg
    14221 acatctcacc gagtgatggc gggcgaagag ccgctggaga tggggccgac agaatttaaa
    14281 cagctgcact tttttatgac gcatcctgag cgcgtgtaca gccgcgagca gctgttaaac
    14341 cacgtctggg gaactaacgt gtatgtggaa gaccgcacgg tcgatgtcca cattcgtcgc
    14401 ctgcgtaaag cactggagcc cggcgggcat gaccgcatgg tgcagaccgt gcgcggtaca
    14461 ggatatcgtt tttcaacccg cttttaacgc cttgctcatc ggacgcagag cagggcttat
    14521 gatttcttaa ctggagtatc ttacgtgctg gaacggctgt cgtggaaaag gctggtgctg
    14581 gagctgctac tttgctgcct cccggctttc atcctgggtg cattttttgg ttacctgccc
    14641 tggtttttgc tggcatcggt aacaggactg cttatctggc atttctggaa tttattgcgc
    14701 ctttcatggt ggctgtgggt ggatcgcagt atgaccccgc caccggggcg tggtagctgg
    14761 gaaccgctac tatacggctt acaccagatg cagctgcgaa ataaaaaacg ccgccgtgaa
    14821 ctgggcaatc tgattaaacg ctttcgtagc ggcgcggagt cgctgcccga cgcggtggtg
    14881 ctgaccacgg aagagggcgg tattttctgg tgtaacggtc tggcgcaaca aattcttggt
    14941 ttgcgctggc cggaagataa cgggcagaac atccttaacc tactgcgtta cccggagttt
    15001 acgcaatatc tgaaaacgcg tgatttttct cgcccgctca atctggtgct caacaccggg
    15061 cggcatctgg aaattcgcgt catgccttat acccacaaac agttgctgat ggtggcgcgt
    15121 gatgtcacgc aaatgcatca actggaaggg gcgcggcgta acttttttgc caacgtgagc
    15181 catgagttac gtacgccatt gaccgtgtta cagggttacc tggagatgat ggatgagcag
    15241 ccgctggaag gcgcggtacg cgaaaaagcg ttgcacacca tgcgcgagca gacccagcgg
    15301 atggaagggc tggtgaagca gttgctaacg ctgtcgaaaa ttgaagccgc gccgacgcaa
    15361 ttgctcaatg aaaaggttga tgtgccgatg atgctgcgcg ttgttgagcg cgaggctcag
    15421 actctgagtc agaaaaaaca gacatttacc tttgagatag ataacggcct caaggtgtct
    15481 ggcaacgaag atcagctacg cagtgcgatt tcgaacctgg tctataacgc cgtgaatcat
    15541 acgccggaag gcacgcatat caccgtacgc tggcagcgag tgccgcacgg tgccgaattt
    15601 agcgttgaag ataacggacc gggcattgca ccggagcata ttccgcgcct gaccgagcgt
    15661 ttttatcgcg ttgataaagc gcgttcccgg caaaccggcg gtagcggatt agggttagcg
    15721 atcgtgaaac atgcggtgaa tcatcacgaa agtcgcctga atattgagag tacagtagga
    15781 aaaggaacac gtttcagttt tgttatcccg gaacgtttaa ttgccaaaaa cagcgattaa
    15841 tccgcctttg tcatctttta ttgccataag ccagccgatg ctggcttatt ttctttgcag
    15901 tcaaaatacg ggcgttagat tttacaacgg ttggtgattt tttgttcgca tgattagcca
    15961 tgtctttttc acggaaatag tgttttatac tggttggtga tttcttatcg ctatatacct
    16021 ctggttttta gatccctcct tgctttaaaa cgttataagc gtttaaattg cgcttcaggt
    16081 gctgtcatac tgactgcatt aacgcggtaa atcgaaaaac tattcttcgc cgcgcctggt
    16141 tgggagtatt tcccgctaaa attgtttaaa tataccgctg tatcatcccc agggattggc
    16201 acaaaaattt aacgttacaa caccacatcc acaggcagta tgatttatga cccatcaatt
    16261 aagatcgcgc gatatcatcg ctctgggctt tatgacattt gcgttgttcg tcggcgcagg
    16321 taacattatt ttccctccaa tggtcggctt acaggcaggc gaacacgtct ggactgcggc
    16381 attcggcttc ctcattactg ccgttggcct gccggtatta acggtagtgg cgctggcaaa
    16441 agttggcggc ggtgttgaca gcctcagcac gccaatcggt aaagtcgctg gcgtactgct
    16501 ggcaacggtt tgttacctgg cggtggggcc gcttttcgct acgccgcgta cagctaccgt
    16561 ttcctttgaa gtggggattg cgccgctgac gggtgattcc gcgctgccgc tgtttatcta
    16621 cagcctggtc tatttcgcta tcgttattct ggtttcgctc tatccgggca agctgctgga
    16681 taccgtgggc aacttccttg cgccgctgaa aattatcgcg ctggtcatcc tgtctgttgt
    16741 cgcgattgtc tggccggcgg gttctatcag cacggcgact gaggcttatc aaaacgctgc
    16801 attttctaac ggcttcgtta acggctatct gaccatggat acgctgggcg caatggtgtt
    16861 tggtatcgtt attgttaacg cggcgcgttc tcgtggcgtt accgaagcgc gtctgctgac
    16921 ccgttatacc gtctgggctg gcctgatggc gggtgttggt ctgactttgc tgtacctggc
    16981 gctgttctgt ctgggttcag acagcgcgtc gctggtcgat cagtctgcaa acggcgctgc
    17041 tattctgcat gcttacgttc agcacacctt tggcggcggc ggtagcttcc tgctggcggc
    17101 gttaatcttc atcgcctgtc tggtcacggc ggttggcctg acctgtgctt gtgcagaatt
    17161 ctttgcccag tacgtaccgc tctcttatcg tacgctggtg tttatcctcg gcggcttctc
    17221 gatggtggtg tctaacctcg gcttaagcca gctgatccag atctccgtac cggtgctgac
    17281 cgctatttat ccgccgtgta tcgcactggt tgtattaagt tttacacgct catggtggca
    17341 taattcgtcc cgcgtgattg ctccgccgat gtttatcagc ctgctttttg gtattctcga
    17401 cgggatcaaa gcatctgcat tcagcgatat cttaccgtcc tgggcgcagc gtttaccgct
    17461 ggccgaacaa ggtctggcgt ggttaatgcc aacagtggtg atggtggttc tggccattat
    17521 ctgggatcgc gcggcaggtc gtcaggtgac ctccagcgct cactaaatca ctgaacattt
    17581 gttttaacca cggggctgcg atgccccgtg gttttttatt gtgttgatgg gttaggaatt
    17641 gatggaaagt aagaataagc taaagcgtgg gctaagtacc cgccacatac gctttatggc
    17701 actgggttca gcaattggca ccgggctgtt ttacggttcg gcagacgcca tcaaaatggc
    17761 cggtccgagc gtgttgttgg cctatattat cggtggtatc gcggcgtata tcattatgcg
    17821 tgcgctgggg gaaatgtcgg tacataaccc ggccgccagc tctttctcgc gttatgcgca
    17881 ggaaaacctt ggcccgctgg caggttacat taccggatgg acctactgtt ttgaaatcct
    17941 tattgtcgcc atcgccgatg tgaccgcttt tggtatctat atgggtgtct tgttcccgac
    18001 ggtgccgcac tggatttggg tactgagcgt ggtgctgatc atttgcgccg taaacctgat
    18061 gagcgtgaag gtcttcggtg agctggaatt ctggttctcg ttctttaaag tcgccaccat
    18121 catcatcatg attgtcgccg gtttcggcat catcatctgg gggattggca acggcgggca
    18181 accgaccggt attcataacc tgtggagcaa cggcggcttc ttcagtaacg gctggcttgg
    18241 catggtaatg tcgttgcaaa tggtgatgtt tgcttacggt gggatcgaaa ttatcgggat
    18301 taccgccggt gaagcgaaag atcctgagaa atcgattccg cgtgcgatta actccgtgcc
    18361 gatgcgtatt ctggtgttct acgtcggtac gctgttcgtc attatgtcta tctacccgtg
    18421 gaatcaggtt ggcactgccg gtagcccgtt cgtactgacg ttccagcata tgggcattac
    18481 ctttgccgcc agcattctta actttgttgt gctgaccgct tcgctgtcgg caattaacag
    18541 tgacgtattt ggcgtaggcc gtatgctcca cggtatggca gagcagggca gcgcaccgaa
    18601 aattttcagc aaaacgtcgc gtcgcggtat tccgtgggtt acggtgctgg tgatgactac
    18661 cgcgctgctg tttgcggtgt atctgaacta catcatgccg gaaaacgtct tcctggtgat
    18721 cgcttcgctg gcaaccttcg ccacggtatg ggtgtggatt atgatcctgc tgtcgcaaat
    18781 cgccttccgt cgccgtttgc cgccagaaga agttaaggcg ctgaaattta aagtgccggg
    18841 tggggtagca acgaccatcg gcgggctgat tttcctgctc tttattatcg ggttgattgg
    18901 ttatcacccg gatacgcgta tctcgctgta tgtcggtttc gcgtggattg ttgtgctgtt
    18961 gattggctgg atgtttaaac gccgccacga tcgtcagctg gctgaaaacc aataatactt
    19021 gcgttctgta agccggttca ggaggaaatc ctgatccggc tattccgaaa gttatccgcc
    19081 cccgtcctcc tcccccaaat atccttcaga tgatgagtga tcctgcatta ggctatggca
    19141 aggtgatcag attttcatca caggggaatt atgatgttaa atgcatggca cctgccggtg
    19201 cccccatttg ttaaacaaag caaagatcaa ctgctcatta cactgtggct gacgggcgaa
    19261 gacccaccgc agcgcattat gctgcgtaca gaacacgata acgaagaaat gtcagtacca
    19321 atgcataagc agcgcagtca gccgcagcca ggcgtcaccg catggcgtgc ggcgattgat
    19381 ctctccagcg gacaaccccg gcggcgttac agtttcaaac tgctgtggca cgatcgccag
    19441 cgttggttta caccgcaggg cttcagccga atgccgccgg cacgactgga gcagtttgcc
    19501 gtcgatgtac ctgatatcgg cccacaatgg gctgcggatc agatttttta tcagatcttc
    19561 cctgatcgtt ttgcgcgtag tcttcctcgt gaagctgaac aggatcatgt ctattaccat
    19621 catgcagccg gacaagagat catcttgcgt gactgggatg aaccggtcac ggcgcaggcg
    19681 ggcggatcaa cgttctatgg cggcgatctg gacgggataa gcgaaaaact gccgtatctg
    19741 aaaaagcttg gcgtgacagc gctgtatctc aatccggtgt ttaaagctcc cagcgtacat
    19801 aaatacgata ccgaggatta tcgccatgtc gatccgcagt ttggcggtga tggggcgttg
    19861 ctgcgtttgc gacacaatac gcagcagctg ggaatgcggc tggtgctgga cggcgtgttt
    19921 aaccacagtg gcgattccca tgcctggttt gacaggcaca atcttggcac ggggggagct
    19981 tgtcacaact ctgaatcgcc ctggcgcgac tggtactcgt ttagtgatga tggcacggcg
    20041 ctcgactggc ttggctatgc cagcttgacg aagctggatt atcagtcgga aagtctggtg
    20101 aatgaaattt atcgcgggga agacagtatt gtccgccact ggctgaaagc gccgtggaat
    20161 atggacggct ggcggctgga tgtggtgcat atgctggggg aggcgggtgg ggcgcgcaat
    20221 aatatgcagc acgttgccgg gatcaccgaa gcggcgaaag aaacccagcc ggaagcgtat
    20281 attgtcggcg aacattttgg cgatgcacgg caatggttac aggccgatgt ggaagatgct
    20341 gccatgaact atcgtggctt cacattcccg ttgtggggat ttcttgccaa taccgacatc
    20401 tcttatgatc cgcagcaaat tgacgcccaa acctgtatgg cctggatgga taattaccgc
    20461 gcagggcttt ctcatcaaca acaattacgt atgtttaatc agctcgatag ccacgatact
    20521 gcgcgattta aaacgctgct cggtcgggat attgcgcgcc tgccgctggc ggtggtctgg
    20581 ctgttcacct ggcctggtgt accgtgcatt tattacggtg atgaagtggg actggatggt
    20641 aaaaacgatc cgttttgccg taaaccgttc ccctggcagg tggaaaagca ggatacggcg
    20701 ttattcgcgc tgtaccggcg aatgattgtg ctgcgtaaga aaagtcaggc gctgcgtcgt
    20761 ggcggctgtc aggtgctgta tgcggaagat aacgtagtgg tatttgtccg cgtgctgaat
    20821 cagcaacgtg tactggtggc aatcaaccgt ggcgaggcca gtgaagtggt gctacccgcg
    20881 tcaccgtttc tcaatgccgt gcaatggcaa tgcaaagaag ggcatgggca actgactgac
    20941 gggattctgg ctttgcctgc catttcggct acggtatgga tgaactaacg ttttataacg
    21001 ccttgtgtga cgcctgcgcc atcatccgcg gataaaactg ccagaagcgg gtttcgaggg
    21061 cgtcataatg agcgtctaaa tcgtaccagg agtcacgcag ggcatccaga cgtgggcggc
    21121 ggcttgccat gccgtttaac acgttctgga tgaaatccat atcgcgatag cgcaccagcc
    21181 actgctctga ccacaagtaa ttgttcagat tgataaaacg tggcggtgag tccggcaaaa
    21241 tcgtcatcac ttgctcgcgg gcataacaga caaattcctg tagcggaaag tccggcgaca
    21301 gctgcgacca gtggcgggaa agaaagtgat cccacatgac atccagcgta ataggcgcaa
    21361 cgcggcgcgt ttcactacga aaccactcct gtgcttcgcg gacttccggc agattgtcag
    21421 tcaatacgtc gatgcatatg aatgccagcc acgacgtcgg gcggaaaact ttcttcagga
    21481 tttccgcgta cgaaatcagc cagtaaattg ccagaaagcg agctttccgc gagatgggct
    21541 aaatgcaggt gagctaaaaa attcatcgat tatattctat ccaaaggggg gtaaaggttg
    21601 cagggagagc gccccggcac tagactaccc gactcttatt ttagtctgag tcagtgtcat
    21661 gcgcgttacc gatttctcct ttgaattgcc cgaatccctg attgcccact atcccatgcc
    21721 tgaacgcagt agctgtcgtt tactgtcgct ggacgggccg acgggcgcgc tgacgcacgg
    21781 tactttcacc gatttactcg ataagctcaa ccccggcgat cttctggttt ttaataatac
    21841 ccgcgtgatc ccggcgcgtc tgtttgggcg taaagccagc ggcggcaaga ttgaagtgct
    21901 ggttgagcgg atgctcgacg acaaacgcat tcttgcgcat attcgcgcct cgaaagcgcc
    21961 aaaacctggc gcagaactgt tgctgggcga tgacgaaagt atcaacgcaa caatgaccgc
    22021 gcgccacggc gcactgtttg aagtcgaatt taatgatgaa cgctcggtac tggatatcct
    22081 caacagcatc ggccatatgc cgctgccgcc gtatatcgat cgtccggacg aagacgctga
    22141 ccgcgaactt tatcaaaccg tttatagcga aaaaccgggc gcggttgcag ccccgaccgc
    22201 tggtctgcat tttgacgagc ctttgctgga aaaattacgc gccaaaggcg tggagatggc
    22261 gtttgtgacg ttgcacgttg gtgcgggcac cttccagccg gtgcgcgtcg acaccattga
    22321 agatcacatc atgcactcgg aatacgctga agtaccgcag gatgtggtag acgcggtact
    22381 ggcggcgaaa gcgcgcggta accgggtgat tgcggttggc accacttcag tacgttcgct
    22441 ggaaagcgcg gcacaggcgg caaaaaacga tcttatcgaa ccgttctttg acgataccca
    22501 aatctttatc tatccgggct tccagtacaa agtggtcgat gcgctggtga cgaacttcca
    22561 cttgccagag tcgacgctga ttatgctggt ttcggccttt gccggttatc aacacaccat
    22621 gaatgcctat aaagcggcgg tagaagagaa atatcgcttt tttagttacg gtgatgcgat
    22681 gtttatcacg tacaatccgc aggcaattaa tgagcgcgtc ggggagtaat tccgcggcgc
    22741 tggtttaaac gttggactgt ttttctgacg tagtggagaa aaaatgaaat ttgaactgga
    22801 caccaccgac ggtcgcgcac gccgtggccg cctggtcttt gatcgtggcg tagtggaaac
    22861 gccttgtttt atgcctgttg gcacctacgg caccgtaaaa gggatgacgc cggaagaagt
    22921 tgaagccact ggcgcgcaaa ttatcctcgg caacaccttc cacctgtggc tgcgcccggg
    22981 ccaggaaatc atgaaactgc acggcgatct gcacgatttt atgcagtgga aaggaccgat
    23041 tcttaccgac tccggcggct tccaggtctt cagccttggt gatattcgta aaatcaccga
    23101 acagggcgtt cacttccgta acccgatcaa cggcgacccg attttcctcg acccggaaaa
    23161 gtcgatggag attcagtacg atcttggttc ggatatcgtc atgatctttg atgagtgtac
    23221 gccgtatcct gctgactggg attacgcaaa acgctctatg gagatgtctc tgcgttgggc
    23281 gaagcgtagc cgtgagcgtt ttgacagtct tggtaacaaa aatgcgttat ttggtataat
    23341 tcagggcagt atttacgaag atttacgtga tatatctgtt aaaggtctgg tagatatcgg
    23401 ctttgatggc tacgctgtcg gcggtctggc tgtgggtgag ccgaaagcag atatgcaccg
    23461 tattctggag catgtgtgtc cgcaaattcc ggcagacaaa ccgcgttacc tgatgggcgt
    23521 tggtaaaccg gaagacctgg ttgaaggcgt acgtcgcggt atcgatatgt ttgactgcgt
    23581 aatgccaacc cgcaacgccc gaaatggtca tttgttcgtg accgatggcg tggtgaaaat
    23641 ccgcaatgcg aaatataaga gcgatactgg cccactcgat cctgagtgtg attgctacac
    23701 ctgtcgcaat tattcacgcg cttacttgca tcatctcgac cgttgcaacg aaatattagg
    23761 cgcgcgactc aacaccattc ataaccttcg ttactaccag cgtttgatgg cgggtttacg
    23821 caaggctatt gaagagggta aattagagag cttcgtaact gatttttacc agcgtcaggg
    23881 gcgagaagta ccacctttga acgttgatta atattaataa tgagggaaat ttaatgagct
    23941 tttttatttc tgatgcggta gcggcaacgg gtgcaccggc gcaaggtagc ccgatgtctt
    24001 tgattttgat gctggtggta ttcggtctga ttttctattt catgatcctg cgtccacagc
    24061 agaagcgcac caaagaacac aaaaagctga tggactccat tgccaaaggt gatgaagttc
    24121 tgacgaacgg tggcctggtt ggtcgcgtaa ccaaagtagc ggaaaacggc tacattgcta
    24181 tcgcgctgaa tgacaccact gaagtagtta ttaaacgtga cttcgtagct gccgtcctgc
    24241 cgaaaggcac catgaaggcg ctgtaattaa aatttttccc taagggaatt gccgtgttaa
    24301 accgttatcc tttgtggaag tacgtcatgc tgatcgtggt gattgtcatc ggtctgctgt
    24361 atgcgcttcc caacctgttt ggtgaggatc cggctgttca gatcactggt gcgcgcggag
    24421 tcgccgccag tgagcaaacg ctgatccagg tccagaaaac gttacaagaa gaaaaaataa
    24481 ctgctaagtc tgtggcactg gaagagggcg ctattcttgc gcgcttcgac tccactgaca
    24541 cccagttgcg cgctcgtgaa gcattaatgg gcgtta
//
LOCUS       DAJTMX010000055        24181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTMX010000055 DAJTMX010000000
VERSION     DAJTMX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.055"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(22..594)
                     /locus_tag="OO145_002166"
     CDS             complement(22..594)
                     /locus_tag="OO145_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5467275.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO145_002167"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO145_002167"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5467276.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO145_002168"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO145_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5467277.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO145_002169"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO145_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5467278.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO145_002170"
     CDS             complement(3556..5337)
                     /locus_tag="OO145_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5467279.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO145_002171"
     CDS             complement(5330..7102)
                     /locus_tag="OO145_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5467280.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO145_002172"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO145_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5467281.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO145_002173"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO145_002173"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5467282.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO145_002174"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO145_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO145_002175"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO145_002175"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5467283.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO145_002176"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO145_002176"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5467284.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO145_002177"
     CDS             complement(11658..12029)
                     /locus_tag="OO145_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5467285.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO145_002178"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO145_002178"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5467286.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO145_002179"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO145_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5467287.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO145_002180"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO145_002180"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5467288.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO145_002181"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO145_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5467289.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO145_002182"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO145_002182"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5467290.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO145_002183"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO145_002183"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5467291.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO145_002184"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO145_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5467292.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO145_002185"
     CDS             21800..22378
                     /locus_tag="OO145_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5467293.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO145_002186"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO145_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5467294.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6664 c         5827 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 a
//
LOCUS       DAJTMX010000056        24089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTMX010000056 DAJTMX010000000
VERSION     DAJTMX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.056"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="OO145_002187"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="OO145_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5467295.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO145_002188"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO145_002188"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5467296.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="OO145_002189"
     CDS             1531..2478
                     /locus_tag="OO145_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5467297.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="OO145_002190"
     CDS             2548..3354
                     /locus_tag="OO145_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5467298.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="OO145_002191"
     CDS             3364..3771
                     /locus_tag="OO145_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5467299.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="OO145_002192"
     CDS             3771..4265
                     /locus_tag="OO145_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5467300.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="OO145_002193"
     CDS             4331..5128
                     /locus_tag="OO145_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5467301.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="OO145_002194"
     CDS             5139..5927
                     /locus_tag="OO145_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5467302.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="OO145_002195"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="OO145_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5467303.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="OO145_002196"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="OO145_002196"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5467304.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="OO145_002197"
     CDS             complement(8370..10001)
                     /locus_tag="OO145_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5467305.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO145_002198"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO145_002198"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5467306.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="OO145_002199"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="OO145_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5467307.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="OO145_002200"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="OO145_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO145_002201"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO145_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO145_002202"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO145_002202"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5467308.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO145_002203"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO145_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO145_002204"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO145_002204"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5467309.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="OO145_002205"
     CDS             23609..24052
                     /locus_tag="OO145_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5467310.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5943 a         6147 c         6108 g         5891 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTMX010000057        23757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTMX010000057 DAJTMX010000000
VERSION     DAJTMX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.057"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..246
                     /locus_tag="OO145_002206"
     CDS             <1..246
                     /locus_tag="OO145_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5467311.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO145_002207"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO145_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5467312.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO145_002208"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO145_002208"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5467313.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="OO145_002209"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="OO145_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5467314.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="OO145_002210"
     CDS             complement(3257..3541)
                     /locus_tag="OO145_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467315.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO145_002211"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO145_002211"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5467316.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OO145_002212"
     CDS             complement(4442..5650)
                     /locus_tag="OO145_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467317.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OO145_002213"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OO145_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5467318.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OO145_002214"
     CDS             complement(6445..8130)
                     /locus_tag="OO145_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5467319.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OO145_002215"
     CDS             8400..8777
                     /locus_tag="OO145_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5467320.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO145_002216"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO145_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5467321.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OO145_002217"
     CDS             9224..9511
                     /locus_tag="OO145_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5467322.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="OO145_002218"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="OO145_002218"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5467323.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="OO145_002219"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="OO145_002219"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5467324.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="OO145_002220"
     CDS             11268..11744
                     /locus_tag="OO145_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5467325.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="OO145_002221"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="OO145_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5467326.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="OO145_002222"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="OO145_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5467327.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="OO145_002223"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="OO145_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5467328.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="OO145_002224"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="OO145_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5467329.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="OO145_002225"
     CDS             16301..16789
                     /locus_tag="OO145_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5467330.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="OO145_002226"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="OO145_002226"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5467331.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO145_002227"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO145_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5467332.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO145_002228"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO145_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5467333.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO145_002229"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO145_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5467334.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO145_002230"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO145_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5467335.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO145_002231"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO145_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5467336.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="OO145_002232"
     CDS             complement(22241..22756)
                     /locus_tag="OO145_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5467337.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..>23757
                     /locus_tag="OO145_002233"
     CDS             23405..>23757
                     /locus_tag="OO145_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5467338.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIR"
BASE COUNT         5886 a         5811 c         6100 g         5960 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggct gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacg
//
LOCUS       DAJTMX010000058        22942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTMX010000058 DAJTMX010000000
VERSION     DAJTMX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.058"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            389..2047
                     /locus_tag="OO145_002234"
     CDS             389..2047
                     /locus_tag="OO145_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5467339.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OO145_002235"
     CDS             complement(2044..3000)
                     /locus_tag="OO145_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5467340.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OO145_002236"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OO145_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5467341.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OO145_002237"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OO145_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5467342.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO145_002238"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO145_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5467343.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OO145_002239"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OO145_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5467344.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OO145_002240"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OO145_002240"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5467345.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OO145_002241"
     tRNA            complement(9859..9946)
                     /locus_tag="OO145_002241"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO145_002242"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO145_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5467346.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO145_002243"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO145_002243"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5467347.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO145_002244"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO145_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5467348.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO145_002245"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO145_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5467349.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO145_002246"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO145_002246"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5467350.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OO145_002247"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OO145_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5467351.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OO145_002248"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OO145_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5467352.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OO145_002249"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OO145_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5467353.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..>22942
                     /gene="rarA"
                     /locus_tag="OO145_002250"
                     /gene_synonym="mgsA"
     CDS             22003..>22942
                     /gene="rarA"
                     /locus_tag="OO145_002250"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5467354.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQV"
BASE COUNT         5728 a         5666 c         6174 g         5374 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt gg
//
LOCUS       DAJTMX010000059        22746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTMX010000059 DAJTMX010000000
VERSION     DAJTMX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.059"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..603)
                     /locus_tag="OO145_002251"
     CDS             complement(<1..603)
                     /locus_tag="OO145_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator family protein"
                     /protein_id="HCR5467355.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPR"
     gene            923..1483
                     /gene="nudE"
                     /locus_tag="OO145_002252"
     CDS             923..1483
                     /gene="nudE"
                     /locus_tag="OO145_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5467356.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(1648..4200)
                     /gene="mrcA"
                     /locus_tag="OO145_002253"
     CDS             complement(1648..4200)
                     /gene="mrcA"
                     /locus_tag="OO145_002253"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5467357.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            4320..5099
                     /gene="hofM"
                     /locus_tag="OO145_002254"
     CDS             4320..5099
                     /gene="hofM"
                     /locus_tag="OO145_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5467358.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            5099..5638
                     /gene="hofN"
                     /locus_tag="OO145_002255"
     CDS             5099..5638
                     /gene="hofN"
                     /locus_tag="OO145_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5467359.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            5622..6062
                     /gene="hofO"
                     /locus_tag="OO145_002256"
     CDS             5622..6062
                     /gene="hofO"
                     /locus_tag="OO145_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5467360.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            6052..6458
                     /gene="hofP"
                     /locus_tag="OO145_002257"
                     /pseudo
     CDS             6052..6458
                     /gene="hofP"
                     /locus_tag="OO145_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            6370..7608
                     /gene="hofQ"
                     /locus_tag="OO145_002258"
     CDS             6370..7608
                     /gene="hofQ"
                     /locus_tag="OO145_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5467361.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            8009..8530
                     /gene="aroK"
                     /locus_tag="OO145_002259"
     CDS             8009..8530
                     /gene="aroK"
                     /locus_tag="OO145_002259"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5467362.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            8587..9675
                     /gene="aroB"
                     /locus_tag="OO145_002260"
     CDS             8587..9675
                     /gene="aroB"
                     /locus_tag="OO145_002260"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5467363.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            9767..11059
                     /gene="damX"
                     /locus_tag="OO145_002261"
     CDS             9767..11059
                     /gene="damX"
                     /locus_tag="OO145_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5467364.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            11166..12002
                     /gene="dam"
                     /locus_tag="OO145_002262"
     CDS             11166..12002
                     /gene="dam"
                     /locus_tag="OO145_002262"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5467365.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            12020..12697
                     /gene="rpe"
                     /locus_tag="OO145_002263"
     CDS             12020..12697
                     /gene="rpe"
                     /locus_tag="OO145_002263"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5467366.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            12690..13448
                     /gene="gph"
                     /locus_tag="OO145_002264"
     CDS             12690..13448
                     /gene="gph"
                     /locus_tag="OO145_002264"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5467367.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            13441..14445
                     /gene="trpS"
                     /locus_tag="OO145_002265"
     CDS             13441..14445
                     /gene="trpS"
                     /locus_tag="OO145_002265"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5467368.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            14715..15620
                     /locus_tag="OO145_002266"
     CDS             14715..15620
                     /locus_tag="OO145_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467369.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            15637..15999
                     /locus_tag="OO145_002267"
     CDS             15637..15999
                     /locus_tag="OO145_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5467370.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            16083..17246
                     /locus_tag="OO145_002268"
     CDS             16083..17246
                     /locus_tag="OO145_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5467371.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            17247..18473
                     /locus_tag="OO145_002269"
     CDS             17247..18473
                     /locus_tag="OO145_002269"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5467372.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            18470..19348
                     /locus_tag="OO145_002270"
     CDS             18470..19348
                     /locus_tag="OO145_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5467373.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            19359..19712
                     /locus_tag="OO145_002271"
     CDS             19359..19712
                     /locus_tag="OO145_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5467374.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            19724..21028
                     /locus_tag="OO145_002272"
     CDS             19724..21028
                     /locus_tag="OO145_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5467375.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            21040..22125
                     /locus_tag="OO145_002273"
     CDS             21040..22125
                     /locus_tag="OO145_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5467376.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(22285..>22746)
                     /gene="phoB"
                     /locus_tag="OO145_002274"
     CDS             complement(22285..>22746)
                     /gene="phoB"
                     /locus_tag="OO145_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5467377.1"
                     /translation="TDFGVSQGKSTKEKVIEQERVSAAPFCEKLNIPGNSEVFHLCRV
                     MYLDKEPLFIDSSWIPLSRYPDFDEIYVEGSSTYQLFQERFDTRVVSDKKTIDIFAAT
                     RPQAKWLKCELGEPLFRISKIAFDQNDKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         5324 a         5655 c         6363 g         5404 t
ORIGIN      
        1 gcgcggacga ctcagcgcat attcttcatg cgtttctttg cgaatattga gtagctcgat
       61 ttgctcactc tcttccccgc gaatcgacgc ctgcgtggac ggaacaggtt gcaaggcata
      121 gccgcgcgtt tcctgcatgt actcctgcag cgtatgaccg ttgagtgaaa taaccaacgg
      181 cagcgaatcg gtatgaatca gttcaacggt attctcatcg ttgatgtact gttcccagaa
      241 ggggggcagg tggacttcta ccgaatcgag gtagtaacgc catttattcg gatcgtcggt
      301 agagatgccg taacgtgtga tagcgtgtgt cagcatcatg acgttgttgc tttcggcatt
      361 cagcgccaga gagatcggtg ccgcgctagc ttccgttggg ccaggcacct gtaatacctg
      421 cgtcaggctc tcaagataat tttcaacggc gctacgttct tcaggtgtga gtttacgcgt
      481 ttgcgcatcc gcgaaggcgt tggtccaggg cagctgacgc cgtctggatc gcacttttat
      541 cagccatccc gcaagtagtg agcaggccag caaagcagct aaaaaaatca caatggtgct
      601 catgctttcc ccatcttact tagtctgtca ggcgtggtca gtcgcaccgg acacaataca
      661 tgattctgcg gttggctatc ggcaagcgtg gagcttaact tgagcatcat taagcacatc
      721 acagtaaccg ctgatcatag caaaccctct gaaggcggaa tatcagcaaa ttcctggagt
      781 tatttcaacc tattgacttt taatttgatt tgaggacgat tatttattaa gttacataaa
      841 tgtaaataac atgcaattca cattgccgaa agcgtgtccg atatcgcaca ataaccgctg
      901 atttcacagc atagatccca cgatgaacaa atcattacaa aaacccacca ttctgaatgt
      961 tgaaactgta gcccgttccc gactgtttac cgtcgagagc gtggatctgg agttcagcaa
     1021 tggcgtgcgg cgtgtttatg aacgaatgcg tccaaccaac cgggaagcag tgatgattgt
     1081 gccgattgtg gacgatcacc tgatcctgat ccgcgaatac gcagtgggaa ctgaatccta
     1141 cgaattaggt ttttcgaaag gattaattga tccgggtgaa agcgtctacg aagccgctaa
     1201 ccgcgagcta aaagaagagg ttggatttgg tgcgaacgat ctgacttttt tgaagaagct
     1261 cagcatggca ccgtcttact tttccagcaa aatgaatatc gtggtcgcgc aagatctcta
     1321 cccggaatcg ctggaaggcg atgagccaga gccgctacca caggtgcgct ggccgctggc
     1381 gcatatgatg gatttgctgg aagaccctga cttcaacgaa gcgcgtaatg tcagcgcgct
     1441 gttcctcgtg cgcgaatggt tgaaagggca ggggcgagtg taaatgcgtg aagtttgcct
     1501 gatgcacaac gcttatcagg cttatggggt tcctgcaata tattgatttt tcacgatttt
     1561 gttagccgga taacgcgttc acccgcatcc ggcataaaca aatgcacttt gtcagccaac
     1621 tgaaaaggcg ccgaagcgcc tttttaatca gaacaattcc tgtgcctcac cattatcgat
     1681 aatggtcgtt cccacctcgt gcaccgcctg ttgtgtcggc tgcgtacctt cgatgaaata
     1741 ctcttcgcgg ctgttgccac cattagctaa ctgcccggtg ctgcgatcga tattcaccgt
     1801 cacaataccc ggaggcggcg tcagcggctg ctccggcaca ccttcaagaa cggctttcat
     1861 ataagcgtcc catgcaggct gggcactctt ggcaccgcct tcgtaacctg agatctgatc
     1921 tttaatcgct ccggaagccg ttgtatgacc gagattacga cggtgatcat caaagccaat
     1981 ccagaccgag gtcacaacgc ccggaccgta acccgagaac cacgcatctt tcgaactgtt
     2041 agtggtaccg gttttcccgc cgatatcgcg acgctgcaaa tcacgacctg cacgccagcc
     2101 ggtaccctgc cagcctggtt caccaaagat attggtgttc aaagcactct taatcaggaa
     2161 tgccagcgga gtgttgatga cgtgcggtgc gtactcctgc gcgccagtct tcgccactaa
     2221 cgcctgattt gcctgctcca gctgcggcat tggtacagaa acattctgct gctcgcggga
     2281 gatagcgaca tcttcaacat cgttattttc cagcacgttc gatttctgcg tatcaccgta
     2341 aatcactgga atatcgcatt ccgggcaggc tactttcggt ttcgcttcga aaatcacgcc
     2401 gccctgatcg ttttcaattt tgctgataaa ccacgggtcc actaggaagc cgccgttcgc
     2461 catgaccgcg tagccgcgcg ccacctgcat tggggtgaag gacgctgaac ccagcgccag
     2521 cgattcggtg tggacaatgt tttgcgccgg gaagccgaag cgttgcagat attctgcagc
     2581 gtagtcgacg cccatcgccc gcattgcacg taccatcacc acgtttttcg actgacccag
     2641 cccctgacgt aagcgaattg gaccagcata ctgcggtggt gagttcttcg gctgccagtc
     2701 agaaccggca cctgcatccc agcgagaaat tggcacatcg ttcaacatac ttgccagcgt
     2761 cagaccttta tccatcgccg cggtgtagag gaacggtttg atgttggaac ccacctgacg
     2821 cagtgcctgg gtggcgcggt taaacttgct ctgattgaaa tcaaagccac cgaccagcgc
     2881 cataacggca ccattttgcg gattgatcga caccagcgcc gagttcactt ccggcacttg
     2941 tgccagccac catgcatcgc caacctgacg aacccagatt tgctgacccg tttgcagaac
     3001 atcggtcact ttacgcggcg tcggtccctg ctgagtatcc gaacggtaag gacgcgccca
     3061 gcgaacgcct tccatactca atgcgacggt cgacccgtcc gccagcatcg ccgtcgctcc
     3121 ctgaggattg gcgctggtga ctgcggcagg cagcagcgga ccataggtcg gcagcgcctt
     3181 cagcgtatcg gtaatcttgt tgttatccca cgccgactcg cccactttcc acagcacatt
     3241 tgccgggccg cgatagccgt ggcgcatgtc gtagtccagc acgttattac gtaccgcctg
     3301 ctgcgcggcc tgctgcactt tgcgggtgat ggtggtgtaa atgcgataac cgtcttcata
     3361 ggcactttcg ccataacggt tatacatctc ctggcgcacc atttcgctca ggtacggcgc
     3421 agagaaagca atctccggcg cgtgataatt agcgttaatc gcctcagtgc gtgtctgatc
     3481 gaactgttgc tgggtgatat acccttcatc cagcatccgc gacagtacga cgttacgccg
     3541 cgcgacggca cgatccattg agtagagcgg attaaaggtg gaaggcgctt tcggcaaccc
     3601 ggctatcacc gccatttcgt tcagcgtcag ttggtcgacc gttttaccga aatagacttg
     3661 tgccgcagca ccgacaccat aggcgcggta accaaggtaa atcttgttca gataaagctc
     3721 gaggatttcg tctttcgtca gcaactgttc aatgcgaatc gcgaggaaga cttccttaat
     3781 cttacgcatc atcgtgcgtt ctggactgag gaagaaattt ctcgccagct gctgggtaat
     3841 ggtactcgcc ccttgtgacg cgtggccgga gaacagcgcc acacttgccg cacggaagat
     3901 ccccaccggg tcaacgccgt gatgctcgta gaagcggctg tcttccgtcg cgataaaggc
     3961 tttcaccatc tccggtggga tttgatccaa cgtaaccgga atacgacgtt tctcaccgta
     4021 ttgagcaatc agctcgccat cggcgctgta aatctgcatc ggaatttgca ggcgaacatc
     4081 ttttaatgtc accacatccg gcagttgtgg ctcgatgtag cggtataggc cataaatcga
     4141 gcctgctccc agcagaatgc aacagactgc aaggatcaaa aaatacttta cgaacttcac
     4201 tggaaatttc ccatttagtt tcatttgggc agtttataaa caaacgcgcg gtagtataaa
     4261 ggcaagccag acgcattgat atacccgtca gagtgacggg tgataaggag atcatcacaa
     4321 tgacatttaa gatctggcaa attggtttgc atttacaaca gcaagaagcg gcagcggttg
     4381 cgatcgtgcg gggcgcaaaa gaatgctttt tgcaacgctg gtggcggttg ccgctggcgc
     4441 acgacattat caaagatggg cggattgttg atgcgcagca gctggctaaa acgttgttac
     4501 cgtggagtcg cgaactgccg cagcgtcatc acattatgtt ggcgtttccc gccagtcgca
     4561 cattacagcg gtcatttccg cgcccgtcga tgtcccttgg tgagcgggag caaacggcct
     4621 ggctgtcagg gacgatggcc cgcgagctgg atatggatcc ggactccctg cgcttcgatt
     4681 acagcgaaga ctcactcagc cccgcttata acgtgactgc cgcgcaaagc aaagagctgg
     4741 caacgctgct tacgctggca gaaaggttgc gtgttcatgt tagtgcgatc accccggatg
     4801 ccagtgcatt acagcgattc ctgccttttt taccttctca tcagcaatgt ctggcctggc
     4861 gtgataacga acagtggctg tgggcgacgc gctatagctg ggggcgcaaa ctggcggtgg
     4921 ggatgactag cgcgaaggag ctggcggcag cgttatccgt tgatcccgac agcgtcgcga
     4981 tatgtggcga aggcggattt gacccctggg aggccgtttc tgttcgtcag ccgccgctac
     5041 cgccgcctgg tggagacttt gccatcgcgc tggggctggc gcttgggaag gcgtactgat
     5101 gaatccgcca attaattttt tgccctggcg acagcaacgc cggaccgctt ttctgcgttt
     5161 ctggttgctg atgttcgttg cgcctctgct gctggccgtc gggataacgc taatactgcg
     5221 tctgacaggc agcgccgaag cgcgcataga cgcagttttg cttcaggcgg aacaacaact
     5281 cgcccgcagc ttacagataa cgaagccacg tttgctggag caacagcagt tacgcgaaca
     5341 gcgttcgcaa cggcagcgcc agcgacaatt tacccgcgac tggcaatctg cgctggaagc
     5401 actggcggcg cttttacccg agcacgcctg gctgacaacg ataagctggc agcagggaac
     5461 gctggagatc aaggggctga caacaagcat taccgcgtta aacgcactag aaacgtcact
     5521 ccgccaggat gcgtcttttc atctcaatca gcggggagcc acgcagcagg atgcgcaggg
     5581 acgctggcaa tttgagtatc agttaacaag gaaggttagc gatgaacatg ttctttgact
     5641 ggtggttcgc cacatcaccc cgcctccgcc agttttgctg ggcattctgg ttgctgatgt
     5701 tagttacgct catttttcta tcatcgacac accatgaaga gcgcgacgca ttaattcgac
     5761 tacgggcaag tcatcaccag cagtgggccg cactgtatcg cctggtagac accactccct
     5821 tcagcgagga aaaaacgctg cccttttcgc cactggattt tcagttatcc ggcgcgcaac
     5881 tggtttcctg gcatccatcc gcgcagggag gtgagttggc gttgaaaacg ctgtgggaag
     5941 cagtgccgtc ggcatttaca cggctggcag agcgcaacgt cagcgtgagc cgtttttcgt
     6001 taagcgtgga aggtgatgat cttttgttca cgctacaact ggagatgccg catgagggtt
     6061 aaacgctggt tgttggcagg tattgcattg tgccttttaa ccggtatgcg tgaccctttt
     6121 aaatcgccgg aagatctata tgccggatta gcgaacttag ccagtggcgc tatcagggga
     6181 tggtagggcg aggcgagcgc atcatcggtg taataaaaga cgggcaaaag aaatggcgac
     6241 gggtgcagca aaacgatgtg ctggaaaacg gctggacaat tttacagctg acgccagacg
     6301 tactaacgct gggtaccggg acaaactgcg aaccgccaca atggttgtgg caacggcaag
     6361 gagatacaaa tgaagcaatg gatagccgca ctactgttga tgctgatacc cggcgtacag
     6421 gcggcaaagc cgcaaaaagt gacgctgatg gtggatgacg ttccggtagc tcaggtgttg
     6481 caggcgctgg ctgaacagga gaagttgaac ctggtcgtgt cgccagacgt cagcggtacg
     6541 gtgtcgttac atctaacaga tgttccctgg aagcaggcac tacaaactgt agtgaaaagc
     6601 gccggactga taacgcggca ggaaggcaac attctctcag tgcattccat tgcctggcag
     6661 aatgacaata tcgcccgcca ggaggcggag caggcgcggg cgcaggcaaa tctgccgctg
     6721 gaaaatcgca gtataaccct gcaatacgcc gacgcgggag aactggcgaa agcgggggag
     6781 aagctactga gtgccaaagg gagtatgacc gtcgataaac gcaccaatcg ccttttgcta
     6841 cgagataaca aaacggcgtt aagcgcgctt gaacagtggg tagcgcaaat ggatctgccg
     6901 gtcgggcagg ttgagctgtc ggcgcatatt gtcaccatta atgaaaaaag tttgcgtgag
     6961 ttaggcgtga aatggacgct ggccgatgcg caacacgctg gtggcgttgg gcaagtcacc
     7021 acgcttggta gcgacctctc cgtagcgacg gcgacaacgc atgtcggttt taacattggg
     7081 cgcatcaacg gacgcttgct ggatcttgag ctttccgcgc tcgaacaaaa acagcagctg
     7141 gatattatcg ccagtccgcg tctgctggcc tcacatcttc agcctgccag cattaaacag
     7201 gagagcgaaa ttccatatca ggtttccagc ggggaaagtg gcgcgacgtc ggtggaattt
     7261 aaagaggccg tcctggggat ggaggtcacg cccacggtgt tacaaaaagg tcgcatccgg
     7321 ctgaaattac acatcagcca gaacgttccg gggcaggtgc tacagcaggc cgatggcgaa
     7381 gtgctggcga ttgataagca ggagatcgaa acgcaggtcg aggtaaaaag cggagaaacg
     7441 ttggcgctgg gcggcatttt tacccgtaaa aataaatcgg gtcaggatag cgtaccgttg
     7501 cttggcgaca ttccctggtt cgggcaatta tttcgtcatg acggaaaaga agatgaacga
     7561 cgcgagttag tggtgtttat cacgccacga ctggtttcca gtgagtaaac agccgtaaaa
     7621 gcggtaatgt ttttatgctg aacgtgtttc atctatttga cgcgcgcagg tatttagcat
     7681 acaaggagta ccgatttgag agttggtgct cttcgctgcc tgcgttccat gatgatgatt
     7741 tatcattcag gcggcatttt gctgtctttt ttacgctaat cttacccggt gatttatcgc
     7801 cagagcggtg gtagcaaggc agcgcgcttg cagcgaccag atatgcagag ggatgggtga
     7861 tttattcagt tgccaaaccc gctggagtat tgagataatt ttcagtctga ctctcgcaat
     7921 atcttatgag gtttcagttc atgtcctgcg gcgctctctg agcgaagcgg gtttatcatt
     7981 aacgaatagt cttagtagta ccgaaaaaat ggcagagaaa cgcaatatct ttctggttgg
     8041 gcctatgggt gccggaaaaa gcactattgg gcgccagtta gctcaacaac tcaatatgga
     8101 attttacgat tccgatcaag agattgagaa acgaaccgga gctgatgtgg gctgggtttt
     8161 cgatttagaa ggcgaagaag gcttccgcga tcgcgaagaa aaggtcatca atgagttgac
     8221 cgagaaacag ggtattgtgc tggctactgg cggcggctct gtgaaatccc gtgaaacgcg
     8281 taaccgtctt tccgctcgtg gcgttgtcgt ttatcttgaa acgaccatcg aaaagcaact
     8341 tgcacgcacg cagcgtgata aaaaacgccc gttgctgcac gttgaaacac cgccgcgtga
     8401 agttctggaa gcgttggcca atgaacgcaa tccgctgtat gaagagattg ccgacgtgac
     8461 cattcgtact gatgatcaaa gcgctaaagt ggttgcaaac cagattattc acatgctgga
     8521 aagcaactaa ttctggcttt atatacactc gtctgcgggt acagtaatta aggtggatgt
     8581 cgcgttatgg agaggattgt cgttactctc ggggaacgta gttacccaat taccatcgca
     8641 tctggtttgt ttaatgaacc agcttcattc ttaccgctga aatcgggcga gcaggtcatg
     8701 ttggtcacca gcgaaaccct ggctcctctg tatctcgata aggtccgcgg cgtacttgaa
     8761 caggcgggtg ttaacgtcga tagcgttatc ctccctgacg gcgagcagta taaaagccta
     8821 gctgtactcg ataccgtctt tacggcgttg ttacaaaagc cgcatggtcg cgatactacg
     8881 ctggtggcgc ttggcggcgg cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat
     8941 cagcgcggtg ttcgtttcat tcaagtcccg acgacgttac tgtcgcaggt cgattcctcc
     9001 gttggcggca aaactgcggt caaccatccc ctcggtaaaa acatgattgg cgcgttctac
     9061 cagcctgctt cagtggtggt ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta
     9121 gcgtcggggc tggcagaagt catcaaatac ggcattattc ttgacggtgc gttttttaac
     9181 tggctggaag agaatctgga tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt
     9241 attcgccgtt gttgtgaact gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg
     9301 ttacgtgctt tactgaatct gggacacacc tttggtcatg ccattgaagc tgaaatgggg
     9361 tatggcaatt ggttacatgg tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg
     9421 tcggaacgtc tcgggcagtt tagttctgcc gaaacgcagc gtattataac cctgctcacg
     9481 cgggctgggt taccggtcaa tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat
     9541 atgctgcgtg acaagaaagt ccttgcggga gagatgcgct taattcttcc gttggcaatt
     9601 ggtaagagtg aagttcgcag cggcgtttcg cacgagcttg ttcttaacgc cattgccgat
     9661 tgtcaatcag cgtaacaaca agaaaggtca ggccgcttat caagcggtct attagcttca
     9721 ggttaattgc aacgtggtaa gcattaacct tttagtgggg tgttaaatgg atgaattcaa
     9781 accagaagac gagctgaaac ccgatcccag cgatcgtcgt actggtcgtt ctcgtcaatc
     9841 ttctgaacgt tctgagcgta ctgaacgtgg cgaaccgcag atcaattttg atgatattga
     9901 acttgatgac actgacgatc gccgtccgac tcgtgcgcaa aaagagcgca atgaggaacc
     9961 ggaaatcgaa gaagaaattg acgaatccga agatgaaacc gtggatgaag agcgcgtaga
    10021 gcgtcgtccg cgtaagcgca aaaaagcagc cagtaaaccc gcttctcgtc agtatatgat
    10081 gatgggcgtc ggcattctgg ttctactgct gttgatcatc ggtatcggtt ctgcgctaaa
    10141 agccccctcg accacttcca gcgatcaaac cgcgtctggc gagaagagta ttgatcttgc
    10201 tggcaatgcg accgatcagg cgaatggcgt gcagccagcg ccgggaacca cgtctgcgga
    10261 aaatactcag caggatgttt ctctgccacc gatctcttct acgccgactc aagggcaaac
    10321 cccggtggca acggatggtc aacaacgtgt tgaagtgcag ggtgacctga acaatgcgct
    10381 gacccagcca caaaatcagc aacagctgaa caatgtggcg gtcaattcca cgttgccgac
    10441 tgaacccgca acggttgcgc ctgttcgcaa tggcaatgca tcgcgtgaca cggcgaaaac
    10501 gcaaaccgct gaacgtccgt ccactacgcg cccagttcgt cagcaggcgg tgattgaacc
    10561 gaaaaaaccg caagcaaccg tgaaaacgga gccgaagccg gtagcacaga cgccgaagcg
    10621 tactgaacct gaaccagctg cccctgtggc gagcacgaag gcaccggctg cgacttctac
    10681 gccagcacca aaagagacgg cgactacggc tccagtacag acggcatccc cggcgcaaac
    10741 cacggcaaca ccagccgctg gagggaagac cgcaggtaat gttggttcgt tgaaatcggc
    10801 accgtccagc cattacactc tgcagctgag cagttcctct aactacgaca acctgaacgg
    10861 ttgggcgaag aaagagaatc tgaaaaacta cgttgtctat gaaacgacgc gtaatggtca
    10921 gccgtggtat gtcctggttt ctggcgtgta cgcttcgaaa gaagaggcga aaaaagcggt
    10981 atctacattg ccagcagatg ttcaggccaa aaacccgtgg gcgaaaccgc tgcgtcaggt
    11041 acaggccgat ctgaagtaat caaggttatc tcccgcaatg gtttatcgtt gcgggagctg
    11101 cctgaagcgc tggatgctgt cggagctttc tccacagccg gagaaggtgt aattagttaa
    11161 tcggcatgaa gaaaaatcgc gcttttttga agtgggcagg gggcaagtat cccctgcttg
    11221 atgatattaa acggcatttg cccaagggcg aatgtctggt tgagcctttt gtaggtgccg
    11281 ggtcggtgtt tctcaacacc gacttttctc gttatatcct tgccgatatc aatagcgacc
    11341 tgatcagtct ctataacatt gtgaagatgc gtactgatga gtacgtacag gccgcacgcg
    11401 agctgtttgt tcccgaaaca aattgcgccg aggtttacta tcagttccgc gaagagttca
    11461 acaaaagcca ggatccgttc cgtcgggcgg tactgttttt atatttgaac cgctacggtt
    11521 acaacggcct gtgtcgttac aatctgcgcg gtgagtttaa cgtgccgttt ggccgctaca
    11581 aaaaacccta tttcccggaa gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct
    11641 ttttctattg tgagtcttac gccgatagca tggagcgcgc agatgatgca tccgtcgtct
    11701 attgcgatcc gccttatgca ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa
    11761 acagttttac gcttgaacaa caagcgcatc tggcggagat cgccgaaggt ctggttgagc
    11821 gccatattcc agtgctgatc tccaatcacg atacgatgtt aacgcgtgag tggtatcagc
    11881 gcgcaaaatt gcatgtcgtc aaagttcgac gcagtataag cagcaacggc ggcacacgta
    11941 aaaaggtgga cgaactgctg gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaat
    12001 aattctcaag gagaagcgga tgaaacagta tttgattgcc ccctcaattc tgtcggctga
    12061 ttttgcccgc ctgggtgaag ataccgcaaa agccctggca gctggcgctg atgtcgtgca
    12121 ttttgacgtc atggataacc actatgttcc caatctgacg attgggccaa tggtgctgaa
    12181 atccttgcgt aactacggca ttaccgcccc tatcgacgta catctgatgg tgaaacccgt
    12241 cgatcgcatt gtgcctgatt tcgctgccgc tggtgccagc atcattacct ttcatccaga
    12301 agcctccgag catgttgacc gcacgctgca actgattaaa gaaaatggct gtaaagcggg
    12361 tctggtattt aacccggcga cacctctgag ctatctggat tacgtgatgg ataagctgga
    12421 tgtgatcctg ctgatgtccg tcaaccctgg tttcggcggt cagtctttca ttcctcaaac
    12481 actggataaa ctgcgcgaag tacgtcgccg tatcgacgag tctggctttg acattcgtct
    12541 ggaagtggac ggtggcgtga aggtgaacaa cattggcgaa atcgctgcgg cgggcgcgga
    12601 tatgttcgtc gccggttcgg caatcttcga ccagccagac tataaaaaag tcattgatga
    12661 aatgcgcagt gaactggcaa aggtaagtca tgaataagtt tgaagatatt cgcggcgtcg
    12721 cttttgatct tgatggtacg ctggtcgaca gtgctcctgg tcttgctgct gcggtagata
    12781 tggcgctgta tgcgctggag ttgcccgtcg caggtgaaga acgcgttatt acctggattg
    12841 gtaacggcgc agatgttctg atggagcgcg cattgacctg ggcgcgtcag gaacgtgcga
    12901 ctctgcgtaa aacaatgggg aaaccgcccg ttgatgacga cattccggca gaagaacagg
    12961 tacgtattct gcgtaaactg ttcgatcgct actatggcga ggttgccgaa gaggggacgt
    13021 ttttgttccc gcacgttgcc gatacgttgg gcgcgttgca ggctaaaggc ctgccgctag
    13081 gcttggtcac caacaaaccg acgccgttcg tcgcgccgct gctcgaagcc ttagatattg
    13141 ccaaatactt tagcgtggtt atcggcggcg atgatgtgca aaacaaaaaa ccgcatccgg
    13201 acccgctgtt actggtggct gagcggatgg gaattgcccc acaacagatg ctgtttgtcg
    13261 gcgactcacg gaatgatatt caggcggcaa aagcggcagg ttgcccatca gttggcttaa
    13321 cctacggata caactacggc gaggctatcg atctcagcca gcctgatgta atttatcagt
    13381 ctataaatga ccttctgccc gcattagggc ttccgcatag cgaaaatcag gaatcgaaaa
    13441 atgactaagc ccatcgtttt tagtggcgca cagccctcag gtgaattgac cattggtaac
    13501 tacatgggtg cgctgcgtca gtgggtaaac atgcaggatg actaccattg catttactgt
    13561 atcgttgacc aacacgcgat caccgtgcgc caggatgcac agaagctgcg taaagcgacg
    13621 ttggatacgc tggccttgta tctggcttgt ggtatcgatc ctgagaaaag caccattttt
    13681 gttcagtccc acgtaccaga acatgcgcag ttaggctggg cactgaactg ctatacctac
    13741 ttcggcgaac tgagtcgcat gacgcagttt aaagataaat ctgcgcgtta tgccgagaac
    13801 atcaacgctg gtctgtttgg ctatccggtg ctgatggctg cggacatcct gctgtatcaa
    13861 actaatctgg taccggtggg tgaagaccag aaacagcacc tggaactgag tcgcgatatc
    13921 gcccagcgtt tcaacgcgct gtatggcgag atctttaagg tgccggagcc gtttattccg
    13981 aaatctggcg cgcgcgtaat gtcgctgctg gagccgacca agaagatgtc caagtctgac
    14041 gataaccgca ataacgttat cggcctgctg gaagatccga aatcggtagt gaagaaaatc
    14101 aaacgcgcgg tcactgactc cgacgagccg ccggtagttc gctacgatgt gcagaacaaa
    14161 gcgggcgttt ccaacctgct ggatatcctt tctgcggtaa cgggccagag catcccagaa
    14221 ctggaaaaac agttcgaagg caagatgtat ggtcatctga aaggtgaagt ggctgatgcc
    14281 gtttccggta tgctgactga attgcaggaa cgctatcacc gtttccgcaa cgatgaagcc
    14341 ttcctgcaac aggtgatgaa agacggcgca gaaaaagcca gtgtacacgc ttcccgtacg
    14401 ctgaaagcgg tgtacgaagc gattggtttt gtggcgaagc cgtaatatca aaacgctcgg
    14461 acggtcccct cgcccctttg gggagagggt tagggtgagg ggagcaggca agcgccagtg
    14521 ggaattaccc tttgctcgca ttgtcgaata aatcacttcg tcatcactct gaaaccgggg
    14581 aaacccggtt tttttatcct cttttcgcaa caacccgaaa aaatgtgaag cgcctcgccg
    14641 tttccacatc tcgtcgtcgc cacttatctt cattttaatg aagatataaa tattcatttt
    14701 attgaaaata tattatgcgt cgaacgttta tcaaaaaaga aggcgtcgtc atcacgacac
    14761 tggcccgtta tttgttgggt gaaaaatgcg gtaatcgact gaaaaccata gatgagctgg
    14821 caactgaatg ccgttcatcg gttggcctga cgcaggccgc gttgaaaacg ctggaatcaa
    14881 gcggagcgat acggattgaa cgccgtgggc gcaatggcag ttatcttgtc gagatggata
    14941 acaaagcatt gctgagtcat gtggatatca acaacgtggt atgtgcgatg cctttgccct
    15001 atacccgctt gtacgaaggt ttagcgagcg gattgaaagc ccagtttgat ggcattcctt
    15061 tttactatgc gcatatgcgc ggcgcggata ttcgcgtgga gtgtttactt aacggtgtct
    15121 atgacatggc agtagtttcc cgcctggcgg cggaaagtta tctcagccaa aacaatttgt
    15181 gcatcgccct cgagcttggc ccgcatacgt atgttggtga acatcaactg atatgccgca
    15241 aaggtgagtc tggaaatgtt aagcgagtag gtctcgatag tcgttcggcg gatcagaaaa
    15301 tcatgaccga tgtctttttt ggcgatagtg atgtggaacg agtcgatctc tcttatcacg
    15361 agagtttaca acgcattgtt aaaggcgatg tcgatgcggt gatctggaac gtggtggcgg
    15421 aaaacgaact gaccatgctg ggattagagg cgacgccgct aacagacgat ccgcgatttt
    15481 tacaggccac cgaagccgtg gtcctgacgc gagtcgatga ttacccaatg caacaattgc
    15541 tgcgtgctgt tgtagataaa cacgccctgc ttgcccatca acaacgggtg gtgagtgggg
    15601 aacaggaacc cagttattaa acgaaaggca tctgatatgg aaaccagact caacctgctt
    15661 tgcgaggcag gcgttattga taaggacgtc tgcaaaggca tgatgcaggt cgtcaacgta
    15721 ctggaaaaag agtgccatct gccggtgcgc agtgagcaag gaacgatggc gatgacacat
    15781 atggcgagtg cactgatgcg cagtcgccgt ggtgaagaaa tagagccgct ggataacgag
    15841 ttgctggcgg aactggcgca atccagccac tggcaagccg ttgtgcaatt gcatcaggag
    15901 ttgttgaagg aattcgctct ggaagttaac ccgtgtgaag aaggctattt tctggccaat
    15961 ctttatggat tatggatggc tgctaacgaa gaggtttgaa gctcccgaca aatccacgtc
    16021 gcacaaccta aatattcaaa aaaatgaata tttaattcaa ggaacagaaa cgaggcaaca
    16081 agatgtttgt agaagcattg aaacgccaga acccggcgct gatttccgcc gcactaagtt
    16141 tgtggcagca gggcaaaatc gccccggaca gctgggtgat cgacgtggat cagatactgg
    16201 aaaacggtaa gcggctcatt gagacggcgc ggctttacgg cattgaactg tatctgatga
    16261 ccaagcaatt tggtcgtaat ccgtggctgg cggaaaaatt gctggcatta ggctacagcg
    16321 gcattgtggc ggtggattac aaagaggcgc gagtcatgcg ccgcgctggt ttgccagtgg
    16381 cgcatcaggg gcatctggta caaatccctt gtcatcaggt tgctgacgcc gttgaacagg
    16441 gcaccgacat catcaccgtg tttactctcg acaaagcgcg ggaagtttct gcggcagcgg
    16501 ttaaggccgg acgggtccag cctgtgctgc tcaaagttta tagcgacgat gattttcttt
    16561 atccggggca ggagagcggt tttgtacagc attcgctgca cgaggtgatt gctgaaatta
    16621 agaaactgcc ggggctgcat ttagccggac taacccattt cccttgcctg ctttgggatg
    16681 aggctgctgg gaaagttttg ccaacaccga atcttcacac gctggtacaa gcacgggatc
    16741 aactggcgaa atctggtatt gcgattgagc aactgaacgt accttcagcg accagctgca
    16801 cttcgctgcc attgctggcg gaatacggtg cgactcatgc cgaacccggt catgcgctga
    16861 ccggtactat tccggcaaac cagcagggcg atcagcctga acgtatcgcg atgctctggt
    16921 taagtgaaat ctcccatcat ttccgtggcg acagctactg ctacggcggc ggttactacc
    16981 gtcgtggtca tgcgcaacat gcgctggtgt ttacgccaga aaatcaaaag attactgaaa
    17041 ccaatctcaa aactgtggat gacagcagta tcgactacac cctgccgctg gcaggcgagt
    17101 atccggtgag cagtgcagtg gtgctctgtt ttcgcacgca gatttttgtc acccgtagcg
    17161 atgtggtgct ggtgtccggt attcagcgcg gcgaaccaga aatagttggt cgttatgaca
    17221 gcctcggcaa ccctctggag gcataaatgg cgcgatttgt ggtgttagtg attgatagct
    17281 ttggcgtagg ggcaatgaaa gatgtcacgc tggtgcgccc gcaagatgcc ggggcgaata
    17341 cctgtggtca catcctgagc cagttgccgc atttgcagct accaacgctg gagaagctgg
    17401 ggctaatcaa cacattgggt tatgcgccag gcgatatgca gccgtcagat tccgcaacct
    17461 ggggcgtggc agagctgcaa catgaaggtg gcgatacctt tatggggcat caggaaattt
    17521 taggcacgcg cccgttaccg ccgctgcgga tgccttttcg cgatgtgatt gaccgtgttg
    17581 agcaggcatt agtttccgct ggctggcagg tggagcgccg tggcgatgat ctgcaatttc
    17641 tgtcggtcaa tcaggcggtt gcgattggcg ataatctcga ggcggattta ggccaagtct
    17701 ataacattac cgccaatctc tctgtgatct cttttgacga cgcaatcaaa attggtcgta
    17761 tcgtgcgtga ccaggtacag gtcggtcggg tcattacatt tggcggcctg ttaaccaaca
    17821 gtcagcgtat tctcgatgcc gcagaaagca aagaagggcg ctttattggt attaatgcgc
    17881 cgcgttctgg cgcttatgac aacggcttcc aggtcgtgca tatgggctac ggtgttgatg
    17941 aaaaagtgca ggtgccgcaa aaactgtatg aagcgggcgt gccaaccgtg ctggtgggta
    18001 aggtggcaga tatcgtcagc aatccttatg gtgtgagctg gcaaaacctg gtggatagcc
    18061 agcggattat ggatatcacc ctcgacgaat ttaacaccca tccgacggcg tttatttgca
    18121 tcaatatcca ggaaaccgac ctcgctggtc atgcagaaga cgtcgcacgt tatgccgaac
    18181 gtttgcaggt cgttgaccgt aaccttgccc ggcttgttga ggcgatgcag ccagatgatt
    18241 gcctggtcgt gatggcggat cacggtaacg atccgaccat tggtcacagc caccataccc
    18301 gtgaagtggt gccagtgctg gtttatcagc aagggctggt cgccacgcag ctcggtgtgc
    18361 gcaccacgct ttctgatgtg ggagctaccg tgtgtgaatt tttccgtgcg ccaccgccac
    18421 aaaatggtcg ctcttttctt tcctccctcc ggtttgcagg agacacccta tgagttttga
    18481 tccgacgggt tacaccctgg cccatgagca tctgcatatt gatctctccg gctttaaaaa
    18541 caacgtggac tgccgccttg atcagtatgc gttcatttgc caggagatga acgacctgat
    18601 gacccggggt gtacgtaatg tgattgagat gaccaaccgt tacatggggc gcaatgcgca
    18661 atttatgctc gatgtaatgc gcgaaaccgg gatcaacgtg gtggcctgta ctggttatta
    18721 ccaggatgcg tttttcccgg aacatgtggt gacccgcagc gtgcaggaac tggcgcagga
    18781 gatggtcgat gaaattgaac agggtatcga tggcacggag ctgaaagccg ggatcatcgc
    18841 ggagatcggc tccagcgaag gaaagattac gccgctggaa gagaaggtat ttattgccgc
    18901 tgcgctggcg cataaccaga ccggacgccc gatctccacg catacgtcgt tcagcaccat
    18961 ggggctggaa caactggctc tgttgcaagc ccacggagtt gatctttcgc gcgtcaccgt
    19021 tggtcactgc gatctgaaag acaacctcga caacattttg aagatgatcg atctcggcgc
    19081 gtacgtgcag ttcgacacca ttggtaagaa cagttactac ccggacgaaa agcgcattgc
    19141 gatgctccac gcgctacgtg accgtgggtt gctgaaccgc gtcatgctgt cgatggatat
    19201 tacgcgccgc tcccatttaa aagccaatgg tggttatggc tatgactatt tattgaccac
    19261 ctttattccg caattgcgcc agtcaggatt cagtcaggcc gatgtggatg tgatgttacg
    19321 tgaaaatccc tctcaatttt tccaataagg acagactcat gaaaaagatt ggcgttgcag
    19381 gcttacagcg tgagcagatt aaaaaaacca ttgaagcgac ggctcctggc tgttttgaag
    19441 ttttcattca caacgacatg gaagcggcaa tgaaggtgaa atccgggcaa ctagattatt
    19501 acattggcgc gtgtaatacc ggtgcgggcg cggcattgtc gattgccatc gcggtgattg
    19561 gctataacaa aagttgcacc attgccaaac caggcattaa agcgaaagac gagcatatcg
    19621 ccaaaatgat cgctgaaggg aaagtggcgt ttggcctttc cgttgagcac gtcgaacacg
    19681 cgattccgat gttgattaac catctgaaat aaaaggcacg actatggatc tgtatattca
    19741 gattatcgtg gtggcgtgcc tgacgggtat gacatcgctt ctggcgcatc gctcggcggc
    19801 tgtttttcat gacggcatcc acccgatcct gccgcaactg attgaaggct atatgaaccg
    19861 tcgcgaggcg gggagtatcg cttttggtct gagcattggt tttgtggcct cggtggggat
    19921 ctcttttacc ctgaaaaccg ggctgctcaa cgcatggtta ctctttcttc ctaccgatat
    19981 cctcggcgta ctggcgataa acagcctgat ggcgtttggt cttggcgcta tctggggcgt
    20041 gttgatcctt acttgcctgt tgccagttaa ccagctgctg accgcgctgc cggtggatgt
    20101 attaggtagc ctcggggaat taagctcgcc ggtggtttct gcttttgcac tgttcccgct
    20161 ggtggcgatt ttctaccagt ttggctggaa gcaaagtctg gtcgccgccg tggttgttct
    20221 gatgacccgt gtggtagtcg tgcgctattt cccacatctt aaccctgaat ccatcgaaat
    20281 ctttattggc atggtgatgc tgctggggat cgcgataact cacgacctgc gtcatcgtga
    20341 tgaaaatgac atcgatgcca gcgggctttc ggtgtttgaa gagcgcacgt cgcggattat
    20401 caaaaactta ccttatatcg ccatcgtggg agcattgatt gccgccgttg ccagtatgaa
    20461 gatttttgct ggtagcgaag tttcgatctt cacactggag aaagcgtatt ccgcaggcgt
    20521 gacgccggaa caatcgcaaa cgctgattaa tcaggcggct ctggcagaat ttatgcgcgg
    20581 actggggttt gtgccgttga ttgccaccac cgccttagca acgggtgtgt atgcagttgc
    20641 gggctttacc tttgtttatg cggtgggcta tctctcgccg aatccgatgg ttgcagcggt
    20701 attaggcgca gtggttattt cggcggaagt cttgctgctt cgttcgatcg gcaaatggct
    20761 gggacgctac ccgtcggtgc gtaatgcgtc ggataacatc cgtaacgcca tgaatatgct
    20821 gatggaagtg gcgctgctgg tcggttcgat tttcgcagca attaagatgg cgggttatac
    20881 cggattctct atcgcggttg ccatttactt cctcaacgaa tccctgggcc gtccggtaca
    20941 gaaaatggcg gcaccggtcg tggcagtaat gatcaccggt attctgctga atgttcttta
    21001 ctggcttggc ctgttcgttc cggcttaagg aaggcaccta tgaagacgtt tcctttgcaa
    21061 agcctgactc ttgctgaagc gcagcaaaag cagtttgcgc tggtggacac tatttgccgc
    21121 cattttccag gcagcgagtt tcttgctggc ggtgatttag ggttaactcc aagtctgaat
    21181 caaccgcgta ttacccaacg ggtggagcag gtgctggctg atgcatttca cgcacaggcc
    21241 gcggcgctgg tgcagggcgc ggggactggc gcgattcgcg ccgggctggc ggctttgctc
    21301 aaaccggggc agcgtcttct ggtgcatgac gcgcctgttt acccgacgac acgggttatt
    21361 attgagcaga tggggctgac gcttattact gctgatttca atgacctgtc ggcactgaag
    21421 caggtcgtcg acgagcaaca acctgatgcc gcgctggtgc agcatacgcg ccagcagccg
    21481 caggacagct atgtgctggc agatgtgctg gcaacgttgc gcgcggcagg tgttccagcg
    21541 ttaaccgatg ataactatgc ggtgatgaag gtagcgcgaa tcggctgtga atgcggcgcg
    21601 aatgtctcga cattctcctg cttcaagctg tttgggccgg agggcgttgg tgcagtggtt
    21661 ggcgatgccg atgttattag tcggattcgc gccacgcttt actccggcgg tagccagatc
    21721 cagggctcac aggcgctgga agtcttgcgt ggtctggtgt tcgcgccagt gatgcatgcg
    21781 gtgcaggcgg gagtatccga acggttgctg gctttgctta acggcggcgc ggtggcggaa
    21841 gtgaaaagcg ccgtcattgc caatgcgcag tcgaaggtgt tgattgtcga gtttcatcag
    21901 ctgattgccg ccagggtgct ggaagaagcg caaaagcgtg gcgcgttgcc ttacccggtc
    21961 ggtgcagagt cgaaatatga aatcccgccg ctcttctatc gactttcagg aacgtttcgc
    22021 caggcgaatc cacaactcga acattgtgcg attcgcatta acccgaatcg cagcggtgaa
    22081 gagacggtgc tgcggatttt gcgtgagagt attgcgagta tttaatgctg gatagcaccg
    22141 ttcggcaatc aagctaaatt ccgcactgaa cggtcccctc gcccctttgg ggagagggtt
    22201 agggtgaggg gaacgtgcgg gatgtaaaat tcagtgcgcc agcatcgccg gatgcgatgc
    22261 tggcgcatct tatccggcct acggttaatg tcttttatta tcaatcgtta aggtgatgcg
    22321 attggcgcgg cagaagagtt cggagacgtg caccggtttg tcattctgat caaaggcgat
    22381 tttgctgatg cgaaacaacg gttcgcccag ttcgcatttc agccattttg cctgcgggcg
    22441 ggtggcggca aagatatcga tggtcttttt gtcgctgacc actcgcgtgt caaaacgctc
    22501 ctgaaataac tgataggtgg agcttccttc gacgtaaatc tcatcaaagt caggataacg
    22561 cgacagcggg atccatgaac tatcaataaa cagcggctct ttatcgagat acatcacccg
    22621 gcagagatgg aacacttcgc tgtttccggg gatgttcagc ttttcgcaaa acggcgcggc
    22681 gctgacccgt tcctgttcga tcactttctc tttcgtcgac ttgccttgtg agacgccaaa
    22741 atcggt
//
LOCUS       DAJTMX010000060        22555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTMX010000060 DAJTMX010000000
VERSION     DAJTMX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.060"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="OO145_002275"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="OO145_002275"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5467378.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="OO145_002276"
     CDS             691..1317
                     /locus_tag="OO145_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5467379.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="OO145_002277"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="OO145_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="OO145_002278"
     CDS             3452..4090
                     /locus_tag="OO145_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5467380.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="OO145_002279"
     CDS             4154..4822
                     /locus_tag="OO145_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5467381.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /locus_tag="OO145_002280"
     CDS             4819..5604
                     /locus_tag="OO145_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5467382.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /locus_tag="OO145_002281"
     CDS             5608..6420
                     /locus_tag="OO145_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467383.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..7851)
                     /locus_tag="OO145_002282"
                     /pseudo
     CDS             complement(6432..7851)
                     /locus_tag="OO145_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7977..8282)
                     /locus_tag="OO145_002283"
     CDS             complement(7977..8282)
                     /locus_tag="OO145_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5467384.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8390..8466)
                     /locus_tag="OO145_002284"
     tRNA            complement(8390..8466)
                     /locus_tag="OO145_002284"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8430..8432),aa:Val,seq:gac)
     gene            complement(8471..8547)
                     /locus_tag="OO145_002285"
     tRNA            complement(8471..8547)
                     /locus_tag="OO145_002285"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8511..8513),aa:Val,seq:gac)
     gene            8855..10111
                     /locus_tag="OO145_002286"
     CDS             8855..10111
                     /locus_tag="OO145_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5467385.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10152..11525)
                     /gene="mdtK"
                     /locus_tag="OO145_002287"
     CDS             complement(10152..11525)
                     /gene="mdtK"
                     /locus_tag="OO145_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5467386.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11740..12381
                     /gene="ribE"
                     /locus_tag="OO145_002288"
     CDS             11740..12381
                     /gene="ribE"
                     /locus_tag="OO145_002288"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5467387.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12421..13569)
                     /gene="cfa"
                     /locus_tag="OO145_002289"
     CDS             complement(12421..13569)
                     /gene="cfa"
                     /locus_tag="OO145_002289"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5467388.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13860..15071)
                     /locus_tag="OO145_002290"
     CDS             complement(13860..15071)
                     /locus_tag="OO145_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5467389.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15184..16116
                     /locus_tag="OO145_002291"
     CDS             15184..16116
                     /locus_tag="OO145_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5467390.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16113..17138)
                     /gene="purR"
                     /locus_tag="OO145_002292"
     CDS             complement(16113..17138)
                     /gene="purR"
                     /locus_tag="OO145_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5467391.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17437..17526
                     /gene="ynhF"
                     /locus_tag="OO145_002293"
     CDS             17437..17526
                     /gene="ynhF"
                     /locus_tag="OO145_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5467392.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17692..18861
                     /locus_tag="OO145_002294"
     CDS             17692..18861
                     /locus_tag="OO145_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467393.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19007..19588)
                     /gene="sodB"
                     /locus_tag="OO145_002295"
     CDS             complement(19007..19588)
                     /gene="sodB"
                     /locus_tag="OO145_002295"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5467394.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19716..20543)
                     /gene="mepH"
                     /locus_tag="OO145_002296"
     CDS             complement(19716..20543)
                     /gene="mepH"
                     /locus_tag="OO145_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5467395.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20878..21225
                     /gene="grxD"
                     /locus_tag="OO145_002297"
     CDS             20878..21225
                     /gene="grxD"
                     /locus_tag="OO145_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5467396.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21264..21764)
                     /gene="tnpA"
                     /locus_tag="OO145_002298"
     CDS             complement(21264..21764)
                     /gene="tnpA"
                     /locus_tag="OO145_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5467397.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21772..>22555
                     /locus_tag="OO145_002299"
     CDS             21772..>22555
                     /locus_tag="OO145_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5467398.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNH"
BASE COUNT         5861 a         5250 c         5832 g         5612 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taagtgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgcttgaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 cctggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcagatc cccgcttcac gtaaggccag
     6661 tagtcgacga atagactctg aagggattgc cgcataatta tcgatgaaaa ccctggcaag
     6721 gcctttactg aaccgtttat ggtcgtgttc attcagatga ggaacaattt cctgtacggc
     6781 ttcatgcagg cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg
     6841 ttcgacttcc tggagatttt tttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc
     6901 aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca
     6961 gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc
     7021 ctcttcacct ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt gtaagggctc
     7081 gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg acatcaacgt
     7141 aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt tatcatcttc
     7201 aatgaacgaa ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt
     7261 tcccataata cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct
     7321 gggaaaatac gttctggttg cttcttcatc aggccagacg tgacccgtgg caatcaccgc
     7381 taaatcaaac gtctcgctgg gtaaatcctg atttgtagcg agcatgacgc cagcatttgt
     7441 aatttgcaga tcggtaacct ggcatgattc ataaacagcc actgcaaatt tttgctgtcg
     7501 tgcctggtct actaatcgta aaaattgatc gcggaaatat tcgcccagca gaattcgcgg
     7561 taaaaactga cgatcgtgca aggtttcttt tttaacgcca taacgctgga gatggctggc
     7621 ttcttgcttt tgtagccatt cgagatacgt acaattaatc ggcggtattt caatactggc
     7681 aatatttgcc agcatcattt ttgagttttc ctcatcactg tatggcatcc cgacaccggc
     7741 ctcgtcagcc tgctcgaaga tagaaataga aagtggagtt tgttgctgta gaagcgagaa
     7801 taaggtgtag atccccgtag gcccggcacc cacaatagca atttttttca ttgttactta
     7861 cctgtacgca ttctttcgaa tttagtgtta tcagataagt ttggttgaga tttttagaat
     7921 ttgagaaatt tcagacaggg atgaaaaggg cgctaaccgc ccttgattaa attctgtcag
     7981 gcgagttttg cctgattgat ttgactaagt ggctcattga catcgaaaac tttggcgaca
     8041 acttcctcaa cgccgagatt tttcagtcgg gcagtatgtt tttccagata ggcaagggcg
     8101 cttttttcat cagtgaaaag gtagatccca ccggcttcgt ggttcttttc actttctgtc
     8161 cataccttcc acagaaaacc aggttcctga ttaatcgact cagcaagtgg tttaagctgc
     8221 tcagccattg cgtcgccaaa tgggccatta aaagcaaaat gaagttgtaa caaagttgcc
     8281 atagcgattc tccttctgat aacgcatacg aagacagtgt aaatctttta acgtattgga
     8341 tacagagaag aaacagaggg aaaaaggaaa agaccagatt aagaaaatct ggtgcgtccg
     8401 agtggactcg aaccaccgac ccccaccatg tcaaggtggt gctctaacca actgagctac
     8461 ggacgcagga tggtgcgttc aattggactc gaaccaacga cccccaccat gtcaaggtgg
     8521 tgctctaacc aactgagcta tgaacgcaac gttgtaggtg acaacgggga cgaatattag
     8581 cggcagagtg gaaaggtggc aagaggcaaa acgtaatttt ctgcgctatt tcgaccgttt
     8641 gcagagcttt taagcaaatt ggctatattt tgttgatttg caagggcgat ttttattcag
     8701 gatcgcattt acatctgata caacccaata attcgtgtat cagagtttaa acagagttgt
     8761 ttgccaccat ctcaaatctg tcgtggataa atcctccgta gcctttacac ttaggtagca
     8821 ataaaggcac acagatgcag caaaggaata aatcatggga tctgatgcga aaaacttgat
     8881 gagcgacggg aatgtgcaaa ttgttaagac cggcgaggtc cttggcgcga cgcaacttac
     8941 tgaaggcgag ttgattgttg aagctggcgg aagagccgaa aataccgtgg tcacgggggc
     9001 tggctggttg aaagtggcaa ccggtgggat cgccaaatgc acacagtacg gtaacaatgg
     9061 cacgctatcg gtcagcgacg gtgccattgc cacagatatt gttcagtccg agggaggcgc
     9121 aattagtctc tctacgctcg ctacggttaa tggccgccat cccgaaggtg aattcagcgt
     9181 tgatcagggt tatgcctgcg gtttgttgct ggaaaatggc ggtaacctgc gtgtactgga
     9241 aggacatcgc gcggaaaaaa tcattctcga tcaagagggc ggcctgttgg ttaatgggac
     9301 aacctcagcg gtcgtggtag atgaaggtgg tgaattgttg gtgtatccaa gtggagaagc
     9361 cagcaattgt gagattaatc agggcggcgt ttttatgctg gccgggaaag ccagtgatac
     9421 gttgcttgct ggtggcacca tgaataatct cggtggtgaa gactctgaca ctattgttga
     9481 gaatggatcc atttatcgtc tggggacgga tggccttcag ctctacagtt ccggtaagac
     9541 gcaaaacctg tcggtgaatg tgggggggcg ggctgaagtg catgccggta cgctggaaaa
     9601 tgcggtaata caaggtggaa cagtgatcct gttgtcaccc accagcgcgg acgaaaattt
     9661 tgtcgtagag gaagatcgcg caccggttga actgaccggg agtgttgcat tactggacgg
     9721 cgcttcaatg attattggtt atggcgcaga gctgcaacaa tcaacgatta ctgtacagca
     9781 gggcggtgtg ttgattctcg acggcagtac ggtaaaaggt gacagcgtca ctttcattgt
     9841 tggtaacatc aatctgaatg gcggaaaact gtggctgatc actggtgcgg caacgcatgt
     9901 gcaactgaaa gtgaaacgcc tgcgcggaga gggagcgatt tgcctgcaaa ccagtgcgaa
     9961 agaaatttca cctgacttca tcaatgtgaa aggggaagtt aacggggata tacgcgttga
    10021 gataacagat gccagtcggc aaactctgtg taacgctctg aaattacagc cagacgaaga
    10081 cgggattggc gcaacgctcc agcctgcgta aaaacacagg cgcaacccgt gaaggttgcg
    10141 ccttgtcttt attagcggga cgctcgttgc agaatgatgg ctgacggcat acgttgcagg
    10201 aaccgcatac gcagcatcat cataatggct gccgatgtca ggccgataat aaagcctatc
    10261 cagaagcctg ctggccccat aggttcaacg accagatcgg tcagtgccag aatatagccg
    10321 cttggcaagc ccagcaccca gtaagctgta aaggtgatat agaaaatgga acgcgtatct
    10381 ttataaccac gcaaaatccc actgccaatc acctggattg agtcagaaat ctgatatacc
    10441 gccgccagca acatcaaatg cgcagccagc gttacaacct cgggattgtc gttgtacaac
    10501 agggcgattt gctcccgcag tgaaaccgtg aaaatggccg tcagggttgc catacagaca
    10561 cccaccataa gcccggtccg cgcagcggtt tgcgcatcca gcgttgagcc ctgacccaga
    10621 cgataaccta cgcggatagt taccgctgcc gccagcgaca ttggaagcac aaacatcagt
    10681 gaactaaagt tcagggcaat ctggtgtcct gcgacatcaa caataccgag cggagacact
    10741 aacagagcca cgacggcaaa cagtgtcact tcaaagaaca gtgccagcgc aatcggcaaa
    10801 ccgagttgaa tcagtcgttt cataaccgca ggctcgggct tagcggtgcc tttttcgtta
    10861 cgaatatcgc gcatggagcg ggcgcgttta atgtaagaaa ccatggcaag gaacatgacc
    10921 caatacaccg ccgcagtagc cacgccacaa ccaacgccac cgagctcagg cataccgaaa
    10981 tgaccataaa taaagatata gttcaccggg atgttcacca gcaggccgat aaagcccatt
    11041 accatacccg gcttggtttt tgccagacct tcacattggt tacgggcaac ctggaagaac
    11101 agatatcccg gcgcgcccca caacaacgca cgcagataac ccacggcttt gtccgccaga
    11161 gccggatcga tgttttccat gtagcggata atgtaacctg cattccacag caccagcata
    11221 atgagaacgg aaacaaaacc tgccagccag aaaccttgtc gcacctgatg cgcaatgcgc
    11281 tcacgtcgac cggaaccatt taattgcgcg ataaccggcg ttaatgccag cagcagtccg
    11341 tgaccaaaga ggatcgccgg aagccagata gaagtaccga tagcgaccgc cgccatgtcg
    11401 gtggcactat agccgcccgc catcacggta tcgacaaaac ccatcgcagt ttgggcgatt
    11461 tgcgcgagaa tcaccgggat tgctaatgct aataacagac gcgcttcact gatatacttc
    11521 tgcacgtgaa caccttttat ttgtagttat atgagagact aaaaagccgc ctgaatgggc
    11581 agcaaaaaga agaagcaggg gaaattcagt ctattgtagc gaggatttat taattctcca
    11641 gtgaaaaaat agccattctt accccttcac tggtaagggc gttttccacc tgctattgtg
    11701 ctgaacagaa tgtcttaact gatttcagga gttgtaagta tgtttacggg gattgtacag
    11761 ggcaccgcaa aactggtgtc gattgacgag aaaccaaatt ttcgtacgca tgtggtggag
    11821 ttacccgacc acatgctgga cggcctggaa accggtgctt ccgtggcgca taacggttgc
    11881 tgcctgaccg tgacggaaat taacggcaac catgtcagtt ttgacctgat gaaagaaacg
    11941 ttacgcatta ccaatcttgg cgatttaaaa gtgggggatt gggtaaacgt tgagcgtgcg
    12001 gcgaaattca gtgatgaaat tggcggacac ttaatgtcag gtcatattat gaccaccgct
    12061 gaagtggcga aaatattaac ctcggaaaat aatcgccaga tctggtttaa agtccaggat
    12121 agtcagttga tgaaatatat cctgtacaaa ggatttattg gcatcgacgg tattagcctg
    12181 accgtcggcg aagtcacgcc aacgcgtttt tgcgtccatt taattccgga aacactggaa
    12241 cgcacgactc ttgggaagaa aaaacttggc gcacgcgtca acattgaaat cgatccacaa
    12301 actcaggcag tggtagatac ggtagaacgt gtgctggcgg cacgagaaaa tgccatgaat
    12361 caaccaggta cagaagcctg atctgaactc gcccccgaag agggggcgat agaatagcct
    12421 ttagcgagcg actcgaaggc cgttttcaac accgcgtgag aacaccacct gccagagctg
    12481 aatatcacgg gcgcggaaag cacctgcgca ggcattcaga taataggtaa acatccgttt
    12541 aaagcgttca ctgtagttat ccgcaatttc tggccatgcg gcgaggaatc gttcatacca
    12601 cgccatcaac gtagtatcgt aatcagcacc gaagttatgc cagtcttcca tcacaaagtg
    12661 gggttcgctg gattgagcaa tctggcgtac agagggcagg caaccgttcg gaaaaatata
    12721 tttattaatc cagggatcaa cattcagatc ggtttttttc gaaccgatag tatggagcag
    12781 gaatatacct tccggtttca aattacgatc caccaccgca aaataggtat cgtaattttt
    12841 cggtccgacg tgctcgaaca tccccacaga aacaatacga tcaaactggt cgttcaggtc
    12901 acgataatct tgcagcaaaa tggtgacatc caggccttta cagcgttcct gagccatttt
    12961 ttgctgttcg gcagaaatgg tgacgccgac cacgcttacg tcatagttag atgccatgta
    13021 gtgcgctagt ccgccccagc cgcagccaat atccagtacg cgcatccctg gttttaactg
    13081 caatttttca caaatcattt tgagcttcgc ctgctgggca gattccagat tatcggcatc
    13141 tttccagtaa gcgcaggaat attgcatgaa gggatcaagc atgcggctga acaagtcatt
    13201 acccaaatcg taatgctcct tgccgactat ccaggcacgt tttttactct gcagattgaa
    13261 gagacgagcg ccggcaatac gcagcgtgtc tttgaaatga tgggggagtt ggttctcgag
    13321 acctgcgcgt aagactttgc taaaaaacat atccagtcgg tcacattccc accagccatc
    13381 catataactt tcgcctaacc ccaaagagcc ttcctgcaga acgcgtttaa aaaaatcggg
    13441 gtttttcaca cgaatatccg ccggggcaga accgttaatg gctataccgg cacggctaag
    13501 taattcgttg gcgatacggt accagttgtc atccggtaca ctgacttctt ctatacacga
    13561 tgaactcata gtttctccat cactggtcgg tgatcagaac cttaaaacag cgtagacgct
    13621 ttttgggctt tgtgagaaat ctcgcagaaa aaaccgcgag tctataatcc gacgtagaac
    13681 agagggaaag gaggaaccct tgccgaaatg accatccata gtgagtcggg aacgatcctg
    13741 tccccgtaaa aacataatat ttatcgtagc tttaacaact cgaaattagt ataggccgct
    13801 aatttcaatc atacaacaaa attttgttag cagcctaagt ataagtatat cgatatagat
    13861 cagtgtgatt cgctatgagc gacttcggca ttgccatgat tcgggcagtc aacttcttca
    13921 caacgttgca tcatgtaacc cagcgcgacc agcactactg ttgataacat cacgctggtg
    13981 gtggtgagca atggcgtgct gatactaata agccaggaaa ctaccagact tgcgaggaag
    14041 cacagaccca gttgcagagt gttctgcaac gctgcggcgc gaccggttgc atgtgggaag
    14101 ggacgcagcg cctgggcgac aacaataggg tagatcgcgc cattggcaat cgccatcaca
    14161 cagaatggga tcaggatttc gaccagcgac acatggctaa tgaagcccgc agcccaggtc
    14221 gcaatgacgc tgacagcaaa cagcaccagc aaccacggta ataactgctt gccttgccat
    14281 ttctgcagtg cggcgcgaca gccataacca ccaatcagaa acgcgatagt ttgcgggaca
    14341 taacttaaac caataactgc cgggctgtag cccatttcac taaggatgaa cggtgaaccg
    14401 gtcagccatg caaaaaaact ggctgaacag gctgcgtata tcagcacgtt gccgcgatag
    14461 gttttagaac gtagcaggtc ggtaaaggtc agaccatcct gactattgtt acgggccttc
    14521 gtcgtgggtt tgagccagaa aataggcaga atcagcacca cggtaatggc aaacagggtg
    14581 gcgaaaatcg cctgccagga aaaatggacc agcagccagc ttcctaacag gggagccagt
    14641 gccggagaca gacccaccag cggcatgatg gtcgcaaaaa tacggttgac tttctgtgaa
    14701 ggataataat ctgtcactaa tgcttgccag ataaccgccg cggcgcagac ccccacagcc
    14761 tgtacaaaac gcaataccag cagcgtggcg gcgttttcta cccacagcat ccccagacta
    14821 cctaacgcaa aaattgtcag gccgattaat aacaccggtt tacgaccata acggtcggag
    14881 agtggccccc acagaagctg ggctgcggca aaaccggcaa ggaacagact aaggctggca
    14941 ctgacagcag acgcaggcgt ttgcaggtcg gcctgtatgg cggcgaaagc aggcagatac
    15001 atatcggttg ccagaaaacc gagtacgctc aaacccgcca gccagactaa aaatcttttc
    15061 ccaggttgca tgtatattct ctaaatctct caattaaaca ccgccgcaaa gtgtagggag
    15121 tgaaattagt gttgtgaaac ggtaatattt gatgactggt ttcaaaaatt ttgcaggcag
    15181 actatgtggt cagaatattc actcgaagtt gttgatgcgg tagcgcgtaa tggtagtttt
    15241 agcgctgcgg cacaggagct gcatcgtgtt ccttctgcgg tcagctatac cgtgcgtcag
    15301 ctggaagagt ggctggcggt accgctcttt gaacgacggc accgtgatgt ggagctgacc
    15361 gctgctggag cgtggtttct caaagaaggg cgctctgttg tcaaaaaaat gcagatcacc
    15421 cgccagcaat gtcagcagat agcgaacggc tggcgcggtc agttagctat cgcagtggat
    15481 aatattgtca ggccagaacg tacacggcag atgatcgttg atttttatcg ccattttgat
    15541 gatgtcgaac ttcttgtctt tcaggaagtg ttcaacggtg tctgggatgc gctttccgac
    15601 gggcgcgtgg aactggcaat tggcgcaaca cgcgcgattc cggtaggcgg tcgttatgcc
    15661 ttccgggata tggggatgct aagctggagt tgcgttgttg ccagtcacca cccgctggcg
    15721 ttgatggatg gcccgttcag cgatgatacg ttgcgcaact ggccgtcgtt ggtgcgcgaa
    15781 gacacctcgc gaacgctacc taaacgtatt acctggttgc tggataacca aaaaagactt
    15841 gtcgtacctg actgggaatc atcggcaacc tgtatctcgg caggattatg catagggatg
    15901 gtgccaacac atttcgccaa accgtggctt aatgagggga agtgggtagc acttgaactg
    15961 gaaaacccct tcccggattc ggcatgttgt ctgacatggc agcaaaatga tatgtcgcct
    16021 gcgttaacct ggctgcttga gtatctcagc gatagtgaga cgctgaataa ggagtggctg
    16081 cgggagccgg aagagactcc cgcaacgggt gattaacgac gatagtcgcg gaacgggccg
    16141 tcagccacgg agcggcgttc aatcaaacgc ggatgcactt caatggactg cggttcttca
    16201 cgtttgttga cgatacgatc caacagcatg ttgaacgctg tttcacccag cgaatctttt
    16261 ggctgatgga tcgtggtcag cgccggcgta aaatagcgcg cgttgcgcac gttatcataa
    16321 ccgatcagcg aaacatcctg cgggacgcgc aggcccattt catcagcagc acaaagtgcg
    16381 cccattgcca tgatatcgcc accacagaag acggcagtag ggcgatgcgg ctgcgacagg
    16441 atttgctgca tggcgcgata accggattca ggttcaaagt caccctgcac aatccagctt
    16501 tccggcacct tgatcatcgc ttcttccatc gccttcataa aaccggcaag gcggcctgcg
    16561 ccggtgttac gttccagcgg gccggggatg acgccgattt cgcggtgacc gcgttcaatc
    16621 agataacgcc cggccatgta gccgccttcg aacgcgttat caatgaccgc atcggtgaag
    16681 tcagcttttg cttcacccca gtccatcacc accattggga tatggcgata ctcttccagc
    16741 atcgccagca acggctctgg gtactcagaa cacatcacca gcagaccatc gacgcgtttt
    16801 tgcgccatca tcgacagata agcccgctgt ttctcaagat tgttccacgc attacccaga
    16861 atcagggtgt aacctttctg gaagcaattt ttttcaactg cttcaatgat ctcggcaaaa
    16921 taggccgctt cgctgctggt cgccagcaaa ccgatagact tggtgtggtt aaccttcagg
    16981 ctacgcgcca ccgcgctagg ggagtagtgt aattctttaa tcgctgccca cacggcgttg
    17041 cgcgtttctt cagcgacgaa acgtgttttg ttgatcacgt gtgacacagt tgtagtggaa
    17101 acgtttgctc gtttcgctac atcttttatt gttgccattc catttcactc cagaccctaa
    17161 cttcagctcc tgcaaaatcg caaggtaaac gtttgccttt acacacctta cgcacaacaa
    17221 aagtgttgcg gtacgccgga aaaacgacac ggagcgtcag gaaggggtca atggccagta
    17281 cgcaaatagt gtaagcgtgg aattttggcc gatcttgacg aaaaggggaa gtggtaaaaa
    17341 cacttatcat tataaatgac gggagatttt ttcatcacag tgtgtaaaaa tgcgtaatat
    17401 cactttttgt gtggtaataa aaaaggagaa aacttgatga gcaccgacct taaattttca
    17461 ctggtaacaa cggttatcgt cctcggtttg atcgtagccg tgggtttgac tgccgcgctg
    17521 cactgatttc ctctcgaggg agcgcgttct ccctcgcatc tccttcctgt ttgttagcaa
    17581 aatggcaaaa gtgttttctc tttttgttat tttcattttt ttgtgattgc tgtcatgctt
    17641 tcgccacttt aatgaatatc gcgtaatgac gcgaagaggt ggagataagg catgaaaatt
    17701 aactatccgt tgctggcgct ggcgattggc gcgtttggta tcgggacaac ggagttctcg
    17761 ccaatgggct tgttgcccgt cattgcgcgc ggtgtggatg tctcgattcc cgctgccgga
    17821 atgttaatca gtgcctatgc agttggcgta atggtgggcg cgccgctgat gacgcttcta
    17881 ctttctcatc gtgcccgccg cagtgcgttg attttcctga tggcaatttt cacgctaggc
    17941 aacgttcttt ccgccatcgc gccggattat atgaccctga tgctttcacg tattttgacc
    18001 agcctgaatc acggagcatt ttttggtttg ggttcagtcg tggccgcaag cgtggtgcca
    18061 aaacataaac aggccagcgc agttgccact atgtttatgg ggttaaccct ggcaaatatc
    18121 ggtggcgtgc cggcggcgac ctggttgggt gaaaccatcg gctggcggat gtcatttctg
    18181 gcaacggcgg ggctgggagt gatttcaatg gtaagtctgt tcttctcatt acctaaaggt
    18241 ggtacagggg cacgacctga agtgaaaaaa gagctggcgg tattaatgcg tccgcaggtg
    18301 ctgtctgcat tgctgacgac ggtactggga gctggtgcaa tgtttaccct ctacacctat
    18361 atctctccgg tactgcaaag tattacccac gcaacaccgg tgttcgtcac ggcaatgctg
    18421 gtgctgattg gtgtcggatt ctctatcggt aactatctcg gcggcaaact ggcagatcgt
    18481 tcagttaacg gcacgttgaa aggctttttg ttgctgctga tggtgattat gctggcaatc
    18541 ccgttcctgg cccgcaatga gttcggcgca gctattagca tggtggtgtg gggcgcagca
    18601 acctttgcgg ttgtacctcc gttacagatg cgcgtgatgc atgtcgccag tgaagcgcca
    18661 ggtctgtctt catcagtcaa tattggtgcc tttaatcttg gaaatgcgct gggagcagct
    18721 gctggtggtg cggtaatttc cgctgggctg ggatacagct ttgtgccggt gacgggggcg
    18781 attgtcgcgg gactggcatt attgctggtg tttatgtcag ccagaaaaca acctgaaaca
    18841 gtttgcgttg ctaacagcta attaaacaaa aacgcagaag gataaccttc tgcgttacag
    18901 ttgctgacaa cgtgcgattt atcttgccga atgcgctgtt aacgcctcat ccgcgttggc
    18961 atggtaagcg aagcgcatca ggcaatgctg catttgccat cagttattat gcagcgagat
    19021 ttttcgctac gaattcccag ttcaccagcg cccagaagtg ctccagatag ccaggacgtg
    19081 cattgcgata gtcgatgtaa taagcgtgtt cccagacatc aacggtcagc agcggagtcg
    19141 catcggtggt cagcggagta cccgcgttag aggttgaaac gatagccagt ttgccatcgc
    19201 tgtttttcac cagccaggtc cagccagaac caaagttttt gatcgctgca tcagtaaact
    19261 gcgctttgaa atcggcaaag ctgccaaaag atgcggcgat agcttcagcg acttttccag
    19321 tcggttcgcc accggcgttc ggtgccaggc agttccagta gaaagtatgg ttccagacct
    19381 gagctgcgtt gttgaatacg ccaccttcag agctgcgaat aatctcttcc agtgatttac
    19441 cttcaaacgc ggtaccttta atcaggttgt tcaggttagt gacataagtc tgatgatgct
    19501 tgccgtagtg atactcgata gtttccgcag aaatgtgcgg tgccagagca tctttagcat
    19561 atggtagtgc aggtaattcg aatgacattg ctactctcct ttattattaa tttgcatacg
    19621 tacaatagcc ttattgtgcg tatggatagg gtagcaaatt aaaaggtgag acaaaagatg
    19681 aacttaccct gttgccgtaa caacagggta aagttttagc gaagtgtttt tggggtcatt
    19741 acccgacgag cgccaacata gtggcgctgc cagtagtctt cactgagaga agtgatttga
    19801 atttcctgac ctgtgcgcgg tgactgaata aatttgccgt tgccgacata cacgccgaca
    19861 tgatcggctg taccgcgtcc ctgagtacgg aaaaagacca ggtcgccgtt tttcagctca
    19921 ctacgttcga taggtcctgc atcacgcagg tgatacattt cattcgccgt acgcggaata
    19981 cgaattttca ccaaatcttt ataagcgtaa taaaccaggc cgctgcaatc aaaaccggta
    20041 cgcggtgagc tgccgcccca acgatatggc ttaccaattt gctgcatcag tttattcatt
    20101 gccactttcg tggctttttg caccttcgct ttgtgcgcgt cggcaagggt tcctgacgcg
    20161 gcatttttga ctttcacaca atgcgattta tagcccttac gtttggtaca cttttcggta
    20221 aagctggctg aggccgtttt attgacggcg tgtttgctgc gagttttggc gtttttagaa
    20281 gcggtaatgg aagatttttt aacagaagaa gcggttttgc ttttggtcat acttttactg
    20341 gcggcttttt tcgctgtttt ctggctggct ttctgggtct ttttggtggt tgccgtgctt
    20401 ttctttttat ctgctttttt ggtgatatga gtggtagcag aactctccct ggcttgcttt
    20461 gaagcatggg ccataggcgt taaaggcagg gtggtaaaaa gtaaagcaca gagcgtgatc
    20521 gaaatacggt ttatccgcgc cactgagcgt ccctctgtta aggcaggtct atcatgcctg
    20581 cgtctgattt acaaaacttg atgattctaa tgcatagaag aagagaaagt taatagcttt
    20641 tattgttaaa aaatacgttt cgttggcgag cgcaaaaaaa tgcagcagtg aaagctaaaa
    20701 atggttataa atacgccagt ttgccgcgca tcagagaaga aacgaatatt tgaaatgcaa
    20761 caaatagagc aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg
    20821 cactgacccg ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg
    20881 agcaccacta tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg
    20941 aaaggttcac cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc
    21001 gcatgtggcg aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa
    21061 ctgccgaaat atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc
    21121 ggcggttgtg atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa
    21181 gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac
    21241 tgacggatgc gaacgggcat ccgtcagtcc tttatccggt gcgccgtaaa acctcgtcct
    21301 tcagggcggg gatataaggc gcggttttcc acctgaccag gtgtttgctg ttgttcaatg
    21361 tactggcgga tgacggatat tggcgcacct tcgcagctac tggcaaaata gccaggactc
    21421 cacaaaacgc ctttgtagta atacctgact gcaatatctg gtcgatctcg tcgcagtaac
    21481 ctattcgata tgcctttgag gctgtttacc agactggata tcgccagttt gggaggatag
    21541 ttaattaaca aatggacgtg atctggttcg ccatccattt caaccagttc agctgcaaaa
    21601 tcagcacata catttgaaaa gtaagtgcgt agtttttctg tcgtgtcatg gtcaaaaatc
    21661 tggcgtcggt atttggtgac aaagaccagg tgaacatgca tcaggaaaac acaatgcctt
    21721 ccacgccgga tatcggtttc ttttttcaca gaccaaagta tcatttgacc tgtgaaacga
    21781 ttacaggcat ttaaattcca gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc
    21841 ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg cgcttcagaa tgagaatcat
    21901 gaggccggta ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa
    21961 aacgccactg aaacgcaatg gcttaaagat tcgccctcac agccattgca acagtcactg
    22021 aaagaccttg agcgggctta caaaaacttc ttccagaagc gggctgcttt tccccgattc
    22081 aaaaagcggg gacagaatgc tgcattccgc tacccgcagg acgttaagct cgatcaggaa
    22141 aacagccgta tttttctgcc gaaacttggc tggatgcgct accggaatag ccgtcaggtc
    22201 acgggagttg tgaaaaatgt cactgtcagc cagtcctgcg gtacgtggta catcagtatt
    22261 cagacagaaa gtgaagtatc aactccggtt cacccttcag catcaatgat cgggctggat
    22321 gctggcgtgg ctaaactcgc cacgctgtca gatggcacag tctttgagcc tgtaaacagt
    22381 tttcaaaaaa accagaagac gctggcgaga cttcagcgac agttaagccg gaaggtcaaa
    22441 ttcagcaaca actggcagaa gcagaaacgc aaaatacagc gactgcattc ctgtatcgca
    22501 aatatccgca gggactacct tcacaaagtc acaacgaccg tcagcaaaaa ccacg
//
LOCUS       DAJTMX010000061        22392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTMX010000061 DAJTMX010000000
VERSION     DAJTMX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.061"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO145_002300"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO145_002300"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5467399.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO145_002301"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO145_002301"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5467400.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO145_002302"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO145_002302"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5467401.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO145_002303"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO145_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5467402.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO145_002304"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO145_002304"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5467403.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO145_002305"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO145_002305"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5467404.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO145_002306"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO145_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5467405.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO145_002307"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO145_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5467406.1"
                     /translation="MTTLTLVLTAVGAVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO145_002308"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO145_002308"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5467407.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO145_002309"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO145_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5467408.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO145_002310"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO145_002310"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5467409.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO145_002311"
     CDS             complement(15665..16702)
                     /locus_tag="OO145_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5467410.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO145_002312"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO145_002312"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5467411.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO145_002313"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO145_002313"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5467412.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO145_002314"
     CDS             19444..19884
                     /locus_tag="OO145_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5467413.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO145_002315"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO145_002315"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5467414.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO145_002316"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO145_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..>22392)
                     /gene="ugpE"
                     /locus_tag="OO145_002317"
     CDS             complement(21603..>22392)
                     /gene="ugpE"
                     /locus_tag="OO145_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5467415.1"
                     /translation="IAVILFPLYVAFVAATLDKQAVYAAPMTLIPGTHLLENIHNIWV
                     NGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAIVWFRFPLRNLFFWMIFITL
                     MLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATFLFRQFFMTLPDELVEAARI
                     DGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWPLLIITDVDLGTTVAGIKGM
                     IATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGLVDSEK"
BASE COUNT         5408 a         6219 c         5607 g         5158 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gcccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgctgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tc
//
LOCUS       DAJTMX010000062        22278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTMX010000062 DAJTMX010000000
VERSION     DAJTMX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.062"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1025)
                     /locus_tag="OO145_002318"
     CDS             complement(<1..1025)
                     /locus_tag="OO145_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467416.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="OO145_002319"
     CDS             complement(1127..2107)
                     /locus_tag="OO145_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5467417.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="OO145_002320"
     CDS             complement(2115..2825)
                     /locus_tag="OO145_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5467418.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO145_002321"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO145_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5467419.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="OO145_002322"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="OO145_002322"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5467420.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="OO145_002323"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="OO145_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5467421.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="OO145_002324"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="OO145_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5467422.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="OO145_002325"
     CDS             9402..9512
                     /locus_tag="OO145_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5467423.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO145_002326"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO145_002326"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5467424.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO145_002327"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO145_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5467425.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12394..13026
                     /locus_tag="OO145_002328"
     CDS             12394..13026
                     /locus_tag="OO145_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5467426.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO145_002329"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO145_002329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5467427.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="OO145_002330"
     CDS             16558..17490
                     /locus_tag="OO145_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5467428.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="OO145_002331"
     CDS             complement(17531..18961)
                     /locus_tag="OO145_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5467429.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKNRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO145_002332"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO145_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5467430.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO145_002333"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO145_002333"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5467431.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="OO145_002334"
     CDS             complement(20822..21091)
                     /locus_tag="OO145_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5467432.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="OO145_002335"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="OO145_002335"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5467433.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="OO145_002336"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="OO145_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5467434.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5513 a         5584 c         5332 g         5849 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctgttttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgc
//
LOCUS       DAJTMX010000063        22222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTMX010000063 DAJTMX010000000
VERSION     DAJTMX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.063"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1086)
                     /gene="alaS"
                     /locus_tag="OO145_002337"
     CDS             complement(<1..1086)
                     /gene="alaS"
                     /locus_tag="OO145_002337"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5467435.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKT"
     gene            complement(1214..1714)
                     /gene="recX"
                     /locus_tag="OO145_002338"
     CDS             complement(1214..1714)
                     /gene="recX"
                     /locus_tag="OO145_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5467436.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(1957..3018)
                     /gene="recA"
                     /locus_tag="OO145_002339"
     CDS             complement(1957..3018)
                     /gene="recA"
                     /locus_tag="OO145_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5467437.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(3098..3595)
                     /gene="pncC"
                     /locus_tag="OO145_002340"
     CDS             complement(3098..3595)
                     /gene="pncC"
                     /locus_tag="OO145_002340"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5467438.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(3740..4825)
                     /gene="mltB"
                     /locus_tag="OO145_002341"
     CDS             complement(3740..4825)
                     /gene="mltB"
                     /locus_tag="OO145_002341"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5467439.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            5081..5643
                     /gene="srlA"
                     /locus_tag="OO145_002342"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             5081..5643
                     /gene="srlA"
                     /locus_tag="OO145_002342"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            5640..6599
                     /gene="srlE"
                     /locus_tag="OO145_002343"
                     /gene_synonym="gutE"
     CDS             5640..6599
                     /gene="srlE"
                     /locus_tag="OO145_002343"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5467440.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            6610..6981
                     /gene="srlB"
                     /locus_tag="OO145_002344"
                     /gene_synonym="gutB"
     CDS             6610..6981
                     /gene="srlB"
                     /locus_tag="OO145_002344"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5467441.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            6985..7764
                     /gene="srlD"
                     /locus_tag="OO145_002345"
     CDS             6985..7764
                     /gene="srlD"
                     /locus_tag="OO145_002345"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5467442.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            7869..8228
                     /gene="gutM"
                     /locus_tag="OO145_002346"
     CDS             7869..8228
                     /gene="gutM"
                     /locus_tag="OO145_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5467443.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            8295..9068
                     /gene="srlR"
                     /locus_tag="OO145_002347"
                     /gene_synonym="gutR"
     CDS             8295..9068
                     /gene="srlR"
                     /locus_tag="OO145_002347"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5467444.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            9061..10026
                     /gene="gutQ"
                     /locus_tag="OO145_002348"
     CDS             9061..10026
                     /gene="gutQ"
                     /locus_tag="OO145_002348"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5467445.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(10023..11537)
                     /gene="norR"
                     /locus_tag="OO145_002349"
     CDS             complement(10023..11537)
                     /gene="norR"
                     /locus_tag="OO145_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5467446.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            11724..13163
                     /gene="norV"
                     /locus_tag="OO145_002350"
     CDS             11724..13163
                     /gene="norV"
                     /locus_tag="OO145_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5467447.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            13160..14293
                     /gene="norW"
                     /locus_tag="OO145_002351"
     CDS             13160..14293
                     /gene="norW"
                     /locus_tag="OO145_002351"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5467448.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(14421..16673)
                     /gene="hypF"
                     /locus_tag="OO145_002352"
     CDS             complement(14421..16673)
                     /gene="hypF"
                     /locus_tag="OO145_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5467449.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(16826..17353)
                     /gene="hydN"
                     /locus_tag="OO145_002353"
     CDS             complement(16826..17353)
                     /gene="hydN"
                     /locus_tag="OO145_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5467450.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(17502..18512)
                     /gene="ascG"
                     /locus_tag="OO145_002354"
     CDS             complement(17502..18512)
                     /gene="ascG"
                     /locus_tag="OO145_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5467451.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(18526..18645)
                     /locus_tag="OO145_002355"
     CDS             complement(18526..18645)
                     /locus_tag="OO145_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5467452.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(18655..20364)
                     /gene="hycE"
                     /locus_tag="OO145_002356"
     CDS             complement(18655..20364)
                     /gene="hycE"
                     /locus_tag="OO145_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5467453.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20382..21305)
                     /gene="hycD"
                     /locus_tag="OO145_002357"
     CDS             complement(20382..21305)
                     /gene="hycD"
                     /locus_tag="OO145_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5467454.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21308..>22222)
                     /locus_tag="OO145_002358"
     CDS             complement(21308..>22222)
                     /locus_tag="OO145_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5467455.1"
                     /translation="AGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWF
                     RTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGA
                     FVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVA
                     LAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICK
                     GDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQ
                     CEGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5216 a         6250 c         5778 g         4978 t
ORIGIN      
        1 agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc
       61 cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg cgccgagcat
      121 attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac ggttttcatt
      181 ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac
      241 gcgcagcgat ttattgctca gatcggtcgc gccagtgact ttcgctaccg cctggatcaa
      301 cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg cagcaatacg
      361 ctccagaccc ataccggtat ctacagacgg cttcggcagc ggttccatcg tgccatctgc
      421 ctggcggttg aactgcatga agacgatgtt ccagatctca atgtagcggt cgccgtcttc
      481 ttccggactt cccggagggc caccccaaat gtggtcgccg tgatcgtaga agatttcggt
      541 gcacgggccg cacggaccag tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc
      601 acctttgtta tcgccgatgc gaataatacg ttcgcgcggg atcccgactt ctttttccca
      661 gatttcgtag gcttcgtcgt cgctttcata gacggtaacc cacagacgct ctttcggcag
      721 ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa
      781 atagtcgccg aagctgaagt tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta
      841 accgacgttt tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt
      901 agcgcgggaa taattacgct tgtcgagccc aaggaacaca tccttgaact ggttcatccc
      961 ggcgttggta aacaacaaag ttgggtcgtt atggggtacc agggagctgc tggcaactac
     1021 ctgatgtccc ttactatgga aaaagtcgag aaacgcctga cggatctcag cggtgctctt
     1081 gctcataatt atcctgaaat caagctaacg aaatatcgcc accagctcca gcgtgtctta
     1141 accgccgggc tggtaactga aaagtgggaa taagataagt tttcttgact gggaagtaaa
     1201 ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc ctggatatct tccatcagat
     1261 agccacgata gagcagaaaa cgctggatct taactttttc tgaaaagaca gttggcaaag
     1321 gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt
     1381 cacgcatcgc tttttctgtc gcttcgcggg aaataccttt ctgattcagt tcctgacgaa
     1441 tacgtgcagg tccataccct ttgcggctac ggctggcgat aaagcgcgca acaaatcggc
     1501 tgtcatcgag atagccatgt tcatggcacc aggcaataac gcgctcgtaa tcttctgccg
     1561 tagcatcaat ctcttctggg ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca
     1621 gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag
     1681 catatgccgg gcgacgggat gttgattctg tcatggcata tccttacaac ttaaaaaagc
     1741 aaaagggccg cagatgcgcc ccaggttttg ctgataaaca gatttaatag tcaatgagta
     1801 tgcagaatgt tcccgcaggc ccaattcgtt agaggatcga ggtgagagta atttttcacg
     1861 ccagtgctgg cctgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc
     1921 gtgcacacat tatctattat catcaataac gaacgattaa aaatcttcgt tagtttctgc
     1981 tactccttcg ctgtcatcta cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa
     2041 ctcacgtact ttcttctcga tctctttcgc ggtttccggg ttatctttca gccaggcagt
     2101 tgcattcgct ttaccctgac cgatcttctc acctttgtag ctgtaccacg cgcctgcttt
     2161 ctcgatcagc ttctctttta cgcccaggtc aaccagttcg ccgtagaagt tgataccttc
     2221 gccgtagagg atctgaaatt cagcctgttt aaacggtgca gcgattttgt tcttcaccac
     2281 tttcacgcgg gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg
     2341 gatgtcgaga cgaacagagg cgtagaattt cagcgcgtta ccaccggtag tggtttccgg
     2401 gttaccgaac atcacaccaa ttttcatacg gatctggttg atgaagatca gcagcgtgtt
     2461 ggactgcttc aggttacccg ccagcttacg catcgcctgg ctcatcatac gtgccgcaag
     2521 gcccatgtga gagtcgccga tttcgccttc gatttccgct ttcggcgtca gtgccgccac
     2581 ggagtcaacg acgataacgt ctactgcgcc agaacgcgcc agggcgtcac agatttccag
     2641 tgcctgctcg ccggtatccg gctgggagca cagcaggtta tcgatatcga cgcccagttt
     2701 acgtgcgtag attgggtcca gcgcgtgttc agcatcgata aacgcacagg ttttaccttc
     2761 acgctgcgct gcggcgatca cctgcagcgt cagcgtggtt ttaccggaag attccggtcc
     2821 gtagatttcg acgatacggc ccatcggcag accacctgcc ccaagcgcga tatccagtga
     2881 aagcgaaccg gtagagatgg tttccacatc catggaacgg tcttcaccca ggcgcatgat
     2941 ggagccttta ccaaattgtt tctcaatctg gcccagtgct gccgccaacg ctttctgttt
     3001 gttttcgtcg atagccattt ttactcctgt catgccgggt aataccggat agtcaatatg
     3061 ttctgttgaa acaattatac tgtatgctca tacagtatca agtgttttgt agaaattgtt
     3121 gccacaaggt ctgcaatgca tacgcagtag cctgacgacg caccgcatca cggtcgccgc
     3181 tgaagcattc ccgccgggta atgccttcac cgcgggcagt ggcaaaagca aaccagacgg
     3241 tgccgacagg cttctcttca ctgccgccat ccggcccggc gataccacta atagacacgg
     3301 cataatcagc acgagccgct ttcagtgcac ctatcgccat ttccaccacg acgggttcac
     3361 tcaccgcgcc atgctgcgcc agcgtctctt cgcgtacgcc gatcatctgc gctttggctt
     3421 cgttactgta ggtgacaaat ccgcgttcaa accaggcgga gctaccggca atatcggtaa
     3481 tcactttcgc tacccaacca ccggtacaag actcggcagt tgttacggtt gcgccacggg
     3541 ctttcagcgc ctgcccaacc tgttcgctta actgcatcag ttcactgtca gtcatcacaa
     3601 cctctgtcag tcaaaaattt atgccggaca agatagcact ttcgtcagga agatggtgtt
     3661 ggcgttgcaa ttttacgagg aggattcaga aaaaagctga ttagccagag ggaagctcac
     3721 gcccccctct tgtaaatagc tactgtactc gcgccagcgc cacggcttgt cctaactgcc
     3781 agaccgccat tgcgtaatgg gtgctgtggt tgtaacgggt gatggtgtag aagttcggca
     3841 gaccgtacca gtactggtag ccggtgccaa catccagacg cagcaggctg gcttgttgat
     3901 ggttgcccag cggctgctgt ggcgttaaac ctgcggcggc aagctgcgaa atgctgtact
     3961 tagttttgaa accatttggc aagcctggag cctgaccgtt tgccattatc gcgacctgat
     4021 cgcctttcac ccagccgtgc gctttgaaat agttcgccac gctaccgatc gcatcaaccg
     4081 gatcccacag gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt ttgtaagacg
     4141 acggcataaa ctgtccgtag cccatcgccc cggcaaagga acctttcaga ttgagcggat
     4201 cgtcctgctc gtcgcgcgcc atcagcagga aggtttccag ctcgccagag aaatactccg
     4261 cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc gaggatgcga gttttcccca
     4321 tcacgcgccc ccagcgggtt tcaacgccga taatcccgac gataatttcc ggcggtacgc
     4381 catacacctg ccacgcgcga ttcaacgcat cttcatactg attccagaaa accacaccgt
     4441 tctgcacgtt gtccggcgta ataaattttt tgcgataacg cagccatgcg ccgttcggac
     4501 ctgatggcgg tttcaccgat gtggttggtg cctggttatc catcagccgc agtaccgaat
     4561 ccagacgctt cgcctgggag agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt
     4621 tcaccatttt gtcgatgaac tgctgggcat tcgggttatt agcgaaatcg ccgcccatct
     4681 gcatcacatt gtgctgcggc tcaagcagga agccgccaga cggcgttccg gtggtcgttt
     4741 cagtctcagt aggttttggc ttgctgctac aggcggcaag caacacaaaa aggggaagca
     4801 atgttacata acgacgcttg aacatgaggg gtccatttaa cagattcaac caggggcaag
     4861 tatggtaaag catcacaccc cgcacaagga agcggtagtc actgcccgat acggacttta
     4921 cataactcaa ctcattcccc tcgctatact tttattcaaa ctttcaaatt aaaatattta
     4981 tctttcattt tacgatcaaa ataacacttt taaacctttc aacctgatta gattaggttg
     5041 ccgtttggta ataaaacaat aaatcctgaa ggagagaaca atgatagaaa ccattactca
     5101 tggtgcagag tggtttatcg ggctgttcca aaagggcgga gaggtgttta ccgggatggt
     5161 gaccggcatt cttccgctgt tgattagcct gcaggttatc atgaacgcac tgattaattt
     5221 tatcggtcag catcgtattg aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg
     5281 ttacctactg ttaccgtgca ttggcacgtt tgtcttttgc aatccgatga ccttaagcct
     5341 gggtcgcttt atgccggaaa agtacaaacc cagctactac gcggcggcct cttatagctg
     5401 ccactcaatg aatggcctct tcccccatat caaccctgcg aactgtttgt ttatcttggc
     5461 attgccagcg gtctgacaac gctgaacctg ccacttggcc cactggcggt gagttatctg
     5521 ctggttggtc tggtcaccaa tttcttccgc ggctgggtga ccgatctgac caccgccatt
     5581 tttgagaaaa agatgggcat tcaacttgaa caaaaagttc acctggcagg agcaacatca
     5641 tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg cggcccgctt gagctggaag
     5701 ccacgccggg caaaaaaatc gtctatatca ccgccggtac ccggcctgcg attgttgaca
     5761 aactggcaca acttactggc tggcaggcta ttgacggatt taaagaaggt gaacccgcgg
     5821 aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac attacgctgc ggcatctatc
     5881 cgaaacgacg tattcccacc attaatatcc actcgacggg caagtccggt ccgctggcgc
     5941 agtacattgt ggaagatatt tatgtctctg gcgtaaaaga agaaaacatc actgtagtgg
     6001 gtgatgcgac accacaaccc tcttccgtgg gccgtgacta tgacaccagt aagaaaatca
     6061 ccgaacaaag cgatggttta ctggcgaagg tgggaatggg catggggtcc gccgttgccg
     6121 tgttgtttca gtctggtcgt gacaccatcg acactgtatt aaaaaccatt ctgccgttta
     6181 tggcattcgt ctcggcgctc attggcatca ttatggcttc tggccttggt gactggattg
     6241 cccacggtct tgctccgcta gcgagccatc cactgggtct ggtcatgctg gcgctcatct
     6301 gctccttccc actgctttca cctttcctcg gcccaggcgc agttatcgca caggttatcg
     6361 gcgtattgat tggcgtgcag attggtctcg gcaatattcc gccgcatctg gctttaccgg
     6421 cactgtttgc catcaacgcg caggcggctt gcgacttcat cccggtcggt ttgtcgctgg
     6481 cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc tgtactggtg agccgctttt
     6541 taaccggcgc gccgactgta ctgatcgcct ggtttgtctc cggttttatc tatcaataga
     6601 ggctgaaaca tgaccgttat ttatcagacc accatcaccc gtatcggcgc gagtgccact
     6661 gacgccctca gcgaccagat gctcatcacc tttcgtgaag gcgcgccagc ggacctcgaa
     6721 gagtattgct tcattcattg ccacggcgag ttgaaaggtg cactccatcc cggtttgcaa
     6781 ttttcactcg ggcagcatcg ctatccggtg accgctgttg gcagcgtggc ggaagacaac
     6841 cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt taaacgaagc ggaatttccg
     6901 ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg cgccgggatc ggttttgaag
     6961 tttgaatctg ttaaggagta aaaaatgaat caggttgccg ttgtcatcgg tggtgggcaa
     7021 accttaggcg cgttcctgtg ccacggtctg gctgccgagg ggtatcgcgt cgcggttgtc
     7081 gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa ttaacgccga atatggtgaa
     7141 ggtatggcgt acggttttgg tgctgacgcc actagcgagc aaagcgttct ggcgctctct
     7201 cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg tctatagcgc cggaatagca
     7261 aaagcagcct ttatcagcga cttccagctc ggcgattttg accgttcgct acaagtgaat
     7321 ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt tgatgatccg cgacgggatt
     7381 cagggtcgca ttattcagat caactcgaaa tccggcaaag tgggcagcaa acacaactct
     7441 ggctacagcg cagcgaaatt tggtggcgtc gggctgactc aatcactggc gctggatctg
     7501 gcggagtacg gcattacggt gcattcactg atgctcggta acctgctgaa atcgcggatg
     7561 ttccagtcac tgttgccaca atacgcgacc aagctgggta tcaaaccgga tcaagtcgag
     7621 cagtattaca tcgacaaagt accgctcaaa cgcggctgcg attatcaaga tgtgctgaat
     7681 atgctgctgt tctacgccag tcctaaggcg tcgtactgca ccggacagtc gatcaatgtc
     7741 accggcggtc aggtgatgtt ctgatcaaca gcggagatcc gttaaggatc tccgtgagac
     7801 tatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca ctacattaag
     7861 gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt gcgcaactgg
     7921 ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta tgccagcaag
     7981 ggcgggttgg cgtggaccgt tccagcgggc gctttaaacc gcgggtcgtg gtcgccatcg
     8041 cgctggacga tcagcagtgc atcgtcgaca ccttgtttat gaaaggactg actgtcttcg
     8101 cccgaccgca aaaaattccc gcaattaccg gcatgcatgc gggtgattta cagcccgatg
     8161 tgatctttcc ccatgatcca ctatcacaga atgctctatc attggcgctt aaactgaaac
     8221 gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga aacctaaatc
     8281 aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc cattctggag tatctgcaaa
     8341 agcagggtaa atgctcggtt gaagaattgg cgcaatactt tgacaccaca ggcacaacca
     8401 ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt cattcgtact tatggcggag
     8461 tggtgttgaa taaagaggaa tccgatccgc ctatcgatca taaaacgctc atcaacaccc
     8521 acaagaaaga gctgattgca gaagctgccg ttagttttat ccatgatggc gattcgatca
     8581 ttcttgatgc tggcagtacc gttttgcaga ttgttcccct gctctcgcgc tttaataaca
     8641 tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct atccgaactg gataacgaac
     8701 aaactatcct gatgccaggc ggaacatttc gcaaaaaatc ggcctcattt cacgggcaac
     8761 tggcagagaa tgccttcgag catttcacct tcgataaatt gtttatgggc accgacggca
     8821 tcgatctcaa tgcgggcgta accacgttca acgaggttta taccgtcagt aaggcaatgt
     8881 gcaatgccgc gcgcgaagtg attttgatgg cggactcatc aaagtttggc cgtaaaagcc
     8941 ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat taccgacgca ggtatcgatc
     9001 cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt gatcataacc ggagagagca
     9061 atgagtgaag cactactgaa cacgggacgt cagacgttaa tgctggagtt gcaggaagca
     9121 agccgtttac cggaacgtct gggcgatgat tttgttcgcg ccgccaatat catcctgcac
     9181 tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg taagaaaatc
     9241 gccgcaacac ttgccagtac cggcacaccg gctttttttg tccatccggc agaagcactg
     9301 cacggcgatc tggggatgat tgaaagccgc gatgtgatgc tgtttatctc ttactccggt
     9361 ggcgcgaagg aactggatct gattattccg cgtctggaag ataaatctat cgcgctgctg
     9421 gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg cgaaagcggt gctggatatc
     9481 tccgtagaac gcgaagcctg cccgatgcac cttgcgccga cctccagcac cgtcaatacc
     9541 ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggttt taatgaagaa
     9601 gattttgccc gctcccaccc agctggggca ctgggcgctc gcttgctgaa taaagtgcat
     9661 catctgatgc gtcgtgatga cgctatccca caggtagcgt taaccgccag cgtgatggat
     9721 gcgatgctgg aactcagccg caccggtctg gggctggtgg cggtatgtga cgctcaacaa
     9781 caggtacaag gcgtctttac cgacggcgat ttacgtcgct ggctggtagg cggcggcgca
     9841 ctcaccacgc cagttaatga agcgatgacg acaggcggca ccacattaca ggcacaaagt
     9901 cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa tcactgccgc accagtggta
     9961 gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg atttctatca ggccgggatt
    10021 atttaatcct tcaatcccag acgtttcgcc agccgatgca ggttggcgac gtcagtttcc
    10081 agcattcgcg cacaggctgc ccagttgtga tgattttgtg ccagtgcctg gcgaatagtt
    10141 tcacgctgga acgcttctgt cgcttcacgc aggttttgct taacaacggg caccgccgcc
    10201 gcttctggcg gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc aagaatcact
    10261 tcatcgccgt tgcgggtggc tctcgccagc actaccgccc gatgaatagc atgttccagt
    10321 tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat ttcgcgcccc ggcacttaat
    10381 accacgcggg agagcccctg ccgcaaacga cactgctcgc agaaataccc cgccagcaga
    10441 atgacatcat cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac gctcaggcga
    10501 tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt cgcgtaagtc gcggttagtc
    10561 gccgccagca cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg ctgaatatcg
    10621 ccatactgca acaccctcag cagcttggcc tgcaatgcca acgacaactc gccgatctca
    10681 tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc cgctgcggtt actgatagcg
    10741 ccagtaaacg ctcctttcac atgcccgaac aactcacttt ccgccacact ttccggcagt
    10801 gcggcacagt tgagatagac cagcggattc accgcccgtg gcgaggcttc atgaatcgct
    10861 ttcgccacca gctccttacc ggttcccgtc tcaccgctga tcagaacgtt gagatcggac
    10921 gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc caggggacaa gccaatcatc
    10981 tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg gcagcatatt ctggctttcc
    11041 agttgttcaa tcagcaacgc attgcttaac gctcccgccg ccagcgcggc aatcagccgt
    11101 aactcttcgt cgctgaaaac atcgaactga tcgggctgca tcccgtcgag cgtcagtgcg
    11161 ccgatcaggt tttgtccgac aaacaatggc agaccaacgc aggcgtgaac cttcagactc
    11221 tcctgcccag gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc gggaaagcgc
    11281 accacatccc cggcgcgggc aatcgcttcc agccgtggat gcccttccag cgcaaagcgt
    11341 ctaccgagta catcctttgc cagaccgtcg atggcaagcg gaataaactg ccgcgaatcg
    11401 taacgtagca acgcagacgc atcgcactcc agcacctgac gtagcgtggt gatcaggcgc
    11461 tgaaaacgat cctgatgacc aatcccacgc tgcaattcga tggcgatatt cgccagcaca
    11521 tcaacggaaa aactcatctt tgcctcactg tcaatttgac tatagatatt gtcatatcga
    11581 ccatttgatt gatagtcatt ttgactactc attaatgagc ataattttat ttatagagta
    11641 aaaacaatca gataagaaac tggcacgcaa tctgcaatta gcaagacatc tttttagaac
    11701 acgctgaata aattgaggtt gctatgtcta ttgtggtgaa aaataacatt cattgggtgg
    11761 gtcaacgtga ctgggaagtg cgtgattttc acggcacgga atataaaacg ctgcgcggca
    11821 gcagctacaa cagctacctc atccgcgaag aaaaaaacgt gctgatcgac accgtcgacc
    11881 ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg gcggatatcg
    11941 attacatcgt gattaaccat gcagaagagg accacgccgg ggcgctgacc gaactgatgg
    12001 cgcaaattcc cgatacgccg atctactgta ctgccaacgc tatcgactcg ataaatggtc
    12061 atcaccatca tccggagtgg aattttaatg tggtgaaaac tggcgacacg ctggatatcg
    12121 gcaacggcaa acagctcatt tttgtcgaaa caccaatgct gcactggccg gacagcatga
    12181 tgacttacct gacaggcgac gcggtgctgt tcagtaacga tgctttcggt cagcactact
    12241 gcgacgagca tctgttcaac gatgaagtgg atcagacgga gcttttcgag cagtgccagc
    12301 gttactacgc caatatcctg acgccgttca gccgcctggt aacgccgaaa attaccgaga
    12361 tcctgggctt taacttacca gtcgatatga tagcgacctc ccacggcgtg gtctggcgcg
    12421 ataacccgac gcaaattgtc gagctgtacc tgaagtgggc cgctgattat caggaagaca
    12481 gaatcaccat tttctacgac accatgtcga ataacacccg catgatggct gacgctatcg
    12541 cccagggaat tgcggaaacc gacccacgcg tggcggtgaa aattttcaac gtcgcccgaa
    12601 gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg gtcggcactt
    12661 cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct ggtggaggag atgaccggat
    12721 tacgcttccg taacaaacgc gccagtgctt tcggatctca cggctggagc ggcggtgcga
    12781 tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctc agcctgaaag
    12841 ccaaatggcg accagaccag gacgctctgg agttatgccg tgaacacggt cgcgaaatcg
    12901 cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt gaatacggta gttaaagaag
    12961 aaacctctgc cacgacgacg gctgacctcg gcccacgaat gcagtgcagc gtctgccagt
    13021 ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga acgccgtgga
    13081 gtgaagtccc ggataacttc ctctgcccgg aatgctccct cggcaaagac gtctttgacg
    13141 aactagcatc ggaggcaaaa tgagcaacgg cattgtgatc atcggttcgg gcttcgccgc
    13201 ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact attccattaa ccctgattgc
    13261 cgccgacagc atagatgagt acaacaaacc cgacctcagc catgttatca gtcaggggca
    13321 acgtgccgac gaccttaccc gccagacggc gggtgaattt gccgggcagt ttaatctgcg
    13381 cctgtttccg cacacctggg tgacggatat cgatgccgaa gcccatgtgg tgaaaagcca
    13441 gaataatcag tggcaatacg acaagttagt gctggcaacc ggtgccagcg cctttgtccc
    13501 gcctgtgcct gggcgtgagt taatgctgac gttaaatagt cagcaagagt atcgcgcctg
    13561 tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc ggcggtggtt tgattggtag
    13621 cgaactggcg atggattttt gtcgtgcagg caaagcggtc acgctaatcg acaacgctgc
    13681 cagtattctg gcgtcattaa tgccaccaga agtaagcagc cgcttgcagc atcggttgac
    13741 ggagatgggc gttcatctgc tgttgaaatc tcagttgcag gggctggaaa aaacggattc
    13801 tggcattctg gcaacgctgg accaccagcg tagtatcgaa gtggatgcgg taattgccgc
    13861 caccggactg cgcccggaaa cagccctggc acgacgcgcc gggctgacga ttaatcgcgg
    13921 cgtttgcgtc gatagttatc tgcaaaccag taatgccgat atttacgcgc tgggcgattg
    13981 cgcggaaatt aacggtcagg tattgccgtt cctgcagccg attcaactta gcgggatggt
    14041 gctggcaaaa aatcttctcg gcaataacac gccgctgaaa ctaccggcga tgctggtgaa
    14101 aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa acccagcgtc aggatttacg
    14161 ctggcagatt aataccgaac gccagggaat ggtggcgcgc ggcgttgacg atgctgacca
    14221 gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag gcatttgtat tgttgaaaac
    14281 gttgccggtg taggtgggct actgtgccta aaatgtcgga tgcgacgctg gcgcgtctta
    14341 tccgacctac ggggacgcat gtgtaggccg gataaggcat ttacgccgca tccggcaagg
    14401 gtgtccaaat gcaacacgct ttatccgttc tggacttcac ccgctaacca acgcgccgca
    14461 gcaataaccc cctgccccag agacaaaccg ccatcacccg ccggtaaact ctgtggaaag
    14521 agcaatgtga aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata
    14581 accccgccgc taaataccag cgtagtgata ccacgcatcg tggcctgctc acgcatcaac
    14641 gcggcaaaac cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aaccagtgcc
    14701 tgccagttca gccactgctg ccagaaagtg gcgagatcca gttgattgtc cacccgcggc
    14761 atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga gcgcctccag agcacaagcc
    14821 gcttcacctt cataacttaa cgtggctggc gcacagccca gtgccgccgc cactgcatcg
    14881 aacaaccgcc cacacgatga tgccagcggc gcgttaattc cacgctcaat ggcccgcgcc
    14941 agtacgctcc agttttgctg ttgcacactt gccgtttcag ggtaattctg ccactccggc
    15001 acaaagcgca ggcactgcgc cagcaggttt cgccacggct gcttcgccgc caaatcgcca
    15061 cccggaagcg ccactgcagg caagccgccc aggtgctcac attcgcgata gttcacccgc
    15121 aggcactcgc cgccccacaa agcgccattc tcccccatac cgataccgtc gagcgtcaaa
    15181 gcaatgacat caccgccatc cagcggccac tgatgctctg ccagacacgc cgctgcatgg
    15241 gcatgatgat gcagtaccgt ttgcgtcggc agattcattt cgctcgccca ctggctggag
    15301 acatagcccg gatgcacatc atgcacaatg tattgcggag tgaaatcgta gatgttttgc
    15361 atcaggcgta acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga
    15421 tgctgactca acaccgcttg ttcaccgcgc accaggcaga aggtattttt cagatccgcg
    15481 ccaagacaca gcacaggcgg aacattttta aagcccggag gcaaaaccag cgcatccggc
    15541 acataccccc gcgaacggcg cagcatttcg ccgctttcgc gcaccactga atcatccatc
    15601 cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg caatgccctg caaatccgcc
    15661 agcgcctgtt cgttgctgat agctggtggt ttaccgctca ggttgccgga ggtcatcacc
    15721 agcgggcatt gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacatcacc
    15781 ccgacttcgt taaggccagg ggcgatatca tcacaaagct caggtacgta ttttttatcc
    15841 accagcacaa tcggcgcagc gggcgtggta agcaactggc gcgcagcgtc tggtaaactt
    15901 tctgccactg gcaacatgac tgccagcggt ttcgccgggc gatgtttgcg ctcccgcagt
    15961 gtcgccaccg cgttactgtt acgtgcatcg caggcaagat gaaatccgcc aatccctttg
    16021 atggcgacaa tgttgcccat ttttaactgt gcgatagctg cctgtaatgc cgcctcttgt
    16081 tctgcatgtt caccatgact tacccattca agatgcgggc cacactccgg gcaggccacc
    16141 ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact ctttgtcaca ggccggacat
    16201 agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag gcatcgcgcg aataatggtg
    16261 aaacgcgggc cgcagtgggt acagttgata aacggataac gataacgccg ctcgcctggg
    16321 gtattcattt cggcaaggca agcagggcaa gtggcggcat ccgggacaat ttgcgtattc
    16381 atggtgccgc cggtgctctg gcggatggtg aactcggtgg gcagttgtga ccagataaac
    16441 ggctcacgct cgacgctatc aatacgcgcc agtggcggac agtgctgatg caattgaaca
    16501 agaaacgttt ccgggtcttc cagcagccgg acttctacgc catcgccgtc attacataca
    16561 tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa acggacgaaa accgacgccc
    16621 tgcactttgc cacgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta
    16681 ttcccgccat catgaattgc gtaacccgcc ctgccggaca cgacagcgtc gtatccggca
    16741 gtctcaggtc ggcgataccg cccgctccgt attctacgaa tatttccggg aattcctttg
    16801 atgccagaac agttctgtaa gatttttaga acatcagcgc cgtacggcgg cgtttttctg
    16861 cgctcagttg ttcaagttta ttacgatcga cacaaatcag cgcatgggtc gggcaagccg
    16921 ccatacacgc cgggccgtct tcacgatggt tgcacaggtc gcatttattg gcttcggctt
    16981 tgtcagcccg tacattcaga cccgcgccgc tgttgcgaat cacaggacgt accaccactt
    17041 ccatcgcgcc atacgggcaa gccaccacgc aggttttgca accaatgcaa cgttcctgca
    17101 tcacaggaac aaacccttta tcacggctga tagcaccatt cgggcagacg ttagcgcacg
    17161 gtgcatcttc acactgacgg caaactgtcg ccgtggaaat gttcacacct ttaatgacat
    17221 ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag
    17281 aaaccacgca cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga
    17341 tgaaacggtt catcaaattc tccagcaatg acagttaatg cgccgataca ctcacaaatc
    17401 atgccagttt ttaatttgct gttatttaag gaaattaatt tctgtaatgc aggaaaaaag
    17461 atgtcatcga cactagtgac gatgacatgt gatgacaatg tttatcgcga aagggcaatg
    17521 agtgagtcgc ggcggatcag ttttccgcta aaggttttcg gcggtgagaa atccccgcca
    17581 tcgagcataa aaatcagccg tccaataatt tcctgaatca tctcagtcac cggaattttt
    17641 acgctggaga gcgccggaac gatatagggg gcaattgcga tatcatcgaa tccgataact
    17701 gacacctgct ctggcaccgc tacgccgcgc tcgtgcaacg ctttgatcgc acctatcgcc
    17761 atatcgtcgt tactggcaac taacgcgcta aatttagccc ctcgttcgag caacgtttct
    17821 accccttcgg ccccgctggc gggcgtccat ttaccgttag cgataagttt ttcattgagc
    17881 gcaataccat gctgcgacag cgcgtcttta tacccggcaa gacgttcaat gctggtgggg
    17941 gaatccatcg agccggtaag gaaagcaatc tcctgatgcc cggcgtttat caactctgcc
    18001 acggcgttaa agctggtctg tttatgatcg caccagacgc tatggctgct attattgcgc
    18061 aggcggcgat taagcaccat tatcggctga ctgtgcgcgt caatgatgtc atcaatctca
    18121 tccacgctta aaaaacgcgg gtaaatcata atggcgtcgc agcgcaaatc cagcagatac
    18181 tgaatcgcct ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgtcgc
    18241 cctttctctt ccgccattcg cgcggcatga aacagtaatt cactaaaata aatgccgtga
    18301 taaagcgtgt tggtcactac cagccccagc gtctgagtac tcttcgccga cagattgcgg
    18361 gccagcaaat ttgggcggta actgctctct tctaccgcct gaaacacgcg atctttagtc
    18421 tcctggctga cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg
    18481 gcgcgcttcg ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac
    18541 gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc ggctccagcg gataagacga
    18601 ggtcgccgtg ccggttttga tgactttttt gataaaggta aacatggcga ttccttattt
    18661 cagcggcgag tttttacgct caatgctgta acgctcgagt tctttgtacg gcaccacttt
    18721 gctcttcttc ttacgcacat cgactacggt catgcggtcg gtacaggagt agcaagggtc
    18781 caggctaccg ataatcagcg gcgcatagga aacggtattg ccacgcagca tgtagcgcag
    18841 ggtcggccag ttcgcgtagg tcgcggcacg gcagcgccag cggtacagct tctggttgtc
    18901 gccggtcatg ctccagtgga tatcgtcgcc gcgcggcgct tcggcaaagc ccagcgcgaa
    18961 acggtgcgga atgtaggtaa agccttccac catcagcgga ccgcccggca ggttatccag
    19021 accgtagtcg atcatattca gcgcggtata gacctcgttg attcgcactt tcagacggga
    19081 aataacgtcg cagccctgct cgctgtggac ttccattggc agcaggccat aaccgacaaa
    19141 cgggtgatcg gcgcgagtat cgcgggcgag accgctggcg cgaaccatcg ggccaacgtt
    19201 actgaaatcg cgggcgattt ccgggtccag acgaccaatg ccgacggtgc gttgttccat
    19261 gttcggcgta ctcagcagca catccaccag ctcctgcact tcacgacgca tttgttgcgc
    19321 cagctggcgg gtctggatca tgtcatcttt cagcagatcg cgacgaatcc cgccgatcag
    19381 gttcaggccg taggttttac gcgcaccggt aaggatctct gccattttca tggaggtttc
    19441 acgcacgcgg aagaactgca taaagccgga gtcgaagccg gtaaagtgac aggcgaggcc
    19501 gaggttgagc agatgcgggt gcaggcgttc cacctccagc agaatggcgc ggatcatctg
    19561 cgcgcgttct ggcacctgaa tacccatcgc gttttccacc gacgtggtgt aggcggtgct
    19621 gtgggcaaag ccgcagatcc cgcacacacg gtcagagagg aaggtcactt cgttataacc
    19681 catgcgggtt tccgccagtt tttccatacc gcgatggacg tagaacagac ggtagtcggc
    19741 gtcgataatg ttttcgccat cgacgaacag acggaagtga cccggttcgt cagaagtgac
    19801 gtgcagcgga ccaatcggca cgacgttgtt tttcttgtcg cccagttcgt tgatgaactc
    19861 gtaggtttca gcatcggtag tcggtgccgg acgctgacga taatccatgc tgtctttacg
    19921 cagcggataa agttcatccg gccagtcatc cggcagcacc agacgacgtt catccggcag
    19981 accaaccgga atcaaaccgt acatatcgcg cacttcgcgc tcgccccaca ccgccgccgg
    20041 aacgcgcggc gtcacggacg gatactccgg tttgttggcg tcaacttcga cgcgaaccgt
    20101 tacccaacac ttggtgccct tctccatcga cagcacgtaa taaacggcgt aatgaccatt
    20161 cagtttgcgt tcgtcgttac caaacagcac cgacagccag ccaccctgct tgtagtagag
    20221 aaactccacc acttccggca gatagttcac ctttacggtg acagtcagct gatctttggt
    20281 ctgccaggcg tggtccagca cgacgcctag aaatgcctca ttcagcgcgg cgagataatg
    20341 ttgacctaat ttttcttcag acatgctcaa actctcttta atcacgccgc cagcaaggag
    20401 acgaacgcta aaaatgcaaa gccaaaccca gcccaggtaa tgcgcggagt aatatcaaga
    20461 cgcagacggg ccatgctgtt ttcgaacagc gcgataacca ggacgccgac caccagtttg
    20521 acgatggcaa tcaccagcgc cagcagcagt ccaccgacgg tgaaggtttc catttgtccc
    20581 cacggaataa acaccccgac gaacatctgc aacaccacca gctgtttcag gctgataccc
    20641 catttcatga cgccaaagcc gctaccgctg tattcggaga gcggaccttc ctgcaactcc
    20701 tgctcggctt ccgccaggtc gaacggcagt ttgcccattt cgataaaagt ggcgaacgca
    20761 caggcgcaga gcgccagcac cagcgggatg ctttggctca gcggccagtg ataaacggtg
    20821 tcggtaatgt tgctgatgtt ggtggaaccg gcaacctgtg cggcaaccca cagaccaagc
    20881 agcaacatcg gttcgaccag cacgccaagc atcgcttcac ggctcgcacc gatagcggta
    20941 aacgggctac cggtatccag accggaaatg gcaaagaaga aacgcgcgat ggcgaagaga
    21001 tacagtaagg tgatcaaatc gcccagttgc ggcagcggag aaccgacggt caccaccggc
    21061 agcgcagtag cgatagtcag catgacgccc accatcacat acggcgtcag gcggaacatc
    21121 cagccagagg catccgggcc aacgctctgg cggcccagca gtttgataat gtcgcgatac
    21181 tcctgcaaca cgcccggccc gcgacggtta tgcagacggg cgcgcgccac gcgggtaata
    21241 ccggagagca gcggcgcaac ggcaaataac accagcgcct gaattaacgg atataaaaca
    21301 ctcattctca ggctcctcgt gaaacaataa tcaccaccag taccgccagt tcaaccagcg
    21361 ccatccggcg gaacagcaac gcactacctt cgcactgcca gcccggcact agcgacaccg
    21421 gattcagcca tttgcgcagt ttcagcactg gcgcaaacgc ctgtttaacc ggcatggcaa
    21481 aaccgtgagc ggtaatcacc attgattttt catggtcgta gccgcacacc caggccgcac
    21541 cgcgggaacg tgacggcagg cggtcgcctt tgcaaatcgc cataatgatg aatggcagca
    21601 gcgggcaggc aatcagcagc aatgtgatca ttggttgaga aacggtggtg ttagcaggct
    21661 ccagaggcag aggtacagca gcagagagca tcggcagcag ccacggcgca gcaacaccgc
    21721 caattacgca gcaaatcgcc agtgccacta cgcttacgct catcaggagc ggcgcacagg
    21781 tggcgttttc ggcttctttg gtacgcggcg cgccgaggaa cgtgacgcca tagactttcg
    21841 ccatacacat caccgccagc gcaccggtaa ttgccagacc cacagcgagc agcggcccca
    21901 gcagacgggc aacaaacgcg ccgctattgc tcagtttgaa gaaggattga tagataaccc
    21961 attccccggc aaacccgttc agtggcggca gcgcagccat tgccatcaac ccgactaaca
    22021 tggcgatgga gataaccggc attttcttgc caataccacc gagtttttcg atatcgcgat
    22081 gaccggtacg gaaccagacg ctccccgccc cgaggaacag tacgcttttg aacaggctat
    22141 ggttaagcag atggtacaga ccaccgacca ggccaagagc aatcagcgcc ggttgttcga
    22201 gcgcgatacc cgttacgcca gc
//
LOCUS       DAJTMX010000064        21512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTMX010000064 DAJTMX010000000
VERSION     DAJTMX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.064"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO145_002359"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO145_002359"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5467456.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO145_002360"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO145_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5467457.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO145_002361"
     CDS             complement(2649..3200)
                     /locus_tag="OO145_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5467458.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO145_002362"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO145_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5467459.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO145_002363"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO145_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO145_002364"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO145_002364"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5467460.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO145_002365"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO145_002365"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5467461.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO145_002366"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO145_002366"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5467462.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQEMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO145_002367"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO145_002367"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5467463.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO145_002368"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO145_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5467464.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO145_002369"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO145_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5467465.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO145_002370"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO145_002370"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5467466.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO145_002371"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO145_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5467467.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO145_002372"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO145_002372"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5467468.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO145_002373"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO145_002373"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5467469.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO145_002374"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO145_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5467470.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO145_002375"
     CDS             complement(18170..18565)
                     /locus_tag="OO145_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5467471.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO145_002376"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO145_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5467472.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO145_002377"
     CDS             19481..20074
                     /locus_tag="OO145_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5467473.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO145_002378"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO145_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5467474.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="OO145_002379"
     CDS             20794..>21512
                     /locus_tag="OO145_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5467475.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5196 a         5323 c         5668 g         5325 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcatttctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAJTMX010000065        21503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTMX010000065 DAJTMX010000000
VERSION     DAJTMX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.065"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO145_002380"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO145_002380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5467476.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO145_002381"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO145_002381"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5467477.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO145_002382"
     CDS             complement(3121..3420)
                     /locus_tag="OO145_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5467478.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO145_002383"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO145_002383"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5467479.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO145_002384"
     CDS             complement(4632..5069)
                     /locus_tag="OO145_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5467480.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO145_002385"
     CDS             complement(5115..5456)
                     /locus_tag="OO145_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467481.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO145_002386"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO145_002386"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5467482.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO145_002387"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO145_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO145_002388"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO145_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5467483.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO145_002389"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO145_002389"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5467484.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO145_002390"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO145_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5467485.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPVSTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO145_002391"
     CDS             12687..13217
                     /locus_tag="OO145_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5467486.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO145_002392"
     CDS             13329..13865
                     /locus_tag="OO145_002392"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5467487.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO145_002393"
     CDS             complement(13973..14797)
                     /locus_tag="OO145_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5467488.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO145_002394"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO145_002394"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5467489.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO145_002395"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO145_002395"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5467490.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO145_002396"
     CDS             complement(18729..19202)
                     /locus_tag="OO145_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5467491.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO145_002397"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO145_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5467492.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OO145_002398"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OO145_002398"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5467493.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21503
                     /locus_tag="OO145_002399"
     CDS             21362..>21503
                     /locus_tag="OO145_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5467494.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5401 a         5490 c         5278 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggtctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgc
//
LOCUS       DAJTMX010000066        21275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTMX010000066 DAJTMX010000000
VERSION     DAJTMX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.066"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..444)
                     /locus_tag="OO145_002400"
     CDS             complement(<1..444)
                     /locus_tag="OO145_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5467495.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO145_002401"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO145_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5467496.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO145_002402"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO145_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5467497.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO145_002403"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO145_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5467498.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OO145_002404"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OO145_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5467499.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OO145_002405"
     CDS             complement(5214..6812)
                     /locus_tag="OO145_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5467500.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO145_002406"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO145_002406"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5467501.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OO145_002407"
     CDS             complement(8491..9069)
                     /locus_tag="OO145_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5467502.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO145_002408"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO145_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5467503.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OO145_002409"
     CDS             10508..10687
                     /locus_tag="OO145_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5467504.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OO145_002410"
     CDS             complement(10807..11166)
                     /locus_tag="OO145_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5467505.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OO145_002411"
     CDS             complement(11528..11872)
                     /locus_tag="OO145_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5467506.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OO145_002412"
     CDS             12004..13914
                     /locus_tag="OO145_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5467507.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OO145_002413"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OO145_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5467508.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OO145_002414"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OO145_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5467509.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="OO145_002415"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="OO145_002415"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5467510.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="OO145_002416"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="OO145_002416"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5467511.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO145_002417"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO145_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5467512.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO145_002418"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO145_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20410..20976)
                     /locus_tag="OO145_002419"
     CDS             complement(20410..20976)
                     /locus_tag="OO145_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5467513.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="OO145_002420"
     CDS             21188..>21275
                     /locus_tag="OO145_002420"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5467514.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5264 a         5273 c         5466 g         5272 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgtcta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caactttttg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgcccg cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAJTMX010000067        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTMX010000067 DAJTMX010000000
VERSION     DAJTMX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.067"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            18..791
                     /locus_tag="OO145_002421"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO145_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO145_002422"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO145_002422"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5467515.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO145_002423"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO145_002423"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5467516.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO145_002424"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO145_002424"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5467517.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO145_002425"
     CDS             complement(4989..7223)
                     /locus_tag="OO145_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5467518.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO145_002426"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO145_002426"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5467519.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO145_002427"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO145_002427"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5467520.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO145_002428"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO145_002428"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5467521.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO145_002429"
     CDS             complement(12978..14318)
                     /locus_tag="OO145_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5467522.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO145_002430"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO145_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5467523.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO145_002431"
     CDS             complement(16106..16555)
                     /locus_tag="OO145_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5467524.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO145_002432"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO145_002432"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5467525.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO145_002433"
     CDS             complement(17355..17684)
                     /locus_tag="OO145_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5467526.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO145_002434"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO145_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5467527.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO145_002435"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO145_002435"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5467528.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO145_002436"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO145_002436"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5467529.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5671 c         5067 g         5286 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcctccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTMX010000068        21168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTMX010000068 DAJTMX010000000
VERSION     DAJTMX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.068"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            376..815
                     /locus_tag="OO145_002437"
                     /pseudo
     CDS             376..815
                     /locus_tag="OO145_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="OO145_002438"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="OO145_002438"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5467530.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="OO145_002439"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="OO145_002439"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5467531.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="OO145_002440"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="OO145_002440"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5467532.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="OO145_002441"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="OO145_002441"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5467533.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="OO145_002442"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="OO145_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5467534.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="OO145_002443"
     CDS             complement(8300..11167)
                     /locus_tag="OO145_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5467535.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="OO145_002444"
     CDS             complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="OO145_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5467536.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13219..13803)
                     /gene="pth"
                     /locus_tag="OO145_002445"
     CDS             complement(13219..13803)
                     /gene="pth"
                     /locus_tag="OO145_002445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5467537.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14081..14359
                     /gene="ychH"
                     /locus_tag="OO145_002446"
     CDS             14081..14359
                     /gene="ychH"
                     /locus_tag="OO145_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5467538.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="OO145_002447"
     CDS             complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="OO145_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5467539.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16218..17165)
                     /gene="prs"
                     /locus_tag="OO145_002448"
     CDS             complement(16218..17165)
                     /gene="prs"
                     /locus_tag="OO145_002448"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5467540.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="OO145_002449"
     CDS             complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="OO145_002449"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5467541.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="OO145_002450"
     CDS             complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="OO145_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5467542.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19004..20260
                     /gene="hemA"
                     /locus_tag="OO145_002451"
     CDS             19004..20260
                     /gene="hemA"
                     /locus_tag="OO145_002451"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5467543.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20302..>21168
                     /gene="prfA"
                     /locus_tag="OO145_002452"
     CDS             20302..>21168
                     /gene="prfA"
                     /locus_tag="OO145_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5467544.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQA"
BASE COUNT         5244 a         5297 c         5322 g         5305 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttactatg aatatggcaa aaatattaca acaaacagcg gttaaaaaat
    11461 ttggtgaaga ttttttgtaa taatcctaat tagttatggg attttcgctt gatgtatctg
    11521 cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg
    11581 atattcattt ttgttcaatg gattttctgg cctatcgtta ttattgtgct actggttatc
    11641 attttcatct tgccaggatc gcacgtaatc acgttattcg gggactttat ctgcgggata
    11701 ttacccttca ggtaagtcct tttctcgcca gcatattgga gcgagatcaa tattgatcag
    11761 ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg ttatatcact gctaatgaga
    11821 tatcagtcat ctgaacactt atgtctttaa ttattctcgt ggttcactgt aggcaataag
    11881 cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa aatccacgca
    11941 attgcgtgga ttttatatac ttttgcgctc ttcatgagat ttagcgaaac ctcatgagac
    12001 aataaattaa ttagacgttg aaaaggaagt tcatcacatc gccatctttc acgatgtaat
    12061 ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct ttgtaagtga
    12121 tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa tcagtatgga
    12181 ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca cgcacttctt
    12241 tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca cggatcacac
    12301 ggttcaggcc cggttcttcc agcccaagct cctgcataaa ctcgtcacgt tcttcgtcgt
    12361 ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca gaaccttctt
    12421 tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaacca tcttcgttga
    12481 cgttggctat gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt
    12541 tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc gttttccagc tggggcaggc
    12601 atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct
    12661 gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga
    12721 taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt
    12781 caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt
    12841 tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca
    12901 tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc aggcgagggt
    12961 caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt
    13021 caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga
    13081 cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt
    13141 attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa
    13201 aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt
    13261 caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg cttcatcaat
    13321 taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc
    13381 gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact
    13441 gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaacttaa atttggcgac
    13501 gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa
    13561 aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa
    13621 caggcggaca tcttcgcttc caagggtgac tcgcgaagta taaccaaaga atttagcctc
    13681 ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt
    13741 atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt
    13801 cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcggccccg
    13861 cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcctgag tggactatta
    13921 gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg
    13981 tgattacgcc cgcacataac ccacagggtg ttgtctatac tttacacata aggaagaggg
    14041 gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc
    14101 gatactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta
    14161 ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt
    14221 gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga
    14281 acaggtgtgc gaccgttact ggtgggttcg tcactatgac aaacgttgcc gccgtagcga
    14341 taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg
    14401 gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc
    14461 gtcccgggat cggttgaatg cctgcgcgag ccatagttcg cagtggctgg aattccacgt
    14521 tgcacacgcg cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa
    14581 gaccaccagc atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt
    14641 caagacgtga ctccaggtcc gtgaataagc cttcagcagc agcaaaaaac agcgggccaa
    14701 taacgcgcag aaccaggaca tcgtctggaa catctacgac taccggtgcc aggcgagtca
    14761 tacgtgcgat acgacgcata aacagcagcg atgccagcac gatccccacg ctgatggcaa
    14821 taaccatatc aaacagcacg gtcagtgaca tacacagcag catgacgatg atgtcatctt
    14881 tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc actcatgttc cacgccacca
    14941 tcaacagcag ggctgccatg gcggaaagcg gcagccagga gagcagtggt gccagtacca
    15001 gcagggcgag aataaccaga atagagtgga tcaccgccga gataggggaa gttgccccgg
    15061 cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc ggtaatacca ccaaagaatg
    15121 gagcgatgat attccccagc ccctgaccga ccagttcact attcgccttg tgcttagtcc
    15181 cggtcatacc atccagtacc acagcgcaga gcagagattc gatcgccccg agcattgcca
    15241 ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca ggttagcgtg aattctgaat
    15301 taggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag
    15361 aaccatcggc caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca
    15421 ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac
    15481 gaatgcccag acgcggccag aaaaccagaa tgccaagcgt cacaatgcca atggcagcat
    15541 cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt
    15601 ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga
    15661 tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcagacgac
    15721 caaagcgtgc cagccccatc agaatcaaaa agattcccga caacaaggtc gcaaccagca
    15781 gtcctgccag tccaaactgt tgcgaaacgg gatagagaat taccacaaat gccgcagtcg
    15841 gaccggaaac gctaaagcgt gacccacccg ttagagcaat gacaatcccc gcaacagctg
    15901 cggtatataa accgtactgg ggggccacac cactaccaat agccaacgcc atcgccagcg
    15961 ggatagcaat aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag
    16021 tatatttttc tttccagcaa acgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa
    16081 atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg
    16141 tgaaggaggc ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt
    16201 ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata
    16261 cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat
    16321 ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta
    16381 cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt
    16441 ttagcaccac gttctttcag cgcttcagca gctttacaca gcgtaccgcc agtgtcgatc
    16501 atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg
    16561 gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc
    16621 ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta
    16681 tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc
    16741 ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca
    16801 acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa
    16861 cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct
    16921 gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta
    16981 ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc gttaatttgt
    17041 acgctgactt cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg
    17101 cggttggcaa tacgttgtgc tagtaccggg gtggcgttac cagcaaaaag cttcatatca
    17161 ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc
    17221 gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca
    17281 aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg
    17341 gaaagattaa cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc
    17401 tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg
    17461 cgtgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt
    17521 cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt
    17581 tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca
    17641 ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca
    17701 acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc
    17761 agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc
    17821 accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc
    17881 ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt
    17941 ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg
    18001 ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa
    18061 tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg
    18121 tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc
    18181 agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca
    18241 tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct
    18301 ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac
    18361 ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt
    18421 tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact
    18481 gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca
    18541 gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt
    18601 ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt
    18661 gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa
    18721 cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg
    18781 gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag
    18841 cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt
    18901 cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc
    18961 aagcagacta accctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact
    19021 cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga
    19081 taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt
    19141 gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca
    19201 agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa
    19261 aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg
    19321 cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgt
    19381 cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt
    19441 ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc
    19501 ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt
    19561 gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt
    19621 acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt
    19681 tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat
    19741 catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt
    19801 aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga
    19861 gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat
    19921 catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc
    19981 tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg
    20041 cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc
    20101 ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa
    20161 ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga
    20221 acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt
    20281 ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat
    20341 gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa
    20401 cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc
    20461 gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga cgatcctgaa
    20521 atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa
    20581 cagcaattac aggttctatt actaccaaaa gatcctgatg acgaacgtaa cgccttcctc
    20641 gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt ttgcaggcga tctgttccgt
    20701 atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag
    20761 ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt
    20821 cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg ttcctgctac ggaatcgcag
    20881 ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa
    20941 ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt
    21001 ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt
    21061 gttgttgaat gtcaggacga acgttcacaa cataaaaaca aagcgaaggc gctttcagtg
    21121 ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc aacaggcc
//
LOCUS       DAJTMX010000069        21161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTMX010000069 DAJTMX010000000
VERSION     DAJTMX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.069"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            95..2248
                     /locus_tag="OO145_002453"
     CDS             95..2248
                     /locus_tag="OO145_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5467545.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO145_002454"
     CDS             2301..4031
                     /locus_tag="OO145_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467546.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO145_002455"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO145_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5467547.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO145_002456"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO145_002456"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5467548.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO145_002457"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO145_002457"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5467549.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO145_002458"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO145_002458"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5467550.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO145_002459"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO145_002459"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5467551.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO145_002460"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO145_002460"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5467552.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO145_002461"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO145_002461"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5467553.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO145_002462"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO145_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5467554.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO145_002463"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO145_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5467555.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO145_002464"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO145_002464"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5467556.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO145_002465"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO145_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5467557.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO145_002466"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO145_002466"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5467558.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO145_002467"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO145_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5467559.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO145_002468"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO145_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5467560.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO145_002469"
     CDS             16289..16828
                     /locus_tag="OO145_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5467561.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO145_002470"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO145_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5467562.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO145_002471"
     CDS             complement(18187..18534)
                     /locus_tag="OO145_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5467563.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO145_002472"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO145_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5467564.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..>21161)
                     /gene="secD"
                     /locus_tag="OO145_002473"
     CDS             complement(19645..>21161)
                     /gene="secD"
                     /locus_tag="OO145_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5467565.1"
                     /translation="WLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLR
                     SDLREKGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAV
                     MSDARLSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKE
                     ILGATATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDS
                     TSSQDEYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLV
                     KQEEVINIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTL
                     GMQNIEQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLS
                     MPGIAGIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTL
                     IKVIILYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
BASE COUNT         5685 a         5293 c         5084 g         5099 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaatcg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag c
//
LOCUS       DAJTMX010000070        20779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTMX010000070 DAJTMX010000000
VERSION     DAJTMX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.070"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..579
                     /locus_tag="OO145_002474"
     CDS             <1..579
                     /locus_tag="OO145_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5467566.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO145_002475"
     CDS             597..1235
                     /locus_tag="OO145_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5467567.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO145_002476"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO145_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5467568.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO145_002477"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO145_002477"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5467569.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO145_002478"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO145_002478"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5467570.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO145_002479"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO145_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5467571.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO145_002480"
     CDS             complement(5552..6613)
                     /locus_tag="OO145_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5467572.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO145_002481"
     CDS             complement(6610..8007)
                     /locus_tag="OO145_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5467573.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO145_002482"
     CDS             8163..9161
                     /locus_tag="OO145_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5467574.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO145_002483"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO145_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO145_002484"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO145_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO145_002485"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO145_002485"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5467575.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO145_002486"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO145_002486"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5467576.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO145_002487"
     CDS             complement(14232..14855)
                     /locus_tag="OO145_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5467577.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO145_002488"
     CDS             complement(14852..15274)
                     /locus_tag="OO145_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5467578.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO145_002489"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO145_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO145_002490"
     CDS             complement(16089..17141)
                     /locus_tag="OO145_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5467579.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO145_002491"
     CDS             complement(17172..18014)
                     /locus_tag="OO145_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5467580.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO145_002492"
     CDS             complement(18001..18882)
                     /locus_tag="OO145_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5467581.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO145_002493"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO145_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OO145_002494"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OO145_002494"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5467582.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5668 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAJTMX010000071        20709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTMX010000071 DAJTMX010000000
VERSION     DAJTMX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.071"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO145_002495"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO145_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5467583.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO145_002496"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO145_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5467584.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO145_002497"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO145_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5467585.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO145_002498"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO145_002498"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5467586.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OO145_002499"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OO145_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5467587.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OO145_002500"
     CDS             complement(8218..9111)
                     /locus_tag="OO145_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5467588.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO145_002501"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO145_002501"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5467589.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO145_002502"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO145_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5467590.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OO145_002503"
     CDS             complement(10242..11474)
                     /locus_tag="OO145_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5467591.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO145_002504"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO145_002504"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5467592.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO145_002505"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO145_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5467593.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="OO145_002506"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="OO145_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO145_002507"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO145_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5467594.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO145_002508"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO145_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5467595.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO145_002509"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO145_002509"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5467596.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO145_002510"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO145_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5467597.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5030 g         4971 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaat
//
LOCUS       DAJTMX010000072        20644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTMX010000072 DAJTMX010000000
VERSION     DAJTMX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.072"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            96..395
                     /locus_tag="OO145_002511"
     CDS             96..395
                     /locus_tag="OO145_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5467598.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO145_002512"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO145_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5467599.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO145_002513"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO145_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5467600.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="OO145_002514"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="OO145_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5467601.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="OO145_002515"
     CDS             complement(2534..2878)
                     /locus_tag="OO145_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5467602.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="OO145_002516"
     CDS             3030..3260
                     /locus_tag="OO145_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467603.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="OO145_002517"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="OO145_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5467604.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="OO145_002518"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="OO145_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5467605.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="OO145_002519"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="OO145_002519"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5467606.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="OO145_002520"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="OO145_002520"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5467607.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO145_002521"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO145_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5467608.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO145_002522"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO145_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5467609.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO145_002523"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO145_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5467610.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO145_002524"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO145_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO145_002525"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO145_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5467611.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO145_002526"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO145_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5467612.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO145_002527"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO145_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5467613.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO145_002528"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO145_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO145_002529"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO145_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5467614.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO145_002530"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO145_002530"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5467615.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO145_002531"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO145_002531"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5467616.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO145_002532"
     CDS             complement(19399..19827)
                     /locus_tag="OO145_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5467617.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO145_002533"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO145_002533"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5467618.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20538..20614)
                     /locus_tag="OO145_002534"
     tRNA            complement(20538..20614)
                     /locus_tag="OO145_002534"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20578..20580),aa:Arg,seq:acg)
BASE COUNT         4983 a         5279 c         5153 g         5229 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg atacatcggg aaacttactt tactgcagat
    20641 tttt
//
LOCUS       DAJTMX010000073        20564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTMX010000073 DAJTMX010000000
VERSION     DAJTMX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.073"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            694..1716
                     /locus_tag="OO145_002535"
     CDS             694..1716
                     /locus_tag="OO145_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5467619.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1716..2696
                     /locus_tag="OO145_002536"
     CDS             1716..2696
                     /locus_tag="OO145_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5467620.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2693..3451
                     /locus_tag="OO145_002537"
     CDS             2693..3451
                     /locus_tag="OO145_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5467621.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3461..4270
                     /locus_tag="OO145_002538"
     CDS             3461..4270
                     /locus_tag="OO145_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5467622.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4270..5124
                     /locus_tag="OO145_002539"
     CDS             4270..5124
                     /locus_tag="OO145_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5467623.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5150..5707
                     /locus_tag="OO145_002540"
     CDS             5150..5707
                     /locus_tag="OO145_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5467624.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            5644..7104
                     /locus_tag="OO145_002541"
     CDS             5644..7104
                     /locus_tag="OO145_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5467625.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(7154..7408)
                     /locus_tag="OO145_002542"
     CDS             complement(7154..7408)
                     /locus_tag="OO145_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5467626.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7609..8343
                     /locus_tag="OO145_002543"
     CDS             7609..8343
                     /locus_tag="OO145_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5467627.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8345..8956)
                     /gene="emtA"
                     /locus_tag="OO145_002544"
     CDS             complement(8345..8956)
                     /gene="emtA"
                     /locus_tag="OO145_002544"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5467628.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9056..9970
                     /gene="ldcA"
                     /locus_tag="OO145_002545"
     CDS             9056..9970
                     /gene="ldcA"
                     /locus_tag="OO145_002545"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5467629.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10065..11801
                     /locus_tag="OO145_002546"
     CDS             10065..11801
                     /locus_tag="OO145_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5467630.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12412..13826
                     /locus_tag="OO145_002547"
                     /pseudo
     CDS             12412..13826
                     /locus_tag="OO145_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13994..15064)
                     /gene="dadX"
                     /locus_tag="OO145_002548"
     CDS             complement(13994..15064)
                     /gene="dadX"
                     /locus_tag="OO145_002548"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5467631.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15074..16372)
                     /gene="dadA"
                     /locus_tag="OO145_002549"
     CDS             complement(15074..16372)
                     /gene="dadA"
                     /locus_tag="OO145_002549"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5467632.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16702..18234
                     /locus_tag="OO145_002550"
                     /pseudo
     CDS             16702..18234
                     /locus_tag="OO145_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            complement(18286..19005)
                     /gene="fadR"
                     /locus_tag="OO145_002551"
     CDS             complement(18286..19005)
                     /gene="fadR"
                     /locus_tag="OO145_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5467633.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            19226..>20564
                     /gene="nhaB"
                     /locus_tag="OO145_002552"
     CDS             19226..>20564
                     /gene="nhaB"
                     /locus_tag="OO145_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5467634.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSV"
BASE COUNT         4989 a         5371 c         5128 g         5076 t
ORIGIN      
        1 tggagaatgt tatcattgct acaccctgtg cttaataact gcaactctcc gccgttcagc
       61 gatgctttga taattctgtt cccatagata tcgttatcaa aataaagcgt aaatatcata
      121 tttccgtaac tgttatcatc tctttttttg cacgtccgaa taaacgcatt cagtaaatta
      181 ctctctctaa gaaaataaat atcttcattg ttgttaatga cattaataac gtcgttctta
      241 aaatcctcaa agtcttgttg cggtaacttc tggttacgct gcttactatc agcaagcggt
      301 gaatccggac gacaacaaca taggttttgt ataaccccca gcattctttt cacctccgtt
      361 aacagtatat tcacgacact gaaacgagag aatgctgata attttctcgg cgagatatca
      421 aaaatcgctc attataaaac aagtaacttc aacgtcgcgt aaccgtggac gataaaaaaa
      481 caataatgtc attcatattt aaataattta gccttaaatg atttaaataa tattagccac
      541 ctgataaata catttttaat gatagagagg gtcaacaggc ttgcggaagt cgtcacatct
      601 tcatgttttc aatagctaaa ctttgatatt gaacatgatt tttatcgata tatatgaaag
      661 tatatatcga ttttactttt caggatgttc accatgccca taaccagacg gacgtttgcg
      721 caggcacttg catctaccct gctcctgcag tctctaccgt ccttttcaca aacagttaat
      781 cgttttgctt cgcaatctct tcccgaagcc caaaacatta cacgcatcgt cagcgcaggc
      841 gcgcccgccg atttgctctt gctggctgtc gcgccagaaa aaatggtcgg tttttcctct
      901 tttgattttg cccgtcaggc attaattcca ttgccagagc acattcgcca gttacccagg
      961 ctgggacgac tcgccgggcg cgccagcaca ctatcgctgg aagggcttat ggcgttacat
     1021 cccgatttgg tcgttgattg cggcaatacg gatgaaacct tgatctccca ggcacggcag
     1081 gttagcgaac agacacaaat cccctggtta ttgcttaacg gaaaactggc gcaatcagca
     1141 gaacagttaa caacgcttgg caaaacgtta ggcgaagagc accgcgccgc agaacaagcc
     1201 aatctcgcca gccacttcgt tggtgaagct caggcattcg ccacctcacc cgccgctaac
     1261 ctccgctttt atgctgcgcg cggtccccga gggctggaaa cgggcttaca gggttcgttg
     1321 cataccgagg cggcggaatt attaggtttg cacaacgtcg cgcaaatagc cgatcgccac
     1381 ggcctgacac aggtttccat ggaaaatctc ctgcgctggc agccggatat tattctggtt
     1441 caggaggccg ttactgcaga ttttattcgt cgtgatcctc tctggcaggg cgtgaaagcc
     1501 gttgcggaac aacgcatctt atttttaagt ggcttgccct ttggctggct cgatgccccg
     1561 ccgggaatca accgtcttct gggattacgc agacttcacg cctggctgga tcccgccatc
     1621 aatcgccagt ttaaaagtga catgcagcat tacgcccaac tgttctggca ttgttcactc
     1681 agtgatgccg actatcaaaa attggtggcg agctaatgag aatcgtaaac ggatgtattt
     1741 tgctgctggc agctatcagt atcacttttg ccgccgtaag tggggcttat catctcgata
     1801 tgcagcaact gctggcgcta attcttcgtc aagaaaatga gtctgtgcag gaacaaattg
     1861 tcttctggca aatccgcgtg ccgcgtattc ttgctgcgct gttactgggc gcagcgctgg
     1921 caggagccgg aaccacctat cagggaatgt tgcgtaatcc cttagtttca ccggatattt
     1981 taggcgtctc cgccggtgcc ggacttggtg cctgcgcggc aatcctgtgg ggattaccca
     2041 ttgtttacat ccagctgtac gcattttgcg gcgggctgat ggtggtcgcg ggcgtctggt
     2101 tgattacgcg tcgggtgacc cgccacgatc cgatcttaac cctggtgctg gtaggcattg
     2161 cgttaggaac gttgtgcgga gcggggatat cgctaattaa aacgctggcc gatccttaca
     2221 cccagttacc gtcaattacc ttctggttgc tggggggact gtccaccgtg accttacgcg
     2281 atctctgcta tgctgcaccg atcattctca taggctcctt gcccctgttt tttctccgct
     2341 ggcgcatgaa tttactcacg ctttccgacg acgaagcccg ttcgctggga ctgaacgtga
     2401 cgcgtctgcg ctttggattg attgtctgcg ccacactaat caccgccagc acggtagcaa
     2461 tagcgggcat aattggttgg atcggtctgg tcgttccgca cattgcccgc ttgttgaccg
     2521 gtcataacca tcagcagctg ttgcctatgg caatgtgtac tggcgctatt ctgctcctgc
     2581 ttaccgatac gctggcgcgc agcattggaa caacagaaat cccacttggc attctgaccg
     2641 cttttgttgg cgcaccgttt ttcctgcttt tgttgttacg cggaggtcgc cagtgacttt
     2701 gcttgccgtt cgtcatgcgt ctctgggtta ctcccgccat ccagtgctac gcgacgtgtc
     2761 cttcaccctg tcgcaaggaa caatttgttg tctgcttggc gcgaatggtt gcggcaaaac
     2821 cacactcatg cgctccattc ttggcgttat accgttatta aaaggcgaga tcctactgga
     2881 tagcctctct gtgcagacac tcagccatcg ccagcgcgca caggctattg cctgggtgcc
     2941 gcaggcgcac gacggtattt ttgctttcag tgtgctggat atggtgttga tgggcctggc
     3001 ccccaccata ggcgcttttt ccgttcccgg taagcaagaa cgcctgaaag ccacggagca
     3061 actggaaaag ctgggcattt tgcatctggc gacacgacgc tggaatacgc ttagcggcgg
     3121 tgaacgtcag ctggctttaa tcgcccgtgc actcgtccaa caaccgcgct tgttgttact
     3181 ggatgagccc gcctccagcc tcgatttcgg tcatcagatc caacttctgg acacgctggc
     3241 gcagctcaaa aataacggca tgacaatgtt aatgtcgact caccatacgc tccatgccaa
     3301 cgccattgcc gacagcatta tccaagtaga gccagatgga cgagtaacac aaggcttacc
     3361 gaccgaacag ttaacaacga acaaactggc ggccctgtat cgggtttctg ccgaccaaat
     3421 tcatcatcac ctctctgcaa ttagccgcta aggaacaatc atgctcattg atgatattga
     3481 ttttgcagac ctttatctgc agcaactcaa actggctcac cgcacagaaa aaacgcccga
     3541 tcactgggat caacgggcgg agaaaatggc ggaaaattgc gcctcgccaa cggacagcta
     3601 cctgcaacaa ctgatcgcta aaattgacct gcaaggcgct cagaccttat tcgatatggg
     3661 ctgcggacca ggcaccgtat cgctggcgct ggctgacaaa ctcactacgg tttatggcgt
     3721 tgattacagt caggggatgc tgaacgttgc cgcccgccgc gccgccgcac tgaaggctga
     3781 caatgtccac tggatccagc gcgcctggga agaggactgg agtgacctgc cacgctgcga
     3841 tattgccgtg gcttcccgct ccactctggt ggctgatatg cgccatgcaa tgagcaaact
     3901 gaataatcag gcacggcttc gcgtctatac aactcatctg gtcagcacct cgttcgtctc
     3961 tccagccatt cagcgggcgg ctggtcggga agtgatcgaa ttacccaact atatctttgc
     4021 gctcaacgtc ctgtatcaga tgggaattta tgcccacgtt gattttatcc gtggacaaaa
     4081 ttgccaacag gataacagca cctgggaacg ttttgagcag aacgtcagct ggagtcttgg
     4141 cgcgcttaac gacgatgagc gtgagcggtt atatcactgg tatcaacaac aagatgctcg
     4201 tgccctcgcc cctgccagcc gcgactgggc gctcatctgg tgggatagcg taccgcagga
     4261 ggcactgtga tgattttcct ttctcaggca caaatcgatg cgttactgct ggaagatatc
     4321 cagggaggtg acctgaccac gcgagcatta aatattggac accagcctgg ctatatagcg
     4381 ttttttcacc gtcagggcgg ctgcgtcagc ggcgtttctg tcgcgtgtaa gatgttaact
     4441 acgctgggat taactattga tgacgcggtc agcgacggtt cacaagcgaa cgcgggtcaa
     4501 cggctaatcc gtgcgcaggg taatgccgca gcacttcatc agggatggaa ggcagtccag
     4561 aatgtgctgg agtggagttg cggtgtttct gattatctcg ctcaaatgct ggcattgctt
     4621 cgtgaacgtt atcctgacgg caatatcgcc tgcacccgaa aagcaattcc gggcacccgc
     4681 ttactggctt cgcaggcaat tctggctgcc ggaggactaa ttcatcgtgc cggatgtgcg
     4741 gaaaccatat tattgtttgc caaccaccgc cattttcttc atgacaatca gggctggtca
     4801 ggcgcaatcg ctcagttacg tcgccacgca cccgagaaga aaattgttgt tgaagccgac
     4861 acgccgaaag aggctatcgc cgcgttacgc gcacaaccag acgtacttca gctcgacaaa
     4921 tttagtccgc aacaggcaac tgaaattgcg caaatagcgc cgtcgctggc tcctcactgc
     4981 acgctggctc tgaccggcgg aattaatctg acgacactca aaaattacct cgactgcggc
     5041 attcgccttt ttattacctc cgcgccttat tacgcggcac cagctgacat taaagtcagt
     5101 ctgcaaccca cagcctctat ttaattcttt gttaaacgga atttattcta tgagactcaa
     5161 aaaacgttat ctctgcacag cattatcact tgcctttacc cagcaggccg tagcggctca
     5221 ggagagcgac acgctgaccg tatggtccag tccggtatca tcgacgacga ccaccgttct
     5281 cgatcaaccc accatgaagg ccttggataa acagaatgtc gctcaggcat taagtgtcgt
     5341 ccccggcgtg gtgctgcaaa agtcaggcag ccgcaacgaa gaacaggtaa aagttcgtgg
     5401 ctttgatagt cggcaggtgc cggtctattt cgacggtgtg cccatttatg tcccctatga
     5461 cggcaacctc gatctggcgc ggattctgac caacaatctg ggggcagtcg aagtttccaa
     5521 agggtattcg tcgctgcttc atgggcctaa tcagatgggc ggagccatta atatcaccac
     5581 cctgaagaag caagtctggg atatcgccag ggatggagcc gtagtcagga caatgcctac
     5641 gatatgcatg cttcatttgc cgccagcagc gatcttgggt atttgcaagt cagcggtagc
     5701 cagctaaagc aggattttct cggcctgccg catggtgtaa ataatgatat tgcaggcaaa
     5761 cacggcaaga tgattaattc atcggctgat gataaacgcg gcattgtgaa gctcggtttt
     5821 acaccgcgcg aaaacgatga atacacgctg acttacatta agcaggatgg tgaaaaagat
     5881 aacccgccat acagcggaaa ttgtggtcaa aaatcacgct actggcagtg gccagagtat
     5941 gacaaagaaa gtttttatta tcagggagcg acccaactaa acgaccgttt taccctgaaa
     6001 agtcggctgt atcgcgacac ctttgaaaat acgctgatga tgtacaactc gctggctgat
     6061 ttgaaaaata aaaaaggcag ctacagccat tattccgatt acagcgacgg tgccggatta
     6121 caactggcag ccgatgtgcg tgaaaacgat ctgctgtcgt ttgccgttaa ctggaaagat
     6181 gacgtacacc gggaaaaagg tgcgccgcac gccgcttacg atcgctatga agatcgtacc
     6241 tggtcgctcg ccagtgaata tcaatgggct gctgctgata atgtcgatgt cgtggcggga
     6301 atcagctatg actggcgcga tagcgtagaa gcgaaaaaac acgagaaaga tggcagcatc
     6361 acccattatg acgacaacaa tcagtcagct tttaactggc aggtgatggg gaaataccac
     6421 tttgccaatg aagacactct ggcgctttcg tactatgacc gcacacgctt tccgacgctg
     6481 aaagaacgct ataccacatc caaacctgcg tataaccaga tagcgattgt taacccgcag
     6541 ctcaagccgg aacgcgctcg cggggtggat ttaacctgga atggtgcctt cacgcgcgac
     6601 tggggatttg aggtcagcgt ttactataac cgggtgagtg atgccatcct ctcgcacaat
     6661 atcaatgccg ataccattca aaatcagaac agcggcacgg tggattacag cggtctggat
     6721 gccggtatta aggggaaaat cagcaatata ctggatgtag gattgagcta cgctctgatc
     6781 cacgctgacg ccaaacgcaa agacatcggc aagataaccg atcttccaac gcagacaatg
     6841 accgcatgga tgacactcaa accatgggag ccgttaagcg taacgctgtc ggaagaggcg
     6901 cgttcctcca gctacagcaa cagtgacggt tcacaaaaag ccgccggttt tgcggtgacc
     6961 cacattcgag ccgattacac cttaggtcat ggcttcagcg tcaatgcgtc ggtcaataac
     7021 ctgtttgata cccaatatgc ctacagtgaa gggtttattg aagaaggtcg caatttctgg
     7081 gcaggtgtcg agtatacgtt ctgatgtgaa agcggatagc ggcatctgta tcgctatccg
     7141 ccttatgaaa atcttattct cttcgcagga ggcggaatac gcccagcacc aggatagcgc
     7201 ccaccaccgc caccaggaag ctgtgcaaat taaaaccgct gatggagccg tcaatgccaa
     7261 acatggtcgc cagccagccg ccgaccaccg caccgactat cccgagaata caggtcagga
     7321 aaaatccacc accatcacgc cccggcatga ttagcttgcc gataatgccg gctatcaggc
     7381 caaaaataat ccaggcaata attcccatgt tcaggccctc gtttcgatga atcggtcgca
     7441 agaaatgctt acgtcaacgc tctaagtata ggcaagggcg ggatcgatgc tgggcgtttg
     7501 tcggctgatt taagaacaac atgtaaaaaa tattcacgca tttgtattaa gttttgttaa
     7561 ctatgaccga taaaccaaag acagtttgtc agtcaggagt ttttccgcgt gagtcattac
     7621 catgagcagt tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac
     7681 aaagccgcaa ttcctttgcg tctcagttgg aatggcgggc agctgatcag caaaatactg
     7741 gcaataaccc cggataaact ggtgctggat ttcggcagtc aagccgaaga caacatcgcc
     7801 gtgctaaagg cacagcacat taccattacc gccgaaactc agggtgcgaa agtcgagttt
     7861 actgttgaac aactacagca gagtgaatac ttgcagcttc cggcatttat taccgtaccg
     7921 cctcccacct tatggtttgt acaacgacgc cgatatttcc gcatctccgc cccactccat
     7981 ccgccttatt tttgccagac caaactggcg gataacagta cgttacgttt ccgcttgtat
     8041 gatttgtcgt taggcggcat gggcgcatta ctggaaacag caaagcctgc cgaattacaa
     8101 gaaggtatgc gcttcgctca aattgaagtc aacatggggc aatggggtgt ttttcacttt
     8161 gacgcccagt taatctccac cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc
     8221 accactcccc gtctgagctt ccgttttctt aacgtcagcc cgacggtgga gcggcaatta
     8281 cagcggatta ttttctctct cgagcgagaa gcccgggaaa aagcggacaa agtgcgcgac
     8341 tgatttacat cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg
     8401 caggatgatt tcgcgctacg tgatcgagga actcgtcagc atccaaatcg ttgattttgc
     8461 tgatcgcctt tttccgatct gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt
     8521 atgacaccac cagcgcatat tgcagtacct tcggatcttc aatgcctgcc agcgggccgg
     8581 tttccagaat attcaggtaa gccgccccca ttgaaatatt acgctctgga tttttcagct
     8641 cgctggtcgt cggctcacca ctccagccca tacggcgata aacatcacgt ccggaggttg
     8701 aagcttttaa ctgcatcaaa ccaatggcat tcgatttact caccgcgttc ggattaccac
     8761 ccgattcgat agcgataatc gccgtgatca attgtggatc gacgccccag gctgcaccgg
     8821 ctttctggct tattggcatc cactgcatcg cacgttgcac cggaactttc gcgttccacg
     8881 gcgggttcgt atagtcatgc tttgatgaac aacccgctaa taacacaatc aaaaaggcaa
     8941 accatctcaa tttcacttct tctatcctta tagcctggaa tcgccgttga ggtgacaacg
     9001 gcacacctaa gcggcatgat atacatttcg tttctgttgt cagcaaggaa ttgccatgtc
     9061 tctgtttcac ttaattgccc catcgggtta cagcattaaa cagcacgccg cgctgcgtgg
     9121 tatccagcgc cttaccgacg cggggcatca agtcaataac gtcgaggtta ttgcccgtcg
     9181 ttgtgagcgt tttgcgggta cggaaacgga gcgtctggaa gatctcaatt cccttgccag
     9241 actgactacc cccaacacca tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt
     9301 actggcagat attgactggc aggcactggt ggctcgccaa caacatgacc cgttactcat
     9361 ttgcggacat agcgatttta ccgccattca gtgcggtctt ctggcgcaag gcaatgtcat
     9421 cacctttagc ggccccatgc tggtagcaaa ctttggtgcg gatgaactga acgcctttac
     9481 cgagcaccat ttctggctgg cgttacgcaa tgaaaccttc accattgaat ggcaagggga
     9541 aggcccgaca tgtcagaccg aaggcacgtt gtggggaggc aatcttgcga tgctgatttc
     9601 actgattggt acaccgtgga tgccaaaaat tgagaacggt attctggtgc tggaagatat
     9661 taacgtgcac cctttccgcg tcgaacgtat gctgttgcag ctctatcatg cgggaatttt
     9721 gccacgtcag aaggcgatta ttctcggtag ctttagcggc agcacgccca atgattatga
     9781 cgcaggttac aacctggagt cagtgtacgc gtttttgcgt tcccgcctgt cgattccact
     9841 cattaccggc ctcgattttg gtcatgaaca acgcacggtt acgctgccgt tgggcgcaca
     9901 tgccattctg aataacaccc gggaaggcgc tcagttgacg attagcggtc atcctgttct
     9961 taaaatgtaa aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc cgctaatatg
    10021 gtagggttta tataagagac agcgtaatca ggagtaaccg accattggat gccacaacaa
    10081 taattagcct tttcatctta ggatccatcc ttgtcaccag cagtatatta cttagttcat
    10141 tttcttcccg tcttggcatt cctattctgg ttatttttct ggcgatcggc atgctggcgg
    10201 gagtcgatgg cgtcggcggt atcccgtttg ataattaccc cttcgcctac atggtaagta
    10261 acctggcgct ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc agttcctttc
    10321 gtgtggcgtt aggaccggcg ctgtcgctgg cgacgctggg cgtacttatc acctctggtt
    10381 taaccggcat gatggcggcg tggctgttta atcttgattt gattgaaggc ttattaatcg
    10441 gcgctatcgt cggctccacc gatgctgcag cggtcttttc tctgctgggt ggtaaggggc
    10501 ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg
    10561 tctttctgac gattaccctg attgcaatga tccagcagca tgaaagcagt gtcagttgga
    10621 tgttcgtggt cgatattctg cagcaatttg gcctcgggat tgtcatcggt cttggcggtg
    10681 gttatttact gctgcaaatg attaatcgca tcgccctgcc tgccggatta tatccattgc
    10741 tggcattaag cggcggtatt ttaatttttg ctttaaccac ggcgctggaa ggcagcggta
    10801 ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc aaccgctacg
    10861 gcatcctgca aaatttcgac ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc
    10921 tggggctatt ggttaaccca agcgatctgc tgcctattgc cattccggcg ctcattttgt
    10981 cggcatggat gatattcttc gcccgtcccc tttcggtatt tgctggattg ctccctttcc
    11041 gcggtttcaa tctgcgtgag cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc
    11101 caatcatcct agccgtgttc ccgatgatgg cggggctgga gaatgcgcga ctgttcttta
    11161 atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca gggaacatca ctctcgtggg
    11221 cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc
    11281 tggatattca tccggaaaat ccgtgggagc agtttgttta tcaattgagt gctgataaat
    11341 ggtgtgtagg tgcggcactg cgcgatttgc atatgccaaa agagacgcgt attgcagccc
    11401 tgtttcgtga taaccagttg cttcatccta ccggcagcac ccgactgcgc gaaggcgatg
    11461 tattgtgtgt aattggtcgg gaacgcgatc tcccggcgct cggtaaactg ttcagccagt
    11521 cgccaccggt cgcgctggat caacgcttct ttggtgactt cattctcgaa gccagcgcca
    11581 aatatgctga tgttgcgctg atatatggcc tggaagacgg gcgagaatat cgtgataaac
    11641 agcaaacgct gggtgaaatt gtccagcagt tgctaggcgc ggcaccggtt gtcggtgacc
    11701 aggtagagtt tgccgggatg atctggacgg tggccgagaa agaagataat gaagtgttga
    11761 agattggcgt tcgggtggct gaagaggaag ctgaatctta aatttaatgc agcatctatc
    11821 gccggatgcg atgctttact gaacgcagcc tgagcctgta ataaacttgt catttcctga
    11881 ttttgatatg ctcatcccaa catcagaaga aggttaaatt atggatgaca aaccattgca
    11941 ggctctggct aatgaactca aaacaccggc tcaagaaagt cagcgtaagg gctttttccg
    12001 actgtaacag gtaataaaaa ccagccccgc attaccggtc aaccatcagg ggcccctgcc
    12061 tgacatcgcc tgaacatatt cccccacgca acctgccgca ctgacatagc ccacacaata
    12121 ggcgtttcat ccgcctgtag caccggatgc atgagcgatt tcttatagtt attcctttgc
    12181 ttaataacga ttatttccgg gtcgttcact ctcgtatttt ctttaatacg tcagagctac
    12241 ctaggccgta atacagcatg taccagaccc gacacggcat ttctgaccag gaaaaaagtc
    12301 aacgatgagt agtgatatca gcgcaaatga cgtcctgtgg ctcctttgcg gtgaccacat
    12361 tcacgtctcc ggcgctgcca gaggatattt atttcaagga aacgtagcat tatggctagc
    12421 tttaaagaca tctgcccatt cgcccccgcc attcccccat cttccgttaa cccaggcgta
    12481 cgacgcccgc cagggcggct cttctcaaga atcaaatttt gcagaacatg aagtatctca
    12541 gcccgcaaaa attcagctgg ggtgatgccc cctggcagat cattgacctc agtatcgcgg
    12601 gcaaagtgaa catacaggtg gacaacaata ccatcatcac gttggggact cgtctgaatc
    12661 agcaacataa cgagttcatg atggtcgcca aatggtgcga atgggctata cagcaggatg
    12721 gtttgcaaga aaacctgcaa aaaaacctgt acgaaatcct ggaagaaaac cagcaaaaca
    12781 aacagtcaga aattccccaa gaagacctga aagaaagtct ggaagaaatc aaggaaaata
    12841 tcctggaaga aaacctgcca gcaagccgga ttgaaaaccg ggcagaagca ttgcgcagaa
    12901 tgaaggaatg tctcattaca agacggagta tgctcaatct tagcaacctg ggattgactt
    12961 cactccctga gaatttgcct ccacatctga ttgaatttta ctgcagtaaa aacgtgttga
    13021 ccgcgttacc gaaggtgatg ccaaagtggc tgctggtgct tgactgtaca gataacgtct
    13081 tgatcttgtt accgaaggtg cagccctcaa aactgatggt actgaagtgc tatgaaaaca
    13141 gtattatctg gctgcctgag ctgtcgacta acctgagagt gattaactgt tctgaaaact
    13201 tcttgcaatt tttaccgccg tcgatgcccc agtacctgta taaactatcc tgtgccggca
    13261 acaatattaa ctcaatacct gatgagatgc tggagaactt gactcgcctg aaagtatttg
    13321 actgttctag taacgatttg atctcttcac cacggctgcc gcccaaacta ataatttact
    13381 actgtggaga aaacaagttt aaaacggtac aggtgccgca gccccagagc ctgaaggtgt
    13441 ttgactgtaa tggtaacccg tgggacaaag acaatttacc gacgctgctc aaagccgtcg
    13501 agggcctgaa aaaacagcag ggcctgaagg accttttgga ctttttgcac aaggaaggct
    13561 aggttgacct ggaaggactc gaggagctgg aggaccttat ggatctggag ttcctggatg
    13621 acccggaact cctggagcgc gtgaaggtac aggaggacct ggagctcctg gatcaacagt
    13681 tgggcctgtt gagtctggaa agacaacagg actcgcagcc tgttaatcaa caatctgaac
    13741 atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgac tccgagtcaa
    13801 caatgaagcg taagcgtttt atgtagaacg cctgtgcggc gagttcaggt tccggggaaa
    13861 tagtgtccat tcttttaatg gacactatca tgggcaatgg ctctgtcaca ttaacaacta
    13921 cagttgctga ccagccggat ggatgatttt ttcgcaacca gaagacgctg cctccgatcc
    13981 ggcttacaac aagttacact gtcacaaccg ggacgcgtaa cgccagtgcg cacatcaact
    14041 catagcccac cgttccggca gcggcggcga catcatcaat tttgatctcc ttgccccaca
    14101 gctcaaccgg cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc gccagcatat
    14161 ccatcgagac ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca ggagcaccgg
    14221 taggcgcatg gcgcggataa ccgtcggcgt atcctgcggc gacaatgccg attcgctgtt
    14281 catcgcgcgc agtatagcga ccgccgtagc ccacacgctc gcccgctttt agcgtctgga
    14341 caccaataat ctcactgctt agcgtcatca ccggacgtaa tccggtattg gcgatatcac
    14401 gccactgacc ggacggcgaa gcgccataca aaataatgcc aggccgaacc cagtcaaaat
    14461 gcgcttccgg gtgccacaga gtcgccgccg aattggacaa cgaacgccga cactccagcc
    14521 cctccgccgc ctgctcaata cgcgccatcg cgccggaaat tccatcagga tgttccgctt
    14581 cagcaaaatg cgacatcagg gtcatttcgc caacattcgc cattgcccgc aactgctgcc
    14641 agacggtaag cacgcgatcg gactggaagc ccaaccgatt catcccactg ttcactttaa
    14701 gataaatatc caacggtgct tttagccgcg cattttgcag tgctttgagc tgccagttgc
    14761 tgtgtacgca ggtggtcagg cggtgctggt cataaatctc cagatcctga gcatggaaaa
    14821 atccttccag catcaggatc ggccccttcc agccgcgctc acgtaacgtt attgcctctt
    14881 ccaggttaag taatgcaaag ccatcggtgg ccccgagcgc gctccagata cgctcaatac
    14941 catgcccgta agcgttcgct tttaccaccg accagacgcg cgcgtgcggc gcggcctggc
    15001 ggacaatgga cagattctgt tttaatgcct gcagatcgag gctggcctgt atcggacggg
    15061 tcatctcgtt tccttagctg tgtgcgccat gtaaatggcc cggacgtgat ggcgtaaatc
    15121 cacggctgta gcgcgctacg cttagatcct catatgggat cgctggcgtg cgaccagaga
    15181 gcagatcgct taacaactga ccggaaccgc aagccatcgt ccagccgagc gtgccgtgac
    15241 cggtattcag ccacagattt ttaaagcgtg tactcccgac aaccggcgta ccgtctggcg
    15301 tcatcgggcg caaacccgtc cagaaagtcg cctgctcgac atgaccgccg cgtggataga
    15361 gatcgcgaac caccatttcc agcgtttcac gacgcggttg caacagctcg gtgttaaaac
    15421 caacaatttc cgccattccg ccaacacgaa tgcggttatc gaaacgggta atggcgattt
    15481 tgtaggtttc atcaagaatg gtggatactg gcgcaccatc ttcctgcgca attggaatag
    15541 tcagcgagta gcctttcagc ggatacaccg gaatatcaac aatgcctttg agcatcgccg
    15601 tcgagtaaga accaaacgcc atcacatacg catcggcctt aatcacttca tcgccaaact
    15661 tcacgccgta gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta ttaaagcgga
    15721 atttaacccc cgcctgctct gccatccgcg ccagattctg ggtaaacagc tggcagtctc
    15781 cggtttcatc attgggtaac tgcaggccgc ccgtcagttt gtgcgctact tctgccagcg
    15841 cgggatccac ttccgccagg cggctggatt ccagcagctg atacggtacg ccggcatctt
    15901 ccagcacggc gatatcgcgg gtcgcatttt catactgttg ttcggtacgg aacagttgca
    15961 gcgtcccacc ctgacgccct tcatactgaa tattggtttc ggcgcgcaat gctttcaggc
    16021 aatcacggct gtattccgcc agacgcacca tccgcccttt gttttccatg tagtggctgg
    16081 tgtcgcagtt acgtaacatt tgccacatcc atttcaactg aaactgcgta ccgtcgagac
    16141 gaaccgccag cggcgcatgg cgctggaaca tccatttaat cgcttttaaa ggcacacctg
    16201 gtgctgccca cggtgcagca tatccggggg agatttgccc ggcattcgcg gcactggttt
    16261 ccagggcggc ccccggctcc cgatcaatga cggtgacctc atgtcctgcc tgatttaagt
    16321 accaggcgct ggcaacgcct accacaccac ttcccagtat gacaactcgc atagccactc
    16381 cgataacggt agatacagta aaagaataat aatctaatta catcttgata actcagttga
    16441 aaatattatt caacatacgg cttttttatg gtgagctgac tcacatctcc ctgactctgc
    16501 ggcaatatgg cgtctttagt atctcctgct gaacgccctt tttatccagc ataaaattcc
    16561 gttcagaagc ggattagtgg cactctgata ataagaaatc gcaaagaaaa aaattctccg
    16621 gcgacgcggt ttttaacagg tgttctatgc ttgaaatgag gtgctccaca cagagagcgc
    16681 cgacaacaat gaggtgcgcg tatggcgacg atcgattcta tgaataagga caccacacgt
    16741 ttgagcgatg gacccgactg gacgttcgac ctgctggatg tttatctggc agagatagac
    16801 cgggtggcga aactctaccg gctggatacc tacccgcacc agattgaagt gataacctca
    16861 gaacagatga tggatgccta ttccagcgtc ggcatgccaa ttaactatcc gcactggtca
    16921 ttcggtaaaa agtttatcga gactgaacgg ctgtataagc acggtcagca agggctggcc
    16981 tatgaaatcg tcattaactc taacccgtgt atcgcttact tgatggaaga gaacaccatt
    17041 actatgcagg cgctggtgat ggcacacgcc tgctatggac ataactcttt ctttaaaaac
    17101 aattgcttat tccgcagttg gaccgacgcc agttcgattg tcgattatct gatttttgcc
    17161 cgtaaatata ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga acggcttctg
    17221 gactcgtgcc acgcgctgat gaactacggc gtggaccggt acaaacgccc gcaaaaaatc
    17281 tcgctgcaag aagagaaagc ccggcagaaa agtcgcgaag agtatctaca aagtcaggtc
    17341 aatatgctct ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc tgaagcgcgc
    17401 cgctatccgt ccgaaccaca agaaaacctg ctctatttta tggagaaaaa tgcgccactg
    17461 ctggaatcat ggcagcgtga aatcctgcgt attgtgcgta aggtgagcca gtatttttat
    17521 ccgcaaaaac agactcaggt gatgaacgaa ggctgtgcga ccttctggca ctataccatc
    17581 cttaaccatc tgtatgatga agggaaagta acggaacgtt ttatgctgga gtttttgcac
    17641 agccacacca atgtggtctt ccagcccccc tataacagcc cgtggtacag cggcatcaac
    17701 ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca gtcgccaacg
    17761 gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga aacgctgcat
    17821 ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agtttctgtc accgaaagtg
    17881 atgcgtgatt tccgcttctt caccgtgctg gatgatgatc ggcataatta tctggagatt
    17941 tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc gtcgcaatat
    18001 aactgaagta atctggagcc gaatattcag atctggaacg tggatttgcg cggcgaccgt
    18061 tcgctgacgc tgcgttatat tccacataat cgcgcaccgc tggatcgggg gcgcaaagaa
    18121 gtcctgaagc atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca gcaaaacgaa
    18181 gacggcagca tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct gtaaaaacaa
    18241 caaaaaaccc ctcgtttgag ggggttgctc tttaaacgga agggattatc gcccctgaat
    18301 ggctaaatca cccggcagat ttttctgcat ccggtgccaa atctcgccac tctcatgccc
    18361 atagcgacgc actgtttcgt acacctgatc gtgcgcgcct tcactgcaca acgccgacag
    18421 tttgtggtag aagcccagcg ccagactgcg cgcttccgga ttggcgaaat agtgacgacc
    18481 aatgcgcgta tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg ggttgccgga
    18541 agcaaacgcc aggccgcgga atatgttgta atccagctcg gcaaaggcat cggcgtgatc
    18601 ggccacttca ttagcggtag ccagcacttc ctgcgcttta tcgggatgct gacgaaacgc
    18661 ggtgcgaata aaaatagtgg aaatattggt acgcaccgac agcaaattat caataagctg
    18721 cggcacactt tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac cggaagtttc
    18781 ccagaaatta ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc catctcgtgc
    18841 cagacgctgt aacacttcac gtaacgtagt acgcgttacg ccaattaatt ctgaaagttc
    18901 acgttctgcg ggcaaaatag tcccgggagg gaagcggtta ttccagatac tttcaataat
    18961 gtactcttcc gcgaaacccg ccgggctttg cgccttaatg accatagtga gatttccata
    19021 acacagcaaa acaaagttgg actcatcata ccagagggct aactacgctg atagcagaca
    19081 aaaagaagaa aaagggatca aggcttcatt ttccgcatct ctttcccctt gactatcaag
    19141 acttgcctca ctcctgcctg accgttaagc tttgcgttta aacataaaaa catcattgat
    19201 tatttgtaag gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc tatggcgcaa
    19261 ttttttgggc cagtcccccg actggtacaa actcgccctc atcattttct taatcgtaaa
    19321 cccgttaatt ttcctcatca gccctttcgt cgcgggctgg ttgctggtag cggaatttat
    19381 tttcactctg gcgatggccc tgaaatgcta cccgctactc cccggtggtc tgttggctat
    19441 cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa
    19501 tcttgaagtc ttgctgttat tgatgtttat ggtggcgggt atctatttta tgaaacagct
    19561 gttgctgttc atatttaccc gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct
    19621 ctctttttgc gtggcggcag cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc
    19681 ggtggtgatc agcgttgcag tcggttttta tggtatttat catcgcgtag cctcttcccg
    19741 taccgaagac accgacctgc aagacgatag tcatatcgac aagcattaca acgtggttct
    19801 ggaacagttc cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg gcaccgcatt
    19861 aggcggcgta atgaccatgg tgggcgaacc acagaacctg atcatcgcta aagcggctgg
    19921 ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg accgttccgg ttctgatttg
    19981 tggcctgtta acctgcctgc tggtagagaa gctgcgttgg tttggctacg gtgaaacgct
    20041 gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc accagcgtac
    20101 ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc tggtgactgc
    20161 gctggcgttg catctggcgg aagttggctt gattggtttg tcagtcatta ttctggcaac
    20221 atcattgacc ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg aatctctgcc
    20281 attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg accaacaact
    20341 gttttcgccg attattcagt ttgtgttgca ggcatctgaa catgctcagc tgtcgctgtt
    20401 ctatattttc aacggtctgc tgtcatccat ttcggataac gtcttcgtgg ggacgattta
    20461 tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc acgttgaagc aatacgagct
    20521 gctggcggtc gccattaata ccggtaccaa tctgccctct gtcg
//
LOCUS       DAJTMX010000074        20521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTMX010000074 DAJTMX010000000
VERSION     DAJTMX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.074"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO145_002553"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO145_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5467635.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO145_002554"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO145_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5467636.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO145_002555"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO145_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5467637.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO145_002556"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO145_002556"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5467638.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO145_002557"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO145_002557"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5467639.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO145_002558"
     CDS             4449..4814
                     /locus_tag="OO145_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5467640.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO145_002559"
     CDS             4964..5482
                     /locus_tag="OO145_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5467641.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO145_002560"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO145_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO145_002561"
     CDS             6713..7195
                     /locus_tag="OO145_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5467642.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO145_002562"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO145_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5467643.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO145_002563"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO145_002563"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5467644.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO145_002564"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO145_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5467645.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO145_002565"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO145_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5467646.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO145_002566"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO145_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5467647.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO145_002567"
     CDS             10939..11730
                     /locus_tag="OO145_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5467648.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO145_002568"
     CDS             11751..13037
                     /locus_tag="OO145_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5467649.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO145_002569"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO145_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5467650.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO145_002570"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO145_002570"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5467651.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO145_002571"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO145_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5467652.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO145_002572"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO145_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5467653.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO145_002573"
     CDS             complement(16985..17275)
                     /locus_tag="OO145_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5467654.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO145_002574"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO145_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5467655.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO145_002575"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO145_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5467656.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO145_002576"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO145_002576"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO145_002577"
     CDS             19137..20378
                     /locus_tag="OO145_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5467657.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4815 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAJTMX010000075        20403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTMX010000075 DAJTMX010000000
VERSION     DAJTMX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.075"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO145_002578"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO145_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5467658.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO145_002579"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO145_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5467659.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO145_002580"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO145_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO145_002581"
     CDS             1631..2341
                     /locus_tag="OO145_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5467660.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO145_002582"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO145_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5467661.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO145_002583"
     CDS             complement(3469..4119)
                     /locus_tag="OO145_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5467662.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6480
                     /locus_tag="OO145_002584"
     CDS             4282..6480
                     /locus_tag="OO145_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5467663.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="OO145_002585"
     CDS             complement(6449..6889)
                     /locus_tag="OO145_002585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5467664.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO145_002586"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO145_002586"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5467665.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="OO145_002587"
     CDS             7607..8305
                     /locus_tag="OO145_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5467666.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="OO145_002588"
     CDS             8534..9742
                     /locus_tag="OO145_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467667.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="OO145_002589"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="OO145_002589"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5467668.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="OO145_002590"
     tRNA            11849..11925
                     /locus_tag="OO145_002590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="OO145_002591"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="OO145_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5467669.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="OO145_002592"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="OO145_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5467670.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="OO145_002593"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="OO145_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5467671.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="OO145_002594"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="OO145_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5467672.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="OO145_002595"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="OO145_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5467673.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="OO145_002596"
     CDS             complement(18540..19811)
                     /locus_tag="OO145_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5467674.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="OO145_002597"
     CDS             20287..20394
                     /locus_tag="OO145_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5467675.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4891 a         5049 c         5369 g         5094 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtaac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atg
//
LOCUS       DAJTMX010000076        20309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTMX010000076 DAJTMX010000000
VERSION     DAJTMX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.076"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            56..850
                     /locus_tag="OO145_002598"
     CDS             56..850
                     /locus_tag="OO145_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5467676.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="OO145_002599"
     CDS             872..2281
                     /locus_tag="OO145_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467677.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="OO145_002600"
     CDS             2259..3038
                     /locus_tag="OO145_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5467678.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="OO145_002601"
     CDS             3035..3895
                     /locus_tag="OO145_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5467679.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="OO145_002602"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="OO145_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="OO145_002603"
     CDS             complement(4635..4895)
                     /locus_tag="OO145_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5467680.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="OO145_002604"
     CDS             complement(4886..6157)
                     /locus_tag="OO145_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5467681.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OO145_002605"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OO145_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5467682.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="OO145_002606"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="OO145_002606"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5467683.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="OO145_002607"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="OO145_002607"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5467684.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="OO145_002608"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="OO145_002608"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5467685.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="OO145_002609"
     CDS             11499..11651
                     /locus_tag="OO145_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5467686.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="OO145_002610"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="OO145_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OO145_002611"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OO145_002611"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5467687.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="OO145_002612"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="OO145_002612"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5467688.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="OO145_002613"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="OO145_002613"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5467689.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="OO145_002614"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="OO145_002614"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5467690.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="OO145_002615"
     CDS             17363..17686
                     /locus_tag="OO145_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5467691.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="OO145_002616"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="OO145_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5467692.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="OO145_002617"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="OO145_002617"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5467693.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="OO145_002618"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="OO145_002618"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5467694.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..>20309
                     /gene="truD"
                     /locus_tag="OO145_002619"
     CDS             19396..>20309
                     /gene="truD"
                     /locus_tag="OO145_002619"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5467695.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYP"
BASE COUNT         4891 a         5065 c         5517 g         4836 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgttgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccggta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atggttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatcc
//
LOCUS       DAJTMX010000077        20287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTMX010000077 DAJTMX010000000
VERSION     DAJTMX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.077"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO145_002620"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO145_002620"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5467696.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO145_002621"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO145_002621"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5467697.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO145_002622"
     CDS             complement(1728..2999)
                     /locus_tag="OO145_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467698.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="OO145_002623"
     CDS             complement(3182..4387)
                     /locus_tag="OO145_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467699.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="OO145_002624"
     CDS             complement(4487..4843)
                     /locus_tag="OO145_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5467700.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OO145_002625"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO145_002625"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO145_002626"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO145_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5467701.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OO145_002627"
     CDS             6773..9829
                     /locus_tag="OO145_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5467702.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OO145_002628"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OO145_002628"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5467703.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OO145_002629"
     CDS             10336..10524
                     /locus_tag="OO145_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5467704.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OO145_002630"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OO145_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5467705.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OO145_002631"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OO145_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5467706.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OO145_002632"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OO145_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5467707.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OO145_002633"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OO145_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5467708.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OO145_002634"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OO145_002634"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5467709.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OO145_002635"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OO145_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5467710.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OO145_002636"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OO145_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5467711.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO145_002637"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO145_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5467712.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO145_002638"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO145_002638"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5467713.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4862 c         5177 g         4932 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgacaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttc
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaacta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gtttgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    20281 atgctga
//
LOCUS       DAJTMX010000078        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTMX010000078 DAJTMX010000000
VERSION     DAJTMX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.078"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO145_002639"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO145_002639"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5467714.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO145_002640"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO145_002640"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5467715.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO145_002641"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO145_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5467716.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO145_002642"
     CDS             6038..6658
                     /locus_tag="OO145_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5467717.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO145_002643"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO145_002643"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5467718.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO145_002644"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO145_002644"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5467719.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO145_002645"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO145_002645"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5467720.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO145_002646"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO145_002646"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5467721.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO145_002647"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO145_002647"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO145_002648"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO145_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO145_002649"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO145_002649"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5467722.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO145_002650"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO145_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5467723.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO145_002651"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO145_002651"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5467724.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO145_002652"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO145_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5467725.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO145_002653"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO145_002653"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5467726.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO145_002654"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO145_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5467727.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4682 a         5506 c         5032 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTMX010000079        19375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTMX010000079 DAJTMX010000000
VERSION     DAJTMX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.079"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO145_002655"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO145_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5467728.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO145_002656"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO145_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5467729.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO145_002657"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO145_002657"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5467730.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO145_002658"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO145_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5467731.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO145_002659"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO145_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5467732.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO145_002660"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO145_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5467733.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO145_002661"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO145_002661"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5467734.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO145_002662"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO145_002662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5467735.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO145_002663"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO145_002663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5467736.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO145_002664"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO145_002664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5467737.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO145_002665"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO145_002665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5467738.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO145_002666"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO145_002666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5467739.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO145_002667"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO145_002667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5467740.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO145_002668"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO145_002668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5467741.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO145_002669"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO145_002669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5467742.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO145_002670"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO145_002670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5467743.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO145_002671"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO145_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5467744.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO145_002672"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO145_002672"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5467745.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4769 a         4855 c         5119 g         4632 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggtga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAJTMX010000080        19308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTMX010000080 DAJTMX010000000
VERSION     DAJTMX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.080"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO145_002673"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO145_002673"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5467746.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO145_002674"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO145_002674"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5467747.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO145_002675"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO145_002675"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5467748.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO145_002676"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO145_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5467749.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO145_002677"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO145_002677"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5467750.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO145_002678"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO145_002678"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5467751.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO145_002679"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO145_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5467752.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO145_002680"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO145_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5467753.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO145_002681"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO145_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5467754.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO145_002682"
     CDS             11296..11511
                     /locus_tag="OO145_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5467755.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO145_002683"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO145_002683"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5467756.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO145_002684"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO145_002684"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5467757.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO145_002685"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO145_002685"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5467758.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO145_002686"
     CDS             complement(16247..16786)
                     /locus_tag="OO145_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5467759.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO145_002687"
     CDS             complement(16802..17320)
                     /locus_tag="OO145_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5467760.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO145_002688"
     CDS             complement(17631..17822)
                     /locus_tag="OO145_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5467761.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO145_002689"
     CDS             17840..17992
                     /locus_tag="OO145_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467762.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..>19308
                     /gene="pdeF"
                     /locus_tag="OO145_002690"
     CDS             18174..>19308
                     /gene="pdeF"
                     /locus_tag="OO145_002690"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5467763.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4545 a         4701 c         5250 g         4812 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaat
//
LOCUS       DAJTMX010000081        18154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTMX010000081 DAJTMX010000000
VERSION     DAJTMX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.081"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1603)
                     /locus_tag="OO145_002691"
     CDS             complement(<1..1603)
                     /locus_tag="OO145_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR5467764.1"
                     /translation="MLSLYEKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRADSR
                     FELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDD
                     NKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNP
                     AYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLK
                     QGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGN
                     LLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEAL
                     KIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYL
                     AVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCREN
                     VQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMF
                     SEFIFCDDAKLNEIEENIWIARNIRH"
     gene            complement(1781..2692)
                     /gene="gsiD"
                     /locus_tag="OO145_002692"
     CDS             complement(1781..2692)
                     /gene="gsiD"
                     /locus_tag="OO145_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5467765.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(2695..3615)
                     /gene="gsiC"
                     /locus_tag="OO145_002693"
     CDS             complement(2695..3615)
                     /gene="gsiC"
                     /locus_tag="OO145_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5467766.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3633..5171)
                     /gene="gsiB"
                     /locus_tag="OO145_002694"
     CDS             complement(3633..5171)
                     /gene="gsiB"
                     /locus_tag="OO145_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5467767.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(5191..7062)
                     /gene="gsiA"
                     /locus_tag="OO145_002695"
     CDS             complement(5191..7062)
                     /gene="gsiA"
                     /locus_tag="OO145_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5467768.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(7049..8014)
                     /gene="iaaA"
                     /locus_tag="OO145_002696"
     CDS             complement(7049..8014)
                     /gene="iaaA"
                     /locus_tag="OO145_002696"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5467769.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            8218..9453
                     /gene="moeA"
                     /locus_tag="OO145_002697"
     CDS             8218..9453
                     /gene="moeA"
                     /locus_tag="OO145_002697"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5467770.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            9453..10202
                     /gene="moeB"
                     /locus_tag="OO145_002698"
     CDS             9453..10202
                     /gene="moeB"
                     /locus_tag="OO145_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5467771.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(10381..11043)
                     /gene="fsa"
                     /locus_tag="OO145_002699"
     CDS             complement(10381..11043)
                     /gene="fsa"
                     /locus_tag="OO145_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5467772.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            11174..12073
                     /locus_tag="OO145_002700"
     CDS             11174..12073
                     /locus_tag="OO145_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5467773.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            12079..14511
                     /locus_tag="OO145_002701"
     CDS             12079..14511
                     /locus_tag="OO145_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5467774.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            14657..15472
                     /gene="ybiV"
                     /locus_tag="OO145_002702"
     CDS             14657..15472
                     /gene="ybiV"
                     /locus_tag="OO145_002702"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5467775.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            15624..16889
                     /locus_tag="OO145_002703"
     CDS             15624..16889
                     /locus_tag="OO145_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5467776.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(17130..>18154)
                     /locus_tag="OO145_002704"
     CDS             complement(17130..>18154)
                     /locus_tag="OO145_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5467777.1"
                     /translation="IRWLEQVLNERDSTMIIISHDRHFLNMVCTHMADLDYGELRVYP
                     GNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANASKSRQATSRARQIDKI
                     KLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPLFKNLNLLLEVGEKLAV
                     LGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHEYEFENDLTVFEWMSQW
                     KQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFGKLMMQKPNILIMDEPT
                     NHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEITPERVIDFSGNYEDYL
                     RSKGIE"
BASE COUNT         4445 a         4938 c         4509 g         4262 t
ORIGIN      
        1 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
       61 cacagaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
      121 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
      181 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
      241 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
      301 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
      361 tgcgttcatc aacaccaaac accgcctcca cggcttccag accacacagt ttgatgatcc
      421 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
      481 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
      541 tcatttccgc gatcgaattt ttgatgctga taatctcttt cgcatcgcct tcgcgaattt
      601 tcagagcctc gatctgatcc gttgagtcgg gcaaaaacgc cagcgtatta accagctcgg
      661 tcatttcaga aaattgccga cggaacacac gagtggtcat gaaatatacc accagcaaca
      721 cgataataat gaacgcaaac ggtagataaa aaagtttatc tgatgcgttc aataaattac
      781 cgccaggata ataaaaatag atttcgaaca atgcattatt tggcactgct gatgtctttg
      841 caatatactc cgtatcgaca gaatcaataa tattataatt accttcgcta ttataaataa
      901 tcagtttttc acgagcgagt tgattcttgg caatgatctc atgaccttgt ttcaaaaata
      961 gaacttcatc attagcgctg atttgatcaa gatactcgtt cagataccgc cagtttaaat
     1021 cgacatgaat tttatctacc gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa
     1081 aagagataca ttttttaccc ggacgatcaa agcaattgta tgccggattc cagctgttgt
     1141 tagagtcatc tgaagccacc gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa
     1201 cccgccctga ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt
     1261 tatcattcgc cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg
     1321 agtctaaaaa tggcatcatt tccattttca gaatcacggc gcgttgaatc tcctctgtat
     1381 tttttaataa atacagtgag tcctttaatg tcagggccga acgttcaata tctttaaaga
     1441 aatagccaac gtttttctga ataagttcaa agcggctatc ggcacgtttt accgcgcgtt
     1501 ccgataccag ttgatagtta atcatgaaaa aaagaccaat aaatgacagt gctgccaata
     1561 aaaataaaat tatcagcctt atcttttcgt ataaactcag catgttaata catttaacaa
     1621 aataactatc tgaacgctgt gccgctgcaa aaatagtccc ttgctgcggc tttttaacca
     1681 aattacccga ttctacaaaa tcagcagaaa gttacaacac ttgcttaggg caataatttc
     1741 ctgtgaggtg attacccttt caagcaatat tcaaacgtaa ctatccttta attttcggat
     1801 ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc cagtacggtc agaaaaatag
     1861 ccagggccgg aaaaacagcg acatgcggcg cgataaccat atccgctcgt gcctcattga
     1921 gcattgctcc ccactctggt gtcggcggct gcgcaccgag gccgagaaat gacaggctgg
     1981 cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa caccacgata gaagagacgg
     2041 tcccaggcag gatatgacgc aataaaatgg tcatatcgct ggcaccaata ctgcgtgctg
     2101 actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc gcgcaccagg cgggcaaacg
     2161 cggggatgga aaaaatggcg actgcgataa tcacgttagc aatgccgctt cccaacaccg
     2221 caacaacagc gatcgccagt aaaatacccg ggaaggcaaa cagcacatcg caaatgcgca
     2281 tgatcagccg atcccaccag ccttcataat atccagccaa caagcccagc aacgtcccta
     2341 tcgccacacc gataaacacg gcaaacacgc ccgccgccag cgagatttgc gcaccaacca
     2401 ggacacggct gaaaatgtcg cgccccagtg aatcgacgcc aaaccagtgc tgcaaagaag
     2461 gtccgttatt cagattgtca taatcaaaat aattttcggc gtcataggga gcgatccagc
     2521 gtgcaaaaat ggccaccaca atcaataaaa taacgaataa tgcggcggtc atcgccatat
     2581 gctgacggcg aaatcgtcgc cagaattcat gccacggtgt acgtacctgg tcaggtttga
     2641 ccagtggcat ggcgtttaac accgcctgac gtcgccagtt aaatagtcgc atccttactt
     2701 gtacctgata gccgggttaa tggcggcgta aagcacatcc accactaagt tgataagaat
     2761 aaattccagc gagaaaagca gaatttccgc ctgaatcacc gggtaatcgc gcatttctac
     2821 agagtcaacg agtaagcgtc caagtccggg ccagttgaaa actttctcca ccacgataga
     2881 accaccgagc aaaaagccaa actgtaagcc catcatggtc actaccggga tcatcgcgtt
     2941 gcgtagcccg tgtttgagga caacccaggt ttcgctcacc cctttcgccc tcgcggtacg
     3001 catataatct tcgcttaaaa cgtcgacaaa tgacgcacgg gtaaagcgcg ccatcacggc
     3061 ggccactgcc gcgccgagcg tcagggaggg taaaatgtag tgctgccaac tgtctgctcc
     3121 cacagtaggc agccagccca gttcaacgga gaatacctga attaaaaaca tccccagcgc
     3181 aaatgccgga aacgagatcc ccgacaccgc aatggtcata ctcaatcgat ccggccaacg
     3241 attacgccag acggcggcga taattcccgc cgccatacca aatatcaccg cccagaccat
     3301 actggttatg gtcagccata gcgttggcat aaagcggctg gcaatctcat cggcaaccgg
     3361 acgacgcgac accatcgaca ggccaaaatc cccctgcaca gcattgctga tatagtgcca
     3421 gaactggtga tacagcggct gatccaaccc cagctgctga cgcaccagtt ctataacctg
     3481 cgcatcagct tcgggcccgg caatcaatcg cgccggatcg ccgggcagca tgtggacaaa
     3541 taaaaacacc agcaccgaga cgataaacag cgtcggaatc aaccccagta atcgtttgat
     3601 aacgtaataa agcattccac tccctgcgtt gcttattgca aatccgcgtc ttcaaagctg
     3661 aagccggtat ctggcatgat ccaaaaaccg gtcaggtttt tactgtgtgc cgacaccagt
     3721 ttttctacca ccagcgggat ccacggcgat tcttgccaga tgatatcctg cgccgcctta
     3781 tataagcggg tcttttccgc cggatcattc gttttcagtg cctgagccag gaagtcatcc
     3841 acctgtttat tgctgtaaaa cgcggtatta aacagcgtcg gtggccagtt ttgcgaggca
     3901 aacagcggcg atagtgccca gtcggcttcg ccggttgaag ccgaccagcc agtgtagaac
     3961 atccgcacgc cactctcttt ttgcccttta ccttcaactt ctgccgcccg ctgtccggca
     4021 tccatcgcgg tcacctgggc tttaatcccg acctgcgcta actgctgctg ggtaaattgc
     4081 agcactttct gcgcggtgct gtggttatgt gacgaccaca gcgtggtact gaaaccgttg
     4141 ggatatcccg cctctttcag taattcgcgc gctttcactg gatcgtaagg ccacggttta
     4201 taactttgcg cgtaggcgat acttggcggt accacaccag tagctggagt tgcatagccc
     4261 gcaaaggcaa ctttcaccag cgccggacgg ttaatggcgt aattcagcgc ctcacggacc
     4321 ttcgggttat cgaacggctt ttgcgtcacg ttcatactga tataacgctg cataattgac
     4381 ggactggcca tcaactcgat atttttgttt ttctccagca gtgtggcctg ctcgtaggga
     4441 atggggaaag caaactgcgc ttcaccggtt tgcagcattg ccgcgcgggt gttgttatcc
     4501 gccaccggac gccaggttat gctgtccagt ttgggcaatc ctggctgcca gtaacccgcg
     4561 aattttttca ccttcacaaa atcggtctga ttccaggtat ccagttcata cggtccggtt
     4621 cccaccggat gaaaaccaat ctccttgcca tatttttcca gcgctgtcgg tgaaatcatc
     4681 gcggtcgccg gatgggcaag aatattaata aacgctgaga acggctgttt gagggtaatc
     4741 tttaccgttg tcggatcgat cgcctccgtt ttggcaatat tcttatacag gttatagcgt
     4801 ttaagatgat tcgccggatc gctggcccgg tccagattcg cttttaccgc cactgcgttg
     4861 aaatcggtgc catcctggaa tttaattcct tcccgcaatt tcacggtgta agtaatgccg
     4921 tcatcggaaa cggtataact ctccgccagc acgtttttca gtttcatctc tttatccaga
     4981 ccgaacagcc cctggtaaaa cgatttcgct acggcctgag ataacgtgtc attcgcgtca
     5041 tacggatcga gcgtggtgaa attcgatcct accgccacca ccacatcttt ggcagcgaat
     5101 gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca ctaacccact acggtgtaca
     5161 gctcttgcca ttttattctc cgcctgaatg ttatctacgc atgaatgcgt attctgattg
     5221 tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac gagacggctg ccacctcttc
     5281 gccacgcaga tgaatattgc tgggaagatc gtccgacagc agtacacgct gcggtcgttg
     5341 tcgggacggt tcagcgaccg gaactgccgc cagtaattta cgcgtataag gatgctgcgg
     5401 gttttcgaag accgcgcacc gtgaaccaat ttcaacaatt tgcccgagat acatcaccgc
     5461 cacacgatga ctaatccgct ctaccacagc catatcgtgg gagataaaca gatacgcaat
     5521 gccgaaatca cgctggagat cgagcaacaa gttgataatc tgcccgcgaa tagaaacatc
     5581 cagcgccgaa acggcttcgt cggcaatgat cacttttgga ttcaatgcca acgcgcgagc
     5641 aatgcagatg cgctggcgct gaccgccgga aaactcatgc gggtaacgcc aggcatgttc
     5701 aggtaacagg cccacgcgct ccagcaacca cgcaacgcgt gcagccgcgt ctttacctgg
     5761 caataaaccg tgtacacgca gcggttcgat aatcgaatca ccgatggtct gacgtgggtc
     5821 cagcgaagcg taagggtcct gaaaaataaa ctgaatatcc cggcgcaatg cctgaagttt
     5881 gccaggtgac aaggtatcga ttcgctgacc gttaaagata atttcgccgc cctgcgattc
     5941 gaccaggcgc agcaacgccc gcccggtagt ggatttaccg ctgccagact cgcccaccag
     6001 cgatagcgtt tcgccaggcc agagatcaaa actgactttc tcaacggcat gcacttcccg
     6061 cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg gtgacaagat tacgcacccg
     6121 taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg gggggttcct gtttcgctgg
     6181 atgttcaagc gatatcaatg ggaaacgtcg gggataatct aaccctttca tcgcaccaag
     6241 ttgcggaaca gcagctaaca gcgcacgggt gtaaggatgt tgcggtgcat gaaaaatctg
     6301 ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc accagtaccc gatcggcaat
     6361 ctctgccacc acgcccatat cgtgagtgat aaagataacg cccatcgaca tctctttttg
     6421 caatactttg attaattgca ggatctgcgc ctgaatagtg acatccagcg cggtggttgg
     6481 ctcatcggca atcagcaccg ccgggcggca tgacagcgcc atcgcaatca tcactcgctg
     6541 gcgcatcccg ccagagagtt gatgcgaata acgtgaaaga atggtttgtg cctccggaat
     6601 gcgtacctga tccagcatcc gcttcgcctc gaccatcgct tcttcacgac tggcgttctg
     6661 atgcagacga attgattcgg caatctgttc acccacagta aatactgggt tcagcgatgt
     6721 catcggctcc tgaaatatca tcgccatatc cgcaccgcgc acatggcgca tttgtgcagc
     6781 gctctgctcg ctaagttcaa tcacatcacg actgcgccgc cgcaacagca ttttatcgca
     6841 ctgtactaaa ccgcccgcct gttccaacag acgcatcaat gccagcgcag tcactgactt
     6901 accggagccg gattcgccta caattgccag cgtctcaccg cgttgcagac taaaagagag
     6961 attgcggacc gcagctattt tctgctggtc ctgcataaag gcaatattaa gattttcaac
     7021 cgccagcaca ttaccggcat caagttcatc actgtgtggc aacggtgtcc cctttttcac
     7081 ggtagatacc ggtggttggc gtatcgcctg cgtagcccca ggcgcgatac attccttcgg
     7141 tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc gattaagcca ccgctaccgc
     7201 caagcgcagg gagtttttcc attactaccc gctcgcaggc ttccgcgaga cttaatccgc
     7261 cgtaatccat taacgcggcg atgtcatatg ccgccagcgc gcggatgaag acttcgcctg
     7321 tgccggtaca agaaaccgcc acactggcgt tattggcgta gcatccggca cccactaagg
     7381 gactatcgcc aactcgtccg ggtaatttat tggtcattcc gcctgtggac gtggctgccg
     7441 ccagattgcc gtctaaatcc aacgccacgg cccccacggt gcccattttc tgtttttcat
     7501 ccagtggcgc accgctatgg tcgaggactg ttgccccttc ctcgcgcgct gccagtagtt
     7561 gttcataacg caaaggcgtg gagaaaatct ccggtgagac gcgctccatg ccatgagcaa
     7621 acgcaaaatt ttctgcccct tcgccaatca tcatcacatg cgggctttgc tccatcacca
     7681 gccgggcggc aagaaccgga ttacgcagat gactaacgcc cgccaccgca ccggctttca
     7741 gggtgttacc atccatcaca caggcgtcca gttcatgggt ttcatcacgc gtaaagacag
     7801 cgccaattcc ggcgttaaac agtggacact cttccagcag acgcaccgct tccgtcacca
     7861 catccagcgc actttcgccg gcttccagca ttttctgccc ggtttcaaca atggcagaca
     7921 acgcctcgat gtagcgtaat tcctgttgca gactcatctg cgcgcggcta attgcacctg
     7981 cgccaccatg aattgcaatg actgctttgc ccatagtatt cgtccattaa gggtggctcg
     8041 ccacttttgc tataaatatc agaattttta tgaattactc attcaatttt tgaatataga
     8101 aagatggata tggcatgtaa aggcaggagc caacgctacg cccgaggtgg catacagtct
     8161 atggcgtttt tctgacataa taggcaaatt cgattttgcc tccgcaggag tgttttcatg
     8221 gaatttacca ccggattgat gtcgctcgac accgcgctta atgagatgct ttctcgcgtt
     8281 accccactga ccacccagga aacgctgcca ctggttcagt gttttggtcg tattctggcg
     8341 agcgatgtcg tttcgccact ggatgtcccg gggtttgata actccgctat ggacggctac
     8401 gcggtgcgtt tagccgatat tgcctccggg caaccgctgc ccgttgccgg taaatccttt
     8461 gccggtcagc cataccatgg tgaatggcct gcgggtacct gcattcgtat tatgaccggt
     8521 gcgccggtgc cggaaggctg cgaagcggtg gtgatgcagg agcagactga acaaacggac
     8581 aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa atattcgccg tcgcggtgaa
     8641 gatatctctg caggtgcggt tgttttcccg gcgggaactc gcctgactac cgcagagctg
     8701 ccagtaattg cttcactggg gattgccgaa gttccggtga ttcgtaaagt gcgtgtagcg
     8761 cttttttcta ccggtgatga actccagttg cccggtcagc cgctgggcga cggccaaatc
     8821 tacgacacca accgtctcgc cgtacacctg atgttagaac agctgggttg cgaggtaatc
     8881 aacttaggga ttatccgcga cgatccacat gccctgcgcg ccgcatttat tgaagccgac
     8941 agccaggcgg atgtagtgat cagttccggc ggtgtttcag tgggtgaagc ggattacacc
     9001 aaaatgattc ttgaagagct gggggagatc gccttctgga agctggcgat taaaccaggt
     9061 aaaccgttcg cgttcggtaa actcagcaat agctggttct gcggcctgcc gggcaacccg
     9121 gtttcagcga cgctgacctt ctatcaactg gtacagcctt tgctggcaaa actaagcggc
     9181 aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca cagcgtcccg cctgaaaaaa
     9241 acgccaggac gtcttgattt ccagcgcggc gtgctgcaac gcaacgccga cggtgaactt
     9301 gaagtgacga ccaccggaca tcagggttca catatattta gctcctttag tctcggcaac
     9361 tgctttatcg tgctggaacg cgatcgcggc aatgtggacg tgcgcgaatg ggtggaagta
     9421 gaaccgttta acgcgttgtt cggaggcctg taatggcgga actcagcgat caggagatgc
     9481 tgcgctacaa ccggcaaatc attctgcgcg gttttgattt tgacggtcag gaggcgctaa
     9541 aagattctcg tgtgctggta gtcggcctcg gtggactcgg ctgcgcagct tcgcagtatc
     9601 tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt cgacacggtt tcgctctcga
     9661 atctgcaacg ccagacactg cacagtgatg ccacggtcgg gcaaccgaag gtggaatccg
     9721 cccgtgacgc cctgacgcgg atcaacccac atatcgcgat tacgccagtc aatgcactgc
     9781 tagatgacgc agaacttgca gcattgattg ctgaacacga tctggtgctc gactgtacgg
     9841 ataacgttgc ggtacgtaat caactgaacg caggctgttt tgccgcgaag gtgccgctgg
     9901 tttccggcgc ggcaattcgt atggaagggc aaatcacggt cttcacttat caggacggcg
     9961 aaccgtgcta tcgctgcctt agccgtttgt ttggcgagaa cgcgttaacc tgcgtagaag
    10021 caggcgtgat ggcaccgttg ataggcgtaa ttggttcgtt gcaggcaatg gaagcgatca
    10081 aactgctggc aggttatggc aaaccggcca gcgggaaaat cgtcatgtac gatgcgatga
    10141 cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg gtgtgaggtg tgcgggcagt
    10201 aattgccacc tccaagcctg ataagacgcg acaagcgtcg catcaggcat ttgctccaga
    10261 cgccagatgc ggcgtgaacg ccttatctgg cctacggttc gggcacagat ttccaagcgc
    10321 ggcaggcatc ggtgcactcc gccacgtcat cagcaattta tgaggatgca gaacgtccgg
    10381 ttaaatcgac gttctgccaa acgctccctg ccagtcctgc tcaaacttcg tcacagcggc
    10441 ttccaccgcc ggatagctaa tcatctgttg tgccacatcc agtggcagag taattgattc
    10501 acatccggcc agtaagcagt ccagcgcctg acgcggggtt ttgaaactcg ctgccagcac
    10561 tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag tcggtcacag tctgaatgcc
    10621 gctaccgccc tgagcatcaa tacgattaac gtaaggcgca acatattccg cacctgccag
    10681 cgccgacagc agcccttgtg ccgcgccata taccgcggtt cccagcgtcg gaatcccttc
    10741 cgcttttaac atcttaatcg ctgccagccc ctcggctgtc accggaactt tcaccacgat
    10801 atccgcaata atagaacgca gcttaagcgc gtcattcacc atcccttcag cagtggtagc
    10861 cattacctgg gcaaacagac gcccctgacc gcccatcgct tcatgaagtt gcggaagcac
    10921 aacatccagc ggttttttac ccgcggcgat aatgcttggg ttagtggtca cacccgccag
    10981 cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct gaagtatcca gatacagttc
    11041 cataaccatc ctcaaaatgt actttttgtc tgaatgtaga ctatcacggg aaaactctct
    11101 gaacagttga cgcacatcaa tctaactttc attcgaaagt attttaatgt ttgaacgaaa
    11161 cagagaggca actatgattt tcaatattca gcgctactcg acccatgatg gccccggtat
    11221 ccgcacggtc gtatttctta aaggctgttc gctgggctgc cgctggtgtc agaacccgga
    11281 aagccgcgcc cgtacgcagg atctgctgta tgacgcacga ctgtgtctgg aaggctgcga
    11341 gctgtgcgct aaggccgcgc cggaagtgat tgagcgcgcg ctgaatggtt tgcttattca
    11401 tcgggaaaag ttaaccccgg agcatctgac ggcgttaacc gactgctgtc cgacacaggc
    11461 gttaaccgtg tgtggtgaag tgaaaagcgt tgaggagatc atggcgaccg ttctgcgcga
    11521 taaaccgttt tacgatcgca gcggcggcgg cttaacgctt tcgggtggtg aacccttcat
    11581 gcagccggaa atggcgacgg cgctgctgca agcctgccac gaggcaggca ttcatactgc
    11641 ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc ccttctctgc cctatatcga
    11701 tctgtttctt gccgatttaa aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg
    11761 taacgccgcc agggtgctgg ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat
    11821 tatccgcgtg ccgctgattc agggctttaa tgccgacgaa acctctgtaa aagccattac
    11881 cgattttgcc gccgacgagc tgcacgttgg cgaaattcat tttctgccct accacacgct
    11941 gggcatcaac aaatatcact tacttaatct gccctatgac gccccggaaa aaccgcttga
    12001 tgcgccagag ctgctcgatt ttgcccagca gtatgcctgc cagaaagggt taaccgcgac
    12061 cttacgagga taacaaccat gaccacactg aaactggaca cgctcagcga ccgcattaaa
    12121 gcgcacaaaa atgcgctggt gcatattgtg aagccgccag tctgtaccga gcgcgcgcag
    12181 cactataccg agatgtatca acaacatctc gataagccga tcccggtacg tcgcgcgctg
    12241 gcactggcgc atcacctggc gaatcgcacc atctggatca aacacgatga gttgatcatt
    12301 ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg
    12361 atcgaaaaag agattgatga tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa
    12421 gagaacaaac gtgttctgca tgaagtgtgc ccgtggtggc gcggtcagac ggtacaggat
    12481 cgctgctatg gcatgtttac cgatgagcaa aaaggtctgc tggcgaccgg catcattaaa
    12541 gcggaaggca atatgacctc cggcgatgcg cacctggcgg tcaatttccc gctgctgctg
    12601 gaaaaagggc ttgatggtct gcgtgagaaa gtagcggaac gtcgctcgcg catcaatctg
    12661 acggtgctgg aagatttgca cggcgaacag ttcctgaaag cgattgatat catgctggtg
    12721 gcagtcagtg aacacattga acgtttcgct gccctggcgc gtgcaatggc cgcgaccgaa
    12781 acccgcgaaa gccgtcgcga tgaactgctg gcgatggcag aaaactgcga tcttatcgcc
    12841 caccagccgc cgcagacttt ctggcaggcg ctgcaactgt gttacttcat ccagttgatt
    12901 ttgcagattg aatctaacgg tcactcggtg tcgtttggtc gtatggatca gtatctctac
    12961 ccgtactatc gccgcgacgt tgaactgaac cagacgctgg atcgcgaaca cgccatcgaa
    13021 ttgctgcata gctgctggtt gaaattgctg gaagtgaaca aaatccgctc cggctcccac
    13081 tcaaaagcct ctgcgggaag tccgctgtat cagaacgtca ccattggcgg acaaaatctg
    13141 gttgatggtc aaccaatgga cgcggtgaat ccactctctt acgcgatcct cgaatcctgc
    13201 ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt accatgcagg aatgagtaac
    13261 gatttcctcg acgcctgcgt acatgtgatc cgctgcggct tcgggatgcc ggcattcaac
    13321 aacgacgaaa tcgtgatccc ggaatttatt aaactcggta ttgaaccgca ggacgcttat
    13381 gactacgcag cgattggttg tatagaaacc gccgtcggtg gcaaatgggg ctatcgctgt
    13441 accggcatga gctttatcaa cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt
    13501 gatgccacca gcggcaaagt gttcctgcca caagaaaaag cgttgtcggc aggtaacttc
    13561 aacaacttcg atgaagtgat ggacgcgtgg gatacgcaaa tccgttacta cacgcgtaaa
    13621 tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag agaacgtgca cgatattctc
    13681 tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg
    13741 aaatatgact gggtttctgg cctgcaggtc ggtattgcca acctcggcaa cagcctggcg
    13801 gcagtgaaga aactggtgtt tgaacagggc gcgattggtc agcaacagct ggctgccgca
    13861 ctggcggatg acttcgatgg cctgactcat gagcaactgc gtcagcggct gattaacggt
    13921 gcgccgaagt acggcaacga cgatgatacc gtcgatacgc tgctggctcg cgcttatcag
    13981 acctatatcg acgaactgaa acagtaccat aacccacgct acggtcgtgg tccggttggc
    14041 ggcaactatt acgcgggtac gtcgtctatc tccgctaacg taccgtttgg cgcgcaaact
    14101 atggcaacgc cggacgggcg taaagcccac accccgctgg cagaaggcgc tagtccggcc
    14161 tctggtactg accatctcgg ccctactgcg gtcattggct cagtgggtaa actgcctacg
    14221 gcagcgattc tcggcggcgt gttgctcaac cagaaactga atccggcaac gctggagaac
    14281 gaatctgaca agcagaaact gatgatcctg ctgcgtacct tctttgaagt gcataaaggc
    14341 tggcatattc agtacaacat cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccg
    14401 gatcagtacc gcgatctggt agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc
    14461 tctccagacg ctcaggacga tatcatcgcc cgtactgaac atatgctgta atctcttcgg
    14521 cccggctgcg gcgtcgccgg gctaaatcat tcacatcatc aatttcatcc ttactttcat
    14581 tcgaaatata atttgtgctc tgcgtcacat tgttattaca ctgttacggt ttcattttcg
    14641 agccaggagc acacctatga gcgtaaaagt tatcgtcaca gatatggacg gtacttttct
    14701 taacgacgcc aaaacgtaca accaaccacg ttttatggcg caatatcagg aactgaaaaa
    14761 gcgcggcatt aagttcgttg tcgccagcgg taatcagtat taccagctta tttcgttctt
    14821 tcctgagcta aaggatgaga tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca
    14881 tggcaagcaa ctgttccacg gcgaactgac ccgacatgaa tcgcggattg ttattggcga
    14941 gttgctaaaa gataagcaac tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag
    15001 cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa cactaccatc gcctgaaacc
    15061 tgtaaaagat tatcaggaga ttgacgacgt actgttcaag ttttcgctca acctgccgga
    15121 tgaacaaatc ccgttagtga tcgacaaact acacatagcg ctggatggca ttatgaaacc
    15181 cgtcaccagt ggttttggct ttatcgacct gattattccc ggtctacata aagcaaacgg
    15241 tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga
    15301 cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc
    15361 tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc
    15421 gctgaatgtg attcaggctg tgctggataa cacatcccct tttaacagct gacctctcta
    15481 ccggagccat gcgctccggt tttcccttct ttttacatcc tgtacatcac atcaaatcat
    15541 taaacttgca ttaacttatt tataacttgt attgtcacat gcaaattagc atagtttcga
    15601 atgaaagtta atgcaaggtg gttatggctt ctacttttac cagcgacaca ttgcctgccg
    15661 atcacaaagc agctatccgt cagatgaagc acgcgctgcg ggcgcagctt ggcgatgtcc
    15721 agcagatctt taatcagcta agcgatgaca ttgccacgcg agtggctgaa atcaacgcac
    15781 tcaaagcaca gggcgatgcc gtctggccgg tgctgtctta tgccgatatc aaagcaggtc
    15841 atgttactgc agagcagcgc gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc
    15901 atttcccccg cgaacaagcg ctaggctggg atcagtcgat gctggactat ctgaaccgca
    15961 accgctttga cgaggtctac aaaggccccg gcgataattt cttcgggacg ctcagcgctt
    16021 cacgtcccga gatttacccc atctactggt cgcaggcgca aatgcaggcc cgccagagtg
    16081 aagaaatggc gaatgcgcag tcgtttctca atcgtctgtg gacatttgaa agtgatggaa
    16141 agcaatggtt taacccggat gtgagcgtca tctaccctga ccgtatccgc cgccttccgc
    16201 ccggaacgac ctccaaaggt cttggagcgc ataccgactc cggggcgctg gaacgctggc
    16261 tgcttccagc gtatcagcac gttttcgcca acgtctttaa tggcaatctg gcgaaatacg
    16321 atccctggca tgcggcacat cgtacggaag ttgaagagta cacggtggac aacaccacca
    16381 aatgttccgt gtttcggaca ttccagggct ggactgcgct ctcggatatg ctgcctggtc
    16441 aggggttgct gcacgtcgtg cccattcctg aagccatggc gtacgtactg ttacgtccgc
    16501 tgcttgatga tgtgccggag gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg
    16561 tatcagagca atggcatcca ctgttaattg aggcgttaac cagcattcca aaactcgagg
    16621 ccggagactc cgtctggtgg cactgcgacg tcatccattc cgttgccccc gttgaaaatc
    16681 aacagggttg gggcaacgtg atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg
    16741 cctacgcgca caaggtgaag gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc
    16801 gcgaggacta tgaaacaaac tgggaaggac gctttacgct tgccgacctc aacattcacg
    16861 gtaagcgagc gttgggcatg gatgtttgag agttaatgga agtgaattga tgtgggatta
    16921 agaaaatcga ttcgacgtta ctcagattgc tgacaagtgc gctttcttaa tgcctgatgc
    16981 ggcgttaacg ccttatccgg cctacaaagt cctgcagatt caatatatta caggtactgc
    17041 gtaggcctga taagcgtagc gcatcaggct attttgcatt tgtcatcagc cgtcgtcacc
    17101 ccgacagtat cggggtgacg gataatcact tactcgatcc ctttactacg caggtaatct
    17161 tcgtaattac cgctaaagtc gatcacgcgt tccggggtga tttccagaat gcgggtcgcc
    17221 agggagctta cgaactcacg gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc
    17281 agcgccatgt tcagtgactc gatggattcc atatccaggt ggttggtcgg ttcgtccatg
    17341 atcagaatgt tcggcttctg catcattaac ttaccaaaca gcatccgccc tttttcccca
    17401 ccggaaagca ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga
    17461 atgctgcgta ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac
    17521 acggtcagat cattttcaaa ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg
    17581 ttttcagacc attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc
    17641 agcgttgatt taccgacgcc gttggtaccc agtaccgcaa gtttttcacc gacctccagc
    17701 agcaggttga gatttttaaa cagtggaccg ttatcaaacc ctttggtcag accttccact
    17761 tccagcgcgt tacggaacag tttcttatcc tgttcaaaac ggataaacgg gttctgacgg
    17821 ctggaggctt tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc
    17881 tggcgagatt tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc
    17941 tgcgctttct tcttggcgtt atcggccagc agacgttcac gcgcttgggt cgccgccgtc
    18001 atgtactcat cgtagttacc cggataaacg cgcagctcgc cgtaatccag atccgccatg
    18061 tgggtacaga ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca
    18121 cgctcgttca gcacctgttc cagccagcga atgg
//
LOCUS       DAJTMX010000082        18045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTMX010000082 DAJTMX010000000
VERSION     DAJTMX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.082"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            441..1124
                     /locus_tag="OO145_002705"
     CDS             441..1124
                     /locus_tag="OO145_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5467778.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1168..5830
                     /locus_tag="OO145_002706"
                     /pseudo
     CDS             1168..5830
                     /locus_tag="OO145_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6198..7853
                     /gene="lldP"
                     /locus_tag="OO145_002707"
     CDS             6198..7853
                     /gene="lldP"
                     /locus_tag="OO145_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5467779.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7853..8629
                     /gene="lldR"
                     /locus_tag="OO145_002708"
     CDS             7853..8629
                     /gene="lldR"
                     /locus_tag="OO145_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5467780.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8626..9816
                     /gene="lldD"
                     /locus_tag="OO145_002709"
     CDS             8626..9816
                     /gene="lldD"
                     /locus_tag="OO145_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5467781.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10012..10485
                     /gene="trmL"
                     /locus_tag="OO145_002710"
     CDS             10012..10485
                     /gene="trmL"
                     /locus_tag="OO145_002710"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5467782.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="OO145_002711"
     CDS             complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="OO145_002711"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5467783.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="OO145_002712"
     CDS             complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="OO145_002712"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5467784.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12458..12925)
                     /gene="secB"
                     /locus_tag="OO145_002713"
     CDS             complement(12458..12925)
                     /gene="secB"
                     /locus_tag="OO145_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5467785.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="OO145_002714"
     CDS             complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="OO145_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5467786.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13381..13812)
                     /locus_tag="OO145_002715"
     CDS             complement(13381..13812)
                     /locus_tag="OO145_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5467787.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14057..15601
                     /gene="gpmM"
                     /locus_tag="OO145_002716"
                     /gene_synonym="pgmI"
     CDS             14057..15601
                     /gene="gpmM"
                     /locus_tag="OO145_002716"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5467788.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15611..16894
                     /gene="envC"
                     /locus_tag="OO145_002717"
     CDS             15611..16894
                     /gene="envC"
                     /locus_tag="OO145_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5467789.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16898..17857
                     /locus_tag="OO145_002718"
     CDS             16898..17857
                     /locus_tag="OO145_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5467790.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17844..18045)
                     /locus_tag="OO145_002719"
                     /pseudo
     CDS             complement(17844..18045)
                     /locus_tag="OO145_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4662 c         4674 g         4164 t
ORIGIN      
        1 actcccttta cttcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata
       61 gccatctata aacaataaaa atatatagaa gatatcattt gttaattaac aagcatttac
      121 gtctcttttt ttcggattta attgtgaaat tttaatcatc atattcacaa aatcaattat
      181 ctgtgaaatt tttcccagac atggtttctt tcatatttga catttattaa taatcatcat
      241 tagaatccac gaaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
      301 catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt
      361 aaagcaacat agctcaccaa taatacaatg gacaactcta ccggcattga gcgctggtcg
      421 gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      481 cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      541 tactgaaaca acaagaagaa cataataata aaattgtgct acttgaaaaa caacaagcac
      601 agctaaagag tcaactcgaa actattcaaa aacaacaaac tggcataata aacagtacaa
      661 agactttaac tcacgtaatt aaatccgtta aagaccaaca aaatactttt atttttaccg
      721 agtttaaccc agcaaaaacg aagtatttca tcctgaacaa cggttcggtg gctttagcgg
      781 gtcgagtgtt atccattgac gccacagaga atggtagtgt tattcatatc tcactggtca
      841 acttattaag tacacctatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac
      901 ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat
      961 ccacattgaa attattacca ggtcaatggc aagacattaa tttgaccctg aagggtgtat
     1021 cgcccaataa cctgggatat ctgaagttag ccatcaacat ggaaaatatt cagttcgaca
     1081 atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct
     1141 tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct
     1201 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg
     1261 cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca
     1321 ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc
     1381 gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt
     1441 tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg
     1501 tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa
     1561 ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc
     1621 ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa
     1681 gcggaaggag caaacgccat tgccctgggg cgtagcagtg tagctagcgg tactgacagc
     1741 ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat aggtgctgag
     1801 accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat
     1861 agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat
     1921 ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc
     1981 gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta
     2041 ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc
     2101 aacggtatta actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc
     2161 tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag
     2221 tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca
     2281 aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac
     2341 ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga
     2401 aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag
     2461 cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga
     2521 tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccaccaa
     2581 tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga
     2641 aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt
     2701 taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac
     2761 caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc
     2821 cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc
     2881 gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag
     2941 caaaataacc aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg
     3001 ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa
     3061 caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa
     3121 atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat
     3181 caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca
     3241 gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac
     3301 tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg
     3361 cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg
     3421 ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat
     3481 cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca
     3541 actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc
     3601 agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg
     3661 tgatgccctg aatgctatcg ataccactcc tgacgacgct ctgctctggg atgcggacgc
     3721 cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac
     3781 taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact
     3841 ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga
     3901 cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga
     3961 cgccctggat gcgcttgatg ataacgccct gctgtgggat aagactgcca atggcggtgc
     4021 tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg
     4081 cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat
     4141 gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta
     4201 tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt
     4261 taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc
     4321 tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc
     4381 gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt
     4441 aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt
     4501 gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc
     4561 ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc
     4621 aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc
     4681 gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa
     4741 cactctggtt ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa
     4801 ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg
     4861 ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag
     4921 tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt
     4981 taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac
     5041 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
     5101 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
     5161 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
     5221 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
     5281 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg ccaccgatgc
     5341 ggttaacgtg tcgcagctga aatcttctga agcaggcgga gtgcgctacg acaccaaagc
     5401 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
     5461 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
     5521 gcaaagcgcg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
     5581 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
     5641 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
     5701 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
     5761 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
     5821 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
     5881 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
     5941 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
     6001 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     6061 tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
     6121 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     6181 actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     6241 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     6301 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     6361 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     6421 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     6481 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     6541 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     6601 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     6661 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     6721 ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg
     6781 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     6841 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc ggctcgtttg
     6901 ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt
     6961 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     7021 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     7081 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     7141 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     7201 tgatcaacat tccggtgccg tacctcaata aactggttgc ccgtatgcca ccagtggtca
     7261 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     7321 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     7381 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     7441 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     7501 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     7561 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     7621 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     7681 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     7741 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     7801 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     7861 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     7921 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     7981 atcacgtaat tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag
     8041 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     8101 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     8161 agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg
     8221 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     8281 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     8341 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     8401 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     8461 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     8521 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     8581 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     8641 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     8701 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     8761 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     8821 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     8881 cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc
     8941 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     9001 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     9061 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     9121 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     9181 gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt
     9241 aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg
     9301 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     9361 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     9421 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     9481 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     9541 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     9601 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     9661 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     9721 tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg
     9781 gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac
     9841 cctctccctc agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga
     9901 caccgcactc tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc
     9961 cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
    10021 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    10081 aataccggct ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga cgacaagcgc
    10141 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    10201 cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    10261 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    10321 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg
    10381 atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa
    10441 gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga
    10501 tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa
    10561 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
    10621 gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca
    10681 gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt
    10741 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
    10801 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg
    10861 tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg
    10921 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
    10981 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
    11041 ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg
    11101 cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcaa
    11161 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    11221 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    11281 gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    11341 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
    11401 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtgactgct gcgctcgtcc
    11461 ttgcgtgcac gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    11521 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    11581 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
    11641 acatccatgc cctgaccgag catcatacca aaacggcggt tacgcgactg gttgtcggta
    11701 caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc
    11761 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
    11821 ttcgcaccaa aaccgatacc gtcagacatc cccgcgccaa tggcaatgac gtttttcacc
    11881 gcgccgccaa gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa acttttgccg
    11941 cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata
    12001 gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    12061 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct
    12121 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    12181 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg actggctgcc
    12241 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    12301 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    12361 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    12421 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    12481 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    12541 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    12601 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    12661 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    12721 ccaaggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg
    12781 aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    12841 gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca
    12901 tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    12961 gcgcaatgta ataccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    13021 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
    13081 aaccgtggtg caaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat
    13141 cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    13201 cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc tgttttttta
    13261 ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca
    13321 ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa
    13381 ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag
    13441 cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat
    13501 gccagaaccg tctaccacga taaccgattt gtctttgtgc ttctcaagct caccaacatt
    13561 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
    13621 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
    13681 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
    13741 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
    13801 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
    13861 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
    13921 cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga
    13981 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
    14041 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
    14101 ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
    14161 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
    14221 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
    14281 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
    14341 taatccagtg ctggctggcg cggtacataa agcgaaaaac gcaggtaaag cagtacacat
    14401 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
    14461 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
    14521 cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc
    14581 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
    14641 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca
    14701 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
    14761 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
    14821 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt
    14881 gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
    14941 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
    15001 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
    15061 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
    15121 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
    15181 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
    15241 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
    15301 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
    15361 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
    15421 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
    15481 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
    15541 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
    15601 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
    15661 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
    15721 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
    15781 aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
    15841 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
    15901 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
    15961 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
    16021 gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
    16081 ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac
    16141 gcgtgaagaa gtcaccatgc agcatgctga actggaagag aaacaaagcg agcaacaaac
    16201 gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa
    16261 aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact
    16321 gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg cggcgaaagc
    16381 gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga aagaagcgac
    16441 gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
    16501 tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg
    16561 ctatggcgag cagctacagg gtgaattacg ctggaaaggg atggttatcg gtgcctctga
    16621 aggtactgaa gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact ggctgcaagg
    16681 ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa
    16741 tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt
    16801 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
    16861 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
    16921 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
    16981 cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccttc
    17041 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
    17101 gcataacagc ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc
    17161 gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc gcattattcg
    17221 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat
    17281 gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta
    17341 cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg
    17401 cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg
    17461 tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
    17521 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
    17581 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc
    17641 gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa
    17701 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
    17761 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
    17821 gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
    17881 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
    17941 ccgaccttca cgctgcgcac attggtaatc aggcgcttat acatacccgg aagtaaatat
    18001 ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcg
//
LOCUS       DAJTMX010000083        17711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTMX010000083 DAJTMX010000000
VERSION     DAJTMX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.083"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            131..574
                     /locus_tag="OO145_002720"
     CDS             131..574
                     /locus_tag="OO145_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5467791.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="OO145_002721"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="OO145_002721"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5467792.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO145_002722"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO145_002722"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5467793.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO145_002723"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO145_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5467794.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO145_002724"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO145_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5467795.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO145_002725"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO145_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5467796.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="OO145_002726"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="OO145_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5467797.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="OO145_002727"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="OO145_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5467798.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="OO145_002728"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="OO145_002728"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5467799.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="OO145_002729"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="OO145_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5467800.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="OO145_002730"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="OO145_002730"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5467801.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="OO145_002731"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="OO145_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5467802.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="OO145_002732"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="OO145_002732"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5467803.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO145_002733"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO145_002733"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5467804.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="OO145_002734"
     CDS             13934..14314
                     /locus_tag="OO145_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5467805.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="OO145_002735"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="OO145_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5467806.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO145_002736"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO145_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5467807.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO145_002737"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO145_002737"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5467808.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="OO145_002738"
     tRNA            complement(17415..17490)
                     /locus_tag="OO145_002738"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="OO145_002739"
     tRNA            complement(17494..17569)
                     /locus_tag="OO145_002739"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="OO145_002740"
     tRNA            complement(17621..17696)
                     /locus_tag="OO145_002740"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
BASE COUNT         4515 a         4625 c         4404 g         4167 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg a
//
LOCUS       DAJTMX010000084        17647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTMX010000084 DAJTMX010000000
VERSION     DAJTMX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.084"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..674)
                     /gene="chbR"
                     /locus_tag="OO145_002741"
                     /pseudo
     CDS             complement(1..674)
                     /gene="chbR"
                     /locus_tag="OO145_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(682..1032)
                     /gene="chbA"
                     /locus_tag="OO145_002742"
     CDS             complement(682..1032)
                     /gene="chbA"
                     /locus_tag="OO145_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5467809.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="OO145_002743"
                     /pseudo
     CDS             complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="OO145_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="OO145_002744"
     CDS             complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="OO145_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5467810.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="OO145_002745"
     CDS             complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="OO145_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5467811.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3683..4510
                     /gene="nadE"
                     /locus_tag="OO145_002746"
     CDS             3683..4510
                     /gene="nadE"
                     /locus_tag="OO145_002746"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5467812.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4740..5627
                     /gene="cho"
                     /locus_tag="OO145_002747"
     CDS             4740..5627
                     /gene="cho"
                     /locus_tag="OO145_002747"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5467813.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5587..6162)
                     /gene="ves"
                     /locus_tag="OO145_002748"
     CDS             complement(5587..6162)
                     /gene="ves"
                     /locus_tag="OO145_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5467814.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6365..6850)
                     /gene="spy"
                     /locus_tag="OO145_002749"
     CDS             complement(6365..6850)
                     /gene="spy"
                     /locus_tag="OO145_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5467815.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7180..8148)
                     /gene="astE"
                     /locus_tag="OO145_002750"
     CDS             complement(7180..8148)
                     /gene="astE"
                     /locus_tag="OO145_002750"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5467816.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8141..9474)
                     /gene="astB"
                     /locus_tag="OO145_002751"
                     /pseudo
     CDS             complement(8141..9474)
                     /gene="astB"
                     /locus_tag="OO145_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9471..10949)
                     /gene="astD"
                     /locus_tag="OO145_002752"
     CDS             complement(9471..10949)
                     /gene="astD"
                     /locus_tag="OO145_002752"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5467817.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10946..11980)
                     /gene="astA"
                     /locus_tag="OO145_002753"
     CDS             complement(10946..11980)
                     /gene="astA"
                     /locus_tag="OO145_002753"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5467818.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11977..13197)
                     /gene="astC"
                     /locus_tag="OO145_002754"
                     /pseudo
     CDS             complement(11977..13197)
                     /gene="astC"
                     /locus_tag="OO145_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13643..14449
                     /gene="xthA"
                     /locus_tag="OO145_002755"
     CDS             13643..14449
                     /gene="xthA"
                     /locus_tag="OO145_002755"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5467819.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14616..15326
                     /locus_tag="OO145_002756"
     CDS             14616..15326
                     /locus_tag="OO145_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5467820.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15331..16008
                     /gene="ydjY"
                     /locus_tag="OO145_002757"
     CDS             15331..16008
                     /gene="ydjY"
                     /locus_tag="OO145_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5467821.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16023..16730
                     /locus_tag="OO145_002758"
     CDS             16023..16730
                     /locus_tag="OO145_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5467822.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16730..17278
                     /locus_tag="OO145_002759"
     CDS             16730..17278
                     /locus_tag="OO145_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5467823.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17288..17614
                     /locus_tag="OO145_002760"
                     /pseudo
     CDS             17288..17614
                     /locus_tag="OO145_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4366 a         4687 c         4307 g         4287 t
ORIGIN      
        1 atctcattaa taatctgcat tggtgttttg ccataatatc gttgagtcgc tcgcgtcaaa
       61 tattcctgtg atttggctga caacgtcacc atattctcca gcgccgattc actaaactgc
      121 tctttatcat gcatcttttc taccgtactt ttcagccact acggtacatc atcaatcacc
      181 tgttcttcgc ggtaatgacg taaacggtta atgacataaa aagtaaccat ctcaacaaac
      241 tcttccagcc ctgtttcgcg gaaatttaat gaagaaatca ctgtttccac ataggtgaga
      301 aaagcgttat tggtccggta tacctgcgaa gcaacaaagc aataaggcaa caacggcagg
      361 taatgctgct caaaaaagcg tttactgatc ccaacgttca atatgcgcgt ggcaccaaac
      421 tcataaaaac tttgatggtg cgaacctaac ggaataaaaa caaaatcgcc ccgttccagt
      481 aacacgcgct taccgttaat ctcctggaaa taacgcccgg ttaataccag agtaaattca
      541 taatagtcgt gctgatgcag tccgctgata ctctcagttt tgttatagat aaacacatgg
      601 aagtttttgc cattaaacaa ctgctgttct cgggcagtgg caatttccgg cgcgttaatc
      661 actggctgca tcatcgactc cttatgcctt cagtttttca tgaagctcaa ttaattcagt
      721 aatcagttca cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga ccagcaccag
      781 actcactttc atcttacctt cgcccgcatc gccttcaatc agtttcgtct gtaccagatg
      841 cgcttcattc aatgccatcc gtgactgatc catcatggct ttcgctgcgg caaaatcgcc
      901 ctgcttcgcc tgtttcaatg cggcatacgc caggctacgc gcttgtccag agttgatgat
      961 aagccccatc accacttctt ccagctcttc agcttctgtt tgcgtatcag gaatattatc
     1021 gagatccatc atacatcgtt cctctctctt cttaccggca agattacccg taccggcatc
     1081 gattaaaatt tcaggggtta gcgatatctt cttcgctctc ttctttatca atcgcatttt
     1141 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgatg ccaaggttga
     1201 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     1261 ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg
     1321 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     1381 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
     1441 gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga
     1501 tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg
     1561 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     1621 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     1681 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     1741 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     1801 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     1861 agccgggaat taatgccgag aaagaacgcg atactgaagc aggtacgctg tcgggcagtt
     1921 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     1981 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
     2041 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca gccgccagcg
     2101 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     2161 ttattcctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
     2221 caatggttga ggcgtcgagg cgaataccta aggaataaaa aacgacccct ccccaaagct
     2281 tagaaaaacg ttgttaatta atacaaacat ggcccccgca agggttaacg gcattaagcg
     2341 aataaagcca tttttgattg cattaacgtg tggctgcttt cctattttaa ctgcaaaagg
     2401 gaggagtacc ttttcaagcg atgcaataac attactcata gaaaaatacc cttaaaaacc
     2461 gcaatttaaa tattacggta ttgatttatg aaataactct ttgacgggaa aatttaaaat
     2521 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
     2581 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
     2641 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
     2701 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
     2761 ttctgcctgt gcgcgcattt ttgataccag taaagagata gacatgcccg cagaacaaaa
     2821 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
     2881 cactccgcaa gccttgacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
     2941 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
     3001 taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg
     3061 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
     3121 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
     3181 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
     3241 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
     3301 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
     3361 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagctgtac
     3421 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
     3481 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
     3541 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     3601 attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag gaagttttgt
     3661 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     3721 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     3781 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     3841 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg aaactcgcaa
     3901 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg acgaacaaga
     3961 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     4021 cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga gcgattttgt
     4081 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     4141 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     4201 atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac gccagggtaa
     4261 acagttactg acggcattag gttgcccgga acacctttat aagaaagccc caactgccga
     4321 tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     4381 tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa caatagagaa
     4441 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg atgatttctg
     4501 gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     4561 atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac attgcaggag
     4621 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     4681 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     4741 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     4801 ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     4861 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     4921 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     4981 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     5041 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     5101 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     5161 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     5221 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     5281 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     5341 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt tggccttggc
     5401 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     5461 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     5521 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     5581 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     5641 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacacgcc
     5701 ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca caaacaccac
     5761 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     5821 gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg tcagtttcgc
     5881 ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     5941 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     6001 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     6061 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg
     6121 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     6181 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     6241 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     6301 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     6361 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     6421 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     6481 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     6541 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     6601 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgtggatct
     6661 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     6721 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     6781 cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     6841 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     6901 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     6961 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     7021 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaactatacc
     7081 ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat ttataagaaa
     7141 gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt tttcgagcat
     7201 taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat actctacatc
     7261 atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg
     7321 cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt ggcgagtaat
     7381 ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac tctcaccaga
     7441 tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa
     7501 gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc tgacgctgaa
     7561 atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc ccgccgcacc
     7621 cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg
     7681 cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca cagattcttt
     7741 gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac aggtttctcc
     7801 gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc tatggcaata
     7861 acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc gccaacgtaa
     7921 cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca caggtgccgt
     7981 ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg ttaatggcgt
     8041 cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt
     8101 gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg ccccctccct
     8161 cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca tccagtgctt
     8221 cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc agcagtaaga cgatcgcggt
     8281 agtaacgatc cacccagtca ttgagcgcat taaacaaggt atcgttcatc atcaccgccg
     8341 gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg cacgccgggc
     8401 cgccgccatt cgccatgctt tcacgtaagt caaagacttt tagttcgcta atcgggttgt
     8461 cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga cactcctgag
     8521 gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta aacagatagg
     8581 tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc ataaaaccgt
     8641 tcacccgcgt gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac
     8701 ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg ctctggtcga taacgtccgg
     8761 gttttgctgg gcgaaaatca cctgttgggg attcacctga ttaagccttg ccaccgcctc
     8821 gctggcttcg cgagtctgtc gcgccggata acgggaaggc cgggtatcat tgccttcttc
     8881 tcgcccgtag acaaaaagta gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg
     8941 gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc aacgccgaat ggacgctaaa
     9001 tttctcttcg tccttaaaaa tcgcttttaa cagcgattca gtgacgggcg cttccagcga
     9061 acggtgaaat ttattgttca ggttggcaac ggtgagatgc actttgccat ccagcgtatc
     9121 ggcagatggc gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct
     9181 ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact
     9241 gaatcccaac tgacgcagca ccggaataaa cggacgctcg tgcggcggaa tcacggcctg
     9301 ggggaatccc gcatcggcaa gggctttcat tttcagtaag ccctgttttg ccgccagtcg
     9361 cgggttagac acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggcccgc
     9421 gtaatgatgc gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc
     9481 acctcatcgg aaaaatccag cccggggtta agcgtggcgg gcaatgttaa cgagtccgac
     9541 tccaggctcg ccataggcca tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg
     9601 ttaccggaag caccaatgcc gccgaatggg gcggtactgg cagcaccagt aagcggtttg
     9661 ttccagttaa caatccccgc ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg
     9721 ggggaaacca gaccgcaaaa gagtccgaag cgagtgttgt tcgccatccg aatcgcctca
     9781 tcaaaagtat cataacgcca gacgcgcaaa aacggcccga acacctcttc atccggcaca
     9841 ccgccaacgc ccgtcatttc aatgatccct ggtgtcagca acgatgtccc tgcttgtaat
     9901 aagcgcggcg caagcagggt tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc
     9961 acctgctgtg cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc
    10021 cagttgcccg gcgttaatcg ctggctgacg gcaaccagac tagcaagaaa cgcatcgccc
    10081 tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca
    10141 aacgccgact gaatggtcag atggacagcc gcgtcgatat ccgccacctc atcgataatt
    10201 agcggattat taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg
    10261 cgatgcagct ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc
    10321 agcgcactca gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc
    10381 ggcaagccag cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg
    10441 ctgggtttaa agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc
    10501 aaatgaccag ggaaattata cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc
    10561 aggctcgccg caccgtccgg catttcacta cgctgctcgc cggtacgaac gtgatacgcc
    10621 ttaattgata tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc
    10681 ggcttacccg tttctctggc aataatcgcg gttaattcgg ctttattgct ttccagcaat
    10741 ccggcaaagc gttcgacaac gacctgacgt tcagccaatg agagccgcgc ccagcgcgga
    10801 aacgccgcac gggctgcccg acaagcctgt tcgacctgag cggcatcggc atcattgcct
    10861 tgccataaca cctcgcccga taccggatta cgcttcacac gcgatgcgcc ctggcccgtt
    10921 atccagtcac cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca
    10981 gacgaacgcg atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca
    11041 aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga taattttcat
    11101 tggcgaccag gcaggccgga aaatcgccct gcgcaggctg cccttctgcc acttccacca
    11161 gccgactttt acggatggcg cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt
    11221 cgaagatgtc gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag
    11281 gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg
    11341 tatagatcgg atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga
    11401 gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg
    11461 gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc aaccactttg tcattaaact
    11521 tgtcgcgaaa agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct
    11581 ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt
    11641 tactaagaaa cagcgtcggc aatgcgttat agacattcag ctcttttgag gcgtgaacca
    11701 acgtgccgac gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatagcgc
    11761 aaatccccgc cacggtgcct gtctcgctat cttccagcac gaacacatag ccctgctcgc
    11821 ttttgggcag ttcaccttgc catgttttga ttgccctttc gatacgcgcc gaaagcgtgg
    11881 cttcattggc gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg
    11941 ccgagacatc tgagcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa
    12001 gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt
    12061 gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt
    12121 cgccgcttcc agagagatct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc
    12181 aatcaacaaa cctaagccgc gaacttcact gaacaaacca tagcggtgat taatagtatt
    12241 aagacgctca acaaaccagt cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt
    12301 gatgagctcc agcactttgc ctgctaccgc cgaggccagc ggattaccgc cataggtggt
    12361 gccatgagtg ccaacggtca tcacgcgggc gcactcttcg gttgccaaca acgcaccgac
    12421 cgggaaaccg ccgcccagcg ctttggcggt ggttaacaga tcgggcgtca cgccgtagtg
    12481 catataggca tacagctcac cagtgcgccc gacgccggtt tgtacctcat caaaaatcag
    12541 caaggcattg tggcggtcac acaattcacg cagaccttgt aaaaacgcgt tgctggctgg
    12601 caccacaccg ccttcgccct agatgggctc gacaatcacc gcacaggtgg cgtcgtcaat
    12661 cagcgcgctg gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg
    12721 cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc
    12781 atgaaacgca tttttgaacg ccacgatgcc actcttatgg ctgccgtagc ggtcgtgagc
    12841 gaatttacgc gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa
    12901 gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt
    12961 ggtgtaaccg ttgccggtat gccagaactt actcgcctgt tcgttcagcg cttcacgcag
    13021 ttccggatgc gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc
    13081 tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt accggtataa agggtgccgg
    13141 agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc
    13201 gacctctaca gtaaataatt cgttatttgt atgttaacaa taagtaatgt ttgcgctgta
    13261 aatgtagatt gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa
    13321 ctggaaatca aggagttata accaaaccat atgcatttaa agtgcatata aagtgaatac
    13381 gtttgcgatg tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat
    13441 aattctagaa ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca
    13501 gcgcgcacca aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc
    13561 aaatcactta acaacaggcg gtaagcaacg cgaaattctg ctaccatcca cgcactcttt
    13621 atctgaataa atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc
    13681 cagacctcac cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca
    13741 ggagacaaaa gttcatgacg atatgtttcc gctcgaagag gtggcgaagc tcggctacaa
    13801 cgtgttttat cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc
    13861 gattgccgtg cgtcgcggct ttcccggtga cgacgaagag gcgcagcggc ggattattat
    13921 ggcggaaatc ccctcactgc tgggtaatgt caccgtgatc aacggttact tcccgcaggg
    13981 tgaaagccgc gaccatccga taaaattccc ggcaaaagcg cagttttatc agaatctgca
    14041 aaactacctg gaaaccgaac tcaaacgtga taatccggta ctgattatgg gcgatatgaa
    14101 tatcagccct acagatctgg atatcggcat tggcgaagaa aaccgtaagc gctggctgcg
    14161 taccggtaaa tgctctttcc tgccggaaga gcgcgaatgg atggacaggc tgatgagctg
    14221 gggggtggtc gataccttcc gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt
    14281 tgattaccgc tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc
    14341 cagccaaccg ctggcagaat gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat
    14401 ggaaaaaccg tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagcagctc
    14461 tcctggctca aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa
    14521 tgatttgttg cctgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact
    14581 tctctattat caagtttgat ataggaaact ccacgatgaa cgctgagcgt aactttctgt
    14641 ttgcctgtct tatttttgcg ctggtcattt acgctatcca cgctttcggt ttattcgatc
    14701 tgctcaccga tttaccccac ttacagacac tcattcgcca gagcggattg ttcggttata
    14761 gcctctatat tctgttattc atcaatgccc ccctctttct gttaccagga agcatactgg
    14821 tgatcgctgg aggaatagtt tttggcccgc tcttagggac acaactctca ttaattgccg
    14881 caacgctggc ctcctcgtgc tcattcctgc tggcgcgctg gctgggacgt gatttactgc
    14941 tgaaatacgt gggccatagc cataccttcc aggccattga aaaaggcatt gcgcgtaacg
    15001 gtattgattt tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt
    15061 acgcttacgg attcaccacc atcgccttct ggccttatac ccttatttcg gcactcacga
    15121 ccctgcccgg tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta
    15181 cgctgcgctt tattttacaa ctctgtctgg cgggcctggc gctgtttatt ctcatccagc
    15241 tcgcaaaact ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac
    15301 ttactcaccc aaaaaatgaa ggatagaacg atgtcgcaac attattcagt gtcatggaaa
    15361 aaaggactgg ctgcactctg cttactggct gttgccgggc ttagcggctg cgatcaaaaa
    15421 gagaatgcgg cggcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgcgtc
    15481 gatgccaata accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac
    15541 gacactcgtc acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg
    15601 gcttatgcgc ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacgccgggc
    15661 gaaaacatga cgatggataa taaagaaacg actcatgtca caggcagcaa actggatatt
    15721 tcggtcaact ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat tgttgacagt
    15781 aatggcaaga aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa
    15841 acgggttgcc tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac
    15901 acttatggtg cggtggaaaa acacggcgaa gtgaaattca aaggcaatgc ctcagttctc
    15961 ccggcggata acacgctggc aacggttacc tttaaaatca ccgaataaag ccaagataaa
    16021 ggatgatgaa gatacaatcg cgtaaaatct ggtactaccg tataaccctc atcatcctgt
    16081 tgttcgctat gctgctcgca tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca
    16141 gcgttgcggc gtttgccgcc gtggaccaac agggtataga acgctttatt cagtcttacg
    16201 gcgcactggc ggcagttgtc tcgttcttgt tgatgatttt acaggccatt gctgcaccgc
    16261 tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct
    16321 tgctgtcgtg gaccagttcg atggccggcg cggcgctgtg cttttttatc gccagagtga
    16381 tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt gcttgacagt atggacggct
    16441 ttttcactcg ctacggcaaa cacaccatcc tggtctgtcg gttattgcct tttgtccctt
    16501 tcgatccaat cagctatgct gccggtctga cttcaatacg ttttcgctcg ttttttatcg
    16561 ccaccgggct tggtcaatta ccggcgacta ttgtttattc ctgggcaggc agcatgttaa
    16621 caggcggtac tttctggttt gtcaccgaac tgtttattct gtttgccctg accgtggtga
    16681 tttttatggc gaagaaaata tggcttgagc gccagaagag gaatgactga tgggtttacc
    16741 gccgcttagc aaaattcctt tcattttacg tccgcaggcg tggctgcatc gtcgccatta
    16801 cggcgaggtg ctaagcccga ttcgctggtg ggggcggatc ccgtttatct tttatttggt
    16861 gtcaatgttt gttgggtggc tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc
    16921 gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag
    16981 tatgaaagtc gccgagcgta ccggcagcac cgataaactg ctggcagtgg ctgactggcg
    17041 gcaaagcccg ctctttagcg atgaagaacg gctggcgctg gagtacgccg aagccgcaag
    17101 cgtcacaccg ccaacagtcg atgatgccct gcgtacccga ctggctgcgc attttgacgc
    17161 tcaggcgctc accgaactga cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa
    17221 ttctgccatg gacattcccg ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg
    17281 agggaagatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctttggcaac
    17341 acatgccagt gactggcagg aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa
    17401 cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg gcgagatgaa
    17461 aacccattac gctataaacc tgaagattgt tcgtctcgcg gatgccgcag acgcggtgaa
    17521 gcgcattcag accgaagctg ctgccggtcg taaaacgggc ggctcggtgg atctgctctg
    17581 ggtgaacggc gaaaacttcc gcaccttaaa agagtaatgc cagtcagtta agcaactgac
    17641 tggcatt
//
LOCUS       DAJTMX010000085        17385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTMX010000085 DAJTMX010000000
VERSION     DAJTMX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.085"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="OO145_002761"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="OO145_002761"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5467824.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="OO145_002762"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="OO145_002762"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5467825.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="OO145_002763"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="OO145_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5467826.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="OO145_002764"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="OO145_002764"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5467827.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="OO145_002765"
     CDS             complement(5464..5661)
                     /locus_tag="OO145_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5467828.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="OO145_002766"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="OO145_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5467829.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="OO145_002767"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="OO145_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5467830.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="OO145_002768"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="OO145_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5467831.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="OO145_002769"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="OO145_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5467832.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="OO145_002770"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="OO145_002770"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5467833.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="OO145_002771"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="OO145_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5467834.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="OO145_002772"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="OO145_002772"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5467835.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="OO145_002773"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="OO145_002773"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5467836.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="OO145_002774"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="OO145_002774"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5467837.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="OO145_002775"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="OO145_002775"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5467838.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="OO145_002776"
     CDS             complement(17102..17155)
                     /locus_tag="OO145_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467839.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3942 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DAJTMX010000086        17265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTMX010000086 DAJTMX010000000
VERSION     DAJTMX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.086"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO145_002777"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO145_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5467840.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO145_002778"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO145_002778"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5467841.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO145_002779"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO145_002779"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5467842.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO145_002780"
     CDS             complement(2205..2348)
                     /locus_tag="OO145_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5467843.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO145_002781"
     CDS             complement(3019..3306)
                     /locus_tag="OO145_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5467844.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO145_002782"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO145_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5467845.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO145_002783"
     CDS             3683..3922
                     /locus_tag="OO145_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467846.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO145_002784"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO145_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5467847.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO145_002785"
     CDS             5033..6406
                     /locus_tag="OO145_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5467848.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO145_002786"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO145_002786"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5467849.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO145_002787"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO145_002787"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5467850.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO145_002788"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO145_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5467851.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO145_002789"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO145_002789"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5467852.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO145_002790"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO145_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5467853.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO145_002791"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO145_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO145_002792"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO145_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO145_002793"
     CDS             16524..16760
                     /locus_tag="OO145_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5467854.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OO145_002794"
     CDS             16865..17056
                     /locus_tag="OO145_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5467855.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17265)
                     /locus_tag="OO145_002795"
     CDS             complement(17081..>17265)
                     /locus_tag="OO145_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5467856.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4187 a         4354 c         4161 g         4563 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DAJTMX010000087        17090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTMX010000087 DAJTMX010000000
VERSION     DAJTMX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.087"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..507
                     /locus_tag="OO145_002796"
     CDS             <1..507
                     /locus_tag="OO145_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5467857.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(508..1668)
                     /gene="ybdL"
                     /locus_tag="OO145_002797"
     CDS             complement(508..1668)
                     /gene="ybdL"
                     /locus_tag="OO145_002797"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5467858.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1777..2865
                     /gene="hcxA"
                     /locus_tag="OO145_002798"
     CDS             1777..2865
                     /gene="hcxA"
                     /locus_tag="OO145_002798"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5467859.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2875..3072)
                     /locus_tag="OO145_002799"
     CDS             complement(2875..3072)
                     /locus_tag="OO145_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5467860.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3254..5359)
                     /gene="cstA"
                     /locus_tag="OO145_002800"
     CDS             complement(3254..5359)
                     /gene="cstA"
                     /locus_tag="OO145_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5467861.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5540..5953)
                     /gene="entH"
                     /locus_tag="OO145_002801"
     CDS             complement(5540..5953)
                     /gene="entH"
                     /locus_tag="OO145_002801"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5467862.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5956..6702)
                     /gene="entA"
                     /locus_tag="OO145_002802"
     CDS             complement(5956..6702)
                     /gene="entA"
                     /locus_tag="OO145_002802"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5467863.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6702..7559)
                     /gene="entB"
                     /locus_tag="OO145_002803"
     CDS             complement(6702..7559)
                     /gene="entB"
                     /locus_tag="OO145_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5467864.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7573..9183)
                     /gene="entE"
                     /locus_tag="OO145_002804"
     CDS             complement(7573..9183)
                     /gene="entE"
                     /locus_tag="OO145_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5467865.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9193..10368)
                     /gene="entC"
                     /locus_tag="OO145_002805"
                     /pseudo
     CDS             complement(9193..10368)
                     /gene="entC"
                     /locus_tag="OO145_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10660..11616
                     /gene="fepB"
                     /locus_tag="OO145_002806"
     CDS             10660..11616
                     /gene="fepB"
                     /locus_tag="OO145_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5467866.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11620..12852)
                     /gene="entS"
                     /locus_tag="OO145_002807"
     CDS             complement(11620..12852)
                     /gene="entS"
                     /locus_tag="OO145_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5467867.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12963..13967
                     /gene="fepD"
                     /locus_tag="OO145_002808"
     CDS             12963..13967
                     /gene="fepD"
                     /locus_tag="OO145_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5467868.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13964..14956
                     /gene="fepG"
                     /locus_tag="OO145_002809"
     CDS             13964..14956
                     /gene="fepG"
                     /locus_tag="OO145_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5467869.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14953..15768
                     /gene="fepC"
                     /locus_tag="OO145_002810"
     CDS             14953..15768
                     /gene="fepC"
                     /locus_tag="OO145_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5467870.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15765..16898)
                     /gene="fepE"
                     /locus_tag="OO145_002811"
                     /pseudo
     CDS             complement(15765..16898)
                     /gene="fepE"
                     /locus_tag="OO145_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3992 a         4862 c         4445 g         3791 t
ORIGIN      
        1 tttcgtgaaa agccagtgga ttgcgttctg tgggttaaaa acagccaact tatgcctaat
       61 gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac tgctgcaaaa atccattgaa
      121 atagatggtt ttacccaacc cattgtggtc acacatacgg ataaaaatgc catggaaatc
      181 gtggatggtt ttcaccgcca tgaaattggt aaaggctcat catcattaaa attacgctta
      241 aaaggttatt tgccggtgac ctgtctggaa ggtacgcgta atcaacgcat cgccgcgact
      301 attcgtcata atcgcgctcg tggacgccat caaatcaccg ccatgtcgga aatcgtccgt
      361 gaacttagcc agttgggatg ggatgataat aaaattggca aagagctggg aatggacagc
      421 gacgaagtgc tgcgactgaa gcaaattaac ggcctgcaag agttgtttgc cgaccgtcaa
      481 tattcccgcg cctggacggt taaatagcta aagctggcgc aggcgttcag ctgctgccag
      541 caacgtcgat tccttcttgg caaaacagag acgaatcagt ttatgtggga agggatcggc
      601 gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc tgcgtcagcc actggcaaaa
      661 ctcaacatca tccagggtag aaaccgcgct gtaatccacc agcaaaaagt atgtaccttc
      721 acacggtaaa atctccagcc ggctttcatt taaagcattc accagaatat cgcgcttctg
      781 gcgataaaag tccggtaacg caagataatg ctcaggttct gcacgtagca tatcagcaag
      841 cgccagctgt gccggggtat tcaccgaaaa ggtcagatac tgatgtacct tgcgaatttc
      901 ggcgctgatg ggcgctggcg caacacaata acccactttc cagccggtca tatgataggt
      961 cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc tgcggatgcg ccagcacact
     1021 ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa tgacaaaaat
     1081 ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc agacagttgc
     1141 actggggtta tgcggagtgt tgaggatcac cagtctggtg cgctcgctta ataatgcggc
     1201 aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt gccatacgtt tcactattcc
     1261 cccagaaagc gcgatggcgg gggcgtaact gtcatagctg ggatcaaaac aaatcacttc
     1321 atcgccattg cgcaccagtg cggtaatcgc cgcgtataac gcttccgtcg cccctgccgt
     1381 tacggtgata tcgctatcgg catctggttg atagccatac aaacgttccg ttttctgagc
     1441 aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg tattggtttg ccccctgggc
     1501 aacgtggtac gccagccgct cctgtaaata gcgcggacca tcaaaatcag gaaagccttg
     1561 cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc atctgggtga aaatagtggt
     1621 gccaagttgt ggaagtttgc tttgtggaat cagagggtta tttgtcattt ttattgtacc
     1681 tgacgaagtt gcgtgttgaa ggcactataa cacgatgcca gtatttggca atcaagacgt
     1741 ttagatgtct aaataaaaca ataaggacaa cacaacatgc ctcacaatcc tatccgcgtg
     1801 gtcgtcggcc cggctaacta cttttcacat ccaggaagtt tcaatcacct gcacaatttt
     1861 ttcactgatg aacaactttc tcgcgcggtg tggatctacg gcaaacgcgc cattgctgcg
     1921 gcgcaaacca aacttccgcc agcgtttgga cttccagggg caaagcatat tttgtttcgt
     1981 ggtcattgca gcgaaagcga tgtacagcaa ctggcggctg agtccggtga cgatcgcagc
     2041 gtggtgattg gcgtcggcgg cggcgcactg ctcgacaccg cgaaagccct cgcccgccgt
     2101 ctcggtctgc catttgttgc cgctccgacg atcgccgcca cctgcgcagc ctggacaccg
     2161 ctctccgtct ggtataacga tgccggacag gcgctgcatt atgagatttt cgacgacgcc
     2221 aattttatgg tgctggtgga accggagatt atcctcaatg caccgcaaca atatctgctg
     2281 gcggggatcg gtgacacgct ggcgaaatgg tatgaagcgg tggtgctggc tccgcaacca
     2341 gaaacgttgc cgctaaccgt gcggctgggg atcaataatg cgcaggccat tcgcgacgtc
     2401 ttgttaaaca gtagcgaaca ggcacttgcc gatcagcaaa atcagcagtt aacgcaatca
     2461 ttttgcgatg tggtggatgc tattattgct ggtggtggga tggtcggtgg tctgggcgat
     2521 cgttttacgc gtgtggcggc ggctcatgcc gtgcataacg gtctgactgt cctgccgcaa
     2581 accgagaagt ttcttcacgg caccaaagtc gcttacggaa ttctggtgca aagcgccttg
     2641 ctgggtcagg atgatgtgct ggcgcaatta accggggcgt atcagcgttt tcatctgccg
     2701 actacgctgg cggagctgga ggtggatatc aataatcagg cggagatcga caaagtgatt
     2761 gcccacacct tgcgcccggt ggagtccatt cattacctgc cagtcacgct gacaccagat
     2821 acgttgcgtg cagcgttcga aaaagtggaa tcgtttaaag cctgacgtgc aaatttagca
     2881 gcagcgcgcg ccgccttttc caccgtagcg cgcgtcctgc cgctcacgga aaaactcctc
     2941 gtaggtcatc ggcgtttgat cgggatgatt aacccgcata tgttcgacat agttgtcgta
     3001 atcaggcata ccaatcatca gcttcgccgc ctgacctaaa tattttccgg cttttgccag
     3061 tgaatcaaac atcttggcct ccgctaacca tgttgctgaa agagtgccgg atgcggcgtg
     3121 aacgctttat ccggcctaca aatgctatca acgcactcgg tcagtcccct cgccccatcg
     3181 gggagagggt tagggtgagg gggaaccctc ggcactatgc caacattcgc caacatcccc
     3241 cctcactctg actttagtgc gcgcctttcg cctgcgctac gatctcctcg acattttccg
     3301 gcatcggctc atacggcgtt tctttcgccg ttggcttcgg atctttcaat gccgcaagtg
     3361 ccgtcttaat cgagaacagt gccaggacca ccacgaccac cataaagaag atggttaacc
     3421 cggcatccag acggttgttg aacaccagtt gcgccagctg tgactcagta tactgcgacg
     3481 gaatattgcc gctgtcgatc attgcctgga acttgttagc aatgaccagg aagccgactt
     3541 tcgcatccgg gctaaacgct ttcagccagt cagcggtcag ggtacaaatc agcagccagg
     3601 ccgttggtac cagcgccacc caggcgtaac gttgacgttt catcttgaac aacaccacgg
     3661 cacagagcat cagcgccatc cctgccagca tctggttggc aataccaaac agcggccaca
     3721 gagtgttaat accgcccagc ggatcgacca cgctctgatg gaggaagtag ccccacgcca
     3781 gtacgcacag cgctgttgcc agcaggttag caggcagtga atcggtccgc ttcaggccag
     3841 gagacaccac gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta cccgcatcta
     3901 ccgccgtcag aataaacaga gcttcaaaca gaatggcgaa gtgataccag aacgccacat
     3961 ccatcatacc acccagcgca ccgtgcagga tgtaagccat ccccaccgcc aacgtcggcg
     4021 caccgcccgc acgggaaatg atcgactgtt cacccacttc gctggcaatc tggttgagcg
     4081 tatctggtgt aatgctaaag ccccagctac tcaccacctg cgcggcagaa gcgactacat
     4141 ccgccgtccc tgccggagcc agcaccgcca ttgggctgtt catggcaaaa tacacgcccg
     4201 gatcgatgat acaggcagaa actagcgcca taatcgccac gaaggattcc atcaacatcc
     4261 caccgtagcc gataaagcac gcctgccctt cgttcgccag catcttcggc gtggtgccgg
     4321 aagagatcag cgcatggaag ccagacaccg caccacaggc gatggtgata aacaggaacg
     4381 ggaacaggtt accggtccat accgggccag tgccatcgac aaatttggtc agcgcaggca
     4441 tggtcagcgt cgggcgcata atcaaaatgc ctaccgccag accgacgata gtcccgattt
     4501 tcaggaaggt cgagagatag tcacgcgggg ccagcagcaa ccacaccggc agcaccgccg
     4561 ccacaaaacc gtagcccacc agcatccagg tcagctgcac gccggtaaag tcaaagtacg
     4621 gtgcccaggt cggactttct gccacccagc cgccagagat aatggcgaaa atcaggaaca
     4681 ccaggccaat cactgacact tcgccaatgc ggcccggacg cagatagcgc aggtagattc
     4741 ccataaaaat cgccagcgga atggtgaacg caacggtata cgttccccac gggctatgag
     4801 tcagggcttt caccacgatc atcgccagta ctgcaaggat aatgaccatg atcataaagc
     4861 aggctaccag cgcaatcacc ccggcagttg gccccatctc ttctttgacc agctcaccca
     4921 gcgagcgacc gtcacggcgc gtagaaacaa acagcaccat gaaatcctgc accgcaccgg
     4981 cgagaaccac cccggcgagc agccagatca tccccggcag gtagcccatt tgcgctgcca
     5041 gcaccggccc caccagcggc cctgctccgg caatggccgc aaaatggtga ccgaacagca
     5101 ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg gcgcaccgct ggcgtcatac
     5161 gcgtcgggtc aaccgccagc acatttttgg cgatatacag cccataaaaa cggtaagcga
     5221 tcagatagat acagaccgac gccaccacaa tccacagcgc gttgatctgt tccccacgat
     5281 ttaaagcaat gtatcccaga gcaaatgctc ccattacaga gagcactgtc cagacgaggt
     5341 atttccctga tttgttcata gttgttatcc gtgtgcgtgt ccatagagat gttacatttt
     5401 gtttctataa catctttgta cgatttaaca acacggtaac catgtaaatc ggagttgaaa
     5461 ccaatattta acttaacaat gttaactcga tcactccgtt aaccgagttt tcctgcaatc
     5521 tcatttaatt ctgtcgggct catcccaaaa ctgccgtacc cagccgacaa gtgcagcaac
     5581 gccgcccctg ttcatcgaaa acgacgattt cccaactctg attttgccgc ccaagatgca
     5641 gcggctggca gacgccgcgt accttgcctt cagacaccgg gcgatgatgg gtggcgttaa
     5701 gttccgtgcc caccacacac tgcccgtcac gggtcatcat aaatccggcc atcgatccca
     5761 gcgtttcagc cagcgccgcc gacgcgccgc catgcagcag gccaaacggc tgatgagtac
     5821 gggtatcaac cggcatttcg gcttccagca catcatctcc cagaagggta tacacaattc
     5881 ccagatgcgc caccattgtg ttatcgctgg tggcgttcag ttcgtcgagc gttaaatggc
     5941 gtttccagat catgcttatg cccccagcgt tgagccgcca tcgaccacaa tatcctgtag
     6001 ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg
     6061 acgggcgatt ttccccagcg gaatgccgag tttaaactgc tcgccaaagc cgcgaatacg
     6121 ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc atatcggtgt cggtggagcc
     6181 aggcgaaacc acattacagc gcacgccgct acccgccagt tccagcccga cgctcaacgc
     6241 caggcttttc agcgccgctt tcgatgcgcc ataagcactc ataccaatac gcgccgtgtg
     6301 tgcggcgtcg gacgccacag tgacaatcgc cccgccccgc tgacggcgaa actggttcat
     6361 ggtttgctgg aacaggttaa acgcaccgcc gacgttaacc gcaaaagtct gctgccagtc
     6421 ctctttactg agctgatcgg tcgcgcccat gcgcaaaatt cccgccgcat tgatcagcac
     6481 gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc tgcgccacct gcccggcgtc
     6541 ggcaacatcc attacttcgg tcgcaaaggg atattgctcc tgagtgaacg cttgatcaaa
     6601 acctgtaact ttcgctcccg cctcaacaaa cgtcagcgcc gtggcgtagc cgataccttt
     6661 acctgcgccg gttacccaga catttttacc gctgaaatcc attatttcac ctcgcgggag
     6721 agtagcttcc accaggcgtc gatggtcggg tttttcgcca gcatgacaaa gtcgatatca
     6781 ccatgcactt tgcgccagcg cgccgccagc gccatcatgc gcaccgaatc cagaccgtag
     6841 tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg caggatcacc
     6901 tcacgcagcg ccgctttgct ggcggggata ggtgctggca gtaattcttc ggtcatcacc
     6961 acccggccag aacgtccggc cacatatttc agcgacatca aatgctcgtc acggctgaaa
     7021 tcagccagcg catccgccac cataaacggt ttaatatcgc gcataaatgc gtcggttgcg
     7081 gtggtcatac agccaatgtg ggcatatact cctgtaataa tcagctggtt acgtccactc
     7141 tctttgagca tttgctccag cggagaacga tgaaacgcgc tgtagcgcca cttcaccagc
     7201 accgtgtcgt cggcatctgg cgtcaggcga tccaccacct tttgctgttc cggcgagcgg
     7261 gtcaggcccg gcccccacat atcattcaac agcgcccgat cttcatcgct ctgctctttc
     7321 ggctgggcgg tgtaataaac cgggatattg tgctgtttgc agtagtcgcg cagcgcagca
     7381 atattcgcga tcacctgctc catcatcggg cagttctcgc cccagaagct gacaaaatag
     7441 tcctgcatat catggattaa caacgcggca cgttgcggtt caaaggccca gtcaacttta
     7501 ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg aatagccatc
     7561 gtgttctctc cttcaggctg atgcgcgtga cgccagccac tgacgtaatt gttttttatc
     7621 gactttcccg accgccgtca gcggaagtga atccacacac tccacgcgat ccggtaattt
     7681 aaattcggca ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc gcagcggctc
     7741 ttttaccacc agataagcac agcttttttc gcccatcagc tcattttcca tgctcaccag
     7801 tgcggcatag atcactgccg gatggctcag cagcaggttt tcgatatctt cggcagcgat
     7861 cttctcgccg ccacggttga tctgatcttt ctcgcgcccc tgcacggtga tgtaaccctc
     7921 tggatcaata gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa aggcactggc
     7981 attgtgctgc ggacttttgt aatagccacg gaaggtgtac ggcccgcgcg tcatcaggcg
     8041 tccaacttcc ccttgcggca gtggatttcc ttcggcatcg gcaacccaca cttcatcgtc
     8101 cggacacatt gggtaaccct gggtatggat aattttctcc gcgctatcat caagacgggt
     8161 gtagttcacc agcccttccg ccatgccaaa cacctgctgc aactggcagc caatctcagc
     8221 gggaatacgc gctgcaagcg tggcagaaag acgtgcgccg ccgacctgta acagtttcag
     8281 cgaggcaagc tgcgcccggc tttcgccttc agccagcgcc tgcaaccaca ggctgactgc
     8341 cggcggcacc agcgcggtaa cgttaacctg atgttttgca atcaacggga agcaaagcgt
     8401 ggcgctggga tcggccgcca gaacaaccgt tcctccggca agaaagacgc ccagcgatcc
     8461 tggcgaactc atagtgtagt tatgagccgc cgggatcgag cacaggtagc gcgtctgttg
     8521 tgtgaactga caaatctcgg cgctacgacg cacgctgtag taatagtcgt tatgagtgcg
     8581 cgggatcagt ttcggtgtgc cggtggtgcc gccggaaagc tggaaatagg ccacttcatc
     8641 agcaggtgat ggcgtggcag taaaaccctc tgccggatgg ttaatcgcat cctgcaagtt
     8701 atgctcaccg ctgtcgttgt gcagttgcac cacgcgaatg gaagaatgct ctgcaacaaa
     8761 tgtgttgagg aaatcatccc cgctaaatag cgcatgctgg cgatcggcaa tcagtaacgc
     8821 cggttcaatc tggctggcgt aggcgttcag ttcactacgc tgatggctga acagcgccag
     8881 caccggcgcg acgcccagtt tcagcagcgc gaaaaaggta atgtaaagtt cagcgacgtt
     8941 acccagttgt accagcgcgg tttcaccagg tttaatgccc tgacggcgca aactacaggc
     9001 gaggttatcc gccgcctgat tcagctcccg ataactcaac tgccgctcgc cgtcgataac
     9061 cgcgatgctg tcactcgcag cgtggcgagt cagaatgtcg gtcagtggca aatcctgcca
     9121 gtagcctttt tcccgatagc gacgggcaaa ctcttccggc cagcgggtga atggaatgct
     9181 catcctcgct ccttaatgca atccaaaaac gttcaacatg gtagaaagtt tgacgcctgt
     9241 ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc cccgctccgg caaacagacg
     9301 cacctgattt tcccgcagct tcgcgcagcg gatggtcacc acccattcgc cgttaccttc
     9361 gctgtcacac caacccacaa tgccgccaaa cagttcgcgg tcgaacggct ccagttcagc
     9421 aataacctgg gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg gatgcagcag
     9481 acaggccaga gtcagtgcgt tttcttgcga attcgcttta ccttcaaagg gagttgcgag
     9541 atgccacagc gtcggcgtgg taatcaattg tggagaggag ggaacgtgta actcactact
     9601 gcgttcgcgc agtacctctt tcatcgcctg agtccccagt tcatgttcat ggcgatcttt
     9661 ttctgacgcc agcagacgat tacccgcttc gcgatcgagc acttcatccg gctgacgacg
     9721 cgcggaaccg gctaacggaa tggagctaaa acgctcgccg tctttacgta gcagcagttc
     9781 cgggctggcc cccagcagga cgccaccatc agccagcggg acatggaagt tgtaactaac
     9841 cgggttttgc gcaatcaacc gttccagcaa tacgccacta tcaatggcgg cgtcagtggt
     9901 gatatcaatc aatcgtgaca acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc
     9961 ggcgcgggca cccatctgtt caaacgtggt ttgttcagga attgcctggc gttccaccac
    10021 gttcagcgac tggctgcggg taaaacagcg ggctgaggtc tgtttttcct gacgggagaa
    10081 cgactgctag gattcgggaa tatacagcga cgaaggctga cgtggatcga agggaatcgc
    10141 cccgaccatc accggatttt tgatgccctg cgctttggca tcggcaaaca gcgcggcgag
    10201 tttttgctgg aagggactgt cgggcgaatc cccgttcaca gccggttcat cgaaacgggc
    10261 gaaacatcct gacgtcgtaa aactgcggta cggcgacata aagaaaaagc gattgggcgc
    10321 aagtgttgcc atggtctgct gtacttcctc agccagtgac gtatccatat catcctccac
    10381 aaaatgataa aggctttaat aatgattatc acttatattt tcggccgcta acctaaaagc
    10441 aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc aactcggggt tgcatctggt
    10501 tgtggttaca atgaaaatga gaagcattat tgatgaattc gcataagcgc aatgtgatgt
    10561 cctgcgccgt tttgccccct ctccccaaag ggacggattg tgcgctttgt cgaatttgtc
    10621 attacgccct taaccttatt aataacagga agctgacttg tgagactcgc cccgctctac
    10681 cgcaacgccc ttctattaac aggacttttg ctttcaggaa tagccgtagt tcaggccgcc
    10741 gactggccgc gtcagattac tgacagccgg ggcactcata ccctggaaag ccagccgcag
    10801 cgtattgttt ccaccagcgt caccctgacc ggatcactgc tggcgattga tgctccggtg
    10861 atcgccagcg gcgcgaccac gccgaataac cgcgtcgcag atgaccaggg ctttttacgc
    10921 cagtggagca aggtggcgaa agaacgcaaa ctgcaacggc tctatatcgg cgaaccgagc
    10981 gccgaagccg ttgccgcgca aatgccggat ctgattttaa ttagcgcaac cggcggggat
    11041 tcggcgctgg cgctgtatga tcagctttcc accatcgccc cgacattaat catcaattac
    11101 gacgacaaaa gctggcagtc gctgttaacg cagcttggcg aaattaccgg gcatgagaaa
    11161 caagcggcag agcggattgc acagtttgat aagcaactgg cagcggcgaa agagcaaatc
    11221 aaattaccgc cgcagccggt cactgccatt gtctataccg ccgctgcaca cagtgccaat
    11281 ctctggacgc cggaatcagc acaagggcag atgctggaac aactcggctt tacgctggcg
    11341 aaattgcccg cagggttaaa cgccagccaa agccagggca aacgccatga catcattcag
    11401 cttggtgggg aaaatctggc tgcggggtta aacggtgaat cgctattcct gttcgctggt
    11461 gatcagaaag atgccgatgc gatttatgcc aatccactgc tcgcgcacct gcctgcggta
    11521 caaaacaagc aggtttatgc gctgggaacc gagacgttcc gcctggatta ctacagcgcc
    11581 atgcaagtgc tggaacggct taaggcgctg ttttaagcat taactgtcgg acgctgtcac
    11641 ctgcggcggc gtctggcgaa aacgtcgcaa ctccaccagc accagcaata acaacacgcc
    11701 gataatcaac aaaccaaaac cgctcgcgct tgcggaagca accggtgtca tcatcgcgcc
    11761 caaaccaccc agcagcgccg cgcctatggc atcgcccgtc acgttctgcg ccgtccacaa
    11821 accgttaatc cgccctaaca tcgcttccgg ggtttgcgtt tgcagcattg tatattgcag
    11881 caacgaacta accgcgctca gccagccgaa cagcgccaga caaaccacgc ctaaaatcca
    11941 catcggcatc aggccaaaca gaccaatggc gaggaacgat cccagcgtgg agagcagcat
    12001 caataaccct ggtcgcgcac tatgtgccag cttcccgctg gttaacgcac caatagccgc
    12061 gccgagcggg atcgccgcgt agagaaaacc aatctgtgcc gctgacatct gccagttgtc
    12121 agccagcgcc ggatacagta ccagcaccgc gctcgccatc gtcaataaac cacccagcag
    12181 cgggctggca agcagaaaac gaaatcctgc cagtaatgat ttcaacggat gctcaagcgg
    12241 ctgcggtggc ggtggcaact ccggaaggct taataacggt aacaaggtaa taaacgtgcc
    12301 cgccgccgcc agcccgtagt tccaggctac gccgccggtc gccagcaata aaccgccaat
    12361 catgggcgaa atcaccgacc ccaggcgcac ggtcaacatg gtgatcgccc cggcctgcat
    12421 taagttttca cgccctacca gtgcagatgt tgccgccagt agcgccgtaa caccaagtga
    12481 tgcgaaaaaa ccatcccata aaccaagtaa atagattgcc agcaatgacg gctccggcag
    12541 cagtgcatta aggcacagtc caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat
    12601 cacttttttg cgttcatagc gatccgccag cacaccgcca accatcaggc caacaaacat
    12661 cgcgccgccg gtcagcgtca ccgaaagccc cacctgccag gtagaatggg tcatcatctg
    12721 gatctgcacc ggcaccgcga cgccgagcaa acccagagac acaattgaga taaaacgagc
    12781 gaggaatact gcgcgaaacg ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga
    12841 ttgtttattc attacaatgc cttgccatca aatattttaa atacctattc ttaggctgca
    12901 catgttaaca tatccaaata agatcgataa cgataattaa tttcattatc atggaagttc
    12961 gtatatctgg ttctgttgcc gtgacacgcg ccattgccgt gcccggattg ctgttattac
    13021 tgattatcgc gacggcatta agcctgctca ttggggcaaa atcactcccc gcttccgtag
    13081 tgctggaggc cttctccggc acctgccaga gcgccgactg caccatcgtg ctcgacgcgc
    13141 ggctgccgcg tacccttgcc ggtttactgg caggcggcgc gcttggcctt gccggggcgt
    13201 taatgcaaac cctcacccga aacccacttg ccgaccccgg cttgcttggc gtgaacgccg
    13261 gagccagctt tgccattgtg ctgggtgcgg cgctgtttgg ttactcatcc gcacaggaac
    13321 aactggcgat ggccttcgcc ggggcgctgg tggcctcatt gattgttgcc tttaccggca
    13381 gtcagggtgg agggcagtta agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg
    13441 cggtgctgga aggactgacc agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac
    13501 tgcgtttctg gcaagccggt tcgctggata ttcgcaatct acatacctta aaagtggtgc
    13561 tgatcccggt tctgatcgcc ggagcaactg cgctattact gagtcgcgcg ctgaacagtt
    13621 tgagcctcgg cagtgacacc gcgacggcgc tgggcagtcg cgtggcgcgc acacagttga
    13681 ttggtctgct ggcgattacc gtgctttgtg gtagtgcgac ggcggtagtt ggcccgattg
    13741 cctttatcgg cctgatgatg ccgcacatgg cgcgctggct ggtaggtgcc gatcatcgct
    13801 ggtcgctgcc cgtcacgcta cttgctaccc ctgccctgct gctgtttgcc gatatcatcg
    13861 ggcgggtgat tgtgcccggc gaactgcgcg tttctgtggt cagtgcgttt attggtgcac
    13921 cggtgctgat cttcctcgtg cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg
    13981 ccgattactc atcacctgtt tgctgctgat ttccgcctgt gtggttgcag gtatctgggg
    14041 attacgcagc ggtgccgtca cgctggaaat ctcgcaggta ttcgccgcgt tgatgggcga
    14101 tgcgccgcgc agtatgacga tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc
    14161 gctgttgatt ggcgcggcgc tgggcgtcag cggcgcgatt tttcagtcgc tgatgcgtaa
    14221 cccgctcggc agccctgacg taatgggctt taacaccggg gcgtggagcg gcgtgctggt
    14281 ggcgatggtg ctgtttggtc aggatctgac ggctatcgcg ctggcagcaa tggtgggcgg
    14341 cattatcact tcgctgttgg tctggctgct cgcctggcgt aacggtatcg acacctttcg
    14401 gttgattatt atcggtatcg gcgttcgcgc catgctggtg gcctttaata cctggctgtt
    14461 gctgaaagcg tctttagaaa cggcgctaac agcaggtttg tggaatgccg gatcgctcaa
    14521 cggcctgacg tgggcaaaaa cctcgccttc cgcacccatc attatattga tgctcattgc
    14581 cgccaccttg ctggtgcggc gaatgcgctt gctggagatg ggcgatgata ccgcctgcgc
    14641 gctgggcgtc agcgtcgagc gttcgcgtct gttaatgatg ctggttgcag tggtgcttac
    14701 cgctgcggca acggcgcttg ccgggccgat ttcctttatt gctttagtcg cgccgcacat
    14761 tgcccgacgc attagcggca ccgctcgctg ggggctgacc caggcggcgc tgtgcggagc
    14821 gctgttactg ctggcggccg ttctctgcgc ccaacaactg tttatgccgt atcaacttcc
    14881 ggttggcgtc gttaccgtca gcctcggcgg tatttacctt atcgtcttgt taattcagga
    14941 gtctcgcaaa aaatgaccga atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga
    15001 tatggcaaat ataccgttgc ggaaaatctg actgtagaaa ttcctgatgg tcacttcacg
    15061 gcaattatcg gaccaaatgg ctgtggtaaa tccacgttac tgcgtacctt aagccgcctg
    15121 atgacgcctg ctcacgggca tgtctggctg gatggcgagc acattcaaca ttacgccagt
    15181 aaagaagttg cacgccggat tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc
    15241 accgtgcagg agctggtggc gcgtggacgt tatccgcatc aaccgctgtt tacccgctgg
    15301 cgcaaagagg atgaagaagc ggtaacgaaa gcgatgcagg ccacgggaat aactcatctg
    15361 gcagatcaaa gcgtggatac cctttctggc ggacaacgcc agcgagcgtg gatcgcgatg
    15421 gtgctggccc aggaaacggc aattatgctg ctcgacgaac ccacgacctg gctggatatc
    15481 agtcatcaga ttgatctact ggaactgtta agcgaactga accgcgagaa aggctatacc
    15541 ctggcggcgg tgctgcacga tcttaatcag gcctgtcgtt acgccagcca tttgattgca
    15601 ttgcgggaag ggaaaattgt tgctcaggga gcgccgaagg agattgtcac tgctgaactg
    15661 attgagcgca tttatggtct gcgatgcatg atcattgacg atccggtagc cggaatgccg
    15721 cttgtggtgc cgctcggacg aacggcacct tcaaccgcaa atagttaaac taagtggtct
    15781 gccatcatgg catcctgttt tctggatgcc atcgcatagc gcaataacac gctaccacaa
    15841 gccaccatcc cgccgatcag cgcggaaagg atcacaataa tcgccttacc cggaccgtct
    15901 tttttcactg gcaatgacgg gcttaactga tatttaaacg gcgtaaaatt cacatcgttg
    15961 acatttgctt ttgttaattg ctcgacaaga tactgccgat tacgtaattc accgttcagt
    16021 tccgcaacgt cagtaaccgc tttttcaatt tccagtttgc gttcaatacc gtctgcgccg
    16081 agggaaatag agaaatcggg gtcatcttta acggcctgac cattactgta tacgggcttt
    16141 ttaattcctg ccgcgttggc aatgtcgagt gaataattga ggcgctgaat gtttgcatca
    16201 agttgatttt tcgttttaat gcgatcctga gccagttttt ctttttcaaa ctgggttttg
    16261 atctccagtt tattacggac gttttctatc gactctttca ccaccaacgc agagatataa
    16321 tcgatatacc cgctcaaaac ggtctgcgcc tctttactgg ttggcgcggt aaaacttagc
    16381 gtccaggatg tatacagtga cggttcatct tttttcttac tagcattgtc atcaaccgct
    16441 ttcatttttt cgctcaatgc aacaatagcg cgatgcaaat ccagttcatc gattttcgcc
    16501 tcttttaatt agtccatcac ataaggtgat gaacgcaggt actcttccag caagctaaca
    16561 gactgaaact tcttgataaa caggttaaat gcaccgccac ggtcgatatt aacatcgaga
    16621 tcaagaacgc ggagtttggt aaaggttttc tccaggtcct gccactgaat ggcttcggcg
    16681 ggagtaataa cggctgagct ggtccatttt tgcggcagga tgaaagagat cagtaaacct
    16741 gcgcaggcaa atgcaaaaac gaccgccatg accgtttttt tggcccgcca taaaacttcg
    16801 attagattaa gtaaatcaat ttcattatta ctgggcgacg ccagaggata ctcagaaaaa
    16861 tgagcgtcac ttccctgttt aatattcaat gatgacatgg ggtataacct gaaaatgagt
    16921 ccaatagggg aaaatctcca cggcgctcac tttaccagtt ggtgagaatt atccgaagct
    16981 gaagtttgta aattccttcc actgaatgca acattcactc tatttaagag aaatattcat
    17041 caaacctata aatcaaacaa ataaaaaacc cataattatt ttctgtaatt
//
LOCUS       DAJTMX010000088        17050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTMX010000088 DAJTMX010000000
VERSION     DAJTMX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.088"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1494
                     /locus_tag="OO145_002812"
     CDS             <1..1494
                     /locus_tag="OO145_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator family protein"
                     /protein_id="HCR5467871.1"
                     /translation="MFFFCLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIH
                     CLRGTPRRWGLFGENDQEQINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHV
                     VRQGRYLSLHDEVKNFPLQHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGA
                     QTIEATSVKQLADAGVRVGDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWND
                     ARSLPLPESELVNKATALTEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDI
                     VLKTADLCSAKDDCVRLKNALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLD
                     NLVATSTAPFITHETARAAQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQ
                     WNAFQKLAQMLMHTPFNAEGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLT
                     MLGSAIYNGVQAWRRYQRHRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            1559..2227
                     /gene="yrfG"
                     /locus_tag="OO145_002813"
     CDS             1559..2227
                     /gene="yrfG"
                     /locus_tag="OO145_002813"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5467872.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            2238..2639
                     /gene="hslR"
                     /locus_tag="OO145_002814"
     CDS             2238..2639
                     /gene="hslR"
                     /locus_tag="OO145_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5467873.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            2664..3533
                     /gene="hslO"
                     /locus_tag="OO145_002815"
     CDS             2664..3533
                     /gene="hslO"
                     /locus_tag="OO145_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5467874.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRAGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(3677..5428)
                     /locus_tag="OO145_002816"
     CDS             complement(3677..5428)
                     /locus_tag="OO145_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5467875.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            5807..7429
                     /gene="pckA"
                     /locus_tag="OO145_002817"
     CDS             5807..7429
                     /gene="pckA"
                     /locus_tag="OO145_002817"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5467876.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(7505..8857)
                     /gene="envZ"
                     /locus_tag="OO145_002818"
     CDS             complement(7505..8857)
                     /gene="envZ"
                     /locus_tag="OO145_002818"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5467877.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(8854..9573)
                     /gene="ompR"
                     /locus_tag="OO145_002819"
     CDS             complement(8854..9573)
                     /gene="ompR"
                     /locus_tag="OO145_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5467878.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            9801..10277
                     /gene="greB"
                     /locus_tag="OO145_002820"
     CDS             9801..10277
                     /gene="greB"
                     /locus_tag="OO145_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5467879.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            10374..12695
                     /locus_tag="OO145_002821"
     CDS             10374..12695
                     /locus_tag="OO145_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5467880.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            13133..13360
                     /gene="feoA"
                     /locus_tag="OO145_002822"
     CDS             13133..13360
                     /gene="feoA"
                     /locus_tag="OO145_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5467881.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            13377..15698
                     /gene="feoB"
                     /locus_tag="OO145_002823"
     CDS             13377..15698
                     /gene="feoB"
                     /locus_tag="OO145_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5467882.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            15698..15934
                     /gene="feoC"
                     /locus_tag="OO145_002824"
     CDS             15698..15934
                     /gene="feoC"
                     /locus_tag="OO145_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5467883.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            16137..17024
                     /gene="rpnA"
                     /locus_tag="OO145_002825"
     CDS             16137..17024
                     /gene="rpnA"
                     /locus_tag="OO145_002825"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5467884.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT         4224 a         4551 c         4369 g         3906 t
ORIGIN      
        1 atgttctttt tctgcctgat taccccggat gtatttgttc cgtggctggc aggcggcgca
       61 ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa atcctccctg
      121 cgagaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg cgaaaacgat
      181 caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc cgcacactgg
      241 cagccataca ttgctcagga tctcggtcaa caaaccgata ttgatatcta tctcgaccgc
      301 cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta
      361 cagcactggc tgcgcagtac gattatcgct gcgggctcgc tgctagtgct gtttatgctg
      421 ttattctgga tcccgctgga tatgccgctg aaattcactc tctcatggat gaaaggcgcg
      481 cagaccattg aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg ggtaggcgat
      541 acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggaac ctggagcgcg
      601 aaaaccaatt cacctttttt accgtttgac tgctcgcaga tcatctggaa tgacgcccgc
      661 tcattaccat taccagaatc tgaactggtc aacaaagcga cggcattgac tgaagcagtt
      721 aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc attacgttca
      781 gcgattcaaa aatccggcat ggtattgctt gatgattttg gcgacattgt actgaagaca
      841 gcggatttat gttctgccaa agatgactgt gtgcgactga aaaatgcgct ggtcaatctc
      901 ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa gctcgatggc
      961 gtgaatgtgt tattacgccc ggtgagtgct gaatcgctgg ataacctggt ggcaacctcc
     1021 accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa cagtccggcc
     1081 cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca gccctggcct
     1141 tcggcatcac tttacgacta cccgccgcaa gaacagtgga acgctttcca gaaactggca
     1201 caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac
     1261 gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg cctgtggcgc
     1321 tatctcagca ccacattgct gctactgacg atgctgggta gcgccattta caatggcgta
     1381 caggcctggc gtcgttacca gcgtcatcgc actcgcatga tggagattca ggcctattat
     1441 gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga ataacacgtt
     1501 tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga tttccctcat
     1561 gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg acggcacgtt
     1621 gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa catggggcgc
     1681 gaaaaacggg gttacgccac aggaagcgat ggaatatatg cgccagcaat atcacgacgt
     1741 acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg gtctggatat
     1801 ctgtgcgatg accaccgaga tgggaccgcg cgccgtactg cgtgaagata ccattccgtt
     1861 tcttgaggca ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg ctcatccgca
     1921 caacctggcg gtaaaacttg agcatacggg tctggacgca caccttgatt tattactttc
     1981 cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg tggccgaagc
     2041 tacgggtctg aaagctgaaa gaacgctgtt tattgatgac agcgaagcga ttctcgatgc
     2101 tgccgcgcaa tttggtattc gttactgcct cggcgtgact aatcctgatt ccgggattgc
     2161 cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga tcccctcgct
     2221 aatgtgaagg agacgccatg aaagagaaac ctgctgttga ggttcgactg gataaatggc
     2281 tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta
     2341 aggtgcatta caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg aatgccacgc
     2401 tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt actgaacagc
     2461 gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt gtagagaaac
     2521 gcgaaaaaat ggcgatggca cgtaaactta atgccttaac catgccgcac ccggaccgac
     2581 gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac agtgaataac
     2641 tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc tatctgtttg
     2701 aaaattttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa cagatccttg
     2761 agaaccacga ttatccgcag cccgttaaaa acgtgctggc agaactgctg gttgcgacca
     2821 gcctgttaac cgctacgctg aagtttgatg gtgatatcac cgtacagctg cagggcgacg
     2881 gtccgatgaa tctggcggtt attaacggta acaataacca gcaaatgcgc ggtgtggcgc
     2941 gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc aatggttacg
     3001 tggtgatcac cattaccccg agcgaaggcg aacgctatca gggcgtagtt ggtctggaag
     3061 gtgataccct ggcggcctgc ctagaagatt actttatgcg ttctgaacag ctgccgacgc
     3121 gcctgtttat tcgcgccggc gacgtagacg gcaaaccggc tgcaggcggt atgttgttgc
     3181 aggtaatgcc tgcgcaaaat acccaacagg acgactttga ccacctggcg acgctaaccg
     3241 aaaccatcaa aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg tggcgtttgt
     3301 atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct
     3361 cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt gatagcatcc
     3421 tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac tatctgttca
     3481 atgcgatgga tattgctgaa atccgcaaca acgcgtctcc ggcagatccg taagttcatt
     3541 aatgagtatg tccggcagag agggctctgc cggattttac gacttaatgg cctgatgtgc
     3601 tgcgcttacc agcgcctatg aaataaatcg caaattattt tggtcagata cagcgtcgta
     3661 tccgataaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca tctaccgtca
     3721 gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct gtgagtaatt
     3781 tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt
     3841 ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg atccgctccg
     3901 cctgaccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt tcaatacagg
     3961 tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag
     4021 aaccaggggc aattaacaca ttttcggcga atactttttc cgatacctgt tcctgaagat
     4081 gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct ttcataaacc
     4141 cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc tgttcgagca
     4201 tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt
     4261 tcacgctaat acgcatactg tccagcaccg gcgaattaag aagcaccagt atcaccaaca
     4321 ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt tgcccattac
     4381 gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc tccgccagcg
     4441 cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacccccagg
     4501 tggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg caacgcaacg
     4561 gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt aagatcaatt
     4621 gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa atcgcgctca
     4681 ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact aacggcagca
     4741 accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt gactgtgtac
     4801 gcgcgaggat caccgccagt gcggtaacca gaccaaatgt aagatacata aaccagtcgg
     4861 ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac tcaccccaca
     4921 gtaaaagcac cagccacgtc aggccattgg agagaaaaat caccagtaat ataagtacgt
     4981 tatgccatac tgactggtaa aagtagctat agcggctact gccattgcgg atacgaaggc
     5041 tttgttgtat ccacggtagc aacaacattg ccatcaacag cagatagcag ccaaaatccc
     5101 aaatcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc agccaaccgc
     5161 tcatccctgt cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa
     5221 aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa
     5281 caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa accagcccct
     5341 gaagtaatcc ggtagcaatc atcccctgaa gtaatccggt agcaatcatc ccccagcgtg
     5401 ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat
     5461 tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt
     5521 ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat gcgattccac
     5581 tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta
     5641 aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc
     5701 aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa
     5761 aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa
     5821 tggtttgccc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta
     5881 caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga
     5941 gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg
     6001 ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc
     6061 agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct ggcagcatct
     6121 gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg acgctttctg
     6181 tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc
     6241 gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag gtttcaaacc
     6301 agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct
     6361 gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga ttggcggcac
     6421 ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct
     6481 gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg atgttgcggt
     6541 gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga aacgccgcct
     6601 gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaatttcg aaggcggctg
     6661 ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg
     6721 tcgtgatgcg ctgctagaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga
     6781 tggttcaaaa accgagaaca cccgcgtttc ttatccaatc tatcacatcg ataacatcgt
     6841 taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg ctgatgcttt
     6901 cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc acttcctctc
     6961 tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga cgccaacctt
     7021 ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg cagaagtgct
     7081 ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg gctggaacgg
     7141 cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg
     7201 ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga tcccaaccga
     7261 actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt ctccggaaca
     7321 gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg ataaatacac
     7381 cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat gatttgaagc
     7441 tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt
     7501 ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc agccaggcgc
     7561 gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta tggttatcca
     7621 cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc
     7681 cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc ggaccgtcat
     7741 cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg atccagccat
     7801 tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc gacagcgggt
     7861 gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc tcatagccac
     7921 tttcggcagc aatcacctca ccgagcactg cattaagatc cgccatttcc atcggcatct
     7981 cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac tcttcgatat
     8041 ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca gtcgccaggc
     8101 gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt
     8161 catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta acggaacgca
     8221 cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttacca acctgcaagg
     8281 ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc cacgccccgc
     8341 ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa tcgccctgat
     8401 gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt ttcagccaga
     8461 cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc agttgctgtg
     8521 ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag
     8581 cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag
     8641 gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt
     8701 cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca
     8761 ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg
     8821 caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa
     8881 gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat cttcttctac
     8941 catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg
     9001 accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag
     9061 tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt cgcggaacat
     9121 ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga
     9181 cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac gggccagcag
     9241 ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat
     9301 acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt tgctctgact
     9361 acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat ccagtaccat
     9421 aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg
     9481 aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc
     9541 atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag gttcgcaaca
     9601 atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa
     9661 tttcagccta aattgttaca aagcatatta aacagcagct taagtataca atttattcgg
     9721 cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac
     9781 agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac
     9841 tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga
     9901 cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc
     9961 gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa
    10021 tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg gtggagattg
    10081 aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc
    10141 gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg
    10201 gcgatctggc ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt aatgctatcg
    10261 agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag
    10321 ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga
    10381 atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa caggttgacg
    10441 ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg
    10501 aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc
    10561 tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc catttccgag caaggcaaac
    10621 tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc
    10681 tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt gaagcagggc
    10741 ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg
    10801 cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc
    10861 gctatatcct gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt
    10921 atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa gaggaagggg
    10981 cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg
    11041 cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg aatgccgatc
    11101 cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg gatcaccttg
    11161 gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg agctggacct
    11221 ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg
    11281 cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggcggccc
    11341 ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg
    11401 cggtggtcga tgccactggc aaactggtgg cgaccgatac catttacccg cacaccggac
    11461 aagccgcaaa agcagcgatg accgttgctg ccttgtgtga aaaacataac gttgaactgg
    11521 tggcgatcgg caacggtaca gcttcccgcg aaactgaacg tttctatctc gacgtgcaga
    11581 agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt
    11641 actcagcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg ctgcgtggtg
    11701 cggtatctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga
    11761 aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg gcccgcaaac
    11821 tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg
    11881 ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct
    11941 ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc agccgtctgg
    12001 ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt gataacccgc
    12061 tggacgcctc taccgttcat ccggaagcct atccggtggt ggaacgcatt ctggcagcaa
    12121 cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcgt
    12181 ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg
    12241 aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg
    12301 agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc aacgtcacca
    12361 actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac atctcttcat
    12421 tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg
    12481 tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg cgtctggacg
    12541 agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg caaaacaacc
    12601 gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc gcgatgatgg
    12661 atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg
    12721 cgcgataagg cgttcacgcc gcatctggca acgaacgctt tgcctgacat aaaagtgccg
    12781 gagaatatct ccggcacttt tattccacag ccaaactcac aatatactcc gttaatattt
    12841 atcacttcat taacaactga aaccttaatt aaacattagc cagtccgggt aactcactat
    12901 tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag tcagattatt
    12961 attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt ctcattatca
    13021 ttgtgttgtt gattatttaa tctctccttt gttggcaaat catctggtct catgtcgcgg
    13081 tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata
    13141 cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc cggcatatcg
    13201 ccaaaaactg ctttctctag gcatgttacc tggctcctct tttgatgtgg tgcgcgtcgc
    13261 tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa
    13321 agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga
    13381 aaaaattaac cattggctta attggtaatc caaattctgg caagacaacc ttatttaacc
    13441 agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag
    13501 aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt
    13561 ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt
    13621 tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc
    13681 tgtacctgac gctgcaactg ctggaactcg gcattccctg catcgtggcg ctgaacatgc
    13741 tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctatcg gcacgtcttg
    13801 gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg
    13861 cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca cagccgctac
    13921 tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc
    13981 gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag
    14041 cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa tgaaatggat gatccggcgc
    14101 tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca
    14161 ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt
    14221 tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg gctatcaaca
    14281 tcggcggggc gttacagccg ctgtttaacg tcggctccgt ggcgctattt gtgcatggta
    14341 ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccagg
    14401 gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc atgatgtacc
    14461 tgttcctctc cttccttgag gactccggct atatggcgcg tgcagcgttt gtgatggacc
    14521 gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc gtcggtttcg
    14581 gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga
    14641 tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat
    14701 tcgcggctgc cttcttcggg cagaacggtg ccctggcggt tttctcgctg tatatgctcg
    14761 gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag
    14821 cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa agcctgatta
    14881 tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg attatcatcg
    14941 tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc gtcgataaca
    15001 tcaacgactc ggcgctggct tccgtcagcc gggtgataac ccctgtcttc aagccgattg
    15061 gcgtacatga agataactgg caggcaacgg ttggcctgtt tacgggcgca atggcaaaag
    15121 aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt
    15181 tcaatccggc ggaattcaac ctcggtgaag agctgttcag tgcggtagat gaaacctggc
    15241 agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa gctagcaaag
    15301 gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag
    15361 ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc tcggtgatgg
    15421 gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga
    15481 atatcgctta ctcactggca acattgttct atcaggtcgc cagctacagt cagcacccaa
    15541 cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct atcggtctgc
    15601 tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag tcggtaagca
    15661 gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta ttcaggtacg
    15721 tgatttgctg gcgttacggg gccgtatgga agcgacccag ataagccaga cattgaacac
    15781 tccgcagcca atgattaacg ccatgctgca acagctggaa agcatgggca aagccgtgcg
    15841 gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc cggaaggcaa
    15901 agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa
    15961 gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa aatgcaattc
    16021 ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca
    16081 cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg aacgccatga
    16141 gtaaaaagca gagttccacc ccacatgatg cgctgttcaa actcttttta cgccaaccgg
    16201 agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg ctctgtgata
    16261 tgaaaaccct caagctggag tcgaggagct ttattgatga cgatctgcgt gaaagctatt
    16321 ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat tgtctgattg
    16381 aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac gctattgccg
    16441 caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg ccattgttgt
    16501 tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat tgtttcgccg
    16561 atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat gtcaccgtca
    16621 tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta attcaaaaac
    16681 atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta ttaagtagtg
    16741 gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa actggcgacg
    16801 ctgtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga
    16861 gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa gccctgcata
    16921 tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcga tttaccgggc
    16981 tgtcagaaga agagttggct gcggcgagta gactggcccc ctgaatctcc agacaaccag
    17041 tatcacttaa
//
LOCUS       DAJTMX010000089        16388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTMX010000089 DAJTMX010000000
VERSION     DAJTMX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.089"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO145_002826"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO145_002826"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5467885.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO145_002827"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO145_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5467886.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO145_002828"
     CDS             2339..2554
                     /locus_tag="OO145_002828"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467887.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO145_002829"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO145_002829"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5467888.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO145_002830"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO145_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5467889.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO145_002831"
     CDS             5920..6417
                     /locus_tag="OO145_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5467890.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO145_002832"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO145_002832"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5467891.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO145_002833"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO145_002833"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5467892.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO145_002834"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO145_002834"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5467893.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO145_002835"
     CDS             9102..9665
                     /locus_tag="OO145_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5467894.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO145_002836"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO145_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5467895.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO145_002837"
     CDS             complement(10257..11237)
                     /locus_tag="OO145_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5467896.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO145_002838"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO145_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5467897.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO145_002839"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO145_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5467898.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO145_002840"
     CDS             12540..12830
                     /locus_tag="OO145_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5467899.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO145_002841"
     CDS             12838..13431
                     /locus_tag="OO145_002841"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5467900.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO145_002842"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO145_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5467901.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14873..15836
                     /locus_tag="OO145_002843"
                     /pseudo
     CDS             14873..15836
                     /locus_tag="OO145_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15881..16384
                     /locus_tag="OO145_002844"
     CDS             15881..16384
                     /locus_tag="OO145_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5467902.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4060 a         4220 c         4123 g         3985 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactaat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt ttatgaaaat
    14881 gaggtcattt acccgctcat tagtctgcgc atccttgctg gctctggtta gcactggcgt
    14941 aaacgccgca gaaaaagtgg cgttaaagtt agcacacaac ctggaaagaa gtcatgttgt
    15001 acatcagagt tttgaagagc tggcgaaaga ggtaaaacag ctgtcaaaag gtaatatggt
    15061 gatccgtatt tatcccagca gccagatggg taatgcgcga gaaactatgg aattgttgca
    15121 aaatggcgca ctggatatga ccaaaggttc cgccagtgat ctggagtctt ttgataatat
    15181 ttatgctatc tataatttgc cgttcttatt aagttgtttt tggtgaagtt ggtaaagaaa
    15241 ttatggactc aacaaaagac aaaggctttt ttgctttatc agcttatgta gcgggtactc
    15301 gcagtttcta tgcgaaaaaa ccgattacaa agccagaaga tcttaaagga ctgaaaattc
    15361 gtgttcagcc aagtccgacc accatcaaaa tgattgaact gatgggtggt tcacccactc
    15421 caatttcatt tggcgaagtt tatactgcga tgcagcaagg tgtagtagat ggcgcagaaa
    15481 ataacgtgcc ttcctggatg cagactcgac atattgaaat tgccaaagtt ttttctgaag
    15541 atgaacacgc ctcaattcct gactttttgg ttatctccac caaaacatgg aataaattaa
    15601 ccccagaaca gcaacaaatc ctcgaaacag cggcgaagaa atccgaagct tatcaacaaa
    15661 aattgtggga aaaaattgac gctgacacac gtgctcaggc gaaagctatg gggggtgaaa
    15721 ttgtaaaagt ggataaagcg ccattccgta aagccgtgca acctctgttt gacgacttca
    15781 aaaaagatcc aaaacaagct gctttgttag agaaattcga caacgctgct caataatatt
    15841 ccgggccagc ctctggccct gattttccag gatgcttaca atgatattta accgcctgaa
    15901 acttgcggta gaccgcgtca ttgccacgtt ttctgtcgcc gtaatgcttg ctctggttgt
    15961 atgcgtagtc tggcaagtat ttagccgcta cgtacttaac cagccaagta cattaaccga
    16021 tgagctggca cgctttttga tgatctgggt aggattatta ggtgctgcat ataccgtcgg
    16081 cgcgcagcga catctctcta ttgatttatt tgctctcgcc ctgaataagc gcaaacaatt
    16141 acttcttagt atagtcatta acgttctcat tttaggtttt gccggttcgg tcattgtgac
    16201 tggtggcctg aaattaattg ataaaacact ggcaacatca caggtatctg ccgctatgca
    16261 aataccgatg ggatacgtat atatcattct gccattaagt ggagtggtaa tgatgtttta
    16321 tgccttgtgt tttattaatc aaagcattca acaactgaaa caacctgcgc aggaggccag
    16381 ttaatgga
//
LOCUS       DAJTMX010000090        16340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTMX010000090 DAJTMX010000000
VERSION     DAJTMX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.090"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1100)
                     /locus_tag="OO145_002845"
     CDS             complement(<1..1100)
                     /locus_tag="OO145_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5467903.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO145_002846"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO145_002846"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5467904.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO145_002847"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO145_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5467905.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO145_002848"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO145_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5467906.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO145_002849"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO145_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5467907.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO145_002850"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO145_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5467908.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO145_002851"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO145_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5467909.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO145_002852"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO145_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5467910.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO145_002853"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO145_002853"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5467911.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO145_002854"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO145_002854"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5467912.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO145_002855"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO145_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5467913.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO145_002856"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO145_002856"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5467914.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OO145_002857"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OO145_002857"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5467915.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OO145_002858"
     CDS             11850..12014
                     /locus_tag="OO145_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467916.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OO145_002859"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OO145_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5467917.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OO145_002860"
     CDS             13523..13768
                     /locus_tag="OO145_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5467918.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OO145_002861"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OO145_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5467919.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..>16340
                     /locus_tag="OO145_002862"
     CDS             15721..>16340
                     /locus_tag="OO145_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5467920.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRIST"
BASE COUNT         3702 a         4329 c         4221 g         4088 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat
//
LOCUS       DAJTMX010000091        15977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTMX010000091 DAJTMX010000000
VERSION     DAJTMX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.091"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..938
                     /gene="dnaE"
                     /locus_tag="OO145_002863"
     CDS             <1..938
                     /gene="dnaE"
                     /locus_tag="OO145_002863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time; main
                     replicative polymerase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5467921.1"
                     /translation="IEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGP
                     HRAALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLD
                     GERETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTK
                     RGNRIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTA
                     REVMDIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADAR
                     ARLRFGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            951..1910
                     /gene="accA"
                     /locus_tag="OO145_002864"
     CDS             951..1910
                     /gene="accA"
                     /locus_tag="OO145_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5467922.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2009..4150
                     /gene="ldcC"
                     /locus_tag="OO145_002865"
     CDS             2009..4150
                     /gene="ldcC"
                     /locus_tag="OO145_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5467923.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4207..4596
                     /locus_tag="OO145_002866"
     CDS             4207..4596
                     /locus_tag="OO145_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5467924.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4661..5959
                     /gene="tilS"
                     /locus_tag="OO145_002867"
     CDS             4661..5959
                     /gene="tilS"
                     /locus_tag="OO145_002867"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5467925.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6008..6268)
                     /gene="rof"
                     /locus_tag="OO145_002868"
     CDS             complement(6008..6268)
                     /gene="rof"
                     /locus_tag="OO145_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5467926.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6255..6455)
                     /locus_tag="OO145_002869"
     CDS             complement(6255..6455)
                     /locus_tag="OO145_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5467927.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6621..7166
                     /locus_tag="OO145_002870"
     CDS             6621..7166
                     /locus_tag="OO145_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5467928.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7163..7585
                     /gene="arfB"
                     /locus_tag="OO145_002871"
     CDS             7163..7585
                     /gene="arfB"
                     /locus_tag="OO145_002871"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5467929.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7599..8309
                     /gene="nlpE"
                     /locus_tag="OO145_002872"
                     /gene_synonym="cutF"
     CDS             7599..8309
                     /gene="nlpE"
                     /locus_tag="OO145_002872"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5467930.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8464..9288)
                     /locus_tag="OO145_002873"
     CDS             complement(8464..9288)
                     /locus_tag="OO145_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5467931.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9341..11059)
                     /gene="proS"
                     /locus_tag="OO145_002874"
     CDS             complement(9341..11059)
                     /gene="proS"
                     /locus_tag="OO145_002874"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5467932.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11170..11877)
                     /gene="tsaA"
                     /locus_tag="OO145_002875"
     CDS             complement(11170..11877)
                     /gene="tsaA"
                     /locus_tag="OO145_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5467933.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(11874..12278)
                     /gene="rcsF"
                     /locus_tag="OO145_002876"
     CDS             complement(11874..12278)
                     /gene="rcsF"
                     /locus_tag="OO145_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5467934.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12396..13211)
                     /gene="metQ"
                     /locus_tag="OO145_002877"
     CDS             complement(12396..13211)
                     /gene="metQ"
                     /locus_tag="OO145_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5467935.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13251..13904)
                     /gene="metI"
                     /locus_tag="OO145_002878"
     CDS             complement(13251..13904)
                     /gene="metI"
                     /locus_tag="OO145_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5467936.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(13897..14928)
                     /gene="metN"
                     /locus_tag="OO145_002879"
     CDS             complement(13897..14928)
                     /gene="metN"
                     /locus_tag="OO145_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5467937.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15116..15691
                     /gene="gmhB"
                     /locus_tag="OO145_002880"
     CDS             15116..15691
                     /gene="gmhB"
                     /locus_tag="OO145_002880"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5467938.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3893 a         4018 c         4267 g         3799 t
ORIGIN      
        1 tcatcgaagc ccgtaataaa ggcggctact tccgcgaact gtttgatctc tgcgcccgta
       61 ccgacaccaa aaagttaaac cggcgggtgc tggaaaaact gatcatgtcc ggggcgtttg
      121 accgtcttgg gccacaccgc gcggcgctga tgaactcgct gggtgatgcg ttaaaagcgg
      181 cagatcaaca cgcgaaagcg gaagctatcg gtcaggccga tatgttcggc gtgctggccg
      241 aagagccgga acaaattgaa caatcctacg ccagctgcca accgtggccg gagcaggtgg
      301 tattagatgg ggaacgtgaa acgttaggcc tgtacctgac cggacaccct atcaaccagt
      361 atttaaaaga gattgagcgt tatgtcggag gcgtaaggct gaaagacatg cacccgacag
      421 aacgtggtaa agtcatcacg gctgcggggc tcgttgttgc cgcgcgggtt atggtcacca
      481 agcgcggcaa tcgtatcggt atctgcacgc tggatgaccg ttccgggcgg ctggaagtga
      541 tgttgtttac tgacgccctg gataaatacc agcaattgct ggaaaaagac cgcatactta
      601 tcgtcagcgg acaggtcagc tttgatgact tcagcggtgg gcttaaaatg accgctcgcg
      661 aagtgatgga tattgacgaa gcccgggaaa aatatgctcg cgggcttgct atctcgctga
      721 cggacaggca aattgatgac cagcttttaa accgactccg tcagtctctg gaaccccacc
      781 gctctgggac aattccagta catctctact atcagagggc ggatgcacgc gcgcggttgc
      841 gttttggcgc gacgtggcgt gtctctccga gcgatcgttt attaaacgat ctccgtggcc
      901 tcattggttc ggagcaggtg gaactggagt ttgactaata caggaatact atgagtctga
      961 atttccttga ttttgaacag ccgattgcag agctggaagc gaaaatcgat tctctgactg
     1021 cggttagccg tcaggatgag aaactggata ttaacatcga tgaagaagtg catcgtctgc
     1081 gtgaaaaaag cgtagaactg acgcgtaaaa tcttcgccga tctcggtgca tggcagattg
     1141 cgcaactggc acgccatccg cagcgtcctt ataccctgga ttacgttcgc ctggcatttg
     1201 atgaatttga cgaactggct ggcgaccgtg cgtatgcaga cgataaagct atcgtcggtg
     1261 gtatcgcccg tctcgatggt cgtccggtga tgatcattgg tcatcaaaaa ggtcgtgaaa
     1321 ccaaagaaaa aattcgccgt aactttggta tgccagcgcc agaaggttac cgcaaagcac
     1381 tgcgtctgat gcaaatggct gaacgcttta agatgcctat catcaccttt atcgacaccc
     1441 cgggggctta tcctggcgtg ggcgcagaag agcgtggtca gtctgaagcc attgcacgca
     1501 acctgcgtga aatgtctcgc cttggcgtac cggtagtttg tacggttatc ggtgaaggtg
     1561 gttctggcgg tgcgctggcg attggcgtgg gcgataaagt gaatatgctg caatacagca
     1621 cctattccgt tatctcgccg gaaggttgtg cgtccattct gtggaagagc gccgacaaag
     1681 cgccgctggc ggctgaagcg atgggtatca tcgctccgcg tctgaaagag ctgaaactga
     1741 tcgactccat catcccggaa ccgctgggcg gcgctcaccg taacccggaa gcgatggcgg
     1801 catcgttgaa agcgcaactg ctggcggatc tggccgatct cgatgtgtta agcactgaag
     1861 atttaaaaaa tcgtcgttat cagcgcctga tgagctacgg ttacgcgtaa ttcgcaaaag
     1921 ttctgaaaaa gggtcacttc ggtggccctt ttttatcgcc acggtttgag caggctatga
     1981 ttaaggaagg attttccagg aggaacacat gaacatcatt gccattatgg gaccgcatgg
     2041 cgtcttttat aaagatgagc ccatcaaaga actggagtcg gcgctggtgg cgcaaggctt
     2101 tcagattatc tggccacaaa acagcgttga tttgctgaaa tttatcgaac ataaccctcg
     2161 aatttgcggc gtgatttttg actgggatga gtacagtctc gatttatgca gcgatatcaa
     2221 tcaacttaat gaatatctcc cgctttatgc cttcatcaac acccactcga cgatggatgt
     2281 cagcgtgcag gatatgcgga tggcgctctg gttttttgaa tatgcgctgg ggcaggcgga
     2341 agatatcgcc attcgtatgc gtcagtacac caacgaatat cttgataaca ttacgccgcc
     2401 gttcacgaaa gccttgttta cctacgtcaa agagcggaag tacacctttt gtacgccggg
     2461 gcatatgggc ggcaccgcat atcaaaaaag cccggttggc tgtctgtttt atgatttttt
     2521 cggcgggaat acccttaagg ctgatgtctc tatttcggtc accgagcttg gttcgttgct
     2581 tgaccacacc gggccacacc tggaagcgga agagtacatc gcgcggactt ttggcgcgga
     2641 acagagttat atcgttacca acggaatatc gacgtcgaac aaaattgtgg gtatgtacgc
     2701 cgcgccatcc ggcagtacgc tgttgatcga ccgcaactgc cataaatcac tggcgcatct
     2761 gttgatgatg aacgatgtag tgccagtctg gctgaaaccg acgcgtaatg cgttggggat
     2821 tctggggggg atcccgcgcc gtgaatttac tcgcgacagc atcgaagaga aagtcgctgc
     2881 cactacgcaa gcacaatggc cggttcatgc ggtgatcacc aactccacct atgatggttt
     2941 gctctacaac accgactgga tcaaacagac gctggatgtc ccgtcgatcc acttcgattc
     3001 cgcctgggtg ccgtatactc attttcatcc aatctaccag ggtaaaagtg gtatgagcgg
     3061 cgagcgtgtt gcgggaaaag ttttcttcga aacgcaatcg acccacaaaa tgctggcggc
     3121 gttatcgcag gcttcactga tccacattaa aggcgagtat gacgaagagg cgtttaacga
     3181 agcctttatg atgcatacca ccacctcgcc cagttatccc attgttgctt cggttaagac
     3241 ggcggcggcg atgctgcgtg gtaatccggg caaacggctg attaaccgtt cggtggagcg
     3301 ggcgctgcat tttcgcaaag aggtccagcg gctgcgggaa gagtctgacg gttggttttt
     3361 cgatatctgg caaccgccgc aggtggatga agccgaatgc aggcccgttg cgcctggcga
     3421 acaatggcac ggctttaacg atgcggatgc cgatcatatg tttctcgatc cggttaaagt
     3481 cactattttg acaccgggga tggacgaaca gggcaatatg agtgaggagg ggatcccggc
     3541 ggcgctggta gcaaaattcc tcgacgaacg tgggatcgta gtagagaaaa ccggccctta
     3601 taacctgctg tttctcttta gtattggcat cgataaaacc aaagcaatgg gattattgcg
     3661 tgggttgacg gaatttaagc gctcttacga tctcaacctg cggatcaaaa atatgctgcc
     3721 cgatctctat gctgaagatc ccgatttcta ccgcaatatg cgtattcagg atctggcgca
     3781 agggatccat aagctgattc gtaaacacga tctttccggt ttgatgttgc gggcattcga
     3841 tactttgccg gaaatgatca tgacgccaca tcaggcatgg cagagacaga ttaaaggtga
     3901 agtagaaacc attgcgctgg aacaactggt cggtagagta tcggcaaata tgatcctgcc
     3961 ttatccaccg ggcgtaccgc tgttgatgcc aggagaaatg ctgaccaaag agagccgcac
     4021 ggtactcgat tttctactga tgctttgttc cgtagggcaa cattaccccg gttttgaaac
     4081 ggatattcac ggcgcgaaac aggacgaaga cggcgtttac cgcgtacgag tcctaaaaat
     4141 ggcgggataa cttgccagag cggcttccgg gcgagtaacg tgctgttaac aaataaagga
     4201 gacgttatgc tgggtttaaa acaggttcac catattgcga ttattgcgac ggattatgcg
     4261 gtgagcaaag ctttctactg cgatattctt ggtttcacgc tgcaaagcga agtctatcgc
     4321 gaagcgcgcg actcgtggaa aggggatttg gcgcttaatg ggcaatatgt gattgagctt
     4381 ttctcttttc cgttcccgcc ggagcgcccc agccgaccgg aagcttgcgg cctgcgtcat
     4441 ctggctttta gcgttgatga catcgatgcg gcagtggcgc accttgaaag ccataacgtg
     4501 aagtgtgaag ccatccgtgt cgatccatac acgcaaaaac gcttcacctt ctttaacgat
     4561 ccggacgggc tgccgttgga actgtatgag cagtaaggct tgtcatcgcc gcatttgccc
     4621 ggtaacgtgc cgggcattgc tactgtaaaa tcgcaccatc atgacactca cgctcaatag
     4681 acaacttctc acctcacgcc agattctggt ggcctttagc ggcgggcttg actccaccgt
     4741 tctgctgcat cagttggtgc agtggcggac ggaaaatccg ggcgtcactc tgcgcgctat
     4801 tcatgtgcat cacggtttaa gtgccaatgc cgatgcctgg gttacgcatt gcgaaaacgt
     4861 ctgccaacag tggcaggtgc cgctggtggt cgaacgcgta caacttgcgc aagaggggct
     4921 ggggattgag gcccaggcgc ggcaggcacg ttatcaggca tttgcccgta ccctattgcc
     4981 cggcgaagtg ctggtcaccg cgcaacatct cgacgatcaa tgtgaaacct ttctgctggc
     5041 gctaaaacgc ggtagcggcc ctgccgggct ttcggctatg gcggaagttt cggagtttgc
     5101 tggaacacgg cttatccgcc cgttgcttgc ccgcacgcgg ggggaactgg agcagtgggc
     5161 attggcgcat ggtttacgct ggattgaaga cgaaagtaat caggacgact catacgatcg
     5221 taactttctg cgcctgcgcg tagtgccgtt attgcagcag cgttggccgc attttgcgga
     5281 agcaacggcc cgcagcgcca cactttgtgc tgaacaagag agtctgttgg atgaactgct
     5341 ggcggatgat ttagcacact gccaaacgtc gcaggggacg ctgcagattg cgccaatgct
     5401 ggcgatgagt gatgcccgtc gcgcggcgat tattcgccgc tggctggcag ggcagaatgc
     5461 accgatgcct tcccgcgacg cgctggtgcg catctggcag gaagtggcgc tggcgcggga
     5521 agatgcttcc ccctgtttac gtttgggcgc ctttgaaatc cgccgctatc agtcgcaact
     5581 gtggtggatt aaatccgtca ccgggcaaag cgaaaccatt gtgctgtggc agacgtggct
     5641 acaaccgctg gaattaccgg cggggctggg aaccgtacag cttactgcgg gaggcgatat
     5701 tcgccctccg cgtgcagacg aagcggtcag cgtgcgtttc aaagcgccag gattgctgca
     5761 tattgtcggg cgcaacggcg gacgtaagct gaagaaaatc tggcaagagt taggcgtgcc
     5821 gccgtggcta cgtgacacca cgccactgct gttttatgga gaaacgctga ttgcggcggc
     5881 aggggtattt gtgacgcaag aaggtgtggc tgaaggtgag aatggcgtaa gttttgtctg
     5941 gcagaaaacg cttagttaag tgaaagccgg ataagacgca tcaaacgtcg catccggcga
     6001 aagtcaatca ggattcgctt accaccaccg taccgatttc agggtggcta aagctggtaa
     6061 ttttatccag acgcagctcg cgggtttcgc cagcggcctc gacgaccaga tactccacat
     6121 ttttgcggga gactaaatca ctggcttttg cctgcaattt ttcgccatct ttcagctcaa
     6181 gtgtcagcat taaatgatgc tggcaggcga gctcaagatt atcgtaatca tcacaattaa
     6241 ttggttgata cgtatcattc attgacataa tcgctcacca gtaagtttgc cgcagcgtat
     6301 gctgcttttt ccctgacagc ctcagaaagg gcgtcgtcgg cagccatttc attcagcact
     6361 ttcaaaacgc agcccagcgc gtccggaacg tatcctaagt ctccgctggc gatttccgcg
     6421 taccgcttgc gtattaactc acaatatttt tccacatgcc ctcctgtcag cactctgact
     6481 taaccgtgga tgcaagtcta agcctacgaa gataaactct gtttcgcaag gtgactatac
     6541 cacactcatt tctgcaatat cagcgccgca actgcacgta ttccgttaca atggcctcct
     6601 gattcgaaag gagttttctt atggcgctta aagcgacaat ttataaagcg acggttaatg
     6661 tggccgatct cgaccgcaac cagtttctcg atgcctctct gacgctggcg cgccaccctt
     6721 cagaaaccca ggagcgtatg atgctgcgct tgctggcgtg gctgaaatat gctgatgaac
     6781 gtctgcaatt tacccgtggt ttgtgtgccg atgatgagcc ggaagcgtgg ctgcgtaacg
     6841 atcacctggg cattgatttg tggattgagc tggggctgcc ggatgagcga cgaattaaga
     6901 aagcctgcac ccaggccgca gaagtggcgc tgtttaccta taatagtcgg gcggcgcaaa
     6961 tctggtggca gcaaaatcag agcaaatgtg tgcagtttgc caatctttcc gtctggtatc
     7021 tggacgatga acaactggcg aaagtaagcg cctttgccga tcgtaccatg acgctgcagg
     7081 caacgattca ggatggtgtt atctggttat cggatgataa gaataatctg gaagttaact
     7141 taaccgcctg gcaacaacct tcatgattgt gatttcccga catgttgcta ttcccgatgg
     7201 tgagcttgag atcaccgcca ttcgtgcgca gggcgctggc gggcagcatg ttaataagac
     7261 ctcaacggct attcatctgc gttttgacat tcgggcgtcc agcctgccag agtattacaa
     7321 agagcgtctg ctcgccgcca gccatcattt gatcagcagt gatggcgtga ttgtcattaa
     7381 ggcacaggaa taccgcagtc aggaactgaa ccgcgaagcc gctctggccc ggctggtggc
     7441 agtgattaaa gatttaacaa aagaacaaaa agcccgacga cccacgcggc ccacccgtgc
     7501 atcgaaagag cgcaggctgg catcgaaagc acaaaaatca agcgtgaagg cgatgcgcgg
     7561 caaagtacgc agcggtcggg aataaaaaga aggaatggat ggtgaaaaaa gcgatagtga
     7621 cagcgatggc tgtaatcagc ctctttactc tgatgggatg taataatcgg gccgaagtcg
     7681 atacgctttc tccggcgcag gctgccgaac tgaaaccgat gccgcaaagt tggcgcggcg
     7741 tgctaccgtg tgccgattgc gaaggaatcg aaatctctct gttcctcgaa aaagacggaa
     7801 catgggtgat gaatgagcgt tatctcggtg ctcgtgaaga accttcctcc ttcgcttcct
     7861 acggtacatg ggcgcgaacc gctgacaagc tggtattaac cgacagcaaa ggtgaaaagt
     7921 catattatcg ggcgaaaggc gatgcgctgg agatgctcga tcgtgaaggc aatccaattg
     7981 aatcgcagtt caactatacg ctggaaccgg cacaatccag tttacctatg acgccgatga
     8041 ccctgcgagg catgtatttt tatatggctg atgcggcgac cttcactgat tgcgcgaccg
     8101 gaaaacgttt catggtagcg aataacgcag agctggagcg tagctacctg gctgcgcgcg
     8161 gtcacagtga aaaaccggtg ttactgtcag tagaaggtca ctttacgctt gaggctaatc
     8221 cggatactgg tgcgccaacc aaagtattag cgcccgatac ggcgggtaaa ttttacccga
     8281 ataaggattg cagtagtttg gggcagtaaa cctgcttaag gcagaaacaa acgcaaaatt
     8341 gcctgatgcg ctgcgcttat caggcctaca gcatataatg caacattttg aattagcacg
     8401 attttgtagg ccggataagg cgtttacgcc gcatccggca ttttgtgcac gaggctggct
     8461 ttatcactga gtcagcccaa caaagcgacc cgccttgata taaatcccag gcttcaggtg
     8521 cccaacatac tgtaactgcg tttctggctt aaacgcagag acatcaccaa tacgcatatg
     8581 catcagatcg gcagggctaa tccagcccga ttgcgccagt gtaagtggat gcccggattt
     8641 ggcaaatgca tcagtgacaa attccgaaca aaaccacgac tttttgtctc cttcgcacac
     8701 actgcttaac tgcgctttcg ccaggtcgct gacgcactgt tggcgaaaat cctcggagaa
     8761 cggattcagt gagcacatct ggcgagtcac cataaaggga ataaattcga caatgccgcg
     8821 atagttataa ccgctatctt tgattttatt ggcaaacgcg gtgatatctg tggcttgttg
     8881 cggggtaaga tccgggactc gtaagacgaa aagcttatca ctatgcttca tcgctttttt
     8941 aagggaaaca atctggacgc cagcgcctgt cgcttctgca acgttattat cacccagaaa
     9001 gattgcaacg tgactcacag aggaagtgct gaagacgcgg attccaaatg aggttacccc
     9061 aaggcttgag gagaacagca aatcgccggg tttgagatca ggtgccgtta tttcttttat
     9121 tgattgttcg gtgaaagagc tttgatgctg gaatttgaca gcccatgttt ttgcctcggc
     9181 atctactgct gtggctgatg ggtctggctg gctgatatca acggtacaag ccgaaagtaa
     9241 aagaaagctg gggagaagca ggcggcagta cgcctttggt ttatccattt tatacaatcc
     9301 atgtaaaaaa gggccctgaa attcaggacc ctttctggca tcagccttta atctgtttca
     9361 ccagatattc gacgatgtca ccagtcttaa ttaactgttt ctcgccgttg cgacgatatt
     9421 tatattcgat atcgtcgttg tcgaggttac ggtcgcccag cacaatagtg tgcggaatac
     9481 cgatcagttc catatcagca aacatcacgc ccggacgctc tttgcggtca tccagcagca
     9541 cttcgatacc ttgtgcacgc agttcgctgt acagtttctc agcaagctct tgtacgcgga
     9601 aggatttgtg catgttcatc ggcagaatcg ccacctggaa cggcgcgata gcgtcaggcc
     9661 atacgatgcc gcgttcgtcg tagttctgct caatcgccgc agctaccaca cgcgttaccc
     9721 cgataccgta gcaacccatc gtcaggattt ggttacggcc atcttcaccc tgtacggagg
     9781 ctttcagtgc ttcggagtac ttggtaccca gctggaagat gtgaccaact tcgataccac
     9841 gtttgatcag cagcgtaccc tggccatccg ggcttggatc gccagccacc acgttacgga
     9901 tatctgcaac ttccggggta gcgacatcgc gatcccagtt gataccgaag tagtgtttac
     9961 catcgatgtt agcaccagca gcgaaatcac tcatcgccgc aacggtacgg tcaatcacca
    10021 ccggaatcgg catgtttacc ggacccagtg aacccggacc ggctttaacc acagcacgaa
    10081 tttcttcttc ggtcgcgaaa gtcagcgggc ttgcaacctg cggcagtttt tctgctttaa
    10141 cttcgttcag ctcgtgatca ccgcgcacca gcagcgcaac cagcgggaag ctactgcctt
    10201 caaccgcttt aaccagcaga gtcttaaccg ttttctcaat cggcagattg aactgttcaa
    10261 ccagttccgc gatggttttc gcgttcggcg tatcaaccag cgtcatttcc tgggtagcag
    10321 cagcgcgcgg ttctttcggc gcgatagctt ctgccagttc aatgttcgct gcatagtcag
    10381 aggtgtcgga gaagaccaca tcgtcttcac cgctctgcgc cagcacctgg aattcgtgag
    10441 aggcgctgcc gccgatagaa ccggtgtcgg cctgtacggc gcggaaatcc agccccatgc
    10501 ggctgaagat tttgctgtag gccgcataca ttgcatcgta ggtttcctgc agggattcct
    10561 gagaagtatg gaaagagtaa gcatctttca tcaggaattc gcgggaacgc atgacgccga
    10621 aacgcggacg cacttcgtcg cggaacttgg tctggatctg atagaagttc agcggcagct
    10681 gtttgtaaga gctaagctcg ttacgaatca ggtcagtgat aacttcttca tgagttgggc
    10741 cgagtacgaa cggacgctcg ccacggtcaa caaaacgcag cagttccgga ccgtactgtt
    10801 cccaacgacc actctcttgc cacaaatcgg ctggctgaac caccggcatc gacacctcga
    10861 tcgcaccggc gttgttcatc tcttcacgca cgatgttttc gacttttttc agaacgcgca
    10921 cgccggtcgg cagccaggta tataacccgg aggccagctt gcggatcatc ccggcgcgca
    10981 gcatcagctg atggctgatc acctcggcgt cggcaggtgt ctccttgaga gtggagagca
    11041 ggtattggct agtacgcatg ttgttacggt tccagttgga aggtagaaca ggctcaaggc
    11101 gagcctggga caaaaaaagt gatttagttt accagtgcaa aagaaatgtc aaaagagaag
    11161 ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca
    11221 gcgaacgtta aaatcatgta gccagacggc ataggttttg cccgtttcct cacctttacg
    11281 ataggccggg cgcgggtcct gcgccagtac ttcgcggata aacagcgtta actgcggata
    11341 acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg gtaaaactca ccgccatctc
    11401 tgcagctggc gcactttgcg catagctggc actggcatcg ggaagcgatt cggcaaaggg
    11461 gagatacggt ttgatatcca ctaccggcgt accatcgacc agatccagac tgccgagctt
    11521 cagaatcacg ctgcctttat ggcaaacaac ctctttcagc tctaccagcg acatgccaat
    11581 tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc attctggcgt taccgccgag
    11641 gcgcggtgga cgcacagtcg gacgccagcc gccttccatc gtttgatgaa agacgaaaag
    11701 gatccataaa tggctgaatg cttccaggcc gcgaacggcg tcggcctggt tgtaggaagc
    11761 tatgagatgc agttcgccgt tggcgctttt taccagacct ggctggcgcg gaacggcgaa
    11821 tttttcttta tagggcgagc gaataacgcc tatttgctcg aactggaaac tgctcatttc
    11881 gccgtaatgt taagcgcaga gccgatacat acagcctgac gatagcagcc cggcgtaccg
    11941 ctggtgactt cgcagctatg cagtaataca gcattggctt tcattttaga ggcattgatt
    12001 tgcatctgct tacgtgcggt tggaatgctc ggcggagagt cctgattaga ggcctggcaa
    12061 gagtcgccac tgacttcacc gagatcgcgg aacggtttgc cgactaattc ttctgcattg
    12121 gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt ttgcaggctc cgctttcggc
    12181 tggggtgcag tgctttgaac gggttcgaca ggggatctgc ttaacatgga acagccgctt
    12241 agcatgagtg ctactaaaca gatcggtaaa gcacgcatag tatttcctca atgtatgatc
    12301 aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct
    12361 gaatgatatt acaaattgtg gaaacagcct aaaaattacc agcctttaac agcgccgccg
    12421 ttaaacactt tgtttgctgc ttcgtaaact tcatcagact gataagcctg gacgaatttc
    12481 ttcacgttct cggcgtcttt gttatcttca cgcgttacga tcaggtttac gtatggggag
    12541 tctttatctt cgacaaagat accgtctttc gccggagtca ggccaatctg gctggcatag
    12601 gtggtattga taactgccag agcgatttgc gcgtcgtcca gagaacgcgg cagttgcggt
    12661 gcttccagtt caacaatttt cagatttttt gggttctcaa caacatcaag aacggtcggc
    12721 agcaggccaa cgccatcttt cagtttgatc aagcccactt tttgcagcag cagcagtgaa
    12781 cgaccaagat tagttggatc gtttggcacg gcaacctgcg aaccatcctg cagttcatcc
    12841 agtgatttga ttttcttgga gtaaccagca atcggataaa caaaagtgtt gcctactgcg
    12901 accagtttgt agccacgatc tttcagttgc tgatcaaggt acggtttatg ctggaaggcg
    12961 ttggcgtcga tatcgccttt gctcaatgct tcgtttggca gaacatagtc gttgaaggtt
    13021 accagctcaa cgtccaggcc atatttgtct ttcgcaactt tctgcgcgac ttctgctacc
    13081 tgctgttcgg caccaacaat cacgccgact ttaatgtggt ttggatcttt ttcatcctga
    13141 ccgcagccta ccagtgccag tgatccaatc agggctccca ctgccgcaaa ggttttgaat
    13201 ttgaacgcca taccttattc cttcttcaat tatttatgtt gtgttgaacg ttacttgcga
    13261 gtgacagccc ggacgatgcg gtcgcctgca aactgaatta aataaaccag aatgaccagc
    13321 aataccagta ccgtattcat caccgtcgcg ttgtagccga tgtagccata ctgatagcca
    13381 atctgaccta aaccaccggc accgactgca ccacccatcg cggaataacc gaccagggta
    13441 atcagggtga tagttgccgc attcaccaga cccggcaacg cttccggtaa cagcacttta
    13501 cagacgatct gcattggcgt ggcacccatt gcgcgggaag cttcaattaa cccggttggg
    13561 atctccagca gagcgttctc gaccatacgg gcaataaacg gtgctgcacc aacggttaac
    13621 ggaacaattg ctgcctgcaa accaatcgat gtaccgacaa taacgcgggt aaacggaatc
    13681 atccatacca gcaagataat gaacgggatg gaacggaaaa tgttcacaat cgcagaaacg
    13741 gtacgataca gcttcgcgtt agcaataatt tgccccggac gcgtgacata aagcagaacg
    13801 ccaaccggca gaccaatcac aaacccaaaa aatccggata cgaaggtcat tgccagcgtt
    13861 tcccatacgc cacgaaccag cagccacatc atcggctcag acataaccca gtacctctac
    13921 ttttacatgg tgttcctgca gccaggcaat ggcggcttgc gtatcttgtt gtgtgccgtg
    13981 catttcagtc agcatgatgc cgaatttaac gccaccggcg tagtccatct gcgcgctaat
    14041 aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa agcagtgggg catcgaccga
    14101 ttgaccggta aactccagac gcagcatcgg cacgcagtca gtaaatggct ccgtttgcag
    14161 acgttcctgg taatcttccg ggatatccag atgcagggtc gactgaataa acttctgcgc
    14221 cagcggcgtt ttcggatgcg agaacacttc acttaccgtg tcctgctcga tcagttcgcc
    14281 attgctgatg accgccacgc aatcacaaat acgcttcaca acgtccattt cgtgggtgat
    14341 caacagaatc gtcaaaccca gacggcggtt aatgtctttc agcagttcga gaatagaacg
    14401 tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac agcaatactt tgggattgct
    14461 ggctaatgca cgggcaatcg ccacacgttg tttctgccca ccggaaagat tcgacgggta
    14521 gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat
    14581 ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc acgttgccaa aaacagtacg
    14641 cgaagagagc aggttaaaat gctggaaaat cataccaatc tggcggcgag ctttggtcaa
    14701 ctcggattct gacagcgtgg tcaattcctg gccatcgacc agcacgctac cctcggttgg
    14761 gcgctccagc aagtttacac aacgtataag cgtactctta cccgcgcctg aggcaccgat
    14821 aacgccataa atttgtccag ctggcacatg caggctgacg ttgttcaacg cctggatggt
    14881 gcgggtgccc tggtggaaca ctttggtgat attcgaaagt tttatcattg attatttatt
    14941 atcgtcatta agttagtcgt agcatctcga atgcctgaaa cgggcaacgc cgtcaatgaa
    15001 atggatgtta aggcatccag acgtctaaat caatcaggtt tatgcgaaga gcactttctt
    15061 gcaggtcgaa acatgcgata ctagcgtccc atgccttatt aaggagctat aaaaggtggc
    15121 gaagagcgta cccgcaattt ttcttgaccg tgatggcacc attaatgtcg atcacggcta
    15181 tgtccatgag atcgacaact ttgaatttat cgacggtgtt attgacgcca tgcgcgagct
    15241 aaaaaaaatg ggctttgcgc tggtggtagt aaccaaccag tctggcattg ctcgcggtaa
    15301 atttaccgaa gcacagtttg aaacgctgac cgagtggatg gactggtcgc tggcggaccg
    15361 agatgtcgat ctggatggta tctattattg cccgcatcat ccgcagggga gtgttgaaga
    15421 gtttcgccag gtctgcgatt gccgcaaacc acatccgggg atgcttttgt cagcacgcga
    15481 ttatttgcat attgatatgg ccgcttctta tatggtgggc gataaattag aagatatgca
    15541 ggcagcggtt gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta aacctattac
    15601 acctgaagca gaaaacgcgg cagattgggt gttaaatagc ctggcagacc tgccgcaagc
    15661 gataaaaaag cagcaaaaac cggcgcaatg attaaaagat gaacggttga aataaaaatg
    15721 catttttccg cttgtcttcc tgagccgact ccctataatg cgcctccatc gacacggcgg
    15781 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaaatcc tgaaattcag
    15841 ggttgactct gaaataggaa agtgtgatat acgccacctc gcgacagtga gctgaaagct
    15901 gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgggcactc gaagatacgg
    15961 attcttaacg tcgcaag
//
LOCUS       DAJTMX010000092        15930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTMX010000092 DAJTMX010000000
VERSION     DAJTMX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.092"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO145_002881"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO145_002881"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5467939.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO145_002882"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO145_002882"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5467940.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO145_002883"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO145_002883"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5467941.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO145_002884"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO145_002884"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5467942.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO145_002885"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO145_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO145_002886"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO145_002886"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5467943.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO145_002887"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO145_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO145_002888"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO145_002888"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5467944.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO145_002889"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO145_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5467945.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="OO145_002890"
                     /pseudo
     CDS             complement(8973..10211)
                     /locus_tag="OO145_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="OO145_002891"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="OO145_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5467946.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO145_002892"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO145_002892"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5467947.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO145_002893"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO145_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5467948.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="OO145_002894"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="OO145_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5467949.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="OO145_002895"
     CDS             14682..15584
                     /locus_tag="OO145_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5467950.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15930
                     /locus_tag="OO145_002896"
                     /pseudo
     CDS             15731..15930
                     /locus_tag="OO145_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3955 a         4116 c         3855 g         4004 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtcaca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagaaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc
//
LOCUS       DAJTMX010000093        15882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTMX010000093 DAJTMX010000000
VERSION     DAJTMX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.093"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1800)
                     /gene="malQ"
                     /locus_tag="OO145_002897"
     CDS             complement(<1..1800)
                     /gene="malQ"
                     /locus_tag="OO145_002897"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5467951.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCL"
     gene            complement(1810..4203)
                     /gene="malP"
                     /locus_tag="OO145_002898"
     CDS             complement(1810..4203)
                     /gene="malP"
                     /locus_tag="OO145_002898"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5467952.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            4815..7570
                     /gene="malT"
                     /locus_tag="OO145_002899"
                     /pseudo
     CDS             4815..7570
                     /gene="malT"
                     /locus_tag="OO145_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(7613..8629)
                     /gene="rtcA"
                     /locus_tag="OO145_002900"
     CDS             complement(7613..8629)
                     /gene="rtcA"
                     /locus_tag="OO145_002900"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5467953.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(8633..9859)
                     /gene="rtcB"
                     /locus_tag="OO145_002901"
     CDS             complement(8633..9859)
                     /gene="rtcB"
                     /locus_tag="OO145_002901"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5467954.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            10040..11638
                     /gene="rtcR"
                     /locus_tag="OO145_002902"
     CDS             10040..11638
                     /gene="rtcR"
                     /locus_tag="OO145_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5467955.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(11620..12378)
                     /locus_tag="OO145_002903"
     CDS             complement(11620..12378)
                     /locus_tag="OO145_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5467956.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(12395..13225)
                     /gene="glpG"
                     /locus_tag="OO145_002904"
     CDS             complement(12395..13225)
                     /gene="glpG"
                     /locus_tag="OO145_002904"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5467957.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(13270..13596)
                     /gene="glpE"
                     /locus_tag="OO145_002905"
     CDS             complement(13270..13596)
                     /gene="glpE"
                     /locus_tag="OO145_002905"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5467958.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            13786..15291
                     /gene="glpD"
                     /locus_tag="OO145_002906"
     CDS             13786..15291
                     /gene="glpD"
                     /locus_tag="OO145_002906"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5467959.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(15560..15790)
                     /locus_tag="OO145_002907"
                     /pseudo
     CDS             complement(15560..15790)
                     /locus_tag="OO145_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         3780 a         4404 c         3997 g         3701 t
ORIGIN      
        1 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
       61 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
      121 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
      181 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
      241 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
      301 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
      361 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
      421 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
      481 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
      541 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
      601 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
      661 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
      721 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
      781 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
      841 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
      901 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
      961 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     1021 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     1081 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     1141 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     1201 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     1261 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     1321 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     1381 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     1441 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     1501 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     1561 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     1621 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     1681 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     1741 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     1801 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     1861 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     1921 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     1981 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     2041 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     2101 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     2161 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     2221 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     2281 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     2341 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     2401 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     2461 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     2521 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     2581 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     2641 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     2701 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     2761 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     2821 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     2881 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     2941 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     3001 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     3061 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     3121 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     3181 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     3241 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     3301 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     3361 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     3421 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     3481 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     3541 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     3601 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     3661 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     3721 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     3781 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     3841 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     3901 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     3961 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     4021 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     4081 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     4141 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     4201 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     4261 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     4321 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     4381 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     4441 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     4501 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     4561 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     4621 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     4681 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     4741 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     4801 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     4861 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     4921 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     4981 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     5041 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     5101 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
     5161 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
     5221 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
     5281 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
     5341 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
     5401 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
     5461 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
     5521 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
     5581 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
     5641 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
     5701 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
     5761 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
     5821 tggcggcgga gctgccagtg ggaactggcg gcggagctgc cagtgggaac tggcggcgga
     5881 gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat ttcccagcga
     5941 agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa
     6001 tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc
     6061 cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg cgtggctaat
     6121 gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg aacatgaaat
     6181 caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg cccaggtggc
     6241 gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac tggaagagct
     6301 gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca
     6361 ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac agatggcacg
     6421 ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg aaattctgtt
     6481 tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc agctgatcaa
     6541 cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc gagcacaact
     6601 gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt
     6661 cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc
     6721 gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg aaaacctgct
     6781 ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta
     6841 ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc
     6901 ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat
     6961 cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg aaaatgcccg
     7021 cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc aactgtactg
     7081 gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa
     7141 tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg
     7201 ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc agcgtattct
     7261 gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga
     7321 acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt tgacccaacg
     7381 tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa ttgcgggcga
     7441 actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg
     7501 cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta
     7561 tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta catcattcaa
     7621 tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc aagaaacgct
     7681 ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta aattctcccg
     7741 cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc accgccgctg
     7801 ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc tcggcactga
     7861 cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg acttccagcg
     7921 agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt tcatgcaggg
     7981 aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc acaccagcta
     8041 atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgctaa
     8101 acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga taaaaaccgt
     8161 gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccagcaccc
     8221 ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc acttcaacac
     8281 gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacaac
     8341 ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca ggccggaaga
     8401 ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt tccgcagccg
     8461 cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg gctcgaatgc
     8521 cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag cgcaggatct
     8581 gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt
     8641 ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt tgtgccgcca
     8701 tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac
     8761 ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt tttttcgctt
     8821 tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc
     8881 ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt ccatattgac
     8941 cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca aagagctgtt
     9001 ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc
     9061 tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc atcgcatcgc
     9121 ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca tcaaagtatt
     9181 ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt tggtcctgca
     9241 tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac
     9301 cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca ataaagtggt
     9361 tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa cgcggatatt
     9421 tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg acgttaaaag
     9481 gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc ccgtgtggta
     9541 cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct gccgccgtta
     9601 acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc gccggaataa
     9661 tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg tgtacatcag
     9721 gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac
     9781 gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg
     9841 tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc tgataattgc
     9901 aaccgtcgtg ccagaaaatt aaataagcag attaacttat tgtttctaaa aagattatat
     9961 ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg aaatatcgcc
    10021 ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg gtaccgtgct
    10081 ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac tctgtatata
    10141 ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc gctcgcgctc
    10201 gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag aagtggtggg
    10261 cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct gcctgcatga
    10321 tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc acatcaccac
    10381 cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt atctacccgc
    10441 ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc caggtgaagt
    10501 gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct ttgccgagga
    10561 acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc cccacttcaa
    10621 ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga ttctgcttaa
    10681 cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt taaaacaggc
    10741 acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc gcggcgatac
    10801 cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc gggaatctcg
    10861 tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga ttggcgaact
    10921 gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct tttacccgtt
    10981 tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg tgcgcgattt
    11041 gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga tcaatctctg
    11101 gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga acctggatta
    11161 tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca ccgaagcgcg
    11221 gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta actttcgcga
    11281 actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca tcactctgga
    11341 cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc gcccctccgc
    11401 gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc gtatgcaact
    11461 ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg gacgacagct
    11521 ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc tacgcaaata
    11581 cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct ccagttgaat
    11641 atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg tgtagacggc
    11701 atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt ttgaatggtc
    11761 aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac gaacttcgtg
    11821 gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca gaatgccgaa
    11881 atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc cgccatcgcg
    11941 gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca tcaacgtgtt
    12001 agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca gcgcgtgcgc
    12061 caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg ggatttgctc
    12121 cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac gatcgtgcca
    12181 cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca ggatcaggtt
    12241 ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg agaagtgctc
    12301 caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa taccgttgtg
    12361 acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc gcattgagcg
    12421 aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct ccgttcgcca
    12481 tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca aagataatta
    12541 acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc cagacgtagc
    12601 ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg aatttttgct
    12661 gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta ccgctaccga
    12721 ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag aggatatgca
    12781 tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat ttcagtgttg
    12841 gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt tgcatggcaa
    12901 taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg cgttcacgca
    12961 aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg cctgccagcc
    13021 agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc agctccgccc
    13081 gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta tgttgttgaa
    13141 tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc acgcgggggt
    13201 tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa aaggggacag
    13261 tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc aggcttcaaa
    13321 gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact gcgccgcccc
    13381 tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt cgttatcacg
    13441 cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat gccccatagc
    13501 gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca acttctggtg
    13561 cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct ttacaaacaa
    13621 gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat aatgttatac
    13681 atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc taatatgttc
    13741 gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga aaccaaagat
    13801 ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc cgctggacgc
    13861 ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc ttccgccagt
    13921 tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg cctggtcagc
    13981 gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc cttcccgatg
    14041 cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg cattggtctg
    14101 tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg tttgcgtttt
    14161 ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc tgactgttgg
    14221 gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa aggcggcgaa
    14281 gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg gattgtggaa
    14341 gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct ggttaacgcc
    14401 accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc gccttatggc
    14461 attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca aaagcaggcc
    14521 tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat ggacgagttt
    14581 tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt gaagattgaa
    14641 gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa gcagctaagc
    14701 cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga tgagtccgac
    14761 tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa cggcaaagca
    14821 ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc agaacatgcg
    14881 ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa agagagcgtg
    14941 ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct gcgccgccgt
    15001 tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg cagcaacagc
    15061 gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt cggtcatgag
    15121 ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg caccgacgat
    15181 gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca atctcgtgtg
    15241 agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta aattaacgta
    15301 aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa aactgcctga
    15361 tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc atggtcttgt
    15421 aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt gtcaaaaatc
    15481 taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac ccaacgtaac
    15541 aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg cccagcgtca
    15601 gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg caaacctcag
    15661 gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat tgcgtcagta
    15721 ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta ttttgcatta
    15781 ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg ccccttctgg
    15841 acacttttcc gaaatgatgg cgaaaaaaac gggacccttt gg
//
LOCUS       DAJTMX010000094        15524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTMX010000094 DAJTMX010000000
VERSION     DAJTMX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.094"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..74)
                     /locus_tag="OO145_002908"
     CDS             complement(<1..74)
                     /locus_tag="OO145_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5467960.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO145_002909"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO145_002909"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5467961.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="OO145_002910"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="OO145_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5467962.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO145_002911"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO145_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5467963.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="OO145_002912"
     CDS             2704..2940
                     /locus_tag="OO145_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5467964.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="OO145_002913"
     CDS             2943..3800
                     /locus_tag="OO145_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5467965.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="OO145_002914"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="OO145_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="OO145_002915"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="OO145_002915"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5467966.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="OO145_002916"
     CDS             complement(6415..7311)
                     /locus_tag="OO145_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5467967.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="OO145_002917"
     CDS             complement(7360..7599)
                     /locus_tag="OO145_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5467968.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="OO145_002918"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="OO145_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5467969.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="OO145_002919"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="OO145_002919"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5467970.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="OO145_002920"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="OO145_002920"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5467971.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="OO145_002921"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="OO145_002921"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5467972.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..12880
                     /locus_tag="OO145_002922"
     CDS             10766..12880
                     /locus_tag="OO145_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5467973.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12890..14182
                     /locus_tag="OO145_002923"
     CDS             12890..14182
                     /locus_tag="OO145_002923"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5467974.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15132..15224
                     /locus_tag="OO145_002924"
     CDS             15132..15224
                     /locus_tag="OO145_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5467975.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15268..>15524)
                     /locus_tag="OO145_002925"
     CDS             complement(15268..>15524)
                     /locus_tag="OO145_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5467976.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3560 a         4109 c         4079 g         3776 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccac
//
LOCUS       DAJTMX010000095        15411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTMX010000095 DAJTMX010000000
VERSION     DAJTMX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.095"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1177)
                     /locus_tag="OO145_002926"
     CDS             complement(<1..1177)
                     /locus_tag="OO145_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5467977.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="OO145_002927"
     tRNA            complement(1375..1459)
                     /locus_tag="OO145_002927"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="OO145_002928"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="OO145_002928"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5467978.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4305
                     /locus_tag="OO145_002929"
     CDS             2803..4305
                     /locus_tag="OO145_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5467979.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO145_002930"
     CDS             complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO145_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5467980.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO145_002931"
     CDS             complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO145_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5467981.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6873..8384
                     /gene="pepA"
                     /locus_tag="OO145_002932"
     CDS             6873..8384
                     /gene="pepA"
                     /locus_tag="OO145_002932"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5467982.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8518..8961
                     /gene="holC"
                     /locus_tag="OO145_002933"
     CDS             8518..8961
                     /gene="holC"
                     /locus_tag="OO145_002933"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5467983.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8961..11816
                     /gene="valS"
                     /locus_tag="OO145_002934"
     CDS             8961..11816
                     /gene="valS"
                     /locus_tag="OO145_002934"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5467984.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11870..13066)
                     /locus_tag="OO145_002935"
     CDS             complement(11870..13066)
                     /locus_tag="OO145_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5467985.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13259..13762
                     /locus_tag="OO145_002936"
     CDS             13259..13762
                     /locus_tag="OO145_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5467986.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="OO145_002937"
     CDS             complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="OO145_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5467987.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14386..15399
                     /gene="argF"
                     /locus_tag="OO145_002938"
     CDS             14386..15399
                     /gene="argF"
                     /locus_tag="OO145_002938"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5467988.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3977 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggca ccccgccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggcccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 agtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagatcaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgttcgg ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg gggagaaaaa gataagtcag acgtgatctg
     4321 tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga
     4381 ctttgtcaac aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta
     4441 acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg atgatcggcg
     4501 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4561 cgaagctgat acctgtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4621 taaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4681 acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     4741 tgtgcaaacc actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     4801 gcgtgaggtc ggttttccag gtgccgctca ccgtctgcga accggtaatc tgtttcggat
     4861 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     4921 tcgcggtagc ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     4981 tgatgccacc taacacctcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5041 ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5101 ggtagttacg cgccatctgt tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5161 gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata cgggtaaaac
     5221 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5281 ccccaagaag agccgccatc gggaagaaga tctgcacatc tttcggcacg ctcagcaagg
     5341 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     5401 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5461 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5521 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5581 cgccaaagcc agataaatca ggttaacggt ccacatccac agtgtcgggt ccagcttacc
     5641 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     5701 ggctggcagc atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac
     5761 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     5821 agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg
     5881 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     5941 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6001 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6061 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6121 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6181 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6241 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6301 tgccgctttc accagaaccg ctttgctcaa gccgcaggca tgcattaccg taatttcact
     6361 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6421 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6481 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6541 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6601 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6661 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     6721 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     6781 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     6841 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     6901 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     6961 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgccggg
     7021 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7081 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7141 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcgatg gaagcggtct
     7201 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7261 tcgagacagc aaaagagacg ctctacagtt tcgatcagtt gaaaacgaat aagagcgaac
     7321 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7381 gtgagcgcgc gatccagcac ggtcaggcga ttgccgccgg gataaaagca gcgaaagatc
     7441 tcggtaatat gccgccgaat atctgtaacg ccgcttatct cgcctctcag gcgcgccagc
     7501 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     7561 agctgggaat gcactcttat ctggcggtcg gtcagggttc acaaaacgaa tcgctgatgt
     7621 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7681 aaggtttaac cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga
     7741 tgaagtacga tatgtgcggc gcggcggcag tttacggcgt gatgcgtatg gtcgcggagc
     7801 tgcaactgcc gattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc
     7861 gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     7921 acaccgatgc cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg
     7981 agccggaagc agtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8041 atcatattac tggtctgatg acgaaccata atccgctggc ccatgaactg attgccgcgt
     8101 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8161 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8221 caggttgctt cctgtcacgc tttacccgta agtataactg ggcgcacctg gatattgcag
     8281 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8341 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct
     8401 caccctaacc ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct
     8461 catctttcag ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg
     8521 aaaaacgcaa cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt
     8581 gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtactcatc
     8641 gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca
     8701 gaaagctttg ttccgcataa tttagcggga gaaggaccgc gcggcagtgc gccggtggag
     8761 atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca
     8821 agctttgcag attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat
     8881 tctctgaaac aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg
     8941 aatacggcaa cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc
     9001 tttacgagca ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa
     9061 gtttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct
     9121 tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc
     9181 tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
     9241 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa
     9301 tctgggaatg gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca
     9361 actccgtcga ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga
     9421 aagaagtttt cgttcgtctg tataaagaag acctgattta ccgtggcaag cgcctggtaa
     9481 actgggaccc gaaactgcgt accgctattt ctgacctgga agtggaaaac cgcgaatcga
     9541 aaggttcgat gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta
     9601 aagattatct ggtggttgcg accactcgcc cggaaaccct gctgggcgat accggtgtag
     9661 ctgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc
     9721 tggttaaccg tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg
     9781 gctgcgtgaa gatcacaccg gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg
     9841 ccctgccgat gatcaacatc ctgacctttg acggtgatat ccgtgaaagc gcccaggtgt
     9901 tcgataccaa aggtaacgaa tctgacgttt attccagcga gatcccggca gagttccaga
     9961 aactcgagcg ttttgctgca cgtaaagcag tcgttgctgc ggttgatgca ctcggcctgc
    10021 tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtggtta
    10081 tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcag
    10141 ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact
    10201 tctcctggat gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc
    10261 gtatcccggc atggtatgac gaagcgggta atgtttatgt tggccgcaac gaagaagaag
    10321 tgcgcaagga aaataacctc ggtgccaacg ttgccctgcg tcaggacgaa gacgttctcg
    10381 acacctggtt ctcttctgca ctgtggacct tctctaccct tggctggccg gaaaataccg
    10441 acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcatcttct
    10501 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac
    10561 cgcaggtgcc gttccacact gtttacatga ccggtctgat tcgtgacgac gaaggtcaga
    10621 agatgtccaa atccaagggt aacgttattg acccgctgga tatggttgac ggtatttcgc
    10681 tgccagaact gctggaaaaa cgcaccggca atatgatgca gccgcagctg gcggacaaaa
    10741 tccgtaagcg caccgagaaa cagttcccga acggcattga gccgcacggc accgatgccc
    10801 tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc
    10861 gtctggaagg ctaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
    10921 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgcta tcgctggcgg
    10981 accgctggat tctggcggag ttcaaccaga ctatcaaagc gtaccgcgaa gcgctggaca
    11041 gcttccgctt cgatatcgcc gcaggcattt tgtatgagtt cacctggaac cagttctgcg
    11101 actggtatct ggagctgacc aagccggtaa tgaacggtgg aacagaagcc gaactgcgcg
    11161 gtactcgcca tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca
    11221 ttccgttcat caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg
    11281 acaccatcat gctgcagccg ttcccgcagt atgatgcatc tcaggttgat gaagccgcac
    11341 tggccgacac cgagtggctg aaacaggcga tcgttgctgt acgtaacatc cgtgcagaaa
    11401 tgaacatcgc gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag
    11461 aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct ggcgcgtctg gaaagcatca
    11521 ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac caagatcatc gacggcgcag
    11581 agctgctgat cccgatggct ggcctcatca acaaagaaga tgagctggcg cgcctggcga
    11641 aagaagtggc gaagatcgaa ggtgaaatca gccgcatcga gaacaagctg gcaaatgaag
    11701 gctttgtcgc ccgcgcaccg gaagcggtta tcgcgaaaga gcgtgagaag ctggaaggtt
    11761 atgcagaagc gaaagccaag ctgattgaac agcaggctgt tatcgccgca ctgtaatcaa
    11821 cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    11881 gtaaagaagg cataactccc cgagaaatac tggctaagaa gcctttatct ggttgttctt
    11941 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    12001 agtcaatcat ccatattttc agcagtgggt aagccagacc acccgtaatc ccggatatca
    12061 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    12121 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    12181 aactggtgct aacagcaatc ccaaaataat aaattaactg cgcgattatc attttacgtt
    12241 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcataccca gaactatcat
    12301 actcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    12361 ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc accttgaaac
    12421 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    12481 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    12541 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    12601 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat
    12661 taaaactaaa tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg
    12721 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagcggca
    12781 tatctgctga cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa
    12841 caaaaacatt cccaccggta attccataag aaaatcgttg tccgttaact tccatattag
    12901 cataaagata acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa
    12961 tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa
    13021 caaaagaatg ggacggaaca tccatttcat taataacttg agccataaaa atcccctgaa
    13081 ttaaaaaatc cattgatata atttatagat acagacagta aaatgttctg ccacgttaca
    13141 atactcttat cagtaatagg ctggcaaaaa ctttattgct ttataagacg cttgcgcacg
    13201 taagaagaga gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat
    13261 gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctgcagg ataatcctgc
    13321 tatcgccagc gtcatccgtc aggtttacgc agagtatggt cttaccgccg ataaaggcta
    13381 caccgttgcc gatccgaatc tcgacgaact gtatcaagtg tacagccagc ctggccatgc
    13441 ttattgggtg gttgaataca atggcgaagt ggtcggcggc ggcgggattg cgccattaac
    13501 aggtagtgaa gcggacattt gcgaactgca gaaaatgtac tttcttcccg ctattcgcgg
    13561 taaagggcta gcgaaaaaac tggccttaat ggcgatggag caggcacgag agatgggctt
    13621 caaacgctgc tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc tttatgagca
    13681 tttgggcttt gagcatatcg actatgcgct tggctgcacg ggccatgtcg attgtgaagt
    13741 gcggatgcta cgcgaactct aaaaaaatgc ccgctagtat gtgctatcgg gcattttaat
    13801 caattaatta gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccat
    13861 cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct
    13921 ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact
    13981 cgctgaggat gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat
    14041 cggtcacttc gtaaccgagt ttaaacgctt caactgccgc tttttccaga gtttccagat
    14101 cgtctgcgga aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca
    14161 gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg
    14221 ccatggctca ttcctcttaa tgtcctgccg atactcttat tgtcacacac cactgacatt
    14281 gcctccacct ttgtgacaaa gatttatgct ttagacttgc aaatgaataa taatacacat
    14341 aaattgagtt ttaattcatt gaggcgttag ccacaggagg gatctatgtc cgggttttat
    14401 cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag cctgctgcag
    14461 ttagccgcga agctgaaagc tgataagaaa agcggtaaag aagaagccaa actcactggt
    14521 aaaaacatcg cgctcatctt cgaaaaagac tcgactcgta cccgatgctc tttcgaagtt
    14581 gccgcatatg accagggggc tcgcgttact tatcttggcc caagcggtag tcagattggt
    14641 cataaagagt cgattaaaga cactgcccgc gtgcttggtc gcatgtatga cggtattcag
    14701 tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta
    14761 tggaacggcc tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg
    14821 caggagcatt tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca
    14881 cgtaacaaca tgggcaattc gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt
    14941 ctggtcgcgc cacaggcatg ctggccagaa gctgcgctgg ttacggaatg ccgcgccctg
    15001 gcacaacaaa atggcgggaa tattacgctg acggaagatg tagctaaagg agttgaaggt
    15061 gctgacttta tctataccga tgtatgggtg tcgatgggtg aagcaaaaga aaaatgggct
    15121 gaacgcattg cattactgcg tgattatcag gtgaacagca agatgatgca gttgaccggt
    15181 aacccggagg tcaaattcct ccattgcctg cccgcgtttc atgacgacca aacgacgctt
    15241 ggcaaaaaaa tggcggaaga gtttggccta catggcggaa tggaagtgac tgatgaggtc
    15301 ttcgaatctg ccgccagcat tgtatttgat caggcggaaa accgcatgca taccatcaaa
    15361 gcggtgatgg tcgcgacgct cagtaaatta aataattaat atctctataa a
//
LOCUS       DAJTMX010000096        15343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTMX010000096 DAJTMX010000000
VERSION     DAJTMX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.096"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO145_002939"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO145_002939"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5467989.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO145_002940"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO145_002940"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5467990.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO145_002941"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO145_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5467991.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO145_002942"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO145_002942"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5467992.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO145_002943"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO145_002943"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5467993.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO145_002944"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO145_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5467994.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO145_002945"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO145_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5467995.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO145_002946"
     CDS             complement(8064..9206)
                     /locus_tag="OO145_002946"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5467996.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO145_002947"
     CDS             complement(9215..10228)
                     /locus_tag="OO145_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5467997.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO145_002948"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO145_002948"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5467998.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO145_002949"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO145_002949"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5467999.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO145_002950"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO145_002950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5468000.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAE
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO145_002951"
     CDS             complement(12826..13950)
                     /locus_tag="OO145_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5468001.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO145_002952"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO145_002952"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5468002.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4381 a         3444 c         3144 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc aatcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt tttccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DAJTMX010000097        15279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTMX010000097 DAJTMX010000000
VERSION     DAJTMX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.097"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..548)
                     /locus_tag="OO145_002953"
     CDS             complement(<1..548)
                     /locus_tag="OO145_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5468003.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPT"
     gene            767..1687
                     /gene="ldtB"
                     /locus_tag="OO145_002954"
     CDS             767..1687
                     /gene="ldtB"
                     /locus_tag="OO145_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5468004.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(1745..2863)
                     /locus_tag="OO145_002955"
     CDS             complement(1745..2863)
                     /locus_tag="OO145_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5468005.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(2860..3327)
                     /gene="mntR"
                     /locus_tag="OO145_002956"
     CDS             complement(2860..3327)
                     /gene="mntR"
                     /locus_tag="OO145_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5468006.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            3513..3641
                     /gene="mntS"
                     /locus_tag="OO145_002957"
     CDS             3513..3641
                     /gene="mntS"
                     /locus_tag="OO145_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5468007.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            3913..5496
                     /gene="opgE"
                     /locus_tag="OO145_002958"
     CDS             3913..5496
                     /gene="opgE"
                     /locus_tag="OO145_002958"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5468008.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(5544..6059)
                     /gene="ompX"
                     /locus_tag="OO145_002959"
     CDS             complement(5544..6059)
                     /gene="ompX"
                     /locus_tag="OO145_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5468009.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(6112..6177)
                     /locus_tag="OO145_002960"
     CDS             complement(6112..6177)
                     /locus_tag="OO145_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468010.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            6412..7299
                     /gene="rhtA"
                     /locus_tag="OO145_002961"
     CDS             6412..7299
                     /gene="rhtA"
                     /locus_tag="OO145_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5468011.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            7598..8101
                     /gene="dps"
                     /locus_tag="OO145_002962"
                     /gene_synonym="pexB"
     CDS             7598..8101
                     /gene="dps"
                     /locus_tag="OO145_002962"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5468012.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            8505..9251
                     /gene="glnH"
                     /locus_tag="OO145_002963"
     CDS             8505..9251
                     /gene="glnH"
                     /locus_tag="OO145_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5468013.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            9390..10049
                     /gene="glnP"
                     /locus_tag="OO145_002964"
     CDS             9390..10049
                     /gene="glnP"
                     /locus_tag="OO145_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5468014.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            10046..10768
                     /gene="glnQ"
                     /locus_tag="OO145_002965"
     CDS             10046..10768
                     /gene="glnQ"
                     /locus_tag="OO145_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5468015.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            11029..13253
                     /gene="ybiO"
                     /locus_tag="OO145_002966"
                     /pseudo
     CDS             11029..13253
                     /gene="ybiO"
                     /locus_tag="OO145_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(13250..14176)
                     /gene="rlmF"
                     /locus_tag="OO145_002967"
     CDS             complement(13250..14176)
                     /gene="rlmF"
                     /locus_tag="OO145_002967"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5468016.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            14452..14712
                     /locus_tag="OO145_002968"
     CDS             14452..14712
                     /locus_tag="OO145_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5468017.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            14977..>15279
                     /locus_tag="OO145_002969"
     CDS             14977..>15279
                     /locus_tag="OO145_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5468018.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         3791 a         3965 c         3723 g         3800 t
ORIGIN      
        1 gtcggttcgt cgagcaggag aatatccggc tcagcaaaca gcgcctgcgc cagaagcaca
       61 cgcagcttcc agccaggagc aacttcactc atcgggccgt agtgctgttc cactggaatt
      121 cccacgccaa gcaacagttc accggcgcga gcttccgcag agtaaccgtc catttcgccg
      181 tatttcactt ccagatcggc cactttatag ccgtcttctt cactcatttc cggcaaagca
      241 tagatgcggt cgcgctcctg cttcacttcc cacaactctt tatgccccat gatcaccgta
      301 tccagcacag tgaactcttc aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg
      361 ttgggatcga gggaaacgtt acccagcgtc ggctcaaggt cgccgccgag aatcttcata
      421 aaggtggatt taccactacc gttcgcgcca atcaggccgt aacggttgcc gccgccgaat
      481 ttgacggaaa tgttttcaaa caacggctta ctgccgaact gcatggtgac gttactggaa
      541 actaacacag gcgtatcctg aaaagagata tgacaaaccg cgcattatgc cataagtctg
      601 aaagaagatc ctgctcctcg cttaaggtct acactcaagc caaaggcaaa aaatgtgatt
      661 tcgtacacat ctgatttcac tgtgagctgg aatgaactta taatgcgctt ccaatactct
      721 aatattctca acccaatggc ctgccaggca caaaatctcg cttaacatga atatgaaatt
      781 gaaaacatta ttcgcagcgg ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt
      841 aacttatcct ctgccaaccg acgggagtcg cctggttggt cagaatcagg tgatcaccat
      901 tcctgaaggt aacactcagc cgctggagta ttttgccgcg gagtaccaga tggggctttc
      961 caatatgatg gaagcgaacc cgggtgtgga taccttcctg ccgaaaggcg gtactgtact
     1021 gaacattccg cagcagctga tcctgccgga taccgttcat gaaggcatcg tcattaacag
     1081 tgcagagatg cgtctgtatt actatccgaa agggaccaac accgttatcg tgctgccgat
     1141 cggcattggt cagttaggca aagatacgcc tatcaactgg accaccaaag ttgagcataa
     1201 gaaagcaggc ccgacctgga cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg
     1261 cgaaccgcta ccggctgtcg ttccggcagg tccggataac ccgatgggtc tgtatgcact
     1321 ctacatcggt cgcctgtatg ctatccatgg caccaacgcc aacttcggta tcggcctgcg
     1381 tgtaagtcat ggttgtgtgc gtctgcgtaa cgaagacatc aaattcctgt tcgagaaagt
     1441 accggtcggt acccgcgtac agtttattga tgagccggta aaagcgacca ccgagccaga
     1501 cggcagccgt tatattgaag tccataaccc gctgtctacc accgaagccc agtttgaagg
     1561 tcaggaaatc gtgccaatta ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga
     1621 tgttgaccag gttgttcttg atgaagcgat caaaaatcgt tccgggatgc cggttcgcct
     1681 gaattaatcg tcaaaacgta aaacaaaggc ggactcataa tccgcctttt ttatttgcca
     1741 gaccttacca ggccgggatc ataactaaca aaacgtatcc caccagcgcc gcccacaaca
     1801 gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc gccatacgta
     1861 gcgcaatcaa atttgccagc gatcctggta ataaaccaaa gccccccacg tttaccgccc
     1921 atgccagtaa taaagacgga ggtacataat tgagcagcaa aatggtactc ggcacgttac
     1981 tgatcacctg cgataaaccg actgccgtta accataaccc gggttcagat aaatgactca
     2041 cgttacccaa cacgccttgc aacgctggaa gctgggtcag taaatggacg tcgataaaca
     2101 tcgccataaa caccagcagc aacgtccagt ccacactcaa caccacgcga cgcgccagca
     2161 gcacaaagcc cgccgccaca atcaccagtc cccacagctc ttgtttgaac tccagcgccg
     2221 tcagaaagac gatatacagc cccagacaat tccacaccag ccgcggtttc cactccggtg
     2281 tttgcacccc cgtatggtat tgcagcgcct ttccagggaa acaacaccag cacaacagca
     2341 ggagtgtcag catcattgcg ccagccatcg gtgccatttg ggcaataaat ccggcaaacg
     2401 aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc gtcagtagcg
     2461 aaccagcgtt gactgccagc gcctcaaaaa taatcagccg attaaccggg atctcacaca
     2521 gtctttttag cgtgatagtc agcggaacaa caataaacag cgcgacatcg ttggtcagaa
     2581 aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc tccgtagcaa
     2641 agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc acacctttgg
     2701 tcagcagcat caggccgctt aaggtgatga tggtgtgcca gtcgatagca gcaggccagg
     2761 atttcggtgc aaagggcacg aaaaagctta atccgatacc aacaagaatt aataactgaa
     2821 aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg
     2881 ggtaaacaaa cgaaaagcgt cgagcgtctc ttcactaaca tggtgctcca tgccttccgc
     2941 gtcgcgacgg gcgatttccg gactgacgcc caacaccaac aagaaatttt cgactatctg
     3001 atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa acacgccacg
     3061 ccaggggatc atttcaatca gccccatggt tgccagccgc ttaagcattt tagccaccgt
     3121 cggttgcgaa actcccagac gagcagccat gtccacctga cgagcttccc ccacttccct
     3181 gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat gcgcctctct
     3241 gacctggcgg aacccttcaa cgtgctcttc cacgttcact aactgcgtca ctttttttgc
     3301 tattggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg catacatagc
     3361 gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt
     3421 tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat catccctcaa
     3481 ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg
     3541 tgtttagtca ttctcccttt aaagtacggt taatgctgct ctctatgttg tgcgatatgg
     3601 tcaacaacaa accgcagcaa gataaacctt cagataaata gcggcgtcgc ggtacgccgc
     3661 ttcactcctg cttttatgca ggcataacgc gttttggtct gaaaaaaacc actttttgtc
     3721 ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga acggcggctc
     3781 gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc cctttcccct
     3841 cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg gcgtttgtta
     3901 cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt
     3961 agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg cttaggttac
     4021 cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct atggcgaaca
     4081 ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc ttgttatttc
     4141 ccttttgctc aggcctacgg cgcgcctaat ttcaatacat tgctggcatt gcactccacc
     4201 aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta cctggtcggc
     4261 ttatttattt ttgcgctcgg cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg
     4321 cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt ttttgttgct
     4381 cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg ctatccggta
     4441 tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga gcaagaacgg
     4501 atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa gccgaagtat
     4561 cagacctatg tggtagtgat cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc
     4621 ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt tgctgactac
     4681 attgctgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat
     4741 ggcaaaccac agtttcagga taactttgtc accctggcaa atcgcgcggg cttccagacc
     4801 tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc
     4861 aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg
     4921 aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg
     4981 cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgacag gacacaaggc
     5041 aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct atctctatac catgacgcaa
     5101 acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag cagcttctct
     5161 ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac
     5221 cttgcccatg atgataaata tcagcaaaat ttccaggtgc cttttatggt catttccagc
     5281 ggcgataaag cgcatcgggt gattaaagcc cgccgctcag ccaatgactt cttaggcttt
     5341 ttctcgcagt ggacgggaat taaagcgaag gaaataaata tcaaataccc gtttatatct
     5401 gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt ggattacaac
     5461 catctcggaa ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc
     5521 ggatttttat atcaccaaag tgattagaag cggtaaccaa caccggcaat ccaggtgcct
     5581 acgtcaacgc tacgaatacg gctctgctcg taagagaagt ccagagcaac gttttccatc
     5641 gggttgaact gcagaccagc accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag
     5701 gtcgggtatt cagtggtctg gaatttacca taacccacac ccactacacc gtagatgctt
     5761 gcccagtcgt taatgcggta agccggacca gcagtgatgc cgtagtactg gtttttgttg
     5821 tagtcaccag agcttgcagt acggcttttc tcggtgtaag tgaaagaacc gatcacaccc
     5881 agcgggctgt tgtcttcttc atagcggtat ttcaggttga aaccgcccat tttgttcatt
     5941 tggccctgag cgtcgctctg tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa
     6001 gtacctgcgg tgaaagccag aactgcggcc agtgctgaaa gacatgcaat ttttttcata
     6061 accacctcaa atgtgattca aataagtcct aagttttaaa tatatcaaaa attaatggga
     6121 aactcttcgc gatttgtgat gtctaacggg ccatttcatg taacagaacg tttccataca
     6181 ccgctatcca tctaaattta aatcactttt tcagagaact gcgtaagtat tacgcatgtt
     6241 ttccctgtca ttcatccaga ttattcctaa tcaccagact aatgattcca tcaatcctgg
     6301 cgcattttag tcaaaacggg ggaaaatttt ttcaacaaat gctcaaccag cattgggtat
     6361 atccagtaca ctccacgctt tacttaagtc tagatatttg tgggagaaag gatgcctggt
     6421 tcattacgta aaatgccggt ctggttacca atagtcatat tgctcgttgc catggcgtct
     6481 attcagggtg gagcctcgtt agctaagtca ctttttcctc tggtgagcgc accgggtgtc
     6541 actgcgctgc gtctggcatt aggaacgctg atcctcatcg cgttctttaa gccatggcga
     6601 ctgcgctttg ccaaagagca acggttaccg ctgttgtttt acggcgtttc gctgggtggg
     6661 atgaattatc ttttttatct ttctattcag acagtaccgc tgggtattgc ggtggcgctg
     6721 gagttcaccg gaccactggc ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc
     6781 tgggttgtgc tggcggttct tggtctgtgg ttcctgctac cgctggggca agacgtttcc
     6841 catgtcgatt taaccggctg tgcgctggca ctgggggccg gggcttgttg ggctatttac
     6901 attttaagtg ggcaacgcgc aggagcggaa catggccctg cgacggtagc aattggttcg
     6961 ttgattgcag cgttaatttt cgtgccaatt ggagcgcttc aggctggtga agcactctgg
     7021 cactggtcgg ttattccatt gggtctggct gtcgttattc tctcgaccgc tctgccttat
     7081 tcgctggaaa tgattgccct cacccgtttg ccaacacgga catttggtac gctgatgagc
     7141 atggaaccgg cgctggctgc cgtttccggg atgattttcc tcggagaaac actgacaccc
     7201 atacagctgc tggcgctcgg cgctatcatc gccgcttcaa tggggtctac gctgacagta
     7261 cgcaaagaga gcaaaataaa agaattagac attaattaaa tttacatttc tgtatggtta
     7321 tgcataacca tgcagaattt ctcgctactt ttcctctaca ccgtatttat atatcgaatt
     7381 atgcaaaagc atatttattc cgaaaattcc tggcgagcag ataaataaga attgttctta
     7441 tcaatatatc taactcattg aatctttatt agttttgttt ttcatgcttg ttaccactat
     7501 tagtgtgata ggaacagcca gaatagcgga acacatagcc ggtgctatac ttagtctcgt
     7561 taattactgg gacataacat caagaggata tgaaattatg agtaccgcta aattagttaa
     7621 atcaaaagcg accaatctgc tttatacccg caacgatgtc tccgacagcg agaaaaaagc
     7681 aacagtagag ttgctgaatc gccaggttat ccagtttatt gatctttctt tgattaccaa
     7741 acaagcgcac tggaacatgc gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg
     7801 cttccgcacc gcactgatcg atcatctgga taccatggca gaacgtgccg tgcagctggg
     7861 cggtgtagct ctggggacca ctcaagttat caacagcaaa accccgctga aaagttaccc
     7921 gctggacatc cacaacgttc aggatcacct gaaagaactg gctgaccgtt acgcaatcgt
     7981 cgctaatgac gtacgcaaag cgattggcga agcgaaagat gacgacaccg cagatatcct
     8041 gaccgccgcg tctcgcgacc tggataaatt cctgtggttt atcgagtcta acatcgaata
     8101 aatccatcgc tgatggtgca gaactttagt acccgataaa agcggcttcc tgacaggagg
     8161 ccgttttgtt ttgcagccca cctcaacgca cttatttagt gcatccatct gctatcccca
     8221 gctgattaag taaatttttt gtatccacat catcacacaa tcgttacata aagattgttt
     8281 tttcatcagg ttttacgcta aataatcact gtgttgagtg cacaatttta gcgcaccaga
     8341 ttggtgcccc agaatggtgc atcttcaggg tattgcccta taaatcgtgc atcacgtttt
     8401 tgccgcatct cgaaaaatca atgagttgca aaattggcac gattttttca tatatgtgaa
     8461 tgtcacgcag gggatcgtcc cgtggataga aaaaaggaaa tgctatgaag tctgtattaa
     8521 aagtttcact ggctgcactg accctggctt ttgcggtttc ttctcatgcc gcggataaaa
     8581 aattagttgt cgcgacggat accgccttcg ttccgtttga atttaaacag ggcgataaat
     8641 atgtgggctt tgacgttgat ctgtgggctg ccatcgctaa agagctgaag ctggattacg
     8701 aactgaagcc gatggatttc agtgggatca ttccggcact gcaaaccaaa aacgtcgatc
     8761 tggcgctggc gggcattacc atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg
     8821 gctactacaa aagcggcctg ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg
     8881 tgaaagatct cgacgggaaa gtggttgctg tgaagagcgg tactggctcc gttgattacg
     8941 cgaaagcaaa catcaaaact aaagatctgc gtcagttccc gaacatcgat aacgcctata
     9001 tggaactggg caccaaccgc gcagacgccg ttctgcacga tacgccaaac attctgtact
     9061 tcatcaaaac cgccggtaac ggtcagttca aagcggtagg tgactctctg gaagcgcagc
     9121 aatacggtat tgcgttcccg aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt
     9181 tgaaaaccct gcgcgagaac ggaacttaca acgaaatcta caaaaaatgg ttcagtactg
     9241 aaccgaaata ataacgctac acctgtaaag cgcactggca gttccctctc ccccctgggg
     9301 agaggattag ggtgaggggc gcaaacccgc tccggggcca ttaattaccc tgaatttgat
     9361 tatttacacc acggtaacag gaacaacata tgcagtttga ctggagtgcc atctggcctg
     9421 ccattccgct tctgattgaa ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg
     9481 ctggcggtct ggtaatcgga ttgctggcag gttttgcacg caccttcggt ggttggatag
     9541 ccaaccacgt cgcgctggtc tttattgaag tgatccgcgg cacacctatc gtcgtccagg
     9601 tgatgtttat ctatttcgcc ctgccgatgg cgtttaacga cttacgcatc gacccattta
     9661 ctgcggcggt ggtcaccatc atgatcaact ccggcgcgta cattgcggaa atcacgcgtg
     9721 gtgcggtgct gtctatccac aaaggttttc gtgaagcagg actggcgctc ggtctttcac
     9781 gttgggaaac cattcgctac gtcattttac cgctggcact gcgtcgtatg ctgccgccgc
     9841 tgggtaacca gtggatcatc agcattaaag acacctcgct gtttattgtt atcggcgtgg
     9901 cggaactgac ccgtcagggg caagaaatta ttgccggtaa cttccgcgcc cttgagatct
     9961 ggagcgccgt ggcggtgttc tatctgatta ttaccctggt gctgagcttt attctgcgtc
    10021 gtctggaaag aaggatgaaa atcctgtgat tgaatttaaa aacgtctcca agcactttgg
    10081 cccaacccag gtgctgcaca atatcgattt gaacattgcc cagggcgaag tcgtggtgat
    10141 tatcgggccg tccggttccg gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga
    10201 aatcacctcc ggcgatctga ttgtcgatgg cctgaaggtc aacgatccga aagttgacga
    10261 gcgcctgatt cgccaggaag caggtatggt gttccagcag ttttacctct tcccgcatct
    10321 gacggcgctg ggaaacgtca tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga
    10381 ggcggaaaaa ctggcgcgtg agctgctggc gaaagtcggt ctggcagaac gtgcacatca
    10441 ctacccttcc gaactttctg gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc
    10501 ggtgaagccg aaaatgatgc tgtttgatga accgacttcc gctcttgacc cggaactgcg
    10561 ccatgaagtg ctgaaggtta tgcaggatct ggctgaagaa gggatgacga tggtgatcgt
    10621 gacccacgaa atcggttttg ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg
    10681 ccggattgcg gaagatggca atccgcaggt gttgatcaag aacccgccga gccagcgctt
    10741 gcaggaattt ttgcagcacg tctcttaata agacacattg cctgatgcgc tacgcttatc
    10801 aggcctacag gatatctggc aacttattaa aattgcatga acttgtagga cggataaggc
    10861 gttcacgccg catccggcaa aaaagcccgc acgttgtcag caacctgctt aatatccctt
    10921 cctccctttc acccgaaagg gaggcacacc agattcctct catttaaaat cgcccctccc
    10981 ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc
    11041 ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat acccggcgtt
    11101 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
    11161 aaaaagcggc ctatggcgca ctggcggatg tgctggataa cgacacctcg cgtaaagagt
    11221 tgatcgacca gttgcgtact gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg
    11281 tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc
    11341 attatggtga agccctttcc gcccgcttcg ggcaacttta tcgcaatatc accggctccc
    11401 cgcataagcc atttaatcca caaaccttca gcaatgcgct gagccatttt tcaatgttag
    11461 cggtattagt gtttggtttt tactggctga ttcgcctgtg cgcactgccg ctatatcgca
    11521 aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag caactggttg cagcttccgg
    11581 cgatgattat tggggcgttt attatcgacc tgctgttact ggccctgaca ttatttgtcg
    11641 gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac catcgctttc caacaaagtt
    11701 tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc cgtactacgc ctgatttttt
    11761 gcccaaacgt ggcggagctg cgcccgttca cgattcagga cgagagcgcc cgttactggg
    11821 gtcgtcgcct gagctggtta agcagcctga ttggctatgg cctgattgtg gccgtgccga
    11881 ttatctctaa tcaggtgaat gtacagatag gtgcgctggc gaacgtcatc attatgctgt
    11941 gcatgaccgt ctgggcgttg tacctgatct tccgaaataa aaaagagatt acccagcata
    12001 tgctcaactt tgcggagcat tcgctggctt ttttcagcct gtttatccgc gcctttgcgc
    12061 tggtgtggca ctggctggca agcgcctatt ttatcatgct gtttttcttt tcgttgttcg
    12121 atccgggcaa cagtctgaaa tttatgatgg gtgcaacggt gcgcagcctg gcgattattg
    12181 gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct ggcgaaaacc atcaccctct
    12241 cgccacatac tcagcgtaac tatccggagc tacaaaaacg gctgaatggc tggctttcgg
    12301 cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc agtaatgttg ctgttgagcg
    12361 catggggatt gttcgatttc tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag
    12421 atatcctgat ccgcatcgca ctcattcttt tcttctcggc ggttggctgg acggtgctcg
    12481 ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg ccgcccgcta cccagcgccc
    12541 gtacgcgtac cctgctgacg ctgttccgta acgcgctggc ggtgattatc agtaccatca
    12601 ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg
    12661 gggcattagg tctggctatc tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg
    12721 gggtatttat tcagtttgaa aacggcatga acactggaga tttggtgact atcgggccgt
    12781 tgaccggcac agtggaacgg atgtcgattc gctccgtggg cgtgcgccag gataccgggg
    12841 cgtatcacat cattccgtgg tcttcgataa ccacctttgc taacttcgtc cgcggtattg
    12901 gttcggtagt ggcaaactac gatgttgatc gccatgaaga tgctgataaa gccaatcagg
    12961 cactgaaaga tgcggtaacg gaattgatgg aaaacgaaga aattcgcggg ctgattattg
    13021 gtgaaccgaa ctttgccggg attgtcggct taagcaatac cgcgtttaca ctgcgtgttt
    13081 cgttcaccac gctgccactc aaacagtgga cggtacgctt tgccctcgac agccaggtga
    13141 aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg
    13201 ctccgggcgc gaccccggct gaaccgctgc cgccggggga gccaacgctt taacgttggc
    13261 gattaacaaa acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata aagcgactct
    13321 gcttttgccc ctgggccatc tcttttttta ccaccttcac cgcgcccacg tcggtcagag
    13381 cacgatacaa cggcggtaag ttttcaccac gagataccag cgaggtaaac cacatcacct
    13441 gcttcgcgaa gcctttgctc tcttcaatca tctttttgat aaagttgact tcaccgcctt
    13501 cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg ttcagcccca
    13561 ggttacggcg tttacgctca ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg
    13621 ggttacacaa ggtcgcgtcg tattgctcgt ttttgtggat gatgccgtta aaaatcgccc
    13681 cactctcttt ttgccgacgc agacgaatgg cgcggttaag ccccggatta gcactgataa
    13741 tcgcctgcgc actgcttaac gcctggctgc tggtttcact accggtaaaa cgccagccat
    13801 attcatgtac gccaatcagc ggataaatac agttcgcacc aacgccgata tccagaatgc
    13861 tggcattcgc tggaattgtt ccgcttgctt cagccagtaa atcggcaagg tgatgaatat
    13921 aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta
    13981 cggcgtaaaa atgggccagc aacgccttat tgagcgcctt caccgccagc ggattggcaa
    14041 agtctacgct ttgctcccct gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga
    14101 cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg
    14161 gtttctgggc ggacatagca ttctccttta agcagcggcg taagataccc gcataacgcg
    14221 acagggtaaa tatctggctg cgtttatcat tccccaaaac cttaacatgg ctatggttta
    14281 tggcaattca tcataaaaat cagcattatg catgtcattg atttgtctta ttttgtaaaa
    14341 atacatcgtt caaaaaataa gcatttctct gtcaattttt tgcgtcattt gcatcgcgca
    14401 ggcgacctac acttatcagg cactacccgt tgtatttatg aggatgtcat tatgaaaaag
    14461 tgcctcacac tactgattgc caccgtcctg agcggaatct ctctcacggc ttatgccgcg
    14521 caaccgatga gtaacctgga cagcggtcag ctacggcccg ccggtaccgt ttcggcaact
    14581 ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg cgaacaaggc
    14641 gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttagg tactgcaacc
    14701 atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt
    14761 ttgtaatcaa aaaacatcaa atatgaacgc aatgtaaata aatgtatttc tttttcgcgc
    14821 aatgggtgat agaaaatcgc atcaagtgat aatgcttatc aaaattatta tcacttgcac
    14881 gaacactatc acgggattaa cagtggcatc gcatccgcag agatgctttc tcgtggcagt
    14941 gaaaatttca acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct
    15001 gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggtat tacgcctgtg
    15061 gcacaggcac tcgccgccga agggcaagct aacgcggatg acacgctggt tgtcgaagca
    15121 tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta
    15181 gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca
    15241 accaacctta ccgacgcgct caaaaacgtc cccggcgtg
//
LOCUS       DAJTMX010000098        14976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTMX010000098 DAJTMX010000000
VERSION     DAJTMX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.098"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO145_002970"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO145_002970"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5468019.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO145_002971"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO145_002971"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5468020.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="OO145_002972"
     CDS             complement(1939..2811)
                     /locus_tag="OO145_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5468021.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO145_002973"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO145_002973"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5468022.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="OO145_002974"
     CDS             complement(3503..4288)
                     /locus_tag="OO145_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5468023.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO145_002975"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO145_002975"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5468024.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="OO145_002976"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="OO145_002976"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5468025.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="OO145_002977"
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="OO145_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5468026.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="OO145_002978"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="OO145_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5468027.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="OO145_002979"
     CDS             8580..9482
                     /locus_tag="OO145_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5468028.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="OO145_002980"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="OO145_002980"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5468029.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="OO145_002981"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="OO145_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="OO145_002982"
     CDS             13180..14583
                     /locus_tag="OO145_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5468030.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..>14976
                     /gene="ompL"
                     /locus_tag="OO145_002983"
     CDS             14651..>14976
                     /gene="ompL"
                     /locus_tag="OO145_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5468031.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3674 a         3855 c         3735 g         3712 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 gatttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccaggttaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggcctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctgga
//
LOCUS       DAJTMX010000099        14425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTMX010000099 DAJTMX010000000
VERSION     DAJTMX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.099"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..132)
                     /locus_tag="OO145_002984"
     CDS             complement(<1..132)
                     /locus_tag="OO145_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5468032.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO145_002985"
     CDS             315..458
                     /locus_tag="OO145_002985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468033.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO145_002986"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO145_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5468034.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO145_002987"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO145_002987"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5468035.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVRKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO145_002988"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO145_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5468036.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO145_002989"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO145_002989"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5468037.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO145_002990"
     CDS             complement(7051..7659)
                     /locus_tag="OO145_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5468038.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO145_002991"
     CDS             complement(7730..8815)
                     /locus_tag="OO145_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5468039.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO145_002992"
     CDS             complement(8818..9180)
                     /locus_tag="OO145_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5468040.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO145_002993"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO145_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5468041.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO145_002994"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO145_002994"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5468042.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO145_002995"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO145_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5468043.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO145_002996"
     CDS             complement(13472..13681)
                     /locus_tag="OO145_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468044.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="OO145_002997"
     CDS             13965..14327
                     /locus_tag="OO145_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5468045.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3526 a         3844 c         3624 g         3431 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gcttttcgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac caaac
//
LOCUS       DAJTMX010000100        14316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTMX010000100 DAJTMX010000000
VERSION     DAJTMX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.100"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..3051
                     /locus_tag="OO145_002998"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OO145_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OO145_002999"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OO145_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OO145_003000"
     CDS             6768..7175
                     /locus_tag="OO145_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468046.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OO145_003001"
     CDS             7717..8805
                     /locus_tag="OO145_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5468047.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OO145_003002"
     CDS             8816..11095
                     /locus_tag="OO145_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468048.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="OO145_003003"
     CDS             11088..12224
                     /locus_tag="OO145_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5468049.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="OO145_003004"
     CDS             12221..14224
                     /locus_tag="OO145_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468050.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3754 c         3655 g         3361 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcac
//
LOCUS       DAJTMX010000101        14042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTMX010000101 DAJTMX010000000
VERSION     DAJTMX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.101"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..360
                     /locus_tag="OO145_003005"
     CDS             <1..360
                     /locus_tag="OO145_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HCR5468051.1"
                     /translation="QSAELRLHPQDLGEVQISLKVDDNQAQIQMISPHQHVRAALEAA
                     LPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVP
                     VSLQGRVTGNSGVDIFA"
     gene            465..929
                     /gene="fliL"
                     /locus_tag="OO145_003006"
     CDS             465..929
                     /gene="fliL"
                     /locus_tag="OO145_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5468052.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            934..1938
                     /gene="fliM"
                     /locus_tag="OO145_003007"
     CDS             934..1938
                     /gene="fliM"
                     /locus_tag="OO145_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5468053.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            1935..2348
                     /gene="fliN"
                     /locus_tag="OO145_003008"
     CDS             1935..2348
                     /gene="fliN"
                     /locus_tag="OO145_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5468054.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            2351..2716
                     /gene="fliO"
                     /locus_tag="OO145_003009"
     CDS             2351..2716
                     /gene="fliO"
                     /locus_tag="OO145_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5468055.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            2716..3453
                     /gene="fliP"
                     /locus_tag="OO145_003010"
     CDS             2716..3453
                     /gene="fliP"
                     /locus_tag="OO145_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5468056.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGCGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            3463..3732
                     /gene="fliQ"
                     /locus_tag="OO145_003011"
     CDS             3463..3732
                     /gene="fliQ"
                     /locus_tag="OO145_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5468057.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            3741..4526
                     /gene="fliR"
                     /locus_tag="OO145_003012"
     CDS             3741..4526
                     /gene="fliR"
                     /locus_tag="OO145_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5468058.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            4811..5434
                     /gene="rcsA"
                     /locus_tag="OO145_003013"
     CDS             4811..5434
                     /gene="rcsA"
                     /locus_tag="OO145_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5468059.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(5478..5666)
                     /gene="dsrB"
                     /locus_tag="OO145_003014"
     CDS             complement(5478..5666)
                     /gene="dsrB"
                     /locus_tag="OO145_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5468060.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            5829..6056
                     /gene="yodD"
                     /locus_tag="OO145_003015"
     CDS             5829..6056
                     /gene="yodD"
                     /locus_tag="OO145_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5468061.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            6354..7169
                     /locus_tag="OO145_003016"
                     /pseudo
     CDS             6354..7169
                     /locus_tag="OO145_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(7166..8860)
                     /gene="yedQ"
                     /locus_tag="OO145_003017"
                     /pseudo
     CDS             complement(7166..8860)
                     /gene="yedQ"
                     /locus_tag="OO145_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(9031..9213)
                     /locus_tag="OO145_003018"
     CDS             complement(9031..9213)
                     /locus_tag="OO145_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5468062.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(9292..10209)
                     /locus_tag="OO145_003019"
     CDS             complement(9292..10209)
                     /locus_tag="OO145_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5468063.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            10382..11302
                     /gene="yedA"
                     /locus_tag="OO145_003020"
     CDS             10382..11302
                     /gene="yedA"
                     /locus_tag="OO145_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5468064.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(11291..11761)
                     /gene="vsr"
                     /locus_tag="OO145_003021"
     CDS             complement(11291..11761)
                     /gene="vsr"
                     /locus_tag="OO145_003021"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5468065.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(11742..13160)
                     /gene="dcm"
                     /locus_tag="OO145_003022"
     CDS             complement(11742..13160)
                     /gene="dcm"
                     /locus_tag="OO145_003022"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5468066.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(13227..13767)
                     /locus_tag="OO145_003023"
                     /pseudo
     CDS             complement(13227..13767)
                     /locus_tag="OO145_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(13807..>14042)
                     /locus_tag="OO145_003024"
     CDS             complement(13807..>14042)
                     /locus_tag="OO145_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468067.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         3358 a         3706 c         3492 g         3486 t
ORIGIN      
        1 caaagtgcag agttgcgtct gcacccacag gatttaggtg aagtgcaaat ttccctcaaa
       61 gtggatgata accaggcgca gatccagatg atttcaccgc atcagcacgt acgcgccgcc
      121 ctggaagcag cgctgccggt actgcgtacg cagctggccg aaagtggcat tcagttaggg
      181 caaagcaaca ttagtggcga aagctttagt ggtcagcagc aggccgcttc ccagcaacag
      241 caaagccaac gcacagcaaa ccatgaacct ctggcggggg aagacgacga tacgcttccg
      301 gttcccgtct ctttacaagg gcgtgtaaca ggtaacagcg gcgttgatat tttcgcctaa
      361 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
      421 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
      481 taagcaagaa aagcaagcga tcgctttgga tcccgattct ggtattcatt accctcgcgg
      541 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gacgacaaag
      601 cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt acggtcaatt
      661 tgggcgatgc ggatcgcgtg ctttatatcg gcataaccct gcgactgaaa gatgaagcaa
      721 cccgctcgcg gctgagtgag tatttgccgg aagtccgtag tcgcttgctg ttactgtttt
      781 cacgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
      841 ttaaaaccac actttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
      901 tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
      961 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtatt
     1021 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
     1081 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
     1141 ttcaacctgc tgcgccgtag cccggacatt accgtcgggg ccatccgcat tcagccgtac
     1201 catgaatttg cccgcaacct gccggtgccg accaacctga acctaatcca cctgaaaccg
     1261 ctacgcggca cagggctggt agtgttctcg ccgagtctgg tgtttatcgc cgtggataac
     1321 ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt tacccatact
     1381 gaacagcgcg tcatcaaccg tatgttgaag ctggcgctgg aaggctatag cgacgcctgg
     1441 aaagcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt ggaatttacc
     1501 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
     1561 aacctgaccg gcgaatttaa tatctgcctg ccattcagca tgatcgagcc gctacgggaa
     1621 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
     1681 ctggtgcgcc aggtgcagca ttcacagctg gaactggtgg ccaactttgc cgatatctcg
     1741 ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc
     1801 gatcgcatca tcgcccatgt tgacggcgtc ccggttctga ccagtcagta cggtactctc
     1861 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
     1921 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
     1981 acgatctgtg ggctgaagcg ttgagcgagc aaaaatcaac cagcgaaaaa agcgctgccg
     2041 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
     2101 tgattatgga tattccggtc aagctgaccg tcgagctggg ccgtacgcgg atgaccatca
     2161 aagagctgtt gcgtctgacg caagggtcag tcgtggcgct ggacggtctg gcgggcgaac
     2221 cgctggatat tctgatcaac ggttatttaa tcgcccaggg cgaagtggtg gttgttgccg
     2281 ataaatatgg cgtgcggatc accgatatca ttaccccgtc cgagcggatg cgccgcctga
     2341 gccgttagtg atgaataacc atgctactgt gcaatcttcc gcgccggttt ctgctgagcc
     2401 actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
     2461 gctggtaaaa cggctgggat ttgcccctaa acgcactggc gttaacggtc tgaaaattag
     2521 cgccagtgct tcgctgggcg cgcgtgaacg ggtcgtggtg gtcgatgtgg aagatgcacg
     2581 gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc ccccttcagc
     2641 accaacggaa gagataccgc agaccgattt tcagtcggtc atgaaaaatt tgcttaagcg
     2701 tggcgggaga tcctgatgcg tcgtttgttt tctgtcgcac cggtccttct ctggctgatt
     2761 acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctacc tggctgtgga
     2821 caaagctggt cgctcccggt gcagacgctg gtgttcatca cctcgttgac gttcattcca
     2881 gcgattttac tgatgatgac cagtttcacc cgcatcatta ttgtttttgg tttattgcgt
     2941 aacgcgctgg gaacgccctc cgcgccacct aaccaggtat tgctggggct ggcactgttt
     3001 ttgacctttt ttattatgtc gccagtgatc gacaaaattt atgtcgatgc gtaccagcca
     3061 ttcagcgaag agaaaatctc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt
     3121 gagtttatgc tgcgtcagac ccgcgaggca gatttagggt tgtttgccag actggcgaat
     3181 accggcccgt tgcaggggcc tgaagctgtg ccgatgcgca ttttgctccc ggcctacgtg
     3241 accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcatccc ttttttgatt
     3301 atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt tccccccgcc
     3361 accattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg
     3421 gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc
     3481 atgatgatgg gaactgaagc gatgaaagtc gcgctggcac tggcagcccc gctgttgctg
     3541 gtagcgctgg tcacgggcct tatcatcagt attttgcagg cagccacgca gattaacgaa
     3601 atgacgctgt cgtttattcc gaaaatcatc gccgtattta tcgccatcat tattgccgga
     3661 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
     3721 atcatcgggt agcccgtaca atgatgcagg agacaagcga tcaatggctt tcctggttaa
     3781 gcctgtactt ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgcg ccaattctga
     3841 gcgaacgcag cgtgccgaaa cgggtaaaac tgggtctggc aatgatgatc acgttcgcca
     3901 ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct ctgtggctgg
     3961 ccgtacagca gatcctgatc ggcattgcgc ttggttttac catgcaattt gcctttgccg
     4021 ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg gctgtcattt gcgacatttg
     4081 tcgatcctgg cagccatctc aatatgcccg ttttagcgcg catcatggat atgctggcat
     4141 tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg gtcgatacct
     4201 ttcacaccct gccgattggt agcgaaccgt tgaacagcaa tgcgtttctg gcacccacca
     4261 aagcagggag tttgattttc cttaacgggc tgatgctggc gttaccgctc attactctgt
     4321 tgctgaccct gaatctggca ttaggtttac tgaatcgtat ggccccgcaa ttatccattt
     4381 ttgttattgg atttccatta actctgactg tcggcatctc tttaatggcg gcattaatgc
     4441 cgttaattgc gcctttttgc gaacatttat tcagtgaaat ttttaatttg ctggctgata
     4501 ttattagtga attaccatta atataattcc gtaacgttta tcatgctacc ctaaggatta
     4561 tccgaaaaat aatatctgcg aacatcttcc aggatactcc tgtagcgaaa tatttgtttt
     4621 aagctcactc acatatcgca acatttactt taagacaatt ccaggcaaat tatacaacac
     4681 tttacgggat agtaagtccg cctgaaaaat tgcgagagtg gcgcattagg tgacccatgt
     4741 tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac gcattgagtg
     4801 agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac taggtttaac
     4861 cgggtatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg aaaccgttga
     4921 tgaccttgcc atagcttgtg attcacagcg tccttcagtg gtgtttatta atgaggactg
     4981 tttcatccac gatgcttcta acagtcagcg catcaagcac atcattaatc aacatcccaa
     5041 tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat atctattggt
     5101 cagaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct
     5161 tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc
     5221 attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca
     5281 aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa
     5341 acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac tgacggataa
     5401 tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc
     5461 agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg atgccctgct
     5521 tcattaaaga accagatgcc gagcgggtag tcttccagcg caaccaggta cattgtgcct
     5581 tcactaaact cctcaactgc cagtaccact ccaggacgac gcggaccgcc atccgttttg
     5641 actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa
     5701 caatttcgtt tttctggcag cgccaagcgc gcgcgagtgc tgattttctc gacggtctat
     5761 acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa cggttgcgag
     5821 gaaagatgat gaaaaccgca aaagtgtaca gcgataccgc aaaacgtgag gtcagcgtcg
     5881 atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt catcgcagcc
     5941 agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg cgtgaattgg
     6001 cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat cgttgaatgc
     6061 acaataaaaa aatcccgacc ctgagggtgt cgggatgaaa cttgcttaag caagaagcac
     6121 ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatctg caattttttt
     6181 cgctgacaag aaaatattcg cgctatgaat gattaacgct acttatgaat agaaatgtga
     6241 cgcgcgtcac cccttggtgg ctctggtgtc aacgggatcg ctcccgaaaa aatcatcttt
     6301 gattatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgcg accatgtttt
     6361 caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg gacagtcata
     6421 gttatgactg gcaaccggca gccccctggc tcagccgttt acacgaagcc aatattcccg
     6481 tcattctctg tagcagtaaa actttagcgg aaatgctgta cttgcaaaaa atgttggggc
     6541 tacaaggctt accgctgatt gcagagaatg gcgcagtgat ccagcttgct gaacaatggc
     6601 aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa atctgccagg
     6661 ttttaaatac gctacgcgag aaagaacatt ttaaattcac gacttttgat gatgtcgacg
     6721 atgcaaccat tgccgaatgg acgggattaa gccgtagcta ggcggcgctg acgcagctgc
     6781 atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca caatttatcg
     6841 ctcgtctgaa cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg cacgtactgg
     6901 atgcctctgc cggaaaagat caggctgcca actggattat cgcgacctat caacaattgt
     6961 caggcagacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg cctttactgg
     7021 aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggta catctgcatg
     7081 atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc tggcgtgaag
     7141 ggctggacca ttttttctcc gcccgttaag cgttatcgct cgcgaatacc cgattgcgcc
     7201 cggcctgttt tgcgaggtaa agccgacggt cggccaatga ctggagttgt tcaaaatcat
     7261 aatcaccggt ttcctcgctg ctacttaccc ccagcgaggc actgatgcgt atcgttgtac
     7321 tcttggcgat cagcatctct ttttcattta acttcaggcg aatacgttct gcgacctccg
     7381 cagcctgcgt cagattcgcg cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc
     7441 cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga gaaagaacac
     7501 ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca agatcgacct
     7561 gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga cgggcttttt
     7621 cgaacagtgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc tgccactgca
     7681 acgagctttg cagaacatac atgttgctga ccatccggcg aatcacatac caggagagga
     7741 gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata ctgccgaaat
     7801 cgccgcgcac gccttcgctt agcgtatgga cgcgcgccag cacaccgtcg aaatggtcca
     7861 gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg tcatgttcca
     7921 ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt ggatggtcag
     7981 gattggaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca ccatcgaggt
     8041 ttttatcgat ggcgtttctt aaaaattgct gcatagtacg cacgggaata ctcatcccca
     8101 gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc tgcggttcag
     8161 tattgctggc gtgttccggt tgcgaagtga accagcgtac cgcgcggtga cgattttctc
     8221 gttgcgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc gttggtacat
     8281 tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc agcacgcgag acatacatcg
     8341 cctgttcaac catcgacgag gagttgtgcg ccagtcgcag caagtaacca acttccagtg
     8401 cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggaaattc ccctcgctga
     8461 ctaatgcatc cgatacgcca ttgactggca gggtgcgccg tcggttgagt tcgatttgcc
     8521 aggcgtgctc gtcgcgatgc tgttcgaact cggttacggc attacgcaga gaggtgaaat
     8581 ccagtggcgc cacgagagct tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt
     8641 tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta cgctagctgg
     8701 agatataggc atcttccagc accaccactt cacgccaggt gagcaaggtg gaaaaaagca
     8761 ataccacgat aaagcagaga ttaacgacat gaccgggccc caggcggcgt gcgagttttt
     8821 tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact cccaacctgc
     8881 ttttttatga ttctggcata acccggcaat agcgtaccgc aaaacagcga taattttata
     8941 atggtggcat gataagacgc gactatcatt cattgcaggc attgggacac caggccggat
     9001 gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac gactcccctc
     9061 ccccggaaca agatcggttt catgaaaagc ttcccgcttg accccgtaac cgtcatacca
     9121 acgacactca accataccgc tggagtatcc agtgacaatc atccgcgggc cgccctcttt
     9181 aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc gagtgaaact
     9241 tgttcacctt agttgaagat ggcgaatttt gcataatagc cagtgcgagt attaatgtgc
     9301 ctgaccgcgc attttcgcta ctgctttcac tcccagcacc acgatgccgc cgatgataaa
     9361 tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac tttgctgccc
     9421 ggcgatatgt tcaatagcgt gatgcagcgg cgcaatacca tgtaccacaa tcccgccgcc
     9481 gacgaggaac atcgccagcg tgccgacaat cgataatgct ttcatcagcc aaggcgcgat
     9541 aatcaataac cctttaccta atgcctgcat cagcgcgcta gatttttccg ccagccaata
     9601 ccctaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca cggtcactac
     9661 cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcctctg ccacaatccc
     9721 cagcgtgatg gcgacgattt ccgcagacaa aataaaatcg gtacgaatcg cccctttaat
     9781 tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac gctgctggct
     9841 ctgcgctgga tcttctttat gtttacgcgc ctccagcata tgcagcactt tctctacccc
     9901 ttcaaagcag agaaacgcgc caccaatcat caacagtggc gtaatcgccc acgggataaa
     9961 cgcactgatg atcagcgcca gcggcaccag aatcacttta ttgatcagcg atcctttcgc
    10021 cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt gttgcgcatt
    10081 gagcgataaa tcatccccta atacaccggc ggttttcttc gccgccagtt tgcccatcac
    10141 agagatatcg tccagcagtg tggcgatatc atcgagcaac gtcagtaaac tactcccggc
    10201 caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca aaaacggcag
    10261 ctctgatact tgcttcacag gtcaacattt atttaacaat caagtgcaat tatatctctc
    10321 gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccgc cgtgagggat
    10381 tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt atatcatttg
    10441 gggctcaacc tattttgtca ttcggattgg cgtgggaagc tggcctccgt taatgatggc
    10501 gggcgttcga ttccaggcag ccggtatttt attgctggca tttttgctac tgcgcggaca
    10561 caaacttccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt tattgctggc
    10621 tgtcggtaat ggcatggtga cggttgccga acatcaaaat gttccttccg gcatcgccgc
    10681 cgtagtgatt gcaaccgtgc ccctctttac cctgtgcttc agtcgcctgt ttggcattaa
    10741 aacgcgcaaa ctggaatggg tgggtattgc cattgggctt gccggaatca tcatgctcaa
    10801 tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa tcggctcgat
    10861 tagctgggcg tttggctcag tttatggctc gcgcattacc ttacctgtag ggatgatggc
    10921 gggtgcgatt gagatgctgg cggcaggcgt ggtgttaatg atcgcatcga tgattgcggg
    10981 tgaaaaactg acggcgctcc cttccctgtc aggcttcctt gcggtcggct atctggcgct
    11041 gtttggttcg attatcgcca ttaacgctta tatgtattta atccgtaatg tcagtccggc
    11101 tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg gtacgggact
    11161 gggtggcgaa acgctgtcga agattgaatg gctggcgctc ggcgtaattg ttttcgcggt
    11221 ggtactggtc acgttgggaa aatatctctt cccggccaaa cccgttgtcg cgccagttat
    11281 tcaggatgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg ctggcacctt
    11341 cgccgcagat ccactcttcc agacgctcgg taagcgccgc atccgtcagc ttctcgcgcc
    11401 tacgtaacgc acactcccag acaatcagta cgcgccagcc gagctcctgc aagcgactga
    11461 tatcgcggcg atcgcgttca acatttttac ctatcttctc cagccagaac tcagttcgag
    11521 tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg tgggtaaata
    11581 tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg gcgtcctgaa
    11641 cgcgaaacgc aaggccctgc ccggttaaca aactggcgag gcgcttctca atcgccgtat
    11701 cacgcgtggc aatcgcgcgc atatttttgc tgcgggtggc cttatcgtga acgtcgacca
    11761 tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc aagcagtttt
    11821 gccaccgcgg caaagaccgg cacgaccacc gagttgccga actggcgata ggcctgagtg
    11881 tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat taagcgcgcg
    11941 cattcacgag gcgttaaccg acgtggacga tgttgctgat tcagcggatc gtcaaagtct
    12001 ttctcacccg tggccatatc ccaaccgcga tcgattaaaa tttccgcgcc atctttgtaa
    12061 taacgagcag acagcgtacg ggtgacgctt tgcggattgt tcggataaac cattccataa
    12121 ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta cttccacagc
    12181 accggcgtca ggatatattt cgcctcaacc atcgggtcca gcagctgcgc cagcgtcact
    12241 cgctgtgcag ggaaacattc gctgatatca cgcagagtaa aatcggcttt cagattcaga
    12301 tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggcaggaa atgtttacca
    12361 tcgatgattt tcggatcgtc cggcccgtta tcttctgcat cggcaacgtc atagcccagt
    12421 tcgtccagcg tctgcatgat gatgcggaac gtttttccct tatcgtgact cttcaggttt
    12481 ttgacgtttt cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg caccacatca
    12541 aagaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag cgagttcttt
    12601 ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa aacatcgtgt
    12661 tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc ttcttgatga
    12721 ctgagggtga tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc gcaataatgg
    12781 ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac gcactgtcca
    12841 ccaatcgatt caaaaccacg acggatgccg ccaattccgg caaatagatc gataaagcga
    12901 aacgcataat gcggatgatg ttccggtggt ttgggtaaca acgtttgcag atgggcaaac
    12961 tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt taaaattgcc
    13021 gcgctccagt gattctcacc cacaccatta agctgcgcca ccagcatttt aacatcataa
    13081 atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta tgaatcggtt
    13141 actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac aggttaacat
    13201 aattactgcc gtttagggcc acgcgctcag ccagcgggtg aaaacgcatc tatcaccttg
    13261 tgatcgatac cttcattctc ccccgccagt tcggcaccga gcttcgccat caaacagcgc
    13321 caagctcaat gcccctgaaa cggcaaatac gcgcgccagg ccaatcgccc ccagggcttc
    13381 cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga tttgcgcgct
    13441 gaaactgtga gcggcaatgg catgacaaac ggcctcaatt ttctccgccg gaaattgcac
    13501 gaactcttca cggagcaggc gacgcgtctc ttctgccgcc aggattgaag aacgctgccg
    13561 ttgcggatga tttttcgcca ggctcacgat atcgtgaaaa taacaggcgg ttaaaatcac
    13621 cagcatatcg acatcgtcat ctgccgccag tttctgtgcc gtagcccaga cacggcgaaa
    13681 atgacacagg tcatgcgccg cgtcctgatg ttgatgatgg tttttcaacc agttctcgaa
    13741 ctatgcctgc cagtgttgta actccatacg cgctccgttg ccaaagagtc tcaaccttag
    13801 cagtttttat tcatagcgtc tgcaacgtgc gggacgaggt cgggtgtacc atgcaatgcc
    13861 gcctaacaag gcaccaacca gggccataaa cagaaatccg accaatgctc ctaaccactt
    13921 ccccgcggta gatcctaaat cactttcaaa tcccgacacg ggggcatcgg ggatccggct
    13981 catcacccag atgtatcgcg ccattgccca gacgaaatag ccacaccatg tatagaaagc
    14041 cc
//
LOCUS       DAJTMX010000102        13964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTMX010000102 DAJTMX010000000
VERSION     DAJTMX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.102"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO145_003025"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO145_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5468068.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO145_003026"
     CDS             2219..2767
                     /locus_tag="OO145_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5468069.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO145_003027"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO145_003027"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5468070.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO145_003028"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO145_003028"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5468071.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO145_003029"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO145_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5468072.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO145_003030"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO145_003030"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5468073.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAMTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO145_003031"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO145_003031"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5468074.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO145_003032"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO145_003032"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5468075.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO145_003033"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO145_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5468076.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO145_003034"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO145_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5468077.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3562 c         3526 g         3325 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcat tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTMX010000103        13250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTMX010000103 DAJTMX010000000
VERSION     DAJTMX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.103"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OO145_003035"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OO145_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5468078.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO145_003036"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO145_003036"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5468079.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OO145_003037"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OO145_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO145_003038"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO145_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5468080.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO145_003039"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO145_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5468081.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OO145_003040"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OO145_003040"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5468082.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OO145_003041"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OO145_003041"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5468083.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OO145_003042"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OO145_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OO145_003043"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OO145_003043"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5468084.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="OO145_003044"
     CDS             9657..10103
                     /locus_tag="OO145_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5468085.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="OO145_003045"
     CDS             10114..11565
                     /locus_tag="OO145_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5468086.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="OO145_003046"
     CDS             11555..12625
                     /locus_tag="OO145_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5468087.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13250
                     /locus_tag="OO145_003047"
     CDS             12625..>13250
                     /locus_tag="OO145_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5468088.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3159 a         3459 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggacca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaata
//
LOCUS       DAJTMX010000104        13142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTMX010000104 DAJTMX010000000
VERSION     DAJTMX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.104"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(87..794)
                     /locus_tag="OO145_003048"
     CDS             complement(87..794)
                     /locus_tag="OO145_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5468089.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1192..3327
                     /gene="speC"
                     /locus_tag="OO145_003049"
     CDS             1192..3327
                     /gene="speC"
                     /locus_tag="OO145_003049"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5468090.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="OO145_003050"
     CDS             complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="OO145_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5468091.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="OO145_003051"
     CDS             complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="OO145_003051"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5468092.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /locus_tag="OO145_003052"
     CDS             complement(5978..6253)
                     /locus_tag="OO145_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5468093.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="OO145_003053"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="OO145_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5468094.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="OO145_003054"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="OO145_003054"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5468095.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /locus_tag="OO145_003055"
     CDS             8213..8539
                     /locus_tag="OO145_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5468096.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            8723..9442
                     /locus_tag="OO145_003056"
     CDS             8723..9442
                     /locus_tag="OO145_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5468097.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="OO145_003057"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="OO145_003057"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5468098.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11743
                     /locus_tag="OO145_003058"
     CDS             10781..11743
                     /locus_tag="OO145_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5468099.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(11844..>13142)
                     /locus_tag="OO145_003059"
     CDS             complement(11844..>13142)
                     /locus_tag="OO145_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5468100.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3451 a         3175 c         3403 g         3113 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtga tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgctggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggcgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcgcatt tcacaaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga cgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaaga ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttagc tgtattgttt gattttctta ttatgaactt
      841 tttgccttgt tgatggcttg tctggtcgca aagtcctggc ttgcacgcct tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccatctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgaactggta tcccgacttt cttctcatcg ccgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 cagtggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttctttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cggggacgac gccggtctat ctggaagctt cacgtaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagccga cctgccgcgc ccgtttcgcc tgacgattat tcagctggga
     2041 acctatgacg gcactgtcta taacgcccgt caggtgatcg ataccattgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttacgaac agtttatctc gatgatggcg
     2161 gatagctcgc cgctgctgtt agaacttaac gaaaacgatc cggggatttt tgtgacccag
     2221 ttggtgcaca aacagcaggc tggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataacgc ctttatgctc
     2341 catgcttcta ccagcccttt ctatccgctg tttgctgcac tggatgttaa cgccaaaatt
     2401 catgaagggg agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtaagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatca gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ccgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaaccgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtggcgat gctggcgcaa tttgaacagc atattgagga tgactcgtcg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 ctgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcgctgg aagaaggcgt gaatttgttg
     3241 ccgggatttt cgccggagtt gcaaggtgtc tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cgattgtacg gttatgtgtt gaagtaagaa taaaaaaacg ggtcaccttc tggcgacccg
     3361 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
     3421 atatttgaac atcgccatga acgcgaaggc cagaaccacg gaataaccag cgaaaatcaa
     3481 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
     3541 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
     3601 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
     3661 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
     3721 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
     3781 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
     3841 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacgatga agctgctggc
     3901 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
     3961 cagttccgcg cccagcagca ttgagaagat gaagaagatt gccatacgct tgtttttaaa
     4021 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
     4081 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc
     4141 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
     4201 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
     4261 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
     4321 aaacgagtta atcaatatca caaggaacat cgcttccggc gtcgtgacct gtgccgccat
     4381 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
     4441 tttgtcggcc acaatcccca gcagcgcagg cataaatacc gcggcgatac ccagtgagct
     4501 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag
     4561 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
     4621 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
     4681 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
     4741 gcaaataatt gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt
     4801 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
     4861 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
     4921 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
     4981 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
     5041 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
     5101 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
     5161 tttcccctgc gagcggaaca catctttccc ggcagtattt tgtaccacct gcattaatcc
     5221 cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcagtct gcataattgc
     5281 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
     5341 tttgtgcgca cgtttatcaa ggtggttcgg caccatgtta atggtgacgc tgtagatgat
     5401 acgtagcccg ttgctgcggc ttttcagacg gtttttcagc agataatccg cgaagttgct
     5461 tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
     5521 aggttctttc gaaattgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
     5581 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
     5641 ggcgatggtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
     5701 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaagcccca
     5761 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
     5821 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
     5881 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
     5941 ttatgcaagc tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca
     6061 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaactgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tggaaatggc tgaaggtatg gcgaaacgcg gtcagttgcg
     6481 tcaggttatc ggcagcaatc tgccgttgtg ccagccaatg ccgcaaactt tcttcgtcgg
     6541 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aacaggagaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgaccagc tattgttggt ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggcttcg aacgcgtgca aatcatcgcg cccaaatcca
     6781 tcatcgcctg attaaaccgt tccacgccga ccgcgggcgt cacctgctcg ctcaaactcc
     6841 atagtttatt ctcgacctct tttttcccag gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgcgcctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
     7021 ggaatttacc accgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgtcgggaag tgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gacttcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
     7621 cggtgatggg cgttgagttc agtgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc cgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgttcaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaatcgttga
     8341 tgactttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagtgcaa actggatgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
     8581 gctacgctta tcaggcctac agagatgcac gatcgagtag gcgggataag gcgtttacgc
     8641 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcat atgctggcct
     8701 gatttgttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tacgtagcac gctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgag aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgacag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag cttaaagagc agatgaatcg
     9121 cattatcgaa acgcgcagcg atggcctgac gttccactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaactag tgaatcaggc aatgggcgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
     9301 aagcttgggc ggtctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccatg caaagctaag ggataatgcg tagcgtccac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacgccga ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaactgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca
    10141 ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgattac caacgtaccc
    10261 cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga
    10321 aagtcggcat cgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg
    10441 tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa
    10621 ccaaagatcc gcagcagatc cagcagattt tcaatcagta ctaatcacct cgtcccggca
    10681 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    10741 ataatcaccg gaataaatta ttccgcgcga gggttttcgg gtgaaaaagc aatggattgt
    10801 tggcacggcg ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    10921 catcagctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    11041 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    11281 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    11401 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    11641 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    11701 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    11761 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    11821 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    11881 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    11941 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    12001 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    12061 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    12121 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    12181 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    12241 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    12301 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    12361 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    12421 gtaaaaatgc cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag
    12481 gcatccagaa acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca
    12541 cggcgtttag caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc
    12601 aaatatcccg ccataaacag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca
    12661 attaacacgt ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg
    12721 gtagaaaatg ccggatcata accttctttg gtctgaatgg gattcagtgt gccaccaacg
    12781 gcagcagctg ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg
    12841 ttcacatgac caagcgaacc aggaacgcgg ccaaccatta cctgggcgag gttaatcaaa
    12901 cgtatcgcaa taccgccgct gttcatcagt gttccggcaa agatgaaaaa ggggattgcc
    12961 aacagggcaa agttatccag tccattagcc agtcgttgcg aaacagtgat tgccgcgata
    13021 tcccagggaa acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg
    13081 ggcacaccaa taaagaccag cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta
    13141 tc
//
LOCUS       DAJTMX010000105        13098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTMX010000105 DAJTMX010000000
VERSION     DAJTMX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.105"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..890)
                     /locus_tag="OO145_003060"
     CDS             complement(<1..890)
                     /locus_tag="OO145_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5468101.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO145_003061"
     CDS             1131..1931
                     /locus_tag="OO145_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5468102.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO145_003062"
     CDS             2009..2779
                     /locus_tag="OO145_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5468103.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO145_003063"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO145_003063"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5468104.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO145_003064"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO145_003064"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5468105.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OO145_003065"
     CDS             5034..5756
                     /locus_tag="OO145_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5468106.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO145_003066"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO145_003066"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5468107.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO145_003067"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO145_003067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5468108.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OO145_003068"
     tRNA            7149..7225
                     /locus_tag="OO145_003068"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OO145_003069"
     CDS             complement(7356..8402)
                     /locus_tag="OO145_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5468109.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO145_003070"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO145_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO145_003071"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO145_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5468110.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO145_003072"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO145_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5468111.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OO145_003073"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OO145_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO145_003074"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO145_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAJTMX010000106        12908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTMX010000106 DAJTMX010000000
VERSION     DAJTMX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.106"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO145_003075"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO145_003075"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5468112.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO145_003076"
     CDS             complement(1806..3224)
                     /locus_tag="OO145_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5468113.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO145_003077"
     CDS             complement(3378..3722)
                     /locus_tag="OO145_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5468114.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO145_003078"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO145_003078"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5468115.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO145_003079"
     CDS             complement(4712..5647)
                     /locus_tag="OO145_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5468116.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO145_003080"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO145_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5468117.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO145_003081"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO145_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5468118.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO145_003082"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO145_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5468119.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSSNIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO145_003083"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO145_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5468120.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO145_003084"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO145_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5468121.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO145_003085"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO145_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5468122.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO145_003086"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO145_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5468123.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO145_003087"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO145_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5468124.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO145_003088"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO145_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5468125.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3402 a         3298 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggaactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAJTMX010000107        12769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTMX010000107 DAJTMX010000000
VERSION     DAJTMX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.107"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(280..999)
                     /locus_tag="OO145_003089"
                     /pseudo
     CDS             complement(280..999)
                     /locus_tag="OO145_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1015..1347)
                     /gene="cusF"
                     /locus_tag="OO145_003090"
     CDS             complement(1015..1347)
                     /gene="cusF"
                     /locus_tag="OO145_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5468126.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1371..2753)
                     /gene="cusC"
                     /locus_tag="OO145_003091"
     CDS             complement(1371..2753)
                     /gene="cusC"
                     /locus_tag="OO145_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5468127.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2910..3593
                     /gene="cusR"
                     /locus_tag="OO145_003092"
     CDS             2910..3593
                     /gene="cusR"
                     /locus_tag="OO145_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5468128.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3583..5025
                     /gene="cusS"
                     /locus_tag="OO145_003093"
     CDS             3583..5025
                     /gene="cusS"
                     /locus_tag="OO145_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5468129.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5175..7412
                     /gene="nrfB"
                     /locus_tag="OO145_003094"
     CDS             5175..7412
                     /gene="nrfB"
                     /locus_tag="OO145_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5468130.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7399..10367
                     /gene="nfrA"
                     /locus_tag="OO145_003095"
                     /pseudo
     CDS             7399..10367
                     /gene="nfrA"
                     /locus_tag="OO145_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10368..11258
                     /locus_tag="OO145_003096"
     CDS             10368..11258
                     /locus_tag="OO145_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5468131.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11441..12202
                     /gene="envY"
                     /locus_tag="OO145_003097"
     CDS             11441..12202
                     /gene="envY"
                     /locus_tag="OO145_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5468132.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12248..12324)
                     /locus_tag="OO145_003098"
     tRNA            complement(12248..12324)
                     /locus_tag="OO145_003098"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12288..12290),aa:Arg,seq:tct)
     gene            12567..>12769
                     /locus_tag="OO145_003099"
     CDS             12567..>12769
                     /locus_tag="OO145_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5468133.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3125 a         3313 c         3238 g         3093 t
ORIGIN      
        1 gcatcagcca attcttgtgg gccagaatat ggtttaccat aaatagtacg cagttttgtg
       61 tcgcttatag ccataaataa tatttggtac gctcagaaat aatggtttgg tacacatctg
      121 gtacacaata tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac
      181 atactcaggt gaaaaaaagg ttgtttttcg agttataaca gacagttaat taactacagt
      241 ggacaatgct agacaatgac agacacagat aagcaaccta ccttcctctt tcgcgaccac
      301 gttatctttg gcgatattca ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat
      361 gggcgctttg agcgtaaagc gagtctggat tttttgcgtg gcgatcaggt ggcgaatatc
      421 cgcctccggc attcccgcca gtcgcagccg ctcaagaatg ccttcagtct gggtcgccgt
      481 accgccggtt tcgcgcaaca gtaaatactc actctgcgct tccacccagt cgggaatggt
      541 caggtcgaga agcggtgtgc ccttttgtac tttatcgccc acggtaagcg gatacacctt
      601 gtcgataaac ccggcagcgc gagcctgcac aatggcatac tgatactcgt tgtaactgac
      661 attcgtcggg aaactctggg caaaagtcag cggcccgcgc gtgacggtag ccgttttcac
      721 ccccagattc tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac tctcttcatc
      781 ggcatatttc ggcaccagat ccatatccat aaacggcgat ttacctggtt tatcgaaccg
      841 cgtattggga tacatcgggt cgtaccagaa taagatttta cgttctgcgg tcggcatttt
      901 ttctgcgtgc ggttccgcct ggtcaaacca ggtaaaacct gccgctgaaa taataccgcc
      961 cgcgatcatg ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg
     1021 gctgacttta atatcctgta ataaagaaag gttgccctgc tggacaaaat taaacgccac
     1081 tttgtcgccg gttttaattt cactcatttt cgtctgcggg gtgatggtaa agcgcatggt
     1141 catctccggc cagttgacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc
     1201 cagatcaaca ccttttacca caccggtggc actaatgacc tgtggttgtg cttcgctcat
     1261 ggtttcatga tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactgaa
     1321 cattgcgact tgcagtgctt ttttcattta ttctctcctg gagttaaaaa ttattgctgc
     1381 caaccgcccc ctaaggcggt atacaaataa atttcgttaa cctgacgggc ataattcagg
     1441 tcaagtaaag tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa
     1501 cttaccgcgc cgtgctgata taatgcccgc gcccgttgca gggtaatttg cagcgacgcc
     1561 agataacgct gctgggcgct gatttgatcg tccaggcttt gacgtaatgc cagcgcatct
     1621 gccacctctt taaaggcgtt ctggattttc tgttcataat tcaccaccga ctgctgctgg
     1681 cgaatttcgg cgatatccag attggcctgg ttgcgtccgg cattaaaaat ggggatctca
     1741 attttaggaa taaaattcca catcccgctg ctagcgttaa ataatgacga cagatcgctg
     1801 ctggcggttg atattccgct ggtcaggctg atagacggga aaaatgccgc acgcgcagca
     1861 ccaatattag cattagccgc cattaacgcg tgttcagctt ccataatatc cgggcgctgc
     1921 aataagattt gcgacgacaa gcccgccggt aatttaacgc tttgcaggct gccgctgttt
     1981 actgtctgcg cttgcggtag cttgccgtag cttcccaata acagttgcaa tgcattattc
     2041 gcctgcgcca gttccccctg acgtttagcg atgtcactgc gggtactttc tatcaccccg
     2101 cgagcctgtt ccagcgccag aacattgctg ctaccggtca acagttgttt ttcgacaaac
     2161 gcatatgact gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc
     2221 agctgctgat tgaaatagct ttgcgcgaca ttagaaacca gcaaaatatg caccgcgcgc
     2281 tgggcttcct cagtggctaa ataattttgg cgctcggctt cgctcatgtt ctttaagcga
     2341 ccgaaaaaat cgagatcaaa gctggcgtta aggccagtcg agaactcccg cgtcgtggct
     2401 gaatcgcctt taagattgcc gctccagctg ccgctgccct cgccattgag ctgtgggtag
     2461 cggtcggcat cggtcagacg atattgcgcc cgtgcctcct gcactttcag cgtcgccatg
     2521 cgcaaatccc ggttattcac cagcgcctcg ctaatcagcg tcttcacctg attatcaaca
     2581 aaaaaggtgc gccagcccac gttctggtag ttatccgctg cgttaaccag gccgttctgg
     2641 ctgagtgaga actgctgcgg tacgggcatt accggacgct gataatccgg tgccagtgaa
     2701 caaccagtta gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca
     2761 taatttctgg tgattttatg tcgccaactt tactcgccag gctctgattt tccggtgaca
     2821 ggaaaatgac aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta
     2881 gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga
     2941 aaaccggaga atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg
     3001 acaacgggct gaatggctac catctggcga tgaccggtga ttatgacctg ataatcctcg
     3061 atattatgct gccagacgtg aacggctggg atatcgtgcg catgttgcgc tccgccaata
     3121 aagggatgcc gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc
     3181 tggagttggg ggcagatgac tacctggtga aaccgttcgc ttttgctgaa ctgctggcgc
     3241 gggtgcgcac tctcctgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg
     3301 ccgatctgat ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactc
     3361 tgaccagtaa agaatttact ctgctggagt tttttctccg ccatcagggc gaagtgctgc
     3421 cccgctcgct tatcgcctcg caggtatggg acatgaattt cgacagcgac actaacgcca
     3481 ttgatgtggc ggtgaagctg ctacgcggca aaatcgacaa cgactttgag ccgaagctga
     3541 ttcaaaccgt gcgcggcgtg ggttacatgc ttgaggtgcc ggatggtcag taagccattt
     3601 cagcgcccgt tttcgctggc aacccgcctg acctttttta tcagccaggc caccatcgcg
     3661 gcgtttttcg cctttgcatg gatcataatc cactcagtaa aagtgcattt tgccgagcag
     3721 gacattaatg atttaaaaga gattagcgcc acccttgaac gggtactaaa tcaccctgac
     3781 gaaacgcaag cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaacgtg
     3841 ttgatttccc tggcagatag ccacggcaaa acggtgtatc actcccccgg tgcgcctgat
     3901 atccgcgagt ttgcacgtga tgccataccc gataaagacg cgcggggtgg cgaggtgtat
     3961 ctcctttccg gcccgacgat tatgatgcca ggccacggtc acgggcatat ggaacacagc
     4021 aactggcgga tgattaactt gccggttggc ccgttggtgg acggcaaacc gatttatacg
     4081 ctctacatcg cgctgtcgat cgattttcat cttcattaca taaatgattt gatgaataaa
     4141 cttattatga ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct gttggcggta
     4201 cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa
     4261 gatctcgacg ttcgccttga cccgcagacc gtgccgattg agctggaaca gctggtactg
     4321 tcgttcaacc atatgatcga gcgtattgag gatgtcttta ctcgccagtc caatttctca
     4381 gcggatatcg cccacgaaat tcgcacacca attactaatc tcattacgca aacggaaatc
     4441 gccctcagcc agtcgcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa
     4501 gagctgacgc gaatggcgaa aatggtcagc gatatgctgt ttctcgctca ggccgataac
     4561 aaccagctaa tccccgaaaa gaaaatgctc aacctggcgg atgaagtcgg caaagtgttc
     4621 gattttttcg aggcgctagc ggaagatcgc ggcgtggagt tgcggtttgt tggcgacaaa
     4681 cgtcaggttg cgggcgatcc gttgatgctg cgtcgggcgc taagcaacct gctttctaat
     4741 gcccttcgtt atacgccaac cagagagaca attgtagtac gctgtcagac ggtcgatcac
     4801 caggtgcaag tttccgtcga aaaccccggt acgcccattg cgcccgagca cttaccgcga
     4861 ttgtttgacc gtttctatcg cgttgacccc tcccgccagc gaaaaggtga aggtagcggt
     4921 attgggctgg cgatagtgaa atcgattgtt gtcgcgcata aaggcacggt tgcggtaacg
     4981 tcagatgcgc gggggacaag gtttgttatc acattacccg cttaattatt cagcaaacgg
     5041 caacttttat aaccagttta aaaataacgt gccgcaatag tcatacatat taatggtaaa
     5101 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
     5161 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
     5221 aagtaatcgc gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta
     5281 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
     5341 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
     5401 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
     5461 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat actcaacgtg
     5521 atgttgacga agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg
     5581 gtcccaccag caaagccgac tgcctgaaca acgtgctgga cgccatcacc cagtttgaac
     5641 gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac
     5701 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccag
     5761 tgtatccgtt tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt
     5821 cagaactgca tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg
     5881 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc actgttagct gacggtaacg
     5941 gtattgcttt cgacgtgcag agtcttactg aagattacga cattggcttc cgcctgaaag
     6001 aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg
     6061 agcagcgtaa atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt
     6121 tccccgatac cttttcgact gcggttcgac aaaaatctcg ctggatcatc ggcattgttt
     6181 tccagggctt taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc
     6241 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc
     6301 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
     6361 tttttagcgg cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta
     6421 accgcatcgt gcagcgagtg attttcgtca ctggctacta cggcctcacg cagggcctgc
     6481 tttccgtcct gcgtcttttc tggggcaacc tgattaactt tatggctaac tggcgcgtgc
     6541 tgaaacaggt tcttcagcac ggcgatccgc gtcgcgtcgc gtgggataaa acaacgcatg
     6601 acttccccag cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg
     6661 aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc
     6721 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg
     6781 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct
     6841 cgctgattgc cgaaatgcca gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc
     6901 tggaaaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
     6961 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
     7021 tcacagggtt acgccactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
     7081 acaatgcggt tcagcatcag tggctcacgg aacagcagac cggtgaaatc tggcggcaat
     7141 atgtgccgca tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt
     7201 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
     7261 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
     7321 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg
     7381 cgcaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
     7441 ttactgctga cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
     7501 gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
     7561 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
     7621 caacaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgtcatttt
     7681 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat
     7741 gcccgacttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgactgtt
     7801 gaagaactgc ttgcccagca aaaagcgggc gacgctgcgc cgaccctgcg ttgtcgcagt
     7861 gaagtcgggc agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac
     7921 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg
     7981 gcaatctacc tgaaacaatg atcccaggca gatacgctat acaatgaagc acgccagcag
     8041 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg
     8101 gacgatcgga tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt
     8161 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
     8221 gaaaataagc cgctgtttac cacgggcgca caagagaaaa gttggctcta tctgttatct
     8281 aaatacagcg ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac
     8341 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca
     8401 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc
     8461 agcgtggcga cccgtaacaa ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa
     8521 gaaccgacaa atcttccccg cctggatcaa ctgacctggc aactgatgca gaacgagcag
     8581 tcacgcgaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc
     8641 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctagcaacg
     8701 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa
     8761 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
     8821 tcgccttcct acgatgctgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
     8881 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgag cgcctggcaa
     8941 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
     9001 tggcagaaaa tcagtcttca cgacatgagc aatgatgatc tgctcgccgc cgccaacacc
     9061 gctcaggcag cgggaaatgg cgcagctcgc gatcgctggc ttcaacaggc agaacaacgt
     9121 gagctgggaa acaatgctca atactggtgg ctgcatgcgc gacgttatat cccagctcag
     9181 ccagaactcg cactgaacga tctcacgcgc tcaatcaata ttgccccttc tgccaactct
     9241 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg ttccggccgc ggtgagtgat
     9301 ttgcgcgccg cgctggaact ggaaccaaat aatagcaaca cccaggcagc gcttggttac
     9361 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
     9421 gggcttccgg acgatccggc actgatccga caactggcct acgtgaacca gcgtctggat
     9481 gacatgcctg cgacgcagca ctacgcccgg ctcgtgattg atgacattga taatcaggca
     9541 taaccccact gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg
     9601 aggtcggtcg ccgctggacg ttcagtttcg attcttccat cggcttgcgt tccggcgcaa
     9661 tgagtaccgc taacaataat gtcggcggtg cagcgcctgg gaaaagctat cgtagctacg
     9721 gacaactgga agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct
     9781 cagtttatag ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa
     9841 atccgatgtc cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg
     9901 ccgtcgaaca gcagttgccg ctgaacgggc aaaatggcgc atccgatacc atgctgcgcg
     9961 ccagcgcctc attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag
    10021 gctggtttgc ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg
    10081 cgtggacggc agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg
    10141 agccttacgc tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc
    10201 ttggcggtgt cggggtccgc tggaatattt ggaccggcga gacgcactac gacgcctggc
    10261 cgcacaaagt cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta
    10321 acggagagcg caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca
    10381 ttttcgtatt gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta
    10441 tctggcaacc acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga
    10501 gtcagttacg ttttcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg
    10561 catttaccca gccagaacag cgcacgttat tgtttaagcg tgccgcagct gcgcaacaga
    10621 ctggcctgaa gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac
    10681 agtcgtctgc agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag
    10741 ccagattatg gagcgccgcg cctggcataa cgccggatgg ctggtacatc agcgcggaaa
    10801 ttgacgacct gaactggcgc agcgaagccg cccgtcaacc tttgctaaca tggttaaaca
    10861 acgcgcagcg gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg
    10921 ccggaaacat gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggag
    10981 ttaatgtctg ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac
    11041 gttatctaca ggccagcgcc gattgccaaa gctccgcccc agcaagcggc attgtttatg
    11101 aactgtttgt cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gacgcagaaa
    11161 ttgcctcgct gttagcgaaa cgttcctctt gcggtaaaga catgctctat ttctctctgc
    11221 gctatttgcc cgtcgcgcac ggcattctcg agtattaaat cccctccagg taagtcgggt
    11281 acgacctggc ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag
    11341 catgctaaag aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata
    11401 gatcctttga gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga
    11461 accttgcgtg gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac
    11521 gtttaccctt ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact
    11581 ctcaacgtta aatcacgtat taatcaccca catcaaccgt aacatcatca acgattatct
    11641 gttgttttta aataagaaat taacctgtgt aaagccctgg tcgcggctag caaccccggt
    11701 tatcgcttgt catagccgta caccggaagt gttccggcta gccaccaacc acagcaagca
    11761 gcaatccagc aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc
    11821 taactttctt gagcaatcgc ggtttattgc cctactgatg tatatcttac gcagcagcgt
    11881 ccgcgacagc gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat
    11941 tgtcgccagt tcgctatgtt taagccccag cctgctcaaa aagaaattaa aaaacgaaaa
    12001 taccagctat agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt
    12061 gatggataac aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta
    12121 ctttatctct gtttttaagg cgttttacag cctgacaccg ttgaattatc tcgccaaaca
    12181 gcgacaaaaa gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa
    12241 gaagaaatgg cgcgccctgc aggattcgaa cctgcggccc acgacttaga aggtcgttgc
    12301 tctatccaac tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat
    12361 acggtcaacc cttgctgagt caatggcttt tgatctggtt gctgaacaag tgaacgaccg
    12421 cgtctgattt tctgatttat ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct
    12481 gaatcaagaa aacccgtatt ttcatgtatc aaagtacaat ttaccgccct aacggaaaat
    12541 tgtccgctcc tatgagactg gtaactatga aaccaacgtc ggtgatcatt atggatactc
    12601 atcctatcat cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg
    12661 tcctgaaaac ggatgattac cgcataacca tcgattatct ccgaacccgt cctgttgatt
    12721 taatcattat ggatatagac ttgcccggaa cagacggttt taccttcct
//
LOCUS       DAJTMX010000108        12661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTMX010000108 DAJTMX010000000
VERSION     DAJTMX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.108"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO145_003100"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO145_003100"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5468134.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO145_003101"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO145_003101"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5468135.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO145_003102"
     CDS             3115..3399
                     /locus_tag="OO145_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5468136.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO145_003103"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO145_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5468137.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO145_003104"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO145_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5468138.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO145_003105"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO145_003105"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5468139.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETT
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO145_003106"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO145_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5468140.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="OO145_003107"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="OO145_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5468141.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="OO145_003108"
     CDS             10849..11334
                     /locus_tag="OO145_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5468142.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="OO145_003109"
     CDS             11444..12433
                     /locus_tag="OO145_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5468143.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3341 a         3128 c         2940 g         3252 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc gtcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 c
//
LOCUS       DAJTMX010000109        12648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTMX010000109 DAJTMX010000000
VERSION     DAJTMX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.109"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            758..841
                     /locus_tag="OO145_003110"
     CDS             758..841
                     /locus_tag="OO145_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5468144.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO145_003111"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO145_003111"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5468145.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO145_003112"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO145_003112"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5468146.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO145_003113"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO145_003113"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5468147.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO145_003114"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO145_003114"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5468148.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHTSSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO145_003115"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO145_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO145_003116"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO145_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5468149.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO145_003117"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO145_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5468150.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO145_003118"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO145_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5468151.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO145_003119"
     CDS             10967..12166
                     /locus_tag="OO145_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5468152.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3113 a         3171 c         3358 g         3006 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacacct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DAJTMX010000110        12220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTMX010000110 DAJTMX010000000
VERSION     DAJTMX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.110"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..493)
                     /locus_tag="OO145_003120"
     CDS             complement(<1..493)
                     /locus_tag="OO145_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5468153.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO145_003121"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO145_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5468154.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO145_003122"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO145_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5468155.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OO145_003123"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OO145_003123"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5468156.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO145_003124"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO145_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5468157.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OO145_003125"
     CDS             3647..4156
                     /locus_tag="OO145_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5468158.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO145_003126"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO145_003126"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5468159.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OO145_003127"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OO145_003127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5468160.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OO145_003128"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OO145_003128"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5468161.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OO145_003129"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OO145_003129"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5468162.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OO145_003130"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OO145_003130"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5468163.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OO145_003131"
     CDS             complement(8798..9691)
                     /locus_tag="OO145_003131"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5468164.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OO145_003132"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OO145_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OO145_003133"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OO145_003133"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5468165.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="OO145_003134"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="OO145_003134"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5468166.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAJTMX010000111        12197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTMX010000111 DAJTMX010000000
VERSION     DAJTMX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.111"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            256..780
                     /locus_tag="OO145_003135"
     CDS             256..780
                     /locus_tag="OO145_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5468167.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="OO145_003136"
     CDS             861..1535
                     /locus_tag="OO145_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5468168.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4031
                     /locus_tag="OO145_003137"
     CDS             1551..4031
                     /locus_tag="OO145_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5468169.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            4047..5080
                     /locus_tag="OO145_003138"
                     /pseudo
     CDS             4047..5080
                     /locus_tag="OO145_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="OO145_003139"
     CDS             complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="OO145_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5468170.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5719..6549)
                     /gene="rcnA"
                     /locus_tag="OO145_003140"
                     /pseudo
     CDS             complement(5719..6549)
                     /gene="rcnA"
                     /locus_tag="OO145_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6671..6943
                     /gene="rcnR"
                     /locus_tag="OO145_003141"
     CDS             6671..6943
                     /gene="rcnR"
                     /locus_tag="OO145_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5468171.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7035..7131
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7166..7954
                     /gene="thiM"
                     /locus_tag="OO145_003142"
     CDS             7166..7954
                     /gene="thiM"
                     /locus_tag="OO145_003142"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5468172.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7951..8751
                     /gene="thiD"
                     /locus_tag="OO145_003143"
     CDS             7951..8751
                     /gene="thiD"
                     /locus_tag="OO145_003143"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5468173.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8816..9637
                     /locus_tag="OO145_003144"
     CDS             8816..9637
                     /locus_tag="OO145_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5468174.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9685..10431
                     /locus_tag="OO145_003145"
     CDS             9685..10431
                     /locus_tag="OO145_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5468175.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10405..11370)
                     /locus_tag="OO145_003146"
     CDS             complement(10405..11370)
                     /locus_tag="OO145_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5468176.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11367..12173)
                     /locus_tag="OO145_003147"
     CDS             complement(11367..12173)
                     /locus_tag="OO145_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5468177.1"
                     /translation="MCLADALLECEGKIDPNLIGRNILDWALRFDAFNKNVLGPTSKI
                     ALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALASSPTHKSDL
                     AVAGAVVIAWAISRAIDGERWSAIVDSLPSIARHAQQKRTTTFSASLAARLEIALKIV
                     RNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCANLGGDTDTI
                     GAMATAICGALHGINAVDPALKAELDAVNQLDFNRYATALAKYRQQREAV"
BASE COUNT         3286 a         2752 c         3033 g         3126 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggccgtag ccatattgaa ggggcgagca agcaaagtga ttttgtccag gcaggttatc
     2641 agtatggttt taataactta ttgacgctgt atggcggcac gatggttgct aataattact
     2701 atgcgtttac cctcggaacg ggttggaaca cgcgcattgg cgcaatttcc gtcgatgcca
     2761 cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt tatcaaattg
     2821 cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc tggcgttatt
     2881 cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa gataattatc
     2941 gccgtgatga aaacgatatc tatgacattg ccgattatta ccagaacgat tttggccgta
     3001 aaaatagctt ttctgccaat atgagccagt cattgccaga aggctggggt tctgtgtcct
     3061 taagtacgtt atggcgagat tactgggggc gtagcggcag cagtaaggat tatcagttga
     3121 gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag gcgtatggcg
     3181 agaatcatca tgaagagaaa cgttttaata tttttatatc aattccctgt gattggggtg
     3241 atgacgttac gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg tttgatgatc
     3301 agggttttgc ctcaaataat acgggattat caggaacagt agggagtcgg gatcagttca
     3361 attatggtgt caacctgagt catcaacatc agggaaatga aacgacagct ggggcgaatt
     3421 tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg agtacttatc
     3481 gacagactgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg
     3541 cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa gatgcttatg
     3601 tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtggtatac gacggaatga
     3661 caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat
     3721 tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg gttaattttg
     3781 ataccgatca gcgcaagccc tggtttataa aagcgttaag agcggatggt caaccattaa
     3841 cgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc ggccagggaa
     3901 gccagttatt tattcgcacc aatgaaatac cgccatcggt taatgtagca attgataagc
     3961 aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt agaaattata
     4021 tttgccagta atagtcaggt ggttctatgg aaattcgcat aatgctattt atattaatga
     4081 tgatggttat gcctgtgagc tatgcggcat gttatagtga gttatctgtt cagcacaatt
     4141 tggttgttca ggggaatttt gcacttactc aaacacaaat agcgacatat gagcataatt
     4201 ttaatgattc gtcatgcgtg agtacaaata ctatcacccc tatgagccca tcggatatta
     4261 ttgttggact ttataacgat accattaaat taaatttaca ttttgaatgg accaataaaa
     4321 acaacatcac gttgtcaaat aatcagagca gtttcaccag tggttattcg gtaacggtga
     4381 cacctgcggc cagtaatgca aaagtgaata tttctgcggg gggcggtggt tcagtgatga
     4441 ttaatggtgt tgcgacatta tccagtgctt catcatcgac acgcgggagt gccgcagtac
     4501 aatttctact gtgtttatta ggtggcaagt catggggtgc atgtgtaaat agctacagaa
     4561 atgcattggc acaaaatgca ggtgtctatt ccttcaatct gacattgtca tacaacccga
     4621 taaccacaac ctgcaaacag gacgatttat taattacttt agacagtatt cccgttttac
     4681 aattaccagc cacaggtaac aaagcaacaa taaatagtaa aaaaagggat attattctgc
     4741 gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag gtgtatttat
     4801 caagttctga cttgttaacc aacagcaaca caatactgaa aggtgcggaa gataatgcgt
     4861 aggatttatt cttgaaagta atggttcgcc agtcacactt ttaaatatca ctaacagcag
     4921 taaaggatac acaaatttaa aagaaattgc ggcaaagtca aaacttacag atacaacggt
     4981 ttcaattccg ataacagcca gttactacgt ctacgataca aacaaagtta aatctggcgc
     5041 actggaggca accgcattaa tcaacgtgaa atacgactaa taacaagcaa aacgagcaag
     5101 tggctaataa taaaaaataa caaggtgtct gcatttcccc ctcatgatga gggggctttt
     5161 tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc
     5221 tgatcaacac gtacgcgcca cccatatagg tccagtgcgt cccggcatca ggcgcgggca
     5281 ggttacgtaa ctgccactgc ttaatgttgt actcatcggt gcggtacatc tctgggacgg
     5341 tatcacctgg tttgaaatgc ttaaagtcag caatgaaact acttaactca tacttagaaa
     5401 ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca
     5461 gcaaaagcac acccagcaac atcttatttt taatggtcat aaactctcca ctttggggat
     5521 ctgtaatatc agtcgcatat ctttcctgtc atacaatata actgacaagc gatattttgt
     5581 tagtgatccc atgatttcgt aaaaagtgta aatgaaagga tgccctaaaa gcatccttag
     5641 cgagcagaga gtcagcggaa atagctgatt taagaatcat cctgggctga agataacatg
     5701 caagcctaaa ggattgagtt atcgcattat gcccatgaag ccatgtacgc ccatatacac
     5761 accgactaag ccaatcaaca gactggaaaa atagggggcg cgtttagcga gagtgttaaa
     5821 tccgctccag cgttttgcga cctgctgaac gctgattgct gcgccaacgc ctacggtgac
     5881 aagcgttaac gccaggccaa tgctgaaact gacgaccagt gttgcgccca gtgtcagggc
     5941 tttcaactga atgcatatca acagcacggt aattgctgcc gggcagggga taaagccacc
     6001 ggttaagcca aataacaaaa tttgccagtt gatgacctct ctaccatcaa agcgtcgttt
     6061 aatgtcattg gcatgggctc gtgcatgggc atcctgatac tcgccatgtt cgtgatggtg
     6121 atgatgtcct tggtcgtggt gatgttcgtg atcgtgatga tggtgatgat gctcataatc
     6181 atgcccgtgc atattctcca gccagttgcg ttcgccgcgc taggtacgcc agaacatcca
     6241 gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt ctgctgattg
     6301 agcagtaaag cgcttactga tcaccatccc gccaaaggca atcaaccaga ccaccgcggt
     6361 atgcgaaatg gttgctgcca gtccgagcat caccgcctgt ttaatggtgc ctttgatggc
     6421 gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat gaagcgcacc
     6481 aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa gagttgtaaa
     6541 ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact cccccccagt
     6601 acctgatact acccccccag tagattaata gtgctatgat ttttcatatt cttgttaacc
     6661 aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc gtgccagtaa
     6721 gattcagggc caggtcgtgg cgctcaagaa aatgctcgac gagccgcacg aatgcgctgc
     6781 agttttacaa cagattgctg ctatccgtgg agcggtaaac ggtctgatgc gggaagtgat
     6841 taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac gtgaagaaga
     6901 tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta acgcgattgt
     6961 aaaactgccg tttttcctcg tttacaacgg gggcgctgga cattaccatc ctcctctgcg
     7021 atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg agaaataccc
     7081 gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag gtcattgctt
     7141 cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgagttcag cgcaatctgc
     7201 gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga ccaatgatgt
     7261 ggtgcaaacc tttaccgcca ataccttgct ggcgctcggt gcatcgccag cgatggttat
     7321 cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga ttaacgttgg
     7381 cacactgacg cagccacgcg cacaggcgat gcgtgctgcc gttgagcaag caaaaagctc
     7441 tcaaacacgc tggacgcttg atccagtagc ggttggtgcg ctcgattatc gccgccattt
     7501 ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt cggaaatcat
     7561 ggcattagct ggcattgcta atggcggacg gggagtggat accactgacg ccgcagctaa
     7621 cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg tggtcactgg
     7681 cgaggtggat tatgttaccg atggacatcg tatcattggt attcacggtg gcgatccgtt
     7741 aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg cctgctgtgc
     7801 gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga aacaagccgg
     7861 agaacgcgca gtcgccagaa gtgaggggcc aggcagtttt gttccacatt tccttgatgc
     7921 gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct gacgattgcc
     7981 ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt ctcggcactt
     8041 ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg tggcgtacag
     8101 tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt gttcagcgat
     8161 gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt tgaagcggtg
     8221 gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt tatgctggca
     8281 aaaagcggcg acccgctgct ttcaccttcg gcggttgcta cgctgcgcag tcgattattg
     8341 ccacaggttt cattaataac gccaaacttg cccgaagctg ccgccttgct cgacgcgcca
     8401 cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc gatgggctgt
     8461 ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga ctggctgttt
     8521 acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa cacccacggc
     8581 actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac aaactgggct
     8641 gacaccgtac aggaggcaaa aatctggctt tcatcggcgt tagcccaggc cgacacgctg
     8701 gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg agcaccgtac
     8761 tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga aaccaatgca
     8821 gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg ggctaatttc
     8881 catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag cagttcagat
     8941 tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta ttcgcattcc
     9001 tgccagttac accattcacg gattagatgt ttcgcgctgg caggagcgta tcgactggca
     9061 gcgggtggca aaaatgcgcg acaacgatat ccgcttacag tttgctttta ttaaggctac
     9121 ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaattaa gccgcgaaaa
     9181 tggcctgctg cgcggggcgt atcattattt ctccccgtcg gtatccgctt cagttcaggc
     9241 gcgattattt ctgcaaacgg tggatttctc acaaggcgat ttccctgccg tgctggacgt
     9301 agaagaacgg ggaaaattat cggcaaaaga attacgcaag cgggtaagtc agtggctaaa
     9361 aatggtcgaa aaacgcacgg gaaaaaagcc gattatttac tcaggagccg ttttttatca
     9421 caccaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact attatcaacg
     9481 tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc gtggacaggt
     9541 agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacgggga tgagttgcag
     9601 gcattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat actcccacca
     9661 gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat tgcccaactg
     9721 aacgagcgta ttttagcggc ggataacacg ccgctttata ttaagtttgc cgaaacggta
     9781 aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg tgagcgtgat
     9841 ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca ggcgctggaa
     9901 gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa catcttcgaa
     9961 tactcgttga aagaagcgag aggtttttct cagcaggtgg tattgcgcgg gaaaaagccc
    10021 gatacgctat gggttaataa gcgtgtcgtg aaatgtcccg aagaagttgc gcagcagctg
    10081 gcggtcgaag cgggaagtga tgtctttttg cttaagcgta ttcgctatgt cgatgaagaa
    10141 gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt tgatgccatc
    10201 ggaatttctc tttatgacta tttccgcagc cagcacattt acccacaacg tacgcgttcc
    10261 cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt agatagcaaa
    10321 ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc tattgagtac
    10381 agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta gttcctcgcg
    10441 cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg cattgcccag
    10501 taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc cacctgcatg
    10561 actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa tgcagccaga
    10621 agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca acggcgcggc
    10681 aaatttctcc tgccattgct tgccaagtgt tgttatctct gcggataaag caaaacgttc
    10741 ggcggcaatc tcagcctctt gacgattgag tgacactaaa ggtcgacagg ccatgatccg
    10801 cgccagtaat gcgtccggga tatcgccaat acgtgggcaa aaatcgataa acggcgtcac
    10861 gtcttgcagc ttttccagcc attccactaa caattcgccg cagggcgagg ccagttgata
    10921 accggaaaaa tagagcaggt tgccaggcgc aacggttaat cgtgccaacc attggcgatt
    10981 ccattgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg gctcaaccag
    11041 tgccagacac cagccgttat cgctttcggc gttatcgatc aggctgatta acccctcttt
    11101 cgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca aggcattacc
    11161 cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc cgccaacgtt
    11221 aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat aagcgtcggc
    11281 gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg ttaattcagg
    11341 cagcagtgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg atatttcgcc
    11401 agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagctc cgcctttaac
    11461 gcaggatcga cagcgttaat gccatgcaac gcgccgcaaa tcgccgtcgc catagcacct
    11521 atggtgtctg tgtcgccgcc aagattggcg cacaggacgg cgcagcggtt tgggtcggtt
    11581 tgtgccagtt caactagcgc aatggcgcac ggaacggact caatagtgct ggtgcctgcg
    11641 ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt acgcacaatt
    11701 ttcagcgcaa tctccagacg tgccgccagt gaggcgctga acgtggtggt gcgtttttgc
    11761 tgtgcatgtc gcgcaattga aggcaatgaa tcaacaatcg ctgaccagcg ttctccgtca
    11821 atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag atcggattta
    11881 tgggtcgggc tggacgccag cgccacatca tcaataaagg aatcaacatc acgcgctgga
    11941 agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggttacgcc gttgttttcc
    12001 agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct
    12061 agtacgtttt tgttaaaggc gtcgaaacgc aacgcccagt cgagaatatt acgcccaatc
    12121 agattcggat cgatcttgcc ttcacattcc agtaacgcat ccgccagaca caggccatcg
    12181 aggtatcgtc ggtgaat
//
LOCUS       DAJTMX010000112        12165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTMX010000112 DAJTMX010000000
VERSION     DAJTMX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.112"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO145_003148"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO145_003148"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5468178.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO145_003149"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO145_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5468179.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO145_003150"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO145_003150"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5468180.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="OO145_003151"
     CDS             4129..4884
                     /locus_tag="OO145_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5468181.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="OO145_003152"
     tRNA            4963..5036
                     /locus_tag="OO145_003152"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="OO145_003153"
     CDS             5171..6781
                     /locus_tag="OO145_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5468182.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6813..7420
                     /locus_tag="OO145_003154"
                     /pseudo
     CDS             6813..7420
                     /locus_tag="OO145_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8351..9097
                     /locus_tag="OO145_003155"
     CDS             8351..9097
                     /locus_tag="OO145_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5468183.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9395..9886
                     /locus_tag="OO145_003156"
     CDS             9395..9886
                     /locus_tag="OO145_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468184.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            9970..10959
                     /locus_tag="OO145_003157"
     CDS             9970..10959
                     /locus_tag="OO145_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468185.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            11621..>12165
                     /locus_tag="OO145_003158"
     CDS             11621..>12165
                     /locus_tag="OO145_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468186.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
BASE COUNT         3134 a         2995 c         3034 g         3002 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta
     7621 agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc
     7681 tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc
     7741 catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt
     7801 tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat
     7861 cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa
     7921 ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt
     7981 cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat
     8041 ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta
     8101 gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact
     8161 taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc
     8221 agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc
     8281 cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga
     8341 gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat
     8401 gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat
     8461 tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag
     8521 cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg
     8581 tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta
     8641 ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct
     8701 tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac
     8761 ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct
     8821 ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa
     8881 gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta
     8941 taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa
     9001 gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct
     9061 gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac
     9121 ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc
     9181 ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc
     9241 aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt
     9301 taaacacctt ttgagatgac cgatgcgggg agacccgtag gccttactgt gcctgcgata
     9361 ccgatagtct caactctcaa gaggagaatt acctatgatg ttcaacaaca acaactggaa
     9421 gctgtcagtc accgacatca acctttatga aaacactgtg agtctggacg gtcagtccta
     9481 tccgttgtcg ctcgctatca agaccctcat tccgggctac ctgtcaggct taccgtccac
     9541 cagccgcgag gcgatggagt tactggaagc tctggctgaa gctggcgtga ccattggtaa
     9601 cttcttcagt aatgacctga tgaccgccta ccaacgccgc cagatgaaca aacgagctga
     9661 agctgagcgt atcgctaaag agctagcgtc tcagaaagag cgtacccgcg aaatgttcat
     9721 gaccgaagac gaatggcaaa aagagctaca gcgccgcgaa caggtcaaag ctgaacgccg
     9781 gacctatggt gaaaacctcc gcagcgctac tcattccgct ggtcgctcac gtgccgctat
     9841 cgtcgctgac cttgagtcag gcggtaactg gatggacagc ttgtaagcaa gcgtccctca
     9901 tctgagaaca cacggtccct gactgcctgt tagggacttc aagtatttga ctcaaggaga
     9961 ttaattataa tgaatatgac taaaaacgct ttccagtttg gcattgagcc agtacgcatc
    10021 accgataccg acaacattca ggtcaatgaa ggtctgccga ctaacgccga ccctcaggtc
    10081 tatgccctcc agttagctaa gactgttaag gctatgctga acggtgtact taaagacgct
    10141 caagataaca tcccgttccc tgtagaagtc ctgccgaccc gtaacagctt accgactcca
    10201 atcattgctc acactctggc tgaccgctct gtgctggtcc ctgtccgtgg tggtaagcga
    10261 cctgaagttg tgaccgctcc gtctggcact gaaatcaccg ttgagccgat tgaacaggcg
    10321 atcctcgtgt ctcaccaaac aaagctttgg gaccagaagt cgaccactgg tttcactcag
    10381 gggacgctcc agcaggacgc actgaacatc tgcgataacg tgattcgtac cattaactct
    10441 aagatggttg acgtgctgga gtcctcaaag ctgctgaaga ccgtagaact tccggccctg
    10501 actggtagcc tgaccgctaa ggctgacgct atcatggatg ccctgtacga gaacacggag
    10561 tcttctttcg gttctgaggt ttctgactac ggcattatcg cccatgagtc acacctcaag
    10621 gctctctcac gtctggctgc taagcagggc ttcggtggtg aagacgctat cgtcgatatg
    10681 ctggggactg acgtcgccta ttacaacggt gaagaccgtg gcgtgttcat gatggctaag
    10741 cgttttacgg ctctgagctt cggttgcttc cgtcacgatg gcgagagcat tactgtggtc
    10801 ctttcacgtg atggagactc ccagtctcac gaccttgaga ttctcggcaa ggtgtttgtt
    10861 gtagctgaag ctgctacgac catcaagatg ggtactggct cggctactgc tgtcctgcct
    10921 gtagtcaaac gcctgagctt cactaaagaa gctaactaac aataacgact attggtcaga
    10981 cttccgaact ggatgtctgg cctttatttt gtactagtaa tctagtttgt gaatacagaa
    11041 agtaggaggg ccaaatggac ctacacaaag tctatctcgt ggtcttcctg ctaaagctgg
    11101 gactggtggt ctggctatgg gctattagac gatgagtttt gactgaaaaa tgtgagacct
    11161 catctaatat aaagagcgag ccaaaagccc ccatagcctg acctgagcca gaccaaaagg
    11221 gccgggtagt cagaccgtga gttctattcc tttagaggga atattacgtg cttcaagtct
    11281 tgaccttcta tctctaaatg tgtgactatc actacaacca actggattca cctgtcttta
    11341 agaaacaata ggttagctgg ttgtcttcga tttgtcgtag gctcccttcg cccgctccaa
    11401 tttatcttca cctgatcaat ctgccatttt tgctgtatct gattttctat attatcttaa
    11461 ttcaatcact tcaggctttt cgcaatcgct gtaacacttc cccatttttt tgtaaataaa
    11521 aagcgtttac gcaatgcaat ccttttgtaa caactcaggt aaaatacctt attttcaagt
    11581 tgttgaataa gtggttatct acgcttttaa caaacgattg atggattatt ttatgaaagg
    11641 gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac
    11701 agaggcagaa gtgacaagcg aatctgtatt taacattctt aattccaggg gggcagcaac
    11761 agataaaagt tatttatcgt taaatccaga taagtatcct aattatcgtc ttctgattca
    11821 ctcagccaaa ttaaaaaatg aaataaagag tcactatacc aaagacgaaa ctcaaggatt
    11881 gctaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacgtt
    11941 tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt
    12001 ctggctgagg gatagtttat ggggctatat ggcattagta tcagaccaag gaaatagtgt
    12061 agccgcaaaa aaggttctgc tcaccctgtg gggttatatg tctacgcccg accagattaa
    12121 gcggatgcag gacatcatta gcaacccaaa acgactggat ggaat
//
LOCUS       DAJTMX010000113        12113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTMX010000113 DAJTMX010000000
VERSION     DAJTMX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.113"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..371
                     /locus_tag="OO145_003159"
     CDS             <1..371
                     /locus_tag="OO145_003159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468187.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="OO145_003160"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="OO145_003160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5468188.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="OO145_003161"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="OO145_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5468189.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="OO145_003162"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="OO145_003162"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5468190.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="OO145_003163"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="OO145_003163"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5468191.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="OO145_003164"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="OO145_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5468192.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="OO145_003165"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="OO145_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5468193.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="OO145_003166"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="OO145_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5468194.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="OO145_003167"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="OO145_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5468195.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="OO145_003168"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="OO145_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5468196.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="OO145_003169"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="OO145_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5468197.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="OO145_003170"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="OO145_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5468198.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12113)
                     /locus_tag="OO145_003171"
     CDS             complement(11913..>12113)
                     /locus_tag="OO145_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5468199.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3388 c         2934 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aag
//
LOCUS       DAJTMX010000114        12007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTMX010000114 DAJTMX010000000
VERSION     DAJTMX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.114"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            110..1009
                     /gene="lysO"
                     /locus_tag="OO145_003172"
     CDS             110..1009
                     /gene="lysO"
                     /locus_tag="OO145_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5468200.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1153..2805
                     /gene="hcp"
                     /locus_tag="OO145_003173"
                     /gene_synonym="priS"
     CDS             1153..2805
                     /gene="hcp"
                     /locus_tag="OO145_003173"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5468201.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2817..3785
                     /gene="hcr"
                     /locus_tag="OO145_003174"
     CDS             2817..3785
                     /gene="hcr"
                     /locus_tag="OO145_003174"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5468202.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3918..5636
                     /gene="poxB"
                     /locus_tag="OO145_003175"
     CDS             3918..5636
                     /gene="poxB"
                     /locus_tag="OO145_003175"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5468203.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5673..6674
                     /gene="ltaE"
                     /locus_tag="OO145_003176"
     CDS             5673..6674
                     /gene="ltaE"
                     /locus_tag="OO145_003176"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5468204.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6685..8115
                     /locus_tag="OO145_003177"
     CDS             6685..8115
                     /locus_tag="OO145_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5468205.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8214..9227
                     /locus_tag="OO145_003178"
     CDS             8214..9227
                     /locus_tag="OO145_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5468206.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="OO145_003179"
     CDS             complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="OO145_003179"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5468207.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10051..10374)
                     /locus_tag="OO145_003180"
     CDS             complement(10051..10374)
                     /locus_tag="OO145_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5468208.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10585..>12007)
                     /locus_tag="OO145_003181"
     CDS             complement(10585..>12007)
                     /locus_tag="OO145_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5468209.1"
                     /translation="IRNVSNGKLERQQFLFPLDYENKLQEQLDKYFMIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2860 a         3090 c         3028 g         3029 t
ORIGIN      
        1 cccctctgat gcataacatt tccttttaat tgcaacttta ctcgcccttc cgctttcagt
       61 agtgtgtcat cgcgcatttc gacttcttct ttgcaaggac tggattttca tgttttctgg
      121 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
      181 tgcgttaaaa gctattaatc agctattaag ctggatggtt taccttattc tcttttttat
      241 gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta
      301 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
      361 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
      421 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
      481 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
      541 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
      601 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
      661 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
      721 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
      781 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
      841 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
      901 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
      961 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
     1021 cggtagttcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
     1081 tcccttttat ccccgcgtta agcgtcttac ccttaaacat gtatattaaa tataacttta
     1141 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcagaaaac
     1201 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
     1261 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
     1321 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
     1381 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
     1441 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
     1501 gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
     1561 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
     1621 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
     1681 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
     1741 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
     1801 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
     1861 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
     1921 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
     1981 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
     2041 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
     2101 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     2161 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     2221 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     2281 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     2341 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     2401 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     2461 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     2521 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     2581 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt
     2641 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     2701 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct
     2761 atcaccactg ttgaagaaga catgaagcaa ctgttgaacg cgtaaggagt ttgtcgatga
     2821 cgatgccaac gaatcaatgc ccgtggcgga tacaggttca tcacattacg caagaaacgc
     2881 cggatgtgtg gacgatttcc ctgatttgcc acgatcacta cccatatcgc gccgggcaat
     2941 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     3001 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     3061 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     3121 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     3181 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     3241 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     3301 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgcc
     3361 tcactcgcga actgctggca gatgtacctg acttagcttc acgtaccgtg atgacctgcg
     3421 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     3481 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     3541 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     3601 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     3661 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     3721 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     3781 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     3841 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     3901 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     3961 cggcaggcgt gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagcgaca
     4021 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcgt
     4081 ttgccgctgg cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg
     4141 gcccaggcaa cctgcacttg atcaacggcc tattcgattg ccaccgcaat aacgttccgg
     4201 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     4261 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     4321 agcagatccc acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt
     4381 ccgttgttgt attaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     4441 actggtatca tgcgccacag ccagtcgtga tgccggaaga agaagagtta cgcaaactgg
     4501 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     4561 cgcataaaga gttagttgag tttgccggga aaattaaagc acctattgtt catgccctac
     4621 gcggtaaaga gcatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     4681 gattctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     4741 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatattaatc
     4801 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     4861 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggataaag
     4921 cgctggaaga ttaccgcgat gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
     4981 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca
     5041 tctttacctg tgacgtcggc acgccaacgg tatgggcggc acgttatctg aaaatgaacg
     5101 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
     5161 cgttgggtgc gcaggcaacc gagccggaac gtcaggtggt cgccatgtgc ggcgatggcg
     5221 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccggtgaaaa
     5281 ttatcgtctt taataacagc gtgctgggct ttgtggcgat ggagatgaag gccggaggtt
     5341 acctgacaga cggtactgag ctgcacgaca ccaactttgc ccgcattgcc gaagcgtgcg
     5401 gcattacggg tatccgtgta gaaaaagcct ctgaagttga tgaagccctg caacgcgcct
     5461 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagaa ttagccattc
     5521 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
     5581 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
     5641 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
     5701 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
     5761 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
     5821 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
     5881 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
     5941 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
     6001 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
     6061 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
     6121 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
     6181 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
     6241 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
     6301 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
     6361 agtccgggat tctggctgcc gccgggatgt atgccctgga aaataacgtc gcacggttgc
     6421 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
     6481 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
     6541 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
     6601 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
     6661 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
     6721 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctagcggcg
     6781 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
     6841 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
     6901 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca gctggctctc
     6961 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
     7021 caggccccgc cacatgagca gtcggatcat ctgcgagctc gtcaggctac ggcggacatt
     7081 cttcgtgaag cgaatgtacc agtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
     7141 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
     7201 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
     7261 gcgctgttag accatccagc caacgaacac cgcatcttcg aagccgccgg accagaggtg
     7321 ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg
     7381 atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc
     7441 gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct gctggcagat
     7501 gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga cgcggtacgt
     7561 agcacgttga aagaggaaga aaaactggtc aactccagcg actggggcta cgacgctcag
     7621 gcctttgccc gctggcgacc ggagtacggt tatttcgcca aacaggcggg gtttaccgtt
     7681 aaaacgtccg ccagccttgc ggctttatgg ctggtggtga accaaatcgg cggtaaagag
     7741 cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg cgcgatcggt
     7801 cataatctgg cgaaaggccg cccggagcgc gaatatttac agaccggcga tgcggtggat
     7861 agctggaaag tgattgtcgt tgaaccgaaa aagcaactta cgttgttatt tggcatgaaa
     7921 gcgccggggc tgggacgact gtgttttacc ctggaagata aaggtgacta tcgtactatc
     7981 gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg
     8041 atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag actggcagaa
     8101 caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg
     8161 ggggaaatca cggaagaatg cgcacgtcat tttctcaaca acagtggatt gatatgaagg
     8221 tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga
     8281 aaggcatcag cgtgcgtgcg agcggtcgca acgaagcaat gggtaaattg ctggaaaaaa
     8341 tgggcgcaga gtttgttccg acggatctga ccgagctggt ttcatcacaa gctaaagtga
     8401 tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc tgggggacac
     8461 aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggctgtcg
     8521 cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc
     8581 accatcgcaa tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca
     8641 gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct
     8701 ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt attccccgtc
     8761 tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc agtgcgctgg
     8821 tcgatatgac ctactatgaa aatgccgtgc acgcaatgtg gctggcaagc caggaagcct
     8881 gcgataagct accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc
     8941 gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc
     9001 cttacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa
     9061 aagagccgcc gctgacccac tacggcgcct ccaagttgaa ttttgacttt acgctggata
     9121 ttacgcgtgc gcaggaagag ttaggttatc agccggtcat caccctggat gaaggcatcg
     9181 agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt
     9241 tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg
     9301 tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc ggtgtcatat
     9361 caggttttac gtcataaccg tagcgcgcca aaagctccag caatgacgcg gtatctaccg
     9421 gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttcacccg ctgcgcatcc ggccaggcac
     9481 caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct
     9541 gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa
     9601 tatctttcgc cagcggaata agcgcctgaa tctgtgcctg ttcaaacggg gcaaaatatt
     9661 tcactccgga tgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt
     9721 taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg
     9781 gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggcaccgca gggatcagat
     9841 aatgcgagct gacctgctta tcagtcagtg tcgccagcga gctatcaaaa tcatctgcgg
     9901 tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg tgtcgggtat
     9961 caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca
    10021 gagcggcagc gaccagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct
    10081 gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc
    10141 aactacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa aggcgatctc
    10201 ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga tatcgcggat
    10261 accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc
    10321 gcaatattca acgatggtca ggccttccag agttggggtt gtagaaaatt gcatgatcga
    10381 tctccttgtt tgttaagcct tcgatctcta aagcattatc ggactgatat gccatgttgg
    10441 gaagaaggtt ttgatcagtt taggaaatca tctcattgtg tcaaaaacac ccacttaagt
    10501 ctttacgatt atccgttgca taacccgata ttggtttcat tatagggtac cgcttacctg
    10561 tcttattatg ttgcaccaac aacattaatg agcggaaaga ggcaacttat attgtaaatg
    10621 actcctaaag tcttccccat cttgtgactg aggtctatac cgctggattg ctttttggtc
    10681 agccgccgca aaacaagaaa gtaataagat ctcaatgctt ttgtcattaa tataagcgaa
    10741 gatcattctt gcaaactcgt atataaacgc ccgatcctga gcgcttaata gtttaataca
    10801 atatttattt ttaagagaga aaataatttc aaggaaagca tccgcagcaa ccttcacgtt
    10861 attcccggcg ttcatattat atatatccct tgcacagata gagtacagag atagtaaaca
    10921 gccatacaaa taatcactta ttaattcttt tgtccatttt acaggcaacc agggttttat
    10981 cttgacacca tgcatactac attcatgaaa aagacatttg ctgagattaa ctcttatcat
    11041 atcaacattg aaaaaattgc agttaataaa ttttaaagaa tgagtattta cgttattaat
    11101 tttaacagat ctaaacgaaa tatttttgaa tgcactgttt attacattgc tatttgttat
    11161 tttaacatca cctaaactgc cgttttcaaa acaacatttt ttcaatatgc tttcattcac
    11221 aacagtgtgt gataaataga gattacttat gttttgcttt attaatgcca cgtcattaaa
    11281 atgactatat ttaatgaaac aaccatcatt gagtagtgtt ttaagattga ctaaattact
    11341 tgcatcttca cgtaccatcg tggcaactgg aacaggcaga gacatgaaaa cttcttcatt
    11401 caccatcaag tgaatcatgc attgcgttcc ctttgtcata tccacactta cgctacaaat
    11461 atcgtggtaa tgcgaagagt gcatttcagg aaacagatta aacacggtat cttgaaaggc
    11521 actaaatcgt gttaataaat cctgataatg aacaggtttt gtttcaaaat tatcatcagc
    11581 aaatccaagc actattgcat gctctacatc taacgcagtt tcaaatgact cttcttctgt
    11641 aaaatcatta tttaaaacct cacctgacgt attatcactg tcataaaaag tgctcatcaa
    11701 tgcgatattg tttgttacag cgtctttatc gatattctca ctaatagcat atcgcctgct
    11761 ataatcataa aaatgaataa atgcgtcctt tataaaggat atatattgtt ctttgttaaa
    11821 gtcaatacga ctgtataact tttcaaaaat cacattcgca atcttactac caatggtata
    11881 cctgtataat aatgggtgcg attcaatcat aaaatactta tctaattgtt cttgtaattt
    11941 attctcatag tccagtggga aaagaaactg ttgtctttca agttttccat tcgatacatt
    12001 acgtatt
//
LOCUS       DAJTMX010000115        11882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTMX010000115 DAJTMX010000000
VERSION     DAJTMX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.115"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..336)
                     /locus_tag="OO145_003182"
     CDS             complement(<1..336)
                     /locus_tag="OO145_003182"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5468210.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVS"
     gene            complement(469..774)
                     /locus_tag="OO145_003183"
                     /pseudo
     CDS             complement(469..774)
                     /locus_tag="OO145_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            842..1457
                     /gene="tnpA"
                     /locus_tag="OO145_003184"
                     /pseudo
     CDS             842..1457
                     /gene="tnpA"
                     /locus_tag="OO145_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1785..2867
                     /locus_tag="OO145_003185"
     CDS             1785..2867
                     /locus_tag="OO145_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5468211.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3356..4154)
                     /locus_tag="OO145_003186"
                     /pseudo
     CDS             complement(3356..4154)
                     /locus_tag="OO145_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4164..4715)
                     /gene="ybcL"
                     /locus_tag="OO145_003187"
     CDS             complement(4164..4715)
                     /gene="ybcL"
                     /locus_tag="OO145_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5468212.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4884..5216)
                     /gene="emrE"
                     /locus_tag="OO145_003188"
     CDS             complement(4884..5216)
                     /gene="emrE"
                     /locus_tag="OO145_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5468213.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5561..5875)
                     /gene="fliE"
                     /locus_tag="OO145_003189"
     CDS             complement(5561..5875)
                     /gene="fliE"
                     /locus_tag="OO145_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5468214.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6089..7748
                     /gene="fliF"
                     /locus_tag="OO145_003190"
                     /pseudo
     CDS             6089..7748
                     /gene="fliF"
                     /locus_tag="OO145_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7741..8736
                     /gene="fliG"
                     /locus_tag="OO145_003191"
     CDS             7741..8736
                     /gene="fliG"
                     /locus_tag="OO145_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5468215.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8729..9415
                     /gene="fliH"
                     /locus_tag="OO145_003192"
     CDS             8729..9415
                     /gene="fliH"
                     /locus_tag="OO145_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5468216.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9415..10788
                     /gene="fliI"
                     /locus_tag="OO145_003193"
     CDS             9415..10788
                     /gene="fliI"
                     /locus_tag="OO145_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5468217.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10810..11151
                     /gene="fliJ"
                     /locus_tag="OO145_003194"
                     /pseudo
     CDS             10810..11151
                     /gene="fliJ"
                     /locus_tag="OO145_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11148..>11882
                     /locus_tag="OO145_003195"
     CDS             11148..>11882
                     /locus_tag="OO145_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HCR5468218.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLS"
BASE COUNT         3167 a         2930 c         2898 g         2887 t
ORIGIN      
        1 gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg
       61 cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct
      121 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggctc
      181 aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca gcccgaccat
      241 tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac tgatgtacca
      301 cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctgtt
      361 ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct gagcgagctt
      421 aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc aggggaacgc
      481 cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg actgttgcaa
      541 tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc actcaaccaa
      601 tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat tctcattctg
      661 acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg
      721 cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt tcacagggca
      781 aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact gtgttttcct
      841 gatgcatgct caccgggtct ttgttaccaa gtaccgacgc cagatttttg accatgacgc
      901 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
      961 tgaaatggat ggcgaaccag atcacgtcca tctgttaatt aactatcccc ccaaactggc
     1021 gatatccagt ctggtaaaca aacagcctca aaggcgtatc gggtaggtta ctgcgacggg
     1081 atcgaccaga tattgctgcc aggtattacc gcaaagatgt tttgtggcgt cccggctatt
     1141 ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt gaacaacagc
     1201 aaacacctgg tcagatgaaa aaacgagcct tatatccccg ccctgaagga cgggctttta
     1261 cggtgcactg gataagcctg gagattatct acacctgcat ggacatcatt gtactatgcc
     1321 ccgtacatgc tccatcacat cctcaacatc aaagtattag agccttgcaa accattatgc
     1381 gtctcagttt gtctcatcta tcttacgcaa aaaaaataga actaatatca agcggcgcta
     1441 tattgcgtat taaataaaac cacctcaatt acattaataa atctaaataa atatcatgca
     1501 aataatttcc atggttattt ttaggctatt taaattaaaa aaataaatta tgagtcaatc
     1561 ataaataaca acctttaata taatttgact accaaattta tattcgttca caaaatagtc
     1621 atgcattgtg tgcaatagaa acagttcaga taaaaatagg gatctacttc acaatttaaa
     1681 tgagaaacta aaacttacat cttgaaataa tctcattgat tggatgaata tttgtagtgc
     1741 agtgacatca tttttaaata atagttcaaa taaaagggct cacgatgaaa aaattaacag
     1801 tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag gcagcagaga
     1861 tttacaataa agacagtaac aagctggatc tgtacggaaa agttaatgct aagcactact
     1921 tctcttctaa cgatgcagat gatggcgaca caacttatgt tcgtctgggc ttcaaaggcg
     1981 aaactcaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca
     2041 accgtgctga atctcaaggt tcttccaaag ataaaacccg tcttgccttc gctggcctga
     2101 aattcggtga ctacggctcc atcgattatg gccgtaacta cggtgtagca tacgatatcg
     2161 gtgcatggac cgacgttctg ccagaattcg gtggcgatac ctggacccaa acagatgtgt
     2221 tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa caacgacttc tttggtctgg
     2281 ttgatggtct gaactttgct gctcagtatc agggtaaaaa tgaccgcact gacgtaactg
     2341 aagctaacgg tgatggtttc ggtttctcca ctacttatga gtatgaagga ttcggtgtag
     2401 gtgcaaccta tgctaaatct gaccgcacta atgatcaggt tatctacggt aacaacagcc
     2461 tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat gatgcgaaca
     2521 acatctatct ggctaccacc tattctgaaa cccagaacat gactgttttt ggtaataacc
     2581 atattgccaa caaagcacaa aacttcgaag tagttgcaca atatcagttc gacttcggtc
     2641 tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg tggggtgatc
     2701 aggacctgat tgaatatatt gatgtaggtg caacctatta cttcaacaaa aatatgtcca
     2761 cttttgttga ttacaaaatc aacctgattg ataagagcga tttcacgaaa gcatctggcg
     2821 ttgctaccga tgatatcgtt gctgtaggat tggtttacca gttctaatct gattactcaa
     2881 agatatgttg tgggaggctt tgcctcccca acatataagt ggcgctctca agccactttc
     2941 cttagaagca ctatcttgct tctgactata taaattaccc tttattttct gggacgtttc
     3001 aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga tttaataaca
     3061 acgatatccg tacctaacct gatatctaat gtcataagca tccctttaat tatcccctca
     3121 acctcttgta aatttcccac tcttgagctc cacatagccc aaacgccgga tgcaaaaaaa
     3181 atcatacgtg catctctgaa attgtgatgg aggaagtgag tatcaattat atgtgcttat
     3241 aaacgttccc gattggcaca gaagtcaccg gattgttcag gctccaatag gttgagtatg
     3301 tcgtatttat tttccaaaat aaaaatcgat gctgttgctc cttaataaaa tacttttact
     3361 ctctggacgc ttttcttagc tctcttggtg ttatattaaa acgattatgg aacctttcag
     3421 taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg ggttttactg
     3481 tcgtttgtat agcaggacaa cgcattattt aacctcacgt cgtccaatat actttgaaaa
     3541 cttattccct cgcttgccag acggcgatgc aatgtggaaa cagaaatgta gagatatctc
     3601 gctaccttgt ttgctgtcca ttttgttcta ggttcggata gcattaggtt ataacaacga
     3661 cttattaatg attgtttact atctgataaa agtaaacaat taacatgatg tgctcctaat
     3721 gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc tcgggaaata
     3781 gatgttttta gttgctccca acaatatatt aactcggggt tctgaggtaa gcagaaacct
     3841 gtcgtgttac gaatttgatc agttatcgca taaaattttt gaaacttttc aattagatca
     3901 atgggataat aaagcatttc tgcaagataa agacctgctt ctggataatt cacaatataa
     3961 aattcatatc cacagggaaa taatattatc tgattattat caacagttag actatgcgtc
     4021 tcccaattga taactttttt tccctgacgg atacgacata aagctggcat aagaggctta
     4081 accctgtgaa tctcatgatg tttatgcatc cgtatttctt caatctttag gttagactta
     4141 tctcttgcca gcatactctt accctatttt atctcataaa ctggtgttat ctcagcggtt
     4201 gcaattttat tggcattaag catataacca actaacgctc cgctggaatt agaatccaca
     4261 ggaatcttat cagtttttag agcccatact ttaaactggt aatgatgggg tttatctcct
     4321 ttgggaggac atgctccacc aaatcctgca taaccaaaat catttcggcc ttgaacagca
     4381 ccagtcggaa gttttgtacc atcacgtctt cctgcatcag tgggcaaata tgttacagtt
     4441 gctggaatat tagcaacagt ccagtgccac catccactgc ctgtaggagc atctggatca
     4501 tacacagtta cggcaaagct tttggtgcct tcaggagcac cagaccaggt taatgagggc
     4561 gatgtattac caccttcaca cccaaatcca gaaaatacat gagacgttgt aagttgctct
     4621 cctgttttta tttcattact agtgacctga aatgctgcgg cctgcgcaga aaatgttatg
     4681 aatgccaata cacttgaaac gataagtttt ttcataaaaa cctcattgtt gtgacctatc
     4741 gttattttat ttgatgtgcc ttgatctcat tatgcataaa ggcgcaatga tcgcgcaaat
     4801 gcaatcataa ttataaaggt gccccgaaca ttagccataa tatgcacaaa tcagattcct
     4861 gttatctcaa ggagaattat tttttaatgc ggtgcacttc gtgacaacaa gttaatgact
     4921 aacacaccgg cacaaatcaa catcatgcct atgatagctg gcaggtccag tcgttgacca
     4981 aaaaatcccc aggaaagcaa actaatcaga acgataccga ctcctgacca gatagcataa
     5041 gcaatccctg taggtatata agccagcgtc tgagctaata accagaatga tgcacaatag
     5101 caaataattg tgccaacaga gggccataac cgtgtaaacc cctctgaaaa cttcattaag
     5161 gttgtaccaa tgacctctgc aagtattgcg ccaccaagat aaatataagg gttcatagca
     5221 tattctttcc tgttcatact ggagagaatt gtactacagt ttgaacacaa ctcaccgttt
     5281 tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc aagagataaa ggcaggaaca
     5341 ataatgatca gaaggaaccc caggctaacg aatgagttca ctcttcctgg gagcaaagcg
     5401 aagacaaaac atgttatcgc gtttacggat cgtggatggc agaaaacaac caggatcagg
     5461 tatccatttt aaaccagaaa ttgagtgagt ttgccccatc catgccccac gctgtgggat
     5521 cggatataat taaacaagca tagtcaacag gttacacaac ctacacctgc atggacatca
     5581 cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg
     5641 aggctttttg catatcggtc atcacatcgt ttagcgccac gccgggttca ccgagggtga
     5701 atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct tatgcgatcg agcgcggcgt
     5761 gcagctgccc ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca cgcgcactca
     5821 tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttcg
     5881 tctcccggat aatttctggt aacaaagcct accagtaagt caataagaca aaggcgctaa
     5941 atagcaacaa aaaaactggt ttattggcgg atagaaaaaa cgaaagcaca aataatggga
     6001 gcgtcaattt ttcgagtgtg ctgacccgag agtgagtctt gttccacttt gccaataacg
     6061 ccgtccataa tcagccacga agtgcgcgat gaatgcgact gcagcccaga caaaatctct
     6121 tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgtta ccggttccgc
     6181 ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt
     6241 attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa
     6301 tattccttac cgcttcagcg aagccagcgg cgctattgag gttccggcag ataaagttca
     6361 cgaactgcgc ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct
     6421 cgatcaggaa aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct
     6481 ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca
     6541 tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt
     6601 gacggtaaat ctgttacccg gccgcgcact cgatgaaggg caaattagcg ctattgtaca
     6661 tctggtttcc agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg
     6721 cgggcatctg ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa
     6781 atatgccagc gatgtcgaag gccgtattca gcagcgtatt gaagcgatcc tgtcacctat
     6841 tgttggtaac ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga
     6901 acaaacggaa gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg
     6961 ccagcttaat gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt
     7021 gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa
     7081 taaccgccag cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa
     7141 tgaaaccagt aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga
     7201 tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc
     7261 attgcctctc agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg
     7321 cttttctgaa aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga
     7381 cgaaagcggc ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc
     7441 tgccggtcgc tggttgctgg ttgctggtac tgctggtggc gtggttgctg tggcggaaag
     7501 cggtacgccc gcagctaaca cgtcgcgccg aggcggtgaa gactgtgcag caacaggcgc
     7561 aggcccgcga ggaagtggaa gatgcggtgg aagtccgtct gagcaaagac gaacaactcc
     7621 aacaacggcg cgctaaccaa cgactggggg cagaagtcat gagccagcgt atccgtgaaa
     7681 tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc
     7741 atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac
     7801 cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca
     7861 atggcgaacg tcacgcagat ctccaacaag cagctaaccg atgtgctggc ggagtttgag
     7921 caagaagctg aacagtttgc tgcactgaat atcaacgcca acgattatct gcgttcggta
     7981 ttggtcaaag ctctgggtga agaacgtgcc gccagcctgc tggaagatat tctcgaaacc
     8041 cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag cgccgccgat
     8101 ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct gaggcgcgcc
     8161 caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt
     8221 atcgccacct ttggcggcgt gcagccagcc gcgctggccg aactgaccga agtactgaat
     8281 ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc
     8341 gaaattatca acctgatgaa aacgcagcag gaagaagccg ttattaccgc cgtgcgtgaa
     8401 ttcgacggcg agctggcgca gaaaatcatc gacgagatgt tcctgttcga gaatctggtg
     8461 gatgtcgatg atcgcagcat tcagcgtctg ttgcaggaag tggattccga atcgctgctg
     8521 atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag
     8581 cgtgccgccg atattctgcg cgacgatctc gccaagcgtg gtccggtgcg tctgtcgcag
     8641 gtcgaaaacg aacagaaagc gattctgctg attgtgcgcc gtcttgccga aactggcgag
     8701 atggtgattg gcagcggcga ggatacctat gtctgataat ctgccgtgga aaacctggac
     8761 gccggacgat ctcgcgccac caccggcaga gtttgtgcca atggtcgagt cggaagaaac
     8821 cattattgaa gaggccgaac ccagccttga gcagcaactg gcgcaactgc aaatgcaggc
     8881 ccacgagcag ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc acgaacaggg
     8941 ctatcaggaa ggactggccc aggggctgga acaaggtctg gcagaggcga aggctcaaca
     9001 agcgccaatt catgcccgga tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc
     9061 gcttgatagt gtgatagctt cgcgcctgat gcagatggcg ctggaggcag cacgtcaggt
     9121 gatcggtcag acaccaacgg tggataactc ggcactgatc aaacagatcc aacagttgtt
     9181 gcagcaagaa ccgttattca gcgggaaacc acagctgcgc gtacacccgg atgatctgca
     9241 acgtgtggat gatatgctcg gcgcaacttt aagtttgcat ggctggcgct tgcggggcga
     9301 tcccaccctc caccctggcg gatgtaaagt ctccgccgat gaaggcgatc tcgacgccag
     9361 tgtcgccact cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaatgacc
     9421 acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg
     9481 cctgcggtac gtcgctacgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc
     9541 ggattacaat tgccgctcgg cgcaacctgt gtcattgagc gccagaacgg cagcgaaacg
     9601 cacgaagtag aaagcgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg
     9661 gaggaagtgg aaggtgtcct gcccggcgcg cgtgtatatg ccaaaaacat ttcggcagaa
     9721 gggctgcaaa gcggcaagca gttgccgctc ggtccggcgt tattaggtcg tgttctggac
     9781 ggtagcggta aaccgctcga tggcctgccc ttccccgata cgacggaaac cggtgcgctg
     9841 attaccccgc catttaaccc gttgcaacgt acaccgattg aacatgtgct ggacaccggc
     9901 gtgcgcccaa tcaatgccct gcttaccgtc ggacgcgggc agcgtatggg gctgtttgcc
     9961 gggtcgggcg ttggtaaaag tgtgctgctg gggatgatgg cacgctacac ccgcgccgat
    10021 gtcattgtcg tgggtttgat tggtgaacgt gggcgcgaag taaaagattt tattgagaat
    10081 atcctcggtg ccgaaggacg tgcacgctca gtggtgattg ccgctccggc ggatgtttct
    10141 ccgctcctgc gaatgcaagg tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat
    10201 cgtggtcagc atgtgttgct gattatggac tccctcaccc gctacgcgat ggcccagcgt
    10261 gaaattgcgc tggcgattgg cgaaccacct gccactaaag gttatccacc gtcggtgttt
    10321 gccaaattac cggcactggt cgagcgtgcc ggtaatggca ttagcggcgg cggctcgatt
    10381 accgcgtttt ataccgtgct caccgaaggc gatgaccagc aggaccccat tgctgactcc
    10441 gcgcgggcaa tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga agccgggcac
    10501 tatccggcta tcgatattga agcgtcgatc agtcgcgcaa tgacggcgtt gatcagtgag
    10561 caacattacg cgcgagtgcg caccttcaaa cagctgttgt cgagttttca gcgtaaccgc
    10621 gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga taaagccatc
    10681 gccctgtggc cgcagctgga gggctatttg caacaaggca tttttgaacg cgcggactgg
    10741 gaagcgtctc tccaggggct ggagcgtatt ttcccgacag tgtcataacc caggagataa
    10801 cggcagatgg cggaagaaca gctcaaaatg ttgatcgatt atcagaatga atatcgcaat
    10861 aacctcaata gcgatatgag tgccgggatg accagcaacc gctggatcaa ctatcagcag
    10921 tttatccaga cgctggaaaa agccattact cagcatcgcc agcaacttaa tcagtggacg
    10981 cagaaagttg acattgccct gaacagctgg cgagaaaaga aacaacgttt gcaggcctgg
    11041 cagacactgc aggaacggca atccacggcg gcactgcttg cagaaaaccg cctcgatcag
    11101 aaaaagatgg atgagttcgc ccagcgcgcc gccatgagga aacctgaatg attcgcttag
    11161 cgcccttgat taccgccgac gttgacacca ccacattgcc tggcggcaaa gccagcgatg
    11221 ctgcacaaga ttttctcacg ttgttgagcg aagcattagc aggcgagaca acaactgaca
    11281 aagcggcccc ccagttgctg gtagcaacag ataagcccac gacaaaaggc gagccgctgg
    11341 tcagcgatat tcttgccgat gcgcaacaag cggatttact gatccctgtg gatgaaacac
    11401 ttcctgtcat caacgacgaa caatccacat caacaccgtt aaccaccgct cagacgatga
    11461 cgttggctgc ggtggctgac aaaaatacga caaaagacga aaaagcggat gatcttaatg
    11521 aagacgtcac cgcaagtctg agcgcccttt ttgcgatgtt gccgggtttt gacaatacgc
    11581 ccaaagtgac tgatgcaccg tcaaccgtgt taccggcaga gaaaccaacg ctcttcacaa
    11641 aactgacttc tgcgcaactc actacagcac agcctgatga cgcccccggc acaccagctc
    11701 agccattaac accgctggta gcagaagccc agagtaaagc ggaagtcatc agcacgcctt
    11761 cgccggtgac cgctgacgcc agcccgctaa tcactccaca ccagacacag ccactgccca
    11821 ccgtcgccgc gcctgttttg agtgcaccgc tgggttctca cgaatggcaa caatcattga
    11881 gc
//
LOCUS       DAJTMX010000116        11787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTMX010000116 DAJTMX010000000
VERSION     DAJTMX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.116"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..104)
                     /locus_tag="OO145_003196"
     CDS             complement(<1..104)
                     /locus_tag="OO145_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5468219.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO145_003197"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO145_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5468220.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="OO145_003198"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="OO145_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5468221.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="OO145_003199"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="OO145_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5468222.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2423..2839
                     /gene="csgF"
                     /locus_tag="OO145_003200"
     CDS             2423..2839
                     /gene="csgF"
                     /locus_tag="OO145_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5468223.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2866..3572
                     /locus_tag="OO145_003201"
                     /pseudo
     CDS             2866..3572
                     /locus_tag="OO145_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3705..4196)
                     /locus_tag="OO145_003202"
     CDS             complement(3705..4196)
                     /locus_tag="OO145_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5468224.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OO145_003203"
     CDS             complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OO145_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5468225.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OO145_003204"
     CDS             complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OO145_003204"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5468226.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OO145_003205"
                     /pseudo
     CDS             complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OO145_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6841..6928
                     /locus_tag="OO145_003206"
     tRNA            6841..6928
                     /locus_tag="OO145_003206"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6875..6877,aa:Ser,seq:gga)
     gene            complement(7078..7359)
                     /locus_tag="OO145_003207"
                     /pseudo
     CDS             complement(7078..7359)
                     /locus_tag="OO145_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7641..8645)
                     /locus_tag="OO145_003208"
     CDS             complement(7641..8645)
                     /locus_tag="OO145_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5468227.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8717..9277)
                     /locus_tag="OO145_003209"
                     /pseudo
     CDS             complement(8717..9277)
                     /locus_tag="OO145_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9362..9559)
                     /locus_tag="OO145_003210"
     CDS             complement(9362..9559)
                     /locus_tag="OO145_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468228.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9571..10062)
                     /locus_tag="OO145_003211"
     CDS             complement(9571..10062)
                     /locus_tag="OO145_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468229.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10059..10433)
                     /locus_tag="OO145_003212"
     CDS             complement(10059..10433)
                     /locus_tag="OO145_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5468230.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10480..10854)
                     /locus_tag="OO145_003213"
     CDS             complement(10480..10854)
                     /locus_tag="OO145_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5468231.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11017..11238)
                     /locus_tag="OO145_003214"
     CDS             complement(11017..11238)
                     /locus_tag="OO145_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5468232.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11301..11777)
                     /locus_tag="OO145_003215"
     CDS             complement(11301..11777)
                     /locus_tag="OO145_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5468233.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
BASE COUNT         3068 a         2856 c         2760 g         3103 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagactcc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac ggacgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata actttactca
     2641 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     2701 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     2761 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     2821 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     2881 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     2941 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     3001 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     3061 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccagggctta caaaacctgc
     3121 ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     3181 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     3241 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     3301 cgcaatacca gctcgatcag attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg
     3361 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     3421 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     3481 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     3541 atggtatcga ccgtggtctg tgggatttgt aaaataaagc agaacggcag aatgacattc
     3601 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     3661 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     3721 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     3781 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     3841 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     3901 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     3961 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     4021 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaagcaaag
     4081 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     4141 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     4201 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     4261 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     4321 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     4381 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     4441 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     4501 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt agcatccctc
     4561 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     4621 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     4681 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     4741 cagccagttt cccctcacga atcagagtaa acagcggcac cagtaaagga tcttgcggtt
     4801 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     4861 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     4921 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctagcg gaaaatctac
     4981 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     5041 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcagctaccg cacgacagtt
     5101 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     5161 atgtttcgcg gctgcttcag caacggcttt aacatctatt tcatatttcg gatttccggg
     5221 atggcttatt atatgcacat tgccgctggc gatagtggag atcatcgctt gtgtattggt
     5281 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     5341 gtcaaacatt ttaccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcaat
     5401 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     5461 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     5521 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     5581 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     5641 aaggatagca ggaatcctga tgctttatta gtagccacgt gcgcggtcga cttgcccgca
     5701 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     5761 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     5821 actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc
     5881 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     5941 gagatacgcg ccatcaggta atttttcgag taattgttga ttaataatgc cgacggtttc
     6001 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     6061 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     6121 cagcggaaag cgctaggttt gcagactctg agcaacttta cagcccagta cgcctgcgcc
     6181 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     6241 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     6301 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     6361 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     6421 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     6481 aacataatca gcagagtcat tatctccgct tttccatgcc ctgactcttg cctgaggaat
     6541 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     6601 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     6661 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     6721 taaaaccaca cattgattga atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg
     6781 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga acgacaattt
     6841 ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc
     6901 gggggttcga atccccccct caccgccaga ttataagtgc atgttattgt ttaacatgcg
     6961 cttttttatt tttccttcca gacagtatgc catcgggtat gccatctgaa accggtttat
     7021 atgccccgcg atcatatctt atcggacaat ttccgatctt tgagcatact ggcaaagcta
     7081 tactgccagc atatcaggat agtgttttgc gataatatca ttcagtctgt caaccagttc
     7141 tggttttctg aacgtgatat gcgcggagcc cttcttaaag tatctgatgc tgaacatctc
     7201 atcttcatag ctctctttac cctgaacaga ctgaatgtga tcatccagac gaagggtgat
     7261 gtttccccgg ttgtcgggga ttggtttacc gctgaacagg tgcaacattc tttccagatc
     7321 ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat ctatggtcac ccccgttttt
     7381 gcaacactga tttttgatta atggttggct tgcttaaatc tatccggcgt ctctgtgggc
     7441 aaggatgccc gcgccccgat gagttccgca cctgataatc ctaaaaatcc cctcggcttc
     7501 agtgagccat ttcatctgtt aggtttttaa tcaggtcgat ttgccgttca tgtcatcaat
     7561 aatcagtctt cgcaaaacca gaaaggtaac ttgtatctta ttgtttaaaa taaactaatt
     7621 gagttatatc agcatctata tcaggcctga tattcttttt tctgagtagt gattgcccag
     7681 gcaattcgag ccagtttatt tgccagagcg caggtaacca cattcgaatg cttcttgttg
     7741 agttgctccc gaacccactc agcaagtttt ccatgcttgc gttcaagtcg cattatgaat
     7801 gagcgggcac actgaaccag taaccggcgt aaatttttat cacctcgctt actgataccc
     7861 aacagagtcg attttcctcc agtgctatac tgccggggaa caagtcctgt ggaagctgca
     7921 aaatccctgc tgcatgagaa ctgtttcccg tcaccaagtt gcgacgatag taagctggca
     7981 gtaatcggac caacaccagg aattgtcata atgcgctgag cggtatcatc agcgttgatt
     8041 gactgactta attctgattc cagctctgct atttgctcaa caaggtaaaa gtagtgggta
     8101 tgcaacctca ccaaaaggcg gctcagatat tcggggattt cgtgttcagc caacacgaga
     8161 gacaaccgtt ttattacagc atcacctatc ggcaggctga ttccaaattc cagcaagaaa
     8221 ccgtggatct ggttggttgt tttcacctta tctctgatga gcgattctct gacccggtgt
     8281 aacgccctca tggcctgctg ggtctcagtt cttggctgga caaaacgcat tgatggtcgt
     8341 gaagccgctt cgcatatggc ttctgcatca acaaaatcat tcttattact tttgacaaac
     8401 gggcgaacga actgtggaga aatcagctta gtttcatgcc caaaatcaga gatacagcga
     8461 gccataaagt gtgcacccgc acaggcttcc attacaacaa ctgagggcgg acatgtcgcc
     8521 agaaactgca tcagtttagt acgggtaaat tttttacgca gaagagcctt accagacttg
     8581 tcttgacaat gaatgtggaa ggagtgcttt ccgagatcga taccaataag tgtgatgttc
     8641 tgcatgatgg tctctccgtt aaagggattt accttaacag cgtactgctg ttaaggtggg
     8701 ggtgaccata tcattatccc caccggtccc atctcaccag attgttgacg ataattttac
     8761 tgccaaattt acaggggcaa ttggttttgt aattccagtt cagccctctg aagacgttga
     8821 tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagaa
     8881 tgttcgcttc gctgatttcc ggaaaattat cgtactccag tgaccggtac catgtatcac
     8941 gggcctgcgt atccattaat gacagcatcc cggaacgttg catcaggtca cgccagattt
     9001 cgcgatcaag attccgggta atggctttca ttgctgtttc gggtttctcc atcagccagc
     9061 agccgcagcg aaaatcctgt ttcatggccc agtccggggc agttttacct ccgatgcttt
     9121 ccgtcagcac cgagatttca gccagttgtc ggataagtgt gtcgatttgc aacagtgtac
     9181 tctttcgtac agccagaatg tggcgaatgc tggttgagca gataacgtcc gtatgcccgt
     9241 tgaccacttc cggacgggag attgtgatat ctgacataat cgatattcct ttttgcagat
     9301 aaatgaaaac gccagccctg tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc
     9361 ttcaccggtt ctggtgcaga agctgcagat gacgcagggt acggacatcc tcccgggcct
     9421 gttcgataca gggcaacagt gctgctgaat ccgttttatc ctcatcgttg tcaaagataa
     9481 ttcccgatcc gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agcgctgttt
     9541 ccgtggttaa tgatttcatg ttgttcctgg ttaccagtcg aaagtgggta aacccggcag
     9601 gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgccgga tgagtgtgac
     9661 gaccagacga cggaacgttt ttgacagccc caggcgttta agccgcagaa ccgggtatga
     9721 ccacgcatcg gtacgtaaca gatatccgct acctgtaaaa tgaatccatt ctgattcacc
     9781 gaagtcactg gtctggtgtg acagcgagta caaccaggcg ttgtccgttt ccgtgatatg
     9841 tgcggtactg cagcgtatgc cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt
     9901 ctggtcagat tcatcaacaa cacgaaggga gtagccgtta tcgcagacca tgttaatcag
     9961 ttcagcgagc tcaataccat cgacgtcaac gacaatgcgc cctatgttca gtgcctgtac
    10021 gttaacgctg tcggcttcca gtgtcagggc cagtttcatc atttcgcctc cggatactta
    10081 cccagggtaa tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg
    10141 cgagcccgga ggatatcaat actgtttatt aactgagagc ggggacaggt gctgaatccc
    10201 ggctggtcgg tacgcaccag cgcgtatttt tccacgagaa aattaactgc atcacacagt
    10261 gaaatgcctg cctcaatatg ctgctcaatc acacgttcat cgacaaacgg tgtgtcattc
    10321 agtgtgaggc cataatgctg gtccagcagt cggatgagca gtgtctgcca gatggtgacg
    10381 ggagacgggc agcgagatac ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt
    10441 tctctgtaac tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg ttgcgggtgc
    10501 tggtgtcgga taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgca
    10561 ggtcagccct tttgcataca gcgtgacggt atgctgatat cggggattca gttcaccgct
    10621 ggtgagcatg agttccagtt gtttcatcag cagcggaaag gcctggtcca gatggtatgc
    10681 atctgcgtcg ctgaagcggc ctctgatacc ggcgcggtcg gcaaggtaat acaggcggtt
    10741 accctcttgc accagacgag cgccaaaaca gggcctcact gtgcagggaa gcccccacca
    10801 ggggcggtcg tgattgtcat cgggatgagt tatcccggag agtttgtctg acacgataaa
    10861 atcctctctg aaaagtggtt aagaatggtt taacgatgac aggtcagccg gcgcgctgac
    10921 cggtttcggt gaagatgtat gcattgatga gaatggcctc atccacagcg gcgtctgagg
    10981 tcatctggtc gtactcgttt cccgggtgac ggaacatcag ttcagggtga cactcatcag
    11041 ccggacatac gggccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg
    11101 aatatcctgc acttcacgac cagtatacgc ttcagcactg ccgtgccagc gatacttacc
    11161 ggtacagaac ggaaaaagac gggatgcagg atgctgttgg tgaatacgca tggcttcacc
    11221 acgggtgatg attctcatga tgtgatacct ctgaagacag aagataaaag tgaaaacagg
    11281 tgtgatgtgg ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggttg
    11341 ccaccgacta tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga
    11401 cgttcggtga taagccggtc tgccttactg ggtgtgactt caccggacgg gtgattgtgc
    11461 gccagcacca cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatga
    11521 acttccgtac ggttgatggt gccggtgaag agggtttcac cggcaatcag ctgattctgg
    11581 ttgttcagat acagcacccg gaactcttcc cgctccagtc ccgccatgtt cagaatcagc
    11641 cattcccgtg ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc
    11701 agggtttgca gggcccgcag aatgagactg cgctcgccgg gcgtcatgtt tccgggcaga
    11761 aaggaaagct gctgcattgt ccctgcc
//
LOCUS       DAJTMX010000117        11657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTMX010000117 DAJTMX010000000
VERSION     DAJTMX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.117"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            597..794
                     /locus_tag="OO145_003216"
     CDS             597..794
                     /locus_tag="OO145_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468234.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO145_003217"
     CDS             complement(744..1937)
                     /locus_tag="OO145_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5468235.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO145_003218"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO145_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5468236.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO145_003219"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO145_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5468237.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO145_003220"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO145_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5468238.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO145_003221"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO145_003221"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5468239.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO145_003222"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO145_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5468240.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO145_003223"
     CDS             complement(10573..10716)
                     /locus_tag="OO145_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468241.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11657
                     /locus_tag="OO145_003224"
     CDS             10955..>11657
                     /locus_tag="OO145_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5468242.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT         2956 a         2898 c         2992 g         2811 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggact
//
LOCUS       DAJTMX010000118        11634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTMX010000118 DAJTMX010000000
VERSION     DAJTMX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.118"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..346
                     /locus_tag="OO145_003225"
     CDS             <1..346
                     /locus_tag="OO145_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5468243.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO145_003226"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO145_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5468244.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO145_003227"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO145_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5468245.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO145_003228"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO145_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5468246.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO145_003229"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO145_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5468247.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO145_003230"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO145_003230"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5468248.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO145_003231"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO145_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5468249.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO145_003232"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO145_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5468250.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO145_003233"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO145_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5468251.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO145_003234"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO145_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5468252.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO145_003235"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO145_003235"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5468253.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO145_003236"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO145_003236"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5468254.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO145_003237"
     CDS             complement(8961..9236)
                     /locus_tag="OO145_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5468255.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO145_003238"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO145_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5468256.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO145_003239"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO145_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5468257.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO145_003240"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO145_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5468258.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO145_003241"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO145_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5468259.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..>11634)
                     /locus_tag="OO145_003242"
     CDS             complement(11073..>11634)
                     /locus_tag="OO145_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5468260.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         2752 a         2861 c         3076 g         2945 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgtt
//
LOCUS       DAJTMX010000119        11586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTMX010000119 DAJTMX010000000
VERSION     DAJTMX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.119"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(282..357)
                     /locus_tag="OO145_003243"
     tRNA            complement(282..357)
                     /locus_tag="OO145_003243"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO145_003244"
     CDS             916..3168
                     /locus_tag="OO145_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468261.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKIWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4436
                     /gene="csiD"
                     /locus_tag="OO145_003245"
     CDS             3504..4436
                     /gene="csiD"
                     /locus_tag="OO145_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5468262.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4456..5724
                     /gene="lhgO"
                     /locus_tag="OO145_003246"
     CDS             4456..5724
                     /gene="lhgO"
                     /locus_tag="OO145_003246"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5468263.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5747..7195
                     /gene="gabD"
                     /locus_tag="OO145_003247"
                     /pseudo
     CDS             5747..7195
                     /gene="gabD"
                     /locus_tag="OO145_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7203..8489
                     /gene="gabT"
                     /locus_tag="OO145_003248"
                     /pseudo
     CDS             7203..8489
                     /gene="gabT"
                     /locus_tag="OO145_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8727..10127
                     /gene="gabP"
                     /locus_tag="OO145_003249"
     CDS             8727..10127
                     /gene="gabP"
                     /locus_tag="OO145_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5468264.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10148..10810
                     /gene="csiR"
                     /locus_tag="OO145_003250"
     CDS             10148..10810
                     /gene="csiR"
                     /locus_tag="OO145_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5468265.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO145_003251"
     CDS             complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO145_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5468266.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11344..11502)
                     /locus_tag="OO145_003252"
     CDS             complement(11344..11502)
                     /locus_tag="OO145_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5468267.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2980 a         2846 c         2889 g         2871 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaatatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 gggggaagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgctgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg
     4081 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
     4141 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
     4201 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
     4261 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     4321 tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     4381 tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc
     4441 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     4501 accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag
     4561 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     4621 tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc
     4681 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct
     4741 cagctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     4801 cgcgagtgga taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt
     4861 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca
     4921 aaaatcttcc aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt
     4981 gagcataaaa acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg
     5041 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     5101 ggctttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     5161 attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat
     5221 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     5281 cgcgaaggct atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg
     5341 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     5401 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     5461 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag
     5521 ctgattgacg attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc
     5581 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     5641 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     5701 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     5761 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     5821 tgaagtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     5881 gggcgcagat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg
     5941 tgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca
     6001 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc
     6061 gaaaggcgaa atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg
     6121 tatttatggc gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca
     6181 gccgattggc gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg
     6241 taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac
     6301 gccgttctct gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt
     6361 gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct
     6421 ggtacgcaaa ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg
     6481 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     6541 tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc
     6601 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     6661 tgccgaaaaa ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg
     6721 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     6781 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg
     6841 caacttcttc cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga
     6901 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     6961 acaagccaat gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg
     7021 cgtcttccgc gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat
     7081 ttccaatgaa gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     7141 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     7201 gaatgcgaag gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     7261 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     7321 tttaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     7381 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     7441 tccaggtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     7501 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     7561 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt
     7621 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     7681 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     7741 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     7801 aagatatcgc cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     7861 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     7921 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     7981 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg
     8041 gcgtcaccgg gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct
     8101 atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg
     8161 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga
     8221 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg
     8281 agctgtttga agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg
     8341 cacgcgcccg cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     8401 gcatccttgt accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg
     8461 gtcagtgttt tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc
     8521 gtcttgtccg gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc
     8581 tgtacgtgaa caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa
     8641 aagaatcaat aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa
     8701 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg
     8761 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     8821 tgtttgtcgg ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc
     8881 tgttcgccgg attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca
     8941 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct
     9001 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca
     9061 tcgccgccat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     9121 tcattaccct cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     9181 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     9241 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     9301 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     9361 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa
     9421 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     9481 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     9541 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     9601 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     9661 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     9721 aaaccccgta cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga
     9781 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     9841 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     9901 aaggaagcga aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg
     9961 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
    10021 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
    10081 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
    10141 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
    10201 cggtaatttt caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact
    10261 tggcgttggg ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt
    10321 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
    10381 cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga
    10441 tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
    10501 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
    10561 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
    10621 ggcgcgttat cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga
    10681 taaacacgat cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc
    10741 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
    10801 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
    10861 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
    10921 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
    10981 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
    11041 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
    11101 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
    11161 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga
    11221 gtttttctcc ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg
    11281 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
    11341 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
    11401 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat
    11461 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
    11521 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
    11581 gccagt
//
LOCUS       DAJTMX010000120        11514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTMX010000120 DAJTMX010000000
VERSION     DAJTMX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.120"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            38..778
                     /locus_tag="OO145_003253"
     CDS             38..778
                     /locus_tag="OO145_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5468268.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="OO145_003254"
     CDS             837..1223
                     /locus_tag="OO145_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5468269.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="OO145_003255"
     CDS             1232..1561
                     /locus_tag="OO145_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5468270.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="OO145_003256"
     CDS             1533..4574
                     /locus_tag="OO145_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5468271.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="OO145_003257"
     CDS             4574..4903
                     /locus_tag="OO145_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5468272.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="OO145_003258"
     CDS             4903..5601
                     /locus_tag="OO145_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5468273.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="OO145_003259"
     CDS             5606..6349
                     /locus_tag="OO145_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5468274.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6286..6888
                     /locus_tag="OO145_003260"
     CDS             6286..6888
                     /locus_tag="OO145_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5468275.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="OO145_003261"
     CDS             6949..10428
                     /locus_tag="OO145_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5468276.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="OO145_003262"
     CDS             10496..11095
                     /locus_tag="OO145_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5468277.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11117..>11514
                     /locus_tag="OO145_003263"
     CDS             11117..>11514
                     /locus_tag="OO145_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5468278.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         2693 a         2717 c         3704 g         2400 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcggt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggc
//
LOCUS       DAJTMX010000121        11353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTMX010000121 DAJTMX010000000
VERSION     DAJTMX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.121"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..527)
                     /locus_tag="OO145_003264"
     CDS             complement(<1..527)
                     /locus_tag="OO145_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5468279.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPR"
     gene            complement(539..2647)
                     /gene="rlmKL"
                     /locus_tag="OO145_003265"
     CDS             complement(539..2647)
                     /gene="rlmKL"
                     /locus_tag="OO145_003265"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5468280.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            2891..4000
                     /gene="ycbX"
                     /locus_tag="OO145_003266"
     CDS             2891..4000
                     /gene="ycbX"
                     /locus_tag="OO145_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5468281.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(3997..4539)
                     /gene="zapC"
                     /locus_tag="OO145_003267"
     CDS             complement(3997..4539)
                     /gene="zapC"
                     /locus_tag="OO145_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5468282.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(4713..5723)
                     /gene="pyrD"
                     /locus_tag="OO145_003268"
     CDS             complement(4713..5723)
                     /gene="pyrD"
                     /locus_tag="OO145_003268"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5468283.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(5834..6571)
                     /locus_tag="OO145_003269"
     CDS             complement(5834..6571)
                     /locus_tag="OO145_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5468284.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            complement(6537..7052)
                     /locus_tag="OO145_003270"
     CDS             complement(6537..7052)
                     /locus_tag="OO145_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5468285.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(7060..7600)
                     /locus_tag="OO145_003271"
                     /pseudo
     CDS             complement(7060..7600)
                     /locus_tag="OO145_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(7698..8681)
                     /locus_tag="OO145_003272"
     CDS             complement(7698..8681)
                     /locus_tag="OO145_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5468286.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(8672..11272)
                     /locus_tag="OO145_003273"
     CDS             complement(8672..11272)
                     /locus_tag="OO145_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5468287.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
BASE COUNT         2837 a         2986 c         2632 g         2898 t
ORIGIN      
        1 cgcggattgc tcaccagcgc gcgtcctaat gccgctttac gcaaccagcc gccggaaagc
       61 gacgacagcg caacgttagg atctaacccc agttgcgcca gcacttcgtt gatgcggttt
      121 tccagctgcc acaggttgtg gtgatccagc tgttcctgaa ccttcgccag ttcgttgaga
      181 tttttctcgc tcgggtcgtt catcaccagg cgcgaaatat cgtgatagcg tttcagatat
      241 tccgcttgtt cttcaatgcc ttcggcaacg aaatcataaa cgctaccctc aacgttacgc
      301 ggcggatcct gttgcagacg agctacaatc aaatcttgct cataaataat gcgaccgtca
      361 tccagccctt gttcacggtt gaggattttc attaacgtcg attttcctgc gccgttgcgg
      421 cccaccagac aaacacgttc gttatcttcg atatgcagtt ctgcgttatc gagaagcggc
      481 gcgtcgctga acgacagcca tgcgccatgc atactgatta atgacattac tattcctttc
      541 aggctgcggt aatcagccag cagttgtgga tctggcggtt acgggcgaaa tcctgggaga
      601 gcgttttttg cgtaatttct tgtgctttca gtcccagttt tgccaggccg tcgagatcca
      661 tacggaagcc acgtttgttg ttcgagaaca tgatcgtccc acctgcacgc agcagacgtt
      721 tcaaatcttt catcagcgcc agatgatcgc gctgaacatc aaacgcatct tccattcgtt
      781 ttgagttaga gaaggttggc ggatcgataa agatcagatc gaactgttca tttgcctcac
      841 gcagccacgc caggcaatcg gcctgaatca ggcgatgcgc acgcctagtc aggccattca
      901 ggcgcaggtt gcgttctgcc cattccagat aagtacgcga catatccacg gtggtggtgc
      961 tgcgtgcacc gcctaatccc gcatgcacgg tggcgctgcc ggtataagaa aacaggttga
     1021 ggaaatcttt gcctttgctc atctgaccga gcatacggcg ggcgatacgg tgatcgagga
     1081 acagaccagt atcgagataa tccgtcaggt tcacccacaa gtgagcgtta tattcggtaa
     1141 cttcaagaaa ctcgcccttc tcgcccagtt tctggtactg gtttttgccc ttatggcgtt
     1201 cacgggtttt cagcaccagt ttgtttggcg caatccccag taccgaaatg gttgcagcga
     1261 taatatcgaa cagacgctga cgcgctttgt gcgcatcaat agtttttggc ggcgcatact
     1321 cctgcaccac cacccagtcg gcataacggt caacggcaac gttatattcc ggcagatcgg
     1381 cgtcatacag gcggtaacat tcaatccctt cctggcgtgc ccacttctcg aattttttga
     1441 ggttcttacg caggcggttg gcgtaatctt ccgctaccat tgccggttta ctgtctggtg
     1501 tgctttcggc aacgtggtaa tttttctgta cgcagtccag cgggccgttt ttcgccttgt
     1561 actgtttgtc tgcacgcagc tgcaggcagc ttagcagatc cggcgaggca ctaaacaaag
     1621 agagattcca gccaccgaac tggtttttca tgattcggcc cagcagacta tgcagcgcaa
     1681 tcagcgccgg ttcgctgtcc agacgttcac cgtatggcgg gttgctcaac actgtaccgt
     1741 acggcccttt cggcagcgga ttggtcagtt gcgcgacatc tttcacctca aaggtgatca
     1801 gttcaccaat ccccgcaaga cgggcgttag tgcgcgcacg ttgaatcacc cgtgcgtcgc
     1861 tgtccgaacc gtaaaaatga gagctatact cagctaggcc tttacgggcg cgagtttgcg
     1921 cttccgcttt cacttcctgc cagatagctt catcatgctg cgcccagccg ctaaagcccc
     1981 aacgcccacg gtgcaagcct ggtgcgcgat cggtcgccag catcgccgct tcaatcagca
     2041 acgtaccgga accacacatc ggatcgagca gcggtgttcc tgactgccag ccggatcgca
     2101 tcacaatagc ggctgccagg gtttctttga tcggcgcaat accagcacga tcgcgatagc
     2161 cacgcagatg taaaccatca ccactcagat caagagcgat actggcggtt tctttatgca
     2221 gccagacgtt aacgcggata tccggcgcat cgcgatcaac atttggacgc ggcagatttt
     2281 tccgtgtgaa agcatcgacg atcgcgtctt tcactttcat cgcgccgtac tgactgttgc
     2341 ggatggtgtc attcaaacca ctgaagtgga cagcgaaggt cgcgccagga ttaaacatct
     2401 ctgtccagtt gatcgcctga acaccgagat agaggtctaa atcgctgtaa accttacact
     2461 cgcccagcgg caacataata cgcgaggcca ggcggctcca catcaggctc tggtaaacaa
     2521 gccgtgtgtc gcccttgaaa tggaccccac cctgaaccac ctggcattca acggccccca
     2581 gattttccag ttcagttttt aacagctctt ccagcccacg ggccgtactg gcaaacagag
     2641 aattcatatc gtcactttta ctctaagagc ctaccgcagt aggcgtaatt gttgcagtca
     2701 gtctggacat ggagagcgcg gagaaaccgg agcgtacaca agtacgtgag gatttcgagc
     2761 acaacccagg gccaaactga caaataaaat agcctaacgg gacaggcgct aagaaaattg
     2821 ttgcgcatta tagctaataa gcgcgtcatg tcataaagtt gagggcttat tttcatttga
     2881 ggaccgcacc gtggcgacat taacccggct ttttattcat cctgttaaat cgatgcgcgg
     2941 cattggtctt acacatgctc tggctgatgt cagtggtctg gccttcgatc gcatctttat
     3001 gatcacggaa cctgacggta cgtttattac cgctcgccag tttccccaga tggtacggtt
     3061 tactccttca cctgtgcatg atggcttgca tctcaccgca ccagatggca gtagcgcata
     3121 tgttcgtttt gctgatttcg ccacacaaga cgcaccaacc gaagtttggg gcacacattt
     3181 taccgcgcga attgcgccag acgcgatcaa caaatggcta agtggatttt tctcccgcga
     3241 agtgcaatta cgctgggtgg ggccacaaat gacccggcgc gtgaaacgcc acaacactgt
     3301 acctctgtca tttgctgatg gctatcctta ccttcttgct aacgaagcct cgttacgtga
     3361 tctccaacaa cgttgtccgg ccagtgtaaa aatggagcaa ttccgcccca atctggtggt
     3421 ttccggcgcg tcagcctggg aagaagatag ctggaaagtg attcgcattg gtgatgtggt
     3481 gtttgatgtg gttaaacctt gtagccgctg tattttcacc accgtcagcc cagaaaaagg
     3541 gcaaaaacat ccggcaggcg aaccattaaa aacattgcaa tctttccgca ctgcccagga
     3601 taacggcgat gtcgattttg gtcagaattt aattgcccgt aatagcggcg tgattcgcgt
     3661 tggcgatgag gtggaaattc tggcaacggc tccggcaaaa atttacggcg cagctgccgc
     3721 tgatgatacc gccaacatca cgcaacaacc ggacgcaaat gtagatattg actggcaggg
     3781 acaggcattt cgtggaaata accaacaagt gttgctggag caattagaaa atcagggaat
     3841 tcgtatccct tattcttgcc gcgcgggcat ttgtggaagt tgccgtgttc agcttttaga
     3901 aggcgaagtc acgccgctga aaaaatcagc aatgggcgat gatggcacca ttctttgctg
     3961 tagctgtgta ccgaagactg cccttaagct gacgcgttag actgcctgtt cgaggctgaa
     4021 gctgtcaaca ttcacctgcg gtttcagcct gtcgttcatg attttaatgg cgtcgcccaa
     4081 ctgcatcgcg cgacccgcaa taacaacgca gggttgtgcc agcaggcata gcgcggcatt
     4141 ttcgccagat tcaacgacca gcaaattgac ctgctcgtga gtatcgctca accaaacgca
     4201 ggccgcatcg cccggcatgg gcacccacat ttcaccatgg gagacaaaat gccagctttt
     4261 cggcatttgc ggtttgagat aacgaattgc taccagtgca ttcagcacca gttcggcttt
     4321 ttggtcttta gataaattaa aatcgcggca cttttcttca aaagagaaat agagcgcggc
     4381 atcatcaaca caaaagcctg cgggagaaaa ggcatctgga gtcaacattt tgcgcgcaaa
     4441 acgtgagcga aatagcatac cattggctaa atcgagcatc attcgatcgt gctcttcatc
     4501 gtaataccaa cgccagttat cgtctggttt aattcgcatc tgcctctccc ctaccctata
     4561 aaaccgcagc aacacaatcc attgtcgctt cgcttaataa cctcaacaat aaaagtacaa
     4621 aatgtctaaa taagcaacaa cgatgaatat aaaacaacca ggtctgaaaa taaagccctg
     4681 attatcttat aaagaggaat tcgaagaaat aattagatat gggtaacgat ttctttaatc
     4741 agcggcggac ctttaaaaat aaaaccagaa taaatttgca ccagtgaggc acccgcagca
     4801 atcttttcac gcgcagcgat aaccgagtcg atgccgccaa caccgatgat cggtaagcga
     4861 ccgtttaatt ctagtgacaa gcggcgaata atttctgtgc tttttaactg aagcggacga
     4921 ccacttaagc caccggtttg atcgcaattt ttcattccct gaaccagaga acgatcgaga
     4981 gtggtattgg ttgcaataac gccatcaata ttatggcgaa ctaaactatc ggcaacctgg
     5041 atcaattcgt cttcagaaag atccggcgcg atcttcactg cgatcggcac atatttatgg
     5101 tgcatcgctt gcaaatcatt ttgcttattt ttaatcgcgg ttaagagatc atccagcgct
     5161 tcaccatatt gcagcgtgcg taatcctggg gtattcggcg atgaaatatt gatggcgata
     5221 tatcccgcat aggcatagat tttttccata caaatcagat agtcatcttt gccctgctcc
     5281 actggcgtat ctttattttt gccgatatta atacccaaga cgccgtcata atgggccttt
     5341 tttacgttct ctacgaggtt atcaacgcca agattattaa agcccatacg gttgatcaaa
     5401 ccttcggcat ctaccagacg aaagagacgc ggcttgtcat taccaggctg tggacgtggc
     5461 gtgacggtac cgatctcgat cgatccaaat cccatcgcgc ctaacgcatc aatgcactcc
     5521 ccgtctttat caagaccggc tgccagacca agcggatttt taaacgtcag gcccatgcag
     5581 ttaacaggtt tcgcaggcac tttctgccgc accagtgctt caaacggtgt tccggtaatg
     5641 cggcgtaatt gctgaaaagt aaactcatga gcgcgctctg gatcgagctg gaaaagggct
     5701 ttacgaacga aggggtagta catgaactct cctggattcc cggtgtgcaa accgggggcg
     5761 tattatgtgc gagttcggac caaaagggaa ttgacctgcg gcaaaaaaaa cgcaaacgtt
     5821 ttcagaacct gtttcagggc agattttgct ccttctttgc actcaacccg ccgtaatcat
     5881 ttatcgtccg ccaggcgaca tgacacggtg ctattacctg acaccagttt gtctgacgct
     5941 ggcttttagg cggcacgact cccgcattac ttaacgcttt accatttacg ctcacctgaa
     6001 tcaggttaat gtagtaaggc gttgggttag tgagttgtac accctgcgaa ttccgtgtcc
     6061 agcgtaattg ttgataagct tccagcgggt cgcccggcaa accttcgggt cgccagaaca
     6121 gtttaaatac cgagcgcatc gccactttta cgctctgatt ttcaactttg ccggatggca
     6181 cgctggcaat gcttaactca aatagcgttt cgcgatccac aggcaagatg tcgctttccg
     6241 ttctcagcaa gcgcaacgtg cctgtagtac cgggcttcag gagaataagt ggcggagcgg
     6301 ccagtaatag tgctggcact gtcgacgctt cccccccggc ccaacgcgtt gggcgattga
     6361 ttttgctgtt aataagccaa gattcctgac tggtattagt cagtaaaata gatatcgatt
     6421 ctctgtctgc cggaaagata aatcgcgttc cgccgaccac cactcctgcc tgcaattcac
     6481 ccgccaccag cagcgataaa acggcgaaaa tcaagagcgc taatctgttc cacgtattag
     6541 tcataatgaa gtacaaatgt cgcgctggca ttcactttac ccgactgcac atcgctattg
     6601 acaggtcgca attgcgcata aaaagttaat ggcaccttac cgctatgtag gctgtagtct
     6661 ttactcgcct ggttaagcgg aatcaatatc ttcgcatcat ccagaatcgc aattgccagc
     6721 ccactggctg catccgtacc actttcaatg gacagcattt cgctctgcac tccgtcgggg
     6781 gttcctgaaa aggtgacatc aaccgtgctg acatctgttc cgcaatcctg caaattaatg
     6841 atgaaagatt tctccgggcc ataattccca tgaaaaaaac gtgtcagcga gacatcgcct
     6901 aactgcacat tgatttgctt gctatcctgt gccagcacgc atgtattacg gcgaaacgat
     6961 ccgctaatgt tgaccgtgcc atcatgggca aatagctcac agccccccgt taatcccagt
     7021 aagaacagaa tccagattat ccgtttcaac atcactcctt cactggtaaa ccagatcaaa
     7081 atagagaacc gccgtcgcat taccgcccgt tgctcccgcc tttgtggact tataacgaag
     7141 ttgatatttg agtgtgttat ccccggcttt taagggcgta agaggctgga cctgattgag
     7201 cgggatttct tgctgacttt gcgcatcaag aatgcacggc aatccccgtt gccataccac
     7261 cgcttccgcc tgtgtcagtt agtttgagca gtgtcggcgc aagggtgtca gcctcgccgc
     7321 taaaaaggac gtccgcgcca gtaataccag cggcacaacc ggtgatatca atattcaaat
     7381 cagcagcagt gcttacggta ttcgcggcct gaaaatcact gatattaaaa tcaccaatat
     7441 gaatattttg taatgcactt ttggtggcga catcacagcc ctgatcgacc acattcccat
     7501 aaatatgcat actgacttgt tcaggcccag ccatcccgac atggatgtta agaaggctaa
     7561 agaacaaaat aacgcactga tatatcgttt ttttcttcat acgttcacgc gttaatcaaa
     7621 atttattctc accgttgccg tggcttcaaa tcgcccggtt tctggtggta cgcttgtcgt
     7681 gctgactgga tagacattca ttcgcggata ctgtctgcat catcaggaat agagagcggt
     7741 agtgtcccac cagcagggga aataatgttg tgctggctat cggtcaccac cacgcccacg
     7801 ccaggacgtg acgtcttaat cgcctgggga tagctggggt ctgaggttgc cgtcagactc
     7861 agattgacgt taactaaccc tgcgtttccc gtgttacacc tgaccggaat tgcaagttct
     7921 gccgttctgg ccccaagagg cttatgccct acaacacgaa agttagccgc aaatacgtcg
     7981 cccagatcaa cctcgattgt ttctccggca ttgacggaac atgagccaag tgaattgatc
     8041 gtaccagaca ggtaatacac taaaaccggt gtaccagtag tcgtgcagga atcacctgcg
     8101 ggtatgttgt agcattcaaa taatcgcgcc agcgccatag gaggaatgac gaccgaacca
     8161 acgaacggct gcaatatttt cagggaaaag ctaccgctat taccgctagc cccctggcca
     8221 cctaaacgtt gaccgccggg atcgcaaata tgccatcgtg tatctgcccc tgtgcccacg
     8281 ttttcgaaag gaaccgcagt aggagaactg cctttaacct gcaataaaac tttggcggca
     8341 agatagtcat taattttgta ccagttgtcg cctaaataga ctaacggtga atccgcggcg
     8401 aatgcccatc ggccacttct gacattgtct ttatcgcaat cacaggaaac ataataatca
     8461 ttcgattcgt tccactgata aaatttgtct ttaactactc cagccgtatt ttcttcttca
     8521 tccagagttt taacgccaaa attgacgcca atatacggat tcccctgagt ggtatgacac
     8581 tgcccgatat aagcagcagt ttgagcgcac catagcatta agacggcggc aaaaaatagt
     8641 cgtagtaata acacgcattt ttctccaaag attatctgca tatggcatcc attcgcgtaa
     8701 tcgcagagtt aatttgttgt tcggtaagct ggtaactgat atgacaaatt tgatcgggag
     8761 cttcgcccca gcgtacagtt atcgctccct gagcgggcaa accggacata tagagcgagc
     8821 catcatcatc aataatgccg ctattagtct gtatgccgtc cagcgtcgct atcgcgccaa
     8881 aacgcagcgg tataccctgc tttgatgttt tcattaatac ccttgctccc gcatgggcat
     8941 tgaactccgc cagaaccaac gcccctttgg tgggaaccac gttgactact gcattttcaa
     9001 gatccacgtt acgttttaac gatgccgcat caagtgcgat tcgattttcc cgataagaag
     9061 tggcataggg gacaacggta tatccgcgcc agtcagtttt cagcccggtg ctgttcgcca
     9121 cacgagtatt ctctgcgcct ggtgctgcaa tcagcacgtt agtttccccc agcgattgcc
     9181 ccagggtaat gccctgtgaa tgggcaacta aactgcctga aagagcgtag ttcagttgtt
     9241 gttgactgcc gttatcgctg tagttgtagc ccactcgggc atctgcaaac gcccctttgt
     9301 agtccatgct ggcgctacca ttagccgttt tcccctcgct gttatatccc tgttgcacgc
     9361 tgtagctcag gttaccgtca tcgagtagtg tctccgttaa acccaggttt tgtgtaatgt
     9421 gcccttcgtt atcgatactg gtgttagagg tcatccaggc gtaatttttt ttcctggtat
     9481 agctatcatt acttctgggg agcaacagat tgagcggcag ggaaaaattt agtgcaaaca
     9541 cctgatcagc atcaggttga ccacgggact tactgtagtt ccaggaaacc cccagcgaga
     9601 gatctttaat ttgcgtgttg tagccaaact gtaatagccg atcctgttga tcggtatgcc
     9661 agtaagtttg ctggctacca cttaaataaa acgaaccgta ctcgcctaat tgctgggaga
     9721 tattgacctg cagtttgcca cgtttggtgt aaaacaaatt gtaataacgc gaccacatcg
     9781 gcgtatcttc atcatcaccg tcattaaatt cgtagccatc catatgttta tacatggtgt
     9841 cggaaagggt gtagaaaccc gacgtcgaat agcgatagcc catcaattga aaattagtgc
     9901 ccaattggtt gagcgttttg gcatacagaa aacgatatga ctgaccttta tgttcgcttt
     9961 ggtctgccag cgtactcttc gcctgggtcg catcgaacga aattgctccg aaatcgccaa
    10021 ggttaaatcc cagaccaaac atggcggcac ggtaatattc agcatattgt ccaccaccat
    10081 accatgtcgt tccccacggt ccgccccact gcaacgtggt ctgggcaaat ttgctctcct
    10141 gctgttcatt actattggtt ctgtatttcg ccagtgtcac cgcgtatttg attcgcccct
    10201 gacgctggag taatggcacg ctggaaaacg gtacgctgta gctattgacg ctgccgtccg
    10261 cttctttgat ttcaactaac aaatcaccgc tcgacgacgt ggaatagaga tcactaattt
    10321 caaaagcacc aggcgataca taagtttggt aaatggtgta accattttgc ttaatcgtta
    10381 tctgggcatt gcttttggcg ataccacgca ccacaggcgc aaaccctttc aggctgtctg
    10441 gcagcatgtt gtcatctgac gccagctgca caccacgaaa gctgacacta tcgaaaaaat
    10501 cgcctgcagt ataatcatca cctacggtca gttcgccttt tagtggaata accgcccgct
    10561 gcaaatagct gctgagattc ttccattctg cggtttggcc actactgcga ctccatgttg
    10621 aattgttgcg taatctccat gggcctaaat taacgccact gtttaaattc agaaaatagc
    10681 tgtcgccaga atcactgtct gcgctgctat gaatactgtt agccccgcta aacgaatatc
    10741 ccagtaatag cgcgttgatc ccttcatccc aacgttcagg ggggacataa ccacgggcat
    10801 tacttttgat ggcgatttgc ggtactgaaa tttccagacg gagtttattc acattaaacg
    10861 ttacgctggc atccggaata atctccgcca atggcacaca cacttgtttg tccagttgtt
    10921 taaaagccgg aaacgcatca gtattaacac ccattgcatc caggctttca gtcgtcaaac
    10981 aggcggcaat ccccttctct ggcgttatct caacaaaatt gacgttgcgc gtatctacaa
    11041 ttgtctgatt cagaacaata tcgacacgat aaacccctgg cgttattttt tgtccttttt
    11101 cgaaacgaga caaatcggcc accattgagg gatcatcttt gaaaaaagca ggatcgaacc
    11161 aggtttcagc ttgtgcattg aacgctgccg acacaaacac caccagccca ccaataaacg
    11221 atggcactcc accagagctt aacaggctgt gttgtcgatg agttctatac atctcgctct
    11281 cttaactgaa gacggcttac gatttacagt tgagttgcgg cgtttccgcc ccgtagtcat
    11341 tgatggttcg ata
//
LOCUS       DAJTMX010000122        11255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTMX010000122 DAJTMX010000000
VERSION     DAJTMX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.122"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO145_003274"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="OO145_003274"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5468288.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO145_003275"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="OO145_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5468289.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="OO145_003276"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="OO145_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5468290.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="OO145_003277"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="OO145_003277"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5468291.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="OO145_003278"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="OO145_003278"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5468292.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="OO145_003279"
     CDS             complement(5839..6879)
                     /locus_tag="OO145_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5468293.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="OO145_003280"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="OO145_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5468294.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="OO145_003281"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="OO145_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5468295.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="OO145_003282"
     CDS             7821..8261
                     /locus_tag="OO145_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5468296.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="OO145_003283"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="OO145_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5468297.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="OO145_003284"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="OO145_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5468298.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11255
                     /gene="rsxC"
                     /locus_tag="OO145_003285"
     CDS             9490..>11255
                     /gene="rsxC"
                     /locus_tag="OO145_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5468299.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2623 a         2897 c         2837 g         2898 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat
    11221 gcggaaccag aagaacaggt cgatccgcgc aaagc
//
LOCUS       DAJTMX010000123        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTMX010000123 DAJTMX010000000
VERSION     DAJTMX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.123"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO145_003286"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO145_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5468300.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO145_003287"
     tRNA            complement(621..695)
                     /locus_tag="OO145_003287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO145_003288"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO145_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO145_003289"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO145_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5468301.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO145_003290"
     CDS             complement(2932..3990)
                     /locus_tag="OO145_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468302.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO145_003291"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO145_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5468303.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO145_003292"
     CDS             6068..6352
                     /locus_tag="OO145_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5468304.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO145_003293"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO145_003293"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5468305.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO145_003294"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO145_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5468306.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO145_003295"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO145_003295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5468307.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2544 c         2748 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTMX010000124        11047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTMX010000124 DAJTMX010000000
VERSION     DAJTMX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.124"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            409..1239
                     /locus_tag="OO145_003296"
     CDS             409..1239
                     /locus_tag="OO145_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5468308.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO145_003297"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO145_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5468309.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO145_003298"
     CDS             complement(2368..3828)
                     /locus_tag="OO145_003298"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5468310.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO145_003299"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO145_003299"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5468311.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO145_003300"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO145_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5468312.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO145_003301"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO145_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5468313.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTPQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO145_003302"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO145_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5468314.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO145_003303"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO145_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5468315.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11047)
                     /gene="fimD"
                     /locus_tag="OO145_003304"
     CDS             complement(9005..>11047)
                     /gene="fimD"
                     /locus_tag="OO145_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5468316.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRYDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2683 a         2829 c         2683 g         2852 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaactagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcggtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcata acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccg
//
LOCUS       DAJTMX010000125        11025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTMX010000125 DAJTMX010000000
VERSION     DAJTMX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.125"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1347
                     /locus_tag="OO145_003305"
     CDS             <1..1347
                     /locus_tag="OO145_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5468317.1"
                     /translation="DIETIDWLEGFLKTFNGTIIFISHDRSFIRNMATRIVDLDRGKL
                     VTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKARRTRNEGRVRALK
                     AMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQLVKDFSAQVLRGDK
                     IALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRAELDPDKTVMDNLA
                     EGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLLLARLFLKPSNLLI
                     LDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECWIFEGGGKIGRYVG
                     GYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKLQRELEQLPQLLED
                     LEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFERWEYLEALKNGG"
     gene            1477..2730
                     /gene="pqiA"
                     /locus_tag="OO145_003306"
     CDS             1477..2730
                     /gene="pqiA"
                     /locus_tag="OO145_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5468318.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            2735..4375
                     /gene="pqiB"
                     /locus_tag="OO145_003307"
     CDS             2735..4375
                     /gene="pqiB"
                     /locus_tag="OO145_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5468319.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            4372..4935
                     /gene="pqiC"
                     /locus_tag="OO145_003308"
     CDS             4372..4935
                     /gene="pqiC"
                     /locus_tag="OO145_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5468320.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            5191..5358
                     /gene="rmf"
                     /locus_tag="OO145_003309"
     CDS             5191..5358
                     /gene="rmf"
                     /locus_tag="OO145_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5468321.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(5428..5946)
                     /gene="fabA"
                     /locus_tag="OO145_003310"
     CDS             complement(5428..5946)
                     /gene="fabA"
                     /locus_tag="OO145_003310"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5468322.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(6015..7775)
                     /locus_tag="OO145_003311"
     CDS             complement(6015..7775)
                     /locus_tag="OO145_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5468323.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            7960..8412
                     /gene="matP"
                     /locus_tag="OO145_003312"
     CDS             7960..8412
                     /gene="matP"
                     /locus_tag="OO145_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5468324.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(8488..9534)
                     /gene="ompA"
                     /locus_tag="OO145_003313"
     CDS             complement(8488..9534)
                     /gene="ompA"
                     /locus_tag="OO145_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5468325.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(9890..10399)
                     /gene="sulA"
                     /locus_tag="OO145_003314"
     CDS             complement(9890..10399)
                     /gene="sulA"
                     /locus_tag="OO145_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5468326.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            10618..>11025
                     /gene="sxy"
                     /locus_tag="OO145_003315"
     CDS             10618..>11025
                     /gene="sxy"
                     /locus_tag="OO145_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5468327.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         2815 a         2618 c         2879 g         2713 t
ORIGIN      
        1 gatatcgaaa ccatcgactg gctggagggg ttccttaaaa ccttcaatgg gacgattatt
       61 ttcatctccc acgaccgttc gtttatccgc aatatggcga cgcgcattgt tgatctcgat
      121 cgcggcaagc tggtgaccta tccagggaat tacgaccagt acctgcagga aaaagaagaa
      181 gccctgcgcg tggaagaact acaaaatgcc gagttcgatc tcaaactggc gcaggaagaa
      241 gtgtggattc gccaggggat caaagcacgc cgtacccgta atgaaggccg cgtacgcgcc
      301 ctgaaagcga tgcgtcgcga acgtggtgaa cgtcgcgaag tgatgggtac cgcaaagatg
      361 caggtggaag aggccagccg ctccggtaag atcgttttcg aaatggaaga cgtttgctac
      421 caggttaacg gtaagcaact ggtgaaagat ttttctgccc aggttctacg tggcgacaaa
      481 attgccctga ttggtccgaa tgggtgcggc aaaaccacgc tgctgaaact gatgctcggt
      541 cagcttcaag cggacagcgg gcgtattcac gttggcacca aactggaagt ggcttatttc
      601 gatcagcacc gcgcggaact ggatcccgat aaaacggtga tggataacct tgccgaaggt
      661 aagcaagagg tgatggttaa tggcaagcca cgccacgtat tgggctattt gcaggacttt
      721 ctgttccatc cgaaacgggc gatgacgcca gtacgtgcgt tttctggcgg tgagcggaac
      781 cgcttgctgc tggcgcgttt gttcctcaaa ccaagcaact tattgattct tgacgaaccg
      841 accaacgatc ttgatgtcga aacgctggaa ctgctggaag aactgatcga cagctatcag
      901 ggcacggtat tgctggttag ccacgatcgt cagtttgtcg ataacaccgt tacagaatgc
      961 tggatcttcg aaggcggcgg taaaattggt cgttatgttg gcggttatca tgatgcccgt
     1021 ggtcagcaag aacaatatgt ggcactcaaa cagcctgcgg tgaaaaaaag cgaagaagcc
     1081 gccgcgccaa aagcagaaac tgtaaaacgc agcagtagca aactaagcta taaattgcag
     1141 cgcgaactgg agcagctacc gcaattgctc gaagatctgg aggcgaagct ggaagcccta
     1201 cagacgcaag tggcggatgc ttccttcttc agtcagccgc atgagcagac gcaaaaagtg
     1261 cttgctgata tggctgctgc agagcaggag ctggagcaag cctttgaacg ctgggagtat
     1321 cttgaagcgt taaaaaatgg tggctgatcg caaaatagtc gatttaaaca gagacagctg
     1381 acaacggtaa aattgcctga tgaactgggt ttatcaggca tgaaaaccaa cactttgtta
     1441 gtcatctgta aaatagcgca tcattaagga gtaccaatgt gcgaacatca tcatgccgcg
     1501 aagcacatcc tgtgctcgca gtgtgacatg ctggtggcgt taccgcgcct tgagcatggt
     1561 cagaaagcgg catgtccccg gtgtggcaca acgttaaccg tggcgtggga tgcccctcgg
     1621 cagcgtccca ccgcctatgc gttggctgca ctgttcatgc tgttgctgtc caacttgttt
     1681 ccttttgtga atatgaacgt tgcaggagtt accagtgaaa ttacattact ggaaattccc
     1741 ggcgtgcttt tttctgagga ctacgccagc ctcggcacct ttttcctgtt gtttgtgcaa
     1801 ctggttcccg cgttttgtct gataaccatt ctgttactgg tgaatcgcgc ggaattaccg
     1861 gtccgtttaa aagagcaact ggcacgggtg ctttttcaac tcaaaacctg gggaatggcg
     1921 gagattttcc tcgcgggtgt gctggtcagt ttcgttaaac tgatggctta cggcagcatt
     1981 ggggtaggca gcagctttct cccctggtgt ttattttgtg tcctgcaact gcgcgctttt
     2041 cagtgcgttg atcgtcgctg gttatgggac gacatcgccc cgatgccaga actgcgccag
     2101 ccgctaaaac caggcgtcac ggggatacgt caggggctgc gttcgtgctc ctgttgtacg
     2161 gcaatccttc ctgctgatga acccgtgtgc ccgcgttgta gtaccaaagg gtacgttcgg
     2221 cgtagaaaca gcctgcagtg gacactcgcg ctgcttgtaa cgtccatcat gctgtatctt
     2281 ccggctaata ttttgcccat catggtgacg gatttattag gctcgaagat gccgtcgacg
     2341 attctcgctg gggtcattct gttatggagc gaaggctctt atcccgtcgc tgcggtgatc
     2401 tttctggcca gtattatggt gccaacgtta aagatgatcg ccatcgcgtg gctgtgttgg
     2461 gatgccaaag ggcatggcaa gcgcgacagt gaaagaatgc atttgattta tgaagttgtt
     2521 gagtttgtag gccgctggtc gatgattgac gttttcgtta tcgcggtgct ctcggcgctg
     2581 gtgcgtatgg gaggtttaat gagtatttat ccggcaatgg gtgcattaat gtttgcttta
     2641 gtcgtcataa tgacaatgtt ttctgctatg acgtttgacc cgcgtttgtc gtgggatcgt
     2701 caacctgaat cagagcatga ggagtcctga cagtatggaa tctaataatg gggaagccaa
     2761 aatccagaaa gtgaagaact ggtctcccgt gtggatattt cctatcgtca cggcgctcat
     2821 tggggcctgg gttctttttt atcattacag ccatcaggga ccggaagtga ccctgatcac
     2881 cgcgaatgcg gaaggaattg aaggtggcaa aaccaccatt aaaagccgta gcgttgacgt
     2941 cggcgtggtt gaaagcgcca cactggctga tgatttgacg cacgttgaaa tcaaagcgcg
     3001 gctgaattcc ggtatggaaa aattgctgca taaagacacc gtcttttggg tggtgaaacc
     3061 gcagattggt cgcgaaggga ttagcggcct gggaacgctg ctgtctggag tttatatcga
     3121 actgcagcca ggcgcgaaag gcagcaaaat ggataaatac gatttgctgg actcaccacc
     3181 gttggccccg cctgatgcga aaggtatccg tgtgattctc gatagcaaaa aagccgggca
     3241 gctctcgcca ggagatccgg tgctgttccg tggctatcgg gtaggttcgg ttgaaaccag
     3301 caccttcgat acgcaaaaac gcaatatcag ttatcaactg ttcatcaatg caccttatga
     3361 ccgactggtg accagcaatg ttcgcttctg gaaagatagt ggcattgcgg ttgatctgac
     3421 gtcagcgggg atgcgtgtgg agatgggctc attgacaacg ctgcttagtg gcggtgttag
     3481 ctttgatgtg ccggaaggtc tggatttagg gcagccagtg gcaccgaaaa cagctttcgt
     3541 tttgtatgat gatcagaaga gcattcagga ttcgttgtac accgatcaca ttgattatct
     3601 gatgttcttt aaagattcgg tacgcggtct gcaaccggga gctccggtag aattccgggg
     3661 tattcgcctg ggtaccgtaa gcaaagtgcc attctttgcg ccgaatatgc gtcagacatt
     3721 taacgatgat taccgtattc cggtactgat tcgtatcgag ccagagcggc tgaaaatgca
     3781 gcttggcgaa aatgcggatg ttgttgagca ccttggcgaa ttgttgaaac gtggtttacg
     3841 cggatcgctg aaaaccggaa acctggtcac tggtgcactg tatgttgatc tcgatttcta
     3901 tccaaatacg cctgcaataa ccggtattcg tgaatttaat ggttatcaga ttatcccgac
     3961 tgttagcggc ggcttggcgc aaatccagca acgactgatg gaagcgttgg ataagatcaa
     4021 caaactgcca ttgaatccga tgattgaaca ggcaaccagt acgctttctg aaagtcagcg
     4081 cacaatgaaa aacctgcaaa cgacgctgga tagcatgaac aagatcctcg ccagccagtc
     4141 gatgcagcag ttgccgacgg atatgcagtc aacgttgcgt gaattgaatc gcagcatgca
     4201 gggcttccag cctggctccg cagcctacaa caagatggtg gcggatatgc agcgccttga
     4261 tcaggtgttg cgagaactgc aaccggtgct gaaaacgctc aatgagaaga gtaacgcgct
     4321 ggtatttgaa gcgaaggaca aaaaagatcc agagccgaag agggcgaaac aatgaaaaag
     4381 tggctagtga cgattgcagc actgtggctg gccggatgca gctccggcga aattaataaa
     4441 aactattacc agttacctgt ggtgcagagc ggtacacaaa gtaccgccag ccagggcaat
     4501 cgtctgttat gggtagagca ggtcactgtt cctgactatc tggcggggaa tggtgtggtt
     4561 tatcaaacca gtgatgtgaa gtatgtgatt gccaacaaca acttgtgggc cagcccgttg
     4621 gatcaacagt tgcgcaacac cctggttgcc aacctgagta cgcaactgcc cggctgggtg
     4681 gttgcctccc agcctctggg aagcgcccag gacacgctca atgttaccgt aacggagttt
     4741 aacggtcgct atgatggcaa ggtcattgtc agtggtgagt ggctgttgaa ccaccaggga
     4801 caactgatca aacgtccgtt ccgtctggaa ggagtgcaaa ctcaggatgg ttacgatgag
     4861 atggttaaag tgctggccgg tgtctggagt caggaagccg cttctattgc acaagagata
     4921 aagcgtctac cttaattata aagatttgta aatataaccg tctccggtat gttgcctgag
     4981 gcggtttttt tgtttctaac gtgcggaaaa atttgttcct cttcacattt tttgtacaac
     5041 cgacatgccc gtgtagctca caaatatgac agtggcgtga attttgcgca ttgacggcag
     5101 ttatgattcg cggtattgct taactgtgat tgcacattta gtaatcactg ttttcttttc
     5161 caccagaaac cagtatgagg gaaacgaggc atgaagagac aaaaacgaga tcgcctggaa
     5221 cgggcacatc aacgtggtta tcaggccggc atcgccggac gctcaaaaga aatgtgtccc
     5281 tatcagacgc tgagtcaaag gtcacaatgg ctgggaggct ggcgagaagc catggcggac
     5341 agggtagtaa tggcctgatt ctgtctcttt aaaaagaaac ctccgcattg cggaggtttc
     5401 gccttttgat actctgtctg atgataatca gaaggcagac gtatcctgga acagaccgac
     5461 tttcaggtcg ctggcggtat agatcagacg acaatcaacc agcacttcgc catctgccag
     5521 acccataatc agacgacgat taacaatgcg tttaaagtga atacggtagg tcactttttt
     5581 cgctgtcggc agtacctgac cagtgaattt cacttcgcca acgcccagcg cgcggccttt
     5641 accttcgccg cccagccagc cgaggtagaa ccctaccagc tgccacattg cgtccaggcc
     5701 caggcatccc ggcataaccg gatcgccaat gaagtggcat ccgaagaacc acagatccgg
     5761 attgatatcc agttctgctt caacataccc tttgtcgaag ttaccacccg tttcggtcat
     5821 tttgaccaca cggtctatca tcagcatgtt cggtgctggc aattgcgggc ctttagcgcc
     5881 aaacagttca ccgtgaccag aggcaagaag gtcttctttt gtataggatt cgcgtttatc
     5941 taccatgttc tctgtaagcc ttattttatt gaagcacgca ggatagctaa cacgtgtacg
     6001 ctgaacaagt ccgatcagtt cggaataaac cagttcagcc aacgtaatgg ccatggaaaa
     6061 cggtgacgtc cttcctgttg cgatgcttgc gcgatacgtt cctggatggt ttgcatcagc
     6121 gtcgtttggc cttcgccatc ccacaccaga tttaataata acggcagtgc gtcagtcaca
     6181 tcgtctactg cccagatggt gaatttgcct tcttctaccg ctttcaccag ttcactgtga
     6241 agacttaaat ggcgaacgtt agcagtgggg ataatgacac cttgtttccc cgttaactca
     6301 cgttgctggc aaatagcaaa gaagccttcg attttctcat ttaaaccacc gaccggctgg
     6361 acgcggccga actgatcgac tgaacctgtg atagcgatac tctgattcac tggcacatca
     6421 gcgagggcgc ttatcagggc gcagagttca gccatcgagg cactatcgcc atccacttca
     6481 ctgtatgact gctcaaatgt cagcgatgct gagaagggga tctgttgctc aagctgtagt
     6541 tccgacatca ggaacgcctg catgatcatc atccctttcg catggatatt gccgccaagc
     6601 tccgctttgc gttcgatgtc ggtgaattca ccatcgccaa tatgcacaac gcagctaatg
     6661 cgagaaggtt cgccaaaagc gcgtggatga cccggaaatt caatgaccga aagggcgtta
     6721 atttgcccga tgcgttcgcc ttcggtttca atcaggattt gctcctgaag gatctcatcc
     6781 tgcatacgtt cagcgaggaa accttcgcgc cattcacgct gctgcagcat taagtttagc
     6841 tgctcgccgg agaaggtgtc gccatcacac agggaggcga cctctttaca ctggcggagg
     6901 atccactgcg ggctaagcgg aagtgtttct tgttcaccgg tgtagcgtgc tgcttcgcgg
     6961 ataagtaccg gccaagcatc cgctcccggg gcaggtaagt gattatgtct ggcggtaaat
     7021 gtcacccagc gacaccactg gcttactgac tccgcatcga caatctgcag agtatcttca
     7081 aattcgctat aaatagcctg ctctgaaagc tctggctcca tctcctggaa atcagccaat
     7141 gattcgcgtt cgcctaccag aatgactttc agcttcaatg gcattgaagg cacagagacg
     7201 gggagagggc gcgactcatc aaacgcaacc cagtcaaaac gctcgcggtt aacgatattt
     7261 ttcagccgca tccacagcag aggttgcgcc agcagtgtac gcaaagagat gatgagaata
     7321 ccgccatttg cctgatgcac cagaccaggc tgcagggtaa tgtcgccatt aaactggcgc
     7381 aggcagccaa agagttgctc cgcttctacc cagtcggcag cgacaacttg cgttaaagtc
     7441 gcaaaattat catctgcact cactgcgtga cgtaagcgga tggagtggcc ggaaacctca
     7501 tagtgatcgc ccaccagttg gcctgcatcg ctttgtagcg tacgcgcggc atcggcaata
     7561 agattcagat actcagactc ttccggggcc ttagccagca taaaagagga ggatgctcgc
     7621 gtatgcagca actgctccag cgcaaattgc agtcgcggtt gagtatcact gaataaagga
     7681 tcgttttcgt caatcaaatg tggctgagca aatatttcct gatagctatc ggtatcagga
     7741 accaggtcac gccatgcaag tttcgtaatg gtcaaagttg atgtttttta gtctgttgtc
     7801 aaagccgcga ttataccgta accggcacta cagcacacgt agaaagcacc gacaatactc
     7861 ctggctgggc gttaaagctc acaggatgga gattcttttc ttcactggcc taaaaagctg
     7921 atattctgta aagagttaca cggtaacatt gagatcgcta tgaaatatca acaacttgaa
     7981 aatcttgaaa gcggttggaa atggaagtat ctggtgaaga agcatcgcga aggggagtta
     8041 attacccgtt acatagaagc cagtgccgcc caggaagccg tcgatgtgtt gctctcgctc
     8101 gaaaatgaac ccgtgctggt aaatggctgg attgacaagc atatgaatcc ggaactggtc
     8161 aatcgcatga agcagaccat tcgggccaga cggaagcgcc attttaatgc ggaacaccag
     8221 catacgcgca aaaaatcgat cgatctggaa tttattgtct ggcaacgtct ggctggtctt
     8281 gcacagcgtc gcggtaaaac gctttctgaa acgattgttc agctgattga agatgcggaa
     8341 aacaaagaga aatacgcgaa taaaatgtct tctcttaagc aggatctgca ggcattgctg
     8401 ggtaaggaat aactgacgaa agtcagttca atttactaaa ggcaaaaaaa accccgcagc
     8461 agcggggttt ttctaccaga cgataactta agcctgcggc tgagttacaa cgtctttgat
     8521 acctttaact tcgatctcta cgcgacgatc cggagccaag cagtcgatca gtgcagcacg
     8581 ctgtttcacg ttgtcacagg tgttgccagt aaccgggttg gattcgccca taccacgtgc
     8641 ggagatcttg tctgccggga tacctttgga gatcaggtaa tcaacaacag actgagcacg
     8701 gcgctcggac agaccctggt tgtaagcgtc agaaccgatg cggtcagtgt aacccagaac
     8761 aactacggaa ccgtctttcg gatctaggtt gctcagctgg ctgtacagct gatccagagc
     8821 agcctgacct tccggtttca gggttgcttt gttgaagttg aacagaacgt cagacttcag
     8881 agtgaagtgc ttggtctgta cttccggtgc cggagctgga gcaactaccg gagctgcttc
     8941 gccctgaccg aaacggtagg aaacacccag gctcagcaga ccgttgtccg gacgagtacc
     9001 gatggtgttt gcgtcaccga tgttgttggt ccactggtat tccagacggg tagcgatttc
     9061 aggagtgatc gcgtactcaa caccacctgc gaaaaccgga gaaacgccag tgtcgtggtc
     9121 tttaaaggat gcgccaccag gtacgttagc cttggtgtct gcacgccata ccataccacc
     9181 cagacgagtg tagatgtcca gatcgtcagt gattgggtaa cccagtttag cggtcagctg
     9241 aacgccctga gctttgtatg cgccgttgat gttgtcgcct ttgtacggca tacgacctaa
     9301 ccagtcgtaa cccatttcaa aaccaacata cgggttaacc tggtaaccac caaaagcacc
     9361 tgcacccagt tggttttcgt gggtcggacc attgttagga ataaaaccag tgtcatggta
     9421 ctgggaccag cccagtttag caccagtgta ccaggtgtta tctttcggag cggcctgcgc
     9481 tacggtagcg aaaccagcca gtgccactgc aatcgcgata gctgtctttt tcattttttg
     9541 cgcctcgtta tcatccaaaa tacgccatga atatctccaa cgagataaca cggttaaatc
     9601 cttcaccggg ggatctgctc aatattaact ctaccgatat cttcggctta tgccgagcac
     9661 ccctggcgat gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac tccgtcaggc
     9721 atatgaaaaa aaagtcttgt ataaggtatg tttaatcttt ttgtcagcga caatttacag
     9781 aaaaaaaacg cggaaaccgc ttcagacaag ccttcgcaag gaaaattagt cacgactgaa
     9841 agcattggct gggcgacaaa aaaagttcta ggattaatcc taaatttact taatgataca
     9901 aattagagtg aatttttagc ccggaaagtt gtctcgttgt gtgagaggat gcgcttaccg
     9961 gacgcataat aaaccccata gcgttacctt catttgccgc atcaacaagt tcagcatgct
    10021 cttcttcagt caaatcatct gtcaaccaac cgatcaccac actgtaattg cccgtgcgta
    10081 aagcgcgaac cattgactcc actgtgtggc aaggggagag ctggctaatc tgcattactt
    10141 tcgttaaggg tagcccagat gcctgaaccc attcccgact cagtttttgt tgcggtgtta
    10201 accagagttg ccagcgcgat tgctgaccga gttgctgtaa caatggcaac agtagaagtt
    10261 gcgtcatcat gggctgatct tcgcgataga caacttcact gataagcccg gctgtagtgt
    10321 tttccgtaga gacacgcgca attttacttg ctgcggatga gaacgacgaa gaacgatgtg
    10381 catagcctga agtgtacata atcaatccag cccctgtgag ttactgtatg gatgtgcagt
    10441 acatccagtg acaacaaaga tcaaccctat tttcggaaag agcctcgcaa attttgtcgt
    10501 tggtgacggg aaaacataaa ttaatcttgc cccttaagaa taagttgcct attttcgtag
    10561 ttaacggatc cgttaatgtg aatcattctt ttatgttatg attttaaaag gaattttatg
    10621 aaaagcctct cctataagcg gatctataaa tcacaagaat acctggcaac gttgggcaca
    10681 attgaatacc gatcattgtt tggcagttac agcctgaccg ttgacgacac ggtgtttgcg
    10741 atggtttctg atggtgagtt gtatcttcgg gcttgtgagc aaagtgcaca gtactgtgta
    10801 aaacatccgc ctgtctggct gacatataaa aagtgtggcc gatccgttac cctcaattac
    10861 tatcgggttg atgaaagtct atggcgaaat caactgaagc tggtgcgtct gtcgaaatat
    10921 tctctcgatg cagcgctgaa agagaaaagc acgcgcaata cccgggaaag actgaaagat
    10981 ttgcccaata tgtcttttca tctggaagcg attctcgggg aggtg
//
LOCUS       DAJTMX010000126        10946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTMX010000126 DAJTMX010000000
VERSION     DAJTMX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.126"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO145_003316"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO145_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5468328.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO145_003317"
     CDS             complement(1433..1759)
                     /locus_tag="OO145_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5468329.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO145_003318"
     CDS             1894..2235
                     /locus_tag="OO145_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5468330.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO145_003319"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO145_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO145_003320"
     CDS             complement(2833..3543)
                     /locus_tag="OO145_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5468331.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO145_003321"
     CDS             3651..3956
                     /locus_tag="OO145_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5468332.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO145_003322"
     CDS             4155..4475
                     /locus_tag="OO145_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5468333.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO145_003323"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO145_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO145_003324"
     CDS             complement(6299..7288)
                     /locus_tag="OO145_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468334.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO145_003325"
     CDS             complement(7373..7864)
                     /locus_tag="OO145_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468335.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO145_003326"
     CDS             complement(8185..8685)
                     /locus_tag="OO145_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468336.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO145_003327"
     CDS             complement(8675..9190)
                     /locus_tag="OO145_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468337.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO145_003328"
     CDS             complement(9621..9989)
                     /locus_tag="OO145_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468338.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..>10946)
                     /locus_tag="OO145_003329"
     CDS             complement(10401..>10946)
                     /locus_tag="OO145_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5468339.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2809 a         2783 c         2706 g         2648 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggggcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca
    10861 gaccgagcgc cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg
    10921 tgagccgtgt gtcgtgtgaa ctgata
//
LOCUS       DAJTMX010000127        10930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTMX010000127 DAJTMX010000000
VERSION     DAJTMX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.127"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..206
                     /locus_tag="OO145_003330"
     CDS             <1..206
                     /locus_tag="OO145_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468340.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="OO145_003331"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="OO145_003331"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5468341.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2105..2821
                     /locus_tag="OO145_003332"
     CDS             2105..2821
                     /locus_tag="OO145_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5468342.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3277..3352)
                     /locus_tag="OO145_003333"
     tRNA            complement(3277..3352)
                     /locus_tag="OO145_003333"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3317..3319),aa:Asn,seq:gtt)
     gene            complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="OO145_003334"
                     /pseudo
     CDS             complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="OO145_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5085..5160
                     /locus_tag="OO145_003335"
     tRNA            5085..5160
                     /locus_tag="OO145_003335"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5118..5120,aa:Asn,seq:gtt)
     gene            complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="OO145_003336"
     CDS             complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="OO145_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5468343.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6250..7167)
                     /gene="nac"
                     /locus_tag="OO145_003337"
     CDS             complement(6250..7167)
                     /gene="nac"
                     /locus_tag="OO145_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5468344.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7495..7570
                     /locus_tag="OO145_003338"
     tRNA            7495..7570
                     /locus_tag="OO145_003338"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7528..7530,aa:Asn,seq:gtt)
     gene            complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="OO145_003339"
     CDS             complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="OO145_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5468345.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="OO145_003340"
     CDS             complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="OO145_003340"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5468346.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="OO145_003341"
     CDS             complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="OO145_003341"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5468347.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10454..>10930)
                     /gene="cobU"
                     /locus_tag="OO145_003342"
     CDS             complement(10454..>10930)
                     /gene="cobU"
                     /locus_tag="OO145_003342"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5468348.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3037 a         2778 c         2367 g         2748 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat
     1981 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2041 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2101 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2161 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
     2221 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2281 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2341 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
     2401 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2461 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
     2521 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
     2581 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa
     2641 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2701 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2761 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2821 attatatttt aaagaaatct gtctttatgg cagatttctt taatctcata taattcttat
     2881 aaaaaatata atatttaact cgtcatattg cttataaaaa atccccgtcc ccagagaaat
     2941 aatatttatt aaaatggcag gtcttctttt tctgattacc gaagacaaag tggcaattac
     3001 gtatagtttc ccgataaaga cgcgacagct acgtcccgca taaggcattt ttctctttat
     3061 ctttgtacgg tacttcatgg aacagaattt ttgaccttgt gaatcgtgat atctgttggg
     3121 gaggggcaat ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca
     3181 aaaaacccgg ccactttttt acaaagtggc cgggtaaaaa taatttttat tttttaactg
     3241 tcctgagtct catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac
     3301 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
     3361 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
     3421 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
     3481 cacttttgcg gctcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag
     3541 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca
     3601 ccccagccaa gcatgattcc ctgcacataa ccgactacaa cccggcaacc ccacatgctc
     3661 aacatagaaa cccacatggc gtaacgggca tcacgagcac ccttaaatcc agcgggtagc
     3721 acccatgagg cggaccaaat aggcataaat aaagcattta gctaaatcag aatcacaacg
     3781 acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
     3841 gttagccagg cgatggccgt taatccaaga gtggacagcc agaacacatg ccgcaactga
     3901 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
     3961 gaaccgagcg cacttccggg taagttgata agagccgcaa ttgagaacgc gataaaattt
     4021 ccggcaataa cgctggtccc catcccggca acgaacattt gggttaataa ccgaccactg
     4081 gtaaataaca ctgattcgac actcgctgga ataccaatcc ccatgacttc ccagataatg
     4141 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
     4201 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccaac
     4261 ccagccccga ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa
     4321 tattcaggct accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc
     4381 cgctaccaat aagagtgatg gcagcggctg gataactgag tacggtcagc tccagataag
     4441 tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt
     4501 cgccaaaatg atgaattagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg
     4561 actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgctacaa
     4621 cgacagtagt accaagatcg atagctgcaa aaaaagccat aatgaccata ttgaagctgt
     4681 ccgccaaccc cacgccggcc atcgcatctt ttcccagcca actgaccaga aaagtgctca
     4741 ggacccccat caacaggaca caggcgttct ccatgaagat aggaacagca agcggggtta
     4801 tctcgcgcta gaacaacact ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa
     4861 caacctggcg taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc
     4921 acatttcaaa tgatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc
     4981 tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt
     5041 ttgacaaaat atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt
     5101 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     5161 aatttctgaa aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct
     5221 cttcactgtt ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa
     5281 gttccagaaa gcgccaaaca tagttacgct gaagttgtcc tcgcttcagt cccaaccaga
     5341 cagtattagc atcgaaaaga tgccgcgtat ccaggcggat taaattctct tcctcttgtt
     5401 cgccgctgga ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa
     5461 tgacatcaga atcctgtgca cttaatacaa tatctgccgg caaacctttg cgggtaaatg
     5521 cgtcatcagt gcgtgagcgc cccgtaattc cctgtcggta agtaattaac ggccacttcg
     5581 ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca
     5641 aactatggtg ccaacaaaac catgggaagg cgacgagctg cgggtcatta ctcaaacgct
     5701 cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg
     5761 aaccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct
     5821 ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat
     5881 cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg
     5941 caatgaccag taatgctttg cccggttcag tcatccccag cagtcgctta cctcgtcgaa
     6001 caaatatttc gatgccaaat tcatcctcca gttcccgaat atgacggcta acgcctgact
     6061 gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc
     6121 ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta
     6181 ttgttaaagt ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg
     6241 atataacgct tagctcacca attgccactg ccttttttcc atcactggag aactaatcac
     6301 tgacattaac aactctttca ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa
     6361 atttaaggac aaagagagac tcattgaagg agtggtaatg cgtgacatcc acccatttac
     6421 tgcgccacat aacgaacgcg cagccgattc aggtaatact gcaacgccca tgccgctggc
     6481 aatcgctgcg gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg
     6541 ccgtagggaa aaagcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa
     6601 aaggttcatt tgcgcaatag cattaacatc aacgttttgc cccgggcaat cttgagttcc
     6661 taccaggaaa agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg
     6721 ctcataaatc accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc
     6781 actattttca tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa
     6841 gggcatggtg atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga
     6901 taatgactga ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc
     6961 ccgtgcatgg gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg
     7021 aatcaaaagc tgttgattta actcaccttc cagtatggca acctgttggc tgagcgctgg
     7081 ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta ccaatatcta caatttttac
     7141 gaagtatttc aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg
     7201 ccgccattac ttacaaccag tatgcaagat gcctgccagt tctattttgg cgttgatgcg
     7261 caagctaacc aactgtcaaa taagagatta tgatagattc gtcattggcc ctttttatca
     7321 gcagtcgcat tcacctgccc tataaaagga gggtatgcac cacgatggtt cattacccaa
     7381 taagattgaa agctcaccac tttgttgaaa ttggcagcaa acaaacaaaa aaaatgcatt
     7441 tcaccctttg acatcaccat gcactgccat taatatgcgc cccgttcaca cgattcctct
     7501 gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc
     7561 agaggagcca aattcaaaaa agcctgcttt cgagcaggct ttttactttc taattaccaa
     7621 cgctcttaaa acatctgtct tgaaccagaa ctaatttgca caggcattcc cgatcgatgt
     7681 tgcaacgcag catttgcgcg atttacatca acttcttgcc cgttgataaa cgcccgcaaa
     7741 gatggggtta ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac
     7801 agcggctcat gaacttccag ccactttgaa ccatccggtt ccgttgtgta tttcactggc
     7861 tgatcaataa tttgtacacg agtgcctaca ggaacattat caaacagata tttgatatcg
     7921 tcattacgca gacgaataca gccctgactt acacggagcc caataccaaa attggcattg
     7981 gtaccgtgga tggcatacaa cctgccaata taaatcgcgt acagccccat ggggttatcg
     8041 ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccggatg
     8101 ttcggcgttg gcgtccaggt tggtgcttct tgtttacgtt caacggtagt cacccagtta
     8161 cgcggggttt ctcgcccagc ctggccgata ccaataggaa agacttccac tgtattactg
     8221 tctggcggat agtaataaag acgcatttca gcgacgttaa ccacaatccc tttacgaaca
     8281 gtggcgggca aaatcagttg ctgcggaatg gtgagttgtg agccaggctt cggcaaaaaa
     8341 acatcagcgc ccggattcgc ttccagcata ttacttaacc cttgcccgta ttgtgcggca
     8401 aaagtctcca gcggctgagt attgtgatca ggaacagtta cagtaagcga ctgccccact
     8461 aaacggctac cctctggagg taatggataa gttaccgcca ggctattttg gctggcaaaa
     8521 agcagagcaa atgagcaaag aatatttaca cgacgcatca tgtccctttc ctaagtcgcg
     8581 aatactatcc gataagtata gcttttatca gacttttcgt ttttagctgt tcaaatcaga
     8641 agtcgtattc cccggtagaa caatattact ggcagcaagt tcgcccatgt tgttgtatat
     8701 cgcacaagca gcttcgatga tggacatcgc cagagctgca ccactcccct cacctaaacg
     8761 catatccata ttgagataag gctccagccc caaatgcgag agcgctatac gcgcgccttt
     8821 ttctgccgac aagtgagaag gaatgagata cggtttgatt gcaggagaca tctggcaggc
     8881 tgcgagcgca gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc
     8941 agcacctaac atcactccag ctattccgac caaatcaaat ccacccactt ttgccaggac
     9001 atcaacacca tcctgaggat ttggttgatt caacgtaatc gcccgacgca caacatcaat
     9061 tttattagcc agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc
     9121 ccggccagtg attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc
     9181 acctacacca aacagcgtga caccgttttt cgccagctcc cgcgtataac atatgacgtc
     9241 caaaagcaac ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct
     9301 acctcgtgcg acacgcatgt tgataagccc ggggataggc tcagcagtat caataccaac
     9361 atcaattacg tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc
     9421 acgggtcata ttttcagcct gtatggctgt cacttctttt ggagaaatag cgaccccttc
     9481 ctcccagacg ccgtgatcgg cacacataac cagtaccgct tttttgccca catgcggtat
     9541 gccattcacc cccggcattc ctgccagttg tatggcaagc gcctccagct ttcctaggct
     9601 accaacaggt ttgaataacc cgtcaacatg ccgttgtgca cgcgacatag cggcagaatt
     9661 gatggctggg atcgtattca gtaaatcggc aattatttgc atgtcacgtt tctttatagc
     9721 agtgccagca ggaagaccag ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt
     9781 tgcccgccca gcgtacgttt gagaagctga ccgaggatga aaatcgccac catcgtcacc
     9841 accatagcag ccacaccatg catacccagc aataatacgg cagcaaaaat agcggctaag
     9901 ccgagtgtga cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct
     9961 tcttcccgcg cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca
    10021 catgctgccg ccagcgatgc aaggatcggt tcgccacgca gagccaactc gcttaacacc
    10081 agaatctttg ccagtaccac aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta
    10141 tcacgcatga tttccaacat gcgatcgcga ctacgtgcag aaaatacgcc atcacaggta
    10201 tcagcgaggc cgtccagatg gaattccccg gtcatcagcg ccagcacaag tacgctaaac
    10261 agtgccgcca gtggtgcgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc
    10321 gcgccaagca ataatccaat caaaggaaaa gtaataatac cgcgagaata atgttcgaaa
    10381 tccagtccct gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc
    10441 caaaataatt tgctcatttg atttttactc caatacccga aaccaccagc catacttcat
    10501 ttgccgcagc ggccaactgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca
    10561 gacgactctc cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg
    10621 ggcaacgttg gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg
    10681 cctgataatc ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca
    10741 ccattgtggt aacgcattcc agcaacacag cctcattcgg gttaatgtct gcatgaatta
    10801 attcatcaag atgttgccag cgctccactg tgcgccagtg ctccgggcgg ctttgccgat
    10861 gatgttctat ccgtgcagcc atctcatcat caaggatttg cgaggtagcg atatacagaa
    10921 cctgtgaaga
//
LOCUS       DAJTMX010000128        10926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTMX010000128 DAJTMX010000000
VERSION     DAJTMX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.128"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            154..504
                     /locus_tag="OO145_003343"
     CDS             154..504
                     /locus_tag="OO145_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5468349.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO145_003344"
     CDS             557..952
                     /locus_tag="OO145_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5468350.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO145_003345"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO145_003345"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5468351.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO145_003346"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO145_003346"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5468352.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO145_003347"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO145_003347"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5468353.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO145_003348"
     CDS             complement(5323..6423)
                     /locus_tag="OO145_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5468354.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO145_003349"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO145_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5468355.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO145_003350"
     CDS             complement(7571..8137)
                     /locus_tag="OO145_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5468356.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO145_003351"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO145_003351"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5468357.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OO145_003352"
     CDS             10263..10751
                     /locus_tag="OO145_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5468358.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJTMX010000129        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTMX010000129 DAJTMX010000000
VERSION     DAJTMX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.129"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO145_003353"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO145_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5468359.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO145_003354"
     CDS             214..402
                     /locus_tag="OO145_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5468360.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO145_003355"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO145_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5468361.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO145_003356"
     CDS             597..770
                     /locus_tag="OO145_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5468362.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO145_003357"
     CDS             complement(819..1892)
                     /locus_tag="OO145_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5468363.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO145_003358"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO145_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO145_003359"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO145_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5468364.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO145_003360"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO145_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5468365.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO145_003361"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO145_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5468366.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO145_003362"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO145_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO145_003363"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO145_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5468367.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2524 a         2685 c         2787 g         2468 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattt ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTMX010000130        10370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTMX010000130 DAJTMX010000000
VERSION     DAJTMX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.130"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..270)
                     /locus_tag="OO145_003364"
     CDS             complement(<1..270)
                     /locus_tag="OO145_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5468368.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO145_003365"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO145_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO145_003366"
     CDS             complement(1130..1336)
                     /locus_tag="OO145_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5468369.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO145_003367"
     CDS             complement(1443..2006)
                     /locus_tag="OO145_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5468370.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OO145_003368"
     CDS             complement(3381..5216)
                     /locus_tag="OO145_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468371.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OO145_003369"
     CDS             5317..5604
                     /locus_tag="OO145_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5468372.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OO145_003370"
     CDS             5576..7105
                     /locus_tag="OO145_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5468373.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OO145_003371"
     CDS             complement(7275..8492)
                     /locus_tag="OO145_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5468374.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="OO145_003372"
     CDS             complement(8859..9845)
                     /locus_tag="OO145_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468375.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10370)
                     /locus_tag="OO145_003373"
                     /pseudo
     CDS             complement(9842..10370)
                     /locus_tag="OO145_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3231 a         2371 c         1949 g         2819 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc
//
LOCUS       DAJTMX010000131        10367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTMX010000131 DAJTMX010000000
VERSION     DAJTMX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.131"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..475
                     /locus_tag="OO145_003374"
                     /pseudo
     CDS             1..475
                     /locus_tag="OO145_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            558..815
                     /locus_tag="OO145_003375"
     CDS             558..815
                     /locus_tag="OO145_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468376.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1373..2140)
                     /gene="fecE"
                     /locus_tag="OO145_003376"
     CDS             complement(1373..2140)
                     /gene="fecE"
                     /locus_tag="OO145_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5468377.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2141..3097)
                     /gene="fecD"
                     /locus_tag="OO145_003377"
     CDS             complement(2141..3097)
                     /gene="fecD"
                     /locus_tag="OO145_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5468378.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3094..4092)
                     /gene="fecC"
                     /locus_tag="OO145_003378"
     CDS             complement(3094..4092)
                     /gene="fecC"
                     /locus_tag="OO145_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5468379.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4089..4991)
                     /gene="fecB"
                     /locus_tag="OO145_003379"
     CDS             complement(4089..4991)
                     /gene="fecB"
                     /locus_tag="OO145_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5468380.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(5036..7360)
                     /gene="fecA"
                     /locus_tag="OO145_003380"
     CDS             complement(5036..7360)
                     /gene="fecA"
                     /locus_tag="OO145_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5468381.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7447..8400)
                     /gene="fecR"
                     /locus_tag="OO145_003381"
     CDS             complement(7447..8400)
                     /gene="fecR"
                     /locus_tag="OO145_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5468382.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8397..8918)
                     /gene="fecI"
                     /locus_tag="OO145_003382"
     CDS             complement(8397..8918)
                     /gene="fecI"
                     /locus_tag="OO145_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5468383.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9216..9398
                     /locus_tag="OO145_003383"
                     /pseudo
     CDS             9216..9398
                     /locus_tag="OO145_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9392..9496
                     /locus_tag="OO145_003384"
                     /pseudo
     CDS             9392..9496
                     /locus_tag="OO145_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9836..10093
                     /locus_tag="OO145_003385"
     CDS             9836..10093
                     /locus_tag="OO145_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5468384.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2413 a         2755 c         2847 g         2352 t
ORIGIN      
        1 ggacgggtaa gcgctgggcg tacctcgccg ttgttctcga cctgttcgca agaaaaccag
       61 tgggctgggc catctcgttc tcgccggaca gcaggcttac catgaaagca ctggaaatgg
      121 catgggaaac ccgtggtaag cccgtcgggg tgatgttcca agcgatcaag gcagtcatta
      181 tacgagcagg cagttccggc agttactgtg gcgataccag atcagacaga gtatgagccg
      241 gcgtggaaac tgctgggata acagcccaat ggagcgcttc ttcaggagtc tgaagaacta
      301 atgggtgcca gcgacgggct atgtaagctt cagcgatgca gctcacgcaa taacggacta
      361 tatcgttgga tattacagcg cactaagacc gcacgaatat aatggtgggt taccaccaaa
      421 cgaatcagaa aaccgatact ggaaaaactc taacgcggag gccagtttta gttgaccaca
      481 acagactacc tgaagggagc cgcggtcgcc tagcagttgc agtagcagga gatcatccag
      541 ccgcagtaca ggtcacgatg actctggtta atgataccgg ctttgacccc gtattttccg
      601 gctctatcgc tgaatcatgg cgtcagcagc cgtgcacacc atcctattgt tgtgactggg
      661 aggctgccac catgcttcgc gctttccctc tggcgaaaaa gggagaagga cgggcccgtc
      721 tgccttcact ttatgccagc ttcggtaagc tgggtgagac accgactcat gaagatatca
      781 ttgataacaa tcgatccatc aactggcctg tataacgtgg ctgccggtga ttaagaaagc
      841 tgcacctacc taagtagtag caaacgcaca ctttttagaa aaatcgatgg tcagaaactg
      901 gattagcaat tccgttccag ggttgctttt gatttacgtt ggcgtctgat cattgattta
      961 tcctcaaaag cccaacctca ttggtaatga accagctccg tgaatgtccg ctctggcaca
     1021 gagcgaaatt ttttgatctc cccccctgaa atctaaactt agtcatgtca cgtttttggg
     1081 tttctaaaat tttaacttcg cgtttttcgt tgccgtaagg gttatacaga aatgtccgtt
     1141 aagcagagtt caaaattgat tgccgtgatc acgactggtt tgaaagccgc gcccaagcct
     1201 gtacagctct ggtttgcgtt gattatgaac ctgtcagcct aaagcaagcg gatggacgat
     1261 gagtattggt aatctttcag agtccggaaa agttcagccc cagtctgaac aggcttgctg
     1321 gcgccagtcc agtttcattc agtcgtggtt tggttcttac ggcctgtgca atctacctca
     1381 ttaggcacat cggcctgcca gataccggct cggggtgtat ttccgcttcc acgctgaata
     1441 ctgttctcag caatcctggg gtcatcacct cttctggtgt gccttgcgcc ataacatgtc
     1501 cgtttgccat taccaccagt tgatcgcagt accggctagc ctgattaagg tcgtgcagca
     1561 cagcgaccac cgttttcccc tgagtccgga gttcgcccat caaccgcatc aggtccacct
     1621 ggtgattgat atcaagatag gtggttggct catcaagtaa tacaacgggc gtattctggg
     1681 ccaggaccat cgccagaaat gcgcgctggc gctgaccgcc ggaaagctcg gttaaccgac
     1741 gaacggcaag atgattgatc cgggtctggt tcatggcgac attaactcgt gcattgtctt
     1801 cagcggagag acgcccccag agtgacagcc agggattacg accatacgaa accagctcct
     1861 ggactgtgat cccctctggc gttaaatggt gctgaggcag cagcgaaagc ctgcgggcca
     1921 actggcgtga tgagagcata tttatgggat tatcgccgag aaatacggtg ccagactgcg
     1981 gcattaaaag ccgcgaaaaa cagtttaaca gcgtcgattt cccgcaaccg ttagggccga
     2041 tcagggcggt gatcttcccc gttggcagtg agagtgaaac gtcgttaagt accttgtctg
     2101 tcccgtaact gaccgtcaga ttttcagttc gtaaagtcat ttatcgcatt ctcacaagca
     2161 accagacaaa ccacggcgca ccgataatgg cggtcagcac gccaaccggg agctccagtg
     2221 ggggatgaat aattctcgcc agcagatcgg caaccaccaa cagcaacgca cctgtcaggg
     2281 ctgaaacagg cagcagtctg cggtgacgtc caccggtgat gctacgcatc atatgcggca
     2341 ccacgagacc aataaagcta atcgggccgc aggcggccac gccggtagat gtcatggcga
     2401 cagctagtaa caaagcccag aatcgggtat ggggcaccga cacaccgagc gtggtggcgc
     2461 gcgcatcgcc gagtgcaagg aggtcgagat cgcggcaaaa actcaggctc agcggcagaa
     2521 ataaaatcat cagcgggatg gcaatcttca caaagctcca gtcacggccc cataagctgc
     2581 cggtcagcca cagcagggcg ttgttcacat cctgcgggcg cgagagcatc agataatccg
     2641 tcaggctggc ccagcatgca gaaagcgcca cgccggtgag cgccagcttc atcggctggt
     2701 gggtctttgc cagcatcttc agtaatatca accccgccat gccgcccgca aaggccagca
     2761 gcggcagcac catcacgggc agtgacggca taagaagtag agcccccaca gaggccaggc
     2821 tggcggcatg gttaacgccg agaatatccg gtgatgccag agggttgcgc acaatcccct
     2881 gtatcagcac gcccgccacg gcgagggctg caccgacaaa cagtgccagc agcaagcgcg
     2941 gcagtcggta ctccatcaat acataataat gctcgcgtcc ggcctgccag tcggtcagca
     3001 gcgcgcgcca cggcacgggg atcactccca tatggagtga taacagcgca cagcccgcca
     3061 gggcaagggt gatgaaaata accagcgcaa ttttcatcct cgcctcctca caagccagac
     3121 aaagcaaggg ctgccaatca gcgccagcac tgcgcctgcg ggcagatctc cggggaaggc
     3181 cagcgcgcgt gcgagtacat ctgccagcag catcagcgtg gcccccagca gcatgctcac
     3241 tggcagtacg ttgcgctgat cgaagcctgc ccagaagcgc gccagatgtg gcaccagcag
     3301 accgataaac gccaccggac ctgccacgct gacgcacgca ccaaccagaa gcagcactaa
     3361 catattgatg accaaacgta gcctcgtcag gttcactccc agcgtatggg cggtgctgtc
     3421 gctgaggttg agcaggttca gttgattcgc cagcagtaac acgacaggga ctgcagtgac
     3481 caccaccggc aagagctgcc agacatcctg ccagcgggcg tgggacactc cgcctgccag
     3541 ccagtaaaag atgccgtaag catgatcttc ggccagcagc agggtgatgc gggtaaggcc
     3601 catacaaaag gccgacagcg cgatacccgc gaggatcagt ttgtttctgt catgggtatg
     3661 acgaaatccg cctcctgcgg tcatgaccag cagccagctc acgccgcccc cgcatgccgc
     3721 gatgaacgac agagaatagc ctgcaatcgg cgtcggactc agcgcgctgg taagcgccat
     3781 agccagcgcc gcgccgctgt taatgccgag cagtgaagga gaggccattg ggttgtgggt
     3841 cagggtttgc agcagcgtgc ccgcgagcgc caggcttgcg ccgatcagaa cggcgaccag
     3901 gcttcgtggc aaacgaaggt tttgcaccag cgcttctggt agcgttggcg tgtgtccagg
     3961 cagcagggcg cgggttgcat ctgctccgga aacaggaatg gccgagtagc aaaacagact
     4021 cagccagaaa ataataataa gtgctgcaac gggaagcccc cacagcagca tcgggtgttt
     4081 tatcgcggtc atttcacaac ggtaagcggc tgatggtgga agatttttac cgtgtcagcg
     4141 gcaatacgct ctgcagcaaa aataccgcgc atccgcgccc aggtgttact gtcgaccgaa
     4201 gcaacctgct gcttctgcgc ggcggttaac atctgccaga gcggatcttg ttgccagcgt
     4261 ttaacaatgc tctcttcgcg atagtgggca accagcagcc aggcaggatt gaccgccagc
     4321 agttgctcca ggccgatgga cggcatggac gaacccgcca tcgcagcggg aacgttcagc
     4381 cccagagagg ccagcacgct gccggtccag gtctcctgag tatgcaggtt gaattgctgt
     4441 tcgcgtgatg tgccaaaggc cacgcgtgtc cctttgggaa gctggctggc ccactgcgcc
     4501 atcctctctt tatgttgttc cagacgtgcc tgcatctctc gctttttacc caccatttcg
     4561 ccgatgatag ccgcagattg caaattttca gcgtaggttt cgttgcggga cttaagcagc
     4621 agtaccggcg cgatttgctg caaggcgatg taaacccccg catggcgact gctgtcggca
     4681 atgatcaggt ctggtttcag agcggcaatg gcttccaggc tcggctgcgc gcgcgttccg
     4741 acggactgcc acggtttcag gtgcgcacgc acttcgggca ggatgcgttt tgcatcgtta
     4801 tcgtcggcaa taccgatcgg gctgacgtcc acggcggcca gcgcatcggc gaacgagagt
     4861 tccagcacca caatccgttg tggcgttttt tcgagtgtaa acgtgccgtg ttcgtcctga
     4921 accatggcgg caaaggcgtg gctgatcacc agcagcaggc ctgcaaaaag aaaacggata
     4981 aatgccaaca taatcacatt ccagctaaaa gcccggcaag ccgggcgtta acacatcaga
     5041 acttcaacga cccctgcata tacagcgtgc gcggctggcc tgcatagatg cctttgttgt
     5101 tgtcgtcata agagcggatg aagtagtcct ggtcgaagat gtttttcaca ccgaatgcca
     5161 ggttcagatc tgccatctgc gggccaaagt catacgccac gcgtgcaccc cagagcatga
     5221 agccgggaat gcggccggta ctgccgtcgg cgctctcttt caccgtattg gcgttatccg
     5281 caaactggct ggactggaaa tcgctgttca gattgaacgt ccagtttcct ggcttgtagt
     5341 ccacgcccag cgtgccttta tgtttcgggg agaatggtac cagattgccg taggtgtcgc
     5401 ctttctcgcg gatttccgcg ttcacatacg catagctggc gtagatggaa acgttatcaa
     5461 gcgttggcgt tagcgtaccc agatcgtaac gtgcctgcgt ttccagcccg gtatggcgcg
     5521 ttttgccacg tgcagtgacg gtgtcgttgg tctggttgga gtcgtactga ttgttaaagt
     5581 taatcaggaa cagccccatt tccgccgtca gcgcgccgtc gtcgtagcgg gtaccgagtt
     5641 cccaggttcg cgctttttcc ggttccacat tgccgctttg cacagccttg ccaatctggc
     5701 tgtactgtac ggtgccgaac gagccttcag tgtttgcata aagattccag ctgtcagtca
     5761 ggtgatagag cacgttcaac gccggaagcg gtgcgttata gctcacttct tcgtgcgtgc
     5821 ctgtgatggc gttgttctgg tatgactcga tatgttcgaa acgcataccc ggcgtgatgg
     5881 tccagttgcc gatgtcgatt ttgtcatcca gataccaggc gtgcgcctcg gtgccggaac
     5941 gcgtatcgcg gtcgtaaggg cttgagccgg acggcaactg cccgctgctg gtggcggtgt
     6001 agtaacgcat ttcatgcgtt gattcattca catagcgata gcccacgccc acttcgtgcg
     6061 cggaagggcc gatcataaag atctggctgt agcgtggctc aataccgcgc acccagtagt
     6121 tacgcggcga gagggtgatg cgtttgcctt gctccaggta gccgctgcgc agggtttggg
     6181 tgtagaaccc ctgaatgttg aatttatgct ggctgtctgg ctggaactgg tagcccaggc
     6241 tcgccagctt gcgacgaccc cagaagcggt catacgggcg ggtggattgc cagcgatcgg
     6301 cgtcgtaatc cgcgcgagac aggccaccgg gcatgtcggc ttcaccgtcg taatattgca
     6361 gcaggctgtt gaaggtgtgc acctcatccg gcgcatattt gcttttcagc atcaggtcgt
     6421 cgatgcgggt ggcgctgtgc tcgcgccagt cactgccgcg cgtgccggag tagagcagcg
     6481 cggtgccaaa accgttgtcc gctgtgccgc ccaccatcag gttgtgcgtc tctttcgggt
     6541 tgttttgtga agaggttggg ctgagctgac cttccacgcc cgcctcgata ccaaagtcct
     6601 gcggaatggc acgggtaaca aagttcacca cgccgcccac gctctgcggt ccgtaacgca
     6661 ccgcaccacc accgcgtacc acgtcaatgg catccatgtt gccgagcgaa atgggagcca
     6721 gtgaaagctg cggctgaccg taaggggcga aggggacggg gatgccgtcc atcaggacgg
     6781 tcgagcggct ggcgaggcgc gggttcaggc cccggatgcc aaagttcatc gccaggtcgt
     6841 ggctgccggt gccgttgttt tccggcgcgc tgacgccagg gatgcggtta agtacctcac
     6901 gcatggtggt tgcgccggtt ttggcgaaat cctcacggcg gatcacgtca cgcgcgccag
     6961 catgttcaaa tacgtcgttt tcacgcgcat cacccagcca gtcgccgacc acggtcaggg
     7021 catcttcttt tggtgcgggc gcgggctcca gcgtccagct gttatttccc agcggtttta
     7081 cctgcagtcc gctgccgtcc agcagttgtt gcaggccgct ctcgacgtcg taatcgccgt
     7141 gcaggccgtt gctctgcttg ccgcgcgtca ggctggcgtc aaccgagagg gtaaatccgc
     7201 tgtgtgcggc atactgattg agcgctttat cgagcgatcc cggtgcgata ttaacctgtg
     7261 cagcaaaagc ggaaaacgag agaccggcca gcggcagcag gctcaggcga atggcgttaa
     7321 ccaaaggtgt tgttttacga aaaacgcgta acggcgtcat accttcccca tcatcatttt
     7381 tgttgtgttc agctatgagt cgaacgagaa gggtaaaaag gacaatcgaa ataagaatta
     7441 ttttccttac agtggtgaaa tgtttatcca gtaccgcgta atagactgaa ttttaacggg
     7501 aagcgtttgc gcgataacgt tcaggatcgc atcggtattt ttcagcggga acgtcccgct
     7561 caggcgcagc ccggcaacgg cgggatcgca gcgcagcacg ccgttgcggt aacgggttag
     7621 cgtggctatc acctcaccca gcggtttatc gctgaagctc aggatgtcct tcgtccagct
     7681 tgtactctcg tcatccagcg gtttcactgc gccaaactca gaggcgctga actgcaggct
     7741 ttcaccagcg ttcacgatgc gtttttgcgc gggggcactg gcgagaagca cttccacagc
     7801 gtgctgctga acgtcaagct gcgtgaaatt atcctgctgg cggacggtaa attctgtccc
     7861 taaagcagtg agctggccct gacgggtcag gacacggaag gggcgttgca gggcatcttt
     7921 cgcggtagta atggcgattt caccgtacca gagccggacg gtgcgctgat gcgcatcaaa
     7981 acgcacatcc gccgcgcttt gggtattcag cgtgagcagg gagccatctt ccagttgctg
     8041 acggctgact gtgcctttgg cggtgcggta atctgcccgc agaccttcgc cggtttccga
     8101 ctgccagagc tgccagcctc cgccagcgcc gagcaacagc agtaatcctt tcatcacgtg
     8161 acggcgggtg aggcgggtat cgtgcaacgc ccggctggca acgtcgccag gcacaccgcc
     8221 aagctggttg cgcaggtttt caacctgctg ccaggcccac tggttatcct gatcctgttc
     8281 ataccactgt tgccagcgcg cttcctgttg tgggctgacg cgctcgccgc ttagcacggc
     8341 ataccagtgg gaagctgaac gcagcgcctg acggcgggaa tcggttaaca aaggattcat
     8401 aacccatact ccagacggaa cagcaggcag tgctcgacgg ctttcgccac gtattttttc
     8461 acggagctga tggaaacacc gagtttgtgc gcaatctcgc tgtatgtcag accatccagt
     8521 tgcgaaagca gaaacgcttc acgtgttttg ccgtttagcc cgtccagcat gctgtcgagg
     8581 agttgtaggg tctcgagttg gctttcgcgt tcctcaggtg aaggcgctcc cccctccggc
     8641 ataagcgcca gcatctccag atacgctttt tccagcgcgt ttcggcgaaa caggtccacc
     8701 atcacgcgtt tggcgatagt gcagaggaag gagcgaggat cgcggatcgt cgagagcgtt
     8761 tcgctgacca ttacccgcaa aaaagtgtcc tgggcaatgt catctgcatc aaaagcagac
     8821 tggagtttgc gcgtcagcca gcttttcaac cagccgtgat gtgtgccata aagcgactcg
     8881 aacgttaagg aagctgtggt agtggcgcgg tcagacatgc ggagtgcatc aaaagttaat
     8941 tatcacgtag tcatattaat atgagaatgg ttatcattac aattggaaat aaaattgttt
     9001 ccaatagaca tttttaacat gttgtttttc taagtgttat aaggtaggta taaaatggga
     9061 tggagcctct gcttctgaca tgtgtcggtc agaatgactc atgatgtggt ctgctattat
     9121 tgacatcctc actgccctaa aggatgggga tttcggtaat gatcttaccc agcaatagtg
     9181 gacacgcggc taagtgagta aactctcagt cagaggtgac tcacaagaca aaacagtttc
     9241 aaccagtaaa aaaacgtaaa caccattcgc atgaatttcg tagttaagat ctgaagcttg
     9301 cagaatgcat cggtgttact gccggagtcc gtgaacttag tctgcatgaa tcaccacagc
     9361 tttacatctg tcgcagtaaa cagcaaaatc agcaaactta cttaattata tcgagtgcga
     9421 ttacaatcgc tggcgatggt acagttggtg tggcggtctc agtccggaac aatttgaaaa
     9481 tcataatctc acttaaaacg gcatccattt tacaaaagat ggccgtctgt gtatttgtaa
     9541 tgaatacctt ctacatgttt agagtgcata atccacgaca aggcgatatc ctaaaaatta
     9601 gctagcaggc aactttgatg gcatagataa tgtttttagt aaaaaagagt tgactgaata
     9661 taatacagcc tcttcctaca taagaaggaa tgtgctcaca aaaaaaaacc attagtctgc
     9721 ttcctgttct tacaataatc agtattatgt attttattta tatagcaaaa tatgcttgtt
     9781 acacctgtca taattaggca tggtaaaatt attgactgaa ccctggggga gaaagatgca
     9841 cagtgattat tataatatgg tttttggtga aaaattggcc aatattttat atgaagctaa
     9901 ctctcaattt ttttacgaga gaaatgtcat agaagaggca gtcaacgctt tattctgtga
     9961 gcgtgaaata ataaacaaca agaacataat aaagaagctg atgttctttt tgtcagatgt
    10021 taatcacacc aagaaagatg ttgttcagag cgcacttaat attatcattg acattacatc
    10081 aggtgatatt tgagatttta cataaaagat aatctctcaa ggagtcttat ctgaaaacga
    10141 aatatatagt ccctctcgtt ttgtaatatc gattggcttt gttgaatcgg ttacgtaccc
    10201 cggagccggt catattttca ggtaatacac acgctttcag acatttcggc tttcgtcatt
    10261 tttgtatcat gggcatagct tctgctacct gaccatcata atcacgcagc ttcagtgaag
    10321 cagtgaccac ctgtttaacc cggtgtatcg ttatgatctt atccagc
//
LOCUS       DAJTMX010000132        10154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTMX010000132 DAJTMX010000000
VERSION     DAJTMX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.132"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            171..302
                     /locus_tag="OO145_003386"
     CDS             171..302
                     /locus_tag="OO145_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5468385.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            562..1557
                     /gene="pgl"
                     /locus_tag="OO145_003387"
     CDS             562..1557
                     /gene="pgl"
                     /locus_tag="OO145_003387"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5468386.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1598..2551)
                     /locus_tag="OO145_003388"
                     /pseudo
     CDS             complement(1598..2551)
                     /locus_tag="OO145_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2735..3787
                     /locus_tag="OO145_003389"
     CDS             2735..3787
                     /locus_tag="OO145_003389"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5468387.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDIHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3863..5296
                     /locus_tag="OO145_003390"
     CDS             3863..5296
                     /locus_tag="OO145_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5468388.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5479..7659
                     /locus_tag="OO145_003391"
     CDS             5479..7659
                     /locus_tag="OO145_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5468389.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7800..9083)
                     /locus_tag="OO145_003392"
     CDS             complement(7800..9083)
                     /locus_tag="OO145_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5468390.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9218..10099)
                     /locus_tag="OO145_003393"
     CDS             complement(9218..10099)
                     /locus_tag="OO145_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5468391.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2471 a         2367 c         2559 g         2757 t
ORIGIN      
        1 cttcagcaaa ggatttagta atgatcaaaa tcatatctca atgtactgtt tttttcttta
       61 aaaaaacagt acattctatt ttataatgac tgtgtattcc catggaagga gatcacccaa
      121 ttctcccccc cgacttttga cgcagtgtct tgttaacagg gggattgacc atgcttattg
      181 gctatgtacg cgtatcaaca aatgaccaga ataccgatct acaacgtaat gcgtttattt
      241 gtgccggaag tgagttgatt gttgcgcgaa tgggaagttg gccagaagtt gttatggcat
      301 agtgtttacg gtcgttgata caggtaaaca aaagcgggga gagtcatact ccctgaatag
      361 tcgcgaaaat atctgtggca ttgtcaaatt cgtgtcggtg actaatattt tagtaatatg
      421 ccattcgaaa tggagtgcta acttattgat ttaattgatg tgcgatattg tattttatcg
      481 tgttttgttg tctgtttttt atccagtaag tgttgttata tgtatgaaag ataagggatt
      541 tatcattttt gaggtgtgtt catgaagcaa acagtttata tcgccagccc tgagagccag
      601 caaattcacg tctggaacct gaatcatgaa ggcgcactga cgctgacaca ggttgtcgat
      661 gtgccggggc aggtgcagcc gatggtggtc agcccggaca aacgttatct ctatgttggt
      721 gttcgccctg agtttcgcgt cctggcgtat cgtattgccc cggacgatgg cgcactgacc
      781 tttgccgcag agtctgtgct gccgggtagt ccgacgcata tttccaccga tcaccagggg
      841 cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga gcgtaacgcg tctggaagat
      901 ggcctgccag tgggcgtcgt cgatgtagtc gaggggctgg acggttgcca ttccgccaat
      961 atctcaccgg acaaccgtac gctgtgggtt ccggcattaa agcaggatcg catttgcctg
     1021 tttacggtca gcgatgatgg tcatctggtg gcgcaggacc ctgcggaagt gactaccgtt
     1081 gaaggggccg gcccgcgtca tatggtattc catccaaacg aacaatatgc gtattgcgtc
     1141 aatgagttaa acagctcagt ggatgtctgg gaactgaaag atccgcacgg caatatcgaa
     1201 tgtgtccaga cgctggatat gatgccggaa aacttctccg acacccgttg ggcggctgat
     1261 attcatatca ccccggatgg tcgccattta tacgcctgcg accgtaccgc cagtctgatt
     1321 accgttttca gcgtttcgga agatggcagc gtgttgagta aagaaggctt ccagccaacg
     1381 gaaacccagc cgcgcggctt caatgttgat tacagcggca agtatctgat tgccgctggg
     1441 caaaaatctc accacatctc ggtatacgaa attgttggcg agcaggggct actgcatgaa
     1501 aaaggccgct atgcggtcgg gcagggacca atgtgggtgg tggttaacgc acactaaccg
     1561 ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta acttatctcc tgtaacgcat
     1621 gtctctggcg ttcgacgata ttggtccaca aattgtcttt atcgtcagtc cacaaattaa
     1681 tcagcaagga aaaaaagcgt tcagctgccg gagaaagcac ggcatctttg cggcgaataa
     1741 tccccaatgt ccgacgtata acgggttcca ccagtgggat accaataatc gaagaatagg
     1801 gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc cagtcctgcc tccactagtc
     1861 ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc cagcttccag ggcttgtcgg
     1921 ctagctgttg ttctatcaac aatcggttgc cgctggagga acgtacgcca atcattttgt
     1981 aacccactaa ctcctgccat tctacaagct actttttggc caatggatgg tcacgccgac
     2041 aggccagcac gaatggctcg ttgaccagtg gggtaaaatc aatggatgaa tttgtgacgt
     2101 tgttcatgtt tatgccaaag tctgattcgt tacacagcac cgattccatg caattattcg
     2161 tgccttgttc cagaatccgc actttaatat tgggatacag ctcattaaat ttaccgattg
     2221 ccagcggtaa aaaataaaat actgcggtcg gaatacacgc taatgtcacc ataccacgat
     2281 gataagcatt catatcacga atattaaaaa gcgtttcatc gaatttcttt attaattccc
     2341 gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt aaccttgcga gttgtacgct
     2401 caaatagttg aatatgtaaa tcttcctcca tcttttttat tcttcgcgtt aatgcaggtt
     2461 gcgtaatatt gagtaattta gcggcattgt taaatgaact ggactctgcc agtatgacaa
     2521 atgccttcat acttgataat tcatgcttca ttattactcc ggaaaatgga agcgacgatt
     2581 ttggggggct ggtcgttaaa aattttaact gcatttagcc aacttaaatt aatgaaaaaa
     2641 tgttattaat cgttgagcta aagtcattag agatgctttg tccttaatgt aaccatatcg
     2701 caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct gcgtgatgat
     2761 gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg aagatcaaac
     2821 gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg aaattgacgg
     2881 tattggcggc ggtaatccac tgaccagtaa agtcgccatt attagccgtt ccagcgatcc
     2941 gcgtgctgat gtcgattatc tgtttgctca agttatcgtc catgagcaac gtgtcgatac
     3001 cacgcctaac tgcggcaata tgctgtctgg cgtaggggca ttcgccattg aaaatggttt
     3061 gattgcagcg acttcgccag ttacccgtgt acgtatccgc aacgtcaata cgggtacgtt
     3121 catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta gcgccagaat
     3181 tgacggcgta ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg ccgctggaac
     3241 gaaaactgga aaagttttcc cgactgataa tcagattgat tattttgacg atgtcccggt
     3301 gacctgtatc gatatggcga tgccagtcgt cattattccg gctgaatatc tgggaaaaac
     3361 aggttatgaa ttaccggcgg aactggatgc cgacaaagca ttattagccc gcattgaatc
     3421 tatccggcta caagcgggta aagcaatggg cttaggtgat gtcagtaata tggttatccc
     3481 taaacctgtg cttatttctc cagcgcagaa aggcggggca attaatgtgc gttattttat
     3541 gccgcattct tgccatcgcg cgctggcgat aaccggtgct attgctattt ccagtagttg
     3601 tgcattggaa ggcaccgtca cccgacaaat cgtcccttct gtaggatatg gcaatatcaa
     3661 tattgaacac cccagtggtg cgctcgacat tcatttaagt aatgaaggtc aggatgccac
     3721 gacgttacgc gcatctgtta ttcggacaac cagaaaaata ttttccggtg aagtttatct
     3781 tccctgaaaa aattcgttgt caggataagg acaatcaata aaggacttct gtatgagtca
     3841 tacagaaaga acaggatttt aaatgaataa gaaatcgtta tggaagctaa ttctgatatt
     3901 agcgatccca tgtattattg gttttatgcc agctccggca ggattaagcg aactggcgtg
     3961 ggtgcttttt ggtatttacc tggcggccat tgtgggtctg gttatcaagc ctttcccgga
     4021 acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg gtggtcggta acttatccga
     4081 cggtgcgttt aagaccaccg ccgtattaag cggttactct tcaggtacta cctggctggt
     4141 gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta aacttattgc
     4201 ctatctgctg attggtaaaa tcggtaacac catgctgggt ctgggttacg ttacggtatt
     4261 cctcgatctg gtactggctc cggcaacacc gtctaacacc gcgcgtgcgg gcggcattgt
     4321 gttaccgatc atcaacagcg tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg
     4381 tcgtgtcgga cattacctga tgatgtccat ttacatggtc accaaaacca ccagctatat
     4441 gttctttacc gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg acattctgca
     4501 cctgcaaatt agctggggtg gatgggcgct ggccgccgga ttgccgggca tcattatgct
     4561 gctggtcacc ccgctggtaa tttacaccat gtatccacca gaaattaaaa aggtggataa
     4621 caaaaccatc gctaaagcgg gccttgccga actgggaccg atgaaaatcc gcgaaaaaat
     4681 gctgctcggt gtctttgtgc tggcgctact gggctggatt ttcagtaagt ctctgggggt
     4741 tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg ctgctgctgg gtatcgttac
     4801 ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt acggcggtat
     4861 tatcggctta agctccttat tatcgaaagt taaatttttc gaatggttag ctgaagtctt
     4921 taaaaataac ctggcatttg atggtcacgg taacgttgct ttcttcgtta ttattttcct
     4981 cagcatcatc gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc
     5041 tgtatttgcc atgctggcga acgtctccgg cgcaccgtta atgttaaccg cgctggcact
     5101 gttgttctcc aactcctatg gcggcatggt tactcactat ggcggcgcgg caggtccggt
     5161 catctttggc gtgggttaca acgatattaa atcctggtgg ttggtcggtg cggtactgac
     5221 gatattaacc ttcttggtgc atatcaccct cggcgtgtgg tggtggaata tgctgatcgg
     5281 ctggaacatg ctgtaaatat actcgtcata cttcaagttg catgtgctgc gtctgcgttc
     5341 gctcacccca gtcacttact tatgtaagct cctggggatt cactcacttg tcgccttcct
     5401 gcaactcgaa ttatttagag tatatccatt tattatcttt ctgcgcactt cacggtgcgc
     5461 agatatctgg agcatttgat gatcaagtta tctgaaaaag gcgtgtttct cgccagtaat
     5521 aacgaaataa ttgccgaaga acatttcacc ggcgaaatta aaaaagaaga agcaaaaaaa
     5581 ggcactattg cctggtctat tctctcttct cataatacgt ccggaaatat ggataaactt
     5641 aaaattaagt ttgattcatt agcctctcac gatattacct ttgttggtat tgtacagacc
     5701 gctaaagcgt ccggaatgga acgtttcccg ctgccgtatg tgctgaccaa ctgccataac
     5761 tcactctgcg ccgtcggcgg caccattaac ggtgatgacc atgtttttgg tttatcggca
     5821 gctcagcgtt atggcggtat ttttgtgcct ccgcatattg cggtcatcca tcaatatatg
     5881 cgtgagatga tggctggcgg gggcaaaatg atcctcgggt cagacagcca cacccgttac
     5941 ggtgcattag ggacaatggc agtcggtgag ggtggcggtg agttggtaaa acagctgctt
     6001 aatgacacct gggatatcga ctatccgggc gtggttgcgg tgcatctgac cggaaaacct
     6061 gcgccgtatg tggggccaca ggatgtggcg ctggctatca ttggcgcggt gttcaaaaac
     6121 ggttacgtca aaaacaaagt catggagttc gttggaccgg gcgttagcgc gctctctacc
     6181 gatttccgta acagcgttga cgtgatgacc acggaaacca cctgtttaag ctccgtctgg
     6241 caaactgatg aagaagtgca taactggctg gcgctgcacg gtcgcggtca ggattactgc
     6301 cagcttaacc ctcaaccgat ggcgtactac gatggctgca tcagcgttga tttaagcgcc
     6361 atcaaaccaa tgattgcgct gccgttccac ccgagcaacg tgtatgaaat cgacacactg
     6421 aaccagaacc tgaccgacat tctgcgtgag attgaaattg agtccgaacg cgtggcgcac
     6481 ggtaaagcca aactctcgct gctggataaa gtggaaaatg gtcgcctgaa agtgcagcag
     6541 gggattatcg cgggctgttc tggcggtaac tacgaaaacg tcatcgcggc ggcgaatgca
     6601 ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg cggtttaccc gtcatcacag
     6661 ccggtgttta tggatctcgc caaaaaaggt gtggtagcag atttgattgg cgcaggcgca
     6721 atcatcagaa ccgcattctg tggcccatgc tttggcgcgg gcgatacgcc aatcaacaac
     6781 ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc gcgaaggctc taagccagcc
     6841 aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt ctatcgctgc gactgcggca
     6901 aacggtggct atttaacctc tgccagcgaa cttgattgct gggacaacgt gccggagtac
     6961 gccttcgatg taacgccgta taaaaaccgt gtttatcagg gctttgtgaa aggggcaact
     7021 cagcaaccgc tgatttacgg accgaacatt aaagactggc cggaattggg tgcgctgact
     7081 gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg aagtcaccac caccgacgaa
     7141 ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc cgattggtct ggcggggaat
     7201 gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga ttgctggtca ggagcatatt
     7261 gatccgctgc aaactgaaat tggcagcatg gtctatgcgg tgaaaccagg cgatggttct
     7321 gcgcgtgaac aggcggcgag ctgccagcgt gtgattggcg gtctggcgaa tattgccgag
     7381 gagtacgcga ctaaacgcta ccgttctaac gtcatcaact gggggatgtt accgctgcag
     7441 atggcggaag tgccaacctt tgaagtgggg gattacattt acatccctgg cattaaagcg
     7501 gcgctggata atccgggtac gacgtttaaa ggttatgtga tccatgaaga tgcgccggta
     7561 acggaaatta cgctttacat ggaaagcctg acggctgaag agcgcgagat tatcaaggcg
     7621 ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa aagcgccatg tgaatgtagg
     7681 tcgcattcgg cacttattgt cggatgcgat gctggcgcat cttatccgac ctacaaatcg
     7741 catcgaaccg taggccggat aaggcgttta cgccgcatcc ggcaaatagt taatagctct
     7801 tacttcttct cctctgcaac cactttgctg cccacgccgc ggttattgta ttcccacatg
     7861 cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt cgttatcatc tacgctgccg
     7921 gtattacccg caaacggacg attagagatc accgcatcgg cccacggttt agccgtgtta
     7981 aaaccttcgt tgatggcgct atcacggatc accacctgac cgttggtatt agcatcaaca
     8041 tccagcgagc ggcccagttg cgccacgcca tcaccggaag cattgaaacg gctgtttacg
     8101 gcgaggaaac cgtaatagat gttagacagc gtagccggtg caaacacata tgcttcttgc
     8161 tgggtacgtg agttcaccac gcggaattcg gtgttatcga acaccactgc gccgcgacca
     8221 gaaacgatat ccacatcccc ttcaatatag ctgttggtca ccagcgtacg cggctgacgg
     8281 ttggtttcca gacggttctg cacaccgctg ttggtgacaa agaaggtgtt ctgacgaccg
     8341 agaatgttaa cgttgttaat ctgtacttgg tcaccgtcag tacgcagtgc taccgccgga
     8401 tggttacctg catctacgct atcgcccagc gtgttttcga tggtcagatt ttgcagttgc
     8461 agtccattgt tttgtgacca gaagaccgca gaacacagaa caccgatact gtcgctgcgt
     8521 ttgctctggc agctatcgta catataccac gctggtttac ctggcatata tttgccgcgc
     8581 gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc catcaaggga aagcccaatc
     8641 ttcacatcaa tcggtttttc acctgtaccg tacagagtga ttccacctgg agcagcaggg
     8701 acatacaccg ttccctgata ctcaccaggc atcacggcaa tatactggcg cttgttggtg
     8761 cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg tatgcgttac accttgagta
     8821 cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg ttatcgggga cggattccac
     8881 gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgcg catcgaaatt cagcgcttct
     8941 tttgccgaca gaatcgggcg agaagaggta ccaggcgcag tttgatcaga aggacgttga
     9001 tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa aagccaatgc cagcgccaga
     9061 cgggaaactg aaaatgtgtt cacaggttgc tccgggctat gaaatagaaa aatgaatccg
     9121 ttgaagcctg cttttttata ctaagttggc attataaaaa agcattgctt atcaatttgt
     9181 tgcaacgaac aggtcattat cagtcaaaat aaaatcatta tttgatttca attttgtccc
     9241 actccctgcc tctgtcatca cgatactgtg atgccatggt gtccgacttg tgcccgagaa
     9301 gatgttgagc aaacttatcg cttatctgct tctcatagag tcttgcagac aaactgcgca
     9361 actcgtgaaa ggtaggtgga tccccttcga aggaaagacc tgatgctttt cgtgcgcgca
     9421 taaaatacct tgatactgtg ccggatgaaa gcggttcacg acgagtagat gcaattatgg
     9481 tttctccgcc aagaatctct ttgcatttat caagtgtttc cttcattgat atcccgagag
     9541 catcaacatg caatgttgta gggatggcaa tttttacgcc tgttttgctt tgctcgacat
     9601 aaagatatcc atctacgata tcagaccact tcatttcgca taaatcacca actcgttgcc
     9661 cggtaacaac agccagttcc attgcaagtc tgagccaaca tggtgatgat tctgctgctt
     9721 gataaatttt caggtattcg tcagccgtaa gtcttgatct ccttacctct gattttgctg
     9781 cgcgagtggc agcgaccggg tttgttgtta tatggccttc agctattgcc tctcggaatg
     9841 catcgctcag tgttgatctg attaacttgg ctgatgccgc cttgccctcg tctatgtatc
     9901 cattgagcat tgccgcaatt tcttttgtgg tgatgtcttc aagtggagca tcaggcagcc
     9961 ccctccttat tgctttaatt ttgctcatgt aatttatgag tgtcttctgc ttgattcctc
    10021 tgctggcgag gattttttcg tagcgatcaa gccatgaatg taacgtaaca gaattatcac
    10081 tgttgattct cgctgtcaga ggcttgtgtt tgtgtcctga aaataactca atgttggcct
    10141 gtatagcttc agtg
//
LOCUS       DAJTMX010000133        10015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTMX010000133 DAJTMX010000000
VERSION     DAJTMX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.133"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1636)
                     /gene="groL"
                     /locus_tag="OO145_003394"
     CDS             complement(<1..1636)
                     /gene="groL"
                     /locus_tag="OO145_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5468392.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMG"
     gene            complement(1680..1973)
                     /locus_tag="OO145_003395"
     CDS             complement(1680..1973)
                     /locus_tag="OO145_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5468393.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2249..3505
                     /gene="yjeH"
                     /locus_tag="OO145_003396"
     CDS             2249..3505
                     /gene="yjeH"
                     /locus_tag="OO145_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5468394.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(3521..3997)
                     /gene="fxsA"
                     /locus_tag="OO145_003397"
     CDS             complement(3521..3997)
                     /gene="fxsA"
                     /locus_tag="OO145_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5468395.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            4334..5770
                     /gene="aspA"
                     /locus_tag="OO145_003398"
     CDS             4334..5770
                     /gene="aspA"
                     /locus_tag="OO145_003398"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5468396.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            5888..7189
                     /gene="dcuA"
                     /locus_tag="OO145_003399"
     CDS             5888..7189
                     /gene="dcuA"
                     /locus_tag="OO145_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5468397.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7305..7643
                     /gene="cutA"
                     /locus_tag="OO145_003400"
     CDS             7305..7643
                     /gene="cutA"
                     /locus_tag="OO145_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5468398.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            7619..9316
                     /gene="dsbD"
                     /locus_tag="OO145_003401"
     CDS             7619..9316
                     /gene="dsbD"
                     /locus_tag="OO145_003401"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5468399.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9353..9928
                     /locus_tag="OO145_003402"
     CDS             9353..9928
                     /locus_tag="OO145_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5468400.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2341 a         2607 c         2500 g         2567 t
ORIGIN      
        1 cgcccatgcc acccatgccg cccataccgc cagcagcgcc taagtcagct gcatcgtttt
       61 tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag gccagccaca gaagctgcgt
      121 actgcagagc agaacgggtt actttggttg ggtccaggat acccatgtcg atcatgttgc
      181 cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta acggtgttag
      241 caacaacaga cggttcttcg ccgcagttca ggacgatctg acgcagcgga gcttccattg
      301 cacgcagtgc aactttgata cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca
      361 gtttagacgc tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg
      421 cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt tttctctttc atttcaactt
      481 cggtagcagc acctactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct
      541 gcagtttttc acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa
      601 cacggccctg gattgcagct tcttcaccca cgccatcgat gatagtggtg gtgtctttgt
      661 tgatcacaac acgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac
      721 cgatctcttc agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt
      781 tacgacgatc gccgaagccc ggagctttaa ccgcagcaac tttcacgatg ccacgcatgg
      841 tgttaacaac cagagttgct agcgcttcgc cttcaacatc ttcagcgatg atcagcagcg
      901 gtttgcctgc tttggcaaca gcttccagaa ccggcagcat ttcgcggatg ttggagattt
      961 tcttgtcagc cagcaggatg aacgggcttt ccagttctac tgcgccagtt tccggcttgt
     1021 tgatgaagta aggagacagg tagccacggt cgaactgcat accttcaacc acgtccagtt
     1081 cgtcctgcag accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca
     1141 tcgcttcagc gatcagttta cctacggttt cgtcggagtt agcggagatg gtaccaacct
     1201 gagcaatcgc tttagaatca gagcacggta cggacagcgc tttcagttct tcaactgcag
     1261 cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag
     1321 ctttcagacc ttcagtgatg atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc
     1381 ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat ctgcgcaccc atattttcga
     1441 acttgtcttc cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg
     1501 caccgaaaga tttatccaga actacgttac ggccttttgg accgagggta actttcactg
     1561 catctgccag tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta
     1621 cgtctttagc tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgcggat
     1681 tacgcttcaa caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg
     1741 atcttctcgg atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcacg
     1801 tccagcggct tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg
     1861 ccgcgggtgg atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt
     1921 tcaacttctt tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac
     1981 tctcctttga gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg
     2041 ctggtgacca gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa
     2101 ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc
     2161 ttcatcgccg tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca
     2221 aaatttgggc gcaatcggat atcaacccat gagtggactc aaacaagaac tggggctggc
     2281 ccagggcatt ggcctgctat cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc
     2341 gttagctgca ctggtagcag gcaataacag cctgtgggcg tggcccgttt tgattatctt
     2401 agtgttcccg attgcgattg tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg
     2461 cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca ccggctggct
     2521 gtttttatcg gtcattcccg tgggtttgcc tgccgcacta caaattgccg ccggattcgg
     2581 ccaagcaatg tttggctggc atagctggca actgttgttg gcagaactcg gtacgctggc
     2641 gttggtgtgg tatatcggta ctctcggtgc cagttccagt gctaatctac aaaccgttat
     2701 tgccggactt atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc
     2761 gagtatcccc ttccccgcgc caggaaatat cgaacttacc gggttatttg ctgcgttatc
     2821 agtgatgttc tggtgttttg tcggtctgga agcatttgcc catcttgcct cggaatttaa
     2881 aaatccagag cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg caggattagt
     2941 ctactggggc tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc
     3001 ggcagcatcg cttccaaaaa ttgtagtaca gttgttcggt gtaggagcgt tatggattgc
     3061 ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac atttatatac agagcttcgc
     3121 ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac tacctggcac gcctctcttc
     3181 tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc tgctgcgtgg tgagcacgtt
     3241 ggtaattcat gccttagaga tcaatctgga cgctcttatt atttatgcca atggcatctt
     3301 tattatgatt tatctgttat gcatgctggc aggctgtaaa ttattgcaag gtcgttatcg
     3361 actactggcg gtggttggcg ggctgttatg cgttctgtta ctggcaatgg tcggctggaa
     3421 aagtctctat gcgctgatca tgctggtggg gttatggctg tttctgccaa aacgaaaaac
     3481 gccggaaaat ggcataacca cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt
     3541 tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata ctcaccatca aaagtattac
     3601 cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca
     3661 tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt
     3721 cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat aatcagcgaa acacttttaa
     3781 tcatctccgc cgcagggttt tcacccgccg ccattttttg ctgcatcagc acgaaattct
     3841 taaagccctg gttacgtacc agtgacatac cgataactga cgtgaatata accagcacga
     3901 gggtcagcaa tacccccaat acatgggcaa cctgaataaa gattgaaatc tcaatataga
     3961 cataaaggaa aatggcaata aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc
     4021 ggttaaaccc ggctacctga atgggttgcg aatcgccttc agcttatatt gtggtcatta
     4081 gcaaaatttc aagatgtttg cgcaactatt tttggtagta atcccaaagc ggtgatctat
     4141 ttcacaaatt aataattaag gggtaaaaac cgacacttaa agtgatccag attacggtag
     4201 aaatcctcaa gcagcatatg atctcgggta ttcggtcgat gcaggggata atcgtcggtc
     4261 gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag caaatcattg gcagcttgaa
     4321 aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa gatctgttgg gtaccaggga
     4381 agttccagct gatgcctact atggtgttca cactctgaga gcgattgaaa acttctatat
     4441 cagcaacaac aaaatcagtg atattcctga atttgttcgc ggtatggtaa tggttaaaaa
     4501 agccgcagct atggcaaaca aagagctgca aaccattcct aaaagtgtag cgaatgccat
     4561 cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt tcccggtaga
     4621 cgtctaccag ggcggcgcag gtacttccgt aaacatgaac accaacgaag tgctggccaa
     4681 tatcggtctg gaactgatgg gtcaccagaa aggtgaatat cagtacctga acccgaacga
     4741 ccatgttaac aaatgtcagt ccactaacga cgcctacccg accggtttcc gtatcgcagt
     4801 ttactcttcc ctgattaagc tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg
     4861 taaagctgtc gaattccagg acatcctgaa aatgggtcgt acccagctgc aggacgcagt
     4921 accgatgacc ctcggtcagg aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa
     4981 aaacatccaa cgtaccgctg aactgctgct ggaagttaac cttggcgcaa cagcaatcgg
     5041 tactggtctg aacacgccga aagagtactc tccgctggca gtgaaaaaac tggctgaagt
     5101 cactggcttc ccatgcgtac cggctgaaga cctgatcgaa gcgacctctg actgcggcgc
     5161 ttatgttatg gttcacggcg cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa
     5221 cgacctgcgc ttgctctctt ctggtccacg tgccggcctg aacgagatca acctgccgga
     5281 actgcaggcg ggctcttcca tcatgccagc taaagtaaac ccggttgttc cggaagtggt
     5341 taaccaggta tgcttcaaag tcatcggtaa cgacaccact gttaccatgg cagcagaagc
     5401 aggtcagctg cagttgaacg ttatggaacc ggtcattggc caggccatgt tcgaatccgt
     5461 tcacattctg accaacgctt gctacaacct gctggaaaaa tgcattaacg gcatcactgc
     5521 taacaaagaa gtgtgcgaag gttacgttta caactctatc ggtatcgtta cttacctgaa
     5581 cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa
     5641 gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact gaagcggaac ttgacgatat
     5701 tttctccgta cagaatctga tgcacccggc ttacaaagca aaacgctata ctgatgaaag
     5761 cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca cgtcagatga cgtgcctttt
     5821 ttcttgtgag cagtaactta aaaataacaa tctaatatca acttgttaaa aaacaaggaa
     5881 ggctaatatg ctagttgtag agctcatcat agttttgctg gcgatcttct tgggcgccag
     5941 attgggggga ataggtattg gttttgcagg cggattgggg gtgctggttc ttgccgctat
     6001 tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc attatcatgg cggttatcgc
     6061 cgctatttct gccatgcagg ttgctggcgg tctggactat ctggttcatc agacagaaaa
     6121 gctgctgcgc cgtaacccga aatacatcac gatcctcgca ccgatcgtga cctatttcct
     6181 gactatcttt gctggtactg gcaacatctc tctggcgaca ctgccagtta tcgctgaagt
     6241 tgcgaaggaa caaggcgtta aaccttgccg tccgctctct actgcagtgg tatccgcgca
     6301 gattgcgatc accgcatcgc caatctcagc ggcagtggtt tacatgtctt ccgtgatgga
     6361 aggtcatggc atcagctacc tccatctgct ctccgtggtc atcccgtcca ccctgctggc
     6421 ggttctggtg atgtccttcc tggtcactat gctgttcaac tccaaactct ctgacgatcc
     6481 gatttatcgc aagcgtctgg aagagggctt ggttgaactg cgcggtgaaa agcagattga
     6541 aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt
     6601 tatctatgca atcatcaaca gcccaagcat gggtctggtt gaaaaaccgc tgatgaacac
     6661 caccaacgca atcctgatca tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa
     6721 agtcgatacc gacaacatcc tcaactccag caccttcaaa gcaggtatga gcgcctgtat
     6781 ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt tccaacaaca tcgactggat
     6841 caaagatacc gctggtgaag tgattcaggg tcatccgtgg ctgctggccg tcatcttctt
     6901 ctttgcttct gctctgctgt actctcaggc tgcaaccgca aaagcactga tgccgatggc
     6961 tctggcactg aacgtttcac cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct
     7021 gttcattctg ccgacctacc cgacgctggt tgctgcggta cagatggatg acacgggtac
     7081 tacccgtatc ggtaaattcg tcttcaacca tccgttcttc atcccgggta ctctgggtgt
     7141 tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc atgctgtaat gatcaatcgc
     7201 ggggcgttca cgccccgctt tctttcccgc cgactaacat ccttcccccg tccgttgtat
     7261 agtgacctct ctcttgcggt tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa
     7321 gttcgaatac cacgtctgtc gtggtgctat gtaccgcacc ggatgaagcg acagcccagg
     7381 atttagccgc caaagtgctg gcggaaaaac tggcggcctg cgcgaccttg atccccggcg
     7441 ctacctctct ctattactgg gaaggtaagc tggagcaaga atacgaagtg cagatgattt
     7501 taaaaactac cgtatctcac cagcaggcac tgctggaatg cctgaagtct catcatccat
     7561 atcaaacccc ggaacttctg gttttacctg ttacacacgg agacacagat tacctctcat
     7621 ggctcaacgc atctttacgc tgattctgct actttgcagc acttccgttt ttgccggatt
     7681 attcgacgcg ccgggacgtt cacaatttgt ccccgcggat caagcctttg cttttgattt
     7741 tcagcaaaac caacatgacc ttaatctgac ctggcagatc aaagacggtt actacctcta
     7801 ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt gccgacgagc agctgccgca
     7861 aggcgtctgg catgaagatg agttttacgg caaaagcgag atttaccgcg atcggctgac
     7921 gcttcccgta accatcaacc aggcgagtgc gggagcgacg ttaactgtca cctaccaggg
     7981 ctgtgctgat gccggtttct gttatccgcc agaaaccaaa accgttccgt taagcgaagt
     8041 ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg cagcaagagc agcacaccgc
     8101 gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt attggtatcg cctttacgcc
     8161 atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg ctgggtggta aacagcggct
     8221 ctccactgcc agagcattgt tgctgacctt tatttatgtg caggggatgg cactgactta
     8281 cacggcgctg ggtctggtag ttgccgccgc agggttacag ttccaggcgg cgctacagca
     8341 cccatacgtg ctcattggcc tcgccatcgt ctttaccttg ctggcgatgt caatgtttgg
     8401 cttgtttact ctacaactcc cctcttcgct gcaaacacgt ctcacgttga tgagcaatcg
     8461 ccaacagggc ggctcaccta gcggtgtgtt tgttatgggg gcgattgccg gactgatctg
     8521 ttcaccatgc accaccgcac cgcttagcgc gattctgctg tatatcgccc aaagcgggaa
     8581 catgtggccg ggcggcggca cgctttatct ctatgcgttg ggcatgggcc tgccgctgat
     8641 gctaattacc gtctttggta accgcttgct gccgaaaagc ggcccgtgga tggaacaagt
     8701 caaaaccgcg tttggttttg tgatcctcgc actgccggtc ttcctgctgg agcgagtgat
     8761 tggtgatgta tggggattac gcttgtggtc ggcgctgggt gtcgcattct ttggctgggc
     8821 ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct
     8881 ggcagcggca ttggttagcg tgcgcccact tcaggattgg gcatttggtg cgacgcatac
     8941 cgcgcaaact caaacgcatc tcaactttac acaaatcaaa acggtagatg agttaaatca
     9001 ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat ctttatgccg actggtgcgt
     9061 cgcctgtaaa gagtttgaga aatacacctt cagcgacccg caggtgcaaa aagcgttagc
     9121 agacacggtc ttacttcagg ccaacgtcac ggccaacgac gcacaagatg tggcgctgtt
     9181 aaagcatctt aatgtccttg gcctaccgac aattctcttt tttgacggac aaggccagga
     9241 gcatccacaa gcacgcgtca cgggctttat ggatgctgaa accttcagcg cacatttgcg
     9301 cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg gaggaaaata gcgtgcaacg
     9361 tgaagatgta ctgggagaag ccctgaaatt attagaatta caagggattg ccaacaccac
     9421 gctggagatg gttgctgaac gtgtggatta tccactggac gagctacgcc gcttctggcc
     9481 agacaaagag gcaatcctct acgatgcgct gcgttatctt agccaacaga tagatgtctg
     9541 gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa caaaagctgc tggcacgtta
     9601 tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg ggctgtctat ttatcgctgc
     9661 ctgtacgttt tatcccgatc ctggccaccc tattcatcaa ctggccgatc agcaaaaaag
     9721 cgctgcctac gatttcaccc acgaactgtt aaccacgctg gaagttgacg atccggcgat
     9781 ggtagcaaag cagatggaac tggtgctgga aggctgttta agccgaatgc tggtgaatcg
     9841 tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa gatatcctgc gcttcgcccg
     9901 ctgccgccag ggtggtgcat tgacctgaca gaaacacaga aaagaagcga tttgccgcaa
     9961 tcttaagcag ttgaatcgct tttactgaaa ttaggttgac gagatgtgca gatta
//
LOCUS       DAJTMX010000134         9937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTMX010000134 DAJTMX010000000
VERSION     DAJTMX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.134"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="OO145_003403"
     CDS             complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="OO145_003403"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5468401.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="OO145_003404"
     CDS             complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="OO145_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5468402.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="OO145_003405"
     CDS             complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="OO145_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5468403.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4163..4888)
                     /gene="phoU"
                     /locus_tag="OO145_003406"
     CDS             complement(4163..4888)
                     /gene="phoU"
                     /locus_tag="OO145_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5468404.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4903..5676)
                     /gene="pstB"
                     /locus_tag="OO145_003407"
     CDS             complement(4903..5676)
                     /gene="pstB"
                     /locus_tag="OO145_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5468405.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5768..6658)
                     /gene="pstA"
                     /locus_tag="OO145_003408"
     CDS             complement(5768..6658)
                     /gene="pstA"
                     /locus_tag="OO145_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5468406.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6658..7617)
                     /gene="pstC"
                     /locus_tag="OO145_003409"
     CDS             complement(6658..7617)
                     /gene="pstC"
                     /locus_tag="OO145_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5468407.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7704..8744)
                     /gene="pstS"
                     /locus_tag="OO145_003410"
     CDS             complement(7704..8744)
                     /gene="pstS"
                     /locus_tag="OO145_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5468408.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8992..>9937)
                     /locus_tag="OO145_003411"
     CDS             complement(8992..>9937)
                     /locus_tag="OO145_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5468409.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2564 a         2585 c         2294 g         2494 t
ORIGIN      
        1 aatccaaccg gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc
       61 atattcagta tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca
      121 gtcatgtaat aactaaaaga gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt
      181 tcagtcatct caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatat
      241 tggccacgcg cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctgc
      301 aacatatcct gtggctggca ggtgaggggg taaaccagcc caccgagaag catattgccg
      361 attttcgctt cggggatcag gctatgatag gctttaactg cccgcgcact ggcaaccagt
      421 tgatgatgga tcgcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc
      481 gtgaatggcg cgtgtaacga catattgatt tcattaaacg tcagccataa cgctacttta
      541 tgttggtagc gggtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgcccga
      601 ttagcccaac cgccgtagtt tttcaccagc ccatatagca tttcgtaatg ggataacgtt
      661 accagcggct tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac
      721 cccgcttcat tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa
      781 atacgcagac aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtaa
      841 taaaaatcga tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt
      901 cccattacgc catgaggctg taaatctgag gtcgtgatcc ctttgccatc ttccagccag
      961 gcaccttcca cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct
     1021 ttcataatta actcctttta tcgttagcga atgatggata acagcggttc acctgcgctt
     1081 atctgcgccg tgccgtgggg taatacgtct gtaaaatcat cgctattact gattaatacc
     1141 ggcgtcgtca gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga
     1201 tcgcctgtat tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt
     1261 tttacggtgt cgataccgac atgaatcagg atctccacac catcatctga ctcaatgcca
     1321 atggcgtgta atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca
     1381 ccaaccgagg gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca
     1441 gcgacgtgaa tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca
     1501 ggtggtgtaa taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta
     1561 ctggtgatga aatgaagcat caacgtacca acaaatgcgc aacctatggc aatgacaccg
     1621 ccaataacgc tggcccagac ggtgaaatca atccccgttg acgggatggt ctgcatgaag
     1681 gtgaaaatac ttggcaaacc aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg
     1741 gcccccaatg ctccactgat acaggcgata acaaaggggt acttacgcgg caggttgacg
     1801 ccatataccg ctggttcggt gataccaaac agacccgtca acgccgctga tcccgccacc
     1861 acttttttct gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc
     1921 ataatggcgg gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg
     1981 atacacagcg gcaccaggcc cctgtgcagt ccgaacatga cgaagatttg ccagaagccg
     2041 cccattaccg cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcggca
     2101 atcatttcgc ttatccaggt tgatagctgc cccaccagca gaaaggtgac gggtgtgata
     2161 accatcagac atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta
     2221 agtcggcgtt ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac
     2281 gagtaattca acaatgtgac cggaataccc aggaaatcca gccccggcgc atccgctttt
     2341 tgcccgttct cgaaggcagt cagaattaat ggatgcacta acgctccacc aatcaccatg
     2401 gcagtaaatg gattaccgcc gaagcgtttg cccgcggtgt atcccaggat tatcgggaag
     2461 aacccaaaca aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc
     2521 gtccactgaa aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc
     2581 agaccgatca gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta
     2641 cctttctcat cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgtta
     2701 accgccagga agacatcggc cacatggtta cctatgacca cctgaaactg accaccgctt
     2761 tccaccacca taataatacc gggggtcttt ttcagtacct cagcttgcgc tttgctttca
     2821 tcctttaact taaaacgtaa tcgcgttgcg caatgcatca gactcacaat gttatctgcg
     2881 cccccgactc ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt
     2941 tgcggcaaaa ctccaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt
     3001 tttcaggttt tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg
     3061 ttctttgcgc acgcgctcta tatttatggc taaaaacata atctctgcgg gtgaaatttt
     3121 acgttgatac tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg
     3181 gtaattttgt tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc
     3241 aagaatacgc caggataaaa acttcagatg tgtaaccagt cgctgataac tcaagctttc
     3301 ttcctggtaa ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaactg
     3361 cgtgacacct gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc
     3421 aataaagccc acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa
     3481 cgcttcttcc cctagctgga actctttcgg gtaaagccgc tggatatccc acagcaacac
     3541 gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagtgaga tataaatact
     3601 gtcctgtaat tttcccaagc gctcctgcgc taaagagata agacgatcac aggttgccat
     3661 cacctcaaga ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct
     3721 caaggcatac tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa
     3781 gccaattccg cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac
     3841 attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa
     3901 aaaaaaaccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
     3961 acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga aattaattta
     4021 attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta
     4081 ttaactttgt gtaattttag gaatttataa agttatatat aacaaatccc aataattaag
     4141 ttattgggat ttgtctggtg aattatttgt cgctatcttt tcccgccagc agtttatcca
     4201 gctcatcacc gcctacatga cggaaatcct gacccttcac gtagtagaag ataaactcac
     4261 aaatattctg gcagcggtcg ccgatacgtt cgattgaacg cgcacagaac agcgcggtaa
     4321 gtacgctcgg aatagtgcgc gaatcttcca tcatgtaggt catcagttga cgaacaatac
     4381 cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
     4441 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
     4501 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
     4561 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
     4621 taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt
     4681 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca
     4741 gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc
     4801 cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa
     4861 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
     4921 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
     4981 ctcaatcaat tcgcccaggt acataaatgc cgtgtggtcg gaacaacgcg cagcctgctg
     5041 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc
     5101 aatacgcccg gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc
     5161 cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga
     5221 gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
     5281 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa
     5341 ggcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac
     5401 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag
     5461 aatttcacct tccgcacgct gctccgggta cagttcaaac attttgttga aggtacgcag
     5521 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gtaacctggt ttttggcgat
     5581 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
     5641 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtacct
     5701 gatgcttttc gctaatcagg cttaccgtag ggccgggtaa ggcgttcacg ccgcatccgg
     5761 aaaagtgtta accgtgttta ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca
     5821 ggacgcacag ggtaatgatc aataccccgg cccaggccag ttgttgccat tccgcaaacg
     5881 ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc gatcggctgc atcatgtccg
     5941 tgctccagaa ctggttggag agcgcggtaa acagcagcgg cgcggtttca ccggcaatac
     6001 gggcaatcgc cagcaggata ccggtcataa tcccggacac cgacgctttc agcgtaatcg
     6061 cggagatcat cttccacttc ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg
     6121 gcaccagttt cagcatgttc tcagtggtgc ggataacgat cggcacctgc aacagcgcca
     6181 gcgcaattac gcccgcccag ccggagaagt gctccatctg tgccaccacg atggtgtaga
     6241 caaacagacc aaccacaatc gacggcgcgg agagcagaat gtcgttaatg aagcgaatca
     6301 cttctgccag ccaggattta cgaccatatt ccgccagata aatccccgcc ataatgccca
     6361 gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc gctacccgcc agggcgttcg
     6421 ccagaccacc accttccgta ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca
     6481 taccgtcgat accgcgagtg atggtggaca ttaaaatcca gatcagccag aacaggccga
     6541 aggccatcgt cgccatcgag agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct
     6601 gcattttgcg gcgagattca gccagcgccg cagtggtttg catttcaacc atagccatta
     6661 gcgtgccccc tcattcttag ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat
     6721 gaaggtaatc acaaacagga tcaggcccag ttccatcagt gcggcaacgt gcagaccgga
     6781 ttccgcttcc gcaaattcgt tcgccagcgc agaggtgata ctattgcccg gcatgtacag
     6841 cgaggcgctg tcgagctggt aggtgttacc gatgataaag gtcaccgcca tggtttcacc
     6901 gagcgcgcgg cccagcccca gcatgatgcc gccgataaca ccatttttgg tgaacggaag
     6961 aacgatacgc cagataactt cccaggtggt gcagccaata ccgtaggccg actctttcat
     7021 catcaccggg gtttgttcga acacatcacg cattaccgcc gcaatgtacg gaataatcat
     7081 gatggcgagg atcacgcctg ccgcgaggat accgatacca aatgcggggc cagagaacag
     7141 cgcgccaaca atcgggatat tcgacatgat attgccgacc ggctcctgaa agtaaacggc
     7201 gaacagcggc gcaaagataa acaggcccca catgccgtaa acgatacttg gaatggctgc
     7261 cagcagctca atggcgatac ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag
     7321 gaacagggcg ataccgaaac tcaccgggac ggcgatcagc agcgcgataa acgaagtcac
     7381 caacgtaccg tagatcggca ccagcgcccc gtagatatcg ttcggtgcat cccactcttt
     7441 ggtccatagg aaagccagac caaatttctg aatgctcggc caggaggaga tgatcagaga
     7501 gacaataatg ccacccaaca tcaatagcac aatcagcgcc gccagtttta ccagcacgct
     7561 gaaaattatg tcgccctttt tacccggtgg gttaaaagcg ggcttggttg cagccataag
     7621 ttactcttca gttaaacgcg tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac
     7681 accgtacccg gcctggagtt ttattagtac agcggcttac cgctactgtc tttaatattg
     7741 gtcttccacg cagcgcgaac ctgttcaact acactatccg gcaggctggc gtaatccagg
     7801 tcgttcgcct gtttagcccc ggttttgtac gcccagtcga agaatttcag cacctctgtg
     7861 ccttgttctg gtttcttctg atctttgtgg atcagaatga acgtggtaga ggtaataggc
     7921 catgcatctt cgcctttctg gttggtcaga tcctgagcga aggttttgct ccagtctgca
     7981 ccttttgctg cattagcgaa gttttcttcg gtcggactaa ccggtttacc atcagcggag
     8041 atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag catattcaac ataaccaatt
     8101 gcacccggca gacgctgaac gaacgcggcg ataccgtcgt tacctttacc gcccagaccg
     8161 atcggccatt ttacggtaga gccagtacca acgttgtttt tccactcttc gttcactttc
     8221 gccaggtagc tggtgaagac gaaggaagtc ccggagccat ctgcgcggcg tactacagca
     8281 atgttttgtg aaggcagttt cagacccgga ttcagtttgg cgatggcttc atcatcccac
     8341 ttcttgattt tgcccaggta gatgtcgccg agggttttac catccagcac cagttcgcca
     8401 gacttcagcc ctggaatgtt aaccgccagc accacgccgc caatcacggt cgggaactgg
     8461 aacagacctt cctgagccag tttttcgtca gacagcggcg cgtcagaggc accaaaatca
     8521 acggtattag cgataatctg ttttacgcca ccggaagaac cgataccctg gtagttaact
     8581 ttattaccgg tttctttctg gtaagtgtca gcccatttgg catacaccgg cgcagggaag
     8641 gttgcacctg cacctgtcag gcttgcttct gcaaacacag agaaagcact catcgataag
     8701 gtcgcggcga caacagttgc gacggtggta cgcataactt tcataatgtc tcctgggagg
     8761 attcataaag cattgtttgt tggctacgag aagcaaaata ggacaaacag gtgacagtta
     8821 tatataagga atatgacatt tttatgacag aaaggaatta ccaaataact tactgatttt
     8881 aaatgtgaag atgaaaattc cacatgccat ctttttgtca tgatgcattt aaaagtgata
     8941 aagaaatggg tgagatgaca acgatgtgat gaacaatcgt tgtcagacct attagcgcac
     9001 aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc ggggctgggc gagctccagt
     9061 aaggctcgca ggagcagctg aaaaagttac actacctgat ttttcaccat acactgttga
     9121 tttatccatg tctgttggca actcaccacc gttcactttg atttccttat tatttttgtc
     9181 gtaaattttt atccccacgt tagagtttga agttacgacc attgaattgt ccgtattggg
     9241 ttcggtagca taaaaagacg tactgatatc atcggtattc agtatcccca ggttttcaca
     9301 ctcaatagtg actgttttag taattttatg tgttgttacc gcacttccaa caacagatga
     9361 aaaatctgca gctggtattt ctttaaaatc aaaacaaact ggctttaaat tatttatttc
     9421 gcaactttgc ggagctgtaa tatcgccccg aatataaaca tcagccatcg gctgtgagta
     9481 atccaccggg gtgtcacggc taattgttcc atacaatgtc actatctttg tttcaggaat
     9541 aatcaatttt cctgcaaatg tcttcttgat ataaaaatat actatcgctt tagccccact
     9601 ggatatactt aaaggttcaa tgcggttgca attgtacttt gtattaccag acgggttgtt
     9661 agcatgatat tcaaaaggtg cattcacata cccaacccca agaatatcta tagcaatccc
     9721 aacatcaagg ttcggattaa gatcataata attaatatta gagtgaaccc cagcataagt
     9781 taatgcctgg tttatttttg acgtataata gatatagggg aattctccta cgttaccagt
     9841 atcacaatga cagcgtccaa gatatgagga tccattagtt acagtggtgt taaatgtcgt
     9901 tccaacctta ttttcgttcg cggtaataag atatccc
//
LOCUS       DAJTMX010000135         9925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTMX010000135 DAJTMX010000000
VERSION     DAJTMX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.135"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..336
                     /locus_tag="OO145_003412"
     CDS             <1..336
                     /locus_tag="OO145_003412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468410.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            524..1639
                     /locus_tag="OO145_003413"
     CDS             524..1639
                     /locus_tag="OO145_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468411.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2318..2536
                     /locus_tag="OO145_003414"
     CDS             2318..2536
                     /locus_tag="OO145_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5468412.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /locus_tag="OO145_003415"
     CDS             2754..2996
                     /locus_tag="OO145_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468413.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO145_003416"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO145_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5468414.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO145_003417"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO145_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5468415.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO145_003418"
     CDS             complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO145_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5468416.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO145_003419"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO145_003419"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5468417.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="OO145_003420"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="OO145_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5468418.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2488 a         2397 c         2542 g         2498 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgac cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttc
//
LOCUS       DAJTMX010000136         9842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTMX010000136 DAJTMX010000000
VERSION     DAJTMX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.136"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            429..1547
                     /locus_tag="OO145_003421"
     CDS             429..1547
                     /locus_tag="OO145_003421"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5468419.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="OO145_003422"
     CDS             1613..1861
                     /locus_tag="OO145_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5468420.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO145_003423"
     CDS             1926..2294
                     /locus_tag="OO145_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5468421.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO145_003424"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO145_003424"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5468422.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO145_003425"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO145_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5468423.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO145_003426"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO145_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5468424.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO145_003427"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO145_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5468425.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO145_003428"
                     /pseudo
     CDS             complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO145_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2409 a         2728 c         2389 g         2316 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tattgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 cctatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc taatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 taaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac ggcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcggctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattc gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga
     9661 tttgtgattt tagtttggta cgcaatttgg tacacaacac tattttcact tcagcgggta
     9721 gtcatcaaac tcaccataac gagcatcgtt gatgatgtat gtgatcacca caaatatcac
     9781 atctggacta gttccgtctt catgcgcagg tagactggcc ccctgaatct ccagacaacc
     9841 ag
//
LOCUS       DAJTMX010000137         9830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTMX010000137 DAJTMX010000000
VERSION     DAJTMX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.137"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(84..824)
                     /gene="pflA"
                     /locus_tag="OO145_003429"
     CDS             complement(84..824)
                     /gene="pflA"
                     /locus_tag="OO145_003429"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5468426.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1016..3298)
                     /gene="pflB"
                     /locus_tag="OO145_003430"
     CDS             complement(1016..3298)
                     /gene="pflB"
                     /locus_tag="OO145_003430"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5468427.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3353..4210)
                     /gene="focA"
                     /locus_tag="OO145_003431"
     CDS             complement(3353..4210)
                     /gene="focA"
                     /locus_tag="OO145_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5468428.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4616..6376)
                     /locus_tag="OO145_003432"
     CDS             complement(4616..6376)
                     /locus_tag="OO145_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5468429.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6506..7132
                     /locus_tag="OO145_003433"
                     /pseudo
     CDS             6506..7132
                     /locus_tag="OO145_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7331..8419
                     /gene="serC"
                     /locus_tag="OO145_003434"
     CDS             7331..8419
                     /gene="serC"
                     /locus_tag="OO145_003434"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5468430.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8490..9773
                     /gene="aroA"
                     /locus_tag="OO145_003435"
     CDS             8490..9773
                     /gene="aroA"
                     /locus_tag="OO145_003435"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5468431.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2500 a         2355 c         2463 g         2512 t
ORIGIN      
        1 aagatgtgtt aaaaacgctg tagcagaatg aagcgcggaa taaaaaagcg gcaactcaat
       61 aaagttgccg ctttacgagg aaattagaac attaccttat gaccgtactg ctcaagaatg
      121 cctttcacgc gttccatggt ctctttcttc ggtggtttaa caccatcgag tttgtattct
      181 tcacccatcg ccacccattt gtgtttgccc agttcgtggt aggggagaag ttcgattttc
      241 tcaacgttac ccatatcacg ggtaaattca ccgaggcgat gtgccgaatc gtcatcgtca
      301 gaccagcctg ggacaacaac gtagcggatc cacaccttca catttttgtt cgccagatat
      361 ttagcgaact ccagcgtgcg gtggttggaa actccaacca gattttggtg gatctcgtcg
      421 ttcatctgtt tgagatcgag cattaccagg tcggttactt ccagcagttc atcaatcacc
      481 ggatcgtaac gacgaacaaa accgttggta tccagacagg tatgaatgcc ttctttttta
      541 caggcgcgga accagtcacg aacaaactca gcttgcagga ttgcttcacc gccggatgcg
      601 gtaacgccgc cgccggaagc gttcataaag tggcgatagg tcaccacttc cttcatcaaa
      661 tcttcaacgg taacttcttt accgccatgc gtgtcccagg tgtcgcggtt atgacaatac
      721 aggcagcgca tcaggcagcc ctggaaaaag gtgataaagc gaatacctgg gccgtctacg
      781 gttccacagg attcaaagga gtgaatgcga ccaataactg acattgcggt gtttctccag
      841 atgtggccca tctgaggccg tgttggtgcg cagctcgaag gctacgtcga gtctgttttg
      901 gcagtcacct taaagtatag atagctgaca aaaaaggctc tcgtgctaaa aaaggcccca
      961 ctttcgtgga gcctttattg tacgcttttt actgtacgat ttcagtcaaa tctaattaca
     1021 tagattgagt gaaggtacga gtaataacgt cctgctgctg ttctttagtc agcgagttga
     1081 aacgtactgc gtagccagat acacggatgg tcagctgcgg atatttttcc gggttttcca
     1141 tcgcgtcgag cagcatttca cggttcatca cgttaacgtt caggtgctga ccaccttcga
     1201 tggatgcttc gtggtggaag taaccatcca tcagaccagc caggttggtc ttacgaactt
     1261 cgtcgtcttt acccagtgcg ttcggaacga tagagaaggt gtaggagata ccatctttag
     1321 cgtaagcaaa cggcagttta gcaacggaag tcagagacgc tacagcacct ttctggtcac
     1381 gaccgtgcat cgggttagca cccggtccga acggcgcgcc agcacgacga ccgtctgggg
     1441 tgttaccagt tttcttacca tacacaacgt tagaagtgat ggtcagaaca gactgagtcg
     1501 ggatagcgtc acggtaggtg tgcagtttct gaattttctt catgaaacgt tctaccaggt
     1561 caacagccag gtcatctaca cgcggatcat tgttaccaaa ctgcgggtat tcgccttcga
     1621 tttcgaagtc gatagccaga ccgtcttcgt cacgaatcgg tttaactttc gcatatttga
     1681 ttgcagacag ggagtcagca gcaacggaca gaccagcgat accacacgcc atggtgcgga
     1741 taacgtcacg gtcgtgcagc gccatcagag aggcttcgta gctgtacttg tcgtgcatgt
     1801 agtggatgat gttcagtgca gtgatgtact gtttagccag ccagtccatg aagtgatcca
     1861 tgcgctccat cacttcatcg tagctcagga gatcgccttt gatcggttca gacttcggac
     1921 caacctgcat tttcagtttt tcgtcaacgc cgccgttgat tgcgtacagc atggttttcg
     1981 ccaggtttgc acgcgcaccg aagaactgca tttgtttacc aacgatcatc gggcttacgc
     2041 agcaagcgat agcgtagtca tcgttgttga agtccggacg catcaggtca tcgttctcat
     2101 actgcagaga agaggtgtcg atggacactt tagcggcgaa tttcttgaag ttcagcggca
     2161 gtttttcaga ccacagaatg gtcatgttcg gttccggaga cggccccata gtgtacaggg
     2221 tgttcaggaa acggaagctg tttttggtaa ccagggtacg accatcgagg cccataccac
     2281 cgatagattc ggttgcccag attgggtcgc cagagaacag ttcatcgtat tccggagtac
     2341 gcaggaagcg aaccatacgc agtttcatga ccaggtggtc aaccatttcc tgcgcttctt
     2401 gttcggtgat cttgccagct ttcaggtcac gttcgatgta cacatccagg aaggtggagg
     2461 tacgaccgaa ggacattgca gcaccgttct gagacttaac agcagccagg tagccgaagt
     2521 aagtccactg gatagcttcc tgagcgttgg tagccggacc agagatgtcg tagccgtatt
     2581 tcgcagccat ttctttcatc tgacccagag cgcggtgctg ttcagcgatt tcttcgcgca
     2641 gacggatagt ctgttccagg tttacgccgt tttccagatc agcctgtaga gaggtgaact
     2701 gagcgtattt gtctttcatc aggtagtcga taccgtacag cgcaacgcga cggtagtcac
     2761 cgatgatacg gccacggcca taagcatctg gcagaccggt cagaacaccg gatttacggc
     2821 aacgcaggat gtccggagtg taaacgtcga acacgccctg gttgtgagtt ttacggtatt
     2881 cagtgaagat ttttttgatc atcgggtcca gttcgcggtt gtacgctttg caggaacctt
     2941 cgatcatttt gataccaccg aacgggataa gagcacgttt cagcggagct tcagtctgca
     3001 gaccaacaac tttttccaac gctttattga tgtagccagc gtcgtgagag gtgatggtgg
     3061 aagcaacagc ggtgtcaaaa tcaactggcg cgtgagtgcg gttttccagt ttaacacctt
     3121 ccattacttt gtcccacagg gtggtggtcg cttcagtagc gccagccagg aaggactcgt
     3181 caccctcgta cggagtgtag tttttctgaa tgaagtcacg gacgtttact tcattctgcc
     3241 agtcaccttt ggtaaaacct tcccaggctg tggctaactt ttcattaagc tcggacatgt
     3301 aacacctacc ttcttaagtg gattttttat ttactgcgta cttcgacaac cattaatggt
     3361 gatcgttttc acgcaggtaa atgacccagt atgtcaaccc aaccaacaaa ccaccgccga
     3421 taatattacc gatcgtaacc gggatcaggt tatcagtgat gaagttcatc acggtcaggt
     3481 gagaaaaatt ttccggtgca gaaccgacag cggtccagaa ttccggagat gcgaagtcgc
     3541 ggattacaat acccatcggg atcataaaca tgtttgcgat actgtgctca aaaccgctgg
     3601 caacaaacat cgcgaccggc agcaccataa tgaacgcttt gtccatcagg ctgcggccag
     3661 aataactcat ccatactgcc agacatacca tcaggtttgc caggatacca agacagacgg
     3721 cctcaataaa agtatggtgc actttgtggt cggcggtttg taggacgttt agtccccatt
     3781 gaccatttgc ggtcatatac tcgccggaaa gccacattaa aagtacaaac agcagtgcgc
     3841 cgaccaggtt gccaaaatag acatttagcc agtttttagc caactgacac caggtgatgc
     3901 gcccactcgc cttagcaaca acaatcaaca ctgtggaagt aaagagatcg gctccgcaga
     3961 caacacaaag aatcagcccc agagagaagc aaatgccgcc aaccagtttt gccatgccga
     4021 agggcattgt gcctgtgcca gtggttgctg tgatatagaa gacgaatgcg attgagatga
     4081 aaacaccggc ggtaatcgcc agatagaaag tcttaagcgg atgtttcgtt gctttataga
     4141 cacccgcctc ttcggccact ttggccattg cagcaggaag taaaagatca aaagggttgt
     4201 cagctttcac actaactctc tctttattaa gtcggcgacg agatactaac aaagcattat
     4261 agatgagaaa ttgatataga tcatatctcg cctggcttat aggcccgtaa ctcgcatggt
     4321 ttttatgcaa atacggagta actatttgat tatccaaata aaaataaatt ttaaaaatta
     4381 acaaatgagt tgaatttttt ccgcatcctc cgctaaaaca gttaattaaa agggagcatc
     4441 aggcgaataa agtaacaata tcgatcgtat ttattaaata caaattaccg atatttaacc
     4501 ttataattac aattatttta ttaatgcaaa tatatgtaaa gcggggcatt aaaaaaacgc
     4561 cccgtaatat aactcagact aatcattaag cctacattgc gtaggctatt tgattttatt
     4621 ttgcccagaa tgctgctttg gcgcgctgca gcttttcgta ggccttcaac aacgactgat
     4681 gtgcagcaaa cgcgtgcaga tcgctatcta ccggttgcag gccgtaaaac gccgcttcgc
     4741 cgctcattgc cgcactggcg gcttctacgg catctgcgcc gtacatgcga acaaacgcat
     4801 tcagatattg cagcggctgg cgatcttctt cctgtgccag taataacaac gtttgcaggc
     4861 agcgataata gttggcgcgt tccggactaa atactgatga gttaaactcc atcgtccatt
     4921 cggtccagac cagagcctgt tccagatcgc caccagccag cgccagcatg gcttttaatt
     4981 caccgatacg cagggtgtac caaccgttat ccgacccggt cgccagaccc aacagctcac
     5041 gcacgcgggt aaagtcatca aaaccttctt catccagttg ctcgatgagg ttcaggtaat
     5101 cttctttttc ccactcgctg cctggtagcg aaagaatcgt ttcacgtaaa tggctgccca
     5161 tactgttatt cgcgagccac agatcttcag ccggataaat atcggacatg ccaggcacga
     5221 taatacggca agcataaacg cccagatgct cgtaatcggc aatataaact tctttatctt
     5281 ctttgttgaa gatagccatc agcgtagcaa actcttcttc cgtggtgccg gagaaattcc
     5341 agtccacaaa cggataatcg gcatcctgct tgaacaggtc ccaggagatt aaaccgctgg
     5401 aatcgataaa gtgcgtttcg aggttggtat gttcagcgac ttcttcatca tcgaaggttg
     5461 gcggagtaaa cacatccaaa tctttcaggc cacgaccttg cagcagctcg gtcacggtac
     5521 gttccagtgc tacgccaaaa tcaggatgcg caccgaaaga ggcaaagcag gtgccgtttg
     5581 caggattaaa cagtaccacg caaatcaccg gatactggcc gccaagggaa ccatcatatg
     5641 cgaagatcgg gaaaccttct gtttccagtg tttcgatcgc ttcaaccacc gctgggtaac
     5701 gcgccagcac gtctgccggg atttccggca agctgatgct ttcagcaata atgcggtttt
     5761 tcacgtagcg ttcgaaaact tcggacaacc cctgaacgcg tgcttcgttg cgggtattgc
     5821 cagcggacat accgttagaa acgtacaggt taccaatgat attcatcgga atataaacgg
     5881 tctgattgtc ggactggcgc gtaaacggca gaccgcaaat accacgatct tcgttaccgg
     5941 attgtaggtc aatcagcata ctgccggtca attcattctc cggatcgtaa aatgcgcgca
     6001 gacggtcatc gagcagccct tctggcacat cgtcattttc gctcagtggg aaccattttt
     6061 cgttgggata atgcacaaat ggaccgttgg cgatggtttc gcccagccag aaatcagcaa
     6121 aaaagtagtt ggttgagaga cgctcgaaat attcaccgag tgcagaagcc agcgccgctt
     6181 tcttggttgc gcctttaccg ttggtaaaac acagtgcgca ctctttgtcg cgaatatgta
     6241 cagaccagac gttaggcacg ggattcagcc aggaggcctc ttcaatctga aagccgaggt
     6301 ctgaaagttt ttgctggaag cgagcgatgg aatcttccag agcggcatct ttgccgggga
     6361 taaatgtttg cgtcatgaaa atcactttag tcgtacggaa agcgcgcaat aatacgggtt
     6421 ttatctcaaa ggcgctatca ccgccgccat gccgatgaac tgttgactat gcttttagcg
     6481 gataaaccac gttaagagca taaaaatgaa ggcgttcgat ctccaccgta tggcatttga
     6541 taaagtgcct tttgatttcc ttggcgaagt tgcactacgt agtctttata cctttgtact
     6601 ggttttttgt tcctcaaaat gaccggaaga cgcggtgtgc gacagatgtc gctgtttgaa
     6661 gtgttaatca ttctcacgct gggttcagcg gcgggagatg tggcgtttta tgatgatgtg
     6721 ccgatggtgc cggtacttat cgtctttatc actctggcgt tgttataccg cctggtaatg
     6781 tgatggcgag ctggcctggt cgaaactcaa taactccaac atgacggaat ttgagttctt
     6841 tatggagcta cgattgcgtg gcgtggagca gctggggcag gtacgtctgg cgattctcga
     6901 aaccaacggg caaatcagtg tctatttctt tgaagatgac aaggtgaaac cgggtttact
     6961 tattttaccc agtgattgta cgcagcgtta caaagtggtg ccggagtcgg cggactatgc
     7021 ctgcattcgt tgtagtgaaa tcattcatat gaacgcgggg gaaaaacaat tatgtccgcg
     7081 ctgtgcaaat ccagaatgga cgaaggcaag tcgggcaaaa cgggtgacct gacagtaaaa
     7141 acatcggctt tttgctaata atccgagaga ttcttttgtg tgatgcaagc cacatttttg
     7201 ccctcaacgg ttttactcat tgcgatgtgt gtcactgaat gataaaaccg atagccacag
     7261 gaataatgta ttacctgtgg tcgcaatcga ttgaccgcgg gttaatagca acgcaacgtg
     7321 gtgaggggaa atggctcaaa tcttcaattt tagttctggt ccggcaatgc taccggcaga
     7381 ggtgcttaaa caggctcaac aggaactgcg cgactggaac ggtcttggta cgtcggtgat
     7441 ggaagtgagt caccgtggca aagagttcat tcaggttgca gaggaagccg agaaggattt
     7501 tcgcgatctt cttaatgtcc cctccaacta caaggtatta ttctgccatg gcggtggtcg
     7561 cggtcagttt gctgcggtac cgctgaatat tctcggtgat aaaaccaccg cagattatgt
     7621 tgatgccggt tactgggcgg caagtgccat taaagaagcg aaaaaatact gcacgcctaa
     7681 tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg gttaagccaa tgcgtgaatg
     7741 gcaactctct gataatgcgg cttatatgca ttactgcccg aatgaaacca tcgacggtat
     7801 cgccatcgac gagacgccag acttcggcaa agatgtggtg gtcgccgccg acttctcttc
     7861 aaccattctt tcccgtccga ttgacgtcag ccgttatggt gtaatttacg ctggcgcgca
     7921 gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt cgtgaagatt tgctgggcaa
     7981 agcgaatatc gcgtgtccgt cgattctgga ttattccatc ctcaacgata acggctccat
     8041 gtttaacacg ccgccgacat ttgcctggta tctatctggt ctggtcttta aatggctgaa
     8101 agcgaacggc ggtgtagctg aaatggataa aatcaatcag caaaaagcag aactgctata
     8161 tggggtgatt gataacagcg atttctaccg caatgacgtg gcgaaagcta accgttcgcg
     8221 gatgaacgtg ccgttccagt tggcggacag tgcgcttgac aaattgttcc ttgaagagtc
     8281 ttttgctgca ggccttcatg cactgaaagg acaccgtgtg gtcggcggaa tgcgcgcgtc
     8341 tatttataat gccatgccgc tggaaggcgt taaagcgctg acagacttca tggttgagtt
     8401 cgaacgccgt cacggttaat gccgaaattt tgcttaatcc ccacagccag cctgtggggt
     8461 ttttatttct gttgtagaga gttgagttca tggaatccct gacgttacaa cccatcgctc
     8521 gtgtcgatgg cactattaat ctgcccggtt ccaagagcgt ttctaaccgc gctttattgc
     8581 ttgcggcatt agcacacggc aaaacagtat taaccaatct gctggatagc gacgacgtgc
     8641 gccatatgct gaatgcatta acagcgttag ggttaagcta tacgctttca gccgatcgta
     8701 cgcgttgcga aattatcggt aacggcggtc cattacacgc agaaggtgcc ctggagttgt
     8761 tcctcggtaa cgccggaacg gcaatgcgtc cgctggcggc agctctttgt ctggatagca
     8821 atgatattgt gctgaccggt gagccgcgta tgaaagagcg cccgattggt catctggtgg
     8881 atgcgctgcg cctgggcggg gcgaagatca cttacctgga acaagaaaat tatccgccgt
     8941 tgcgtttaca gggcggcttt actggcggca acgttgacgt tgatggctcc gtttccagcc
     9001 aattcctcac cgcactgtta atgactgcgc ctcttgcgcc ggaagatacg gtgattcgta
     9061 ttaaaggcga tctggtttct aaaccttata tcgacatcac actcaatctg atgaagacgt
     9121 ttggtgttga aattgaaaat cagcactatc aacaatttgt cgtaaaaggc gggcagtctt
     9181 atcagtctcc gggtacttat ttggtcgaag gcgatgcatc ttcggcttct tacttcctgg
     9241 cagcagcagc aatcaaaggc ggcactgtaa aagtgaccgg tattggacgt aacagtatgc
     9301 agggtgatat tcgctttgct gatgtgctgg aaaaaatggg cgcgaccatt tgctggggcg
     9361 atgattatat ttcctgcacg cgtggtgaac tgaacgctat tgatatggat atgaaccata
     9421 ttcctgatgc ggcgatgacc attgccacgg cggcgttatt tgcaaaaggc accaccacgc
     9481 tgcgcaatat ctataactgg cgtgttaaag agaccgatcg cctgtttgcg atggcaacag
     9541 aactgcgtaa agtcggcgcg gaagtggaag aggggcacga ttacattcgt atcactcctc
     9601 cggaaaaact gaactttgcc gagatcgcga catacaatga tcaccggatg gcgatgtgtt
     9661 tctcgctggt ggcgttgtca gatacaccag tgacgattct tgatcccaaa tgcacggcca
     9721 aaacatttcc ggattatttc gagcagctgg cgcggattag ccaggcagcc tgaatgaaca
     9781 acgggcaata aatagccaaa tctttcttta tcaaaacgtc ggcacattgt
//
LOCUS       DAJTMX010000138         9774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTMX010000138 DAJTMX010000000
VERSION     DAJTMX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.138"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO145_003436"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO145_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5468432.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRD
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO145_003437"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO145_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5468433.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO145_003438"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO145_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5468434.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO145_003439"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO145_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5468435.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO145_003440"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO145_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5468436.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO145_003441"
     CDS             complement(6031..6360)
                     /locus_tag="OO145_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5468437.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO145_003442"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO145_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5468438.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO145_003443"
     CDS             7998..8171
                     /locus_tag="OO145_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5468439.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO145_003444"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO145_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5468440.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2562 a         2285 c         2434 g         2493 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt gacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggtac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAJTMX010000139         9678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTMX010000139 DAJTMX010000000
VERSION     DAJTMX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.139"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(26..214)
                     /locus_tag="OO145_003445"
     CDS             complement(26..214)
                     /locus_tag="OO145_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5468441.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO145_003446"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO145_003446"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5468442.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO145_003447"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO145_003447"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5468443.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO145_003448"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO145_003448"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5468444.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO145_003449"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO145_003449"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5468445.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO145_003450"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO145_003450"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5468446.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6239
                     /locus_tag="OO145_003451"
     CDS             5109..6239
                     /locus_tag="OO145_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5468447.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
     gene            6328..6861
                     /gene="blc"
                     /locus_tag="OO145_003452"
     CDS             6328..6861
                     /gene="blc"
                     /locus_tag="OO145_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5468448.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6858..7175)
                     /gene="sugE"
                     /locus_tag="OO145_003453"
     CDS             complement(6858..7175)
                     /gene="sugE"
                     /locus_tag="OO145_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5468449.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7351..7497)
                     /gene="ecnB"
                     /locus_tag="OO145_003454"
     CDS             complement(7351..7497)
                     /gene="ecnB"
                     /locus_tag="OO145_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5468450.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7608..7733)
                     /gene="ecnA"
                     /locus_tag="OO145_003455"
     CDS             complement(7608..7733)
                     /gene="ecnA"
                     /locus_tag="OO145_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5468451.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7785..8351)
                     /gene="efp"
                     /locus_tag="OO145_003456"
     CDS             complement(7785..8351)
                     /gene="efp"
                     /locus_tag="OO145_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5468452.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8393..9421
                     /gene="epmB"
                     /locus_tag="OO145_003457"
     CDS             8393..9421
                     /gene="epmB"
                     /locus_tag="OO145_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5468453.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2466 a         2478 c         2465 g         2269 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aattattcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg cctgtcagat tctcaacgct ctacagtaaa
     6241 atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt catctacact
     6301 tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca
     6361 gctgcatttc tggtcgttgc ctgtagtttt cctacgccgc cgcgtggcgt gaccgtagtg
     6421 aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg ttttgatcac
     6481 cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga tgacggcggc
     6541 ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca gagtgaaggg
     6601 aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt ctttggtcct
     6661 ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc gctggtttgc
     6721 ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc tgacgaagtg
     6781 aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg
     6841 gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc caatacgatt
     6901 aacgccagac tcgccagacg catcgggtta gcggactcac cgagcagcac aatgccggtg
     6961 atggccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc taccggtaac
     7021 gatttcatcg cccaggcaag tagcgccata ctgactatca tcgccgtcac ggtaataaca
     7081 ctcggcgtca aacggctaaa gccgtgggta tatttcaggc caacggccca taccacttcc
     7141 agcagaccag caataactaa gataatccag gacatatcag gctccggaaa tttggggccg
     7201 tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag ctgatggttg aacggatatt
     7261 ttgccaggtg gcaggggaat ttcaactatt acctttatga cgatcgcaga aaaaacaaac
     7321 ggcacgacac tgctgtcgta ccgttattgc ttattgctgc gctttcgttg ctgcgccaga
     7381 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
     7441 taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac
     7501 gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat
     7561 cgccaaaaat gatggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg
     7621 cgagagatgg agttaccgag atgtttgatg tcttcgccga aaccgcaagc ggtgttacag
     7681 cccgtgagca gcgtgctggc aagcaaaaca agaacgataa ggcgtttcat catctctgac
     7741 ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag
     7801 acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac gaacagcgga
     7861 actttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca
     7921 cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg cggagtaacg
     7981 gagatcggct gaccattcca cagagttacg atacactctg cctgatccag cagccattta
     8041 gcattgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg
     8101 aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagcg
     8161 ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga
     8221 cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca
     8281 accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag
     8341 tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca
     8401 tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt
     8461 gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc
     8521 cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat
     8581 ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc aagatgagtt
     8641 tgtcgtcgcg tccggattct ccaccgaccc actggaagaa cagcacagcg tagtgcctgg
     8701 tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa
     8761 ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa
     8821 ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc
     8881 cggcggcgat ccgctgatgg cgaaagagca cgagctggac tggttgctca cacaactgga
     8941 agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg tgatcccggc
     9001 acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct acgctgcaaa tcttgctggt
     9061 gaatcacatc aatcatgcca atgaggtaga tgaaacgttc cgtcaggcga tggctaaatt
     9121 gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg ttacgtggtg tgaacgataa
     9181 cgcacaaacg ttggcaaacc tgagtaatgc gttgttcgat gccggcgtga tgccgtatta
     9241 cctgcatgtg ctagataaag tacagggcgc ggcgcatttt atggtgagtg atgacgaagc
     9301 acggcagatt atgcgtgagt tgctgacatt ggtgtcggga tatctggtgc cgaaactggc
     9361 gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagta
     9421 actgattaaa cagggaagtt aaacattaaa attgcctgat gcgctacgct tatcaggcct
     9481 acatcgtgca ttgaaattga atggatttgc aatgaactgt aggccggata aggcgtttac
     9541 gccgcatccg gcaagagcaa caaacgcaag tacagcaacc actgagaaat ttaatctttg
     9601 cacgttgcat aacctttaat tattcatatg cattgctgtt gttacttttt atatagctaa
     9661 caccaataat taaccttt
//
LOCUS       DAJTMX010000140         9594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTMX010000140 DAJTMX010000000
VERSION     DAJTMX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.140"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO145_003458"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO145_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5468454.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO145_003459"
     CDS             complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO145_003459"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5468455.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO145_003460"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO145_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5468456.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2403..2606)
                     /locus_tag="OO145_003461"
     CDS             complement(2403..2606)
                     /locus_tag="OO145_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468457.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO145_003462"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO145_003462"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5468458.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO145_003463"
     CDS             complement(3802..4014)
                     /locus_tag="OO145_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5468459.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO145_003464"
     CDS             4274..4582
                     /locus_tag="OO145_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5468460.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO145_003465"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO145_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5468461.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO145_003466"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO145_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5468462.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO145_003467"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO145_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5468463.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO145_003468"
     CDS             complement(8478..8981)
                     /locus_tag="OO145_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5468464.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9069..>9594)
                     /locus_tag="OO145_003469"
     CDS             complement(9069..>9594)
                     /locus_tag="OO145_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5468465.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2380 a         2446 c         2285 g         2483 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagcttgtt gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgtgcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgctag
      901 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacat cggttgacca ggcggttgct gatgcagccg agtcggtgac
     1501 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacaa ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ctgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttgccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagtagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggt gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt
     9061 gaagaatatt accaagtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     9121 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     9181 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     9241 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     9301 gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     9361 ctgtcgtaag gctgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     9421 tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     9481 tcgtaaccgg ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     9541 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caag
//
LOCUS       DAJTMX010000141         9522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTMX010000141 DAJTMX010000000
VERSION     DAJTMX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.141"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..593)
                     /locus_tag="OO145_003470"
     CDS             complement(<1..593)
                     /locus_tag="OO145_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5468466.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="OO145_003471"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="OO145_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5468467.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="OO145_003472"
     CDS             complement(1422..1961)
                     /locus_tag="OO145_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5468468.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="OO145_003473"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="OO145_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5468469.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="OO145_003474"
     CDS             complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="OO145_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5468470.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="OO145_003475"
                     /pseudo
     CDS             complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="OO145_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6185..6901
                     /gene="nanC"
                     /locus_tag="OO145_003476"
     CDS             6185..6901
                     /gene="nanC"
                     /locus_tag="OO145_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5468471.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6921..8027
                     /gene="nanM"
                     /locus_tag="OO145_003477"
     CDS             6921..8027
                     /gene="nanM"
                     /locus_tag="OO145_003477"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5468472.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8092..9072
                     /locus_tag="OO145_003478"
     CDS             8092..9072
                     /locus_tag="OO145_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5468473.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2787 a         2077 c         1996 g         2662 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg
     3001 gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac
     3061 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
     3121 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
     3181 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
     3241 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
     3301 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
     3361 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
     3421 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
     3481 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
     3541 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
     3601 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
     3661 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
     3721 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta
     3781 aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt
     3841 caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag
     3901 tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt
     3961 tgaagaacat aaatcattag attttgtatg ggtatggtta acttaatgtt tattatgata
     4021 attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt ttgatatatc
     4081 gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc
     4141 atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc
     4201 ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt
     4261 atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat
     4321 ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg
     4381 agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga
     4441 agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt
     4501 tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc
     4561 cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat
     4621 cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat
     4681 ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc
     4741 ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct
     4801 gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct
     4861 gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat
     4921 gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc
     4981 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     5041 aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga
     5101 cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt
     5161 agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag
     5221 cttaggccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga
     5281 cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc
     5341 tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     5401 aacaccaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca
     5461 aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta
     5521 ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat
     5581 tatttataaa acgtatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt
     5641 cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa
     5701 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     5761 agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc
     5821 agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga
     5881 tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta
     5941 cgaatattca aatcaattaa aaatcagaag agattttata ctcaggtatt taatctggat
     6001 ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata
     6061 taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac
     6121 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
     6181 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
     6241 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
     6301 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
     6361 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
     6421 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
     6481 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
     6541 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
     6601 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     6661 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga
     6721 ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca
     6781 tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc agggtgttta
     6841 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     6901 gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt
     6961 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     7021 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca
     7081 agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac
     7141 cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg
     7201 gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     7261 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt
     7321 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     7381 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc
     7441 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     7501 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     7561 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     7621 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     7681 atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     7741 ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     7801 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     7861 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     7921 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     7981 cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     8041 cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca
     8101 ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     8161 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa
     8221 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt
     8281 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     8341 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
     8401 ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg
     8461 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     8521 tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca
     8581 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     8641 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     8701 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
     8761 tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat
     8821 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     8881 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     8941 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     9001 acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg
     9061 cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga
     9121 caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc
     9181 aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt
     9241 attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg
     9301 gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac
     9361 cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat
     9421 gatatgctta atgatgttca aagaaattag gccattatta tattattgct tgcattttct
     9481 ttcattccta aaagcacccg ccattaatcc caggcgcatt at
//
LOCUS       DAJTMX010000142         9488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTMX010000142 DAJTMX010000000
VERSION     DAJTMX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.142"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..189)
                     /locus_tag="OO145_003479"
     CDS             complement(<1..189)
                     /locus_tag="OO145_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5468474.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            709..1404
                     /gene="minC"
                     /locus_tag="OO145_003480"
     CDS             709..1404
                     /gene="minC"
                     /locus_tag="OO145_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5468475.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1428..2240
                     /gene="minD"
                     /locus_tag="OO145_003481"
     CDS             1428..2240
                     /gene="minD"
                     /locus_tag="OO145_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5468476.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2244..2510
                     /gene="minE"
                     /locus_tag="OO145_003482"
     CDS             2244..2510
                     /gene="minE"
                     /locus_tag="OO145_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5468477.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2882..3226)
                     /locus_tag="OO145_003483"
                     /pseudo
     CDS             complement(2882..3226)
                     /locus_tag="OO145_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3261..3380)
                     /locus_tag="OO145_003484"
     CDS             complement(3261..3380)
                     /locus_tag="OO145_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5468478.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3341..3526)
                     /locus_tag="OO145_003485"
     CDS             complement(3341..3526)
                     /locus_tag="OO145_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468479.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3627..3800
                     /locus_tag="OO145_003486"
     CDS             3627..3800
                     /locus_tag="OO145_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468480.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3802..4146
                     /locus_tag="OO145_003487"
     CDS             3802..4146
                     /locus_tag="OO145_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5468481.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4150..4485
                     /locus_tag="OO145_003488"
     CDS             4150..4485
                     /locus_tag="OO145_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5468482.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4542..6863)
                     /locus_tag="OO145_003489"
     CDS             complement(4542..6863)
                     /locus_tag="OO145_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5468483.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6980..7189)
                     /locus_tag="OO145_003490"
     CDS             complement(6980..7189)
                     /locus_tag="OO145_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468484.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7589..7804)
                     /locus_tag="OO145_003491"
                     /pseudo
     CDS             complement(7589..7804)
                     /locus_tag="OO145_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7936..9459)
                     /locus_tag="OO145_003492"
     CDS             complement(7936..9459)
                     /locus_tag="OO145_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5468485.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2657 a         2205 c         2078 g         2548 t
ORIGIN      
        1 ggctttttcg ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca
       61 aatatacggg tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc
      121 agacgccatt gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat
      181 catattcata gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca
      241 atccgtaatc aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg
      301 tcttctgcgg tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg
      361 ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac
      421 acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg
      481 aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg caatgggttg attgacaagg
      541 gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa
      601 atgggagggt gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa
      661 catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacact
      721 ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa
      781 cctaaggtta tccatcaggc gctgaaagac aaaatcgctc aggcccccac atttttaaaa
      841 catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg
      901 cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg
      961 caacttaaag ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg aaaagaaaaa
     1021 gcgccacgtc cagctcccgc accgcaggct ccagcgcaaa atacaatgcc ggtcacaaaa
     1081 acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat
     1141 ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat
     1201 gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa
     1261 atattttgta cgaacctgat ggcggaattg gtgtccatcg caggtgaata ctggctgagt
     1321 gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct
     1381 ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta
     1441 ttgttgttac ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca
     1501 ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta
     1561 atctcgacct gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc aacgtcattc
     1621 agggcgatgc aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata
     1681 ttcttccggc atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag
     1741 ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga
     1801 ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc
     1861 cggaagtctc ctcagtacgc gactctgacc gtattttagg cattctggcg tcaaaatcac
     1921 gccgcgcaga aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc
     1981 caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca
     2041 tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct aaccagggtg
     2101 aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac
     2161 gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca
     2221 aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaga
     2281 acacagccaa cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg
     2341 atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat
     2401 acgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt
     2461 ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta
     2521 aaaacgcatt tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc
     2581 cacgttgtgg taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc
     2641 ccctttacac cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta
     2701 aataaccatg ttattagtgt gtatatactg ggagatgata accccgcaat ttcattattc
     2761 attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa
     2821 ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg
     2881 gtcagaacga ccagttcaca ccagccaccg cgttccacgg ggactccaca ccggcaccat
     2941 ggctataccc caccccgaaa tgcccgctta acgtactgct gaatgaggct ttaatacctg
     3001 cctggtatat tccacgtctg cccgacaaat cattgacaaa attaccgtca ctattcactt
     3061 tcacccgatt atcatcgaca aattctttgc gcacagccgc cttcaggcag ggttcaattt
     3121 ccataccgtt ccccagacgc atgttgtaac tcagcgttgc acccagttca cgatatatac
     3181 tgcgggtatc gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg
     3241 acctaagctc tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga
     3301 gacagcgcag gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat
     3361 cccgcataag aaagaggcat tttttacacg cagtgcgctg aagtgtagtt ggataaaaaa
     3421 tcactctttt cagataatac tgtcgtacgc ttatctttag acaatgaggc cgtcggaaaa
     3481 ataaaatgac taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca
     3541 ataatattga taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac
     3601 ttcttgaata aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc
     3661 acgtaaaaaa cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata
     3721 gcgagcgttc tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt
     3781 acttaatacc ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca
     3841 ttgttctgct ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta
     3901 ggcgcaggcg ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct
     3961 gttggcgctg gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa
     4021 ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc
     4081 gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg
     4141 aaataaggaa tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca
     4201 atgacctggg cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact
     4261 tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg
     4321 gtaatgggta atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg
     4381 agcgccaaag tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg
     4441 atcacggcaa ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc
     4501 gcgtcgatga atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg
     4561 cccaccatca ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc
     4621 cagacgctgg ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta
     4681 ctcaccccat cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta
     4741 tggatccagt tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc
     4801 tcctgagtac gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca
     4861 ttctcaatac gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata
     4921 gcctgtgcct gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta
     4981 ttcagggcaa aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat
     5041 tcataggcta attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag
     5101 tcaatgtatg cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg
     5161 ctatagcctc tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg
     5221 tagtcgcggg cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca
     5281 ccggttaaca gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt
     5341 ccgttgctgt cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa
     5401 cgctcctgtt cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc
     5461 ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc
     5521 aggacggggg taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg
     5581 ctcgttgcct ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg
     5641 gttaatgaat attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt
     5701 gattccccct caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct
     5761 gcattggtaa ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc
     5821 gtagctgaat tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg
     5881 taattttcag ttattgttaa tcttgttggt tcgacatccc gtccatctgc ccgggaaata
     5941 tagaccgtac tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag
     6001 gtactattat ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct
     6061 gaacccgtta tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata
     6121 aaaacatctg cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct
     6181 ttatggtaga ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt
     6241 gcttcctgac catttatgga agactccagc acaattccag acccctgatg cgcgactgca
     6301 gatgtatttt tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga
     6361 cccgttgtat aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca
     6421 tgagcattgt ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta
     6481 taatcaagcg tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct
     6541 gttccgtggg ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca
     6601 ttacttcctg tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt
     6661 ccgggagatg cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta
     6721 tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg
     6781 tcagtattca ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg
     6841 acaatcgtgt ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta
     6901 gctgttaatg ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca
     6961 atgacgccat tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc
     7021 cattacccgt tgttataact agcgcattat caatatcttt tacttttcca tcttttattg
     7081 ccacagcatg tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg
     7141 ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat
     7201 ccattcattg gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa
     7261 cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat
     7321 ataaatgata aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact
     7381 ttggggtgac tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc
     7441 atggtaatta tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat
     7501 ttgcgcaata attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc
     7561 cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt
     7621 ctcttaaatc gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc
     7681 gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac caggaggtca
     7741 gcataaatgc aatggaaaag atgatataga gagtgaaata atccagatta ttactattat
     7801 tcatgctact aatggcaatc ctgtcaaaac tttcaaacaa ttatagcaaa caaaataatg
     7861 aacaatagta acaagaggca acgagctaat tagctgtcta taattacacc gcactatggc
     7921 cttactgatc aattttcact caaccacaac cttcaccttc ggtttagaaa ggataatttt
     7981 gaccaggtcg atgtatgtaa caggtttata gaaataataa ccctgctgaa atgtgatatt
     8041 attttggttc agatagccaa gttgttcttt cgtttcgaca ccttcagcga cgatactgat
     8101 cgaaagttta cgcgccagat ccaatacaca atccagaatt cgggttgatt ctgggtctgc
     8161 gtttacgcgg ccaacgaaac tgtgatcaat tttgatataa tcaatatgca ggtcatgaag
     8221 ataagaaagc cctgagtagc cagtaccgaa atcatccagc gcgatgacaa aaccattttc
     8281 atgcagaatg ttcaaccgct gaaccagact ttcatcaaca ttcaatggct cacgctcggt
     8341 gacttccaga acaaggttta aatcgcggcg ggtaaaactg tcacggaaat ttaaacactc
     8401 gtcgacaaac gtcggcgaaa taatatgcga agcgctaaaa ttaattccaa tatgaaaacc
     8461 ttccggcagt ttactcgcga tagcgttcat ctgtctggca acctgattca tcaggctttg
     8521 tgtaagcgga acgattaatc cggatttttc agcaagtgga ataaatgccg cgggtgaaat
     8581 atatccaccg tgaggttgtt tccagcgggc taacacctca actccccgca atgtcccttc
     8641 ccgaccattt accacaggtt ggtaaaaagg gacgatctcc ccattattta ttgcccgtcg
     8701 caggatctct tcaggcgttg cgcttttatt gaaatatcta tcaagcagga aggcagcggc
     8761 gctggcgatt aacagaataa atattaaaat cccaaagcct ttatcgataa gtctgtagaa
     8821 gctaaagagt ggcggcgggt taaagataat agtgaaatga tattttgtgg agtgaacctt
     8881 ctcggtatag ggataatgtc cattaagcgt catcacatca gccgttggtc caataatgtc
     8941 atccgccacg cgtaatacat accttacccc tttcaagggt acattaagcg ccccgcgaat
     9001 atgttgatca cttatcgtaa ccaaaatgcg cgtatctgca aattggctct gatagagcag
     9061 gataggtaat ctattaacgg tgtcgattgc cggagccaac agtaaattac tgtccgggac
     9121 aacaggaata cgagacagca ggacccgatt cccaggcagg gatgtacacc agactattcc
     9181 ctgtttgaga atgatgattg tgcgcaggtg aggtttcaga gctgcttcag tgccaagctg
     9241 atattgctcc tcgaggtcgc attccttccc ggcaatgtta accgctgttt gagtcgcctg
     9301 tgacgcttct tcaagtatat gattgatatt gttggcggca tatcttgctc ccgccaggta
     9361 ctctgctttt gcggaatacc agagctggat gttcaaaata gcgacaccgg taataaacaa
     9421 gcatatcgca acgaggatgg gtatgagtgt attgcgcatt tgcttgtccc ggccaaaggg
     9481 tttaatac
//
LOCUS       DAJTMX010000143         9469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTMX010000143 DAJTMX010000000
VERSION     DAJTMX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.143"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..117
                     /gene="truD"
                     /locus_tag="OO145_003493"
                     /gene_synonym="ygbO"
     CDS             <1..117
                     /gene="truD"
                     /locus_tag="OO145_003493"
                     /gene_synonym="ygbO"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="catalyzes the modification of U13 in tRNA(Glu);
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5468486.1"
                     /translation="WWDDVTVEIRFWLPAGSFATSVVRELINTTGDYAHIAE"
     gene            98..859
                     /gene="surE"
                     /locus_tag="OO145_003494"
     CDS             98..859
                     /gene="surE"
                     /locus_tag="OO145_003494"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5468487.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            853..1479
                     /gene="pcm"
                     /locus_tag="OO145_003495"
     CDS             853..1479
                     /gene="pcm"
                     /locus_tag="OO145_003495"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5468488.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            1619..2758
                     /gene="nlpD"
                     /locus_tag="OO145_003496"
     CDS             1619..2758
                     /gene="nlpD"
                     /locus_tag="OO145_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5468489.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            2821..3813
                     /gene="rpoS"
                     /locus_tag="OO145_003497"
     CDS             2821..3813
                     /gene="rpoS"
                     /locus_tag="OO145_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5468490.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(3933..4340)
                     /locus_tag="OO145_003498"
     CDS             complement(3933..4340)
                     /locus_tag="OO145_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5468491.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            4487..5080
                     /locus_tag="OO145_003499"
     CDS             4487..5080
                     /locus_tag="OO145_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5468492.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            5080..6027
                     /locus_tag="OO145_003500"
                     /pseudo
     CDS             5080..6027
                     /locus_tag="OO145_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(6300..6863)
                     /locus_tag="OO145_003501"
     CDS             complement(6300..6863)
                     /locus_tag="OO145_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468493.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            7028..7279
                     /locus_tag="OO145_003502"
     CDS             7028..7279
                     /locus_tag="OO145_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5468494.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            7281..9377
                     /locus_tag="OO145_003503"
     CDS             7281..9377
                     /locus_tag="OO145_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5468495.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         2393 a         2314 c         2544 g         2218 t
ORIGIN      
        1 tggtgggatg acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc
       61 agcgttgtca gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatta
      121 tgacggggta catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga
      181 cgttcaggtg gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga
      241 atcctccctg cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc
      301 gaccgattgt gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt
      361 gtccggaatt aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc
      421 cgccgcgatg gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca
      481 taaacattac gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga
      541 gccgctgcgc accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat
      601 caaaggtatt cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca
      661 gcaagatccg cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc
      721 cggtccgggg accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca
      781 tgtggattta actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg
      841 agttggcacg caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa
      901 ggtattcagg atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt
      961 gatgaagcgt ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag
     1021 acaatttcgc agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag
     1081 tcgcgggtgc tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt
     1141 gtccagcatg tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc
     1201 ctgaaaaatc ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg
     1261 caggcacgtg cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact
     1321 gcgctaatga cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac
     1381 cagtatttga aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc
     1441 gtgcgctttg tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat
     1501 ttttcctggt tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta
     1561 gggtgccttg cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat
     1621 gagcgcggga agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct
     1681 atggctggca ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg
     1741 caatgcgcct gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac
     1801 gacgtctaca gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac
     1861 tcaacaaccg caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa
     1921 cggacgcatc gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag
     1981 tacctatacc gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga
     2041 tttccgtgac cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca
     2101 gaccttgcag gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca
     2161 ggccgacgca gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt
     2221 tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat
     2281 gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc
     2341 aagcacaacc gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg
     2401 ctggccgact gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg
     2461 gattgatatc gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt
     2521 ttatgctggt aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga
     2581 ttacctgagt gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa
     2641 ggcggggcaa aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt
     2701 tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat
     2761 cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt
     2821 atgagtcaga atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac
     2881 ggagttgagg tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg
     2941 gccgaagagg aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt
     3001 taccttggtg agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg
     3061 cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt
     3121 ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc
     3181 gaagagggca acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc
     3241 cgcttctcaa catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac
     3301 caaacccgta ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga
     3361 accgcacgtg agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag
     3421 caactggata agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc
     3481 tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat
     3541 gaaaaagaga acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc
     3601 aaatggctgt tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg
     3661 ctggggtatg aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa
     3721 cgtgttcgcc agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag
     3781 gggctgaata tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct
     3841 caagcgaggc tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag
     3901 acacgtcagc gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac
     3961 tgcataaaca gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg
     4021 tcaccaatag gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag
     4081 cgtcgccgct tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt
     4141 gccaacattt ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt
     4201 tcaataccag gtttatcagc aatggcgcgc atcaccgcgt actgtggttt ggtcaggtca
     4261 ggtaactcgt gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac
     4321 gctttatttc gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt
     4381 ttttagtgac gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag
     4441 tatgttaata ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt
     4501 cgggatgaca ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga
     4561 gatgccgaat gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact
     4621 ggaaacgcct tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc
     4681 ggatcaggcg gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc
     4741 gtgcagtatg aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg
     4801 cgcggcggac gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc
     4861 gcttagcacc atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt
     4921 gccgccgatg cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt
     4981 ggtagcccgc gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg
     5041 attgccgcag gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc
     5101 agctttttac aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac
     5161 gctgagccgg atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc
     5221 gcgctgtggt ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc
     5281 ggttcctggc agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag
     5341 cagattgatg agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc
     5401 aatccagcct gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg
     5461 ccgctgtttc gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt
     5521 tcccgcgatc cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg
     5581 gaagtgaagg gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg
     5641 catctgcata aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac
     5701 gatccgatca tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa
     5761 atggcaggcg cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt
     5821 gatgtgccgt ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa
     5881 atcgaagggc cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg
     5941 gtgcgcatcg ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt
     6001 atgccgtgga ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag
     6061 agattaaaaa agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga
     6121 taaagcgagt ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac
     6181 aggttcttac aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc
     6241 tgatgggtgc aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt
     6301 tatgcgctgg ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg
     6361 taaatctctc taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga
     6421 actccaacta gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc
     6481 gctccttttc tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac
     6541 aagaacatta gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa
     6601 tcttttagtt caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt
     6661 tctgctatct tttttaactg atacgggcgt ggtatcggtg cgtaatcccg cctgttgtaa
     6721 taatttttta aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg
     6781 tatcgttcta ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa
     6841 gaaccttggg gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat
     6901 aaaattaaaa aataaaccat aagaacacca aagaaccctt tgcagggaat ctttggtatc
     6961 ttaatcttta tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa
     7021 aggaaagatg agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct
     7081 ggcgctcaaa gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc
     7141 tcaggattca ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc
     7201 tgacgctata ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt
     7261 tatcaggaag gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac
     7321 caggaatgcc agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc
     7381 ttggtgacgt tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc
     7441 tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag
     7501 agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc
     7561 aggcaatcga agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga
     7621 ccgaaaacca gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc
     7681 tggaaagcgc cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc
     7741 gtaaaggtga gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg
     7801 gcgctaatcg tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt
     7861 cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac
     7921 cggaagaaat cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac
     7981 ccatgtcaga agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac
     8041 cgtacatgct ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc
     8101 cggaagtggt gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat
     8161 tcacccgccg cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg
     8221 gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt
     8281 ttgtgatcga tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg
     8341 tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt
     8401 attactcgga taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta
     8461 ttctgccacg cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg
     8521 gcattattga acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct
     8581 attttggcaa gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag
     8641 cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg
     8701 agctgccatc gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca
     8761 accgcccgca ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt
     8821 tcagaaaaaa acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc
     8881 tgcgcgacat gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc
     8941 tgaacaatat ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca
     9001 gttatgacat tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct
     9061 gtgaggcgat atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca
     9121 aacaacagca gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg
     9181 aagatgaagg tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt
     9241 atcgcccggt tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg
     9301 aagaatttga acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaagaag
     9361 atgatccgct ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa
     9421 caaagctgat ttgaaataac cgaggaaata ctatgactga tattgatga
//
LOCUS       DAJTMX010000144         9304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTMX010000144 DAJTMX010000000
VERSION     DAJTMX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.144"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            552..1343
                     /locus_tag="OO145_003504"
     CDS             552..1343
                     /locus_tag="OO145_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468496.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1729..1935
                     /locus_tag="OO145_003505"
     CDS             1729..1935
                     /locus_tag="OO145_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5468497.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2021..2593
                     /locus_tag="OO145_003506"
     CDS             2021..2593
                     /locus_tag="OO145_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468498.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2595..2879)
                     /locus_tag="OO145_003507"
     CDS             complement(2595..2879)
                     /locus_tag="OO145_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468499.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2850..2993
                     /locus_tag="OO145_003508"
                     /pseudo
     CDS             2850..2993
                     /locus_tag="OO145_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4162..5181
                     /locus_tag="OO145_003509"
     CDS             4162..5181
                     /locus_tag="OO145_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5468500.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5291..6163
                     /locus_tag="OO145_003510"
     CDS             5291..6163
                     /locus_tag="OO145_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5468501.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            6381..8765
                     /locus_tag="OO145_003511"
     CDS             6381..8765
                     /locus_tag="OO145_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5468502.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            8762..>9304
                     /locus_tag="OO145_003512"
     CDS             8762..>9304
                     /locus_tag="OO145_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5468503.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALE"
BASE COUNT         2720 a         2170 c         2114 g         2300 t
ORIGIN      
        1 agacattact taacaaagaa cattacagtc agtgtgtaca ttacagattt atattcaggt
       61 gttgcgggac tacattacag cagagagggg taaggacaga gttacaagta ctactagaga
      121 gaatatcact gcacagatac ttctatgagt atatctttat gagagatccg tctacaggat
      181 tgggttataa ggtgtattta caaggtataa tcacaggatc atttacatga ccatacttct
      241 gtaaataata ctaattaacc ttaattattt ttttatggta catgaactac ctgtagctgt
      301 aacgataaac aaatcaccca aaatgaaaaa tactggttat cacagttcag agaactcgta
      361 tgagtgtccc cctgagcaat gcttcatttt ctgtgattct gtgtgataga gttatactga
      421 gtacagtggc agtaacactg ctttccatca cttgaggata aaattcatta aaaaattaat
      481 tgcatgaatt aacaggtttt tttatgaaat gttatgcatc tcaaccaata tgattaacag
      541 aaaacttcat catgacaaca cgtataacgt tcagtatgac cagtgatgag acgctgcgaa
      601 ttgtggatga gtactgtcat acccacaaac tttcgcgctc taaggtaatc aacgcattac
      661 tcagcgccac agcacctgtc cttaatgata ttaactgtta ttatcaactt gcaggcgaac
      721 tgcagtcacg cttattaaat ggagtgtatc aacgggatct tcctcgcaaa cgcaatgtcg
      781 tcagtgcaga gaagtattgt ctggaaatct gggaaaacaa acttttcacc agaagaatcc
      841 tggagtttga cagttcaaat ggtgttctgt acgcgctgaa acataaaaga cactatcgcc
      901 gggataagat gattggaagg gttgaaagtc ggtgtatcaa agacatttgc gaataccaga
      961 tgcagctatc cggggagaag acgaaatatg cctgttttat ttatatcgaa agaacaatat
     1021 acaatcacga taatccatct gataaaatac ctgtaaaagc ggctgttggt aatgcagtca
     1081 tcctgctggc aaaggatgtg atatataacg agtacttttt tgatttgcgg cagtcatttt
     1141 ttgtgagcgt tacagatctg atggtctctg gcgcaaaagg tattccggag acacagacat
     1201 atccggacgt ttattgctgg atccctcttt tttccataaa ttccggcgtg ctcattaccc
     1261 ctgtatataa aattgaccct ctgaaacccg taacggtaaa aaaaccggat aagatcactg
     1321 ttgtttgtaa ttacagagaa taatttcagc cttctacctt caccgcgaca gtgtttacct
     1381 ctgctttatc ttccgttcag taaatacaaa ccaccgctct ggcaggtaca gggcgtggtt
     1441 tatctccgat actcccctgt gcagtaataa tcatcactga aaatactgat cccgtcacgg
     1501 caaaacgcca tcccatattc cggcaacaca aaatcatctt ccgtttatac cggattcact
     1561 gacaaaacag acaacctgta ttgcctgtac acaacagttt ctctcctggc aggaatattc
     1621 ccctactcat tttttacgtt tctccccggt tgttatgccg gtgaaggtgg tgcgtcgttt
     1681 tcatcaccaa cgccggttga gattaacaac atccggagga acattctcat gaatacccca
     1741 gtttcactga tggatgacca gatggtcgac atgtcgttta tcactcagct gaccggcctg
     1801 accgataagt ggttttataa actcatcaag catggagcct ttccgccccc catcaaactg
     1861 ggccgtagct cccgctggct gaaaagcgaa gtggaagcct ggctgcaggc gcgtattgca
     1921 cagtcccgtc cgtaattcct gaccgttatc cgttccaccg cagcaaaggc atgatccggc
     1981 ccccggaggc attttgctgc gcgctaaagg agagaatatt atgcaacaac ttgaaaacag
     2041 gtgtgacctg ctgctcatcc agcatcagaa atggatgaca agcgtgacga ggcttatcgt
     2101 cgctcatgga atggggtctc cgcatcttca tggttatcac cgtctgaccc tggcacactt
     2161 ttttctgccg gaaaaaggct ccgtcatttc ggtggccccc cagggactct atcaggtcgt
     2221 caacccggga acaccaccgt tcattcctgc cattcaggaa gggttaatga ccagcattca
     2281 gacccatgaa atcatgctgc ttacacattt taatctgggc ggcgttttac tcagtgagct
     2341 acatcgtctg ggtgaaaacc ggctggcaaa caggctgaac agcctgctcc ggcgtttcga
     2401 cgatcgtgat ctctaccaca cgctgatatg gctgtgctgg tatgatctga tgtgtgccca
     2461 ctccatgcag ccctggactg aagagttaaa gcataaatca catgcagagc ttgaaaactg
     2521 ggctgtggcg cgcaaacggg aaaagcgcga gctggaactg atgattgacg agtacctgtt
     2581 atacgcctgc tgattcataa cgttcccggg gttccttcct catcgttaca cacagccgga
     2641 cggctatcta tggctgtccg ccaaatgacc atcgccgcat ttcgttccgc tgaaaacggt
     2701 acgtagtcag tgataccgga atttccggag agggccgcag gtatattgct tctgtgcaat
     2761 tgatgcttat ttacattgga tgtagttatt tctgcatcca atgctatcgg aaagagatta
     2821 accagcaaat ggttcctcaa atcgtattga tatttacccg gcgtattgag caccaccatc
     2881 tgacactccg ttcccgcatc aaacgactgg accgaaaaac gatttacttt tcacgtgctg
     2941 tggaaattca cgaaaaaatt attgggtcat tcatcgaaaa acatatgttc tgatgactgg
     3001 agtcatcctc cagaaatata cgtcaactga acaatatcga ttttttatgg gattctatac
     3061 acttttccat atccctctga aaatcagtca tcagctcacc aacacctctt ctgaaacttg
     3121 tattaaactt attgcttaaa tagatgatat aacatctctt ccattgcact gaatcggcga
     3181 aaactgccgt ttcaataatc tcaacaatat tatttatcgg ctaactttat cagtaaacaa
     3241 tggaatgtca cactgatgaa atgaacattc agagtaagat gataaaattc atataaacgc
     3301 cattgctgaa tattatttat ttcatacaga taaacgataa aatacaaaag ggtatatttc
     3361 tcaatgtaac agtcaattat tcatctccac tggcgagcac ggtcagcaca caaacaaagt
     3421 tagtcctcct gcttaatcat tgcgtatcag aaactaaaaa agaaaaaatt ctgcagacaa
     3481 cttaacaatc ttcatcacat ttcctgaaga cagacatatc cacaaacggt agtcagcgtt
     3541 gaattaacaa gtcttacaga gctatacgta gcttataaag ccactcaatg ttcatcatca
     3601 cccgactact tatctggaat accaccagta taaactcatc aataaaaaca cctgttgtta
     3661 aaataagaat actgtcagca gtaaattatt aaaacaacat ctgtccacac tatctgttct
     3721 tttaacgcca acggcagcga aaatagtgca tcacagcctt cttttttctt tcgccaccac
     3781 tcagacaaac acaacactca cacccacacc acaaaacaga atcaaaacat aaaattaaca
     3841 ataacagtca aaaggaaaac acagaatatt gaggtggact gtcaccagga cagcgcatac
     3901 gacgcatgtg atcagcacat cccgttcttc gctggcatcc ccaaaatgca gaacaaacaa
     3961 tcataacatt atgaatataa aagaaaatat gatttttcac catgaaaaaa acatcaatgt
     4021 atctctgtat aaaaataatg ataacatgac ataaacattt aaaaacataa ataaacagca
     4081 acaaaaacat ttgatgatca cttagtgatt aaatacaatt aagacgattt tgttataaga
     4141 gaggcaccat ggagtgagat aatgaataac gaacagatgc ttatcaaatg cagagatccc
     4201 cgtaaatgtc ggttttatgc cgaatatctg aatcacaaat accctaatga attccctcac
     4261 tttgcgcctg acaacccaca aaattgtatc aggaatgtta atttattttt aaaggaaaag
     4321 tattgtaatg tttcacctcc cccctttggt acttttggta ctactgaaga agaagcactc
     4381 aataacgaaa ttaatgagct tgaaattatt gccgggcagg caatactgcc tgaagagagt
     4441 tttctctggt tgaaaactga cgaacttgcg actttcttta cctggactac tatttatctg
     4501 tcgaaggaag accatacaaa attcccgaaa ataaaccata attcatcagg aataaaaaac
     4561 ggaattgaag tcaggaaaat acgaacagtt accgatgaaa cgttaaacac ttacaaaaaa
     4621 agtaaccttc cggataatcc atcatcccat aacgaacgaa taaaagtaat catttcatat
     4681 tttgactcct gccattcttt aattaataaa aatgcaaaaa gcatatacct cgaggaactc
     4741 aggcaggcgt ggcttaaaaa gaaaagcgct tgtcaaaagt ttaagtggct ccttcctgac
     4801 gatgatgaaa tgtgtcagtg gttctggaca agactgaaac aaaaacagga tgaaaaacat
     4861 ccagtacata acccagtgac ccggtggttc ataccatctt caacgaaaga atattatctt
     4921 gcctctcttg tcgcctttgc tttatggaaa gaagcgcctg ataccattga actgttcaga
     4981 acacgaataa atcatgcctg gcatcagaaa aaacaaaagg acaaatatcg gagtgatggt
     5041 aaaaaggcca ttaatataca tataagaagc aatgtgaaag aaatgcttga tgagctcaga
     5101 actgtttacg gtctgagaac aggagagttt tttgaaatgc tgattacaga gaaatatctt
     5161 gaacacaagc gaaggaagta attcattacc tgttacattc taaataatct cagacagata
     5221 ttatctccgt gaatcccccg ccacctttcc ggtgcgcggg gttttgtctt ttttcaccgg
     5281 gaatacatgt atgaatccgt ctgatgccat tgaggcaatt gaaaaaccgc tctcctccct
     5341 gccttactca ctttcccgtc acatcctgga acatctgcgc aaactcacca gtcacgaacc
     5401 cgtgattggc attatgggta aaagcggggc cggtaaatcc tcactctgta atgcactgtt
     5461 tcagggggag gtcaccccgg tcagtgatgt tcacgccggc acccgggaag tgcagcgttt
     5521 ccgtctgagt ggccatggtc acagcatggt tatcactgac ctgcccgggg tgggcgagag
     5581 ccgggacaga gatgcagagt atgaagccct gtaccgtgac attctgcctg aacttgacct
     5641 ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct gtggatgagt atttctggcg
     5701 acacatcctg caccggggac atcagcaggt gctgtttgtg gtgacgcagg ccgacaaaac
     5761 ggagccctgc catgaatggg atatggccgg tattcagcct tctcccgcac aggcacagaa
     5821 tattcgcgaa aaaacggagg cggtattccg tctgttccgg cccgtacatc cggttgttgc
     5881 cgtatcggcc cgcaccggct gggaactgga tacgctggtc agtgcgctaa tgacagcact
     5941 tcccgaccat gccgccagtc ccctgatgac ccgcctgcag gacgggctgc gcacggaatc
     6001 tgtccggggt caggcccgtg aacagtttac cggtgcggtg gaccggatat ttgacacggc
     6061 ggagagcgtc tgtgttgcct ctgttgcccg taaggttctg cgcgctgttc gtgacaccgt
     6121 ggtctctgtt gcccgcgcgg tatggaactg gattttcttc tgaacccggt ggtgatgcac
     6181 cagactcttg ttacagattt ttaaccccat gaaaacacaa tgcaaacaca gccatacccg
     6241 ggttgtcccc ttgcgttgtg tttctcaagt taatagaatg catcgatcat tactatcata
     6301 tcgatcgcta taaacgatcg tttgtggtaa atgacctcct ccgtggttgt catgaaaatg
     6361 cgtctcagga aggttaaata atgcacgaaa agaacatcgc cctgctttgt gatgaagccg
     6421 accgactttt gcaactgaac attaatctgc tccggcaaat ggttgatgaa ccggatgtgt
     6481 tatctgacag taagaacgaa aacagactgc tttttgataa acagaaagca ctgaaaagaa
     6541 ttgaggagct ggagggcgaa caaatcaaaa ccgcccgcag ggagatggtg ctggctgttg
     6601 tcggcacgat gaaagcaggt aaatcaacta ccatcaacgc cattgtgggg caggaaattc
     6661 tgcctaaccg taaccgcccc atgacctctg taccgacgct catccgccac gttcccggaa
     6721 aaactgagcc ggtcctccat ctggaacata ttcagcccgt ccgcaattta ttaagcacac
     6781 tgcaacaaaa actcgacacc ccggcaggac agcaagtcgc acagaccctg cagcaaaccg
     6841 gggatacctg cgaactgctg gatattctgg caaatgatgg ctggctcaaa aatgaatacc
     6901 acggggaaga cgaaatcttt accggactgg catcgttaaa cgatctggtt cgccttgctg
     6961 cggcaatggg gagtgagttt ccttttgatg aatacgcaga agtgcagaaa ctgccggtga
     7021 tcgacgtgga attcagccat ctggtgggga tggatgcatg ccagggaaca ctcacactgc
     7081 tggatacccc cggccctaat gaggccggac aaccgcagat ggaagtgatg atgcgggatc
     7141 aactgcagaa agcctctgcg gtgctggcgg tgatggatta cacccagatg aactcaaaag
     7201 cggatgaaga cgtccgtaaa gagcttaatg ccattgctga cgtatcagcc ggccgcctgt
     7261 ttgtgctggt caataaattt gatgagaaag accgcaatgg cgatggggca gatgccgtac
     7321 gccagaaagt tccggcaatg ctgaacagcg atgtgctgcc cgcctcccgc gtttatccag
     7381 gctcatcacg tcaggcgtac ctggctaacc gtgcgcttca tgagttacgg aaaaacagaa
     7441 cccttcctgt tgatgaagcc tgggtcgatg attttgtcag ggaagccttc ggtcgcatga
     7501 aaaaagaata cgtctgtaaa gacagtgaaa tggcaaccga aggggcgaca gacctgtggg
     7561 aaggctcact tatcgatcaa ctgataacgg aagtcataca gagctcccat tccagagcgg
     7621 cggcactggc ggttgattct gccgccgcaa aactgatgca gaatgcggaa aatgtcagcg
     7681 aatatctgtc attacgccat caggggctac agcaaagcat tcagtcactg cagtcgcata
     7741 tcaccagcct gcttgcggat atccgggaaa tcgcggactg tcaggagcag gtgaccgctg
     7801 atgtcagaat ggccatggag gagatcgatg ccagaacccg ggaattactg acgggggtct
     7861 gcacctcact ggaagaggag ctgaatgact atttcagaag cggtaaacgc aaagaacagc
     7921 aaatgctgga ggaagaaaat tcagcgcagc cccgggagag aaacgcattc gctttctttc
     7981 acgatatttt cggaaccgga aatcagcatg acaggatgcg ggactttgat ccggacagcc
     8041 ccgaaataaa attcagtgat cgcagggaag cccttgaact gatgacgcaa atcgaatcga
     8101 ccgtgaccag cctgcaccgt gaggctgaag cacagttccg tcctgaactg gaaaaaatcg
     8161 tcaggggtat tgaaacaggt tttcgtggta cggccctgta cgccacagaa aatattgccg
     8221 gacgcatcaa tgcccgtctg gaggatgaag gtttcactgt aaaaatcagt tttccggcag
     8281 tcagccagtt acagacccgg ctcgcagtaa aaacaaatct gagcgcgctt atggaggaaa
     8341 gaacggagac agtaacccgt cgctgtcgac aggatggtgt ctggggtacg ctttgtcgat
     8401 gggcaaacac cagtgactgg ggctggaaag agtacagtgt ggatgtgagc cgcagcgtga
     8461 tcaatatcaa caaggtcaga gaggaagtga tgtcactgac ccgggcatat ttcggggagc
     8521 tgcaggcatc cattgagcag gatattaacc agcccgtccg ccaggaaatc gatgcctttt
     8581 tctgtacatt cagggagaaa gttgaacagc tgcgtaacac gctgattcag agctctgaag
     8641 atcataaacg cgatcagcag gcgcaggaac ggctgaccgg gcgtcttcag gcgttaaatg
     8701 aaagggttcc tgagctcatt actgacagta aggcgttaag ggaagaactg gagacaatgc
     8761 tgtgacttca ccatttattc aacaaattgc tgataaccgg gtatgtcagg tactcacctg
     8821 tcttcctgaa aaatttgtgg ttgatttcgc aaacggtatt gatgttgcac aggagcatat
     8881 ccgtacggcc ggagagcgca cgttcttccg gcggttaaaa gaggggctga gcggcgaagg
     8941 tactgcacgg cagaatgcca tcaatgcttc gcttgcacag ggcgttgagg cgtccctgcg
     9001 ctggctgacg gagctgacaa cgtcacttgc caccacaaac tacgcgatta cccgggtaaa
     9061 cgacagggtc agttcactgg tcagtgatac tgccaggctg gcgcattatt ccgcagatac
     9121 gcgggagcag ttactcaccc tggccgatca ggttcaccag aaactgaatc atcttgaaga
     9181 aaaactccac cgtgttgacc tggttcagcg ggcgcagtta catcttgagc agatattctc
     9241 ctggtggagc gccgggcgat atgcgtcatt ttcccctgcc ggacgttgtt atgtggcact
     9301 tgaa
//
LOCUS       DAJTMX010000145         9291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTMX010000145 DAJTMX010000000
VERSION     DAJTMX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.145"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO145_003513"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO145_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5468504.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO145_003514"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO145_003514"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5468505.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO145_003515"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO145_003515"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5468506.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO145_003516"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO145_003516"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5468507.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO145_003517"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO145_003517"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5468508.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO145_003518"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO145_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5468509.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO145_003519"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO145_003519"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5468510.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO145_003520"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO145_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5468511.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO145_003521"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO145_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5468512.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO145_003522"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO145_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5468513.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2357 g         2224 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTMX010000146         9286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTMX010000146 DAJTMX010000000
VERSION     DAJTMX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.146"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="OO145_003523"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="OO145_003523"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5468514.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO145_003524"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO145_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5468515.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO145_003525"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO145_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5468516.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="OO145_003526"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="OO145_003526"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5468517.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="OO145_003527"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="OO145_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="OO145_003528"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="OO145_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5468518.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="OO145_003529"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="OO145_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5468519.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7297..7566)
                     /locus_tag="OO145_003530"
     CDS             complement(7297..7566)
                     /locus_tag="OO145_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5468520.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7623..8291
                     /locus_tag="OO145_003531"
     CDS             7623..8291
                     /locus_tag="OO145_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5468521.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8607..>9286
                     /locus_tag="OO145_003532"
     CDS             8607..>9286
                     /locus_tag="OO145_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5468522.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT         2667 a         2086 c         1897 g         2636 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatat aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
     7261 cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg
     7321 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt
     7381 ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg
     7441 aagtggcgca atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca
     7501 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
     7561 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
     7621 atatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
     7681 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
     7741 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
     7801 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
     7861 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
     7921 aacataaaat tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg
     7981 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
     8041 gtgtaatacg caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa
     8101 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
     8161 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     8221 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     8281 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta
     8341 cgatttgggg ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat
     8401 gaacgtacga tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat
     8461 ctaagaaaat agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac
     8521 aataaatttc aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta
     8581 tcaggatatc cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg
     8641 gaaacgtctc tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg
     8701 aatgggttga atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc
     8761 ggctccaatt atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca
     8821 gatctctacc acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat
     8881 caatgctccc cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat
     8941 ctgcacttcc tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt
     9001 tcgcacttcc tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt
     9061 ctgtacttcc gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg
     9121 aagtaatacc agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc
     9181 agaaccggat cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca
     9241 atcttagaga aaatcaacta tctcccagaa taatgcaaac tttgtt
//
LOCUS       DAJTMX010000147         9241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTMX010000147 DAJTMX010000000
VERSION     DAJTMX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.147"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO145_003533"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO145_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5468523.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO145_003534"
     CDS             1828..3147
                     /locus_tag="OO145_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5468524.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO145_003535"
     CDS             3445..3486
                     /locus_tag="OO145_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468525.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO145_003536"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO145_003536"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5468526.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO145_003537"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO145_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO145_003538"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO145_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO145_003539"
     CDS             5815..6711
                     /locus_tag="OO145_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5468527.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO145_003540"
     CDS             6708..7682
                     /locus_tag="OO145_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5468528.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO145_003541"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO145_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO145_003542"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO145_003542"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5468529.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2316 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 a
//
LOCUS       DAJTMX010000148         9191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTMX010000148 DAJTMX010000000
VERSION     DAJTMX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.148"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..904)
                     /locus_tag="OO145_003543"
     CDS             complement(<1..904)
                     /locus_tag="OO145_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5468530.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO145_003544"
     CDS             964..1242
                     /locus_tag="OO145_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468531.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO145_003545"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO145_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5468532.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO145_003546"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO145_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5468533.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO145_003547"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO145_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5468534.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO145_003548"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO145_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5468535.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="OO145_003549"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="OO145_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="OO145_003550"
     CDS             complement(4922..5452)
                     /locus_tag="OO145_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5468536.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO145_003551"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO145_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5468537.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="OO145_003552"
     CDS             complement(6421..7644)
                     /locus_tag="OO145_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468538.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9191)
                     /locus_tag="OO145_003553"
     CDS             complement(8225..>9191)
                     /locus_tag="OO145_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5468539.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2681 a         1827 c         1922 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc t
//
LOCUS       DAJTMX010000149         9070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTMX010000149 DAJTMX010000000
VERSION     DAJTMX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.149"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..923
                     /locus_tag="OO145_003554"
     CDS             <1..923
                     /locus_tag="OO145_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5468540.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO145_003555"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO145_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5468541.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO145_003556"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO145_003556"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5468542.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO145_003557"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO145_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5468543.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO145_003558"
     CDS             4446..4805
                     /locus_tag="OO145_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5468544.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO145_003559"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO145_003559"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5468545.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="OO145_003560"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="OO145_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5468546.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="OO145_003561"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="OO145_003561"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5468547.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2066 a         2196 c         2448 g         2360 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg
//
LOCUS       DAJTMX010000150         9038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTMX010000150 DAJTMX010000000
VERSION     DAJTMX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.150"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1046)
                     /gene="murD"
                     /locus_tag="OO145_003562"
     CDS             complement(<1..1046)
                     /gene="murD"
                     /locus_tag="OO145_003562"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5468548.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDG"
     gene            complement(1049..2131)
                     /gene="mraY"
                     /locus_tag="OO145_003563"
     CDS             complement(1049..2131)
                     /gene="mraY"
                     /locus_tag="OO145_003563"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5468549.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(2125..3483)
                     /gene="murF"
                     /locus_tag="OO145_003564"
     CDS             complement(2125..3483)
                     /gene="murF"
                     /locus_tag="OO145_003564"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5468550.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(3480..4967)
                     /gene="murE"
                     /locus_tag="OO145_003565"
     CDS             complement(3480..4967)
                     /gene="murE"
                     /locus_tag="OO145_003565"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5468551.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(4954..6720)
                     /gene="ftsI"
                     /locus_tag="OO145_003566"
     CDS             complement(4954..6720)
                     /gene="ftsI"
                     /locus_tag="OO145_003566"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5468552.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(6736..7101)
                     /gene="ftsL"
                     /locus_tag="OO145_003567"
     CDS             complement(6736..7101)
                     /gene="ftsL"
                     /locus_tag="OO145_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5468553.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(7098..8039)
                     /gene="rsmH"
                     /locus_tag="OO145_003568"
     CDS             complement(7098..8039)
                     /gene="rsmH"
                     /locus_tag="OO145_003568"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5468554.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(8041..8499)
                     /gene="mraZ"
                     /locus_tag="OO145_003569"
     CDS             complement(8041..8499)
                     /gene="mraZ"
                     /locus_tag="OO145_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5468555.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         2121 a         2612 c         2241 g         2064 t
ORIGIN      
        1 ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta cgtgcaggcc attcagcgcc
       61 gcttccgtac tgccgacgtt ggtcgctttt gaatcgttaa tccagcgcac accgttatgc
      121 tccagcacca cttcaaagcg atgcggcaga ccggtgaagg tggttaacgc tttcaggctg
      181 ctggcgcgcg gtaaccctgc ggcatctgcc agcgccagcg ccaccagcgc attggtgtag
      241 ttatgctgcc cggaaagttt catctctttc acattcagca ctttctcgcc tttaacccgc
      301 agccaggttt cgccctgctg atgattcagg tgatagtcac ccatgttgac gccaaagctg
      361 acgcagcgtt catccgcacc gcgaatcggc attgttaagg catcatcagc attaaccacg
      421 caaactttcg cgttttcgta aatgcgcagt tttgctgcac gatactgttg taaaccaaac
      481 gggtagcgat ccatatgatc ttcagtcacg ttcagaatgg tcgctgctac cgcctgtaag
      541 ctggaggtgg tttccagctg gaagctcgac agttccagca cgtacagttc acactcatca
      601 tccagtagca tcaacgcagg caggccaata ttgccaccca caccaacgtt aacccccgcc
      661 gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc
      721 gccacaatcg gtgcttgtgc ttcgcgacaa aacagctcga tatcgccaac gatttcgatt
      781 ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca
      841 atcagatctg ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct
      901 tcgggtaatt tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca
      961 ccgcgagcga ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc gataatgaca
     1021 acatttttac cctggtaatc agccatgatt aacgtacctt cagcgttgcc agaccaatca
     1081 ggaccagcat cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt
     1141 tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa
     1201 aggagccgac ctgcaggatg acagaaagcg tttctaccac gaacacgccc cccataatca
     1261 ccagcaggaa ttcctgacgt agcagtacgg cgataatgcc taacgcacca cctaacgcca
     1321 gcgaaccgac atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca
     1381 gtcctgcccc gactatcgcg gtacagacaa taaccagttc cccggcgtgt cgcagatacg
     1441 gtatgtgtaa atagctggca aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac
     1501 caccggcgac aaatacggtc ggcataattg ccagaccgtc aagaccatcg gtcaggttta
     1561 ccgcgttgcc agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagtt
     1621 gcggcatcac atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg
     1681 caaggtacaa agcgaaagca acacccagcg caatgaccga catccagaaa tatttccaac
     1741 gagcgatcaa ccctttggtg tctttacgca ccactttgcg ataatcatca acaaagccaa
     1801 taacaccgta acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag
     1861 cccacagcag cacggagatc acaatcgctg tcaggatcat aatcccgccc atggtcggcg
     1921 taccgcgctt gctgaagtgt gattcaggac cgtcgttacg cactacctga ccaaaggaaa
     1981 gtttttgcaa atgagcaatc atgcgcgggc ccatccacaa tgagatgaac agcgcggtca
     2041 gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt
     2101 tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaaag cgcgtactac
     2161 ctcttccatg gcagcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc
     2221 aatcagtgat ttaagacgcg taattaacgc agttttatca gcaaaatgtt cgccaacgcc
     2281 gctggcggtg ctgatagcat ggctttgttt acccatgctt aacacgcggt caataccagc
     2341 agcttttgcc gcctcaccca cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc
     2401 catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct gaacagctgc
     2461 ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag
     2521 ttggatgggg aacagacggc caggaacagc tttcagattt gccagccccg ctttgatagc
     2581 atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt
     2641 gtgacgcccc ggcaacggca gcagaacatc gacgctaccg gttggggttt gtagggtaaa
     2701 ttccataccg tgcgaggtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt
     2761 gggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt
     2821 gttgtcggcg ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt
     2881 cgctttcgcg acacccgcaa gcgagccaaa accttccaga tgcgccgctg ccaggttgtt
     2941 gaccagcgcc gcttccgggc gagtcagact cacagtccag gcaatctcgc cctgatggtt
     3001 cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg
     3061 tacaccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa
     3121 aatcgccgcc gtcatctctt taacggaggt tttgccggag gaccccgtca gagcaaccac
     3181 gagcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc
     3241 cttgacgatt aactgcggca ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc
     3301 gccgccagct ttcgcctggt cggcaaaatc gtgggcatca aaacgttcac ctttcagggc
     3361 aacaaacagg cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat
     3421 atctgcacct tgcagttcac cgttgagaat atcggtaagt tggctaaggg ttacgctaat
     3481 catgcaatcc cccccagcag acgcgccacc gtgacgcgat cggagtagtc cagacgctga
     3541 ttgccaacaa tctggtaatc ctcatggcct ttgcccgcga ccagtaccac atcattctct
     3601 ttagcctgca taacggcgca agtcaccgct tcagcacggc cttccatcac tttggcatgt
     3661 ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc
     3721 gggttatcgt ccgtcaccac cgccacgtca gcaaactctt cggcaattgc gcccatcagt
     3781 ggacgcttac ctttatcgcg atcgccacca cagccaaaga cgcaccacag cttgcccgca
     3841 cagtgcaggc gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc
     3901 accaccaccg tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc
     3961 agacgcgcgg cggttttcag cagatcagcc agtggatagc ccagcgccag cagtgttgcc
     4021 agcgcgagca acaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca
     4081 tcgccccagc ttgaactaaa gcgaatggtc gcgccgctgt cgtgatagtt cacttcggtc
     4141 gctttcaacc agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaacc
     4201 gcgtccggca gttttgccag ccagcggcgg cccacttcat cgtcggcgtt aataatcgcc
     4261 tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca
     4321 ccatgataat caaggtgatc gcggcttaag ttggtaaaga ccgacgccgg aaatttcaat
     4381 gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaaagtcgcg
     4441 ccctgatcca ccagccccgc cagctcatgc tgaacatcaa ctgccgaacc ggtagtattt
     4501 tctgtcggga tcactttccc cagcagaccg ttaccaacgg tgcccattac cgcgctggtt
     4561 tcgccaagca gttggctcca ctgcgccaga agctgggtcg tcgtggtttt gccgttggtg
     4621 cccgttacgc ctacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt
     4681 gcagataaac gctcgttgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg
     4741 atttcaccgt cggtcgcctc atctttcgcc tctgcaataa tggcagccac accttgcgct
     4801 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
     4861 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
     4921 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
     4981 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
     5041 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
     5101 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
     5161 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
     5221 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
     5281 gccgccgcct ggtagcgcca cgctttccat catatgcacc acggtgcgaa caatggattc
     5341 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
     5401 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
     5461 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
     5521 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
     5581 gtaagtatct actaacgctg aggacggcat cgttaacgcc agcttggaaa caccgacgtt
     5641 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
     5701 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
     5761 acgttgcaac gcggtcatca ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
     5821 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
     5881 tgacgggctg ttagccatcg ccagcacttc acctgtgttg acatccacca gcacggcgct
     5941 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
     6001 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
     6061 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
     6121 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
     6181 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
     6241 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagagt ttacctgacg
     6301 cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag
     6361 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
     6421 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
     6481 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
     6541 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
     6601 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
     6661 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
     6721 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
     6781 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
     6841 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
     6901 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa
     6961 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
     7021 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
     7081 ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac tgaactacgg
     7141 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
     7201 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
     7261 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
     7321 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
     7381 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
     7441 ggatgtttaa acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg
     7501 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
     7561 aaatgctctt caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt
     7621 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
     7681 cgcataaagg aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac gccaagatcg
     7741 aggagaatgc cgtcgatctt gccaataaga tcgcgctcgg caacgtattc gcccagcgcg
     7801 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
     7861 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
     7921 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
     7981 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
     8041 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
     8101 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
     8161 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
     8221 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
     8281 cacacggcgc tcaaccgggt tcatgctcaa cagacgcgat aatttttgct cgataatttc
     8341 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
     8401 ttgaccggca gcgttctcaa gcagctgttc ccgataacgg gtaggcactg ataagcgccc
     8461 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
     8521 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
     8581 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
     8641 ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa gaaaaaatcc
     8701 ctcatcgtca tcactgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
     8761 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cttgttacgt
     8821 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgt ttgttaaatc
     8881 ggcaattcat cgccgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
     8941 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaaccat ttatcaataa
     9001 tagtcaggaa tagtcttatt tactttaagg atattgat
//
LOCUS       DAJTMX010000151         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTMX010000151 DAJTMX010000000
VERSION     DAJTMX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.151"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO145_003570"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO145_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5468556.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO145_003571"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO145_003571"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5468557.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO145_003572"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO145_003572"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5468558.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO145_003573"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO145_003573"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5468559.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="OO145_003574"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="OO145_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5468560.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="OO145_003575"
     CDS             5562..6035
                     /locus_tag="OO145_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5468561.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="OO145_003576"
     CDS             6064..6606
                     /locus_tag="OO145_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5468562.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="OO145_003577"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="OO145_003577"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5468563.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="OO145_003578"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="OO145_003578"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5468564.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="OO145_003579"
     CDS             8003..8260
                     /locus_tag="OO145_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5468565.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="OO145_003580"
     CDS             complement(8236..8790)
                     /locus_tag="OO145_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5468566.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2191 c         2253 g         2327 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgta gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAJTMX010000152         8993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTMX010000152 DAJTMX010000000
VERSION     DAJTMX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.152"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO145_003581"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO145_003581"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5468567.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO145_003582"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO145_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5468568.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="OO145_003583"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="OO145_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5468569.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO145_003584"
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO145_003584"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5468570.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO145_003585"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO145_003585"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5468571.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="OO145_003586"
     CDS             complement(7822..8133)
                     /locus_tag="OO145_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5468572.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO145_003587"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO145_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5468573.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="OO145_003588"
     CDS             complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="OO145_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5468574.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2250 a         2328 c         2133 g         2282 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtgat
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ttgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctaa ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacatc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtggct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgc aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacta taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc cccaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaagg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaag attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tta
//
LOCUS       DAJTMX010000153         8957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTMX010000153 DAJTMX010000000
VERSION     DAJTMX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.153"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..2341)
                     /locus_tag="OO145_003589"
     CDS             complement(<1..2341)
                     /locus_tag="OO145_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5468575.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="OO145_003590"
     CDS             complement(2275..2898)
                     /locus_tag="OO145_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5468576.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="OO145_003591"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="OO145_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO145_003592"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO145_003592"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5468577.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="OO145_003593"
     CDS             7506..8228
                     /locus_tag="OO145_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5468578.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..>8957)
                     /locus_tag="OO145_003594"
     CDS             complement(8356..>8957)
                     /locus_tag="OO145_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5468579.1"
                     /translation="TRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVS
                     EHEDGVDRYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQ
                     SQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIK
                     VGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2061 a         2462 c         2263 g         2171 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttc
//
LOCUS       DAJTMX010000154         8757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTMX010000154 DAJTMX010000000
VERSION     DAJTMX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.154"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            5..436
                     /locus_tag="OO145_003595"
     CDS             5..436
                     /locus_tag="OO145_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5468580.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO145_003596"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO145_003596"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5468581.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO145_003597"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO145_003597"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5468582.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO145_003598"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO145_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5468583.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO145_003599"
     CDS             complement(4438..5145)
                     /locus_tag="OO145_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5468584.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO145_003600"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO145_003600"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5468585.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO145_003601"
     CDS             complement(6003..6206)
                     /locus_tag="OO145_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5468586.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO145_003602"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO145_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8757
                     /locus_tag="OO145_003603"
     CDS             7870..>8757
                     /locus_tag="OO145_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5468587.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2031 a         2387 c         2250 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacct
//
LOCUS       DAJTMX010000155         8731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTMX010000155 DAJTMX010000000
VERSION     DAJTMX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.155"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO145_003604"
     CDS             complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO145_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5468588.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1033..2124
                     /gene="rutA"
                     /locus_tag="OO145_003605"
     CDS             1033..2124
                     /gene="rutA"
                     /locus_tag="OO145_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5468589.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2124..2816
                     /gene="rutB"
                     /locus_tag="OO145_003606"
     CDS             2124..2816
                     /gene="rutB"
                     /locus_tag="OO145_003606"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5468590.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2828..3214
                     /gene="rutC"
                     /locus_tag="OO145_003607"
     CDS             2828..3214
                     /gene="rutC"
                     /locus_tag="OO145_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5468591.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3222..4022
                     /gene="rutD"
                     /locus_tag="OO145_003608"
     CDS             3222..4022
                     /gene="rutD"
                     /locus_tag="OO145_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5468592.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4032..4622
                     /locus_tag="OO145_003609"
     CDS             4032..4622
                     /locus_tag="OO145_003609"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5468593.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4633..5127
                     /gene="rutF"
                     /locus_tag="OO145_003610"
     CDS             4633..5127
                     /gene="rutF"
                     /locus_tag="OO145_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5468594.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5148..6476
                     /gene="rutG"
                     /locus_tag="OO145_003611"
     CDS             5148..6476
                     /gene="rutG"
                     /locus_tag="OO145_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5468595.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6558..6665)
                     /locus_tag="OO145_003612"
     CDS             complement(6558..6665)
                     /locus_tag="OO145_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468596.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7038..7634
                     /gene="wrbA"
                     /locus_tag="OO145_003613"
     CDS             7038..7634
                     /gene="wrbA"
                     /locus_tag="OO145_003613"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5468597.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7655..7882
                     /locus_tag="OO145_003614"
     CDS             7655..7882
                     /locus_tag="OO145_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5468598.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7920..>8731)
                     /gene="agp"
                     /locus_tag="OO145_003615"
     CDS             complement(7920..>8731)
                     /gene="agp"
                     /locus_tag="OO145_003615"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5468599.1"
                     /translation="MDPTFNPMITDDSAAFSEQAVAAMEKELSKLQLTDSYQLLEKIV
                     NYKDSPACKEKQQCSLVDGKNTFSAKYQQEPGVSGPLKVGNSLVDAFTLQYYEGFPMD
                     QVAWGEIKSDQQWKVLSKLKNGYQDSLFTSPEVARNVAKPLVSYIDKALVTDRASAPK
                     ITVLVGHDSNIASLLTALDFKPYQLHDQNERTPIGGKIVFQRWHDSKANRDLMKIEYV
                     YQSAEQLRNADALTLQAPAQRVTLELSGCPIDANGFCPMDKFDSVLNEAVK"
BASE COUNT         1943 a         2302 c         2354 g         2132 t
ORIGIN      
        1 tgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
       61 cggttaaaca ggtagccgga ggatgttaca acctcctccg tcatctttaa cgtggtcgaa
      121 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
      181 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
      241 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
      301 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
      361 catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
      421 gataatcgcg tgagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcgggg
      481 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
      541 cggcaatata cagcgcctct tttgacggga agtaatacag cagattggtt tttgaaacac
      601 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
      661 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
      721 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggt aacaggatag
      781 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacggatg catgttttat
      841 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata acacaatgct
      901 aacacattaa taatattcac tttttaaaaa ctggcatccg ctttgcgaac aagccaatac
      961 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
     1021 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
     1081 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgcgcaaaaa
     1141 gcggagcatt accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
     1201 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg tctggcagcc
     1261 gtgacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
     1321 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
     1381 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
     1441 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
     1501 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
     1561 caaccgagta tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
     1621 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac acccacggct
     1681 ttcgccccga ccgctgcgcg gatgaaacag gccacagagc aaaccggacg cgacgttggc
     1741 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccactcg cgccaaatgg
     1801 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
     1861 aaagataccc gctccggtac tgacaccaac gttcgtcaga tggccgatcc cacttcggcg
     1921 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
     1981 gaagtcgcaa gcgtgcctgg tgcagaaggc gtgctgttga ccttcgatga ttttctgtcg
     2041 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctctct
     2101 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
     2161 tcgatccgca gcaaagtgcg cagatcgtgg tggatatgca aaacgcttat gccacgccag
     2221 gcggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
     2281 ttcaaaccgc tgtgaccgca gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg
     2341 gctgggatga acagtatgtc gaggctggcg gccccggctc accgaatttt cataaatcga
     2401 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
     2461 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
     2521 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttacgc agccgtggaa
     2581 tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
     2641 atggcttttt tctggagcat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
     2701 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
     2761 acgtcgaaac gttctgcgac gcgctttctc ccacgtcctt tgctcgtatc gcttaaggag
     2821 tttagcgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
     2881 cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg cttttgatca
     2941 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
     3001 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
     3061 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
     3121 gggtgataaa ccggcgcgat tctacattca gtgcggactg gtaaaacctg acgcgctggt
     3181 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcat
     3241 ctccccctta tgctgatgca cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
     3301 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
     3361 agcgcggcac cggcaataat cccgacacac tggcagaaga ttacagtatc gcccagatgg
     3421 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
     3481 atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga ttatcccgcg tcggtaacta
     3541 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
     3601 ttcgcgaacg attactgtat agcggcggcg cgcaagcatg ggtggaagcg cagccgttgt
     3661 tcctctatcc cgccgactgg atggcggccc gcgcacctcg cctggaggca gaagacgcgc
     3721 tggcactggc gcattttcag ggcaaaaata atttactacg tcgacttaac gccctcaaac
     3781 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
     3841 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
     3901 gccagaaaat ggtgatgccc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
     3961 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
     4021 aaggaattgc tatgaacgaa gccgttagcc caggtgcgct tagcaccctg ttcaccgatg
     4081 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta caggagcttt
     4141 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
     4201 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
     4261 aaaccctgac cgcgcccgtc accgctatcg tcgcctggga cagtgaattt tatgaacggt
     4321 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
     4381 ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc ttatctgatc gtcgcttgcc
     4441 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaacac gttgacgacg
     4501 ccttttttgc gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
     4561 gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
     4621 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
     4681 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
     4741 agcgccgtct gcagcgtgac cgacacgccg cccacattac tggtgtgcct gaatcgtggg
     4801 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
     4861 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
     4921 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
     4981 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
     5041 atcgaagcga ttcatcgtca caccacaccc tacgggctgg tgtggttcga tcgcagttat
     5101 cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
     5161 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
     5221 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
     5281 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
     5341 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
     5401 gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
     5461 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
     5521 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
     5581 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
     5641 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
     5701 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
     5761 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
     5821 ttactctcgt cagctatgct gcctggttcg gtctgccgca tttttccact cctggtttta
     5881 atggccaggc gatgatgttg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
     5941 gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcgag
     6001 cattcgtcgg tgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtca
     6061 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
     6121 ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc gccgaaattt ggcgcgctga
     6181 tccataccat tccggccgcg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
     6241 ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga tttaagccag aacgctaatt
     6301 tgattatggt cgcagtaacc ttcgtgctgg gcgcgggtga ttttgctctc acgcttggcg
     6361 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttat
     6421 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
     6481 aaaaccgccg ggttaccggc ggtgatgact ttttgacgag cttaagaaaa aaatgcattc
     6541 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
     6601 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
     6661 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
     6721 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc ggacagaccc
     6781 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct ccaataatta
     6841 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
     6901 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
     6961 ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
     7021 acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg tacggacata
     7081 ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa agtggatggc gcagaagttg
     7141 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     7201 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     7261 gtacacctac ccgctttggc aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     7321 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     7381 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     7441 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     7501 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggctca cgccagccaa
     7561 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     7621 aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7681 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7741 cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt tgggaagaag gtagtgatga
     7801 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7861 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt ctcgcggggg agttttctgt
     7921 tatttcaccg cctcattcaa cacgctatca aacttatcca tcgggcagaa accgttggca
     7981 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     8041 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     8101 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     8161 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggctatg
     8221 ttggagtcgt gcccaactaa caccgtgatt ttcggagcac tggcgcgatc ggtgaccagg
     8281 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     8341 aacagactgt cctggtaacc gtttttcagc ttcgacaata ccttccactg ctggtcagat
     8401 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     8461 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cacctggttc ttgttgatac
     8521 ttggcgctaa aggtattttt gccatccacc agcgaacact gttgtttctc tttacaggca
     8581 ggggaatctt tatagttaac gattttttcc agtagctggt agctgtcggt aagctggagt
     8641 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8701 gtgatcatcg ggttaaaggt tgggtccata g
//
LOCUS       DAJTMX010000156         8696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTMX010000156 DAJTMX010000000
VERSION     DAJTMX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.156"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1216)
                     /locus_tag="OO145_003616"
     CDS             complement(<1..1216)
                     /locus_tag="OO145_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HCR5468600.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGS"
     gene            complement(1266..1613)
                     /gene="tnpB"
                     /locus_tag="OO145_003617"
     CDS             complement(1266..1613)
                     /gene="tnpB"
                     /locus_tag="OO145_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5468601.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(1610..1999)
                     /locus_tag="OO145_003618"
     CDS             complement(1610..1999)
                     /locus_tag="OO145_003618"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5468602.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(2345..2779)
                     /locus_tag="OO145_003619"
     CDS             complement(2345..2779)
                     /locus_tag="OO145_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5468603.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(2767..3168)
                     /locus_tag="OO145_003620"
     CDS             complement(2767..3168)
                     /locus_tag="OO145_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468604.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(3428..3997)
                     /locus_tag="OO145_003621"
     CDS             complement(3428..3997)
                     /locus_tag="OO145_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5468605.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(4742..4885)
                     /locus_tag="OO145_003622"
                     /pseudo
     CDS             complement(4742..4885)
                     /locus_tag="OO145_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(5219..5485)
                     /locus_tag="OO145_003623"
     CDS             complement(5219..5485)
                     /locus_tag="OO145_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468606.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            5626..5832
                     /locus_tag="OO145_003624"
     CDS             5626..5832
                     /locus_tag="OO145_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5468607.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            5927..6529
                     /locus_tag="OO145_003625"
     CDS             5927..6529
                     /locus_tag="OO145_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468608.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            6700..6897
                     /locus_tag="OO145_003626"
     CDS             6700..6897
                     /locus_tag="OO145_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468609.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            7729..8646
                     /locus_tag="OO145_003627"
     CDS             7729..8646
                     /locus_tag="OO145_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468610.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT         2058 a         2326 c         2141 g         2171 t
ORIGIN      
        1 cagagccgaa gaacagggtc ttcatctttt cacgcaacca gctttccagg gatttcaaca
       61 gcggtttcgt ttttcgctga cgttcagcaa gccgctgctc tgccggcatt ccccttatat
      121 ccgcctctat ggcgtacaac tgaccgatct gctccagggc ttcttccgtc agtgctgacg
      181 ggatgcggac gtgcacatcg tggatctttc ggcgggcatg agcccagcag gcagcttccg
      241 ttatcccacc attgcgatac agctcgttga acccggcgta cgcatccgct tgcagcacac
      301 cgctgaagca agcaagatga gtctgcggat ggatgctttt tctgtccggg ctgtaagcga
      361 accacactgc aggtgccaac gctgacccgg cattgcggtc atcacgaaca tacgcccaca
      421 accgcccggt cttcgtcttc ttattacccg gcagcagtac ctggaccggg gtatcatcgg
      481 catggagttt gccgtcagtc atgacatagc catgaagcgc ctcttccagc ggagacagca
      541 gccggcagca tgcatccacc cagcccgaca gcagtgaacg gctcagctcc acaccttgcc
      601 ggccgtatat ttctgactgg caatacagcg gggtgtgctc tgcatacttc gaggtcagca
      661 cgcgggccag cagccccggt ccggcgatac cccgctcgat gggccgcgaa ggtgcaggtg
      721 cctgcacgat ggtatcgcac tgagtacagg catgtttttc ccgtaccgtc cggataaccc
      781 ggaaggcgct acgcatcaac tccagctgtt cggcggtatc ctcgcccaga tagctcagtg
      841 aaccgccgca gttcgggcag cacggcgccg caggcaacag tcgcttttcg tcacggggta
      901 gtgattcagg gaacggctta cgggtgcggg tctgacgcaa cggacgctgt actgccgggt
      961 catacaccct accagtcagc gtatcgctct ctttctgaag ccggttcaga tcggcttcca
     1021 tttgtgcgat acggcgggag actttttcgg aacgactgcc gaagttcatc cggcggagtt
     1081 tatccagctg cgcctgcaga tggtctattt cgcgctcccg gttgctcagc ttttcctgca
     1141 gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt cagcaggaag atgtcgtcag
     1201 aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa ccaggataaa
     1261 gagatttaca gcatggtcag ggaggtcagc agccgcttgg gctgtcgcca gtcgatacct
     1321 tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg ggctgacggc
     1381 caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc gccggtggac
     1441 cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaatatg gccggacatg
     1501 ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagct atttctcata
     1561 tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat catcgcttca
     1621 gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag cggagcgtcc
     1681 cgtgccggaa cgttacctca cagctgatac tgagggtttc cgggtcctct gcgagcgatt
     1741 ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc tctgaagaag
     1801 gtaatagcag ctttccctcg cgccattgtt gtcgccattt gaacaacaga ttggcgttaa
     1861 tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag gcagcaacga
     1921 gcagctgttt aaattcggga ggataattag ggcagccttt tcgcctgccg ggagtcacat
     1981 ttttctgcat atctgacact ttggttccca ctacttattt ggtggacacc actttgtcta
     2041 attcgtcaga ttctgaccag acggttcagg ctgtacgctt accactattt tatccctgtt
     2101 gaatttggtt aagccggtgc agacgcgccg ggaaacgcac acctgaaccg gcacaataaa
     2161 atacaataca accccctgtt actggagata cgaatggaat gacgaggcag cctgtcagtc
     2221 acagggggct gttggcataa aatcaacgga aaggccacca acaccggaat taagctccac
     2281 agtccccgca ctggcaatcc attcccggga aaggcatcac cgcaaactgc tccggaggca
     2341 accattacag cctctgtaac ggattatcag ccctggcatc ttccgacggc acctcctgcc
     2401 gcccccacgc cctgctgtat accccggtac cgcagcgtgg gcagcacttt tccccctcat
     2461 aatcttcgtc acacatcaca caaaaccact ttctcacctt caccgtataa tccggaaccg
     2521 tcatgccctg cagatgcaaa cgacgggcct ctttctctgc cgggtcaagc caggtacatc
     2581 gcagcaggat tttttcagcg acagtcatct ggtcatgttc aaaccacggt ggttgtccgt
     2641 cattgccccg ctgaagtgtt aacgggcatc cacaggacac acagcacaaa acgtcctgtc
     2701 ggttgtgctt cgcagcttct ttcgccgtaa caaaatggcc tttgctgtca ttcgccatat
     2761 aacagttcag aattcgcata tttcctcctg agttgtccgg ggaatactga atcctccggt
     2821 ctggcagtgt gtgcagtact gctccccata ataatcgtgg tgacattgtc tgcagtgcca
     2881 gctggcttta cgcaccacgg gtaaggcatc cggtacgaat tgctgcagac gtttaatcaa
     2941 ctgtatttct ctgcgctccg gcctgacata agggcactgt tgaccgtgct ctgtcagcct
     3001 gtcgtcagtg tgttcaaacc agggaagttc agtttcgtat tgcggatggt atctgagtgc
     3061 actgccacac aagtggcagg tgtagttagc ataaggtgcg gcctgtgcag tacgggcgcc
     3121 cgtcagacgt ccgttgccat caagagcgat aaaggatttt gcgtacatag tcatatacct
     3181 cagccgccag acgacacgca ggcaacagcc gtcccggtac gggcagcgtg gtcagggtgt
     3241 gaatggcggt tcagttaaaa tctggactgg agtttgggta aagcaggctg gttgctgtta
     3301 cagcactgtg gttgtcacac aatggaacac tgacgtaaaa aacagtcagt gggcgagtgg
     3361 atacatcgtt gccgccagac aacaacccat acttttggtt tgccggatat tggcgcgcca
     3421 gtcgtgctca actccggcta cagccaaaat tgcgctcacc ggtgcagtga gctttggtat
     3481 gctccttcgc cagatagtca gcacgttcca gcacttgctg aaagccggtg tcatcattac
     3541 gcgccagcca caccgccggc caggccggaa aatgcaccag cgtggcatgg cacccgacat
     3601 ccactcccag ggcactgcac cacgcctgtt taatcatccc ggccagagac tccggagcgc
     3661 ggtaatcacc ggcacgacac caggtatccc ggttgaccag cagcatcagg tgatagtgtt
     3721 ttttaccttt gcactcccca aactcccggg cccagacgta atgcagggtt gtgggatgca
     3781 cgcgtttacc ttcccgatgc ttacgtttct ggtaagcgtc gattcgggct ttcagggcat
     3841 tgatgaagcg ggatatcaca gctgcgtccg ttgctgccgg tacatccggg agacgcaggt
     3901 caacccgcaa tgccgtcagg cggggatgaa cattcagtgc gtgccgcacc gtctcacgaa
     3961 tacgttgctg ccagaagggg ttgtatttgt aggtcatggt taaatctccg tatggttcat
     4021 acggaatagc cacgtcgtaa aaaatacgca gagtccctga agtgacctcc ggcaggacac
     4081 tgtctcaggc actctgtgat aacccgaacg gtcgtctccg gactgacggt tgaattttca
     4141 gcgtcgtttt ttaatgtaaa aaaccggtta cgggtaaggc tgtgaggagg gagtgatggt
     4201 gatacgcaaa aagaagtgca gggactgtgg aaacgcgatt actcataata cagtgtgttg
     4261 cccgtattgt ggttctgtcg accccttcgg ctactaccgg aatacagacc gtatcgttac
     4321 catactgctg gcgctgatta ttgtggtcct gctgactact gtcagcgtca gtgtgtgtat
     4381 actgtgtagc tggtgagata acggttcatg tatcccctga gctgacagtg taccgtgtat
     4441 attaagcgta accgacaggt tctccttcat accatattaa attttccagg gattgccgct
     4501 gccttatttc gtcccataca ggaaaactca tctgggtttc tgacaaatac gcgaaactca
     4561 cagcccctca atatcaacca cacgttgtaa gccggtaata cctgaaaatc agcctattac
     4621 cgcccacgcc tccggcacca ttcccttttg atttcgaatt ctttgttgtc gctataccgc
     4681 ttaatgttcc cggtcctccg gtgcagataa caccggagtc atcgtagcgc tgcttcaata
     4741 ttcaggtgac gtatttccag acggaagccg gcgcgcgatc gtaaagtttg ttcattgtca
     4801 gttctgccag ccggttgtca acggcagagt taaaggcttc cagctccgca agggtaagtt
     4861 tataacgcgt gtgggaattc acttttcccg cgtctcctga cgtattacgg gctgccactt
     4921 acctggtgtc ccgcaataca gccttagtgt tccgttatcg gggatccgga cgcaccgcaa
     4981 caaccgggga agtatttctg gtttagagca gtgtcaagag ggatttctca gcccctcact
     5041 caccggctca gtctcagggc aggagcatta atacgctcag ttcatgtctt atacaggaat
     5101 gggcacaggg ggagctggcg tgtcactgct gattttacgc atcagcgttc atgtatctgc
     5161 actctgatac atgaacgatt cacagcgctc tgttaccgga gagatttact ctcttagctt
     5221 actctggcaa atcctttacg ttactctctg atgacttctt atatttataa taccaatcag
     5281 acagccttat cccatcggtg atatcagtct cctcctcgcc aggaacgggc tgactcactg
     5341 taactacctc cgaaaacaac tctgcaatta tcagaacgcc tgcttctctc cctgtcctca
     5401 cgaaaactat cccctcttta tcgcgcgtgc gtgcggaagc atcttttcgc aacaaccacc
     5461 cggtataccg gtacggctca gccaccgcaa cctccccgtt tatctcctgg cggtcacatt
     5521 cccgattatt ttttacgttt ctccccggtt gttatgccgg tgaaggtggc gcgctgtttt
     5581 catgaccaca ccggctggtt taacaacatc cggaggaaca ttctcatggc taccccagtt
     5641 tcactgatgg atgaccagat ggtcgacatg gcatttatca ctcaactgac cggcctgaca
     5701 gataagtggt tttacaggct catcagggat ggggcctttc cggctcccat caagctgggc
     5761 cgcagctccc gctggctgaa aagtgaagtg gaagcctggc tacaggcgcg tattgcacag
     5821 ccccgcccgt aatttctgac cgttatccgt tcacccgcag cagacgtaat ccccggcctg
     5881 ccggcggcat tctgctgcct gttatatccc cgtgaggaat atgaaaatga aacaacagta
     5941 tcagacccgc tacgaatggc tccacgaaag ctaccagaaa tggctgaccg gcttcacccg
     6001 gcacgccgta tcctggggcg tgtgtcatcc gaatatctac tacttccata atctgacgcc
     6061 cggttgggtg tcattcaatg gtgaaaaacc ggagattgcc atcgtacccc agagcctgca
     6121 ccggctgatt tatggccctg acaaacgggc cacgccgccc ctggatgatg atctgatagt
     6181 gaatttatgc accagtgaac atctgctggt tcatcatccg atgctggaag gcattctcct
     6241 gtctgaatgt gaacgtctga ggcagcgttc tctggcaaat aaactgatca gcctcttccg
     6301 tcagtttggt ggcacggagt tgcgcctcaa actggtctgg ctttgctggc ttgatttaat
     6361 gaccggaaac agccttgaag actggaaaga gaacctgaaa cggaaatcag aaaaagagct
     6421 ggaggaatgg atcatcaacc gtcagaggca gagcgcagca ctgacggatc tgatggatca
     6481 gtacgtgctc ctggcatacc gcacaacggt tgacgatagc cgcaactgat gccggttgct
     6541 tccggaaggt gtgacaaaat acggaaatga tgatgctccc ggcccccatg ctcacctgca
     6601 gagcgtgttc gtcgtcatca tactgagcca tgacgggcgt cttaagggca tcctgtcatt
     6661 gctgagcagg caacaccacc agaggagcca cggacaggga tgttctttac agcccgatac
     6721 acgccaggtt ctcagcagac tgaatgtgca ggtgcggtta ttcaccagaa atggcatgct
     6781 gcggtacata cccatcctgg tgacaatatg tacgtggcga cagcgatccc ggcactgtgg
     6841 aagagactca ctcagcctgc aggatattct ttccggcatc cgggaatgtc cgcctgaatg
     6901 caaacaggat gatatttgaa tccgtaatac tgttctttca tcataaataa tttatgcaga
     6961 tacagttaac cgttacagta gccggtttgt tttaccggga aataatatcc gtcagactga
     7021 taccggaaca tatctgaaac acgttatgat gttttgttat cagaaatgtt gtatattctt
     7081 atccgtggcg acagtttaat gccctctggt atcacctgat aatgtatatc ctgcgatgat
     7141 tcgttatatt tacgggacac gcctcttttc attgtgctcc ttttcagact tcattttccg
     7201 taagtgttcc ccggcataaa ggaggtacct ttctggcgtt tataactctc tttatcggtg
     7261 acgccggtaa tacagggaat accacaccga caaacgaggt attaacagac gatatttcag
     7321 tgtccataat ttttctgtcc ggttaaataa cgtcagtctg ctgccctctg actgtaatca
     7381 tcaaagccgg aatgcatacc gggttacaac gtaaaaaaag tgatgctggc ccccggtgca
     7441 gctggcgggc gaaacatgtt tacctttcaa taccccctgt ctgcagtctc cactcttcca
     7501 tcctcttaag ctacgtcggc cacatgagtg cacttccatg ataacaggta ttcctgtcag
     7561 cataacgaag ccggttacct tcccttcctc ctgcatttcc tgtcagaatg actatacagc
     7621 gcgcagattg cctttttatt gaccatctta atataccgtt ataaacttct ttttatcata
     7681 ttattatcac cttattatgt ttaattttta agcagtcgga gaaagaggat ggttcagaaa
     7741 attttgtcgg ataaggttat gaatgaaaga acaaatgcgt attactctta ttatcttgga
     7801 gagcgtaata tctccgtact gccccttaat gtttatgatc ccccggaacg ttttatcgct
     7861 tacataaaaa aaaacagaga aaatctgaac atcacacttt cagattttga acttgaacaa
     7921 ataatttctg gtatgcgtct gaaggccctg gccttcctgg tccccctgga aaaaatatcc
     7981 tggatcgcgg gcagtgaacg cgcatgctta ttttcctggt atctgttgat gcagtttatc
     8041 cagaataaca gggcaaaaat aagcgcagac ttgcttcaaa agaataaact gtatcttaaa
     8101 gaagagtatc tggaagggaa tgccttcccg tcggactcat cgacacagtt cagacaaata
     8161 ctcagagttc tcgatattct ttctgataag aatttgcggg atgagtggat aatccagacc
     8221 aaagacagat ggatgcgggc atttaaatca aagagtcctt tcagttacct cctcccggag
     8281 aacgaacatg aatgcatatg gacatggaat tatttgaagg gaaaaaatat tgcactggaa
     8341 aaactggcca gttttcctgg ttctgctgat atttaccacg caattcacct ttcatttgac
     8401 atatgggtta cctgtccact cacctcacct gacgatataa aaaatttcag aaacagtttc
     8461 aataaggcca aggcgcagcg caagtacaaa aaaatgcagg aagataaagt caatgtacag
     8521 ttttttcttg acgcagaaac cagggcgcag ctcaaagaat tatcgagggt cagaagactc
     8581 agtaccggcg aaatgctgca tgacctgatt gttgaagagt ataaaaggta ccgacattcc
     8641 agataatttc agacatatat tatctccgtg agtcccccgc cacctttccg gtgtgc
//
LOCUS       DAJTMX010000157         8683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTMX010000157 DAJTMX010000000
VERSION     DAJTMX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.157"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO145_003628"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO145_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5468611.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO145_003629"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO145_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5468612.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO145_003630"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO145_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO145_003631"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO145_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5468613.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO145_003632"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO145_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5468614.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO145_003633"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO145_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5468615.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO145_003634"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO145_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5468616.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO145_003635"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO145_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5468617.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO145_003636"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO145_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5468618.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO145_003637"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO145_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5468619.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO145_003638"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO145_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5468620.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO145_003639"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO145_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5468621.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO145_003640"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO145_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5468622.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO145_003641"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO145_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5468623.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO145_003642"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO145_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5468624.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO145_003643"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO145_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5468625.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO145_003644"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO145_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5468626.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO145_003645"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO145_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5468627.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..>8683
                     /gene="rpsH"
                     /locus_tag="OO145_003646"
     CDS             8512..>8683
                     /gene="rpsH"
                     /locus_tag="OO145_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5468628.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKP"
BASE COUNT         2357 a         2030 c         2270 g         2026 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctg
//
LOCUS       DAJTMX010000158         8667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTMX010000158 DAJTMX010000000
VERSION     DAJTMX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.158"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="OO145_003647"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="OO145_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5468629.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3205)
                     /gene="hemX"
                     /locus_tag="OO145_003648"
     CDS             complement(2012..3205)
                     /gene="hemX"
                     /locus_tag="OO145_003648"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5468630.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQGDTPAA
                     APQGE"
     gene            complement(3227..3967)
                     /gene="hemD"
                     /locus_tag="OO145_003649"
     CDS             complement(3227..3967)
                     /gene="hemD"
                     /locus_tag="OO145_003649"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5468631.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3964..4905)
                     /gene="hemC"
                     /locus_tag="OO145_003650"
     CDS             complement(3964..4905)
                     /gene="hemC"
                     /locus_tag="OO145_003650"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5468632.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5292..7838
                     /gene="cyaA"
                     /locus_tag="OO145_003651"
     CDS             5292..7838
                     /gene="cyaA"
                     /locus_tag="OO145_003651"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5468633.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7878..8198)
                     /gene="cyaY"
                     /locus_tag="OO145_003652"
     CDS             complement(7878..8198)
                     /gene="cyaY"
                     /locus_tag="OO145_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5468634.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8246..>8667)
                     /locus_tag="OO145_003653"
     CDS             complement(8246..>8667)
                     /locus_tag="OO145_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5468635.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1973 a         2238 c         2229 g         2227 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggttt agcttccgtt gtccccgctg
     2101 ccggttgtgc cagcaggtta cgcacgcgag tctgcatcag tttttccagc atcgcctggc
     2161 tttgcagggt ttccggaaga tccatcgaga tattttgctg gcttaactgg tccacctcgt
     2221 cgaggaacgc tttggtggtg gcatcatcag tatcgtagta agcacgtacc caggtggaga
     2281 cgttctccag cgcctggcga taagtctctt cctgatgacg cggtacagct tgtgctgcga
     2341 ccagcaggcg agagcgaata ttttcgcgca gatagatatc ctgatttggc gctaacagcg
     2401 gtacggcggt gtcatcacga cggcgaatcg taatgaagtt gtccataaag ttctgccagc
     2461 ttttttgcag attgatacgc cattcgctga tggaactgga aagctcttca ccgtctgaat
     2521 ccatcggcga accatcgctg tcattatcgg ccagacgcag gttatctacc tgatttgaaa
     2581 gctgattaag cttaaggatg atgccgtcat aatccacctg cgatactgca gaaaggctgg
     2641 cgatatcatc ggtaattgcc cgacgaacgg taatcagact cgggtcattc atatccgcca
     2701 ggctggcgtc tgcacttttc agcaacgctg cagaggtcgt gacgtcctga tcgctccaca
     2761 gcttccgtcc ggcgagtttc accagaaaat cggcctgagc cagcagccag gttttagcat
     2821 cgctgccgga aatggtggcg atcttttgtt ggacttcatc caactgtttt gccagcgttt
     2881 cttgctgacg attcgcctgc ttaagctgcg cagcttgttg cttaataatg ccttccagct
     2941 cggctttttg gctctcctgg gctttttgca atgccgtcag ttggttagcc agggcatcgc
     3001 tggtggcggt ctgattgacg gcctgttgtt taccccagcc atacaaaccg acacccgccg
     3061 ccagagcaat agcgatagcc accgcgctga gaatcaatgc agtattgttc ttactctttt
     3121 tttctgttgc gacaggttgt gacgtggtgt ccacggcctc cctggtctct tcaaccacgg
     3181 cggaggtttt ttcttgttcc gtcattatgg cttcctgtta tgagagttat tgtaatgccc
     3241 gtaaaagcgc atcgttgtca gcgttatcgg cgaccttaat gtcttgccag cccagttccc
     3301 gggcgagttt cgccaaacgc tcactgacga ccaatagtcg acagtgtagt aaccagtgct
     3361 cacgatacca ttgtgggatc agcgaccaga gttgctgcaa catttcaccg ctggtaacaa
     3421 cgaccatcgt cacctcgcgg gattgccagc gcatcgcttc ttctgcacca tcgtaatgga
     3481 ttgcgcatcg ttgataacat tcacaaaaag tgacctcagc accgcgcacc gtcagggtat
     3541 ccccaattag ctcacgaccg ccattgccac gtaatatcag cgcacgtttg cccgcaatat
     3601 tttgtaattc aggtaattgt agcaagactt cgctgatttc ccgatcctgt gggtagagaa
     3661 tcttctgtcc gcttacggta tgtagcgcca gtgcggtggt gcgtccaatg gcgaaataag
     3721 taggtagttg gggccattta cgatcttgct gatgcagctg tgattgggca aaagcaaccg
     3781 cgtgttgcga gagggcaaac aacagatcgc tctcccccag cgctgccagt tgatcagcaa
     3841 gttgcggtaa ttgtcgaccc ggagaaaact caatcagcgg aaaatgccag gccacctgcc
     3901 ccagtgtgcg cagacggctc actaactctt ctccagcggg agacgggcgg gtgaccagga
     3961 tactcatgcc ggggcctctc cgttatagac ttcagcgagg atctcgcgcg cgccgttatt
     4021 cagtagctct tctgccagcg aaatccccat ttgttcggca tcttgcggcg caccgcggcg
     4081 ttcaccgcga ataatctgcg aaccgtccgg cgcgccgacc agcgcacata gccagatttc
     4141 gccatcaata agctcggcgt agctaccaat tggcacctga catccgcctt cgagacgggt
     4201 attcatggcg cgttctgcgg taacgcgcag tgcagtttcg tggtgattca gcgcggcaag
     4261 cagctcgcga gtgcgtgtat catcaaggcg gcattcaata ccgactgcac cttgtcctac
     4321 cgccggaaga gaaatctcgg gtggtaacgc ggcgcgaatg cgggactcca gacctaaacg
     4381 ttttagtccg gctacggcaa gaatgatggc atcgtattcg ccgttatcta gtttgctcag
     4441 gcgagtgccg acgttgccgc gcagggagcg gataatcaga tccgggcggc gttcagccag
     4501 ttggcactgg cgacgtaaac tggacgtccc gacgatactg cctgctggta acgcatccag
     4561 actgtcatag ttattggaca caaaggcatc gcgaggatct tcacgctcgc aaatagtgac
     4621 cagccccaga ccttgcggga attcaaccgg cacatctttc attgaatgta cggcgatatc
     4681 agcgcgattc tccaggagcg cgacttccag ctcttttaca aataagcctt ttccgcctac
     4741 tttcgccagc ggcgtatcaa gaatcacatc gccgcgcgtc accatcggta ccagttcaac
     4801 gaccaggccc ggatggctcg ccatcaactt gtctttgaca tagtgtgcct gccagagtgc
     4861 aagtgggctt tggcgtgtgg caattcttaa aacattgtct aacatgcttg ttaccgtcat
     4921 tatcatccgt ggtccatcct aacatccttg ccagagtgat gtcagtgttg tggtgaaacg
     4981 tagacgcctg cgcaaaccgt aaaatgaggt ctggcagtgg atcctgacag gcgtttcacg
     5041 ccgtcgtaat aaggaattta cagagaataa acggtgctac acttgtatgt agcgcatctt
     5101 tctttacggt caatcagcaa ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt
     5161 gaaactaaaa aaaccaggcg cgaaaagtgg taacggttac ctttgacata cgaaatatcc
     5221 cgaatgccgc gtgttaccgt tgatgttggc ggaatcacag tcatgacggg tagcaaatca
     5281 ggcgatacgt cttgtacctc tatattgaga ctctgaaaca gagactggat gccataaatc
     5341 aattgcgtgt ggatcgcgcg cttgctgcta tggggcctgc attccaacag gtctacagtc
     5401 tactgccgac attgttgcac tatcaccatc cgcgaatgcc gggttacctt gatggtaacg
     5461 ttcccaaagg catttgcctt tacacgcctg atgaaactca acgccactac ctgaacgagc
     5521 ttgaactgta tcgtggaatg tcagtacagg atccaccgaa aggtgagctt ccaattactg
     5581 gtgtatacac catgggcagc acctcgtccg tagggcaaag ttgttcctct gacctggata
     5641 tctgggtctg tcatcaatcc tggctcgata gcgaagagcg ccagttacta caacgtaaat
     5701 gcagcctgct ggaaagctgg gccgcctcgc tgggtgtgga agtcagcttc ttcctgattg
     5761 atgaaaaccg cttccgtcat aatgaaagcg gcagcctggg gggcgaagat tgtggctcca
     5821 cccagcatat actgctgctt gacgaatttt atcgtactgc cgtgcgtctc gccggtaagc
     5881 gtattctgtg gaatatggtg ccgtgcgacg aagaagagca ttacgacgac tatgtgatga
     5941 cgctttacgc gcagggcgtg ctgacgccaa atgaatggct ggatctcggt ggcttaagct
     6001 cgctttctgc tgaagagtac tttggtgcca gcctttggca gctctacaag agtatcgatt
     6061 ccccatacaa agcggtgctg aaaacactgc tgctggaagc ctattcctgg gaatacccga
     6121 acccacgtct gctggcgaaa gatatcaaac agcgtttgca cgacggcgag attgtatcgt
     6181 ttggcctcga tccatactgc atgatgctgg agcgtgttac tgaatacctg acggcgattg
     6241 aagatttcac ccgtctggat ttagtacgtc gctgcttcta tttaaaagtg tgcgaaaaac
     6301 tcagccgtga acgcgcctgt gtaggctggc gtcgcgcagt gttgagccag ttagtgagcg
     6361 agtggggttg ggacgaagct cgtctggcaa tgctcgataa ccgtgctaac tggaagattg
     6421 atcaggtgcg tgaggcgcac aacgagctgc tcgacgcgat gatgcagagc taccgtaatc
     6481 tgatccgctt tgcgcgtcgc aataacctta gcgtctccgc cagtccgcag gatatcggcg
     6541 tactgacgcg taaactgtat gccgcgtttg aagcattgcc aggtaaagtg acgctggtaa
     6601 acccgcagat ttcacccgat ctctcggaac cgaatctgac ctttatttat gtgccgccgg
     6661 gccgggctaa ccgttcaggt tggtatctgt ataaccgcgc gccaaatatt gagtcgatca
     6721 tcagccatca gccgctggaa tataaccgtt acctgaataa actggtggcg tgggcatggt
     6781 ttaacggcct gctgacctcg cgcacccgtt tgtatattaa aggtaacggc attgtcgatt
     6841 tgcctaagtt gcaggagatg gtcgccgacg tgtcgcacca tttcccgctg cgcttacctg
     6901 caccgacacc gaaggcgctc tacagcccgt gtgagattcg ccatctggcg attatcgtta
     6961 acctggaata tgacccgaca gctgcgttcc gcaatcaggt ggtgcatttt gatttccgta
     7021 agctggatgt cttcagcttt ggcgagaatc aaaattgcct ggtaggtagc gttgacctgc
     7081 tgtaccgcaa ctcgtggaac gaagtgcgta cgctgcactt caacggcgag caatcgatga
     7141 tcgaagccct gaaaactatt ctcggcaaaa tgcatcagga cgccgcaccg ccagatagcg
     7201 tggaagtctt ctgttatagc cagcatctgc gcggcttaat ccgtactcgc gtgcagcaac
     7261 tggtttctga gtgtattgaa ctgcgccttt ccagcacccg ccaggaaacc gggcgtttca
     7321 aggcgctgcg cgtttctggt caaacctggg gcttgttctt cgaacgcctg aatgtatcgg
     7381 tacagaagct ggagaacgcc atcgagtttt atggcgctat ttcgcataac aaactgcacg
     7441 gcttgtcagt gcaggttgaa accaatcacg tcaaattacc ggcggtggtg gacggctttg
     7501 ccagcgaagg gatcatccag ttctttttcg aagaaacgca agacgagaat ggctttaata
     7561 tctacattct cgacgaaagc aaccgggttg aggtgtatca ccactgcgaa ggcagcaaag
     7621 aggagctggt acgtgacgtc agtcgcttct actcgtcatc gcacgaccgc ttcacctacg
     7681 gctcaagctt catcaacttc aacctgccgc agttctatca gattgtgaag gttgatggtc
     7741 gcgaacaggt gattccgttc cgaacaaaat cgattggcaa cctgccgccc gccaatcagg
     7801 atcacgatac gccgctatta cagcagtatt tttcgtgatg agcgtgccgg aaagcgaggc
     7861 ttatccggca tgcaatctta gcggaaactg actgtttcac ccgcctgttg cgtcgccgcc
     7921 tgttccagca aatcccagaa ggtttcgccg ctgcgatcgc aaatccattc atcgcctttc
     7981 aggtcaaaat ggtagccgcc ctgtttggtt gccagccaca cctggtgcag cggctcctgg
     8041 cggttaataa tgattttgct gccgttttca aaggtaatgg tcagtacgcc gccgttgatt
     8101 tcgcagtcga tatcgctgtc gccatccctg tcgtccagac gctcttcaat ggtcagccac
     8161 agttgatcag ccaggcgatg aaattcactg tcgttcattg ttgtatcctg tttttaagtg
     8221 atggcggcag tatagcggca tggggtcagg gcttcaaagt ttgcacctct gctgctgcgt
     8281 tccggcatga ttcatccgtc accggaataa tgatgtctct gttttgaact tcacgtacgt
     8341 tctcctctct gacacgagtt ttcgccgggc agggggaggg cgtaatcctt acctctgtat
     8401 cggtccatgt ataaacattg cgatcgtcat aagcgaagcg atcgttttta gttagcgaac
     8461 cgttaactgg aataatttgc aagctctttg accgtaattg gcggataaag taagcatgct
     8521 gtttgccgct attaaatgag cgatcaatga ccaggtctgg cgtatccagc gggattatct
     8581 caagctggtc ttcaccaaat gtcgataatt ttgttacata cagccgatct ttatattgcg
     8641 caatattgcc agtaatcaga tggaatt
//
LOCUS       DAJTMX010000159         8574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTMX010000159 DAJTMX010000000
VERSION     DAJTMX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.159"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..723
                     /locus_tag="OO145_003654"
     CDS             <1..723
                     /locus_tag="OO145_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5468636.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRT
                     ALRAVRDTVVSVARAVWNWIFF"
     gene            1057..4178
                     /gene="ag43"
                     /locus_tag="OO145_003655"
                     /pseudo
     CDS             1057..4178
                     /gene="ag43"
                     /locus_tag="OO145_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
     gene            4299..6815
                     /locus_tag="OO145_003656"
     CDS             4299..6815
                     /locus_tag="OO145_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468637.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            6891..7346
                     /locus_tag="OO145_003657"
     CDS             6891..7346
                     /locus_tag="OO145_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468638.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(7465..7676)
                     /locus_tag="OO145_003658"
                     /pseudo
     CDS             complement(7465..7676)
                     /locus_tag="OO145_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7757..>8574
                     /locus_tag="OO145_003659"
     CDS             7757..>8574
                     /locus_tag="OO145_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5468639.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         2035 a         2262 c         2428 g         1849 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcacaccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgctt ccgtctgagt ggtcacggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg ggccctgtct
      241 gtggatgagt atttctggcg acacatcctg cagtgcggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ctgacaaaac ggaaccctgc catgaatggg atatggcagg tattcagcct
      361 tctcccgcac aggaacagaa cattcgtgag aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctca tgacagcgct tcccgaccat gccgccagcc ccctgatgac ccgactgcag
      541 gacgagctgc gcacggagtc tgtccgctct caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg tacggccctg
      661 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gatcttcttc
      721 tgaacctgtt gtggatgatg tcctccctgc ctctgagtct gctcacaaaa gcgctgtttt
      781 cgttactgtc tctcttgtcc gtgcaataga ctgataatag aataaaacga tcaataccta
      841 ttttaccgat cgttaatatc gatcgttatg ctaataataa ctcctgttag caacgtgcgc
      901 agatacacac agacatgaga ttcagggaac aacagagcca cacgtcagaa acttccgtca
      961 gccggacctc cggcactgta accctttacc tgccggtatc cacatctgtg gataccggct
     1021 tttttattca ccctcactct gattaaggaa atgctgatga aacgacatct gaatacctgc
     1081 tacaggctgg tatggaatca cattacgggc gctttcgtgg ttgcctccga actggcccgc
     1141 gcacagggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcggt cacgtcactc
     1201 ccggtgctgg ctgctgacat cgttgtgcac ccgggtgaaa cagtgaatgg cggaacactg
     1261 gtaaaccatg acaaccagtt tgtatccgga acagctgatg gcgtgactgt cagtaccggg
     1321 cttgagctgg ggccggacag tgacgaaaac accggcgggc aatggataaa agcgggtggc
     1381 acaggcagaa acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc aggaggaact
     1441 gccagtgata cggttattcg tgatggcgga gggcagagcc ttaacggact ggcggtgaac
     1501 accacgctgg ataacagagg tgagcagtgg gtacacgggg gagggaaagc tgccggtaca
     1561 attattaacc aggatggtta ccagaccata aaacatggcg gactggcaac cggaaccatc
     1621 gtcaacaccg gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg tcagatggtc
     1681 ggagggacgg ctgaatccac caccatcaat aaaaatggcc ggcaggttat ctggtcttcg
     1741 gggatggcac gggacaccct catttacgcc ggtggtgacc agacggtaca cggagaggca
     1801 cataacaccc gactggaggg gggtaaccag tatgtacaca acggtggcac ggcaacagag
     1861 acgctgataa accgtgatgg ctggcaggtg attaaggaag gaggaactgc cgcgcatacc
     1921 accatcaacc agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc tgatgtcacc
     1981 cagaacacgg gcgtgcactg gttaccagta cagctgcaac cgtcaccggc acaaaccgcc
     2041 tgggagcatt ctctgttgtg gagggtaaag ctgataatgt cgtactggaa aatggcggac
     2101 gcctggatgt gctgaccgga cacacagcca ctaatacccg cgtggatgat ggcggaacgc
     2161 tggatgtccg caacggtggc accgccacca ccgtatccat gggaaatggc ggtgtactgc
     2221 tggccgattc cggtgccgct gtcagtggta cccggagcga cggaaaggca ttcagtatcg
     2281 gaggcggtca ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg
     2341 gtgatacggc cacggatacc acggtaaatg gcggactgtt caccgccagg ggcggcacac
     2401 tggcgggcac caccacgctg aataacggcg ccatacttac cctttccggg aagacggtga
     2461 acaacgatac cctgaccatc cgtgaaggcg atgcactcct gcagggaggc gctctcaccg
     2521 gtaacggcag cgtggaaaaa tcaggaagtg gcacactcac tgtcagcaac accacactca
     2581 cccagaaagc cgtcaacctg aatgaaggca cgctgacgct gaacgacagt accgtcacca
     2641 cggatgtcat tgctcagcgc ggtacagccc tgaagctgac cggcagcact gtgctgaacg
     2701 gtgccattga ccccacgaat gtcactctcg cctctggtgc cacctggaat attcccgata
     2761 acgccacggt gcagtcggtg gtggatgacc tcagccatgc cggacagatt catttcacct
     2821 ccacccgcac agggaagttc gtaccggcaa ccctgaaagt gaaaaacctg aacggacaga
     2881 atggcaccat cagcctgcgt gtacgcccgg atatggcaca gaacaatgct gacagactgg
     2941 tcattgacgg tggcagggca accggaaaaa ccatcctgaa cctggtgaac gccggcaaca
     3001 gtgcgtcggg gctggcgacc agcggtaagg gtattcaggt ggtggaagcc attaacggtg
     3061 ccaccacgga ggaaggggcc tttatccagg ggaataagct gcaggccggt gcctttaact
     3121 actccctcaa ccgggacagt gatgagagct ggtatctgcg cagtgaaaat gcttatcgtg
     3181 cagaagtccc cctgtatgcc tccatgctga cacaggcaat ggactatgac cggattctgg
     3241 caggctcccg cagccatcag accggtgtaa gcggtgaaaa taacagcgtc cgtctcagca
     3301 ttcagggcgg tcatctcggt cacgataaca acggcggtat tgcccgtgga gccacgccgg
     3361 aaagcagcgg cagctatggc ttcgtccgtc tggagggtga cctgctcaga acagaggttg
     3421 ccggtatgtc tgtgaccgcg ggggtatatg gtgctgctgg ccattcttcc gttgatgtta
     3481 aggatgatga cggctcccgt gccggcacgg tccgggatga tgccggcagc ctgggcggat
     3541 acctgaatct gatacacaac gcctccggcc tgtgggctga cattgtggcc cagggaaccc
     3601 gccacagcat gaaagcctca tcggacaata acgacttccg cgtccggggc tggggctggc
     3661 tgggttcgct ggaaaccggt ctgcccttca gtatcactga caatctgatg ctggagccgc
     3721 aactgcagta cacctggcag ggactctccc tggatgacgg ccaggataac gccagttatg
     3781 tgaagttcgg gcatggcagt gcacaacatg tgcgtgccgg cttccgtctg ggcagccacc
     3841 acgatatgaa ctttggtaaa ggcacctcat cccgtgacac cctgcgcggc agtgccaaac
     3901 acagtgtgcg tgaactgccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca
     3961 gctcccgggg ggacatgagc atgggtacag ccgcagccgg cagtaacatg acgttctcac
     4021 cgtcacagaa tggtacgtca ctggacctgc aggccggact ggaagcccgt gtccgggaaa
     4081 atatcaccct gggcgttcag gccagttatg cccacagcat caacggcagc agcgctgaag
     4141 gttataacag tcaggccaca ctgaatgtaa ccttctgata attctgcatt gcctctctgt
     4201 ggtcccggtc atgatgaccg ggacccggac gggggaaaac gtttcagtgc cacattcact
     4261 ggcattcaca ataacatgat attcatcacg gagtgactat gttacagata gtcggcgcgc
     4321 tgattctgct gatcgcagga tttgccattc ttcgcctttt gttcagagca ttaaccagca
     4381 cagcgtctgc gctggcaggg ttcatattgc tgtgtctgtt cggtccggct ttactggctg
     4441 gctatatcac tgaacgcata acccggttat tccatattcg ctggctggca ggcgtatttc
     4501 tgacgattgc cggaatgatc atcagcttca tgtggggact tgatggtaaa catatcgcac
     4561 tggaggctca tacttttgac tctgtaaaat ttattctgac caccgctctc gccgctggtc
     4621 tgctggctct tcccgtgcag ataagaacca ttcagcagaa cgggctcaca cctgaagata
     4681 tcagcaagga aattaacggg tattactgct gtttttatac tgcttttttc cttatggcgt
     4741 gttctgcata cgcaccattg atcgcattgc agttcgatat ttcaccctca ctgatgtggt
     4801 ggggcgggtt gttgtactgg ctggctgcat tagtgacgct gctatgggcg gccagccaga
     4861 tccaggcgct gaaaagactg accagtgcca tcagccagac actggaagaa caaccggtgc
     4921 tcaacagtaa atcgtggctg agcagtttgc aaaacgatta cagccttcct gaaacgctga
     4981 cggagcgcat ctggctgaca ctcatttcac aacggatttc ccggggagaa ctgagggaat
     5041 ttgaactggc agacggaaac tggttactga acaatgcctg gtatgaaaga aacatggcag
     5101 ggtttaacga acagttgaaa gagaacctgt cattcacacc tgatgaactg aaaacgctct
     5161 tccggaaccg cctgaattta tcaccggaag cgaatgacga ttttctcgat cgttgcctgg
     5221 acggcggtga ctggtacccc ttttcagaag gccgccgttt tgtatcattc caccacgtgg
     5281 atgagcttcg tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc
     5341 ataagccggc tccggaatgg tactgctcct ctctttgtca cgaaacagaa acactgtgtc
     5401 aggacattta tgaacgttct tacaccggtt ttatttccga tgcaacggcg aatggtctga
     5461 ttctcatgaa actgccggaa acctggagta caaatgagaa aatgtttgct tccggagggc
     5521 agggacatgg gtttgccgct gaacggggaa accatattgt cgacagagtc cgtctgaaaa
     5581 acgcacggat cctcggtgat aataatgcca gaaatggagc agacagactg gtcagcggaa
     5641 cagaaatcca gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg gcattcgacg
     5701 gacaaaacgg acagtatcgt tacatgggaa atcatggtcc catgcaactg gaagtccccc
     5761 gtgatcagta tgccggcgct gtggaaacca tgaagaataa gatccgcgaa ggtaaagtac
     5821 ccggtgtaac cgatcccgca gaagcgtccc ggctgattcg tcggggacat ctgacttata
     5881 cccaggcccg taatatcacc cggttcggga ccatcgaatc ggtcacttat gatattgccg
     5941 aggggtcggt tgtcagtctg gcggccggag ggatcagttt tgccctgacg gcatcggtct
     6001 tctggctcag caccggcgat cgcgatgctg ccctgcagac agctgctgtc caggcaggaa
     6061 aaaccttcac ccgcacactg gctgtctacg tcacaaccca gcaacttcac cggctcactg
     6121 ttgttcaggg tatgctgaag catattgatt tttcgacggc cagcccgact gtccggcagg
     6181 cgcttcagaa ggggaccggt gcaggaaata tcagtgccct gaacaaagtg atgaagggta
     6241 cgctggtgac atctctggca ctggtagctg tcacaaccag ccctgacatg atcaaaatgt
     6301 tgcggggacg gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttccggtg
     6361 tggcaggtgg tgctgtcggg tcagtggcgg gcgggatatt gttcagtcca ctgggaccat
     6421 ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg
     6481 tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga
     6541 ttcaggagca ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa
     6601 atctgaacgc gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg
     6661 ccggtataca acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca
     6721 tcaggcaacg ccccgtcatg gaatatgatg catcccatct cggcaagatg gttaaccggc
     6781 tggaagaagc attccccccg gagctcccgg cataacgtgt gttctgctcc ttttaggttt
     6841 tatcacctga tttgccagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc
     6901 tgttcaaaac ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc
     6961 gggcacagga tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa
     7021 atatttgtgc ttaccagttc cggctggata atggcggaaa cggtgaagga tttggccccc
     7081 tcaccatcac tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg
     7141 aaacggaagc ttttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg
     7201 agtgcgtgga aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc
     7261 atcgtgtcag tctgccgtta tcggttttcg atccccagga ctaccatcca ctgctgatta
     7321 ccgtttccgg aaaaaatgtt aactgatccc tgaagtcagt ttcatgcttc agcctctgcc
     7381 acaacgtatg gcagaggcta tttatcagga gaaaccagat atgccgggtt acacacagag
     7441 tatgtcctgg ctgaaggatc tttctcatac gggcaggcgg ttgctgtcat aaccgcttac
     7501 cgcaatgtgt ttatccagga tgacccggga atgcatttcc gtcgggttat ccgcaacgcg
     7561 gaaggtcagc gacgctgacg cagccggaac tctgaagctg atgccggaaa acagcttaat
     7621 gcgtggctcg ccagtggagg gcttctcaga caataaactt cattgatttc atccatcagg
     7681 ccgcgtcttc tccgggagac gcggcttttt tcatttgttg caccagtaaa tcttaaccac
     7741 cgataaggag caaagtatgc aattagtcag tcgttttggt tatgcgaacc agatacgccg
     7801 tgaccgtccg ctgacacatg aagagctgat gcaccatgtg ccggggattt tcggggaaga
     7861 gaaacacacg tcccgaagcc agaactatac gtacatcccc accatcaccg tactggaaag
     7921 tctgcagcgg gaaggcttcc agcccttttt cgcctgccag acccgtgtgc gcgacccggg
     7981 ccgccgggga tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga
     8041 acatgtccct gaaattattc tgctcaactc ccatgacggt acctccagct accagatgct
     8101 gccgggttac ttcagattcg tctgccagaa cgggtgtgtc tgtggtcagt ctctggggga
     8161 agtgcgtgtt ccgcaccggg gaaacgtggt ggagaaagtt attgaagggg cttacgaagt
     8221 ggtgggcgtg tttgaccgga ttgaggagaa acgtgatgcc atgcagtcgc tggtcctgcc
     8281 gccaccggca cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca
     8341 tcagcccgtc accaccgccg acattctgac gccacgacgc cgggaggatt acggtaagga
     8401 cctgtggagt acttatcaga ccatccagga gaatatgctg aaaggcggga tttccggccg
     8461 cagtgcaaaa ggaaaacgta tccacacccg tgccattcac aacatcgaca ccgatattaa
     8521 gctcaaccgc gcattgtggg tgatggcaga aacgctgctg gagagcctgc gctg
//
LOCUS       DAJTMX010000160         8501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTMX010000160 DAJTMX010000000
VERSION     DAJTMX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.160"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            44..397
                     /locus_tag="OO145_003660"
     CDS             44..397
                     /locus_tag="OO145_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5468640.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="OO145_003661"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="OO145_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5468641.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="OO145_003662"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="OO145_003662"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5468642.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="OO145_003663"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="OO145_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5468643.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="OO145_003664"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="OO145_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5468644.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="OO145_003665"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="OO145_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5468645.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="OO145_003666"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="OO145_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5468646.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="OO145_003667"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="OO145_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5468647.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="OO145_003668"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="OO145_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5468648.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>8501
                     /locus_tag="OO145_003669"
     CDS             7649..>8501
                     /locus_tag="OO145_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5468649.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGII"
BASE COUNT         1905 a         2309 c         2287 g         2000 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggcattatc c
//
LOCUS       DAJTMX010000161         8373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTMX010000161 DAJTMX010000000
VERSION     DAJTMX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.161"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO145_003670"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO145_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO145_003671"
     CDS             1127..1234
                     /locus_tag="OO145_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468650.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO145_003672"
     CDS             complement(1287..2045)
                     /locus_tag="OO145_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5468651.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO145_003673"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO145_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5468652.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO145_003674"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO145_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5468653.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO145_003675"
     CDS             3758..4618
                     /locus_tag="OO145_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5468654.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO145_003676"
     CDS             complement(4659..5195)
                     /locus_tag="OO145_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5468655.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO145_003677"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO145_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5468656.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO145_003678"
     CDS             6173..6763
                     /locus_tag="OO145_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5468657.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8286
                     /gene="tcyP"
                     /locus_tag="OO145_003679"
                     /pseudo
     CDS             6896..8286
                     /gene="tcyP"
                     /locus_tag="OO145_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2173 a         2004 c         2007 g         2189 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     8281 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     8341 atgttccatc gcggcgatgc atactgactc ata
//
LOCUS       DAJTMX010000162         8332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTMX010000162 DAJTMX010000000
VERSION     DAJTMX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.162"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..159
                     /locus_tag="OO145_003680"
     CDS             <1..159
                     /locus_tag="OO145_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468658.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO145_003681"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO145_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5468659.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO145_003682"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO145_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5468660.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="OO145_003683"
     CDS             875..1246
                     /locus_tag="OO145_003683"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5468661.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="OO145_003684"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="OO145_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="OO145_003685"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="OO145_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5468662.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="OO145_003686"
     CDS             6949..7509
                     /locus_tag="OO145_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5468663.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="OO145_003687"
     CDS             7640..7852
                     /locus_tag="OO145_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5468664.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="OO145_003688"
     CDS             8096..>8332
                     /locus_tag="OO145_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5468665.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1914 a         2085 c         2670 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga taaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc agtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAJTMX010000163         8300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTMX010000163 DAJTMX010000000
VERSION     DAJTMX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.163"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1028
                     /locus_tag="OO145_003689"
     CDS             <1..1028
                     /locus_tag="OO145_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5468666.1"
                     /translation="LVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWF
                     DEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDA
                     EHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIG
                     GMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHD
                     GNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGSFKVFNT
                     CEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPI
                     RPIARRT"
     gene            1029..3194
                     /locus_tag="OO145_003690"
     CDS             1029..3194
                     /locus_tag="OO145_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5468667.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3208..4119
                     /locus_tag="OO145_003691"
     CDS             3208..4119
                     /locus_tag="OO145_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5468668.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4119..5414
                     /locus_tag="OO145_003692"
     CDS             4119..5414
                     /locus_tag="OO145_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5468669.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5459..5689
                     /locus_tag="OO145_003693"
     CDS             5459..5689
                     /locus_tag="OO145_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468670.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5667..6167
                     /locus_tag="OO145_003694"
     CDS             5667..6167
                     /locus_tag="OO145_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5468671.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6167..7585
                     /locus_tag="OO145_003695"
     CDS             6167..7585
                     /locus_tag="OO145_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5468672.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7585..>8300
                     /locus_tag="OO145_003696"
     CDS             7585..>8300
                     /locus_tag="OO145_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468673.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTT"
BASE COUNT         2221 a         2064 c         2185 g         1830 t
ORIGIN      
        1 accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa gatggcattt
       61 caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt gacacaatcc
      121 acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt cttacccgta
      181 ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg atgtctgacg
      241 ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac atgaccatct
      301 atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca tatcccgagc
      361 atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga atcttccaga
      421 taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc tacgtaattg
      481 gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg tgggataagg
      541 aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca gccgttcagg
      601 cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct catgatggaa
      661 accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac gctggattta
      721 tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag ccaggcatca
      781 ctgaattgcg cgacatgatg ctcgatggta gcttcaaagt attcaatacc tgtgagccat
      841 tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc aagcttaacg
      901 acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca aaaatgatgc
      961 gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc atcgcacgga
     1021 gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt gacgcagact
     1081 ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt agccgagtgt
     1141 cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga caattcgatg
     1201 ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct gtcgacgttc
     1261 tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg gggatgtatc
     1321 gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt cgtgagcaga
     1381 tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac gatccaacaa
     1441 gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac gtcatatggg
     1501 acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg attaacgcct
     1561 tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg gataccctgc
     1621 catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat gatgtcgtct
     1681 acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc taccgcgacc
     1741 cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc atcgacgacc
     1801 tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg cgtgtgtata
     1861 agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc ggagagcata
     1921 ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag tgctacgagg
     1981 gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg tcgttcaacg
     2041 ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag caaatcgaag
     2101 gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag aacaggactg
     2161 atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac cctgaagtgc
     2221 cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa gtggctagtc
     2281 ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc aatcaactga
     2341 acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc gcaatgcgac
     2401 gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct cgtcatgtaa
     2461 cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa gttgtcgatt
     2521 accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag tgctatacag
     2581 acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt caggagttgc
     2641 tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa tacttcacgc
     2701 tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa ctggtgatga
     2761 tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag gcacaacaac
     2821 agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg caaggtcagg
     2881 ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct gccaaggttg
     2941 aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat atggacctcg
     3001 acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag caacagcgca
     3061 gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag actcattcac
     3121 aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc ggagtagccg
     3181 agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca ggcttctgaa
     3241 gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt tgtcgataat
     3301 gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga cgatgagaaa
     3361 ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga acgcaaacgc
     3421 cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc ggagcacctg
     3481 cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc cgaagatgca
     3541 ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca ggcaaacagt
     3601 gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca gggtcgcaaa
     3661 actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa gcactacgag
     3721 gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt catgcaactg
     3781 gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc cgccgctctc
     3841 atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat ggacgggcaa
     3901 tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc tcgagccaag
     3961 cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt tgctgcaaat
     4021 atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt agagacgtac
     4081 cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat gaaggtcaac
     4141 tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg ccaatggcgt
     4201 caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc aacaccgtgt
     4261 ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact ggcaacgcaa
     4321 ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac gatttcttcg
     4381 agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc caggcatccg
     4441 ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact gaaatgggct
     4501 cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct ggctgggatt
     4561 ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac atgggcatca
     4621 gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta gatggtgaca
     4681 tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt cagattgctg
     4741 gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca accgcgactg
     4801 gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt gataccgatg
     4861 gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc accaccggat
     4921 ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag atggcgaaga
     4981 atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt actcacatcg
     5041 aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa gagaaggctt
     5101 acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg aacgtggcaa
     5161 caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct cagccgatcc
     5221 cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt ccaggtattg
     5281 gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt aagtgccgta
     5341 ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt ggtctgccta
     5401 accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg agacaagcat
     5461 gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg gctacgactt
     5521 tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt tttcacatcc
     5581 tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc gtaaaaagcc
     5641 tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat ttagttcttg
     5701 cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc gaaccgcagt
     5761 ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt ggcggtgatg
     5821 cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct ccggatccgg
     5881 gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac cttgcctgcc
     5941 gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact gccagatacg
     6001 ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct aaatgtaagt
     6061 ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag tggaatggtt
     6121 ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc ctattcagca
     6181 acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata tcgactatct
     6241 gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat atcttcgctc
     6301 attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg tcgagtacaa
     6361 catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag gagaaagtga
     6421 agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga catcacaggc
     6481 ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca aaaccgtctc
     6541 aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc gcgacattac
     6601 gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt ttatcactga
     6661 ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg agtctcagcc
     6721 tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg gttcatcgac
     6781 tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt atgttgcaca
     6841 accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc cattcgctga
     6901 ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata tcatcgactc
     6961 cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct cctacactgc
     7021 tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg agttgctgat
     7081 tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca atggcccaca
     7141 atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg acttcgttta
     7201 cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga aactgcaatt
     7261 cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc cactcttcaa
     7321 agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg ttgcgcagta
     7381 cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc gtgagcagat
     7441 gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc gagtagggcg
     7501 catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg tcactctgtc
     7561 tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc ggtaatcatc
     7621 caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca gtcgtatctg
     7681 ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc caacgaggcc
     7741 ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat tctcgctgac
     7801 catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga gcgaatcaga
     7861 caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga aagcgatatt
     7921 tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca tgaaacgcgc
     7981 atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta ctctggtgta
     8041 tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac gctcacaccg
     8101 tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt tgtgttcaac
     8161 gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag cggaaccgcc
     8221 aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt cagcagtcgt
     8281 aatgcggcga caacaaccga
//
LOCUS       DAJTMX010000164         8223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTMX010000164 DAJTMX010000000
VERSION     DAJTMX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.164"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..304
                     /locus_tag="OO145_003697"
     CDS             <1..304
                     /locus_tag="OO145_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5468674.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2186
                     /locus_tag="OO145_003698"
     CDS             276..2186
                     /locus_tag="OO145_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5468675.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2187..2390
                     /locus_tag="OO145_003699"
     CDS             2187..2390
                     /locus_tag="OO145_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5468676.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2387..3980
                     /locus_tag="OO145_003700"
                     /pseudo
     CDS             2387..3980
                     /locus_tag="OO145_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3970..5475
                     /locus_tag="OO145_003701"
     CDS             3970..5475
                     /locus_tag="OO145_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5468677.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPEMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5512..5859
                     /locus_tag="OO145_003702"
     CDS             5512..5859
                     /locus_tag="OO145_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5468678.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5917..6945
                     /locus_tag="OO145_003703"
     CDS             5917..6945
                     /locus_tag="OO145_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5468679.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6997..7383
                     /locus_tag="OO145_003704"
     CDS             6997..7383
                     /locus_tag="OO145_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5468680.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7395..7772
                     /locus_tag="OO145_003705"
     CDS             7395..7772
                     /locus_tag="OO145_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5468681.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7759..>8223
                     /locus_tag="OO145_003706"
     CDS             7759..>8223
                     /locus_tag="OO145_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5468682.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1906 a         1932 c         2540 g         1845 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact
     2161 ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc
     2221 aggcgcgcct tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca
     2281 ggatcgaata tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt
     2341 cggttctggg tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc
     2401 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg
     2461 cgcaggattt ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc
     2521 cctgttgcct gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2581 tatcgcggct aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat
     2641 cagctaccgt ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt
     2701 cggggaggtg gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt
     2761 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa
     2821 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     2881 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt
     2941 tctgcgggcc ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga
     3001 ggatgatttt ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac
     3061 cgggcgtccg gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc
     3121 taatcagttt tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca
     3181 gcttcagtcg gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac
     3241 cgaaaaggca tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat
     3301 taatattctg gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt
     3361 taaaattccg cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag
     3421 acaatggatt ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg
     3481 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3541 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3601 ccacgcagat gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc
     3661 cacgggcgcg ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta
     3721 gctccggaag aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg
     3781 aggccggact gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca
     3841 ttttccgcca gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg
     3901 tgtggatagc ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     3961 caacatcacg tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg
     4021 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca
     4081 gcaagccttt cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa
     4141 acggacgagt acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt
     4201 attgctgtac tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt
     4261 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact
     4321 caggtacggg gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt
     4381 gactgcgctg acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat
     4441 gacacggcct gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc
     4501 cagacatccc gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat
     4561 ctggcacagg ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc
     4621 aatcagtttg aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg
     4681 gcgcgccgga tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt
     4741 atgggaacag aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat
     4801 gaattaatca atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat
     4861 acaggaggca ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga
     4921 gtgatgggga tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg
     4981 gcagggcaac agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg
     5041 caacaatctg gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg
     5101 aaaggacgtg aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa
     5161 gcccgtccga ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag
     5221 attatggccc tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt
     5281 ccggagatga ccgtggagaa tgcccgggct gtactggcgg cagcctcagg cagggcagaa
     5341 ccggtttctg catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca
     5401 gcggtacagg gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc
     5461 atggccatgc catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg
     5521 aaaaccatca ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat
     5581 accgccacag gctgcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg
     5641 gatgaggata ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc
     5701 atactggtac tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc
     5761 tttgcgacgg aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc
     5821 tttgccggca gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg
     5881 tagcctgact gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     5941 ttactcggtt ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc
     6001 cgccgtacgg tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg
     6061 ccggtggcgg cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt
     6121 gaaactcgtg tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag
     6181 gcggtggagc gtcttcccgg tgaagatccg gcccagctga acggcccggc ttaccgtcgc
     6241 ctgcgtatca tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag
     6301 atgcaggcgg tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa
     6361 actgttgagg tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg
     6421 gagtggagcg ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt
     6481 gattttgctt ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg
     6541 aatggcttta agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg
     6601 gaaacggcag tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg
     6661 gcaatcgtgg tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg
     6721 ccggacggca cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc
     6781 attcaggatg cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac
     6841 tggctgaccg tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg
     6901 gtgctgccgg atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc
     6961 taaatgctcc cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg
     7021 aaacgtcttt gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg
     7081 gcagatattg cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc
     7141 gcagagccgg atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca
     7201 gttcctgaac agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca
     7261 ctggcgacac tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg
     7321 tttcgtgttt ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag
     7381 taacaggaga tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca
     7441 atgaagtcat tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg
     7501 ggacacacct gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga
     7561 tccggcttga ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac
     7621 gccgtcagga tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg
     7681 atgacggggg atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg
     7741 ccagaatgag gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc
     7801 gatacccgca tggtgccaca gaccagcgca tgggcggtaa accgtgtggc ggcaaagatt
     7861 gtttctgtcg ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa
     7921 ggtattcccc tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga
     7981 aaaatgtatg cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc
     8041 gcacaggtca gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta
     8101 ctgaaagtgg ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc
     8161 tggcatgtga tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc
     8221 ccg
//
LOCUS       DAJTMX010000165         8221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTMX010000165 DAJTMX010000000
VERSION     DAJTMX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.165"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(228..1376)
                     /locus_tag="OO145_003707"
     CDS             complement(228..1376)
                     /locus_tag="OO145_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5468683.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            1691..2317
                     /locus_tag="OO145_003708"
     CDS             1691..2317
                     /locus_tag="OO145_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5468684.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(2353..3144)
                     /locus_tag="OO145_003709"
     CDS             complement(2353..3144)
                     /locus_tag="OO145_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5468685.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(3146..3763)
                     /gene="dmsB"
                     /locus_tag="OO145_003710"
     CDS             complement(3146..3763)
                     /gene="dmsB"
                     /locus_tag="OO145_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5468686.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(3774..6217)
                     /gene="dmsA"
                     /locus_tag="OO145_003711"
                     /pseudo
     CDS             complement(3774..6217)
                     /gene="dmsA"
                     /locus_tag="OO145_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            complement(6457..7749)
                     /gene="serS"
                     /locus_tag="OO145_003712"
     CDS             complement(6457..7749)
                     /gene="serS"
                     /locus_tag="OO145_003712"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5468687.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEVRRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(7840..>8221)
                     /gene="rarA"
                     /locus_tag="OO145_003713"
                     /gene_synonym="mgsA"
     CDS             complement(7840..>8221)
                     /gene="rarA"
                     /locus_tag="OO145_003713"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5468688.1"
                     /translation="TRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPD
                     YDVPVHLRNAPTKLMKEMGYGQEYRYAHDEANAYAAGEVYFPPEIAQTRYYFPTNRGL
                     EGKIGEKLAWLAEQDQNSPIKRYR"
BASE COUNT         2098 a         2192 c         1974 g         1957 t
ORIGIN      
        1 ctgaacattc ccagccatag acaaatcctt cgccaaaatt aaaggggcat ctgtaaaacg
       61 agggattatg cgcggcaaat aagaagtgcg gaaatagcga aatgcaaaat tataacctgc
      121 tgcacatttt atttttaaac gaataaaatt tggaaaaaag caagtcagat agaagaaaca
      181 aaaaagcaga gtgtgaaaca gtaagttcac actttgcttg aattcattta cacgtgagca
      241 acgggtttcg gcgtatgacc ggcgttgcga agcagcatca gcaaatagat aaacgatacg
      301 ctggcgatca tgataaacaa taaattatcg gagaaattct gcattagcat agcgctaaat
      361 gacgggccaa gcagacttcc cacggtatag ctcaacagta aggcttggtt catcgccacc
      421 agttgatgat gttcaacttt ctcacaagcc caggccatcg ccaccggata tagcgtaaag
      481 ccagcggcac cgaggatgaa taacgctggg gccatcgccg cctggctaag catcgcaata
      541 ctgccgagaa tgacgacaaa gacctgaaca cgcaaaacca gcagtcgacc aaacttatcc
      601 gccagacgtc caatcggcca ttgtccgagg atacccgcac tgaccagtac cgccatccag
      661 aaaccaatac tggcattgct cacccctttg tgattgaggt acagaggcat caggccatat
      721 agagatccca gaacgattcc tgagataatg cagccattca cgccaagccg cgcctgacgg
      781 agttttagca ttgccgtaat tgacgtcgaa tcatggtttt cagcctgctg attcaggaca
      841 cgcgtaaaca acagcggtaa gatccctgcc aatgtcaaac ctgtaaccca cggcaatacg
      901 ctcatcagct cggttgagac tttgctgacc agtaactggc ctaaaaacgt tcccacgtaa
      961 taaaccatca tatacgcagc aagcaaacgc ccacggttac gtgacgtccc actgcacatc
     1021 agcgcgctct caacaaccac ccaaatcatg gcacagccga cgcccgcgac aaaacgccac
     1081 gccaaccagc tccagaaacc aatcatcagg ccaaggccgg cacagccagc agcaaaaatg
     1141 aaggaggaca gataatagct gcggttaaag ccaatgcgtt taatgacata ccctgtcagc
     1201 aatgtaccga caaggttgcc ggtaaaatag gatgagctga caacgcctac ctgccatgtg
     1261 gacatgtgtt cctgggcgag ccaaagcggc acgagtgtat ttaacaccgc gatcgccaga
     1321 gtcaacaaaa gcaggccaga cagcaaaagc atgacaggcc gggtatacgt ggacatgaat
     1381 taaaaccgtg aggaagttca aatttcatgc gcatcatgcc accgccaaaa acattgtcaa
     1441 tgggtgaaaa tacgcgcata tcgcagcggc ggtattatcg atttatatta ctcattcata
     1501 acgctaatgc tgaagagatg attaatctca tctgattgtt tttattgatc taacacaaat
     1561 ttcatacgct tcgttcagtc gaaaaatttc atggtcacag caaactcata tttcccgtct
     1621 atgctttcag agcaatgcat cgcgtctgac cgatttcaga cgtaccactg actctcgaaa
     1681 ggagaagagg atgaccaaac cgtatgttcg tcttgataaa aatgatgctg ccgttttgct
     1741 tgttgatcac caggctggtt tactttccct tgtacgggat atcgaacccg ataagtttaa
     1801 aaataacgtg ctggcgctgg gtgacctggc taagtatttc aacttaccaa ctattcttac
     1861 caccagtttt gaaaccggtc ctaacggccc attagttcta gaactgaaag cacaattccc
     1921 cgatgcacct tacattgctc gcccgggcaa tattaacgcc tgggataacg aagattttgt
     1981 aaaagcagtc aaagcgacag gtaaaaaaca gttaattatt gccggtgtgg taaccgaagt
     2041 ttgcgtggca ttcccggcgc tttcagccat tgaagaaggg tttgatgtct ttgtcgtgac
     2101 tgatgcatca gggaccttta atgaaattac ccggcattcg gcgtgggatc gcatgtcgca
     2161 agctggtgcg caattgatga catggttcgg cgtggcttgc gagctgcacc gtgactggcg
     2221 taatgatatt gaaggactgg cgacgttgtt ctctaaccat attcctgatt atcgtaattt
     2281 gatgaccagt tatgacacgt taacgaagca gaaataaaag agaaccgggg ccgaaacccc
     2341 ggttgttact gattagcttg cgacggccat ccccacggtc atatgcaaac catagaatac
     2401 gccgcgaccg attaattccc ctgccagaag cagaatgaat gagacggata gtagcggaac
     2461 cgcaggctga taacctttta gctgtggtgc aatccacaag cacagggcaa cggccaaaag
     2521 cacgatccgc caggacatca gcgcaccata gtccggcacc agcgctgcgg cctgctgcac
     2581 agaactatga atagttgcca gctctgtgcc ctgcatcact gacatcacgc cactcaccac
     2641 cagtgccagt acagaaatgg ctggcagcag acgcatcgcc cagcccatcg gcgtccagat
     2701 gctgtaccag gtcggaacgg tatcaatgct gttatacaca cgcaccatca tccagacaaa
     2761 gacgacgcca agaaccatcg tcaccaccag ccatagcgta cgcaatgccg gtgacagctt
     2821 tttcagcatt gccagcaacc agccgatgcc gcctacagca aaaaagatcg aaccgctggc
     2881 gatttcgtta ctgagtgctg aagcccctac ccggttgagc gagttaaagg cgcgcatcgg
     2941 tgaaccaaga tggagcatag aggcgataaa gccaatgccc atcagtaccc ataagccaaa
     3001 catgcaggcg ataacacgct gctgggcttc tgctcgcagg tcgcctttga gcagcgccaa
     3061 agccagaacg ataaaaccac ccgccacaca ttgcccgaag accgtgaaga tcatcagcgg
     3121 ccattcatgc catccacttc ccatctcaca cctccttcgg gtttgccaga tagccagtgg
     3181 tatccccggt cgggcggcta ttggcattgg gtttgatcac aatattcggt ttggtaaagt
     3241 gagctcgcgg caacggcgca acggccgcca gatcgccatg ttttttacgc agctcgtcga
     3301 tagggccgaa atccagcgcc cgcagcgggc aagattcaac acagatcggc tttttaccct
     3361 ccgcaacacg gtcataacaa ccatcgcatt tggtcatatg gcctttcgtt tcgttatatt
     3421 gcggtgcgcc atacgggcaa gccatatgac agtaacggca gccaatgcat acatcttcat
     3481 cgaccacaac aaaaccatct tcgcgtttat gcatcgcacc gctcgggcag actttagtac
     3541 aagccggatc ttcgcagtgg ttgcacgaaa tcgacagata gtaggcaaac acgttctggt
     3601 gccagacacc gttatcttcc tgccagtcgc cgccagcata ttcataaatg cggcggaaac
     3661 tgacttctgg cgtcaaatct ttgtagtctt tacaagccag ctcgcaggtt ttgcaaccgg
     3721 tgcaacggct ggaatcaata aaaaatccat actgggttgt catcggttac tccttacacc
     3781 ttttcaacct gaacaaggtt tgtatgtgac ggattcccct tagcgagagg agacggacgt
     3841 tgagtggtca gtacgttgat acaaccgccc ttatcgacac gttttgcatc cgggtcatac
     3901 caggcgcctt cacccagtgc gaccacgccc ggcatcattc gtggcgtcac tttcgcctca
     3961 atatgaacct caccacgatc gttaaagatc ctgactttat cgccgttgtg gataccgcgt
     4021 ttttgggcat caagcgggtt gatccacatt tcctgacggc aagccgcttt cagcacatca
     4081 acgttgccgt aagttgagtg aacgcgagat ttatagtgga aacctgtaag ctgcagcgga
     4141 tactgtttgt tcagcggatc ctgataactt tcaaagcccg gcgtgtagat cggcagtgga
     4201 tcgatcacat cgccttctgg caattcccag gtagcggcaa tgtcagccag cgcctgcgaa
     4261 taaatctcaa ttttgcccga tggcgtagtc agtgggtttg cctgcggatc ttcacggaag
     4321 gctttataag caacgtgatg cccttgtggg tcgcgctttt taaagatccc ctgcttgcgg
     4381 aactcttcaa acgttggcag ttcaggaatc gcttcccgcg actgggcata cagatgccgc
     4441 atccactctt cctgggtacg gccttcagta aactgttgct caacgccaag acgttttgcc
     4501 agttcgctgg tcatttcata gatggtctta cattcaaagc gcggtttaat cacctgatcg
     4561 ttgaaaatca cgtaagacat attcccgcag gatgcatcca gtgcaaagtc catctgttcg
     4621 gaagcggtgc agtcaggcag caggatgtca gcatatttcg ccgatgaggt catgtggcag
     4681 tcgataacca caatcagctc gcacttctta tcatcctgga ggatttcatg ggtacggttg
     4741 atttcagaat gctggttaat caggcagtta ccggcatagt tccagatcat tttgatcggc
     4801 acatccagct tatctttccc gcgcacacca tcacgcagcg ccgtcatttc cgggccacgt
     4861 tcaatggcat cggtccacat aaacatcgaa atgctggtct ggatcgggtt ttccaaggtc
     4921 ggcatacgga caaacggtaa gctgtatgaa ccttcacgcg cgccgctgtt ccctccatta
     4981 ataccaacgt taccggtcag aatcgccagc atcgagatag cacgggttgc gatttcaccg
     5041 ttagcgtgac gctgcgggcc ccatccctgg ctgataaacg ccggtttggt actgccgatt
     5101 tcacgagcca gtttgatgat tttgtctgcc ggaacaccgg tgatttgcga ggcccattcc
     5161 ggtgttttag ccacgccatc tggcccttca cccagaatat aagctttata atgaccattt
     5221 ttcggcgcac tggcgggcag ggttttctcg tcatagccaa cgcaatattt atcgaggaat
     5281 gcctgatcca ccaggttttt agtgatcatg acgtacgcca gaccgttaac cagtgcggca
     5341 tctgttcctg gacgaatagg gatccactca tcttcgcgcc cggcaccggt gtcggtatag
     5401 cgcggatcga tgatgatcat gcgagcatta gatttctggc gtgcctgttc aagatagtaa
     5461 gtcaccccac cgccactcat tcgcgtttcg cctgggttat taccaaacag cactaccaat
     5521 ttactgtttt cgatatccga cgggctgtta ccatctgccc agccgccgta ggtatggttc
     5581 aaaccttccg caatttgcgc ggaagagtag tcaccgtaat ggttaagata accgccgcag
     5641 cagttcatca ggcgcgcgac cagggtattt cccggcggcc aggagcgggt catggtgccg
     5701 cccagcgtac cggtgccata gttcagatag atagactcgt tgccgtactc tttgatcagg
     5761 cgctgcatat tggtcgcgat gatgtcgtag gcttcttccc agctaatgcg ctcgaatttg
     5821 ccttcaccgc gcgccccgac tcgtttcatc ggatatttca ggcggtccgg attgtagaca
     5881 cggtgacgca tggaacgccc acgcaggcag gcgcgaacct agtgtaggcc gtcgtaattg
     5941 tcatcgccgg tattgtcgtt tcgacatatt tgatttcacc gtccacgacg tgcatacgta
     6001 gcgggcagcg actaccacag ttaactgtac aggcgctcca gataaccttt tcgtctgatt
     6061 ttgttggaat ggcgctatcg acagcgtgcg caatccgact aaaaggtaat gttaatgcgc
     6121 tgctggccat tgccaggccg ccgatcgctg tcgtttttac caaaccacgg cgactctcct
     6181 cagcagccaa taccgcatca gggattttcg ttttcataat ggctcactca agcttgatca
     6241 cgattgattc cagtaacaaa ccgtattgtg taattatttc tatagcagct cgctatatag
     6301 gcttgtatac atagaataat ttgaaaatca aagagatagg tagattttca ctcgaaagta
     6361 gtattacgtg taagtgagga gtaattattg ttcggtatca aagggagaca aaaaaagcgc
     6421 cctaaaggcg ctttttagat tcagaaaaat tgggtattag ccaatatatt ccagtccgtt
     6481 catatacgga cgcagaactt ctggtacttc aatacgacca tcagcctgct gatagttttc
     6541 cattactgca accagcgtac gaccaacagc cagaccagaa ccgttcaggg tatgaaccag
     6601 acgggttttc ttgtccgact tgctgcggca acgtgcctgc atacgacgtg cctggaaatc
     6661 ccaaacgttg gagcaggaag agatctcacg gtaggtgttc tgtgccggga tccatacttc
     6721 caggtcgtaa gttttgcaag cgccaaagcc catgtcgcca gtgcaaagga tgattttacg
     6781 gtacggcagg cccagcaact gcaggacttt ttctgcatga ccagtcatct cttccagcgc
     6841 cgccattgag tcttctgggc gcacgatctg caccatttca actttgtcga actggtgcat
     6901 acggatcaga ccacgggtgt cacgaccata tgaaccggct tcagaacgga agcatggggt
     6961 gtgggcggtc atcttaattg gcagatcatc ttcatcgatg atttcaccgc gcaccaggtt
     7021 agtcagcgga acttctgccg ttgggatcag cgcatagtta ctggtgtctg cttcttcttc
     7081 cagcggacga gtatggaaca gatcgccagc aaatttcggc agctgacccg taccgtacag
     7141 cgtgtcctgg ttaaccaggt acggaacata gttctcactg tagccatgct gttcggtatg
     7201 cagatccagc ataaactgcg acagtgcgcg gtgcatgcga gcaatctgcc ctttcattac
     7261 cacaaagcgg gaaccagtca gcttaactgc agctgcaaag tcgaggccag agtgcatttc
     7321 acccagcgtc acgtggtcac gaacttcaaa gtcaaactca cgcggggtac cccagcggct
     7381 gacttcaacg ttgtcatttt cgtctttacc taccggcact tcatctgcag gcaggttagg
     7441 gatggtcagc gcgatatcgc gaatttcagc ctgtaaagca tccagctcgg cttttgctgc
     7501 atccagctct tcgcccagtt tgttcacttc cagacgtaaa ggctcgatat cttccccgcg
     7561 cgctttcgcc tggccaatgg atttcgatcg ggagttacgc tccgcttgca ggttttccgt
     7621 tttgacctgc aatactttac gacgcacttc aagagcgccc agcttatcta catccagctt
     7681 aaagccccgg cgtgccagtt tttcagcgac tgcgtctggc tcattacgca gcagattggg
     7741 atcgagcatg cttatcctgt gcttatcgaa ttaaatggga gatgtggcca cagcctgcga
     7801 ccacagggat acagtaacaa cattaccgca acgataacat taacggtagc gttttatggg
     7861 gctattttga tcctgttcag ccagccaggc gagcttttca ccaatcttgc cttcaaggcc
     7921 cctgtttgtc gggaaataat agcgtgtttg tgctatttcc ggcgggaagt aaacctcacc
     7981 ggcagcataa gcgtttgctt catcatgagc ataacgatat tcctgcccgt agcccatttc
     8041 cttcattaat ttcgtcggcg cattacgcaa atgaaccggc acgtcataat ccgggcgttc
     8101 gcgagcatcg gccagcgcgg ctttaaacgc agtgtagaca gcgttgcttt ttggcgcgca
     8161 ggccaggtaa acaatcgcct gagcaatggc gcgttcacct tccgccgggc caacgcgagt
     8221 a
//
LOCUS       DAJTMX010000166         8103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTMX010000166 DAJTMX010000000
VERSION     DAJTMX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.166"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="OO145_003714"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="OO145_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO145_003715"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO145_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5468689.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO145_003716"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO145_003716"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5468690.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO145_003717"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO145_003717"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5468691.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="OO145_003718"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="OO145_003718"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5468692.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="OO145_003719"
     tRNA            7783..7858
                     /locus_tag="OO145_003719"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="OO145_003720"
     tRNA            8016..8091
                     /locus_tag="OO145_003720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
BASE COUNT         1853 a         2090 c         2195 g         1965 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcaacggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agt
//
LOCUS       DAJTMX010000167         8088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTMX010000167 DAJTMX010000000
VERSION     DAJTMX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.167"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..157)
                     /locus_tag="OO145_003721"
     CDS             complement(<1..157)
                     /locus_tag="OO145_003721"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5468693.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEG"
     gene            complement(154..1116)
                     /gene="menC"
                     /locus_tag="OO145_003722"
     CDS             complement(154..1116)
                     /gene="menC"
                     /locus_tag="OO145_003722"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5468694.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(1116..1973)
                     /gene="menB"
                     /locus_tag="OO145_003723"
     CDS             complement(1116..1973)
                     /gene="menB"
                     /locus_tag="OO145_003723"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5468695.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(1988..2746)
                     /gene="menH"
                     /locus_tag="OO145_003724"
     CDS             complement(1988..2746)
                     /gene="menH"
                     /locus_tag="OO145_003724"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5468696.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(2743..4413)
                     /gene="menD"
                     /locus_tag="OO145_003725"
     CDS             complement(2743..4413)
                     /gene="menD"
                     /locus_tag="OO145_003725"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5468697.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(4502..5797)
                     /gene="menF"
                     /locus_tag="OO145_003726"
     CDS             complement(4502..5797)
                     /gene="menF"
                     /locus_tag="OO145_003726"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5468698.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(5876..6181)
                     /gene="elaB"
                     /locus_tag="OO145_003727"
     CDS             complement(5876..6181)
                     /gene="elaB"
                     /locus_tag="OO145_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5468699.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(6236..6697)
                     /locus_tag="OO145_003728"
     CDS             complement(6236..6697)
                     /locus_tag="OO145_003728"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5468700.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            6762..7679
                     /gene="rbn"
                     /locus_tag="OO145_003729"
     CDS             6762..7679
                     /gene="rbn"
                     /locus_tag="OO145_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5468701.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            7870..8058
                     /locus_tag="OO145_003730"
                     /pseudo
     CDS             7870..8058
                     /locus_tag="OO145_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         1874 a         2311 c         2030 g         1873 t
ORIGIN      
        1 tgccctcaac caccccctgc gccgcaaagc ccgtagctaa ctcatcgacg cgagcacaaa
       61 gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct ccccgcactt
      121 gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcct gtacatcagc
      181 gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct gcatcagatc
      241 cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc gcgccagttg
      301 cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc ccagcgcatg
      361 cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg gtttgataac
      421 taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca ggctttcatc
      481 ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc gggttttgca
      541 cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt tggcaaactg
      601 ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat
      661 tgcctccagc aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca atcccacttt
      721 gaccttcgcc actttctcgc ccggcatatc ggcaagtttg aggatcagat cgtccggatc
      781 gccattacac agcggtgccg cacggtagtt ggctgcttgc ggcaacgtat ctgtcagttc
      841 tgccaatgca cagcttacgc caaaggccac ggaaggcatc tgcggtagct cgcaatcgcc
      901 tgccagccag ttatttaccc aggcaagcag cacgctttgc gcctcttccc agttttcctg
      961 gctgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct cacgcaggca
     1021 aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc ccgcgtccat
     1081 ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt aaatttgctg
     1141 aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc ttccgtcatg
     1201 tagaacagca tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc gtcacagtcg
     1261 gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag catttcgcgg
     1321 caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt gacaaggccc
     1381 atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc tcgcgctttt
     1441 ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact
     1501 ttcgggccag tctgaccgaa gatggcatta tctgctgcga tagtcaggtc gcacatcatg
     1561 tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac cggtttcgga
     1621 caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc ggaatcatct
     1681 ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa cgctttatcg
     1741 cctgcgccag tcagaatgat cacgccgata ttgtcgtcat aacgcgcatc tgccagtgcc
     1801 tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg acgattaatg
     1861 gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcgaa accttcggag
     1921 cagtcgtgcc attcaaccgg tgcgtaaggc attgcttcat caggataaat catattgtgt
     1981 cctttagtca gaaacgcaag atctgcgcca gacttgcgat tacgccagcg ggattttccc
     2041 gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt tccgccgcca
     2101 gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat gtgcgggcgc
     2161 taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc atggcggcaa
     2221 gggttgcgcc attattgttg ctgcgcagcg ccaccagctc ccggcgttga tcgtcattga
     2281 gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac ggttctgtgc
     2341 gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcacgttgt tcagcatttt
     2401 gcagccccgg atgcccgcct tcgacaataa ccccacaaag ccccgccagc ccctggcaag
     2461 ccgccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag tcaaggatgt
     2521 tgtaactaac caaggtttta cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa
     2581 tcgccgccga accaccatga cctaggagat caacatacaa ccgtgagtag tcggcaaacg
     2641 cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa aacaccagcc
     2701 agggtaaacc tagttttccg tgttttgcct gcgcgtgcag gatcataaat ggcttacctg
     2761 cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca tttcaatcac
     2821 cgtggtggtt ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct gccagttttg
     2881 cggatgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg
     2941 catcagatag aaacgctcac gctcgctttg cggcgttggc aacagcgaga aaatttgccc
     3001 gccgttgttg ttcaccacaa ttaataccag cggcgcagaa acctgacgca ataacgccag
     3061 cgcgttgaga tcgtaaagcg cggagagatc gcccacaatc gccagcgtcg gtttgccgct
     3121 tgcccgctga acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg cccctcggtt
     3181 gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac gtaccaccag
     3241 gctgttacca acaaacaatt gcccctgttc aggcagatag tcgctgatgc gatgcgccag
     3301 ttgcgcttcg ccaaacgcat cacggcgggc aataaccgcc tgcattgcct gttccgccag
     3361 gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct ccagccagtc
     3421 ggcaatattg gcaattaagc gacgtccgcg atggtgtgcc ggatcaagtc gcccttcaat
     3481 gtcatcaaca atccagtact cttctggttc acagcttgcc tgccattgca ggagccgttt
     3541 ccccgtcagg ctacttccca gttgcaccac aatttgcgcc tgctgcagct cgctggtcgc
     3601 tttggcattg cctaaccaaa gatcggcaca cggcagcggc tgcccggttt gtgacagcac
     3661 atcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt tgccctcttc
     3721 cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga accagtcgcg
     3781 ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt cctgccacca
     3841 gtcacccaga cgctgttgcc agctaagccc ggtatcgtcc atttcgccat acagcggttc
     3901 agcaaacggg cagttgatat ggactccccc cgcatgaagc gtaccgagag cgtggtcgat
     3961 ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg aaatactgtg
     4021 cgtggggtga gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc cgcagtcaat
     4081 tagctccggc gggcgatcgg cggttaagag aatcagtttt tctccggtta acccggcttc
     4141 aatcagtgcc ggatagagat ttgccaccgc cgtgccggag gtcacaatca ccgccaccgg
     4201 ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct catcgaaatg
     4261 ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg tagaacgcga
     4321 gcctggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc
     4381 cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca atattgcggc
     4441 taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat gcgactcatt
     4501 actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt tcctgccact
     4561 cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc acgacattgc
     4621 cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgttgtaat gagagatagc
     4681 ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata aactgtcgcg
     4741 ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt aaacagatca
     4801 catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc actttacgca
     4861 gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt aatcgttggc
     4921 agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc atcagccact
     4981 ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc tactgttccc gccagcgctt
     5041 cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag
     5101 cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt cgacgactgg
     5161 cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt gcccgagcga
     5221 gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg attaattgcg
     5281 tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt aacccaggca
     5341 agggcttgat actcaccagt gtggcgataa attcttttgc ctgaatcgca tcgtgctgaa
     5401 gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag cgtcgccatt
     5461 ccaggcgggg taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc ccccaaatgc
     5521 gtaagtcagc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa
     5581 aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt tgccagtaga
     5641 attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg tctttgagag
     5701 ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc
     5761 gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc tgttaaaagc
     5821 ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac
     5881 ggcgtgccag caacagtcct agtaccagcc cgacggccgc gcccacacca attccttgcc
     5941 agggtttttc gtggacgtag tcatcagcac gataaacagc ctgcttcgcc cgatagtaat
     6001 aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt tctgcatgcg
     6061 ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc acctcttcca
     6121 gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac tgattagaca
     6181 ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagagtt gttgattacg
     6241 cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca tagacctccg
     6301 tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc gccccgaggt
     6361 aaacaggctt atcaggccag tgatgcgtac atgtttcgag tgttttactc atcagctgct
     6421 gccccacttt ttcaccacgc aacgcttcac tgacaatcac ccgacctata acgaccggct
     6481 caagatcatc atcacttttc agaatcctcg catacgccac cagttcatca tttttccaac
     6541 cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga taagggcaat
     6601 tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg
     6661 ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt tagatgtatg
     6721 ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta atttttttag
     6781 gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg cttaacctgc
     6841 aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc
     6901 tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt caccttcatg
     6961 gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc
     7021 aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc gcgctgcgga
     7081 ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct ggcgaaattc
     7141 tcgatgatgg cctgcgcaaa gtaaccgctt atccgctgga acacccactg gaatgttatg
     7201 gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg
     7261 ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca atcacgctgg
     7321 aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca ggtaaagcgc
     7381 tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct aaaggtgtcg
     7441 atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc aatagtcgcg
     7501 gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc ggcaagctaa
     7561 tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg ttacgtgaat
     7621 gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt aacgtttaac
     7681 tctgcttatt attaaaaaca gggtgaaact tgccctgtta tagcaacccg cgctacatat
     7741 atccacccac acaggcatat tgagcgattt ttgatattta ttcatctcct tacgcactat
     7801 attcagagca ataagttgcc tcttttcatc ttgattcagc acaacctttg caactaaaag
     7861 gagcccatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac ccaactcagt
     7921 cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt aaaaaaaaca
     7981 gcgttatccg gagatgaaga aagtattgag ttactgcata atattgcgtt aggttatgat
     8041 gaatttggaa aaaaagctgg gtaatgctgc caacttactg atttagtg
//
LOCUS       DAJTMX010000168         7879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTMX010000168 DAJTMX010000000
VERSION     DAJTMX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.168"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..106)
                     /locus_tag="OO145_003731"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="OO145_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            104..428
                     /locus_tag="OO145_003732"
                     /pseudo
     CDS             104..428
                     /locus_tag="OO145_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(458..3370)
                     /locus_tag="OO145_003733"
     CDS             complement(458..3370)
                     /locus_tag="OO145_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468702.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3373..3489
                     /locus_tag="OO145_003734"
     CDS             3373..3489
                     /locus_tag="OO145_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5468703.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3853..4695
                     /locus_tag="OO145_003735"
     CDS             3853..4695
                     /locus_tag="OO145_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5468704.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4698..5786
                     /gene="capU"
                     /locus_tag="OO145_003736"
     CDS             4698..5786
                     /gene="capU"
                     /locus_tag="OO145_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5468705.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5791..6741
                     /gene="virK"
                     /locus_tag="OO145_003737"
     CDS             5791..6741
                     /gene="virK"
                     /locus_tag="OO145_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5468706.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6806..7750
                     /gene="lpxM"
                     /locus_tag="OO145_003738"
                     /gene_synonym="msbB"
     CDS             6806..7750
                     /gene="lpxM"
                     /locus_tag="OO145_003738"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5468707.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2124 a         1763 c         1866 g         2126 t
ORIGIN      
        1 cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc catgaaagcc
       61 ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc agcggcggac
      121 agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt acccggtcaa
      181 aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt taccacttta
      241 acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag gcctcccgga
      301 agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc
      361 aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg tacttacgta
      421 cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac
      481 ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc
      541 ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc
      601 ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt
      661 taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat
      721 aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt
      781 tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg
      841 cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca
      901 tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca
      961 cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg
     1021 cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat
     1081 taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac
     1141 ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat
     1201 aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact
     1261 tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc
     1321 ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca
     1381 gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct
     1441 ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt
     1501 ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac
     1561 ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac
     1621 tccacagagg atgctccacc tgctgttcaa caagatacac attgtgttta gcacgggtga
     1681 gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt
     1741 cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa
     1801 tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt
     1861 ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct
     1921 gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg
     1981 cattcagttc ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct
     2041 gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt
     2101 tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct
     2161 gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg
     2221 aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact
     2281 gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa
     2341 cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga
     2401 gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga
     2461 catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat
     2521 acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact
     2581 cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt
     2641 cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt
     2701 ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga
     2761 gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag
     2821 cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca
     2881 ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat
     2941 ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat
     3001 caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc
     3061 gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag
     3121 tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc
     3181 ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag
     3241 cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga
     3301 ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct
     3361 tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct
     3421 ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg
     3481 gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa
     3541 agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agtacctgtg
     3601 ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa
     3661 ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga
     3721 tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta
     3781 tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct tgctaggtgc
     3841 cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta
     3901 ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc
     3961 cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg
     4021 gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat
     4081 ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca
     4141 catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag
     4201 gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg
     4261 cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat
     4321 acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat
     4381 cttcggcagg acttgttgag cgggagggaa tatttgaaag aaatgacagg aaaatgcagt
     4441 aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa
     4501 cttggattct attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact
     4561 acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga
     4621 cttttttatt atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga
     4681 ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc
     4741 aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc
     4801 ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca
     4861 cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag
     4921 ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg
     4981 gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt
     5041 tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg
     5101 gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc
     5161 ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg
     5221 ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc
     5281 gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac
     5341 gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt
     5401 atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg
     5461 ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg
     5521 gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg
     5581 gaatccctga tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc
     5641 acgcatggat gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg
     5701 ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa
     5761 tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc
     5821 ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga
     5881 aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat
     5941 tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg
     6001 ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa
     6061 tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg
     6121 tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat
     6181 atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc
     6241 aataaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc
     6301 gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatat tattcggtgc
     6361 gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg
     6421 ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc
     6481 cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc
     6541 tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt
     6601 cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct
     6661 ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaactca
     6721 gaacatcagg acttaaatta aggtttgatg tattctctga ataaaatatt aatgatgatt
     6781 ttggtagggg cattcgcact aaataatgaa aaaatacaaa tccgagttta ttcctgaatt
     6841 taagaaaaat tatctttccc ctgtttactg gtttacatgg ttcgttttgg gaatgattgc
     6901 aggtatttca atgtttcccc cttcattcag agatcctgtc ttggccaaaa tagggcgttg
     6961 ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg attaatttat cgctttgttt
     7021 cccggaaaag agtgatacag aacgggaaat aattgtcgac aatatgtttg ccacagcatt
     7081 gcaatccata gtgatgatgg cagaactggc aattcgtggt ccggaaaagt tccagaaacg
     7141 agtgttctgg aaaggcctcg aaattcttga ggaaattcgg cacaataaca gaaatgtgat
     7201 ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg cagctcaggg
     7261 ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt atgtctggaa
     7321 ttctgtacgg cgtaaattcg gggggcgctt acattcccgg gaggatggga taaaaccatt
     7381 tattcagtca gtacgccagg gatactgggg gtattacctt ccagatcagg atcatggtcc
     7441 tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac caattattgg
     7501 acgtctgatg aacatcagtc aggctatgat tataccgctt ttcccggttt atgatgaaaa
     7561 aaaacatttc ctgactattg aagttcggcc accaatggat gcatgcattg ccagcgcgga
     7621 caataaaatg attgcccgac aaatgaacaa aacagtggaa attttggtgg ggtcacatcc
     7681 ggaacagtat atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc
     7741 gtacccttga aattataaag tacaggtatt tccactagtt gtttcttaca ggttaccaat
     7801 cgaaacacat cccccttccg tgtgttgccc gtttggcacg gaggactgtg tagatgcagt
     7861 tcgttaaatt taatttatg
//
LOCUS       DAJTMX010000169         7744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTMX010000169 DAJTMX010000000
VERSION     DAJTMX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.169"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO145_003739"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO145_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5468708.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO145_003740"
     CDS             1102..2562
                     /locus_tag="OO145_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5468709.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO145_003741"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO145_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5468710.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO145_003742"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO145_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5468711.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO145_003743"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO145_003743"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5468712.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO145_003744"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO145_003744"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5468713.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO145_003745"
     CDS             complement(6727..7245)
                     /locus_tag="OO145_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5468714.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7744
                     /gene="ydeI"
                     /locus_tag="OO145_003746"
     CDS             7521..>7744
                     /gene="ydeI"
                     /locus_tag="OO145_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5468715.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1940 a         1811 c         1957 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgtgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aagg
//
LOCUS       DAJTMX010000170         7611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTMX010000170 DAJTMX010000000
VERSION     DAJTMX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.170"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..452
                     /locus_tag="OO145_003747"
                     /pseudo
     CDS             1..452
                     /locus_tag="OO145_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            512..619
                     /locus_tag="OO145_003748"
     CDS             512..619
                     /locus_tag="OO145_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5468716.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(600..1331)
                     /gene="yehW"
                     /locus_tag="OO145_003749"
     CDS             complement(600..1331)
                     /gene="yehW"
                     /locus_tag="OO145_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5468717.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1336..2262)
                     /gene="yehX"
                     /locus_tag="OO145_003750"
     CDS             complement(1336..2262)
                     /gene="yehX"
                     /locus_tag="OO145_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5468718.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2255..3412)
                     /gene="yehY"
                     /locus_tag="OO145_003751"
     CDS             complement(2255..3412)
                     /gene="yehY"
                     /locus_tag="OO145_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5468719.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3419..4336)
                     /gene="osmF"
                     /locus_tag="OO145_003752"
     CDS             complement(3419..4336)
                     /gene="osmF"
                     /locus_tag="OO145_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5468720.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4588..6885)
                     /gene="bglX"
                     /locus_tag="OO145_003753"
     CDS             complement(4588..6885)
                     /gene="bglX"
                     /locus_tag="OO145_003753"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5468721.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7081..>7611
                     /locus_tag="OO145_003754"
     CDS             7081..>7611
                     /locus_tag="OO145_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR5468722.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFA"
BASE COUNT         1792 a         2239 c         1897 g         1683 t
ORIGIN      
        1 caatctacat agcctgcgaa cgtggatcaa agagcgcggt caggattacc ccgcccagac
       61 actcaccaca catctgttta ttcctctgcg ccgacggctt cagtgccaac aaccgactct
      121 ccaggcgctg ctggcgatcc tcgacggcgt actgatcaac tacatcgcca tttgtctggc
      181 ttcggcacgt aaaaaacagg gtaaagatgc gctggtggtt ggctggaata ttcaggatac
      241 cacccgtctg tggctggagg gctggattgc cagtcaacaa ggatggcgca ttgatgtcct
      301 cgctcgccca ctcgctcaat caactacgcc ctgaactatt cgaaggccgt acattgctgg
      361 tgtggtgcgg tgaaaatcga acctccgccc aacagcagca actcaccagt tggcaagaac
      421 aaggccatga tattttccca ctcggtattt aatgattcgt taacaaatgc gctttactgt
      481 acaatccttt cgttaacata aggagtgcat tatgcgcata gctaaaattg gggtcatcgc
      541 cctgttcctg tttatggcgt taggcggaat tggtggcgtc atgctcgcag gttatacctt
      601 tattttgcgt gctggctaag cgcctgcacc agcctttcaa acaggcggtc tgcgatgatc
      661 gccgccagtg ccaccagtaa cgccccctgg atcacatacg cggtattaaa tccgctaagc
      721 ccgatgatga tgggcgtacc cagcgtactg gcccctaccg ttgaggcgat cgtcgccgta
      781 ccaatgttga taatcaccga agttcgcacg cccgccagaa tcaccggagc cgccagcggt
      841 agctcgacct tacgcagtcg ctgacctcga ctcattccca tacctttcgc aacttctgtc
      901 acgctgggat caatcgctcc cagcccggca agtgtcacct gcaggatggg cagcacaccg
      961 taaaggatca aggcgataat cgctggttgc agaccaaagc cgatcaccgg aacggcaatc
     1021 gccagcactg cgacaggcgg aaaagtctgt ccaacggcgg caatagtttc caccagtggg
     1081 cgaaattccg cgccccacgg gcgagtaaca gcaattccgg cacctgtgcc aatgatcacc
     1141 gcaaacaaac tcgaaattcc caccagccag aaatgagcca gtgccagagc tgcaaaactt
     1201 tcttgctgat aaacgggtcg tggcagttgt gggaacaagg cagcaaacag cggctggctg
     1261 taaggcagcc agaaaatcag cgccacaaaa agagcaatga gccagaacag cggatcgcgc
     1321 aacatcttca tacgcttacg cctccaccag cagatcctga aaatgcagcg tgccgcaagg
     1381 ctggccctgc gtgttcacca ccggcagcac ctcgcatccc cgcgcgacaa acagggagag
     1441 cgcatcgcgt agcgtcatct cttctgccag tgcctcacct tctgcccgtt cttcgcgacg
     1501 cacgtaatcc gccacgctac gtaacgaaag caggtgcacc cccagttcac tacgtccaaa
     1561 aaactggcgg acaaaatcat tcgccggacg agtcagcatc gtcagcggat tcccctgctg
     1621 caccacttca ccgtgatcca tcaataccag atgttctgcc agccgtagcg cctcatcaat
     1681 atcatgagtg accagcacaa tagtgcgccc cagcaaacgg tgaatgcgcg tcatctcttg
     1741 ttgcaacgcg ccgcgcgtta ccgggtccag tgcgccaaaa ggttcatcca tcagtaagac
     1801 ttgcggatcg gcagccagtg cacgcgccac tcccacacgt tgctgctgac caccggaaag
     1861 ctgatgcgga taacgctcac gcaaatttga ctccagcccc agtagcgcca ttaattcgtc
     1921 gatacgatcg tcaatccgcg cccgcgacca tttttgtaat tgcggcacgg tagcgatgtt
     1981 ttgcgccacg ctccagtggg ggaacaggcc aatagattga atggcatagc ccatccggcg
     2041 gcgcaactcc agtactggca gcgagcgaat ttcttctccg gcaaagcgga tcactccgct
     2101 gtcatgctcc accaggcggt taatcatttt cagggtggtg gatttgccgg agccagatgt
     2161 gccaatcagc accgaaaaac tcccttcctg aaaattgaga ttgagatcgt taacggcttt
     2221 ttgtgcgccg aacagtttgc tgacatggct aaattcaatc attacgtttc accttcagca
     2281 gtgcgataag caaatcgaac agcgcgtcgg taagcaccgc cagaacaatt accgggatca
     2341 cccccagcaa cactaaatca atggcgctgc ttagcagccc ctggaaaacc agcgcaccaa
     2401 aaccgcctgc gccgattaac gccgcaatca ccgccatacc tacagtttgc accatcacca
     2461 cccgcaggct gcgcagaaat accggtaacg ccagcggtaa ctgaacatgc aggaaacgct
     2521 gcgccccgct catccccatc gctctggcgc tctccagcac atcgcgcggg atctggttca
     2581 aaccgactac cacgcctcgc accagcggca gcaaggcata gagcaccagc gcaatcagtg
     2641 cgggtgtcat tccggttcct gctatgccga gcttccccag ccacggaaag gccgtaacca
     2701 gcgcggcaag cggcgcaatc aacaggccaa agagcgccac cgaaggcacg gtctgaatga
     2761 cattgagcag agaaaaaatt gccccctgcc gcgcagtgga aaagtagcac cagatgccca
     2821 acggcacacc aatcactaac gcaggcagca ccgcaccaaa cagcaacgtc agatgttgtg
     2881 ccagcgcgtc gtcaaacaca tcctgacggt tggcgtattc tttcattagt gagagatcgt
     2941 taagcgtgcc ggagtacagc aaccacagcg gaataatggc aatctgcata tgcaacaacc
     3001 agcgccacag cggatgcgtg gagattcggc ggatggcatc gctacaggcc agcaatgcca
     3061 gcgccgcagc cagccagaaa cctctgccga ggctggtacg cgccagcgca ctgccatttt
     3121 gcgccagttg ggtcgccgcc tttccagctc cccacaccaa caatacgaag acgaattgcg
     3181 ccagaatgag tgcaaaaatg ctgccttttt tagcggggac aaaacaggct gtcagccagg
     3241 cgcaaccaac gcccaccagc atccagatcg tttgcggcca cagttgccag agatgaagcc
     3301 cttcacccga gactaaacga ttaggcgcgt aactgataaa cggcagcgcc gctgcgattg
     3361 ccgtcagcaa caacagcagc gccagaacag gattaatacg tagataagtc acgggaaatt
     3421 acttcgccca ccctttttgt ttcaggtagt cggcagccac ttttttggca tccagtcctt
     3481 ccactgcaat gctggcattc agttgctgca atgtttttgc atcgaggctg gcgaagactg
     3541 gctgtagcca ctgtgccatt tgcggatact ccttcaacac cgattcacgc accactggtg
     3601 caggcgcgta gataggttgc acgccttgcg gatcgcttaa ggtttgcagc cccagcgccg
     3661 cgaccggacc gtcagtgccg taagccattg cggcattaac gccagaggtt tgctgggcag
     3721 cggctttaat cgtcaccgcc gtgtcgccac cagccagtga cagcaactga tcctgaccga
     3781 gcttaaagcc ataggctttt tcaaacgtgg gtaacgcatc ggcgcgttcg ataaactctg
     3841 ccgaggctgc cagtttgaag gtgccaccct cttgcagata acgactcagg tcggcaagcg
     3901 aagtgagttt gtttttctct gccacatcct gacgcacggc gatagtccag gtgttatttg
     3961 caggcgcggg cgtcagccag attaacttgt tgtgctccgc atcgagcttt ttgactttct
     4021 cgtagccctg cccggcattt ttccacgctg catcgttttc atctttaaag aagaaagcgc
     4081 cattgccggt atattccgga tagatatcca gttcatcgga agtaatcgcc ccgcgcccca
     4141 caggagtcgt tccgagttgc actttattca ccgtcggtac gccgtggctt tcgagtacct
     4201 gcaagatgat gttgccgagc agcgcgcctt cggtatcgat ttttgaaccg actttaacgg
     4261 gggaagccgc ctgtagcggc aggctcacgg ctgccaacat aaccagtgaa cctgcccaga
     4321 gctttaagag tggcatgatg ctttcctcat tcttttactg ttgttttcag cgaattaaga
     4381 gaaaagcata gttgataatg acagagttag cctgagcgga aggattcagt tgcagaatca
     4441 gataaatact taagaggcat attcggtccg gcgtttcctg ccggatgcgg cgcgagcgcc
     4501 ttatccggcc tacaaagggc gaaaattcag caagttaaga tatgaacatc aggcaaaggg
     4561 cgcaaacgtc gcgcccttac taaagcatta cagcaactca aactcgcctt tcttaacgcg
     4621 tgcggaatca gtgccgataa atacattgaa cttgccaggc tcggcgtcat atttcatctg
     4681 ttgattccag aacttcagcg cctcaatatc gatcgggaag ctgacagtct gagtttcgcc
     4741 cggtttcagg gtgattttct caaagccttt cagctgtttc acagggcgac tcatggaagc
     4801 cgtcacatcc tgcaagtaca tctgcactac cgtggccccc tcgcgcttac cggtgttcgt
     4861 cacctgcacg ctggccgtca ctttgccgtc acgcttcatg gtcggcgcag aaagtttcac
     4921 atcagagacg gtgaaagtgg tatagctcag accatagccg aacggataaa gcgctccgtt
     4981 agcttcatca aaataacgcg aagtatattt gttcggcttg tcggcattat acggacgacc
     5041 ggtgttcaga tggctgtagt acaccgggat ctgcccgaca gaacgcggga aggaaattgg
     5101 cagcttgccg gacgggttgt aatcgccaaa caatacatcg gcaattgcat taccgccttc
     5161 agtccccgca aaccaggttt ccagaatcgc atcagcctgc tgatcttctt tcaccagcgc
     5221 cagcggacgc ccgttcatca gcaccagcac cagcggttta ccggtggctt tcagcgccgc
     5281 aatcaagtca cgttggcctt gcggaatagt gatatcggtc cggctggagg cttcgtgcgc
     5341 catcccctgt gcttcaccga ctacagccac caccacatca gattgtttcg ccgtctgcac
     5401 cgcttcatca atcatctctt gcggcgaacg cggatcgact ttgaccgctt cttcatactg
     5461 attcaggaaa tcgataatgc ctttgtcact ggtaacgttc gcccctttgg catacagcac
     5521 tttaccgttt tcaccgacgg aatttttaat cccggtcagt acggtcacgg attgatcggc
     5581 aacaccggct gcggaccagc tgcccatcac gtcacgttta ctgtccgcca gtggcccaac
     5641 caccgcaatg gtggccgatt ttttcagcgg taacgtttcg agacggtttt tcagcaacac
     5701 caggctttcg cgcgccactt cacgcgcttc tttacggtgc aggcggcttt cggcattggt
     5761 atccaccggg tcagactctt tcggccccaa atggctgtat gggtcgttaa acaaccccat
     5821 atcatattta acgttcagta catggcgggc agcatcgtcc agctctgcca tggtcacttt
     5881 gccggattta atcaacccag gcagatactt cgagtagtac tcgtcgctca tactcatgtt
     5941 gattccggat ttcagcgcca cgcgcaccgc atcttccggg tctgccgccg tgccatgttt
     6001 aatcagctct ttgattgcac cgtgatcgga aacggtgatg cctttaaagc cccactggtc
     6061 gcgcagaaca tctttcagca gccaggaatc ggaggtggct ggcgtgccgt tcagcgagtt
     6121 cagcgccacc atcaccgcgc cgctgcctgc gtccagcccc gctttgtacg gcggcatata
     6181 atcattaaac aggcgctgcg gactcatatc gacggtgttg tactctttac cgccttctac
     6241 cgcgccgtat gcggcaaagt gtttgacgct ggtcatcacc gagtaacgat ctgccgggct
     6301 tttaccctgc atcgcttcca ccatggtttt acccattatt gaggtgagat acgtatcttc
     6361 gccaaaacct tcggaagcac gtccccagcg cggatcgcgc gagacatcga ccatcggtgc
     6421 ccaggtcata ttcaggccat catctgccgc ttcataagca gagacacgcc cgaccgtttt
     6481 caccgcatcg aggttaaaag acgaggccag accgaggcta atcgggaaca ccgtgcgctg
     6541 accgtgcagc acgtcgtaag caaagaaaag aggaattttc aggcggctta attccatcac
     6601 ctgatcctgc atggcgcgga tatcctgacg ggttacggtg ttgaaaatcg ccccaacctg
     6661 accgtctttg atcatctcgc ggatcgcctc tttcggatta tccgggccta cgctgattaa
     6721 gcgcagctga ccaattttct catcaaccgt cattttcttg agcagatcgg tgacgaacgc
     6781 atcccgcgct tcaggcgtta atggatggtt accgaataaa tcatccgcca gtgctggctg
     6841 caggaccaga ctcaccgcga ttcctactga acatagccat ttcatatgga tttttcttcc
     6901 tttgttgccc gacgtggcag cgaaaatggt gcaaaaaccg tagtttgcca taagcatgat
     6961 ggagagagaa aaagaatgct cagtttattg tctgaatttt caaaatattc actcgctgaa
     7021 ttgtcataca aggcgctatt ctagtttgtg atattttttc gccaccacaa ggagtggaaa
     7081 atgtcttcca tgacaacaac tgataataaa gcctttttga atgaacttgc ccgtctggtc
     7141 ggtcattcac acctgctcac cgatcccgca aaaacggccc gctatcgcaa gggcttccgt
     7201 tctggtcagg gcgacgcgct ggctgtcgtt ttccctggct cactactgga attgtggcgg
     7261 gtgctgaaag cctgcgtcac cgccgacaaa attattctga tgcaggctgc caatacaggc
     7321 ctgaccgaag gatcgacgcc aaacggtaac gactatgatc gcgatatcgt gatcatcagc
     7381 accctgcgtc tcgacaagct gcacgttctc ggcaagggcg aacaggtgct ggcctatccg
     7441 ggcaccacgc tctattcgct ggaaaaagcc ctcaaaccgc tgggacgcga accgcattcg
     7501 gtgattggat catcgtgtat aggcgcatcg gtcatcggcg gtatttgtaa caactccggc
     7561 ggctcgctgg tgcaacgtgg cccggcgtat accgaaatgt cgttattcgc g
//
LOCUS       DAJTMX010000171         7542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTMX010000171 DAJTMX010000000
VERSION     DAJTMX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.171"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO145_003755"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO145_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO145_003756"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO145_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5468723.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO145_003757"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO145_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO145_003758"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO145_003758"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5468724.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO145_003759"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO145_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5468725.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO145_003760"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO145_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5468726.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO145_003761"
     CDS             complement(6510..7202)
                     /locus_tag="OO145_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5468727.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1832 c         1987 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccccccccgc gcgccgctga gaaaaagcga ag
//
LOCUS       DAJTMX010000172         7363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTMX010000172 DAJTMX010000000
VERSION     DAJTMX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.172"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            51..1541
                     /locus_tag="OO145_003762"
     CDS             51..1541
                     /locus_tag="OO145_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5468728.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO145_003763"
     CDS             1593..2750
                     /locus_tag="OO145_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5468729.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO145_003764"
     CDS             complement(2716..2838)
                     /locus_tag="OO145_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468730.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO145_003765"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO145_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7113
                     /locus_tag="OO145_003766"
                     /pseudo
     CDS             4764..7113
                     /locus_tag="OO145_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            7170..>7363
                     /locus_tag="OO145_003767"
     CDS             7170..>7363
                     /locus_tag="OO145_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468731.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
BASE COUNT         2162 a         1613 c         1562 g         2026 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgcaggccg tcgatgacaa aaacatgtac atgtattttt ttgaagagat gacggtctat
     4861 gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag catcgagcgt
     4921 ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc ggtacgcatg
     4981 gattcaacgc aaagtacctc gttgaaccag ggagatattc gccctgagaa aatctctatt
     5041 cacggtgcgt cgccctacca gaatgcctat ttgattgacg gtattagtgc aactaataac
     5101 ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg gatgtcacag
     5161 gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag ttttgtgccg
     5221 gttgaatttg gtcgcttcaa tggcggggta attgatgcaa agatcaaacg cttcaacgct
     5281 gatgatagca aggtgaaatt gggttatcgc actacgcgtt tggactggtt aacatcgcat
     5341 atcgatgaga ataacaagag cgcatttaat caaggttctt caggaagtac ctatttctct
     5401 ccagatttta aaaagaactt ttataccttg tcgtttaatc aggagctggc tgataacttt
     5461 ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga ttatgtttcg
     5521 aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac tgcattgagc
     5581 aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa atataccggc
     5641 tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat gggtaataaa
     5701 tcctatggtc tggcatggga tatggatacg caactcgcat gggccaaact acgtaccacc
     5761 gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg gtacaccgaa
     5821 ctttcatgta catatggtga tattacaggg cgttgtaccc gtggcggatt aggacacatt
     5881 tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa attcgccgtg
     5941 ggtgatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga tgcatggact
     6001 gaacgccata accagtctga atcctatgtg attaatgctg caggaaagaa aactaaccat
     6061 accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacgct gtatatggcg
     6121 gatcacatta gctggcggaa tgtgtcgtta atgcccggtg tgcgttatga ctatgacaac
     6181 tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat ttttgctgat
     6241 caaacctcaa tgattaccgc cggttataac cgttactatg gcgggaatat tcttgatatg
     6301 ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa aaccctgacg
     6361 cgttatcaga atttgaaaac gccttataac gatgaactgg caatgggatt gcagcaaaaa
     6421 atcgataaga acgttattgc acgcgcaagt gaagcgcatg atcaaatcag caaaagcagt
     6481 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6541 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6601 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6661 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6721 tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt aaacatggat
     6781 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6841 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6901 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6961 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     7021 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     7081 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     7141 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     7201 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag gatgaaaagt
     7261 taattaccgg gcaactggac aatgacttgc gatatatgat ttatccgcat gctcatccaa
     7321 aggatcaggt aaatttatgg ctgcaaattc ataccggttc att
//
LOCUS       DAJTMX010000173         7185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTMX010000173 DAJTMX010000000
VERSION     DAJTMX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.173"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="OO145_003768"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="OO145_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="OO145_003769"
                     /pseudo
     CDS             761..913
                     /locus_tag="OO145_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="OO145_003770"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="OO145_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5468732.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="OO145_003771"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="OO145_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4419..4538
                     /locus_tag="OO145_003772"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="OO145_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="OO145_003773"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="OO145_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5468733.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /gene="phoN2"
                     /locus_tag="OO145_003774"
     CDS             5983..6723
                     /gene="phoN2"
                     /locus_tag="OO145_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5468734.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7083..>7185)
                     /locus_tag="OO145_003775"
     CDS             complement(7083..>7185)
                     /locus_tag="OO145_003775"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5468735.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2250 a         1355 c         1385 g         2195 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgta ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact tttcataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc acactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggtgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagagaatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tattt
//
LOCUS       DAJTMX010000174         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTMX010000174 DAJTMX010000000
VERSION     DAJTMX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.174"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(296..1198)
                     /locus_tag="OO145_003776"
     CDS             complement(296..1198)
                     /locus_tag="OO145_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5468736.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO145_003777"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO145_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5468737.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO145_003778"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO145_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5468738.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO145_003779"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO145_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5468739.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO145_003780"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO145_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5468740.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNMFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO145_003781"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO145_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="OO145_003782"
     CDS             7090..>7183
                     /locus_tag="OO145_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5468741.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1677 a         1812 c         1875 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atatgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAJTMX010000175         7096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTMX010000175 DAJTMX010000000
VERSION     DAJTMX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.175"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..955)
                     /locus_tag="OO145_003783"
     CDS             complement(<1..955)
                     /locus_tag="OO145_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5468742.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAG"
     gene            1201..1827
                     /gene="gstB"
                     /locus_tag="OO145_003784"
     CDS             1201..1827
                     /gene="gstB"
                     /locus_tag="OO145_003784"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5468743.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1824..2938)
                     /gene="yliI"
                     /locus_tag="OO145_003785"
                     /pseudo
     CDS             complement(1824..2938)
                     /gene="yliI"
                     /locus_tag="OO145_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="OO145_003786"
     CDS             complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="OO145_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5468744.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3645..4970
                     /gene="rimO"
                     /locus_tag="OO145_003787"
     CDS             3645..4970
                     /gene="rimO"
                     /locus_tag="OO145_003787"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5468745.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5017..6345)
                     /locus_tag="OO145_003788"
     CDS             complement(5017..6345)
                     /locus_tag="OO145_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5468746.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6353..7090)
                     /locus_tag="OO145_003789"
                     /pseudo
     CDS             complement(6353..7090)
                     /locus_tag="OO145_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         1860 a         1815 c         1628 g         1793 t
ORIGIN      
        1 agcccgcttc gcctgccccg agattcactt cgatcttatc accaaaccag acgcgctgag
       61 tcacaaaggt ggcatcaggt ttaattggcg tcacagtttc aaagaagcgg aaaccccagg
      121 tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc agcactacgg
      181 agattaaacg catatcaccc tgggtagccg aagcaaccag attatatccc gcacctgccg
      241 tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga cggttacggt
      301 taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg tactcttccg
      361 gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta ctgaactgcc
      421 ccggcgcatc caggccgtgc accgtctgga aggtagtgtt ggtcagcccc agttttttgg
      481 cataaccatt catcagacca ataaaggact cctggctccc ggcaacgtaa tctgccagcg
      541 caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct gccaccgaaa
      601 cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga tttcccgtcg
      661 cccaggcatc tttaccgacc gtcaccatat cggtgagttt aatcttatcg gccttaagcg
      721 cctgcccaac cacatagctg gtcatgattt tagtcaggct cgcaggatcc agtttctcat
      781 ccgcattgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc catgcacgcg
      841 catccacgct cggcgcttca acggtttgtt cggccgcgaa tgccgttggg gcaaaaagga
      901 ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc gtcataaatg
      961 ccacccgagt atccattcaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta
     1021 ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa
     1081 tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa
     1141 ctttacttca ccatcggagt cctgttcagt ctgtggctgc ctgtcggaaa aggggtgagt
     1201 atgattacgc tgtgggggcg gaataattca actaacgtaa aaaaagtatt gctgacgctc
     1261 gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac
     1321 gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa
     1381 agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg
     1441 caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac
     1501 tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca
     1561 ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg
     1621 tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt
     1681 gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg
     1741 acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc
     1801 aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg
     1861 gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc
     1921 tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc
     1981 acaatgacat ctttatcttt cagctcccaa taaataattt ttgctgccac tgggggaatt
     2041 tatcgctgtt atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag
     2101 gttgctcggt cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt
     2161 tgattcccca ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt
     2221 catcaccacc gcgcgggcca tgttcattca gccacagtgc attactccac ggattcatcg
     2281 ccattccttg cggattacga atgccataag accagatctc ggcgcgcgca ccggattcct
     2341 ttataaaagg attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct
     2401 gtaatttatc cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa
     2461 gataaccttt accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg
     2521 gcatctggcg gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc
     2581 cataacccac agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga
     2641 tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt
     2701 gcgcccaaac gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt
     2761 gacgcaactc gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca
     2821 gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg
     2881 cccagagagc ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa
     2941 cgcctccgga ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt
     3001 tacaaaaact taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc
     3061 aagcatggcg tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc
     3121 aagcttatcg gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca
     3181 tactgcggct gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc
     3241 cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt
     3301 atctcgtaga tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac
     3361 gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct
     3421 gtcaacgaac atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct
     3481 attttttgag caaaatgctg tccaggatca attcttcacg ccgaattaac aaaatttaca
     3541 tttccttcgc ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag
     3601 aatccctgcc ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc
     3661 cccagccgaa aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc
     3721 gtattctcac cgaactccgc actgaaggtt atgacgtggt accgagctat gacgatgcga
     3781 acatggtgat cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag
     3841 ccattggtga agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa
     3901 aagaagatca gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct
     3961 atgagcaggt tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc
     4021 tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa
     4081 tttcggaagg ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc
     4141 tgatgagccg tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg
     4201 ttaaagagat tctggtgatc tcgcaggata cttccgccta tggcgttgat gttaaacatc
     4261 gtactggctt ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt
     4321 tatcgaaact ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg
     4381 acgtcatccc actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc
     4441 acgccagccc gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg
     4501 cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg
     4561 tcggcttccc tggcgagacg gaagaagatt tccagatgct actcgacttc ctgaaagaag
     4621 cacgtctgga tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg
     4681 ccctgcctga ccaggttccg gaagaagtga aagaagaacg ctggaaccgt ttcatgcagc
     4741 tgcagcagca gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga
     4801 ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa
     4861 tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg
     4921 tgaaagtcga gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg
     4981 ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgtgccct
     5041 gccgtttttc ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt
     5101 agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc ttcccacctg
     5161 gaaatgaacg atttctttta gtatcgatag caactcttcc agcgtgccgc cagtataaat
     5221 caccgcaaat tcgtcacctc cgaagcggta gactttacct ttatctaccg ggatctgtga
     5281 tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc
     5341 gttaatgttt ttgaatttat cgacgtcaaa cagacacaaa taaccgcctt tttccggcag
     5401 cgcttttaaa tcttcatcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa
     5461 cgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc
     5521 atccagtacg cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt
     5581 aatcactctg cgtaccagat agaggcaact ggcacccaat atcactgcca ccaatgcggc
     5641 aaagaaaata atcaatgtac aaacccgcaa ataaacatct tttttatacg caaatacgta
     5701 caatccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt
     5761 tttagcatta tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag
     5821 gttctcgctg tccatcacca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa
     5881 aaatatcaga ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc
     5941 aaaagagata aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc
     6001 acaataattt ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg
     6061 accgacatcg tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac
     6121 caacagctgt tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg
     6181 atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata
     6241 cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa
     6301 aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt
     6361 ttactaattt tccactttta tcccaagcgg agaatggcat gggtttctgg tacagatacc
     6421 cctgagcctg aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc
     6481 cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt
     6541 ttcccgcagt accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat
     6601 taatttcata aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc
     6661 gtaccccttg tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga
     6721 tcacggtttc agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga
     6781 tggtggtaaa acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat
     6841 gcagtaagaa atcatccttc agcgacgcac gttgactaaa atgtctgaat taacccattg
     6901 ccgttttaat aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa
     6961 tgaacttcag cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg
     7021 cgctcagaat ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc
     7081 caatttccat tgcatg
//
LOCUS       DAJTMX010000176         6963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTMX010000176 DAJTMX010000000
VERSION     DAJTMX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.176"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            256..1680
                     /gene="sbcB"
                     /locus_tag="OO145_003790"
     CDS             256..1680
                     /gene="sbcB"
                     /locus_tag="OO145_003790"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5468747.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1723..1950)
                     /locus_tag="OO145_003791"
     CDS             complement(1723..1950)
                     /locus_tag="OO145_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5468748.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1964..3022)
                     /gene="yeeE"
                     /locus_tag="OO145_003792"
     CDS             complement(1964..3022)
                     /gene="yeeE"
                     /locus_tag="OO145_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5468749.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3201..4559)
                     /gene="plaP"
                     /locus_tag="OO145_003793"
     CDS             complement(3201..4559)
                     /gene="plaP"
                     /locus_tag="OO145_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5468750.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4549..4611)
                     /gene="yoeI"
                     /locus_tag="OO145_003794"
     CDS             complement(4549..4611)
                     /gene="yoeI"
                     /locus_tag="OO145_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5468751.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4772..5755)
                     /locus_tag="OO145_003795"
     CDS             complement(4772..5755)
                     /locus_tag="OO145_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5468752.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5801..6625)
                     /locus_tag="OO145_003796"
     CDS             complement(5801..6625)
                     /locus_tag="OO145_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5468753.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6708..6962)
                     /gene="yoeB"
                     /locus_tag="OO145_003797"
     CDS             complement(6708..6962)
                     /gene="yoeB"
                     /locus_tag="OO145_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5468754.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1891 a         1816 c         1624 g         1632 t
ORIGIN      
        1 gcatcagcca cttcttgtgg gcccgaatat ggtttaccat aaatagtacg cagttttgtg
       61 tcgcttatag ccataaataa tatttggtac gctcagaaat aatagtttgg cacacatctg
      121 gtacacaata tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac
      181 atactcaggt gaaaaaaggg ttgcttttcg agttataaca gacagttaat taactacagt
      241 ggacaatgct agacaatgac agacacagat aaacaaccta ccttcctctt tcacgattac
      301 gaaacctttg gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc
      361 gatgacgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgcggatgac
      421 tatttacccc ggcctggagc agtattaatt accggtatta ccccgcagga ggcacgggcg
      481 aaaggagaaa acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag
      541 acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt
      601 tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat
      661 ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa
      721 aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggtattgaa
      781 catagcaacg cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg
      841 gtaaaaacgc gtcagccacg cctgtttgat tatctcttta cccatcgtaa taaacacaaa
      901 ctgatggcgt tgattgatgt tccgcagatg aaacccctgg ctcacgtttc cggaatgttt
      961 ggggcatggc gtggcaatac cagctgggtg gcaccgctgg cgtggcatcc tgaaaatcgc
     1021 aatgccgtaa ttatggcgga tttggcagga gacatttcgc cattactgga gctggatatc
     1081 gacacattgc gcgagcgttt atataccgca aaagccgatc ttggcgataa cgccgccgtt
     1141 ccggttaagc tggtgcatat caataaatgt ccggtactgg cccaggcgaa tacgctacgc
     1201 ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctaaaaatt
     1261 ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtagcga tattcgcgga agccgaaccg
     1321 tttacacctt cagataatgt ggatgcacag ctttataatg gctttttcag tgacgccgat
     1381 cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc
     1441 acttttgttg ataaacgaat tgaaaagttg ttgttcaatt atcgggcacg caacttcccg
     1501 gggacgctgg attatgccga gcagcaacgc tggctggagc accgccacca ggtcttcacg
     1561 ccagagtttt tgcagggtta tgctgatgaa ttgcagatgc tggtacaaca atatgccgat
     1621 gacaaagaga aagtggcgct gttaaaagca ctttggcagt acgcggaaga gatcgtctaa
     1681 tttgaagccg ccgccgctgg tacggcggag gacaatgttg agtcaggctt tttgaacggt
     1741 gatgctccac gctgcatcgc caatctgctg ataatcggtg atggcatgtc cttcttctgc
     1801 cgcccactgt gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc
     1861 gccgctggcc atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg
     1921 cgtcaccaca tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag
     1981 ttgccagtcg cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc
     2041 cgagaatcat aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca
     2101 gtccattacc gatagagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac
     2161 ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg
     2221 ctttggctgc aataaatgcc tccacgaaga tccctaacac caggaaaacg ccccagttaa
     2281 tgtatttcac gtcacccgcg accagaaatt gaagaatatt ggccgttggg gaagtgactc
     2341 ctaacccaaa catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac
     2401 cgatgagtac agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc
     2461 cggttcggcg cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca
     2521 ccacccagag cgttatcacc agcaaaacgg taaccaacgg ccacacagac aaattaaaag
     2581 tctcagcaat agagttatgt tcagtagtgt agtgctgcaa ggtttgattt aaaccactgg
     2641 cgtgtggaga acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc
     2701 cgatcaatcc ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa
     2761 ttcccagccc gaaaagatac ccacctataa cagtaccaag ccacgggaac gccccggctt
     2821 cgtaagtcag taggcccgcc tgaattaacg caaaaacccc cacgctttgt gccgaaattg
     2881 caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac
     2941 cagtcaggca aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc
     3001 cgcttaatat cattgaaaac ataaattaat taaccagatg aatgttaatg gggaaatttt
     3061 tcatgactgg tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt
     3121 aatgaggatt tcgataaaaa agaataaaaa aaccggagcc aaatgttcat tcgactccgg
     3181 tttattatta gaaaagatga ttatgctacg tcttcgtact gcggaaccgg attacggaag
     3241 ctcttggtga cgcaagccag gtaaatcaga ccgatagcag cccagatcag acccagaacc
     3301 attgagcttt cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc
     3361 aggaacagat actggaagtg atctttcagc gtcttgttac gcttctcacg gatccagaac
     3421 tgcgagatga ccgacaggtt aacaaaggtg aacgccacca gcgcaccaaa gttaatcagc
     3481 gccgttgcca ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg
     3541 atgttcatcg ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaac
     3601 acgccgtcac gacccattac gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg
     3661 gacgccagta cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca
     3721 acgtacagca tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg
     3781 aagtacagct gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc
     3841 aggaaaatcg cacgcgggat cacgcgctct gcatctttgg tttcttccga cagattgctg
     3901 atgccgtcaa agccggtaaa ggagaagcac aggattgtcg ccccggtaat catcgggata
     3961 acatgcgcat cgccagacca gaacggacgc gtgctcgcca gcgtaccagc gccttcacct
     4021 tcaaatacgc cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg
     4081 acgattacag tgttgacgtt cgctacggat ttcagactac gcaggttaaa ggcggtcata
     4141 aaggccacca gcgccaccac aaacatccac gatggaatgg aaggcaccag agcttcaaaa
     4201 tagattttcg ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac
     4261 caacccacca taaagccgac agtcgggcta atggatttct gggcgtaagt gtatgcagag
     4321 ccagcagaag gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca
     4381 atcaatgcga acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca
     4441 aatgtatcaa acagcgtcat cggctgcata taggctagac ccatcattac aaccggaact
     4501 aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg
     4561 gttattctcc tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga
     4621 ttcctcagcg acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc
     4681 tcttggtatg aatgattggt ttgaagcaaa aaataaccga cgctaattaa acgtcggttt
     4741 ttaatcattt tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg
     4801 agaggcttgt cataataatt tttctttgaa tggctgattt tccgtcatca gattttgcat
     4861 aaacaccggc gatcgcacta tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc
     4921 ctgcggtctg ttcattccag ccaatccaga caggttcaaa gagagtttgg gcgaccactt
     4981 tcttctcgat taacgcgcca ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc
     5041 cgcagccaag gccgctaatt tgcagctcca gtttggtttt aaaatcgaaa acggtaatgg
     5101 cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtatccccca
     5161 ccacaatcgc ccgatatcgt ttaatgatac gacggtttaa cggttcttct tcctgggcta
     5221 acggatgatg aggcgcgacc gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa
     5281 actcactgga agatggtggc tcatgcatcg cgccaacgat aatatccgct cgcccctgag
     5341 tcagggcatc ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat
     5401 gatgttgata aaaagcttca atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca
     5461 cgccaatcac cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt
     5521 ctcgcacggt atgcagaact tcccgccctt tctctaataa catttttccg gtgcgggtga
     5581 atttagcgcg gtgtccgcta cgatcaagca attgaatatt taggtcgctt tccagcttgt
     5641 gaacggtgta actcagagcg gacggcgtct tgtagagttt ggctgacgcc gccgcaaaac
     5701 tgccctcttt ttctatggca tcgaggatca tcaacacatc cagcagtggt ttcatacacg
     5761 cccccttgca gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccaac ggcattacca
     5821 gcggatcggg atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa
     5881 tcttaccttt gccgctgtcc agactatttc tgaactgcgg aggttgcagc cccagtaaac
     5941 gggccatctg cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga
     6001 tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat
     6061 ggactaaatt aacaccatgt tcaccatcag gggcggtttt tccggcaaag aagcgtcccg
     6121 gatgacgtcc cggtccgacc aggcccgcaa gacgcagaat atcgaccgaa gtaccgggta
     6181 aattgtgcag ccagtcttcg agttcttcta atactcgtcc actgttggtt accggattac
     6241 gcggggtagt ttctttaacc gtgccttgcg cgtcgccata gacagatgtt gagctggtaa
     6301 aaataattcg gggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta
     6361 aatagaactc atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg
     6421 catccataag ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca
     6481 gatagctatc aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc
     6541 cggtgacttg ccagcctcgc gctgaaagtg acatcgccag cggcatacct aaccacccta
     6601 acccgacaat tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc
     6661 tacgctagcg tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa
     6721 cgacatgctg caatgagcag tgaatcatca gtaaccgcgt ataccagacg gtgctcctct
     6781 gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca ggggttctgg tttcccctta
     6841 ccttcaaatg gtgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc
     6901 ttatctgttt cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc
     6961 act
//
LOCUS       DAJTMX010000177         6961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTMX010000177 DAJTMX010000000
VERSION     DAJTMX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.177"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(364..945)
                     /locus_tag="OO145_003798"
     CDS             complement(364..945)
                     /locus_tag="OO145_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468755.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(992..4849)
                     /gene="sigA"
                     /locus_tag="OO145_003799"
     CDS             complement(992..4849)
                     /gene="sigA"
                     /locus_tag="OO145_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5468756.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5210..6214)
                     /locus_tag="OO145_003800"
                     /pseudo
     CDS             complement(5210..6214)
                     /locus_tag="OO145_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6199..6576
                     /locus_tag="OO145_003801"
                     /pseudo
     CDS             6199..6576
                     /locus_tag="OO145_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6615..>6961)
                     /locus_tag="OO145_003802"
     CDS             complement(6615..>6961)
                     /locus_tag="OO145_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5468757.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1734 a         1567 c         1572 g         2088 t
ORIGIN      
        1 atcacctgct attaatcccc gagtcagtac ttcgcggcct gacgcgatca ccctactcag
       61 tacttcagag cccgcctccg gtacctgcct ggcgatcgcc cctgtgcaac aaaataaatc
      121 aaccgttatc aaaccacgat cgttcgtatg cgccgccgat gacgcgcgcc tgaaaaacgc
      181 gtcaatatga gttccccgga agcttacgat aatacgctta tgaacattac aacacgctca
      241 tgcggaacct ggatggggga aggaacggtg aagctgttag cgaatgaccg gcgtttatac
      301 aaacgccggt catgatgaat gatatttcag aatatacctc gggctaaaat accgggacag
      361 agatcaggcg tgattaatct gcagcatttt taataacaga agtactgagc ttactttacc
      421 tttgatgatg taatagttat ttccgtcggt actcattttt ccctgaaata atgtcacgga
      481 tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc atatttttaa tgagctgaga
      541 tccgggtggc aaggggtatt tgctgtcaga tactgggcga ctgagtctga tgtggcgcgt
      601 atacagcccg ttttcgtaca gggtggcctc tgttatggtg tcagtgacca gcatgtggga
      661 gacaatgctg tattcttttt taaagcaccc accagaaacg gtgattgtaa tgataatggt
      721 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      781 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      841 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      901 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctccggtaga
      961 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
     1021 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
     1081 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
     1141 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
     1201 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
     1261 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
     1321 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
     1381 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
     1441 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
     1501 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
     1561 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
     1621 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1681 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccaccagaag
     1741 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1801 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1861 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1921 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1981 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     2041 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     2101 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     2161 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     2221 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     2281 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     2341 ctgcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     2401 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     2461 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     2521 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     2581 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     2641 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2701 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2761 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2821 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2881 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2941 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     3001 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     3061 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     3121 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     3181 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     3241 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     3301 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     3361 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     3421 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     3481 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     3541 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     3601 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3661 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3721 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3781 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaataccc
     3841 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3901 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3961 ataacgagta gtttgtccag gctatattgg tactactgat aaaaccaact gtactggtaa
     4021 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     4081 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     4141 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     4201 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     4261 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     4321 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     4381 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     4441 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     4501 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     4561 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     4621 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4681 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4741 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4801 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4861 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4921 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4981 taacaaaatg aaaaaaaaga taagcacaag tgattgattg gttagatatt gatcacttgg
     5041 tgtttgtatt ttgaattttg cgatcaaaat ttgatctttg ttgtttccgg tcaatccttt
     5101 acaggttttc tgtattatcc gggggagtta agtctgtcga tgtccgacag acttttgatg
     5161 atggaaacaa catgcagcga taatgtgcat gctgttttgc aggtggaact cagaaggtga
     5221 actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc gatcaggcct gcaacatcaa
     5281 ggtgaaccac atcaaacggt gagatcccca ccccggccgt gaatgcactg ccgtcgttac
     5341 cggccagatt ctgacggtaa ccggcgcgca gctgtgccca tttccaggca ttaaattctg
     5401 tgccaatggc ggcaaactga cggttgctgt cggaggtgaa accgctggcc ggggtcagat
     5461 ccacatcaaa tgcagtggtg aacatcgcgt tgtgccagga gacaccggcc gtcacctgcg
     5521 ggcggatcct gaaggtttcc gtgataccgt tcacttcttt tgtctcaata ctacgggaga
     5581 tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc caggtccgta ctggcaccga
     5641 tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc gcctttgaag gcgctgctgt
     5701 cataatttct gaccagcacg ttgtagttga acaggtcgac gcgctggaat ttggggggga
     5761 agcccagaga ccatttctgc ccggccgttt ccagttccgt agccagagca atacccacat
     5821 cagtgatcag tgccgtgcgg gcacaggcct gtgatgtcag gcggcttttg tccacctcct
     5881 gcccggtgct gtttgccacc tcttccaggt aagtgaggtc agcatcagag actttaccgt
     5941 cgacactgac ggtgccgtgt gatttaacca tcagtgcggc agagaggcga tcaccgggaa
     6001 gggccgccac agcagaaacg cccagctgtg cggcggcatg tgtgttacgg aagtcccgga
     6061 ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg gttactttcg atggcgcggt
     6121 cgaaggcctt ccactcatca ctgatccggt cggcgttgtc ggtgatatta tccggattgg
     6181 agagctgcgc gccgacggac ggaagcagga tgctgggctc atgcgcgacg caaaatccac
     6241 gatgtatata tcagtactaa aagcgcgacg gcggaagaag ctctgaaact aatcggtgaa
     6301 ctgtacgcca tagagcacga tatacgcggg ttaccggtgt ctgaacgcct ggcggtcagg
     6361 caaatgcaga gtagaccgct cctgacttcc ctgtataagc tgatgcagga gaaagaacac
     6421 acgttatcga agaaatgtcg tctgggagat gcgttccggt atatcaggaa gcactgggct
     6481 gcgttgtgca acttctgtga tgatggcctg gctgaggtag ataataatgc agcagaaaga
     6541 gcgcttcgtg cagtctgtct cgggaagaaa actttttcga ggatcactca ttccgtgtct
     6601 gactgcttca gggtttaatt ttgcttctgc tgactgaatg atgattgggc agcaagtgca
     6661 atatcccaca taaatccagt caactcacga gccaccgccg taatggctac ttgtgatttt
     6721 ttcccttttg ctgtcagagt tcggtaacga gaacacaatc gttgttgagc tttccatgca
     6781 atatctttgg aatattgtgt tacagagtcc ggtcggtaat atataagcca tgctccaact
     6841 tttgcaggtg tacgataaga ccaggcggct tcgtaaagca gacgtctcag ttcgctgttt
     6901 ccaacttttg ttattcctct gggacgaata ctgtttccac tggaatattc acctggcacc
     6961 a
//
LOCUS       DAJTMX010000178         6910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTMX010000178 DAJTMX010000000
VERSION     DAJTMX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.178"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..694
                     /locus_tag="OO145_003803"
     CDS             <1..694
                     /locus_tag="OO145_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5468758.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="OO145_003804"
     CDS             complement(1033..1803)
                     /locus_tag="OO145_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468759.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="OO145_003805"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="OO145_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="OO145_003806"
     CDS             complement(2294..2515)
                     /locus_tag="OO145_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468760.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="OO145_003807"
     CDS             complement(2516..3199)
                     /locus_tag="OO145_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5468761.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="OO145_003808"
     CDS             complement(3263..3574)
                     /locus_tag="OO145_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468762.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="OO145_003809"
     CDS             complement(3571..4473)
                     /locus_tag="OO145_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5468763.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="OO145_003810"
     CDS             4913..5872
                     /locus_tag="OO145_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5468764.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="OO145_003811"
     CDS             5872..6267
                     /locus_tag="OO145_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5468765.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="OO145_003812"
     CDS             6450..6830
                     /locus_tag="OO145_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5468766.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1887 a         1786 c         1676 g         1561 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat gacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac
//
LOCUS       DAJTMX010000179         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTMX010000179 DAJTMX010000000
VERSION     DAJTMX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.179"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            240..2672
                     /locus_tag="OO145_003813"
     CDS             240..2672
                     /locus_tag="OO145_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5468767.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO145_003814"
     CDS             2739..4208
                     /locus_tag="OO145_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5468768.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO145_003815"
     CDS             4208..5869
                     /locus_tag="OO145_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5468769.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO145_003816"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO145_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5468770.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO145_003817"
     CDS             complement(6478..>6795)
                     /locus_tag="OO145_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468771.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTMX010000180         6772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTMX010000180 DAJTMX010000000
VERSION     DAJTMX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.180"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..150
                     /gene="rpsH"
                     /locus_tag="OO145_003818"
     CDS             <1..150
                     /gene="rpsH"
                     /locus_tag="OO145_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5468772.1"
                     /translation="GLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEI
                     ICYVA"
     gene            163..696
                     /gene="rplF"
                     /locus_tag="OO145_003819"
     CDS             163..696
                     /gene="rplF"
                     /locus_tag="OO145_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5468773.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            706..1059
                     /gene="rplR"
                     /locus_tag="OO145_003820"
     CDS             706..1059
                     /gene="rplR"
                     /locus_tag="OO145_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5468774.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            1074..1577
                     /gene="rpsE"
                     /locus_tag="OO145_003821"
     CDS             1074..1577
                     /gene="rpsE"
                     /locus_tag="OO145_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5468775.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGIHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            1581..1760
                     /gene="rpmD"
                     /locus_tag="OO145_003822"
     CDS             1581..1760
                     /gene="rpmD"
                     /locus_tag="OO145_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5468776.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            1764..2198
                     /gene="rplO"
                     /locus_tag="OO145_003823"
     CDS             1764..2198
                     /gene="rplO"
                     /locus_tag="OO145_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5468777.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            2206..3537
                     /gene="secY"
                     /locus_tag="OO145_003824"
     CDS             2206..3537
                     /gene="secY"
                     /locus_tag="OO145_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5468778.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            3569..3685
                     /gene="rpmJ"
                     /locus_tag="OO145_003825"
     CDS             3569..3685
                     /gene="rpmJ"
                     /locus_tag="OO145_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5468779.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            3832..4188
                     /gene="rpsM"
                     /locus_tag="OO145_003826"
     CDS             3832..4188
                     /gene="rpsM"
                     /locus_tag="OO145_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5468780.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            4205..4594
                     /gene="rpsK"
                     /locus_tag="OO145_003827"
     CDS             4205..4594
                     /gene="rpsK"
                     /locus_tag="OO145_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5468781.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            4628..5248
                     /gene="rpsD"
                     /locus_tag="OO145_003828"
     CDS             4628..5248
                     /gene="rpsD"
                     /locus_tag="OO145_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5468782.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            5274..6263
                     /gene="rpoA"
                     /locus_tag="OO145_003829"
     CDS             5274..6263
                     /gene="rpoA"
                     /locus_tag="OO145_003829"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5468783.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            6304..6687
                     /gene="rplQ"
                     /locus_tag="OO145_003830"
     CDS             6304..6687
                     /gene="rplQ"
                     /locus_tag="OO145_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5468784.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         1632 a         1596 c         1842 g         1702 t
ORIGIN      
        1 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
       61 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
      121 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
      181 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
      241 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
      301 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
      361 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
      421 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct
      481 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
      541 actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg
      601 gatctgcgcg cctaccgtcg tcctgagcct tacaaaggca agggggttcg ttacgccgac
      661 gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
      721 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
      781 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
      841 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
      901 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
      961 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
     1021 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
     1081 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
     1141 aaaccgttaa aggtggtcgt atattctcct tcacagctct gactgtagtt ggcgatggta
     1201 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
     1261 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctacaac
     1321 accctgttaa aggtattcac acgggttctc gcgtattcat gcagccggct tccgaaggta
     1381 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
     1441 ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg
     1501 gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
     1561 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
     1621 tcggtcgtct gccgaaacac aaggcaacgc tgcttggctt gggtctgcgt cgtattggtc
     1681 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
     1741 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
     1801 aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
     1861 gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
     1921 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
     1981 ttacagccga agttcgtcta tctgacctgg ctaaagtaga aggcggtgtt gtagacctga
     2041 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
     2101 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
     2161 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
     2221 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
     2281 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
     2341 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac
     2401 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat
     2461 atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt ggcagaaatt
     2521 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgtta cggtactctg
     2581 gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg
     2641 caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc
     2701 acaggaacga tgttcctgat gtggttgggc gaacagatca ctgaacgagg tatcggcaac
     2761 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
     2821 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
     2881 ttagtatttg cagtgacgtt ctttgttgtg tttgttgagc gtggtcaacg ccgcattgtg
     2941 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
     3001 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
     3061 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
     3121 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
     3181 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
     3241 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
     3301 atcgataaag taatgacccg cctgaccttg gttggtgcgc tgtatattac ctttatctgc
     3361 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
     3421 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
     3481 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
     3541 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg
     3601 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
     3661 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
     3721 gctggctaga ttagccagcc aatcttttgt atgtctgtac gtttccattt gagtatcctg
     3781 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
     3841 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
     3901 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
     3961 aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt
     4021 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
     4081 ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
     4141 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
     4201 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
     4261 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
     4321 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
     4381 tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat
     4441 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
     4501 tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa
     4561 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag
     4621 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
     4681 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
     4741 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
     4801 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
     4861 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
     4921 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
     4981 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
     5041 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
     5101 tctggagctg gctgagcagc gtgaaaagcc aacttggctg gaagttgatg ctggcaagat
     5161 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
     5221 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
     5281 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
     5341 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
     5401 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
     5461 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
     5521 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
     5581 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgttgaaa
     5641 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
     5701 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
     5761 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
     5821 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca
     5881 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
     5941 ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga
     6001 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
     6061 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
     6121 agcgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
     6181 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcttggaa aactggccac
     6241 cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag
     6301 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
     6361 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
     6421 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
     6481 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
     6541 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
     6601 tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca
     6661 gagaaagcag aagctgctgc agagtaatct gaagcaatgt aaaaaaaccc gccccggcgg
     6721 gtttttttat acccgtagta tccccactta tctacaatag ctgtactctt tt
//
LOCUS       DAJTMX010000181         6586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTMX010000181 DAJTMX010000000
VERSION     DAJTMX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.181"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            17..1075
                     /locus_tag="OO145_003831"
     CDS             17..1075
                     /locus_tag="OO145_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5468785.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGCIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1118..1621
                     /gene="yeaK"
                     /locus_tag="OO145_003832"
     CDS             1118..1621
                     /gene="yeaK"
                     /locus_tag="OO145_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5468786.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1622..1726)
                     /locus_tag="OO145_003833"
     CDS             complement(1622..1726)
                     /locus_tag="OO145_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468787.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1896..2342
                     /locus_tag="OO145_003834"
     CDS             1896..2342
                     /locus_tag="OO145_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5468788.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="OO145_003835"
                     /pseudo
     CDS             complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="OO145_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3214..4394
                     /gene="nimT"
                     /locus_tag="OO145_003836"
                     /pseudo
     CDS             3214..4394
                     /gene="nimT"
                     /locus_tag="OO145_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4449..4796
                     /locus_tag="OO145_003837"
     CDS             4449..4796
                     /locus_tag="OO145_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5468789.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="OO145_003838"
     CDS             complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="OO145_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5468790.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5255..6280
                     /gene="dgcP"
                     /locus_tag="OO145_003839"
     CDS             5255..6280
                     /gene="dgcP"
                     /locus_tag="OO145_003839"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5468791.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6313..6411
                     /locus_tag="OO145_003840"
     CDS             6313..6411
                     /locus_tag="OO145_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5468792.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6414..6488
                     /gene="yoaJ"
                     /locus_tag="OO145_003841"
     CDS             6414..6488
                     /gene="yoaJ"
                     /locus_tag="OO145_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5468793.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1594 a         1594 c         1613 g         1785 t
ORIGIN      
        1 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
       61 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
      121 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
      181 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
      241 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
      301 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
      361 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
      421 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
      481 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
      541 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
      601 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
      661 cgtccatgac ttacctggct gcattcgtgt ctcgttatcc attgatattc tttactttat
      721 cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
      781 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
      841 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
      901 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
      961 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa
     1021 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     1081 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     1141 cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     1201 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg
     1261 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     1321 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     1381 attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg
     1441 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     1501 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     1561 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     1621 gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa
     1681 acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg
     1741 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     1801 tgatttttta tgcaaaaaac gtaaagattt tttacttcct tcctgcagca agcataaagt
     1861 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     1921 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     1981 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     2041 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     2101 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     2161 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     2221 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     2281 gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt
     2341 agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat
     2401 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     2461 cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa
     2521 ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     2581 ccattgcccc aatgcccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg
     2641 gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc
     2701 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     2761 gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt
     3121 gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     3181 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     3241 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     3301 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     3361 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct
     3421 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     3481 tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     3541 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     3601 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     3661 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     3721 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     3781 agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt
     3841 tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg
     3901 attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt
     3961 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc
     4021 ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     4081 ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     4141 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg
     4201 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     4261 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     4321 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     4381 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa
     4441 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     4501 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     4561 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     4621 tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag
     4681 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     4741 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     4801 agtacggcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     4861 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     4921 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     4981 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     5041 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccaagct
     5101 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     5161 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     5221 tcagcactta cccgccgtat cctggagttg ttccgtgtca gatcagatta tcgcccgcgt
     5281 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat
     5341 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     5401 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     5461 cgtcttgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgct ttttcagcga
     5521 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt
     5581 tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     5641 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     5701 gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcacgaag ccaatgctgc
     5761 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     5821 tgaaaatctg acgacgctgt tttccctcgc ccggcatctt aaccataaga taatgatcgc
     5881 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     5941 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     6001 tggtcgtctc ggtggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat
     6061 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     6121 tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga
     6181 tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca
     6241 gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg
     6301 attggtgata acatgcgaat cggtattatt tttccggttg taatcttcat tacagcggtc
     6361 gtatttttag catggttttt tattggctgc tatgctgccc cgggagcata aagatgaaaa
     6421 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     6481 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     6541 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatc
//
LOCUS       DAJTMX010000182         6547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTMX010000182 DAJTMX010000000
VERSION     DAJTMX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.182"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            7..657
                     /locus_tag="OO145_003842"
     CDS             7..657
                     /locus_tag="OO145_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468794.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO145_003843"
     CDS             629..1063
                     /locus_tag="OO145_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5468795.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2648)
                     /locus_tag="OO145_003844"
     CDS             complement(1836..2648)
                     /locus_tag="OO145_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5468796.1"
                     /translation="MIPYFTFAFSILIINTINSGETIDYIYNHIYDIIYGVRNNQFVG
                     TIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFMLSQTVLNHNPLLDPQWFWNID
                     SAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITAIMSAYQLLNQKPLLFKIIS
                     IFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYIT
                     KLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKIGHWLSRTVGGPFLIK"
     gene            complement(2648..2884)
                     /locus_tag="OO145_003845"
     CDS             complement(2648..2884)
                     /locus_tag="OO145_003845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468797.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFLLSKKMTYLFSIISSQSFTD"
     gene            3335..4525
                     /locus_tag="OO145_003846"
     CDS             3335..4525
                     /locus_tag="OO145_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5468798.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGSARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4915..6375)
                     /gene="gtrII"
                     /locus_tag="OO145_003847"
     CDS             complement(4915..6375)
                     /gene="gtrII"
                     /locus_tag="OO145_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5468799.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6372..>6547)
                     /locus_tag="OO145_003848"
     CDS             complement(6372..>6547)
                     /locus_tag="OO145_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5468800.1"
                     /translation="VFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNR
                     PKYIIKKSHRGNP"
BASE COUNT         2050 a         1191 c         1325 g         1981 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaccagca gcgaaaaaga atagtgggac gtgataacta aaaacaaaag
     2761 gatacaattt gccagccccc aacccaaggt gacctaaata tatagcaaaa ataccgagaa
     2821 attttaaagc atcaacccaa tcatgtctta tttttctgtt gctgtcgatt ttgttattat
     2881 acatttaaag taggctctcc agttttgtgt gatttttaaa cactcattgc aaatatgaat
     2941 gcctgaaagt ttatgattat taatcaacga gttcaatatt aatttattat ttttcaaagt
     3001 cgcactatat aggcatttac ttccgcagta ccaccgatag tatcgatatc aaatcagcct
     3061 gcggccgcga taactttcgt aacattctgc cagcggcttt gttgaataaa tcaggttcaa
     3121 gataatcgtc tctgcatcag gttagagttt caggcaatac atcctctttc tggcatacct
     3181 gccactctcg caccatggtc atagccttta cgatctgcat actcaccaga ccagagcccg
     3241 ctccctttta ggctgaggaa tccccagaaa agaagacagg catagagttt tgtgatctac
     3301 tgattgcgct aacaaattca atgagatcac ctctatgcct gcccgtaaag tatgccagac
     3361 ttttttccgt aatgctttag cttccacgca tcagtatcga caaaatgcca ttattgattc
     3421 tgctgctgct ttagtcggtg gcgcatctct ttctcttacc agcattggcc ggcatttacc
     3481 cggttctgct cgcgtgaaag ataaaataaa acgcgttgac tgtctgttgg gtaataaacg
     3541 acttcataat gatatccctc tgatattcaa aaatattaca tccatgatga caaataaact
     3601 gtcgtggtgt gttatcgctg ttgactggag cggttaccct tttcaggaat atcatgtcct
     3661 tcgtgccagc ctcctctgcg acggtcgctc tatccccctg atgagtcagg tcttcccgtc
     3721 aaaaaaaaat aacgaggcgg tggaaatcgc ttttctggat gcgctttccg gggccatttc
     3781 tccccggact cgtgttgtta ttgttactga tgctggtttt caaagtgcat ggttccgtca
     3841 tatcaaatca cagggatggg attttattgg tcgtatcagg ggggtagtga agttcaggct
     3901 ggatagtgac aaagataagt ggctggacat aaaaatgtgc agggggagtt cagaggcaaa
     3961 atacctgggg acgggaaccc tggcccggaa aaaacgctcg cagtgtgaag gacattttta
     4021 tctttataaa cattcaccta aaggcagaaa aagcaggcgt gccagaggga ggccgggctt
     4081 accaacaacg gaaaaagaac aaaaagccgc aggcagagaa ccatggttaa tattcagcaa
     4141 tacagctgag tttaatgcca aaaaaatcat gaaactgtac agtcgaagaa tgcagataga
     4201 gcaaaacttt cgggatgaaa aaagtgaacg ctttggtttt ggccttcggg cgagcagaag
     4261 tcggagggga gagcgttttc tggtattaag tctactggtt acactggcca gtatcgtact
     4321 ctggctactt ggttatcatt ttgaaaataa aggatttcat ttaaagtatc aggctaatag
     4381 tctcaaaaaa cgaagagtat tgtcttttct gacgctggct gaaaacgtcc tgagatttaa
     4441 ccctgaactt ctacgacgag cacaacctga aaagatattg tgccggttgg ccagcactta
     4501 ccgaagtatg gtgctggctt actgatgaaa attttctggg gatccctcag cctttcgggg
     4561 aggaatgagg gctctgatat tcttttgccg tagttcatcg tgaaaccgtc ggatgtcatt
     4621 tggcctgagt gacccatgcc tcccaaaagc agagtcgcta gtgttatgta attaggcggt
     4681 taaacttcaa ttctcagtgt taagtttggg taaacgcttg taaatggtat tgctaatttt
     4741 ttaattgact aatattagaa acggttagtg tgattgtata tagatatttg agtgtaaggt
     4801 atttaaaggg gcattcagcc cctccgtcta tttgtgattg catccattga tgtgtgtatt
     4861 attttgagct tgaaaaaggg aggcgctctt taataataaa taaatctatc aaagctaaat
     4921 attaaatgga agccaccctt ttttctctga tagttttatt atcaccaaac catttatttc
     4981 agctatcgaa tctggattcg gccaggaagg taaactcttc acaattggat aaatcttctc
     5041 tatttgttct ttatttgctg gtttgcaatt acagattcca tagtaattca taaatgcaac
     5101 catcctaaaa tagttgccac catcccacca aaaaaaagat tttccaaaag tgtctgcgct
     5161 catggagagc atatttcctg agtcgtttga accatatata tatatatatt ttccgttaag
     5221 ggaaatgcct tttgtttgag atgtgtatga tatttcttta gctaaagaga tatctttctg
     5281 tcttgcgaga gtatcattca aaaatagatt tttggatgtt gaaacgccat taaatataat
     5341 aaataaaaaa aacatgcagt ttaggtattt tatagagcga aaattgctta agcaagaaaa
     5401 caaaattact gctactatag gcattaaaac aaacaggcga ggtggcgccc ctgagccaac
     5461 aacaacgata aatataaccg gaagtatata aatgaaaatg attgatgaaa tcaaataaat
     5521 tgatcttgtt tttaatttat atgttagggt aaacatcaga attaataaag gaatcagtaa
     5581 ccacttgtat aggtttaaac cagtgggagg attgttatag aagttatata tattatctat
     5641 ggctgattta acaagccatt taatatcgct tgcattttgt atataatttg agatgtactc
     5701 gttcgattca agcgaaaaat aatgcttgat agctttggtt aatagcaggt aaattaaggt
     5761 agataaagct atgagggata gagataaaca tgaactataa aaagattcac gaatattttt
     5821 ctcatttcgt attatcgaat ttatctgcaa cccaatgacg aatgcaataa tatatgttac
     5881 gaatgtttgg tagattgcca ttgaaagaat ggacagtact ataccagaaa atatcacaat
     5941 cctatttttt tgcgagtgaa aaataattgc tgatatcgct gccagtagaa aagcaattcc
     6001 cacagtatca gcttggttaa gaaactctaa ttgataggag atctgaggga aggtaataaa
     6061 tactaacata cctatcaata attcataaga ttctagcttt agcgatctgc agattataaa
     6121 tgctgaaatg atgataaaag ataaggctat taatggtgtt atataaagtg aaaaaggctc
     6181 agggagaaag taatgtcgca aaaaagtgtg aaaccaccgc ccaagcgtga ttgtttgata
     6241 aaaattattt gtaaactcgc catcaatatt aagtgttcca tcgtacaacc acccccaata
     6301 acaataaatt gatattgcaa aacatgatat gaaggcaagg agatttgcat ttttaaataa
     6361 attaatttta atcatgggtt acctcgatga gattttttta tgatgtattt gggtctattt
     6421 tttacttcaa tatagattct acctatatat tctccgagaa caccaatccc gatcagttgc
     6481 actccaccca agaaaagtat tgatacaagc agggagggat acccgcgtac tgggttacca
     6541 aagacaa
//
LOCUS       DAJTMX010000183         6519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTMX010000183 DAJTMX010000000
VERSION     DAJTMX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.183"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO145_003849"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO145_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5468801.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO145_003850"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO145_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..6519
                     /gene="narH"
                     /locus_tag="OO145_003851"
                     /pseudo
     CDS             5646..6519
                     /gene="narH"
                     /locus_tag="OO145_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
BASE COUNT         1557 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     5881 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     5941 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     6001 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     6061 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     6121 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     6181 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     6241 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     6301 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     6361 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     6421 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     6481 catcgaggaa gcgtcgagca ccgagcgcga agttgacct
//
LOCUS       DAJTMX010000184         6321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTMX010000184 DAJTMX010000000
VERSION     DAJTMX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.184"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            73..378
                     /locus_tag="OO145_003852"
     CDS             73..378
                     /locus_tag="OO145_003852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5468802.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO145_003853"
     CDS             complement(454..918)
                     /locus_tag="OO145_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5468803.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO145_003854"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO145_003854"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5468804.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO145_003855"
     CDS             complement(1787..2476)
                     /locus_tag="OO145_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5468805.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO145_003856"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO145_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5468806.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO145_003857"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO145_003857"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5468807.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO145_003858"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO145_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5468808.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1610 a         1709 c         1564 g         1438 t
ORIGIN      
        1 atcatcagat gactgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAJTMX010000185         6253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTMX010000185 DAJTMX010000000
VERSION     DAJTMX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.185"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO145_003859"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO145_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5468809.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO145_003860"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO145_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5468810.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO145_003861"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO145_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO145_003862"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO145_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1658 a         1458 c         1476 g         1661 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atg
//
LOCUS       DAJTMX010000186         6162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTMX010000186 DAJTMX010000000
VERSION     DAJTMX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.186"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..171
                     /locus_tag="OO145_003863"
     CDS             <1..171
                     /locus_tag="OO145_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5468811.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO145_003864"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO145_003864"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5468812.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO145_003865"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO145_003865"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5468813.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO145_003866"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO145_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5468814.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO145_003867"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO145_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5468815.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6162
                     /locus_tag="OO145_003868"
                     /pseudo
     CDS             5430..6162
                     /locus_tag="OO145_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1581 a         1310 c         1558 g         1713 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatat
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac gatctgttta aa
//
LOCUS       DAJTMX010000187         6129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTMX010000187 DAJTMX010000000
VERSION     DAJTMX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.187"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..361)
                     /locus_tag="OO145_003869"
                     /pseudo
     CDS             complement(1..361)
                     /locus_tag="OO145_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="OO145_003870"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="OO145_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="OO145_003871"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="OO145_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="OO145_003872"
     CDS             complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="OO145_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5468816.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="OO145_003873"
     CDS             complement(2629..2943)
                     /locus_tag="OO145_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468817.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="OO145_003874"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="OO145_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="OO145_003875"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="OO145_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5468818.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4504..4641)
                     /locus_tag="OO145_003876"
                     /pseudo
     CDS             complement(4504..4641)
                     /locus_tag="OO145_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4723..5021)
                     /locus_tag="OO145_003877"
                     /pseudo
     CDS             complement(4723..5021)
                     /locus_tag="OO145_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5610..5769)
                     /locus_tag="OO145_003878"
                     /pseudo
     CDS             complement(5610..5769)
                     /locus_tag="OO145_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5773..6129)
                     /locus_tag="OO145_003879"
                     /pseudo
     CDS             complement(5773..6129)
                     /locus_tag="OO145_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1654 a         1352 c         1239 g         1884 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt
      181 tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag
      241 tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct
      301 gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca
      361 tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgccctcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gatcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcca
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg ataaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc
     3001 cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag
     3241 tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attatttttc attcctttat tttcattagc agtattgctg cctattatta
     4081 agtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa acaataacct atttttgtaa tgtaatagat attagtctat
     4201 tgctaatcta aaaaaaccat tgccacattg aatggctata ttcaccaata aattctttcg
     4261 atttcgtata ttgacatatc gtagatatat cttatcccta agattcaata atcgagtccg
     4321 tttaccattc tgtgcaagcg ccttcactca gaagtgaccg tcaaggcgat caccaaactt
     4381 gataataaag cagctcattg ccatacgcca gtccttaagg ggcgttgtcc atttctgtga
     4441 tgctgcctag atttaactgc cagctccggc ttatctacat ctaaatatgt gagcctaata
     4501 ccgctacact ttgtccagac tatgtttttc tccgggaagt gggctaaccg tctccataaa
     4561 gtgacgaact tttttcaaca gtggccatat tgaacggcac tgatgattac gggttattgt
     4621 ctcatgaaga gccggccaca tcactgacag cagtgacttc aggccttcag caccagacag
     4681 cctgaaccag ttaatccatc gcccgacaaa tttagtaatc tagggctggt ttttcttcag
     4741 ctgttgcggt tcgaaagtgc cattacgatc acgcagagta cggatttcca gaggttcatc
     4801 tccagtagtg ataatcttcg ttaagcaacc attgcgggaa ttgctgccag atctaagctg
     4861 atttttctcg cacccaagat gatgagtcat attagcattg agtacggttt cattttcagc
     4921 atccaatcaa actgactgag atcttcagga tttttgagac ttttggctag ttcattagct
     4981 aaagcccgca acttattaga tgtcaactgt ttttcattca taaattcacc ttcatttgat
     5041 atcaaattaa atagttcaac atcagagaat tatacaaact tatgtacagg ctcctatgat
     5101 cttcgaatcc tgaagattat agtgaaaata agttttcacg ttgaaatccc acatagttag
     5161 tatatgataa ataatcatgg atgacctgat taacctcttg gggtagtgct tttatagaag
     5221 aataaatatt ctactaattt aatacatcac ctaaaatagt attgtttttt atggttggtt
     5281 gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc cagtgccaga tcttacttat
     5341 aataaagggg cttggcagtt tggataaact attgttctta ttccactaat acaaactttt
     5401 tgtaaagtat attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa
     5461 caattacacc ataaaaggaa gagggggccc taattaagtt gtcaagcatg tttatgatac
     5521 ctgcagggta taaattttct ggctgcaagc agacacatat aaaacgtctt tattgatcct
     5581 acccacataa tatggacaca gtcgtaagtg aggctctggt tttcaaattg ttccggactg
     5641 agaccaccac accaacggtg ccgtcgccag cgattgtaat cgcactcgat ataactaaac
     5701 actgttgccc gcactatttc ccgatggata atagtgttct ccgtggagac attccatttt
     5761 cagcgaatga attttcatct gttgctgtat ccagtcatac agtgactgca tcagtggcgc
     5821 ggctctggct tttcttgccg ccagacgctg ttctgctgaa catccccgga cttctgcctc
     5881 gatggcatac agttcaccga tacgctgcag ggcttccgtg gtgatgtcgg ttggcactct
     5941 tgcatgcaca tcgtggattt ttctccggac atgagccata catgcggctt ccgttattct
     6001 gccggattcg tacaacgccc ggtaaccacc gtaagcatcg gcctgaagca ctccgctgta
     6061 accggccaga tgattctgtg gatggatacc tttccggtcc ggtgagtacg cgaaccagac
     6121 cgccggagg
//
LOCUS       DAJTMX010000188         6073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTMX010000188 DAJTMX010000000
VERSION     DAJTMX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.188"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..158)
                     /locus_tag="OO145_003880"
     CDS             complement(<1..158)
                     /locus_tag="OO145_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5468819.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLDLP"
     gene            394..666
                     /locus_tag="OO145_003881"
     CDS             394..666
                     /locus_tag="OO145_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5468820.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(632..1039)
                     /gene="nudG"
                     /locus_tag="OO145_003882"
     CDS             complement(632..1039)
                     /gene="nudG"
                     /locus_tag="OO145_003882"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5468821.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1126..1746
                     /locus_tag="OO145_003883"
     CDS             1126..1746
                     /locus_tag="OO145_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5468822.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1755..3062)
                     /gene="ynjE"
                     /locus_tag="OO145_003884"
                     /pseudo
     CDS             complement(1755..3062)
                     /gene="ynjE"
                     /locus_tag="OO145_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3129..3782)
                     /locus_tag="OO145_003885"
     CDS             complement(3129..3782)
                     /locus_tag="OO145_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5468823.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3782..5253)
                     /locus_tag="OO145_003886"
                     /pseudo
     CDS             complement(3782..5253)
                     /locus_tag="OO145_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5226..6073)
                     /locus_tag="OO145_003887"
                     /pseudo
     CDS             complement(5226..6073)
                     /locus_tag="OO145_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1434 a         1625 c         1601 g         1413 t
ORIGIN      
        1 ggcaaatcca atgacatctg gcgatatttt ggattttgtt caagaaaagg ccagagtgtg
       61 gtcattactt cacgaacggc ttgcgcgaac tcggtttgat tcgggtcgcg cttttggaca
      121 tggttgagga atgactccag agaatatgtc tgatccatag atataaaaac cttatatatt
      181 aatacgattg tgcttatgtt gtttatgtca tgtgcttttg cagttttcga ctataccatc
      241 aagaatgtga cggtaagcaa gaaaaaaatg acccaggaaa gcaaattaat aacgagagta
      301 atctcataac caaatgcgat aactcagacg ctaaagtttt gccacccggc taccgttatc
      361 agtagcagag acaataacgt taaggagttc cctgtgagtc gagcattgtt cgccgtagta
      421 ttagcatttc cattaattgc actggctaac ccgcattatc gtccggacgt ggaggtaaac
      481 gtaccgccag aagtgttcag ttcaggcggg cagtcagcgc agccctgtac tcagtgctgc
      541 gtttatcagg atcaaaatta ttctgaaggg gcggtaatta aagccgaagg aatactgctg
      601 caatgccagc gtgatgacaa aacccttagc actaatccgc tgctctggcg gcgcgtaaag
      661 ccataaacgc ctctaataat ggaatgtcag caggggccag cggatattgc agcgcctctt
      721 caggtgagca ccagaccagc gcctgatgtt catgtgcctg taacgtcccg tggaagtcgg
      781 gtacgtgcca ggcatgaaga tggataatcc ggcccgaaac ttctcgctga tggctggcaa
      841 catactcacc cacagttgct tcgatgtcca attcttcgtt taactcacgc accagcgcct
      901 gctgctggct ttcatccagc tcgactttac ctccggcaaa ctcccataat cccgcctgat
      961 cgctctgggc ggggcgttgc gcgagtaaaa ttttgccatc acgttcaatg atggcggcaa
     1021 caacttcaat cattttcata aattacacac gggcagataa gcgaaaaatg ccataatatc
     1081 cgcactgttg ctcaatcaga aaggctttat atttaaggag tgcctgtgct agaccgccat
     1141 cttcatcccc ggattaaacc gttgttgcat cagtgcgtgc gggttcttga taaaccgggc
     1201 attacgccag atggtttaac attagtggga tttgccatcg gcgtgctggc tctgccgttt
     1261 ctggcgctgg gctggtatct ggcggcgcta gtcgtcattt tgttgaacag gctgcttgat
     1321 ggtctggacg gtgcgctggc gcggcgcaga gggcttaccg acgcgggcgg ctttcttgat
     1381 atttctctcg attttctctt ttacgcgctg gtgccgtttg gctttattct tgctgcaccg
     1441 gagcaaaatg cgctggcagg cggctggttg ttgtttgcgt ttatcggcac gggcagcagt
     1501 tttctcgcct ttgctgcact ggcagcaaaa catcagattg ataaccccgg ttatgcacat
     1561 aagtcgtttt actatctggg gggattaacc gaaggcacag aaacgatctt actgtttgtg
     1621 ctgggatgtt tattccctgc atggtttgcg tggtttgcgt ggatcttcgg tgcgttgtgc
     1681 tggatgacga cctttacgcg ggtgtggagt gggtatctga cgctgaagtc gctccagcgt
     1741 cagtaattgc aatgttattt gctactgccc gggccgcgtt caccggttgc aaccggattt
     1801 tttggatcgc tgctccattc gtaccagccg ccgtcataca cggaaacatt cttccaaccc
     1861 atggcgcgtg cgtacataaa ggtttcggac gcgcgccagc cggtgccgca gtagaatgaa
     1921 acttgctgat ctggtttgat attccatgct ttccacatag cggtaatatc atcggcgcta
     1981 cgcatggtgc catccgggtt atagaaatct tccatatgcg tcgagtcgct accagcgtgt
     2041 ccccaacgtg ctccggctat ttcacctttt ggtttaatat agctgtaacc gctggtcgta
     2101 ccgataaatt ctggccacga acgaatgctc accagcgatg catcctggcg atgcagcagt
     2161 ccacgggcct gttccatatc gagcatcaac tgcggttgtg ccgggatctt cacgccgaaa
     2221 tccggttccg ctttcacttt cggtggcgtt cctcgctcaa caggcagtcc cgcgtcggac
     2281 caggtttgcc agccgccatc cagcagacgc acatctttca cgcctgcgta gagcataatt
     2341 tgcgccacac gcgctccagc gtatacgtca cgcccgtaca gaatgaccgt ggtgtcatgg
     2401 cgaatgccgt gttttgccag catcgctttt agttgttcat cagaaacttt gttccacagc
     2461 ggttcgcttt ccacttcgtt ggtatcgatg tagtcagcgc caggaatatg gctgataagg
     2521 taaagcttag gagcgcccta ggccgcttca atgactttcc agtcaccgac aggtttcgcc
     2581 gtaacctctt taccttgttg caggtcatgc agccattgcg gataaaccag ctgctcaaag
     2641 tgcggcagtt tttgtagacg ggaaggttcg cttagcgcgt cactcagaat ggagatatgc
     2701 gtaaaacctg ctttttgcag tcgcgttttg acggcgtcaa catctttgcc attaccgtac
     2761 agcgccaccg gggcatcggc tttcaggtta tgttgcttga tccacgcgtt gagctgttcg
     2821 gtgctcattt tgtccagcca gctagcagag aggtttaagg cggcaggttc atgaccagaa
     2881 gggccattta aggtttgtgg ccagccgtta taaaacgcgc tggggcgagt atctatcgct
     2941 ttgccatttt gttgctgaag ttggtcaagt gtaagaggct tcgccagttc agcggcccac
     3001 gaagaagcgc aagccagccc taaagccatt gccagcgcgg tcatttgaga aacacgtttc
     3061 atcgaaaatc ccgacattgt agttttgagt gcgccattct gaactgacgc attgagaaaa
     3121 actttgcgtt atcgcagttt gttgtaattt tctgaccact gcgccatatc cagaacagaa
     3181 ctatcagcag gaacatcctg gagatcgtgc gttacctgaa cgacggggat cgccagggcg
     3241 cgaacttcgc tgaacaccca ctggcgaaaa ttatcgcgca gagccacatc aagacggctg
     3301 aatggctcat ccaggagtaa cgcttttggc tgggcgagaa gggcgcgtag tagagcaacg
     3361 cgcgctcgct gaccgccaga caaagtggca ggatcctgat ggaaagctcc gtcaaggcct
     3421 gaacgctcaa gtgcatcatt cacggcatta cgtcgggcat tccccttaag tgtcgccggt
     3481 agcgccagca gtaaattttg cccgacgctg aactggtcaa ataacagtgc atcctgaaaa
     3541 agaatgccaa tctgacgctg tgcggtgggt agcatgtcaa tccgttgctc attgagccat
     3601 agctcacctg tacaagaaaa ctgtccggcc agtgcaccaa tcatccatga aaacagagtg
     3661 gattttccac agccagacgg ccccattaac gtgacgatgt cacctttatc aaccgtaaag
     3721 ttaacgtttg tcagcaagcg gctttctggt aaacgtagcg aaacattttt cacgcagagc
     3781 attagcggat tccttgtctg acataaccta cccattttgc gactaacgcg gtcagggcaa
     3841 aaataataag cggtaacaac agttgccata acgcctgggc ggcgagaata ccgttgctgc
     3901 cgccgctgct taatgccacc gcctcagtgg tgagcgtcgg aaaacgcccc gcgcccagcc
     3961 acagcgtcgg catatactgc acaatactga ctgaaaatcc caccgcgaag gcaatcagca
     4021 cagggcgcaa catgagtggg catttcacgt aaaagaagat tttggcccgc gaccagccca
     4081 gtgtttgcgc aatcaaaatt aaccgtgaat caatgcgctg ccaggcgggt tgcaggataa
     4141 acagcatcca cggcattacc cacagcagat gcccccagac caccgccgtc cagcttccat
     4201 ccaggttcag ccatagcgcc agcgtgtact ggcccgccac cagtggcaga gcgggcaata
     4261 gaatgggcaa ccatagccac aactggcgac gctgtgcccc ccattccagc cacagcagta
     4321 acaggagcaa agcgatgaat gttgctacca gccccattgt cagactgttg atgagtgctt
     4381 cactattgat cgtcgactga tccgcgagga tcgccagcag aaccacacac agcacaccgg
     4441 ttaagggtaa aaaactcgcc agcgtagtgc ctggtaataa aggtgtggca ggcttgcgaa
     4501 tgccatctac gcggggaata gtgcgccgcc agctgcgcca aaacgtaggc ggcgagtaac
     4561 agcatcagca gcagactggc gagtgcgcct ttcgtttgtt gatccgcgtc gccctgagtt
     4621 aaccactgcc agctaattac tgccagcgtc ggtggattac ctggcccaag aataatcgcc
     4681 acatccacga ccgaaagcga ccaggcaaca atcgccagca tcgccattgc cagcggctgt
     4741 agcccagcga atccagcaca atgacctgct gcaacagccg tttttcgctc aatactgcca
     4801 ctaagatcca cagcagaaat gcgctttctt tcactgcaag ggtgaggccc agaccgatcc
     4861 aaatcggtcc attggcggag tgaaatacgg gaaatagtca tatagcagcc ctccgtcagc
     4921 aaagagcagc agggcgctgg tggcaaaagc cacatgggga atggcgagca gccacggcag
     4981 acgggcgcac atacgctgcc atttcggccc aggccatagc gccacaatca ccagcagggc
     5041 aatcaacaat gccccgaccg ccgcgatggt tgtcgacacc agcgttgcca gtaatgcctg
     5101 cggtaactgt ggatcggcaa ataacgcctg ccagcgtgtc aacgacaacg caggcgatat
     5161 cagcgtgaga gctgccggga tcagcggtgc ataaatcacc gccaccatcg cccacagcaa
     5221 aaagattaat gcgtaccgta acggcgtaac cattcttgtt ccagtgcatt cacccatcct
     5281 gcgtgcggtt cagccagtac cggcggcaga tcctgcggca ttcttgattg caatatttcg
     5341 cgctgcccgt caggcagttt ttgcggatcg agaacagaag gatcgcccca gacaacggga
     5401 tctgctttac gcagttgcgc atcgggtgag agcaggaaat tggcaactac cttcgccgca
     5461 gcactggcat tcgcgttggc tggaatggtg acgaaatgca cgttgccaat catcccctcg
     5521 cgaaagccaa aactgtaact gcttgccggc aaatcgccgc tggcgatttt ttgctgcgca
     5581 tgtgcggggt taaaggtcag cgacaggcgc aatgtgccgg ctttcagcag agcatccatc
     5641 cgcgcgggcg aaggcgggaa atcctttcct tcgcgccaca aatacggatg cagtgcatca
     5701 agatattgct acaagggcgc agtgacacgg gcgaaagtcg catcgtccgg cgcttctttt
     5761 aatgcggcgg gatctggcgt cagcataatc agcaactgtt caagaaacgc cgtgccggta
     5821 aagtccggtg ggcgcggata agtaaccgtg ccgggattgg ctttagcaaa ctccagtaag
     5881 gcttgcggcg tttgtggtgg ctgtggcgta acatcgcggc gggcgataaa cgtcagttgt
     5941 gcgccgcccc agggcgattc cgcaccttgt gtcggcactg aaaaatctcc ccgcaccggc
     6001 agctgtgtgt cgacatagcg ccagttgggc agagtctccg cccagcccgt ttgtaataaa
     6061 ttggcctctt tta
//
LOCUS       DAJTMX010000189         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTMX010000189 DAJTMX010000000
VERSION     DAJTMX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.189"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            430..1338
                     /locus_tag="OO145_003888"
     CDS             430..1338
                     /locus_tag="OO145_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5468824.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO145_003889"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO145_003889"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5468825.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO145_003890"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO145_003890"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5468826.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO145_003891"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO145_003891"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5468827.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OO145_003892"
     CDS             complement(5673..>6052)
                     /locus_tag="OO145_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468828.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJTMX010000190         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTMX010000190 DAJTMX010000000
VERSION     DAJTMX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.190"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..393)
                     /locus_tag="OO145_003893"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO145_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO145_003894"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO145_003894"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5468829.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCINKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO145_003895"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO145_003895"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5468830.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO145_003896"
     CDS             5797..>5983
                     /locus_tag="OO145_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5468831.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1354 c         1018 g         1771 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatt gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTMX010000191         5967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTMX010000191 DAJTMX010000000
VERSION     DAJTMX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.191"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            348..641
                     /locus_tag="OO145_003897"
     CDS             348..641
                     /locus_tag="OO145_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5468832.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO145_003898"
     CDS             713..1372
                     /locus_tag="OO145_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5468833.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO145_003899"
     CDS             1464..1910
                     /locus_tag="OO145_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5468834.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO145_003900"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO145_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO145_003901"
     CDS             3390..3809
                     /locus_tag="OO145_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5468835.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OO145_003902"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OO145_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5468836.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO145_003903"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO145_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5468837.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..>5967)
                     /gene="nhaB"
                     /locus_tag="OO145_003904"
     CDS             complement(5799..>5967)
                     /gene="nhaB"
                     /locus_tag="OO145_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="involved in regulation of intracellular pH under
                     alkaline conditions; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton antiporter"
                     /protein_id="HCR5468838.1"
                     /translation="LTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTE
                     WFMQMGWIATL"
BASE COUNT         1589 a         1336 c         1472 g         1570 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagc
//
LOCUS       DAJTMX010000192         5955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTMX010000192 DAJTMX010000000
VERSION     DAJTMX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.192"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            223..882
                     /locus_tag="OO145_003905"
     CDS             223..882
                     /locus_tag="OO145_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5468839.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="OO145_003906"
     CDS             complement(1083..1460)
                     /locus_tag="OO145_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5468840.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..4493)
                     /locus_tag="OO145_003907"
     CDS             complement(1527..4493)
                     /locus_tag="OO145_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5468841.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4496..5056)
                     /locus_tag="OO145_003908"
     CDS             complement(4496..5056)
                     /locus_tag="OO145_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5468842.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5182..5532)
                     /locus_tag="OO145_003909"
     CDS             complement(5182..5532)
                     /locus_tag="OO145_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5468843.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5735..>5955)
                     /locus_tag="OO145_003910"
     CDS             complement(5735..>5955)
                     /locus_tag="OO145_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5468844.1"
                     /translation="EQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTT
                     MIYTHVLKVGGAGVRSPLDALPPLTSER"
BASE COUNT         1195 a         1647 c         1793 g         1320 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc
     2101 ggcttgagcg cgtcataggc ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggattc gtggtacagc
     2281 aggccgtcga gcacgtaggt tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat
     2341 ggcgcgtatt ggtcggagat gtgggtgtag aaagtccgtc ctgggctgct gccatatttt
     2401 gggttgatgt gccccgtgct ctttgccttg ctagcggttc ggaaattctg tccgtccgat
     2461 gatgatgtgg tgccatcgcc ccagtgcccg gcaaagggat gccgaaactg agcgttgacc
     2521 agttcagcca acgctgtcga gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg
     2581 agcttcgcgt aggtcgtgcc ggggcaggac tcggccatct tggtcaggcc caggttgatc
     2641 gcgtcggcca ggatcgtggt caacaacagg ttcttgtcct tggccagatc gcccgatttc
     2701 aagtgcgtga agtgccgggt gaagcccgtc cactcatcga cttcgagcag cagttcggtg
     2761 atcttgacgt gcggcaggac catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc
     2821 accgccgcat ccagcggcgt gatcttcaag cccgactcgg tgatgatggc atccggcagg
     2881 tcgttggctg ccgccatgcg gttgacggtg gcaagttgtg cttccagcag cgtcagccgc
     2941 tcatgcagat attgttcgca gtcggtggcc acggccagcg gcaattcgct ggactgcttg
     3001 aggctggtga acttctcggg cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa
     3061 ccctgcaccc agatgtcgcc cgagcgcagg gagttcttca actcggacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg
     3181 atgaagccgg tcggtgcatc ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc
     3241 agcacctcaa tggcatcaag cacgtttttg gcggcgggcg cggcccgcag cttgagcacg
     3301 gcaaggaatt ccggtgcata gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg
     3361 aaatcgaagt catcgggttg cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa
     3421 tcccaggaca tgacggcctc gatggcggca aacgcatcgc ggcctgattg cttggcgtcg
     3481 atcagcgcct gaccgatgcg cccgtacaga cgtaccttgg cgttgatggc cttgcctgac
     3541 gcctggaact gctgctgatg cttattcttg gcagcgttaa acagcttacc caggatgcgg
     3601 tcgtgcaggt cgatgatttc gtcggtgacg gtggccatgc cctcggtggc cagcgccacg
     3661 agagtggcgt agcgccgttg cggctcgaat ttggccaggt cggcgggtgt catctggccg
     3721 ccctcgcggg caatcttgag caggcggttc tggtgaacca gccgctcgat gccggtaggc
     3781 agatcgagtg cctgccatgc cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt
     3841 ggcttggccg gagactggcg caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc
     3901 ttgagcagat cgtcgaggcg gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg
     3961 tagatgcgcc ggttagcacg ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg
     4021 gcgggcagaa tgaccgactg ccgccgcagg tgcccgatca aggcgctggc cagcacgatg
     4081 cctttgtcgg tttgcatcgc cagctcggtc agcatctgga cggcctgccg gtaatggctc
     4141 atggtgaagg gccggaaacc gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc
     4201 tgctcccgct ggccgtactc gttccagctt tcgacgccga ccttgagctg gtcggcgacc
     4261 agcttcaaca agggcgggaa cggtagttca tcgacgccca ggatgacgcc gggaaagcgc
     4321 aggtaacaga gctgcaccgc gaagcccagc cgattggctg gcccgcgccg ctgtcggatg
     4381 atcgagaggt cggtatcgtt gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc
     4441 aacgccagca ggctttcccg ctcggcggcg gacaggatgg aacgacgtgg catatttact
     4501 gatccgttct caagtattga tacagggttt cgcgactgat tccgaattca cgagcaagct
     4561 tggtcttttg ctcgccagcc tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca
     4621 gggatttctt cctgccacgg taagccccgc gttgcttggc gagcgcaata ccctcgcgct
     4681 gacgctcgcg gatcagggcg cgctcgaact cggcgaacgc gcccatcacc gagagcatca
     4741 ggttcgccat cggagagtct tcgccagtaa aactgaggtg ttccttgacg aattcgatat
     4801 gcacgccgcg ttgtgtcagc gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca
     4861 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atcagcgctt
     4921 ccagttgcgg acgcttgaca tccttgccgg atgccttgtc gctaaaagcg cgatcaacct
     4981 tgacgccttc cagttgccgt tccgggttct ggtcgaaggt gctgaccctg atatacccaa
     5041 tgcgctgtcc agtcatggaa ttccctgcaa aatgtcaggg aagactctat gaccttcaac
     5101 gagatatgtc aataaattca aaattcaatc ctatcctgac gcaatttaca catggcatct
     5161 gacatcaggt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac
     5221 gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact ccagcccgca
     5281 gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt
     5341 gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt tcctccattg
     5401 gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga aaccctgcac
     5461 cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc
     5521 taccacctcc attgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
     5581 tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg ccatcagcgg
     5641 acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg
     5701 ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg aggggcggca
     5761 gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc gtgtaaatca
     5821 tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc
     5881 tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga
     5941 tgcctgcttg ttcta
//
LOCUS       DAJTMX010000193         5834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTMX010000193 DAJTMX010000000
VERSION     DAJTMX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.193"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(36..1391)
                     /locus_tag="OO145_003911"
     CDS             complement(36..1391)
                     /locus_tag="OO145_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5468845.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(1395..1679)
                     /gene="gatB"
                     /locus_tag="OO145_003912"
     CDS             complement(1395..1679)
                     /gene="gatB"
                     /locus_tag="OO145_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5468846.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(1710..2162)
                     /gene="gatA"
                     /locus_tag="OO145_003913"
     CDS             complement(1710..2162)
                     /gene="gatA"
                     /locus_tag="OO145_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5468847.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(2172..3434)
                     /gene="gatZ"
                     /locus_tag="OO145_003914"
     CDS             complement(2172..3434)
                     /gene="gatZ"
                     /locus_tag="OO145_003914"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5468848.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            complement(3463..4317)
                     /gene="gatY"
                     /locus_tag="OO145_003915"
     CDS             complement(3463..4317)
                     /gene="gatY"
                     /locus_tag="OO145_003915"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5468849.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(4625..5677)
                     /gene="fbaB"
                     /locus_tag="OO145_003916"
     CDS             complement(4625..5677)
                     /gene="fbaB"
                     /locus_tag="OO145_003916"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5468850.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            5717..>5834
                     /locus_tag="OO145_003917"
     CDS             5717..>5834
                     /locus_tag="OO145_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5468851.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWID"
BASE COUNT         1526 a         1528 c         1348 g         1432 t
ORIGIN      
        1 ttgttaaggg ggataacctc cccctcaggt aaaaattatt ctgcgagaac ggctttctct
       61 tgtttagtaa aaccacgcgc tctacgccag gtcatgaaaa taccggtcag ataaattgcg
      121 ccgataataa tgaaaccggg aatattttgc ggggagaaaa cctgaatcag taaccaggta
      181 atgggagaac cgccctgatc cattgaagcc accatacccc cggcttttaa cgccccagca
      241 ttagccgccg gttgggtgtg caaaccaata gtttgcgtcg cgatccacag ggtgatgctc
      301 ataatgatga cacccgagat taaggtgcgg aacagatttc cacgatgcac cgcgaccgcc
      361 atcgccacga agaagccgat agtggcaaga tcgccaaacg gcagtacctg attacccgga
      421 acacaaacag caattaaaat ggtgagcgga ataaaaatca ggcttgccga taccaccgcc
      481 gtatgcccca gcagtaatgc gggatccagg ccaatcagga actcctgacc gccgaacttc
      541 gcctgtaaac gactacgggc ctgcttagcg atgggcgtta aaccatccat gatgggttta
      601 atcatccgtg gcatcagcag cataactgcc gccgttttta ccgccagctg caatacacct
      661 ttgacatcgt aacccgcgag gatgccgata atcagcccca tcacaaaacc cacggtgaca
      721 ggttcgccaa aagggccaaa tttgcgctga atatcgtcgg cgctaaattt aattcggtta
      781 acgcctggga ttttctcgat gatagcatcg accagcaccg caatcggccc catatacgcc
      841 gacgtaccgt gcggaatagc aataccttcc agctcaaaga aatttcgggt atcgcgggca
      901 aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta ccacacccgc catccctatc
      961 atccatgaac cggttgccag gtgcagcaac gcgccggtga aggtcatatg ccagatattc
     1021 cagatatcaa catttaccac ccgcgtcata cgggtcagta acatcgccac gttaaccaat
     1081 atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc aggtcattgg tgaagagccc
     1141 ggccagccga catcgaccac atgcagattc aggtcgaaat tttccgccat cgctttcgcc
     1201 gccggaccaa tggaatccag cattaagcca atcacaaggc caatgccaac aaagccaatc
     1261 ccgatatgca gacccgcttt aaagcaatcg cctgccttca tgcctaatat tttagaaaaa
     1321 ataatgatga caaccggcag catcactgta gggccgaggt cgagaatata acgcatgact
     1381 tctgaaaaca taggtcatcc ctgtaagata gtcagaattt tattttgtaa tgcttcgata
     1441 ccgacaccag aaacaaaagg catgccgtga actaacggaa tatcgccaaa actacgatcc
     1501 accctggcag tggtacatat caaatgcaca ccatccatat aggtttctat ttcattaacc
     1561 cgacactgga ttaattcaac aggaatatta tgactctgac acaactcttt aatttcttcc
     1621 gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga caataatctt gcgtttcata
     1681 aatagtcctt tttattgtta cagagggatt tattcgtctg aatctaaaac atactttgtg
     1741 aagtattcct ttaactgagt ttcaggaaga gtaattagtg tctcgacgat atcgggctgt
     1801 tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct gctgcggatt ttccacaatc
     1861 aacgcaataa ccaacgatac cgccacgtcg ttatcatcat ccgcttgctg aaaatgaact
     1921 ttatttgttg gccttaacag ataaatggct gacgacttag catgaatcgc ctcacaatgc
     1981 ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag ggaattctgc ttctctggca
     2041 attaatgcct gtggccaggt atcatgaacc acacctttcg cgagcatctc attaccgata
     2101 taggttaaaa cttcgctacg atcgacaaaa gaaattccgc tacgaacaaa caggttagtc
     2161 atataccgtc cttattccgc acagccgtag cgataggcgc gcaaaacatc ataaatttta
     2221 ttcataataa gctgatgcgg cattgctgat aattccccgg actgaatgcg ttcaaattgt
     2281 ttgggaagat actgactaat catgcccagt gggatgtcca cgccttgcag attcaccatc
     2341 atcgtttcaa cgctattttt aatccgacta tgcggccagt aataacgaat acgatccgac
     2401 aggctgtaac gaatatccag taatgaatcg ttaaaacccg tgcgataata ttttttccag
     2461 tattgcggtt cgtcgagcat cacttcttca attaccgcca ggcaaccgct gcgattttcg
     2521 ggggcgataa gttcctgctc aatttgtgcc agtgcaaata tcgcctcgcg taaagcaaag
     2581 gttaatgcgg gaccgacttt caatattgca aagtgatcgc ggactaattc ccaataagcc
     2641 gtccgggtct ggtaatcggt agaatgtgct tcataaacca ttcgggtgtt ttctatccat
     2701 tgcgccagcg cctgcgcttc ctgcggctga taatggataa tattgctgtg atcaaattcc
     2761 acacccggct gcaccacgat ggcaatcaca cgtgttaacg cctctgtcag cccacgggca
     2821 ataaaggcct tttgatgcgt acgtaaagta ttggcggcat cttcaacatg ggtgatgtgt
     2881 actgactgaa tggcgctggc ctcaccgccc ggaaccggaa cttcagtgcc aatgacatag
     2941 ctcagttgct cacgctggca atctgtcgcc acactttccg cagcaaagca aagcacagca
     3001 gctcgttccg caaccgtttc tggtgctaac ggtatggtat cccccgcgca ggacattgac
     3061 gcatcaagat gaattttact gaagccagca cgaacatatg cttttaccag ctcgacggat
     3121 ttttccatcg ccgcatccgc attttcttgc tgccagcagt ttggtcccag atgatcgccg
     3181 ccgagaataa tgcgttcgcg tgcaaaccca actttatcgg caatcgtaaa aacaaattcg
     3241 cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt gattgacctg gtttgacgtt
     3301 gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg ccagcgccgc ttcgataacc
     3361 aacggatggg cagaacagac tgaacatatg ccgatatgtt caccagcttt atgccgggca
     3421 attaacgttt tcatgttttt tccttgttaa atggcaggtg cgttatgccc tgccctcgca
     3481 gccacaatcg gcaatcactt tgctcaccac atcgcgcatt gcggatttag ccgactgcaa
     3541 ataatcccgg ggatcggtcg cttcggggtg ctcggtcagg taatttttta acgactgcaa
     3601 gaaggcattt ttcagctccg ttgcaacgtt gattttgcat atccccagtt tgatggtttg
     3661 ctgaatatcc ttagtcgata atcctgacgc gccatgcagc accagcggta agttcaccca
     3721 ctggcgaatg ttctccagtc gagaaaaatc aagcaccggt gcgctggcat acataccgtg
     3781 agccgtgccg atggcgaccg ccagggaatc aatcccggtt gcctcggcaa attcacgcgc
     3841 ctgtgcgggg ttggtgtaca acgcatcgac ttcattgact tgcacatcat cttcctggcc
     3901 gccaagttgc cccagctccg cctcgacgct gacatcaaag cgatggcaaa aatccaccac
     3961 ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc gaggcgtcaa tcatgactga
     4021 gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat ttcgtgtgat ggtcgagatg
     4081 aattgccagt ggatggtgat attgcttcgc catcgcgctg accagcgcca acagattttc
     4141 tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg atgaccggcg catgcaggtt
     4201 ggcagcggtt tccaccacca cttgcatcgt ttcgagattg tgaatattga atgccggaac
     4261 cgcataaccg ccgcgctgtg cgttgttcag catctgcttt gtcgatacca cgtacatttt
     4321 catatcttgt cgtttgcttt tgatttcaaa atataatgaa attatttgct tttaaatgtc
     4381 gagataatga tcgcaaaaac aacaatatga aaattgttcg aggagtgaaa ggcaaaaaaa
     4441 cggcctcccg atagggaagc cgtagcaaag tgcgcgtgtt tttatgccgg atgcggtgta
     4501 aacatacgtg gcagtgctat gtaggcatga taagacgctt ccgcgtcgca tcaggcatag
     4561 gttgccggat gcagtgtaaa cgtcttatcc gtcctacggg gtggtgctgt tcgtatgagg
     4621 cgtatcaggc aatagtaatt ttgctatcga gataaacgtc ctgcacggca ttaatcagtt
     4681 tcacgccgtc agccatcgat ttcttgaacg ccttacgtcc aagaatcagc cccattccgc
     4741 ctgcgcgttt gttgataacc gcagtacgca ctgcatcgct gaggtcagtt tcaccgcctg
     4801 cagcaccgcc ggagtttatc aacccagccc ggcccatata gcagttagct aactgataac
     4861 gcaccagatc aatcgggttt tcgctggtta acttgctgta cacgcgatcg tcggtataac
     4921 cgtaattaat cgctttatag ccgccgttat tttccgccat tttttgtttg acgatatctg
     4981 cacctatggt tgccgccaga tggttcgcct gaccagtcag gtcggcggaa acatggtaat
     5041 caacgccatc tttcttaaag gcggagtttc gcaaataggc ccacagcact gtcaccatgc
     5101 ccagctcgtg cgcgcgttca aaggccgcag aaatttcttc aatctggcga cgtgactctt
     5161 ccgagccaaa atagatagtc gcaccaaccg ccaccgcgcc catgttgaag gcctgctcca
     5221 cgctggcata cagcgtttga tcgtaggtat tcgggtaact tagcgtctcg ttgtgattaa
     5281 gtttgacgag aaatggaatg cgatgcgcat agcgccgcga taccgacacc aacacgccgt
     5341 aagttgatgc cacacagtta cagcccgctt cgatcgccag ttcaacaatg tttttcgggt
     5401 caaagtagag cgggttagca gcaaatgaag ctccggcaga gtgctcaacg ccctggtcaa
     5461 ccggcagaat agaaagatat cctgtgccag ccagacgccc ggtgttgtac aacgtctgca
     5521 tattacgtaa caccgctggc gggcgattat tgtcaatcat cacgcggtct acgtagtcat
     5581 gtccggggag ataaagctgg tcagaaggaa tagtcataca acggtgctgt aaaaggttgt
     5641 cggcgtcttt gccaagcaac tgcgcaatat ctgtcattac tatgctcccg taaattccga
     5701 ttcaagggga agttaaatga aaacagaacg tattctcggt gctctttatg ggcaggcgtt
     5761 aggggatgcg atggggatgc cctccgagct ttggccacgc agccgcgtta aagcacactt
     5821 tggctggatt gacc
//
LOCUS       DAJTMX010000194         5788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTMX010000194 DAJTMX010000000
VERSION     DAJTMX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.194"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..244)
                     /locus_tag="OO145_003918"
     CDS             complement(<1..244)
                     /locus_tag="OO145_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468852.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEL"
     gene            complement(241..459)
                     /gene="ybdZ"
                     /locus_tag="OO145_003919"
     CDS             complement(241..459)
                     /gene="ybdZ"
                     /locus_tag="OO145_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5468853.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(462..1664)
                     /gene="fes"
                     /locus_tag="OO145_003920"
     CDS             complement(462..1664)
                     /gene="fes"
                     /locus_tag="OO145_003920"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5468854.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            1907..4147
                     /gene="fepA"
                     /locus_tag="OO145_003921"
     CDS             1907..4147
                     /gene="fepA"
                     /locus_tag="OO145_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5468855.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4322..4942
                     /gene="entD"
                     /locus_tag="OO145_003922"
     CDS             4322..4942
                     /gene="entD"
                     /locus_tag="OO145_003922"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5468856.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(5064..5216)
                     /gene="hokE"
                     /locus_tag="OO145_003923"
     CDS             complement(5064..5216)
                     /gene="hokE"
                     /locus_tag="OO145_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5468857.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(5567..5719)
                     /gene="hokE"
                     /locus_tag="OO145_003924"
     CDS             complement(5567..5719)
                     /gene="hokE"
                     /locus_tag="OO145_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5468858.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
BASE COUNT         1464 a         1533 c         1501 g         1290 t
ORIGIN      
        1 gcagttcgaa tatcagcgca tcatcgaccc attgccagac ttcgccgtta tcttccgtaa
       61 aacgcatccg caaagtatct gcttgcgcta atccggcaac caccgcgcgg gccagtaatg
      121 gcgcatcaac ctctccggtt aactcaacgt aatgcgccac gctccaggcg gagggtaaat
      181 ccgacagttt ttctgccatc cagatgccgg gctgtgcggc gaccaaaggt aaatgctggc
      241 tcattgtgcc tcctgcaact gggtgaaatt cgccggtgtc agagtacgcc agtgggcttc
      301 aagccactgc tggcaggact cttgtgactg cggctgacac acaatgtccc agcctgccgg
      361 taaggcgcat tgttgcggcc acagactgaa ttgcccctgc gcattgcgca aaatgtaaaa
      421 cgctccctgt ggatcatcga agggattact gaatgccata ttcaactcct gtcatggaaa
      481 agtggttgcc agaggtcgat taacccctgc atcaagccac cgcgccaaca aagcgcatca
      541 tgtccgccgt caacctgacg ccagaaaatg gattctttta tagggtgtaa ttgcgcatac
      601 agcgcctgat tggcccgcat gatcatcggc tcgcgaatac ccgcttccag cacaatgcgc
      661 agaccttcgg cgctaacttc accagctttt agcttttcaa gtaacacgcc ctcttgctgc
      721 ccgccccgat gcggccacca gtacgatcct gactggctta atacacagcc aaagcgttca
      781 ggccagtgca gtccggcata cagcgcggac agcccaccaa aactctgccc ggcgaccacg
      841 gtgcgatcgg cgcgatcgct aaaaggggca ataactttca ccaggggtaa taactcttgc
      901 tgtactgcca gccagaaatc cgcattacac ggcagttcgt gggcgcggtg cgtggtgtcg
      961 atagcgtcga tcaacacata cacggcggga ggaagttgct gacgatgggt cagcgaagtc
     1021 agcacaggcc agacgggcat actttgcgcc caaaattcgc catcgagcaa aactgccagc
     1081 gggcgttctt ctgctgttac atcgccggtg gtaaaaatcc atacacgacg tgaatttttc
     1141 aaccgttcac ttttccagat aatttctttg gctggtattt ctggcgcttg cggacaatcc
     1201 catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag aaacagcgtg ccctagcccg
     1261 cctttccagc tttgtggatt cagcggatcg gctattgcct ggggtaatag ttttcgccag
     1321 ccttcacgta attcgaggcg atcgggggat ggtgcagaaa aaatgtcatc gcgttcggtg
     1381 ggaataaagc agtagctgcc gcgccagttg gcattgagtt gtgtcgtcca ctgccagaca
     1441 tcagtacctg caattcgctg catcgactgg ggctggctgt tctgatgatg atcggtcaca
     1501 ccagtgatgt agacccatac gcgctttatc gtcgagtatt cttcagaacc ttggggatca
     1561 cgccaccaga aagtgacctc aaacatttcg tcattcagac gctgccattc cgggccatgt
     1621 ttcgactgcc accagctctc acttcctact tttaacgccg tcacaccata accccatgtt
     1681 tactgtgcaa tttttcattg attgcagaaa tatattgata atgttattaa taactatttg
     1741 catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga cattaattaa ccaactgcac
     1801 tgcgtgtctt tcaggatcaa aggttttcgc ggtaacggga tgcgtcgtgt tgatgacgac
     1861 catgcccgac agttgcaatt cgtggcaaaa atgcaggaat aaaacaatga acaagaagat
     1921 tcattccctg gccttgttgg tcaatctggg gatttatggg gtagcgcagg cacaagagcc
     1981 gaccgataca cctgtttcac atgacgatac cattgtcgtt accgccgccg agcagaactt
     2041 gcaggctcct ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc cggttgcccg
     2101 cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac ctgaccggta actccaccag
     2161 tggtcagcgt gggaataacc gacagattga tattcgcggt atgggtccgg aaaacacgct
     2221 gattttgatt gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg gctggcgtgg
     2281 cgagcgcgat acccgtggtg atacctcctg ggtgccacct gaaatgattg aacgtattga
     2341 agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg gcgtggttaa
     2401 catcattacc aaaaaaggca gcggcgagtg gcacggctcc tgggacgcat atttcaatgc
     2461 gccagaacat aaagaggaag gtgccaccaa acgcactaac tttagcctga ccggtccgct
     2521 gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac aaaactcagg ctgacgcgtg
     2581 ggatatcaac cagggccatc agtccgcgcg tgccggaacg tatgccacga cgttaccagc
     2641 cgggcgcgaa ggggtgatca ataaagatat taacggcgtg gtgcgctggg acttcgcacc
     2701 attgcaatcg ctggaactgg aagcgggtta cagccgccag ggtaacctgt atgcgggcga
     2761 tacccagaac accaactctg acgcttacac tcgctcgaaa tatggcgatg aaaccaaccg
     2821 cctgtatcgc cagaactacg cgctgacctg gaacggtggc tgggataacg gcgtgaccac
     2881 cagcaactgg gtgcagtatg aacacacccg taactcgcgt attccggaag gtctggcggg
     2941 cggtaccgaa gggaaattta acgaaaaagc gacacaggat ttcgtcgata tcgatctcga
     3001 tgacgtgatg ctgcacagcg aagttaacct gccgattgat ttcctcgtta accagacgct
     3061 gacgctgggt acggagtgga atcagcaacg gatgaaggac ttaagttcca acacccaagc
     3121 actgaccgga acgaataccg gtggcgctat tgatggcgtg agtgccaccg accgtagccc
     3181 gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac aacatggagc tgactgacag
     3241 caccatcgta acgccggggc tgcgtttcga tcatcacagt attgtcggca ataactggag
     3301 cccggcgctg aacatatcgc aaggtttagg cgatgacttc acgctgaaaa taggcattgc
     3361 ccgcgcctat aaagcgccga gcctatacca gactaacccg aactacattc tctacagtaa
     3421 aggtcagggc tgctatgcca gcgcgggcgg ctgctatctg caaggtaacg atgacctgaa
     3481 agtagaaacc agcatcaaca aagagattgg tctggagttc aaacgcgacg gctggctggc
     3541 gggcgtcacc tggttccgta acgattatcg caataagatt gaagcgggct atgtggctgt
     3601 agggcaaaac gcagtcggca ctgatctcta tcagtgggat aacgtgccga aagcagtggt
     3661 tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa acggtgatgt ggaccaataa
     3721 catcacttat atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt cgatcatccc
     3781 ggagtacacg ttgaactcaa cgctgagctg gcaggcacgg gaagatttgt cgatgcaaac
     3841 gaccttcacc tggtacggca agcagcagcc gaagaagtac aactataaag gtcagccagc
     3901 ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt gtgggtctga gcgcgacctg
     3961 ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac aacctgttcg acaaacgttt
     4021 gtggcgtgca ggtaatgccc agaccacggg cgatctggca ggagccaact atatcgccgg
     4081 tgccggcgcg tatacctata acgagccggg acgtacgtgg tatatgagca ttaatactca
     4141 cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc gttcacgccg gatgcggcgt
     4201 gaacgcctta tccggcctac aaagatcttg ccaattcaat atattgcagg atcacgtagg
     4261 cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtctcga atatggtcga
     4321 tatgaaaact acgcatacct ccctcccctt tgccggacat acgctgcatt ttgttgagtt
     4381 cgatccggcg aatttttgtg agcaggattt actctggctg ccgcactacg cacaactgca
     4441 acacgctgga cgtaaacgta aaacagagca tttagccgga cggatcgctg ctgtttatgc
     4501 cttgcgggaa tatggctata aatgtgtgcc cgcaatcggc gagctacgcc aaccggtgtg
     4561 gcctgcggag gtatacggca gtattagcca ctgcggggct acggcattgg ccgtggtttc
     4621 ccgtcaaccg attggcgttg atatcgaaga aattttttct gcgcaaacag cgaccgaatt
     4681 gacagacaac attattacac cagcagaaca cgagcgactc gcagactgcg gtttagcctt
     4741 ttctctggcg ctgacacttg cattttccgc caaagagagc gcatttaagg caagtgagat
     4801 ccaaactgat gcaggttttc tggactatca gataattagc tggaataaac agcaggtcat
     4861 cattcatcgt gagaatgaga tgtttgctgt gcactggcag ataaaagaaa aaatagtcat
     4921 aactctgtgc caacacgatt aattgacaac atctggtacg attcgcccgc agccatcact
     4981 gactgcgggc gaaagtgtaa agcaggtgcc ttaccactct gacctgacaa ccggatatgc
     5041 ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcaagaac agccttaaac
     5101 tcaatattac gttcctttac ggtgaactca cacagcgagt ctccgaccag aagcgtaaat
     5161 cccagcaccg tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt
     5221 atattgcctc catgcgaaga agggactacc atccgagttg ttgaggttcg gagtggcagc
     5281 cccaggattg atagaaatat ctcttggggc ttcatctttc caaacctcag agttaacctg
     5341 aaaccggaaa gcttcaggca cccgccgtta tcttaccctt ccttagcaaa atagctgttt
     5401 atttatacag tgagtcctgt ttcggacttc tgtcggcaaa actcatcatt gctcttaaaa
     5461 cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt gccttaccat
     5521 cctgacctga caaccggata tgcggggatt actccccgca caacggctac ttcttcggtt
     5581 cgtaagcgag aacagccctg aactcaatat tacgttcctt caccgtaaac tcacacagcg
     5641 agtctccggc cagaagcgta aatcccagta ccgtcaaaca cagcactatg actgccacaa
     5701 gggcatattt cgtcagcatg tttatattac ctccatgcga agaagggact accatccaaa
     5761 ttgtctggtt tggagtggca gccccagg
//
LOCUS       DAJTMX010000195         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTMX010000195 DAJTMX010000000
VERSION     DAJTMX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.195"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..241
                     /locus_tag="OO145_003925"
     CDS             <1..241
                     /locus_tag="OO145_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468859.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO145_003926"
     CDS             complement(238..1179)
                     /locus_tag="OO145_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5468860.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO145_003927"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO145_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO145_003928"
     CDS             complement(1684..2904)
                     /locus_tag="OO145_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5468861.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO145_003929"
     CDS             complement(2908..3651)
                     /locus_tag="OO145_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5468862.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO145_003930"
     CDS             complement(3542..5008)
                     /locus_tag="OO145_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5468863.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO145_003931"
     CDS             complement(5008..5241)
                     /locus_tag="OO145_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5468864.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO145_003932"
     CDS             complement(5229..5708)
                     /locus_tag="OO145_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468865.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTMX010000196         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTMX010000196 DAJTMX010000000
VERSION     DAJTMX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.196"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..170)
                     /locus_tag="OO145_003933"
     CDS             complement(<1..170)
                     /locus_tag="OO145_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468866.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO145_003934"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO145_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5468867.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO145_003935"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO145_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO145_003936"
     CDS             complement(3616..4383)
                     /locus_tag="OO145_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5468868.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO145_003937"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OO145_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="OO145_003938"
     CDS             5484..>5702
                     /locus_tag="OO145_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5468869.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAJTMX010000197         5599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTMX010000197 DAJTMX010000000
VERSION     DAJTMX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.197"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..410
                     /locus_tag="OO145_003939"
     CDS             <1..410
                     /locus_tag="OO145_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5468870.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OO145_003940"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OO145_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO145_003941"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO145_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5468871.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1331 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DAJTMX010000198         5582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTMX010000198 DAJTMX010000000
VERSION     DAJTMX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.198"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            29..244
                     /locus_tag="OO145_003942"
     CDS             29..244
                     /locus_tag="OO145_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5468872.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="OO145_003943"
     CDS             237..623
                     /locus_tag="OO145_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5468873.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="OO145_003944"
     CDS             631..1317
                     /locus_tag="OO145_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5468874.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OO145_003945"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OO145_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5468875.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="OO145_003946"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="OO145_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5468876.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="OO145_003947"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="OO145_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5468877.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4044..4415
                     /locus_tag="OO145_003948"
     CDS             4044..4415
                     /locus_tag="OO145_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5468878.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4425..>5582)
                     /locus_tag="OO145_003949"
     CDS             complement(4425..>5582)
                     /locus_tag="OO145_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5468879.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1543 a         1030 c         1215 g         1794 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aa
//
LOCUS       DAJTMX010000199         5571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTMX010000199 DAJTMX010000000
VERSION     DAJTMX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.199"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            525..1703
                     /gene="ltrA"
                     /locus_tag="OO145_003950"
     CDS             525..1703
                     /gene="ltrA"
                     /locus_tag="OO145_003950"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5468880.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1786..1965
                     /locus_tag="OO145_003951"
                     /pseudo
     CDS             1786..1965
                     /locus_tag="OO145_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1995..2477)
                     /locus_tag="OO145_003952"
                     /pseudo
     CDS             complement(1995..2477)
                     /locus_tag="OO145_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2733..3713)
                     /locus_tag="OO145_003953"
     CDS             complement(2733..3713)
                     /locus_tag="OO145_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5468881.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3713..4912)
                     /locus_tag="OO145_003954"
     CDS             complement(3713..4912)
                     /locus_tag="OO145_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5468882.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5234..>5571)
                     /locus_tag="OO145_003955"
     CDS             complement(5234..>5571)
                     /locus_tag="OO145_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5468883.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1539 a         1270 c         1316 g         1446 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtagaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt gggttcggga agctgaacga aagtaaactg
      181 tccgatgaag catcgaaatc ttagatgatg ccaaaatggg gccgtacagc cgtcccctga
      241 taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg cagcaggatc
      301 ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc gggaaacagg
      361 ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg aagggatggg
      421 gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga tgcaacgaaa
      481 atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc gtcgcaacaa
      541 gggagccccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa cgtgatggca
      601 atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca ccagtgctgg
      661 aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca acagtatccg
      721 atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac ccgattttcc
      781 atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg aaacaatgtg
      841 ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg tttttcgacc
      901 atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct aaatgggtca
      961 tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat ggagaattaa
     1021 taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt gctaacttgt
     1081 tccttcacta tgcatttgat ttgtggatgg aaagagaata tcggggggta ccgtttgaga
     1141 ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca aggctaaaga
     1201 acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg aaaacgaata
     1261 tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc tttctcggat
     1321 atgacttcaa agtgcgtacg ctgaagaatt tcaacggcga acggtaccga aaatgcatgc
     1381 cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag tggcgtatac
     1441 atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg atagtgagag
     1501 gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga ctgtggagtg
     1561 caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg aaccggaagg
     1621 ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt cactggtatt
     1681 tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc acgtacggtt
     1741 ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg ttccgacagc
     1801 gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     1861 gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggcc ggcaaaccgg
     1921 gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     1981 tgacgagccg cttacttttg gcgtaattct gctgcgcctt tactgaacac aatgccgtat
     2041 tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga
     2101 cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt
     2161 atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg
     2221 caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc
     2281 ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta
     2341 ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt
     2401 ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg
     2461 attttctgtt cagacatggt atcccgctta cttttgttgt taaagtttat ccatcatggc
     2521 gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc
     2581 taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga
     2641 aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag ccaattaaaa
     2701 actggcgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt atcttttagt
     2761 acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct gccgaattca
     2821 tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact atcaaatttc
     2881 cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga agacatttcc
     2941 ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt gtcatcagca
     3001 gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga tatatcaccc
     3061 agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt cagctcagac
     3121 tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag ggcacgagtc
     3181 accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat accggcctct
     3241 ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga cgtctgcaaa
     3301 tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac aagtacacgt
     3361 aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa aatctcaata
     3421 agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaacgtat agttctggtg
     3481 atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac ctcttcaacc
     3541 acaaaagttt gagtatccac tttgtcatga tcaaccttga tctcagtaaa ttttgccttt
     3601 cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt attattcagg
     3661 atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc catcagtagt
     3721 tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct gctgccatac
     3781 gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca gatatcacgg
     3841 tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa acatcaagca
     3901 tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct gacttattcg
     3961 ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca tcctgttcaa
     4021 taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg aaatcaactt
     4081 gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt ttcaaaaatg
     4141 cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc agcttatcaa
     4201 taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc tcgcacagag
     4261 tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca taaacgccag
     4321 ggataactga cggaacgata aaatcttcca gtaattcctc acgactcacg ttctgcaaca
     4381 tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc agaaacatag
     4441 tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct gacagcaggt
     4501 gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta gacacaccgc
     4561 cttttagact accgatgaat atggaatatg cctcactata acgatcgcga tatttaggaa
     4621 tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat tttgtcgcgg
     4681 ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg actgcaccat
     4741 ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa tagacttgat
     4801 aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt agggcgagca
     4861 acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc attccccctc
     4921 cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg taatgcatgc
     4981 aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta ttaatcatcg
     5041 gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga tttcccccga
     5101 ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca gatggtgtat
     5161 ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca acaggcgggg
     5221 gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg actttttcgt
     5281 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
     5341 ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct
     5401 ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga
     5461 acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtgt
     5521 aagccaacac accgccagtt ttagtgttgt acgcatacca gagccagtgt t
//
LOCUS       DAJTMX010000200         5398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTMX010000200 DAJTMX010000000
VERSION     DAJTMX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.200"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..497
                     /locus_tag="OO145_003956"
     CDS             <1..497
                     /locus_tag="OO145_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5468884.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO145_003957"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO145_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO145_003958"
     CDS             2500..3114
                     /locus_tag="OO145_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5468885.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO145_003959"
     CDS             3114..3443
                     /locus_tag="OO145_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5468886.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO145_003960"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO145_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5468887.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO145_003961"
     CDS             4529..4840
                     /locus_tag="OO145_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5468888.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="OO145_003962"
     CDS             4824..4982
                     /locus_tag="OO145_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5468889.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="OO145_003963"
     CDS             4975..5373
                     /locus_tag="OO145_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5468890.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTMX010000201         5397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTMX010000201 DAJTMX010000000
VERSION     DAJTMX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.201"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="OO145_003964"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="OO145_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5468891.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..1725
                     /gene="tauB"
                     /locus_tag="OO145_003965"
     CDS             958..1725
                     /gene="tauB"
                     /locus_tag="OO145_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5468892.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVQLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1722..2549
                     /gene="tauC"
                     /locus_tag="OO145_003966"
     CDS             1722..2549
                     /gene="tauC"
                     /locus_tag="OO145_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5468893.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2546..3397
                     /gene="tauD"
                     /locus_tag="OO145_003967"
     CDS             2546..3397
                     /gene="tauD"
                     /locus_tag="OO145_003967"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5468894.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OO145_003968"
     CDS             complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OO145_003968"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5468895.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5001..5369
                     /locus_tag="OO145_003969"
                     /pseudo
     CDS             5001..5369
                     /locus_tag="OO145_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1258 a         1359 c         1490 g         1290 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttcacccgcg accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag
     1501 gtgcagttga ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg
     1561 ctttcatccg gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt
     1621 gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa
     1681 tatgttttaa gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg
     1741 aaaaactgca ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa
     1801 gcattggcac gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga
     1861 ttagcccgct gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg
     1921 gaccgcaggg atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca
     1981 ttgtgctggc gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc
     2041 taagcccgac ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac
     2101 cgctggctta tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct
     2161 tactgatcta tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa
     2221 gcgcgcagca ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc
     2281 tgtggtttgt cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc
     2341 tgggtgtggg ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag
     2401 gatttatggt tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg
     2461 cggtgattgc gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc
     2521 tgacgccctg gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc
     2581 gtatatcggc gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt
     2641 tgaacagctt taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat
     2701 tacgccgcag cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt
     2761 ttaccctcat gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc
     2821 gccggataac gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc
     2881 gattctggca gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat
     2941 tgcggcctat gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga
     3001 gcatgatttc cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca
     3061 acgctggcgc gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca
     3121 tccggtgagc ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga
     3181 tgtcagcgag aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc
     3241 ggagtttcag gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt
     3301 gactcagcac tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac
     3361 gatccttggg gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg
     3421 cccccttggg gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc
     3481 cccctttctg agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa
     3541 agtagctgaa aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga
     3601 ctttctcttc atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc
     3661 tcacctgata cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga
     3721 ggtacgctcc agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg
     3781 actcacgaat cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt
     3841 ttaatgcgct tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg
     3901 aatacgacat aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct
     3961 gtacctggcc gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa
     4021 ccacggcttg cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca
     4081 gcacaccgca gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca
     4141 tttctggcac agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc
     4201 aggcgtcgct gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga
     4261 taccggcgtt ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca
     4321 cgcctggcat ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca
     4381 acaccaggtc gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt
     4441 tgcgcaggcg acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat
     4501 taaggtgcac agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt
     4561 gacaagaaga gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc
     4621 atattcactg aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt
     4681 ttggttgtgc tgctataaat tgaataattc aaactttgtt gtatattgat aagatattta
     4741 tctggtttgt atttttgata tttataacgc gtatgcatca aaaataacat tatctgcaac
     4801 atactgaaat atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg
     4861 tccacattcg gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc
     4921 gttagcgcga aacataatat gttttctatg cattgataat tgatggatca acttattacg
     4981 tccctgagga gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag
     5041 cattggcttg cactctggct atcacctctc aggctaatgc agcgaccaac gatatttctg
     5101 gtcaaactta caatactttc catcactaca acgacgccac ctatgctgat gacgtttact
     5161 atgatggtta tgtaggctgg aacaactatg ccgctgatag ctattacaac ggcgatatct
     5221 acccggtcat taataacgct accgttaacg gcgtgatttc tacctactat ctggacgacg
     5281 gtatttctac caataccaac gccaatagtc tgacaatcaa aaacagcact attcacggta
     5341 tgatttattc cgagtgcatg acaactgatt agactggccc cctgaatctc cagacaa
//
LOCUS       DAJTMX010000202         5350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTMX010000202 DAJTMX010000000
VERSION     DAJTMX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.202"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(75..557)
                     /locus_tag="OO145_003970"
     CDS             complement(75..557)
                     /locus_tag="OO145_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468896.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="OO145_003971"
     CDS             complement(625..2574)
                     /locus_tag="OO145_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5468897.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="OO145_003972"
     CDS             complement(2659..2994)
                     /locus_tag="OO145_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5468898.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="OO145_003973"
     CDS             complement(2994..3350)
                     /locus_tag="OO145_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5468899.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="OO145_003974"
     CDS             complement(3350..4846)
                     /locus_tag="OO145_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5468900.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="OO145_003975"
     CDS             complement(4843..5004)
                     /locus_tag="OO145_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5468901.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="OO145_003976"
     CDS             complement(5014..>5350)
                     /locus_tag="OO145_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468902.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1277 a         1561 c         1149 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 agccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAJTMX010000203         5336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTMX010000203 DAJTMX010000000
VERSION     DAJTMX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.203"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO145_003977"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO145_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5468903.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO145_003978"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO145_003978"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5468904.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO145_003979"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO145_003979"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5468905.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHCKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO145_003980"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO145_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5468906.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1455 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acactgcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgg
//
LOCUS       DAJTMX010000204         5182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTMX010000204 DAJTMX010000000
VERSION     DAJTMX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.204"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..746
                     /locus_tag="OO145_003981"
     CDS             <1..746
                     /locus_tag="OO145_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5468907.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="OO145_003982"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="OO145_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5468908.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="OO145_003983"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="OO145_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5468909.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="OO145_003984"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="OO145_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5468910.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5182
                     /locus_tag="OO145_003985"
     CDS             5041..>5182
                     /locus_tag="OO145_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5468911.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1481 a         1102 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt ta
//
LOCUS       DAJTMX010000205         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTMX010000205 DAJTMX010000000
VERSION     DAJTMX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.205"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO145_003986"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO145_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5468912.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO145_003987"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO145_003987"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5468913.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO145_003988"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO145_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5468914.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO145_003989"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO145_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5468915.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO145_003990"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO145_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5468916.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1207 c         1256 g         1417 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacta aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTMX010000206         5021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTMX010000206 DAJTMX010000000
VERSION     DAJTMX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.206"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..345
                     /locus_tag="OO145_003991"
     CDS             <1..345
                     /locus_tag="OO145_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5468917.1"
                     /translation="WARRPSGTIHHSDKGSQYVSLAYKARLKEAKLLASTGSTGDSYD
                     NAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNDDLAA"
     gene            510..4628
                     /gene="pic"
                     /locus_tag="OO145_003992"
     CDS             510..4628
                     /gene="pic"
                     /locus_tag="OO145_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5468918.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4779..>5021)
                     /locus_tag="OO145_003993"
     CDS             complement(4779..>5021)
                     /locus_tag="OO145_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR5468919.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1355 a         1145 c         1333 g         1188 t
ORIGIN      
        1 tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca gtatgtgtca
       61 ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg gagtactggt
      121 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
      181 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      241 tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc agaagcagaa
      301 aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca cagataaaac
      361 actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca gtaactatat
      421 tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt tacttttata
      481 tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt aaatattgcc
      541 ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata aaaaagacat
      601 gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg tgttactctc
      661 agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat cgtgatttcg
      721 ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat gataaggacg
      781 gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt gtgagctcaa
      841 atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat aacggaggat
      901 atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac cgtaataacc
      961 acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa gttattccct
     1021 cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa cgttacaccg
     1081 ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga aatttagtta
     1141 aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct ctgatatctg
     1201 atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc cctttacctg
     1261 actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa caacaaaaaa
     1321 aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct acgaactggt
     1381 ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc gatgcccccg
     1441 tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca tcaggcacag
     1501 gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac aatgacctca
     1561 atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg aaagacagtg
     1621 tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct gctgaatccg
     1681 gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta acctggaagg
     1741 tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg accataaacg
     1801 gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta cttaaccagc
     1861 aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc agcggacgtc
     1921 cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca tggggatacc
     1981 ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg caggctgctg
     2041 attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta ctggatctta
     2101 aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc tttggtggta
     2161 ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc ttctatattc
     2221 tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat ccggctcagt
     2281 gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat aggatcctgg
     2341 ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat atggatgtcg
     2401 ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg aacctgccgg
     2461 aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg gttatccatg
     2521 cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa aaccgtcagt
     2581 ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt aacgcctcgc
     2641 tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac agggcatttg
     2701 tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc tctgtcccgg
     2761 acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat aactcagccc
     2821 tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc gtcagcatca
     2881 cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca ctgacagtgc
     2941 atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat attcaggccg
     3001 gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct aatcagtatg
     3061 cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca gcactggaaa
     3121 ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca acagttacca
     3181 tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg gcgtttgccg
     3241 gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt ggcagggctg
     3301 atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc cacagtctta
     3361 ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc ctgacggtga
     3421 ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa aatgccgata
     3481 aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc agctttatga
     3541 ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg gcgggaactt
     3601 cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg accccaaccc
     3661 tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt aaagcggagg
     3721 ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat aaaaacttca
     3781 tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca aacggtgatg
     3841 ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt tacagtgata
     3901 attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt gtggacctgt
     3961 ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca ttcagcggaa
     4021 agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc ggtgcctata
     4081 tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac tttgctagcc
     4141 tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg ggttaccgct
     4201 atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac ggcgccgtgt
     4261 ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag aacagggact
     4321 tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt ggtaaggact
     4381 ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat aatggagaga
     4441 ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac agccggatgc
     4501 tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt ctggagtttg
     4561 agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat ttccggtata
     4621 tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga ctcttaaaat
     4681 ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt tttgccgact
     4741 ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc gtccaggcgg
     4801 tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa cggcatcgtc
     4861 catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga gtcgtctgtc
     4921 gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga ctcgatggca
     4981 ttcgtcgtat agatcacttt gcggatgtcc gttggataag c
//
LOCUS       DAJTMX010000207         5020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTMX010000207 DAJTMX010000000
VERSION     DAJTMX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.207"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(684..1100)
                     /locus_tag="OO145_003994"
     CDS             complement(684..1100)
                     /locus_tag="OO145_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468920.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1127..1267
                     /locus_tag="OO145_003995"
                     /pseudo
     CDS             1127..1267
                     /locus_tag="OO145_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1267..2292
                     /locus_tag="OO145_003996"
     CDS             1267..2292
                     /locus_tag="OO145_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5468921.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2345..2434)
                     /locus_tag="OO145_003997"
     tRNA            complement(2345..2434)
                     /locus_tag="OO145_003997"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2398..2400),aa:Ser,seq:cga)
     gene            2528..3325
                     /gene="mtfA"
                     /locus_tag="OO145_003998"
     CDS             2528..3325
                     /gene="mtfA"
                     /locus_tag="OO145_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5468922.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3426..3501
                     /locus_tag="OO145_003999"
     tRNA            3426..3501
                     /locus_tag="OO145_003999"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3459..3461,aa:Asn,seq:gtt)
     gene            3663..4925
                     /locus_tag="OO145_004000"
                     /pseudo
     CDS             3663..4925
                     /locus_tag="OO145_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1387 a         1183 c         1124 g         1326 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg
     1141 gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg
     1201 ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc
     1261 gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac
     1321 ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc
     1381 atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat
     1441 cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac
     1501 ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc
     1561 ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga
     1621 ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt
     1681 tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag
     1741 gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca
     1801 cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat
     1861 gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa
     1921 acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca
     1981 aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac
     2041 aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt
     2101 ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc
     2161 agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac
     2221 aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa
     2281 tattccaagt aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga
     2341 attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg
     2401 agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg
     2461 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
     2521 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
     2581 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa
     2641 attggtcgct cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt
     2701 tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
     2761 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
     2821 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
     2881 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
     2941 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
     3001 cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca
     3061 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc
     3121 ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     3181 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     3241 ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     3301 gttttcagcg acgaatgttc attaattaac aactttgaag attaattaac caattgaaat
     3361 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     3421 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     3481 cgagtccagt cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga
     3541 tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc
     3601 atcgggaaaa attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc
     3661 atatatccct taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag
     3721 tctccgattc tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc
     3781 tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct
     3841 ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca
     3901 acccggtaca gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa
     3961 acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc
     4021 tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac
     4081 ttaaaccccg tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg
     4141 ttgcgtcccg cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt
     4201 taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc
     4261 actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc
     4321 atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct
     4381 ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga
     4441 tacccgcgac gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc
     4501 gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg
     4561 atatcaccgg taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt
     4621 gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct
     4681 gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa
     4741 aggacgcagt agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata
     4801 ttcataaggc agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc
     4861 tggaagcatg ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc
     4921 catagtatac taacgaatcc cacactctgg caaattacca ccacctctac aggaggtggt
     4981 tctatataga cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAJTMX010000208         4973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTMX010000208 DAJTMX010000000
VERSION     DAJTMX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.208"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO145_004001"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO145_004001"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5468923.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO145_004002"
     CDS             1020..1703
                     /locus_tag="OO145_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5468924.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO145_004003"
     CDS             1700..2161
                     /locus_tag="OO145_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5468925.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO145_004004"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO145_004004"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5468926.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO145_004005"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO145_004005"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5468927.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO145_004006"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO145_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5468928.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO145_004007"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO145_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5468929.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctaggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAJTMX010000209         4904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTMX010000209 DAJTMX010000000
VERSION     DAJTMX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.209"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            245..429
                     /locus_tag="OO145_004008"
                     /pseudo
     CDS             245..429
                     /locus_tag="OO145_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            572..793
                     /locus_tag="OO145_004009"
                     /pseudo
     CDS             572..793
                     /locus_tag="OO145_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(814..876)
                     /locus_tag="OO145_004010"
                     /pseudo
     CDS             complement(814..876)
                     /locus_tag="OO145_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1326..2738
                     /gene="ospC1"
                     /locus_tag="OO145_004011"
     CDS             1326..2738
                     /gene="ospC1"
                     /locus_tag="OO145_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5468930.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2842..2991)
                     /locus_tag="OO145_004012"
                     /pseudo
     CDS             complement(2842..2991)
                     /locus_tag="OO145_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3067..4764
                     /gene="ospD3"
                     /locus_tag="OO145_004013"
     CDS             3067..4764
                     /gene="ospD3"
                     /locus_tag="OO145_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5468931.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1691 a          891 c          922 g         1400 t
ORIGIN      
        1 tctccaggat tcccggggcg gttcaatcaa agcgcagaac ttctgcgtct tcttcgtcaa
       61 aaaaatcatc ttcgtgaagc ttcacgacat agcggggaag tttgcttctt tgagaggcgg
      121 gtttacgttt acgggattta gctgaacggg ccatataacc acctgaaaga caatgacatt
      181 gcctgttttt ataacggtaa ttgcagacca tgacaaaccg cagccgtcag gctgcctact
      241 cgagtcacaa ggctctttgt gtccgatatg ccggcagcgc attacccatg aaactggctg
      301 gaacgtacac cataaggtga agaaagttat gggggcggtg aagaattatc taaccttgtc
      361 ttgttacatc caaattgcca tcgacagtta cacagttgtg aagccggctc tctcaaaaag
      421 ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat gcaccgttct taggggaggg
      481 cagtccagca atgaactgtc cttacccgac aaacatccaa aacgaacgga acgtgctgga
      541 atccgcatat aacccgttgt aaagtgagga aatggacacc tcacttgctc atgagaacgc
      601 ccgcctgcgg gcactgttgc agacgcaaca ggacactatc caccagatgg ctgaatacaa
      661 ccgcctgctc tcacagcgga tggcggctta tgcttccgaa atcaaccgac tgaaggcgct
      721 ggttgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc gcgcaaaaac
      781 cgaacggcag attccatttt cacgggcgat ctacgccaca caggctctgg gttgcagtga
      841 ctgctccatt aaggcgattt tgaactcata agggaagtaa caaaaaaact gcaaaagtac
      901 aataattata tatggccaaa ctaacctcta ttaaaaaaca tcagtttttc acacttagtt
      961 gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca ctaactggaa
     1021 gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt cattgctgtt
     1081 caataaaatt gatatagtta ttaaattatg aaaataattt cacaaaaaag attaacttga
     1141 gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa catcaaaagt
     1201 atataatact cttatctaat tacatgcagt atattgtata tatactcaaa aattgtaatt
     1261 cataccctgc tatatcttgt agggaaagat attaattaaa actgttttca tataaggttc
     1321 attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat atagattcta
     1381 tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac
     1441 aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa agtttcttta
     1501 ggaaaactat agcagcacag agttatagta gaatgttctc tcaaggctct aactttaaat
     1561 ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag gctattgagc
     1621 accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt catcctcttt
     1681 ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc ttcagacatc
     1741 aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat aaaattcagt
     1801 ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa atagctaatc
     1861 atgactttgt gttttttggc gttgaaatct ctaaccatca gaaaaaacac cccctgaata
     1921 caaaacatca cactgttgat tttggtgcaa atgcgtatat tattgatcat gactctccat
     1981 atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt ttttaccatg
     2041 agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt cgatacgtac
     2101 atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg aagttagggc
     2161 tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc aaggagtttt
     2221 gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt gtttttcagc
     2281 cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt aagattagag
     2341 aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac aagcaggtca
     2401 ctaacaaaaa aattgctctc caggctcttt ttctttcgat tactaatcaa aaagaggatg
     2461 tcgccttata tatattatct aattttgaga taactagaca agatgttatt tccataaagc
     2521 atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt aaagtacttg
     2581 agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa actaatagtg
     2641 gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata aaactattat
     2701 tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat atcgtttagc
     2761 aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg gctcaatgtg
     2821 ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga actggaaggt
     2881 cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc cgttccggat
     2941 acccccctga gtttcagagt aacggtgaca aaccacatca atctttgcca tcaatcatcc
     3001 aaagggcaaa gagtacaaca acactaagtc tgcgtcacaa cccatcaatg aaaggaatat
     3061 atacatatgc catcagtaaa tttaatccca tcaaggaaaa tatgtttgca aaatatgata
     3121 aataaagaca acgtctctgt tgagacaatc cagtctctat tgcactcaaa acaattgcca
     3181 tatttttctg acaagaggag ttttttatta aatctaaatt gccaagttac cgatcactct
     3241 ggaagactta ttgtctgtcg acatttagct tcctactgga tagcacagtt taacaaaagt
     3301 agtggtcacg tggattatca tcactttgct tttccggatg aaattaaaaa ttatgtttca
     3361 gtgagtgaag aagaaaaggc tattaatgtg cctgctatta tttattttgt tgaaaacggt
     3421 tcatggggag atattatttt ttatattttc aatgaaatga tttttcattc cgaaaaaagc
     3481 agagcactag aaataagtac atcaaatcac aatatggcat taggcttgaa gattaaagaa
     3541 actaaaaatg ggggggattt tgtcattcag ctttatgatc ccaaccatac agcaactcat
     3601 ttacgagcag agtttaacaa atttaactta gctaaaataa aaaaactgac tgtagataat
     3661 tttcttgatg aaaaacatca gaaatgttat ggtcttatat ccgacggtat gtctatattt
     3721 gtggacagac atactccaac aagcatgtcc tccataatca gatggcctga taatttactt
     3781 caccccaaag ttatttatca cgcgatgcgt atgggattga ctgagctaat ccaaaaagta
     3841 acaagagtcg tacaactatc tgacctttca gacaatacgt tagaattact tttggcagcc
     3901 aaaaatgacg atggtttgtc aggattgctt ttagctttac aaaatgggca ttcagataca
     3961 atcttagcat acggagaact cttggaaact tctggactta accttgataa aacggtagaa
     4021 ctactaactg cggaaggaat gggaggacga atatcgggtt tatcccaagc acttcaaaat
     4081 gggcatgcag aaactatcaa aacatacgga aggcttctca agaagagagc aataaatatc
     4141 gaatacaata agctgaaaaa tttgctgacc gcttattatt atgatgaagt acacagacag
     4201 atacccggac taatgtttgc tcttcaaaat ggacatgcag atgctatacg cgcatacggt
     4261 gagctcattc ttagcccccc tctcctcaac tcagaggata ttgtaaattt gctggcctca
     4321 aggagatatg acaatgttcc cggacttctg ttagcattga ataatggaca ggctgatgca
     4381 atcttagctt atggtgatat cttgaatgag gcaaaactta acttggataa aaaagcagag
     4441 ctgttagaag cgaaagattc taatggttta tctggattgt ttgtagcctt gcataatgga
     4501 tgtgtagaaa caattattgc ttatgggaaa atacttcaca ctgcagacct tactccacat
     4561 caggcatcaa aattactggc agcagaaggc ccaaatgggg tatctggatt aattatagct
     4621 tttcaaaata ggaattttga ggcaataaaa acttatatgg aaataataaa aaatgaaaat
     4681 attacacctg aagaaatagc agaacacttg gacaaaaaaa atggaagtga ttttctagaa
     4741 attatgaaga atataaaaag ctgaatatta tctcgtggtg atacactgaa tttgaggtaa
     4801 ccggctcatt taaaccgtct ggtctgttac ctccggttcc acaaaaataa tgtccatcat
     4861 tttgaagtgg acactatcgt atggaatacc ggacctggat cact
//
LOCUS       DAJTMX010000210         4843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTMX010000210 DAJTMX010000000
VERSION     DAJTMX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.210"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            81..350
                     /locus_tag="OO145_004014"
     CDS             81..350
                     /locus_tag="OO145_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5468932.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OO145_004015"
     CDS             344..709
                     /locus_tag="OO145_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5468933.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO145_004016"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO145_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5468934.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OO145_004017"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OO145_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5468935.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OO145_004018"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OO145_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5468936.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OO145_004019"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OO145_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5468937.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..>4843)
                     /locus_tag="OO145_004020"
     CDS             complement(4575..>4843)
                     /locus_tag="OO145_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5468938.1"
                     /translation="KASLMSMTPTLNRGLQRYIADSNSALLGLQPEDWLDMAEPVNIP
                     GTSYQYKNWRRKLSATLESMFADDGVNKLLKDLDRRRRAAAKKK"
BASE COUNT         1116 a         1296 c         1238 g         1193 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgttggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tat
//
LOCUS       DAJTMX010000211         4779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTMX010000211 DAJTMX010000000
VERSION     DAJTMX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.211"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO145_004021"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO145_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5468939.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO145_004022"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO145_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5468940.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO145_004023"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO145_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5468941.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO145_004024"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO145_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5468942.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4144..>4779)
                     /locus_tag="OO145_004025"
     CDS             complement(4144..>4779)
                     /locus_tag="OO145_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5468943.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1267 a         1168 c         1127 g         1217 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataaccta
     3781 tacaaaatga aaataattat cattaacagc tttgaggcct aaaacaggga ttaggttgtt
     3841 tcctgctggc gacatgatat tgaggaaaaa agtagagcag ctattaggct ttgaaatgac
     3901 tttatgagga aaattgttga tggcaaaaat ggggcaaaaa agagtgggtt atcgtagctt
     3961 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgagtcaga
     4021 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttctttg taatttgttg
     4081 attttcttgc attatttcag ttctctggta ctaaatgggg caaattgggg gcaaactttg
     4141 caactacgat aaccgcgcat tcaacatggc tatctgttcg tcgttcatgt catcaatcca
     4201 cataccgtaa atttcataca ccatttgcgc agtttcatgc cccatctggc tggctataaa
     4261 tgccgggttc gctcctgccg tcaacagcca gcaggcaaaa gtatgtcgcg tatggtacgg
     4321 attacggcgg cgaataccag cacgttttac tgctgcattc catcttgtac ccaaactgct
     4381 taccgagtaa taaggtttct gtttttcgtt acacaccctg ggcatgaaaa caaaatgcag
     4441 tttttgtttt tcagttctgc cgtactcccg atgataaaaa gtgatttcgc ttttgcgatg
     4501 atgcccggtc agtttgtatt gctccttcag tgcttcaaga gccggctgca gtaatgctac
     4561 cgtccggatc ccggcatttg tttttggggg accgaacata tcaagtatcg tcaggtttct
     4621 tctgacattc acaattccct tctcgaaatc cacatcctcc cacgccagag ctgccagttc
     4681 cccgtgacga agcccggagt aaacggcaaa tttccacaag ttttggctct gtcctttttc
     4741 actttccatt aatgcattga attctgtttt agataacgg
//
LOCUS       DAJTMX010000212         4758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTMX010000212 DAJTMX010000000
VERSION     DAJTMX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.212"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..164
                     /locus_tag="OO145_004026"
     CDS             <1..164
                     /locus_tag="OO145_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5468944.1"
                     /translation="VFGNPVRGYPSMLVSILFLGGVQLIGIGVLGEYIGRIYLETKSR
                     PRYLIKSRK"
     gene            177..1442
                     /locus_tag="OO145_004027"
     CDS             177..1442
                     /locus_tag="OO145_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5468945.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(1509..2132)
                     /locus_tag="OO145_004028"
     CDS             complement(1509..2132)
                     /locus_tag="OO145_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5468946.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTAILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(2850..3221)
                     /locus_tag="OO145_004029"
                     /pseudo
     CDS             complement(2850..3221)
                     /locus_tag="OO145_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            3343..3603
                     /locus_tag="OO145_004030"
     CDS             3343..3603
                     /locus_tag="OO145_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR5468947.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(3621..>4758)
                     /locus_tag="OO145_004031"
     CDS             complement(3621..>4758)
                     /locus_tag="OO145_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468948.1"
                     /translation="VEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNLDPIEKRVFRAV
                     NPGKNGALTYANLNKQRRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALSETN
                     ALRDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRELMQ
                     NTPSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLPKET
                     MSGLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAVGST
                     VSGPFSAVGAVAGSEIGTKVASKIRAMGGAESIESAEKLISSPEFQKAARLAVKQAPE
                     SIVDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
BASE COUNT         1328 a         1051 c          917 g         1462 t
ORIGIN      
        1 ttgtctttgg taacccagta cgcgggtatc cctccatgct tgtatcaata cttttcctgg
       61 gtggagtgca actgatcggg attggtgttc tcggtgaata tataggtaga atttatttag
      121 aaacaaaatc aagaccacgg tacttaatta aaagcagaaa ataacaggat gatcttatga
      181 agatattcat aatgcgcaat tggcacaaga tatcaatttt tatacttgca tttactttga
      241 tttggcttag gcgcattgac attttgacaa atgcccagtt ttgggctgag gatgcagttt
      301 tttggtataa agatgcatac gaacaaggtt ttttcagttc actcataacc ccaagaaatg
      361 gttattttca aacagtatca acactcattg ttggtgcgac gacatttatt aatccaattt
      421 acgcaccatt attatcaaac ttttttggaa taataattag agctataatt atatggttct
      481 tatttaccga tagatttaaa ttcctaagca ccacatcaaa aatcttcatc tctgtatatt
      541 taatttgcat gcccgggctt gatgaagtcc aagccaatat aacaaatgct cactggtatt
      601 tatcattgta tgttgcaatg ataataatat ctgaagaatc caagtcaaaa ttatggaaag
      661 cacatgattt attttttata gtattatctg gattgagcgg cccatttatt atattcataa
      721 ttgcatcatc tgtttttaaa tatttacact tatcaaaagg agtaataagc ataagaggtt
      781 tatacttatt ttatactcga ctgccatatt tggcaatgat cttgtgtggg ctgatacagg
      841 caacatcaat aattcttaca tttaatggaa caagatcaca tgctccatta ggattcagtt
      901 ttgatgttat gtcatctata atatcatcta atgtatttct tttttcattt gtcccttgga
      961 atatcgctaa tgctggatgg gataatcacc ttttatcgta cacactatca actttagtaa
     1021 ttacattatg tgttttcatt tatattaaag gaaattggca aatgaaagtg tttgccaccc
     1081 ttccaatact tattgtcgca ttttctatgg caaaacctca actggcagat tctgttcctc
     1141 aactgccaac acttgcaacc ggaaacgggt caaggtattt tgtaaatatt catattgcaa
     1201 ttttttcatt aatatgcgtt tgtttgtttc aatgcataaa gaataaaacc ttaaaaatat
     1261 tctttaaaat ctatgtttca gtcatcctta ttgcaatgat aaaattaaac ttttttataa
     1321 cccctcttcc tgacatgaac tggtctcaag gagcagaatt aataaataaa gcaaaacatg
     1381 gagaagccgt ctcatttaca gtgctccctc catggttaac tcttgattta ataaaaaaat
     1441 aaaacttcac attaataaat aacaatgaat ccgcctccct acgtattccc gtagggaggc
     1501 tgtcgtgttt atagtagcct ggtaatgcta aatgcaaacg aactggagat tcctgctccg
     1561 ctaagcgtat tgcgcagggt caccctaaat gtatctacgc caatataagt aacatcacaa
     1621 ctaactgaat caggcgcaga tataggtgtt atctgcacaa tataaccgct agcaactgct
     1681 gcgagcggat gagaaaagct aacctcatac acacctgtag caaccacgtt aaccgatgaa
     1741 acaccagtag caacgtcgat actaccattg gcgttaattt ttcccgagcg tgtgactgac
     1801 aatgcagaag ccgatgttgc cttactggtt gtttctgtaa aaacacaccc aatgtcaact
     1861 acctcgcaat tagctcctgt tatccagtat ggtcgatctg cacttggctg ataatcccct
     1921 gcgctctgga acgttgtgcc gttgagtatg gctgtaactt ttcccccacc tgagcttgtt
     1981 acttgaatat tagtgtgcgt gtatcttgca ctgctcacgc gatgaaaatt tccaccgttt
     2041 ataaccacag ttacagggcg cgtcccagta ttatctatag caaggtctgc ccccccggca
     2101 ttagcctcaa agtatgggtt gtttaatacc aaagcgcacg aaccgttaag gccatttatg
     2161 gctaattgcg caccacctgt actggaatcc ccctgagtac catttccttc acaagtcagg
     2221 ctattgatag ttgtaccagc accccagcgg tccccgagga taccccattc actgttctga
     2281 ctgcacgtaa gcctatttgc agttatagcg ttcagttcag aatgatttac ggagttggta
     2341 gcacgccaac cgatacgatt actctgcaag tacacgttcg tcatgtcgct cgtgagcatc
     2401 gagtcagtgc gtaacccttc cccgtcaggg tggcggcaga taacaatgtt ttcaagacga
     2461 gtatgtgctt tattctgcac cagcataccg taacttccgg gattcccttt cagcgtcatg
     2521 ttgccgatgt agtcatatgc agccaatctc tgactgttaa acgctgcgtc acctagcatt
     2581 tttacgttaa aatcagtagc tctacattcc agtattgtct cagccatgtt ctcgcctgag
     2641 taaaaacagc ggacacccgg agcacctaca aagtcggcag ggtaatcatc ataattaagt
     2701 accagaggag tggtaacatc atacacccct tgctttattt ttaatgttcc accgaaaggc
     2761 cacgatgtaa gcgggtcttg atatatttca ttttttattg caaatagctc atcttgaaga
     2821 tttttcccgc tattagtgcc aatgtaagta gccccttcat cagattgaag taactgcaga
     2881 agctgttcag ggtcatactt cagcaaattc ggaaaataga actgttgcga accatacgca
     2941 tcatatacag ccattgaatg gccttgctca gttacaaatt tggcaatctg cccgttgtac
     3001 acaggatatc cggcagcatt gataacaatt ggctgagaaa cagggacgtg agatccgtct
     3061 tcattctcta cataaacctg aatctggttt tcagtattta ccgggtcagt gtcaattttt
     3121 ccgatataaa ttttgccatt ggctacagct ttaaaagaac gcgccatagt gaagagttgc
     3181 gaaggcatgc ttaccacaac atttgcggtg atatctgaca tttcattgct ccagacgaat
     3241 gatatgatgc aatcatgata tgattgcata ccgaaatggt actattgagt atttatccag
     3301 taggttacga tgccattcca cccaactggt gaggcatcaa ggatgtacag caaatacgac
     3361 gaggcgcagt ttcacttgag acttccgcat gaactccacg cgaaaattaa acagcgcgcg
     3421 aagatgaata acaggtcgct gaactcagag ataattgcag cgattgaaga atcattggct
     3481 aaacaaagct ctgcatcagt ttacattgac gatgcagagc gtatggcaga acaacaatct
     3541 gatatggtta agaaaattgt ctttgatacg ctcaaggagc tatataaaaa agacagcaac
     3601 taaccatccg tggctttccg ttacttctgg ttatcatcac tgttcatcca gtacatgatg
     3661 cctagccttg atatggtttt cttatctgat tctggaagtg agttgtaaaa cgagcgccaa
     3721 gcagaagagc gtcttacagt tgtatcaacg atgctttctg gtgcttgttt tactgccagc
     3781 cttgctgctt tctggaattc tggtgagcta attagctttt ctgcagattc aattgattca
     3841 gcgccgccca tcgccctgat tttgctggcg acttttgtcc caatttctga cccagcaaca
     3901 gcacctactg cactgaacgg tcctgacaca gttgaaccaa ccgcagtgcc aatgcgttgg
     3961 gcatggttag caacaaattc atttgccgca gtgacgcgat taaaccgttt gacgaactcg
     4021 tttagtcttc ctgtagttat ctcgtaagat tttgcgtctt ttatagcctt tgcgacctta
     4081 tatacatcgt tcagacctga catagtctct tttggtaaat gtcgggcaag attgcgcatc
     4141 tgcccgtttg ctaacatgtt ttgataccag tcagcaaacc ctgcaggatt aaaatcagcg
     4201 ccacgttttc cgttcgaaag catatcccga agacctgttc ccagtagctc ggttcttagc
     4261 ttcctggacg gcgtgttttg catcaattca cgaaatcctt tggcatcgcc tttcgacatt
     4321 gcctgtagtg ctgtagttgc tttgcgagaa acatcaccgt tcagcgttct gccagttaga
     4381 ttaatcattt gctcttccat gctttttcgc atctgaacaa gcctctgagc aacttcaaaa
     4441 tcacgtaatg catttgtctc tgacagcgcc gccttttgat catcggcgag cgaaccgtaa
     4501 agcctcgata aagcagcgcg atcagcatct ttatatggtc cagaattctt atgaagtgca
     4561 tcaccaacaa gtcgtcgttg cttattgaga tttgcataag ttaatgcgcc gttcttgcct
     4621 ggattaactg cccgaaatac tctcttttca atagggtcaa ggtttttcat cccgccaaga
     4681 tcttctgcca cctgtttgag cattgacctt gttgatggcg catcaatttt tgcacctgca
     4741 ggcatagcct tttcaaca
//
LOCUS       DAJTMX010000213         4663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTMX010000213 DAJTMX010000000
VERSION     DAJTMX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.213"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(337..582)
                     /locus_tag="OO145_004032"
     CDS             complement(337..582)
                     /locus_tag="OO145_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468949.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="OO145_004033"
     CDS             complement(1359..2201)
                     /locus_tag="OO145_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5468950.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="OO145_004034"
     CDS             complement(2286..2483)
                     /locus_tag="OO145_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468951.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="OO145_004035"
     CDS             complement(2503..2991)
                     /locus_tag="OO145_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468952.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="OO145_004036"
     CDS             complement(2988..3365)
                     /locus_tag="OO145_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5468953.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="OO145_004037"
     CDS             complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="OO145_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5468954.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="OO145_004038"
     CDS             complement(3869..4090)
                     /locus_tag="OO145_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5468955.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /locus_tag="OO145_004039"
     CDS             complement(4177..4653)
                     /locus_tag="OO145_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5468956.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         1090 a         1116 c         1228 g         1229 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aggctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gcc
//
LOCUS       DAJTMX010000214         4636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTMX010000214 DAJTMX010000000
VERSION     DAJTMX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.214"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..311
                     /locus_tag="OO145_004040"
     CDS             <1..311
                     /locus_tag="OO145_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5468957.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO145_004041"
     CDS             468..1217
                     /locus_tag="OO145_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5468958.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO145_004042"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO145_004042"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5468959.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO145_004043"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO145_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5468960.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO145_004044"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO145_004044"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5468961.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="OO145_004045"
     CDS             4084..4557
                     /locus_tag="OO145_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5468962.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1137 g         1098 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAJTMX010000215         4631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTMX010000215 DAJTMX010000000
VERSION     DAJTMX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.215"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..370
                     /gene="bglB"
                     /locus_tag="OO145_004046"
     CDS             <1..370
                     /gene="bglB"
                     /locus_tag="OO145_004046"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5468963.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            456..2072
                     /gene="bglH"
                     /locus_tag="OO145_004047"
     CDS             456..2072
                     /gene="bglH"
                     /locus_tag="OO145_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5468964.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2099..3274
                     /locus_tag="OO145_004048"
     CDS             2099..3274
                     /locus_tag="OO145_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5468965.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3276..3998
                     /locus_tag="OO145_004049"
     CDS             3276..3998
                     /locus_tag="OO145_004049"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5468966.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4060..>4631)
                     /locus_tag="OO145_004050"
     CDS             complement(4060..>4631)
                     /locus_tag="OO145_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5468967.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1285 a         1026 c         1130 g         1190 t
ORIGIN      
        1 acgggttctg ttaaatacgc tttgggatcg ttatcaaaaa ccgttattta ttgtcgagaa
       61 tggattaggc gtaaaagaca gcgttgaagc ggatggttcg atacaggacg attatcgaat
      121 tgcctattta aacgatcacc tggtacaggt aaatgaagcg attgccgatg gtgtggatat
      181 tatggggtac accagttggg ggccaattga tttagtcagt gcatctcatt cacaaatgtc
      241 taagcgctac ggctttattt atgtggatcg tgatgataat ggcgaaggaa gcctcacaag
      301 aacgcgtaag aaaagcttcg gatggtatgc agaagtgatc aaaacgcggg ggctgtcatt
      361 aaaaaaataa ccaataaagc accttaatta tcgtcgcatt cagaacagtc tggatgcgat
      421 acgttaattc tttctttgca ccataaaggg atattatgtt tagacaaaat cttattacct
      481 ctgccatctt attaatggca ccgttagcct tttctgcaca atcattggct gaatcattaa
      541 cggtggaaca acgccttgag ttattagaaa aagcgttaag agaaacgcaa agcgaactca
      601 aaaagtataa agatgaagag aagaaaaaat atacgccagc gacggtgaat cgtagcgtaa
      661 gtacgaatga tcaagggtat gccgccaatc cgttcccgac cagtagtgcc gcaaaacctg
      721 atgctgtact ggtcaaaaat gaagagaaaa atgccagtga gacaggctcg atttattctt
      781 ccatgactct gaaagatttc agtaagtttg tgaaagatga aattggcttt agttacaacg
      841 gctattaccg ttctggttgg gggaccgcct ctcatggttc acctaaatca tgggcgattg
      901 gttctctggg ccgctttggt aacgaatact ccggctggtt tgatttgcag ttaaaacaac
      961 gtgtctacaa cgaaaacggc aaacgggttg atgccgttgt gatgatagat ggtaacgttg
     1021 gtcagcagta ctctaccggc tggtttggcg ataatgccgg tggcgagaac tttatgcagt
     1081 tctccgatat gtacgttacc accaaaggtt tcctgccctt tgcgccagag gctgatttct
     1141 gggtgggtaa acacggtgcg ccgaaaattg aaatccagat gctggactgg aaaacgcagc
     1201 gtactgatgc tgcagcgggt gtaggtctgg aaaactggaa agtcggtccg ggtaaaattg
     1261 atatcgcgct ggttcgcgaa gatgtcgatg attacgatcg cagcctgcaa aacaaacagc
     1321 agattaatac ccatactatt gatttacgct ataaagatat cccgttatgg gataaagcga
     1381 ccttaatggt gagtggtcgt tatgtcacgg caaacgaaag cgcatcggaa aaagataatc
     1441 aggataataa cgggtattat gactggaaag atacctggat gttcggcaca tctttaacgc
     1501 agaaatttga taaaggtggc ttcaacgaat tctccttcct ggttgcgaat aactctatcg
     1561 ccaggaactt tggccgttat gctggcgcaa gtccatttac cacctttaat ggtcgttatt
     1621 atggtgatca caccggcgga acagcagttc gtctgacctc gcagggcgaa gcctatatcg
     1681 gcgatcattt tattgtggct aacgcgattg tttactcctt cggtaacaat atttatagct
     1741 acgaaacagg cgcacactct gatttcgaat ctattcgtgc ggttgttcgc ccggcctata
     1801 tttgggacca atataaccag acaggtgttg aactgggcta tttcacccag caaaacaaag
     1861 atgcgaatag taataaattt aatgagtctg gttataaaac cacgctcttc cataccttta
     1921 aagtcaatac cagtatgttg acctcgcgtc tggaaattcg tttctacgcc acgtatatca
     1981 aagccctgga aaacgaactg gatggcttca ccttcgaaga caataaagac gcccagtttg
     2041 ctgtcggtgc ccaggctgaa atctggtggt aatggaactt aatgaaagga atattacgat
     2101 gaatataaaa attgctgctt taacgctggc tattgccagc ggtatttctg ctcagtgggc
     2161 catagcagcg gatatgccag ccagcccggc acccactatc ccggtgaaac agtatgtgac
     2221 tcaggtcaat gccgataaca gcgtgacctt tcgctacttt gcccctgggg caaaaaatgt
     2281 ctctgtagtg gtgggtgttc cggttccgga caatattcac ccgatgacca aagacgaagc
     2341 aggagtctgg tcgtggcgca cacctatcct gaaaggcaac ctgtacgagt attttttcaa
     2401 tgttgatggt gtacgcagca ttgatacagg caccgcaatg actaagcctc agcgccaggt
     2461 taactccagt atgattctgg tgccaggcag ttatctggat acgcgttctg ttgcgcatgg
     2521 tgatttgatc gccataactt accactccaa cgcattgcaa tctgaacgtc agatgtatgt
     2581 ctggaccccg ccaggataca ccggcatggg cgagcctttg ccagtgctct atttctatca
     2641 cggttttggt gataccggac gttccgctat cgatcagggg cgtatcccgc aaatcatgga
     2701 taacctgctg gctgaaggga aaattaaacc gatgctggtg gtgatcccgg ataccgaaac
     2761 cgatgcgaag ggcattattc ccgaagattt cgtgcctcag gaaagacgta aagtctttta
     2821 tccgctgaat gctaaagcgg cagatcgcga actgatgaac gatattatcc cgctgattag
     2881 caagcgtttt agtgtccgta aagatgccga tggtcgcgcg ctggcagggc tttcacaagg
     2941 cgggtaccag gcgctggttt ccggaatgaa tcatctggaa agctttggct ggctggccac
     3001 attcagtggt gttaccacga caaccgtacc ggatgaaggt gtcgcggccc ggctgaacga
     3061 tccggcagct attaaccagc aactacgtaa ttttactgtg gtcgttggtg ataaagatgt
     3121 cgtaaccggc aaggatatcg ccgggctgaa aactgagctt gagcagaaaa aaattaagtt
     3181 tgattaccag gaatatcccg gcctgaacca tgaaatggat gtctggcggc ctgcctatgc
     3241 agccttcgta cagaaattta aaataaggca ttaagatgaa attaatcatt accgaagatt
     3301 accaggaaat gagccgtgtc gcggcacacc atctgcttgg ttatatgtcg aagacgcgtc
     3361 gtgttaacct ggcaattacc gccggtagca cgcccaaagg catgtatgaa tacctcacca
     3421 ccctggttaa aggtaagcct tggtacgata actgctattt ctataatttc gatgaaattc
     3481 cctttcgcgg caaagaggga gaaggcgtaa cgattaccaa tctgcgtaat ctgtttttca
     3541 cccctgcggg gatcaaagaa gagaatatcc agaagctcac tattgataac taccgcgagc
     3601 atgatcagaa actggcgcgt gaaggcggcc tggatttggt ggtgctggga ttaggtgcag
     3661 atggtcattt ttgtggcaat ttaccgaata cgacccattt ccatgagcaa accgtggagt
     3721 tcccgatcca gggagagatg gttgatattg tggcgcatgg tgagttgggc ggcgactttt
     3781 cgttagtgcc tgacagctac gtgacgatgg ggccgaaaag catcatggcg gcgaaaaact
     3841 tgctgataat tgtcagtggc gcaggtaaag cgcaggcact gaaaaatgtg ctgcaaggtc
     3901 cggttaccga agatgttcca gcttccgtgt tgcagctgca tccttcgctg atggtaatcg
     3961 ctgataaagc cgctgccgca gaattagcgt tcggttaagc tgaagaaata aggccggatg
     4021 tatgaattac gctccggcct gttctcatta tttaaataac tacgaaaaat caccccacag
     4081 tctcagcttg ccgcagtgga gacagcggaa gagataaccc tgacaatcac cacccttttg
     4141 caggcattta gctagatcag aatttctaat accgaaattc tcacaatctt cttcaaggtt
     4201 ggcaaattca tcgaggcgat cttttatatc attccagccc acatagccga taaaagcaca
     4261 gaaatcgcca caatgcgcaa gccagaattc ctgttgccat ccatgataac ctggcgtgcg
     4321 ttcaaccaac tctttcagca tttcatcagg atatgtgttc ttaataccgg caaattcgtc
     4381 ctcttcatca tactcaaatt caacaccttc tatgctggca tcatcctgaa aactacctgc
     4441 aaacttttct gcagcagaac cgtccgcaat acacaacgga cagagatgtt caacttcatc
     4501 aacgcaataa aagggacccg agtaataaac tgacgtctgt tgttcacagc aatcgcactc
     4561 tacggtttta tcctgttcaa atgcgcctgt ttccagtggc ttgggatgat atttgaatgt
     4621 aagcgccccg a
//
LOCUS       DAJTMX010000216         4603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTMX010000216 DAJTMX010000000
VERSION     DAJTMX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.216"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO145_004051"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO145_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5468968.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="OO145_004052"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="OO145_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5468969.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="OO145_004053"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="OO145_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5468970.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /locus_tag="OO145_004054"
     CDS             complement(3930..>4603)
                     /locus_tag="OO145_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5468971.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAJTMX010000217         4587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTMX010000217 DAJTMX010000000
VERSION     DAJTMX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.217"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..587)
                     /locus_tag="OO145_004055"
     CDS             complement(<1..587)
                     /locus_tag="OO145_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR5468972.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFL"
     gene            complement(702..777)
                     /locus_tag="OO145_004056"
     tRNA            complement(702..777)
                     /locus_tag="OO145_004056"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(742..744),aa:Thr,seq:ggt)
     gene            complement(784..858)
                     /locus_tag="OO145_004057"
     tRNA            complement(784..858)
                     /locus_tag="OO145_004057"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Gly,seq:tcc)
     gene            complement(975..1059)
                     /locus_tag="OO145_004058"
     tRNA            complement(975..1059)
                     /locus_tag="OO145_004058"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1023..1025),aa:Tyr,seq:gta)
     gene            complement(1068..1143)
                     /locus_tag="OO145_004059"
     tRNA            complement(1068..1143)
                     /locus_tag="OO145_004059"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1108..1110),aa:Thr,seq:tgt)
     gene            1505..2455
                     /gene="coaA"
                     /locus_tag="OO145_004060"
                     /gene_synonym="panK"
     CDS             1505..2455
                     /gene="coaA"
                     /locus_tag="OO145_004060"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5468973.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2484..3449)
                     /gene="birA"
                     /locus_tag="OO145_004061"
     CDS             complement(2484..3449)
                     /gene="birA"
                     /locus_tag="OO145_004061"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5468974.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3446..4474)
                     /gene="murB"
                     /locus_tag="OO145_004062"
     CDS             complement(3446..4474)
                     /gene="murB"
                     /locus_tag="OO145_004062"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5468975.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1169 a         1158 c         1041 g         1219 t
ORIGIN      
        1 aggaagccag ccagttccag gattttcgct tcccactctg cgtcgccttc cagcgctttc
       61 agagcagaac cacgaacgat cggagtgtcg tcgcccggga agtcgtactg agacagaagt
      121 tcacgaactt ccatttcaac cagttccagc agctcttcgt catcaaccat gtcgcatttg
      181 ttcaggaaca cgatgatgta cggaacgcct acctgacgac ccagcaggat gtgctcacga
      241 gtctgcggca tcgggccgtc agtcgcagca actaccagga tcgcgccgtc catctgagca
      301 gcaccggtga tcatgttttt aacatagtcg gcgtgccccg ggcagtctac gtgtgcgtag
      361 tgacgggtcg gggtgtcgta ttcaacgtga gaagtgttga tggtgatacc acgagctttt
      421 tcttccggcg cgttatcgat ctggtcgaat gcgcgagcag caccgccgta ggttttagcc
      481 agtacggtag tgattgcagc ggtcagcgtt gttttaccat ggtcaacgtg gccgatagta
      541 ccgacgttaa cgtgcggttt tgtacgttca aacttttctt tagacatcga ttgtccctct
      601 aagacacgga taaatcggtg atatcaccac atcaaccagg caacatgccc gacttgttga
      661 atgcaataaa cagaaggaaa aaacagggag gagaaaagaa gtggtgctga taggcagatt
      721 cgaactgccg acctcaccct taccaagggt gcgctctacc aactgagcta tatcagcaca
      781 tcttggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
      841 ccattatacg atgcccgcat cctagaactc ggctacctga ttttcattct gcactgaata
      901 tcgagagaag ctctctttat tcgagccggt aagtgaactt atcgtctcgg gctacgccat
      961 cgcgtggccg aaattggtgg tgggggaagg attcgaacct tcgaagtctg tgacggcaga
     1021 tttacagtct gctccctttg gccgctcggg aaccccaccg gacttgatgg tgccgactac
     1081 cggaatcgaa ctggtgacct actgattaca agtcagttgc tctacctact gagctaagtc
     1141 ggcatcaagt agcgcgcatt ctatggagac atgcgagttc atgcaactaa aaaattgcat
     1201 aatttgtttt attggtcaca ttttatgcta cacgatgaag aaacagccga acggagggca
     1261 gattagcaca ctttttcaac atcattgtgc tcaacaatgc gctcctgcta aaccataatt
     1321 ctttttatca gatggaatat ctgtcacatt gcttttcaac gatagcttcc tggcagagat
     1381 tttttcttat tattcctccc catctggtgt taccctcctg cccattaacc cattcaacag
     1441 aactgtgacg cgccatggca aatatcgctt tgccgataga gctatgaccg ccagaaacat
     1501 gcttatgagt ataaaagagc aaacgttaat gacgccttac ctacagtttg accgcaacca
     1561 gtgggcagct ctgcgtgatt ccgtacctat gacgttatcg gaagatgaga tcgcccgtct
     1621 caaaggtatt aatgaagatc tctcgttaga agaagttgcc gagatctatt tacctttgtc
     1681 acgtttgctg aacttctata taagctcgaa tctgcgccgt caggcagttc tggaacagtt
     1741 tcttggtacc aacgggcaac gcattcctta cattatcagt attgctggca gtgtcgcggt
     1801 ggggaaaagt acaaccgccc gtgtattgca ggcgctatta agccgttggc cggaacatcg
     1861 tcgtgttgaa ctgatcacta cagatggctt ccttcaccct aatcaggttc tgaaagaacg
     1921 tggtctgatg aagaagaaag gcttcccgga atcgtatgat atgcatcgcc tggtgaagtt
     1981 tgtttccgat ctcaaatccg gcgtgccaaa cgttacagcc cctgtttact cgcatcttat
     2041 ttatgatgtg atcccggatg gagataaaac tgttgttcag cctgatattt taattcttga
     2101 agggttaaat gtcttacaga gcgggatgga ttatccacac gatccacatc atgtatttgt
     2161 ttccgatttt gtcgattttt cgatatatgt tgatgcaccg gaagacttac ttcagacgtg
     2221 gtatatcaac cgttttctga aattccgcga aggggctttt acagacccgg attcctattt
     2281 tcataactac gcgaaattaa ctaaagaaga agcgattaag actgccatga cattgtggaa
     2341 agagatcaac tggctgaact taaagcaaaa tattctacct actcgtgagc gcgccagttt
     2401 aatcctgacg aaaagtgcta atcatgtggt agaagaggtc agactacgca aataatttgc
     2461 aggggagcga atactcccct ttcttatttt tctgcactac gcagggatat ttcaccgccc
     2521 atccagggtt ttattattcc atcctgctca agtaataaag ccccctgttt gtctattccg
     2581 cgtgaaatgc caaatatttc tttatcacca atgataagtt tcactgggcg attaataaaa
     2641 ttatccagct tttcccagcg cgacagataa ggtgccaatc cttcttgttc gaagagttcc
     2701 aacgcagcac gtaattcacg tattagcatg gccgccaacg tattacgatc aagattgatc
     2761 cccgcttcct gcagcgtgat ccacccctga ttaacgacac tctcttcaac acggcgcatt
     2821 gccatgttga tcccggctcc aatgactatt tgcgccgcat cgccagtttt gccagtcagc
     2881 tccacaagaa tgcctgccag cttgcgatcc tgcagataga ggtcattagg ccatttgaca
     2941 cgaactttat ctgctcccag ctttcgtaat acttccgcca tcacgatacc gataaccaga
     3001 cttaaaccaa tcgccgccgc cgggccttgt tccagacgcc agaacatcga caaatataag
     3061 tttgcgccaa aaggcgaaaa ccatttccgc ccccggcgac cacggccagc ctgctggtat
     3121 tctgcaacac aggcatcgcc cgatttaagc tctccgatac gatcaagaag gaactgattc
     3181 gtggagtcaa taactggcag cacggctaca ctaccgccat ccagctgacc caatatctgt
     3241 tcagcattaa gtaactggat gggctcaggc aggctgtatc ctttacccgg aacggtaaag
     3301 acatcaacgc cccagtcacg cagtgtctga atgtgtttat taatagccgc ccggctcatt
     3361 cccagcgttt cacccaactg ctcgccagag tgaaattcac cgttcgctaa cagggcaatc
     3421 aatttcagtg gcacggtgtt atccttcatg aaattgtctc cactgcgctc acttcacctg
     3481 atgcaccaat aaagcggact tcaggctcaa gccagacatt aaatttttca cccacttttt
     3541 gtcttacatg gtgtgccagt tgcaccacat cttcgctttt tgcattgtct tcattaatga
     3601 gaactaacgc ctgttgacgg tgcaccgcag ccccacccat ttgcatccct tttagctggc
     3661 actgatcaat aagccaacct gctgccagtt ttactgaacc acccgcctgg gggtaatttg
     3721 gtgctgttgg aaattgcgcc agtaatgctt tagccgtttc ggcagataca acagggtttt
     3781 tgaagaaact accggcattg ccattcactt ttggatcagg gagtttggtg gtgcgcatat
     3841 gacacaccgc attaaatact tgctgtggcg ttactgttgt aggatccaga cgagttaagt
     3901 caccatacgt tagtacaggt tgccactctt ttggcagacg cagacctacg gctacaatgg
     3961 cgaagcgatc ctggtattca tgtttaaaaa tactgtcgcg atagccaaaa cggcactctt
     4021 tggcagttaa gcgcacttgc ttgcctgtcg ccagttcaac acaatcaaca taagcgcaaa
     4081 ctcgctgtaa ttctacgcca taagcaccaa tattctggat aggtgatgag ccgacacaac
     4141 caggaattaa tgccagattt tccagaccag gcataccttc ctgcaaagtg tattttacca
     4201 ggcgatgcca gttttctccg gctcctacat gtaaatacca cgcatcaggt tcatcatgaa
     4261 tttcgatacc tttgatccgg ttgatgatca ccgtgccgcg atagtcttcc agaaaaagta
     4321 cattacttcc ttcacccaga ataagaacgg gttgtccttc tgcggttgca tgctgccagg
     4381 cattgagtaa ttgttgttcg tcttcggcac atacaatgtg ctgagcatta tgatcaatgc
     4441 caaatgtgtt ccagggtttt aaggagtggt tcatagctgc tttcctgatg caaaaacgag
     4501 gctagtttac cgtatctgtg gggggatggc ttgtagatat gacgacagga agagtttgta
     4561 gaaacgcaaa aaggcccgac tgggctt
//
LOCUS       DAJTMX010000218         4550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTMX010000218 DAJTMX010000000
VERSION     DAJTMX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.218"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1224
                     /locus_tag="OO145_004063"
     CDS             <1..1224
                     /locus_tag="OO145_004063"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5468976.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="OO145_004064"
     CDS             1224..1958
                     /locus_tag="OO145_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5468977.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2426..3187
                     /locus_tag="OO145_004065"
     CDS             2426..3187
                     /locus_tag="OO145_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5468978.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="OO145_004066"
     CDS             3231..4310
                     /locus_tag="OO145_004066"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5468979.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="OO145_004067"
     CDS             4307..>4550
                     /locus_tag="OO145_004067"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5468980.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          864 c         1120 g         1319 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAJTMX010000219         4291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTMX010000219 DAJTMX010000000
VERSION     DAJTMX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.219"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..1224
                     /locus_tag="OO145_004068"
     CDS             <1..1224
                     /locus_tag="OO145_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5468981.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO145_004069"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO145_004069"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5468982.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO145_004070"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO145_004070"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5468983.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO145_004071"
     CDS             2714..3427
                     /locus_tag="OO145_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5468984.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO145_004072"
     CDS             complement(3399..4076)
                     /locus_tag="OO145_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5468985.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..>4291)
                     /locus_tag="OO145_004073"
     CDS             complement(4070..>4291)
                     /locus_tag="OO145_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5468986.1"
                     /translation="IACAIAMQPKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLI
                     ILFERHRTPFLNYCQQAWQLQNGALQPLC"
BASE COUNT         1006 a         1014 c         1253 g         1018 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga t
//
LOCUS       DAJTMX010000220         4211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTMX010000220 DAJTMX010000000
VERSION     DAJTMX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.220"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..437
                     /gene="tnpA"
                     /locus_tag="OO145_004074"
                     /pseudo
     CDS             1..437
                     /gene="tnpA"
                     /locus_tag="OO145_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(555..1499)
                     /gene="hypT"
                     /locus_tag="OO145_004075"
                     /gene_synonym="qseD"
     CDS             complement(555..1499)
                     /gene="hypT"
                     /locus_tag="OO145_004075"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5468987.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1844..2563
                     /locus_tag="OO145_004076"
     CDS             1844..2563
                     /locus_tag="OO145_004076"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5468988.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2629..3927
                     /locus_tag="OO145_004077"
     CDS             2629..3927
                     /locus_tag="OO145_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5468989.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          912 c         1036 g         1203 t
ORIGIN      
        1 gcacacccga tggaactgta aatatcacat agttttttgc gccaaaatac cgaagacagg
       61 tgttctacag agagaagcgt agagcaacag gcagtatttt gagaaagctg tgtgagtgga
      121 aaagcgtacg gattctggaa gctgaatgct gtgcagatca tatccatatg cttgtggaga
      181 tcccgcccaa aatgagcgta tccggcttta tgggatatct gaaagggaaa agcagtctga
      241 tgctttacga gcagtttggt gatttgaaat tcaaatacag gaacagggag ttctggtgca
      301 ggggggacta cgtcgataca gtgggtaaga acacggcgaa gatacaggat tacataaagc
      361 accagcttga agaggataaa atgggagagc agttatcgat tccctgtccg ggcagcccgt
      421 ttacggaccg taagtaacga agttggatgc aaatgtcaga tcgtgtgcgc ctgttagggc
      481 gcggctggta agagagcctt ataggcgcat ttgaaaaacc tccggctatg ccggaggata
      541 tttattgcac gtcgtcaatt gcggcgtgct ggctcttcca ttgatatctg ctggattaaa
      601 tcgtctggca tatgttcctg taaaatacgc cagaggttct cggcggttct attcagtcgg
      661 gtgttcattc ggtaaatata agcctgaatg gggactgtca atttagcagc atcaaggcat
      721 atcagacgtt tggtctgtag ttcatcgacg atcgaccaga tcggtaacca ggcaatgccg
      781 tatccgttga gcgccatatt ctttaataac tcgctcatcg aagagataaa tatcgtgcgc
      841 ccggtaatac ctccaacctc tgccagatga cgattgacca acctgcccat ataagatgtg
      901 gcggtatagt tgagcaaagg aacctggggc tgagtgatat cgaatacagg gtgcccctgt
      961 gcgtcggcgg cacataccgg gtacaactca gattcaaaca gtttcaggca gcaaaaaggc
     1021 gattgcataa gatcttcatc gcgaaacgaa ataataaagt cactcttgcc ttcgcgtaac
     1081 gtatttacgg cctggacaac atcaatggct tcaacatgat agacaaattc tccgccatag
     1141 gcggtaagtg aatgcaccag tttgggtaat acgctcaggg atagtgagtg cgcggctgca
     1201 attttaatgt tgggaacgcc gagtaaactc tggccgttta actcccccaa attgcattca
     1261 agctgctgta ataagctgcg agtctgggaa tgaaagagct tgccctcttc ggttaattgt
     1321 aaaggggtgg tcgtgcgatc gaataaaaca acgccgatcg ctgcctccag cgccttaatt
     1381 cgtcggctga atgcgggttg cgaaagatta cgctcttttg ccgcctgaga aaaatggcga
     1441 caggcttcca gtgtcaggaa atcgtataac cattttgtct cgatattctt cattaccatg
     1501 gcacctgtat tttcaatcca aagacataat aaaacatccc ggcaataaaa ataatgttct
     1561 caattagcac aacctggcct ttatcattcg ttttgtgcat aaccccttca tttccggtat
     1621 tgacgttgat atcacatttc gccaaaaaaa cgaaattttt ccgtcagata accagaatac
     1681 atcatcacta tggtgaataa gcggaatgtt ccatagctgc atcatgcgtg tcttgcatcg
     1741 ccttgtacaa ccttgcattt ctcatcgccc atttcgactg ttaaaaaaga gagcagcagt
     1801 tatttaccgc gtattgtcat taacgttttg agaggcaggt attgtgaaag gtttaattgg
     1861 tgtactcggt ggaatggggc cggcagctac cgtcgacctg tttaataaat ttgtaacttt
     1921 taccgctgca caatgtgatc aggaacatat tcctttaatt atttcttcga tcccggatat
     1981 tcctgaccgt accgatgcat taatgcatca tgggcgttca ccattacccg ttatgcgtga
     2041 ttatatgcat aagctggaag atgccggggc agaatgtatt gttattccct gtaataccgc
     2101 gcattactgg ttcaaagaat taaaagatgc ttgtcatatt gatatattaa gtatcgtaga
     2161 aactacgatc aatgaagttc gcgcctgtgg taaaacccga attggcttgt tagccaccaa
     2221 tgccacgcta tatatgggtt tataccaaaa aggaattgag tcgctgggat ttacctgtgt
     2281 cagccccgat gccgacggtc agaaaaaagt gatggaaagt atttattcct tgaaagctgg
     2341 caatatagcg cacgcacaaa agttgatgaa tgagcaggct gaaatattat tttcccgcgg
     2401 tgcagaaatc cttgttcttg gttgtacaga aattccggtt attctggcgc aagccgttaa
     2461 agagcagcct tcccgctata ttgactcaac ggcgtcactc gttcgtgccg gaataaaatg
     2521 gtacgaaaat cgtgtcggta aacattatct tttaacccaa taatcattta aatcgcagcc
     2581 agaaagttat tcgcttttaa ctgaattata tttataacgg agaacattat ggtttggctg
     2641 gaaattatcg tagtacttgg tgcaatattt tttggtattc gccagggggg aatcggtatt
     2701 ggtttatgtg gcgggcttgg gcttgccatt ctgactctgg gatttggtct gcctatgggg
     2761 tcgccgccag tcgacgtgat cctgattatt atgaccgttg ttgtggcggc ctcagcctta
     2821 caggccgcag gcggaatgga ttatctggta cgcgtcgctg gtaattttat gcggcgtaac
     2881 ccgaaatata tcaacataat cgcgccgatt atcacctggt ctatgaccat tatggccggt
     2941 actgggttta ttgttttttc cacgctgccg gtgattgctg aagtggcgaa agagtccggt
     3001 atccgtcctt cccgcacgct tgccggttcg gttgtcgctt cgcaggttgc gatttccggt
     3061 tcgccgatca gcgccgcaat ggcggctatg ctcactatta tggaggtaaa cggtgtcagt
     3121 tttattcaag tgatgtcggt atgtcttccc acctcttttg ctgccgcgat ggtcgccgct
     3181 tttattgctt cccgacaggg atgtgagttg caggatgacg aggtctacct tgagcgcctg
     3241 caaaaaggac tggtgcaaaa atacgagaat aatagcagca tcaagccagg tgcgacgctc
     3301 tccgtcggat tgttcctgct ggcgacggtc gctattgtta ttcttgctgc cttcccccaa
     3361 ctgcgtccgg gttttgatat cggtaaaccg atggaaacac gcgatatcat tattatctgt
     3421 atgctctctg ccgcctgcct gatggtggtg ctgtgtaaga cggcgaccga tgccattatt
     3481 ctcacctcca cattccgtgc ggggatgagt tcgctggcgg taattctcgg cattgtaacc
     3541 ttaggcacca cttttattga tgcgcattta actgagatta aagatattgc tggtgatatt
     3601 ttgcaggcgt atccgatgtt gttggcgctg gttctttttg gcacttgcgc gctgctctac
     3661 tcgcaaggtg cgaccacgcc gttaattatc ccgcttgccg tcgcgcttga cgtgcccacc
     3721 tgggcaattc tggcgtccta tgtggccgtc accggagtat ttgtactgcc aacgtatcca
     3781 acatcactgg ctgccatgga atttgatact accggcacaa ctcgtgtagg caaatatgtg
     3841 ctgaatcatc catttatgct gcctgggttg gggggaatta ttgctggagt ggcattaggc
     3901 tttgtgattg cgccaatgat tatttagcac tatcaaacat cagtatcgat ggtttataca
     3961 caataaagac agatatggcg gaatatagtc tgttgtaagc gccaacaaag aataggggaa
     4021 ttccgccatg tctgaacatc ttcacaatat ggtattaagt gttgtcgttt aatgatttat
     4081 ttcttggtta tgttgtgtat tagctgcatc tatcgctttt tgatacctga ggtgtactgg
     4141 caatagcgga cactaccatt tgttcttttt ttaagcagtt atctgatgat atttttccct
     4201 gaaggctgcc g
//
LOCUS       DAJTMX010000221         4198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTMX010000221 DAJTMX010000000
VERSION     DAJTMX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.221"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            84..752
                     /locus_tag="OO145_004078"
     CDS             84..752
                     /locus_tag="OO145_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5468990.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO145_004079"
     CDS             complement(995..1690)
                     /locus_tag="OO145_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5468991.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO145_004080"
     CDS             complement(1683..3110)
                     /locus_tag="OO145_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5468992.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO145_004081"
     CDS             complement(3121..3840)
                     /locus_tag="OO145_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5468993.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAJTMX010000222         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTMX010000222 DAJTMX010000000
VERSION     DAJTMX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.222"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO145_004082"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO145_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5468994.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="OO145_004083"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="OO145_004083"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5468995.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          960 g         1034 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTMX010000223         4172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTMX010000223 DAJTMX010000000
VERSION     DAJTMX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.223"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..176)
                     /locus_tag="OO145_004084"
     CDS             complement(<1..176)
                     /locus_tag="OO145_004084"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5468996.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAE"
     gene            complement(317..2350)
                     /gene="metG"
                     /locus_tag="OO145_004085"
     CDS             complement(317..2350)
                     /gene="metG"
                     /locus_tag="OO145_004085"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5468997.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2482..3591
                     /gene="apbC"
                     /locus_tag="OO145_004086"
     CDS             2482..3591
                     /gene="apbC"
                     /locus_tag="OO145_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5468998.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3853..4134
                     /locus_tag="OO145_004087"
     CDS             3853..4134
                     /locus_tag="OO145_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5468999.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1015 a         1039 c         1062 g         1056 t
ORIGIN      
        1 ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga aaaacttttt
       61 atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc gtttccctgt
      121 tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg tctcattctg
      181 catccctata attttatcca gttgaatgca gatgctacca gtatttatac gggttagaga
      241 gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc tgtcgatgca
      301 gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca tccgggctta
      361 gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca gagataccga
      421 agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca atcagtgcct
      481 gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca ccgccgagat
      541 ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt tcgatcagcg
      601 ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga atcggatcat
      661 ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta gaggcttcca
      721 ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac ggattcactt
      781 tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg aggaaggctt
      841 ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc acgcggaaca
      901 ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc tgttttgcga
      961 ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt gccatgattt
     1021 cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact tcagcggcat
     1081 cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca ccgtcaaaac
     1141 gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc acgatatcgg
     1201 cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg cgcgaagaga
     1261 gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc caggtgctgg
     1321 ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc acatagccat
     1381 gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca ggccagaaca
     1441 ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg gtggagtctt
     1501 tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga ttcttgaaag
     1561 aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc ggtgcgttcg
     1621 gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc aggccggatt
     1681 caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg cgggtccatg
     1741 cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa tcacgcatca
     1801 ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg ctgtaggtcg
     1861 cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc gggcaggtgc
     1921 ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc tgagagatgg
     1981 tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct gacaactggc
     2041 ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag cctgcgaaat
     2101 cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata ccgagctgct
     2161 gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag ttgacctcgt
     2221 ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc agcatatggc
     2281 cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt ttcttcgcga
     2341 cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag taccaaatgg
     2401 gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct gatggtttat
     2461 ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc agaagccttg
     2521 cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa gcataacctc
     2581 accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca tgtggaactg
     2641 gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg tagcgccgaa
     2701 ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa catagccact
     2761 ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat cgcggtgagt
     2821 tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct ggcactggcg
     2881 gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc aattcccacc
     2941 atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat ggcaccgatt
     3001 atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga caatgcgatg
     3061 gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga aaccttgtgg
     3121 ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat tcaattgacg
     3181 ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca ggacatcgcg
     3241 ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc ggtgctcggt
     3301 attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga accgattttc
     3361 ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct ggggcagatg
     3421 ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt tatcagccgt
     3481 ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc agctcagctc
     3541 tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta acgcctatta
     3601 accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt gtatttgttt
     3661 ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct tcgtccgaaa
     3721 ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg tgctgtgtag
     3781 tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc tttactacag
     3841 ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc ctatggattg
     3901 gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag gatcagtccc
     3961 ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg tcagttaacg
     4021 tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac gacagaagtg
     4081 gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga ttaatttggc
     4141 aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTMX010000224         4168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTMX010000224 DAJTMX010000000
VERSION     DAJTMX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.224"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO145_004088"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO145_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5469000.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO145_004089"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO145_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO145_004090"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO145_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="OO145_004091"
     CDS             3787..>4168
                     /locus_tag="OO145_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5469001.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAJTMX010000225         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTMX010000225 DAJTMX010000000
VERSION     DAJTMX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.225"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..158)
                     /locus_tag="OO145_004092"
     CDS             complement(<1..158)
                     /locus_tag="OO145_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5469002.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO145_004093"
     CDS             complement(308..469)
                     /locus_tag="OO145_004093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469003.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO145_004094"
     CDS             complement(466..654)
                     /locus_tag="OO145_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5469004.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO145_004095"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO145_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5469005.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO145_004096"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO145_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO145_004097"
     tRNA            complement(2161..2237)
                     /locus_tag="OO145_004097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO145_004098"
     tRNA            complement(2339..2414)
                     /locus_tag="OO145_004098"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO145_004099"
     CDS             complement(2559..3380)
                     /locus_tag="OO145_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5469006.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO145_004100"
     CDS             complement(3395..3751)
                     /locus_tag="OO145_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5469007.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="OO145_004101"
     CDS             complement(3764..>4114)
                     /locus_tag="OO145_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5469008.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTMX010000226         3980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTMX010000226 DAJTMX010000000
VERSION     DAJTMX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.226"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..415
                     /gene="torD"
                     /locus_tag="OO145_004102"
     CDS             <1..415
                     /gene="torD"
                     /locus_tag="OO145_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5469009.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(508..813)
                     /gene="cbpM"
                     /locus_tag="OO145_004103"
     CDS             complement(508..813)
                     /gene="cbpM"
                     /locus_tag="OO145_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5469010.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(813..1733)
                     /gene="cbpA"
                     /locus_tag="OO145_004104"
     CDS             complement(813..1733)
                     /gene="cbpA"
                     /locus_tag="OO145_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5469011.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1994..3250
                     /locus_tag="OO145_004105"
     CDS             1994..3250
                     /locus_tag="OO145_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5469012.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3543..>3980
                     /locus_tag="OO145_004106"
     CDS             3543..>3980
                     /locus_tag="OO145_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HCR5469013.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMD"
BASE COUNT         1096 a          850 c          942 g         1092 t
ORIGIN      
        1 cacgctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt
       61 tctgatgacc gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac aggacgagca
      121 agagattaaa cgcttgttag ttgaggcagg gatggaaacc agtggcaatt tcaacgaacc
      181 ggcagatcat ctggcgatct atctcgagtt gttgagttat ctgcattttt cgctgggaga
      241 ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga cggcgctgcg
      301 gcaatggtta ccagagtttg ctgcgcgttg tcgacagtat gacagctttg gtttttacgc
      361 ggcactaagc cagttattgc tggtgttagt ggagtgcgac caccaaaaca gataacgtcg
      421 tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc attatggcgc agttgccgga
      481 tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg aaagccgctg
      541 gcgtagcagg cggttttcct gcttcaggtg cgcaatatca tccattaacg tcagcgccac
      601 cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc gttgcaccac
      661 aatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg gttcaaccac
      721 ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc
      781 ggtaatagta aaagtcaccg taacattagc cattatgctt tcccccaatc tttacgtgga
      841 tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt
      901 ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc
      961 agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg gatagtcagc
     1021 aaaatgcttt ctttcagtgt tggaacggtg actttagtac ccagcgccgc ttcccacggg
     1081 ctaaccggca ccacaatttc cagatcctgg ccgacaatat cgaacagcgg atgtggcgca
     1141 atatgaatca ccagccacaa atcgccattt ggaccgccgt tttcgcccgg cgtcccctgg
     1201 cctttcaggc ggatgcgttg accattgccg acgcctgccg ggatcttcac attcagcgtt
     1261 ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg
     1321 gtacgcttat gctcagtaag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg
     1381 tggccacgtg cggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag
     1441 aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg gaactgacgg
     1501 ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca
     1561 tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc atccggttct
     1621 ttgctgacat caggatggta tttacgggcc agtcgacgat aggcggtctt gattgtcttg
     1681 agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta
     1741 tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg
     1801 gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgcggagagg
     1861 aggtcgccat caagatgggt tgctgaacat attttaaaca ggtgaaaaag ggtgagcgat
     1921 ttttgatagt tgaaccaggc actttaagtt taactagggc gtcattattt attaaatttt
     1981 atagacgcta tatatgggta gtaatataca tggaattagt tgcactgcaa ataattattt
     2041 gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa aatcaaacat attcaatcac
     2101 gctttttgaa aacacactgg tgtgttttat gcggttgtac aatgaactca gacgtaaagt
     2161 aaatgaagag gatactccat gtctggaatg tgaatcacta gaaaaagaat ttgaggaaat
     2221 gcagaatgat aatgatctat cattatttat gagaacattg cgtactaatg atacacaaat
     2281 ttattcaggg gtttcaggag gtattacata tactatacaa tatgttcaag atgttgatat
     2341 tgttagagtg tccttgccgg gcagaggttc agagtctatc acagatttta aaggttatta
     2401 ttggtatggc tttatggaat atattgaaaa cattaatgcg tgtgatgatg ttttttctga
     2461 gtattgttta gataatgaaa atatgagtat ccagccagaa cagataaata tgccgggaat
     2521 atctgattta gatactggta ttgatttgtc tggcatatct tttattcaga gtgaaattaa
     2581 taaaacatat ggattaaaat acgctccagt agatggcgat ggatattgtc tgttaagagc
     2641 catactggtt ttaaaagaac atgagtattc atgggcgctg ggcagccata agacgcaaaa
     2701 gcaagtatac gaagagttta ttaaaattgt tgataaacaa acgatcgagg ctcttgttga
     2761 tacggcattc tataatctca gggaagatgt aaagacatta tttggcgttg atctgcaatc
     2821 tgacaacaaa attcagggtg tgggtagttt tatgtcatgg agctttctgt tttttaaaaa
     2881 acaattcatt gatagttgct tgaatgacaa aaaatgcata ctgcatttac ccgagtttat
     2941 atttaatgat aacaagaatt tgcttgcttt agataccgac acgtcggata ggattaaagc
     3001 ggtgaagaat tttcttgctg ttctttcaga tagcatttgc tcattattta ttgttaatag
     3061 taatgtggca tcaatctcct tgggaaatga atccttttca acagatgaag atcttgagta
     3121 tggatattta acaaacactg gcaatcatta tgatgtttac ctccctcctg aacttttttc
     3181 tcaggcttat aagttaaaaa ataaggaaat gaatgcgcaa cttgactatt taaatcgtta
     3241 tgcaacttaa tggcaaaggc atatgctaaa aaccattgtt attagtctca cactttttta
     3301 ttggtaaata ttgtctctgt attggtaacg ccgcagatat tctgtttagc cacaggtgca
     3361 attatcagcg gcgtacgcga ggcaggggct aatcaggcat agtttgcgtc aaaccttgcc
     3421 tgtttttgaa gatgtatata gaaaaacagg cgttcaacaa gccattttgc gaacctgttc
     3481 ccggaaaaaa gtcatatttc tgtcacactc tttaatgatt gataacaaaa gaggtgccag
     3541 gaatgaacaa aacgctaatc gccgcaactg tggcagggat agttttactc gcttcaaatg
     3601 ctcaggcaca aaccgtaccg gaaggctatc agctacagca agtgctcatg atgagccgcc
     3661 ataacttacg tgcgccgctg gcgaacaatg gcagtgtgct ggagcagtcg acgccgaata
     3721 aatggcccga atgggacgtc cccggtgggc aactcaccac caaaggtggc gtgctcgaag
     3781 tgtatatggg ccattacatg cgtgaatggc tggcagagca ggggatagtg aaatcggggg
     3841 aatgcccgcc gccggacacc gtttatgcct atgccaatag tctgcaacgt accgtcgcta
     3901 ccgcccagtt ctttattact ggtgcattcc ctgggtgtga tattcctgtg catcaccagg
     3961 aaaaaatggg cactatggac
//
LOCUS       DAJTMX010000227         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTMX010000227 DAJTMX010000000
VERSION     DAJTMX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.227"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            437..2089
                     /locus_tag="OO145_004107"
     CDS             437..2089
                     /locus_tag="OO145_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5469014.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO145_004108"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO145_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO145_004109"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO145_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO145_004110"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO145_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTMX010000228         3690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTMX010000228 DAJTMX010000000
VERSION     DAJTMX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.228"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..224
                     /locus_tag="OO145_004111"
     CDS             <1..224
                     /locus_tag="OO145_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5469015.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO145_004112"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO145_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5469016.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO145_004113"
     CDS             518..1108
                     /locus_tag="OO145_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5469017.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO145_004114"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO145_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5469018.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO145_004115"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO145_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5469019.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO145_004116"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO145_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5469020.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO145_004117"
     CDS             3457..3648
                     /locus_tag="OO145_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469021.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          998 a          902 c          929 g          861 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgct tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga
//
LOCUS       DAJTMX010000229         3602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTMX010000229 DAJTMX010000000
VERSION     DAJTMX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.229"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..3580
                     /gene="entF"
                     /locus_tag="OO145_004118"
     CDS             <1..3580
                     /gene="entF"
                     /locus_tag="OO145_004118"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5469022.1"
                     /translation="NIDPHGTAQALMLADLQQDLRVDSGKPLVFHQLIQVADNRWYWY
                     QRYHHLLVDGFSFPAITRQIANIYCALLRGEPTPASPFTPFADVVEEYQQYRESEAWQ
                     RDAAFWVEQRRQLPPPASLSPAPLAGRSASADILRLKMEFTDGEFRQLATQLSGVQRT
                     DLALALTALWLGRLCNRMDYAAGFIFMRRLGSAALTATGPVLNVLPLGIHIAAQETLP
                     ELATRLATRLAAQLKKMRRHQRYDAEQIVRDSGRAAGDEPLFGPVLNIKVFDYQLDIP
                     GVQAQTHTLATGPVNDLELALFPDEHGDLSIEILANKQRYDEPTLIQHAERLKMLIAQ
                     FAADPALLCGDVDIMLPGEYAQLAQINATQVEIPETTLSALVAEQAAKTPDAPALADA
                     RYLFSYREMHEQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPL
                     DTGYPDDRLKMMLEDARPSLLITTDDQLPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQ
                     PHHTAYIIFTSGSTGRPKGVMVGQTAIVNRLLWIQNHYPLTGEDVVAQKTPCSFDVSV
                     WEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASLTPQTA
                     RQSCVTLKQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAFGEELAQ
                     VRGSSVPIGYPVWNTGLRILDAMMHPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFI
                     ADPFAPGERMYRTGDVARWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRVMQALPDVK
                     QAVTHACVINQAAATGGDARQLVGYLVSQSGLPLDTSALQAQLRETLPPHMVPVVLLQ
                     LPQLPLSANGKLDRKALPLPELKAQAPGRAPKAGSETIIAAAFASLLGCDVQDADADF
                     FALGGHSLLAMKLAAQLSRQFARQVTPGQVMVASTVAKLATIIDGEEDSSRRMGFETI
                     LPLREGNGPTLFCFHPASGFAWQFSVLSRYLDPQWSIIGIQSPRPHGPMQTATNLDEV
                     CEAHLATLLEQQPHGIAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAE
                     INREREAFLAAQQGSTSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQE
                     GMSPERAWSPWIAELDIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT          751 a          970 c         1061 g          820 t
ORIGIN      
        1 caatattgat ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt
       61 gcgcgtcgat agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg
      121 ctggtactgg tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat
      181 tacccgccag atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc
      241 gccgtttacg cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc
      301 ctggcagcgt gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc
      361 actttctccg gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat
      421 ggaatttacc gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac
      481 cgatttagcc cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta
      541 cgccgccgga tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc
      601 cgtgctcaac gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact
      661 ggcaacccga ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg
      721 ttacgatgcc gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt
      781 tggtccggta ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc
      841 gcaaacccat accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga
      901 tgaacacggt gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac
      961 gttaatccag catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc
     1021 gctgttgtgc ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat
     1081 caacgccact caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc
     1141 ggcaaaaaca ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga
     1201 aatgcacgag caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg
     1261 ggacagcgtg gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat
     1321 agttgaagct ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa
     1381 aatgatgctg gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg
     1441 ctttagcgat gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg
     1501 cagtgcgccg ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg
     1561 ctccaccggc aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt
     1621 gtggattcaa aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg
     1681 cagttttgat gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt
     1741 gatggctgaa ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata
     1801 tggcgtaacg accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac
     1861 tccgcaaacc gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc
     1921 cttaccggct gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct
     1981 gtatggcccg acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact
     2041 ggcacaggtg cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg
     2101 tattcttgat gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac
     2161 cggtattcaa ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat
     2221 tgccgatcct tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct
     2281 ggataacggc gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca
     2341 gcgtatcgaa ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc
     2401 cgttacccac gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt
     2461 ggtgggctat ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca
     2521 gcttcgcgaa acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt
     2581 gccacttagc gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc
     2641 acaagcgcca gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc
     2701 gtcgttgctg ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca
     2761 ttcgctactg gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac
     2821 gccggggcag gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga
     2881 agaggacagc tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg
     2941 cccgacgctg ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc
     3001 gcgttatctc gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc
     3061 catgcagaca gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga
     3121 gcaacaaccg cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct
     3181 tggtttgctg gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg
     3241 tctggacccg gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca
     3301 gcagggaagt acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt
     3361 gcgcctgctg acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc
     3421 tgaacgcacg cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga
     3481 gctggatatc tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt
     3541 aaaaattggg ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc
     3601 ca
//
LOCUS       DAJTMX010000230         3548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTMX010000230 DAJTMX010000000
VERSION     DAJTMX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.230"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO145_004119"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO145_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5469023.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO145_004120"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO145_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5469024.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO145_004121"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO145_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5469025.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAJTMX010000231         3456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTMX010000231 DAJTMX010000000
VERSION     DAJTMX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.231"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(226..429)
                     /locus_tag="OO145_004122"
     CDS             complement(226..429)
                     /locus_tag="OO145_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469026.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OO145_004123"
     CDS             complement(431..586)
                     /locus_tag="OO145_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5469027.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OO145_004124"
     CDS             complement(789..1196)
                     /locus_tag="OO145_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5469028.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OO145_004125"
     CDS             1273..1500
                     /locus_tag="OO145_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5469029.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OO145_004126"
     CDS             1484..1906
                     /locus_tag="OO145_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469030.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="OO145_004127"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="OO145_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="OO145_004128"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="OO145_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          765 c          940 g          866 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggcgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAJTMX010000232         3445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTMX010000232 DAJTMX010000000
VERSION     DAJTMX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.232"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..557
                     /locus_tag="OO145_004129"
     CDS             <1..557
                     /locus_tag="OO145_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5469031.1"
                     /translation="FTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALI
                     LLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELILARQRFAHGVQTLF
                     FDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVT
                     DENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(591..1004)
                     /locus_tag="OO145_004130"
     CDS             complement(591..1004)
                     /locus_tag="OO145_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5469032.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            1191..2396
                     /gene="yedE"
                     /locus_tag="OO145_004131"
     CDS             1191..2396
                     /gene="yedE"
                     /locus_tag="OO145_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5469033.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            2393..2626
                     /gene="yedF"
                     /locus_tag="OO145_004132"
     CDS             2393..2626
                     /gene="yedF"
                     /locus_tag="OO145_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5469034.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            2733..3403
                     /locus_tag="OO145_004133"
                     /pseudo
     CDS             2733..3403
                     /locus_tag="OO145_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT          748 a          899 c          922 g          876 t
ORIGIN      
        1 tcttcactgg gacgctggtg gaagccgatc cttttcacgc cgtaaccctc gttgccaacc
       61 acgacaccca accgttgcaa gccctcgaag caccggtcga accgtggttt aaaccgctgg
      121 cgtatgcctt aattttgttg cgggaaaatg gcgttccttc ggtgttctat ccggacctct
      181 acggtgcaca ttacgaagat gtcggtggtg acgggcaaac ctatccgata gatatgccaa
      241 taatcgaaca gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga
      301 cattattttt cgaccatccg aactgcattg cctttagccg cagtggcacc gacgaatatc
      361 ccggctgcgt ggtggtcatg tcgaacggag atgatggcga aaaaaccatt catctgggag
      421 agaattacgg caataaaacc tggcgtgatt ttctggggaa ccggcaagag agcgtagtga
      481 cagacgaaaa cggcgaagcg accttctttt gcaacggcgg cagcgtcagc gtgtgggtta
      541 tcgaagaggt gatttaaatc catccccggc ggcaagccgg ggagatttca ttacggcagt
      601 ggcgtcggta acggcgcttt atccagcgcg gcggcacact ctgccgtcgg gcgatccaca
      661 cgctccatcg tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc
      721 tcacgtttta cggtaacgtt agtgagatca tcagacatca atgtcaactt acccggcacg
      781 gcaatccccc gctgccactg acggcaatcc aaggtatcgc cctctttagt gacaatcagg
      841 ctgcctatcg cttccggact gaccagcgca cgttgcggcc ctttggtctg ccagtagccc
      901 gccagccagt ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag
      961 cccgccagca acatgagcgc gcctgcaatt gcgagttttt tcatcattcg tcctgcatga
     1021 gaagaaaaga ggattgtggc attaaagctc tgatgtcgcc agcctttcca ggttcagaga
     1081 gaaatttgat ctaactatta tttttgtgaa ttattacccc gaaaataaat ttgcatggtg
     1141 tatgattcgc ggctcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc
     1201 agcaattcaa acacgcctgg ttgattaaat tctgggcacc catccctgcg gtcatcgcgg
     1261 cgggtattct ctccacttac tattttggca ttactggcac cttttgggct gtcacgggtg
     1321 aatttacccg ttggggcggt cagctcctgc agctgtttgg cgtccatgct gaagagtggg
     1381 gttattttaa aattatccat ctggaaggat cgccattaac ccggatcgac ggcatgatga
     1441 tcctcggtat gtttggcggc tgctttgctg ctgcgctgtg ggccaacaat gtcaaactac
     1501 gcatgccacg cagccgtatc cgcattatgc aggccatcat tggcggcatt atcgccggtt
     1561 ttggcgcgcg tctggcaatg ggctgtaacc tggcggcgtt ctttaccggc attcctcagt
     1621 tctcgctgca tgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcgc
     1681 gctttaccct gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct
     1741 caccgctgac gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct
     1801 ttttcggcat gctgggctgg gcgctgctca cggcgatgaa ccaaccaaaa ctggggctgg
     1861 caatgctgtt tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc tgctttactt
     1921 cggcgttccg cgatatgtgg atcaccggac gtacccatat ggcgaaagca atcattatcg
     1981 gtatggcggt gagtgccatc gggatcttca gttacgtaca gttaggcgtt gaacctaaaa
     2041 tcatgtgggc gggaccaaat gcggtaattg gtggtttact ttttggtttt ggcatcgtgc
     2101 tggcaggcgg ctgcgaaacc ggctggatgt accgcgcggt agaaggccag gtgcactact
     2161 ggtgggtcgg tctgggcaac gtgatcggct caacgattct ggcgtattac tgggatgatt
     2221 tcgctccggc gctggccacc gactgggaca aaatcaacct gctgaaaact tttggtccga
     2281 tgggaggcct gctggtgaca tatttgctgc tgtttgctgc gctgatgttg attatcggct
     2341 gggaaaaacg cttcttccgc cgtgaggcac cgcagactgc taaggagatc gcatgaaaaa
     2401 tatcgttcct gattaccgcc ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc
     2461 aacccttgag gcgatgccgc aattaaaaaa aggtgagatc ctggaagtgg tgagcgactg
     2521 tccgcagtcg atcaataata ttccactgga tgcgcgtaat cacggctaca cggtgctgga
     2581 tattcagcaa gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc
     2641 tgtggcccac agcgggaaca tcgttacctc tgacacatag attttacccg ccccctctcc
     2701 tattcttttc agtatgatct caggaggttg ctatgtgtgg acgctttgcc caatcccaaa
     2761 cgcgtgagga ttaccttgcg cttctcgcgg aagatattga acgcgatatt ccctacgatc
     2821 ccgaacccat tggcagatac aatgtcgcgc cgggaaccaa agttcttctg ctcagtgaac
     2881 gtgatgaaca ccttcatctg gatccggttt tctggggata tgctcccgga tggtgggata
     2941 aaccgccgct gattaacgcc cgcgtagaaa ctgcctcaac cagtcgcatg tttaaaccgc
     3001 tctggcaaca tggtcgggca atctgttttg ccgatggctg gtttgagtgg aaaaaagaag
     3061 gcgacaaaaa aagccttatt ttatctatcg cgctgacgga caacctgttt ttatggccgc
     3121 gataggtagc ataccgtttg agcgtggtga cgaagccgaa ggatttttga tcgtcactgc
     3181 ggcggcagat caaggtctgg tagatattca tgaccgccgc ccgctggttc tgtcgccaga
     3241 agctgcacgg gaatggatgc ggcaagacat tggtggtaaa gaagcctcag aaatcgctac
     3301 cagaagctgt gtgccagcaa accagttcac ctggcaccct gtgtcgcgca cggtgggtaa
     3361 tgtcaaaaac cagggcgcgg agttaattca acctgtttgt tgacatcctc cacgccctga
     3421 atgacgagga cccctgctac gttca
//
LOCUS       DAJTMX010000233         3414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTMX010000233 DAJTMX010000000
VERSION     DAJTMX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.233"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..238
                     /locus_tag="OO145_004134"
     CDS             <1..238
                     /locus_tag="OO145_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5469035.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO145_004135"
     CDS             248..886
                     /locus_tag="OO145_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5469036.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO145_004136"
     CDS             889..2052
                     /locus_tag="OO145_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5469037.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="OO145_004137"
     CDS             2136..>3414
                     /locus_tag="OO145_004137"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5469038.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAJTMX010000234         3348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTMX010000234 DAJTMX010000000
VERSION     DAJTMX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.234"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            19..897
                     /locus_tag="OO145_004138"
     CDS             19..897
                     /locus_tag="OO145_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5469039.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO145_004139"
     CDS             894..2285
                     /locus_tag="OO145_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5469040.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO145_004140"
     CDS             2282..2539
                     /locus_tag="OO145_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469041.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO145_004141"
     CDS             2634..2834
                     /locus_tag="OO145_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5469042.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO145_004142"
     CDS             2827..3207
                     /locus_tag="OO145_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5469043.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAJTMX010000235         3324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTMX010000235 DAJTMX010000000
VERSION     DAJTMX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.235"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            454..1281
                     /locus_tag="OO145_004143"
     CDS             454..1281
                     /locus_tag="OO145_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5469044.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="OO145_004144"
     CDS             1325..2074
                     /locus_tag="OO145_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5469045.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQTPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="OO145_004145"
     CDS             2071..2640
                     /locus_tag="OO145_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469046.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="OO145_004146"
     CDS             2764..3006
                     /locus_tag="OO145_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5469047.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="OO145_004147"
     CDS             3010..3156
                     /locus_tag="OO145_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469048.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3324
                     /gene="xisR"
                     /locus_tag="OO145_004148"
     CDS             3165..>3324
                     /gene="xisR"
                     /locus_tag="OO145_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5469049.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          928 a          797 c          854 g          745 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaac tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagc
//
LOCUS       DAJTMX010000236         3320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTMX010000236 DAJTMX010000000
VERSION     DAJTMX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.236"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(954..1145)
                     /locus_tag="OO145_004149"
     CDS             complement(954..1145)
                     /locus_tag="OO145_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5469050.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1210..1533
                     /locus_tag="OO145_004150"
     CDS             1210..1533
                     /locus_tag="OO145_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5469051.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1523..2401
                     /locus_tag="OO145_004151"
                     /pseudo
     CDS             1523..2401
                     /locus_tag="OO145_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2737..3129)
                     /locus_tag="OO145_004152"
     CDS             complement(2737..3129)
                     /locus_tag="OO145_004152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469052.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          754 a          787 c          960 g          819 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacagc ttatctgtga gcggatgccg ggagctgcat gcgtcagagg
      961 ttttcaccgt catccccgaa gcgagcgagg caactgcggt aaagctcatc ggcgtggtcg
     1021 taaagcgatt cacagatgtc tgcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     1141 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     1201 cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgc
     1261 tgtgagggta aacagctggc ggtatggatg cggcgggtct gcctgggtga gccggttgcc
     1321 cgttccggga ggctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg
     1381 aataacctga atcaggcagc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg
     1441 gttcaggtgg tggccgcact gatggccatc ggggatgagc tgcgccggct gcgtctggcc
     1501 gtcagggaac aggggacgcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg
     1561 cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct
     1621 tcagggtaat ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata
     1681 cacgtccggt gttctctcgt tcgcggagaa ggagctgccg ccgggagggc gtgaaaaagt
     1741 gatggcgagc tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcattct
     1801 gtgggtggag caccaggaca aggggcggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc cgagaattga
     1921 tgcctggcag acgctggtga atcaccatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgccggacg ctgactctcc ctgataacct gccggaaacg aaacaggcgc ttgctgagag
     2041 cattacacgg tggatagacg ctctttatca tgtcggggag ataaaaggcc gtcaggatgt
     2101 gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcattct atgagcaatc
     2221 ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgag cgacgagttc aggaagctcg aagaatctgt aagcaaggct gtgacatcaa
     2341 acgagacgaa aatcaaagac gctatagccc tgttcacagc ttcgacagag gaatcactga
     2401 aaacacagga acgagttaaa acgcatacgg gcgcgattct ggtgtgtctt ttgtcgctgt
     2461 gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttctgttttc ccgctcaggt
     2521 ccccgtcttc ttcgcttccg aaagcatgaa taaaatccgt ttcagctgtc tctggctggc
     2581 aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt taccgggtgc
     2641 gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc
     2701 gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt gagggaatgg
     2761 tccccatata ctgactttat catagggata gggttggtag tgggtcgatt gggtttcggc
     2821 attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg cgtgagattc
     2881 tcctgggcct ggaaaggcta agtgtttaga attgatggca aaatatcttc cgtccgattt
     2941 ttgaagtagt tgttgtttgg ctgtctccca aagggtgctg ttacaaactt cttgaatttg
     3001 tagtttttgc aggactctta ttactgcgtt gtcatgttcc aattctttag gggagtgtat
     3061 tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa agcacgcaga
     3121 taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg tatgccgtgt
     3181 ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa caaatacggc
     3241 tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt tttgcggcgc
     3301 gtaatctgct gcctgcaaac
//
LOCUS       DAJTMX010000237         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTMX010000237 DAJTMX010000000
VERSION     DAJTMX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.237"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..442
                     /locus_tag="OO145_004153"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO145_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO145_004154"
     CDS             1026..1742
                     /locus_tag="OO145_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469053.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO145_004155"
     CDS             2294..2698
                     /locus_tag="OO145_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469054.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OO145_004156"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OO145_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJTMX010000238         3225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTMX010000238 DAJTMX010000000
VERSION     DAJTMX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.238"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..3087
                     /locus_tag="OO145_004157"
     CDS             <1..3087
                     /locus_tag="OO145_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5469055.1"
                     /translation="TFSINFDDSVDKTAETDEEREARYAALQQEWRQYLYDSERLLKS
                     VAGDWIEKPEQYELAEHGYIVKTAQSGGASSHILSLYDHLLVCNKDVPLFNRFASREV
                     HAAESLLAPGAKFSDRLGHSGDKFPLAKAQRDALSHFLDARHGDILAVNGPPGTGKTT
                     LVLSIIATQWARAALEKSEPPVIIATSTNNQAVTNIIEAFGKDFSQGSGAMAGRWLPE
                     LKSFGAYFPSSSRKAEAAKKYQTEDFFNQVESKEYVEDALLFYLEKAKAAFPGKECSS
                     PEKVIELLHGQLAAKSEQLIRLNATWQTLSQIRAARELIANDIEQYLDNLNKLLSGQE
                     QKVTLLKSAKTEWKKYRAGESLIYSLFSWLPAVRNKRQYQIQLFLEDKLGALIAGNQW
                     SDPETIERNIDGLLNSAEREQTTYRQQIDSAHEIVLKEQQAVQEWQRLAFDLGYEGDE
                     ELSFSQADELADTQIRFPAFLLTTHYWEGRWLMDMASIDDLQDEKKKKGAKGVTARWQ
                     RRMKLTPCVVMTCYMLPGNMQISEHKGQRKFEKSYLYDFADLLIVDEAGQVLPEVAAA
                     SFALAKKALVIGDTEQIPPIWSIAPAIDVGNMLAEKILSGSTQEEITEKYTAIADLGK
                     SAASGSVMKIAQFASRYQYDPELARGMYLYEHRRCYDNIIGYCNTLCYHGKLLPKRGR
                     EESNLMPAMGYLHIDGKGELASSGSRYNLLEAETIAVWLAENQQNIEAHYGKSLHEVV
                     GIVTPFSAQVSTIKQVLGKQDISTGTNEKSLTVGTVHSLQGAERAIVIFSPVYSKHED
                     GGFIDSDNSMLNVAVSRAKDSFLVFGDMDLFEVQPASSPRGLLAKYLFESEKNALSFD
                     YKERKDLKTAGTKIYTLHGVEQHDNFLNQTFENTSKHITIISPWLTWQRLEQTGFLDS
                     MIAACSRGINVTIVTDRSYNTEHNDFEKRKEKQQNFKAALEKLNALGIATKLVNRVHS
                     KIVIGDDGLLCVGSFNWFSATREARYERYDTSMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT          909 a          668 c          837 g          811 t
ORIGIN      
        1 acgttctcta tcaactttga tgacagcgtt gataagactg ccgaaacgga tgaagaacgg
       61 gaagcacgat atgccgcctt gcagcaggag tggcgtcaat atctgtatga ctcagagagg
      121 ctactgaaga gcgttgccgg cgactggatt gaaaaacctg agcaatatga actcgctgag
      181 cacggttata ttgttaaaac ggctcaatct ggcggtgcca gttcccatat cctttctctt
      241 tatgatcacc tgcttgtttg caataaggat gtgccgctct tcaatcgctt cgcctcgcga
      301 gaggttcatg ctgcagagtc tttgctggcc ccaggagcaa aattcagcga caggcttgga
      361 cactccggag ataagtttcc gctggcaaag gctcagcgcg atgccttaag ccattttctg
      421 gatgcaagac atggcgatat ccttgctgtt aatggccctc cgggaaccgg aaaaaccacg
      481 ctggtgcttt ctatcatcgc cacgcagtgg gccagagcgg ctctcgaaaa atctgagcct
      541 ccggttatta tcgcgacttc aacgaataac caggctgtaa cgaacattat tgaggcattc
      601 gggaaagact tttcgcaagg ttcaggtgcg atggccgggc gatggttgcc agagctgaaa
      661 agcttcggtg cttattttcc ctcaagcagt cgtaaagctg aggcagccaa aaaatatcaa
      721 actgaagatt tcttcaacca ggttgagtca aaagagtatg tagaggatgc actgctgttt
      781 tatctggaaa aggctaaggc agcctttcct ggaaaagagt gttcatcccc tgaaaaggtc
      841 attgaactct tgcatggtca gttggcagca aaatctgagc aactgataag actgaacgca
      901 acatggcaaa cgttaagcca gattcgggct gcgcgtgagc ttattgctaa tgatattgag
      961 caatatctcg ataatttaaa taaattactt tccggacaag aacaaaaagt cactctactg
     1021 aagagtgcta aaacggaatg gaaaaaatat cgcgccggtg aatcactgat ctattcatta
     1081 ttttcctggc tcccggcggt tcgcaataag cgacagtacc aaatacagct gtttctcgaa
     1141 gataaattag gcgcgctgat tgcaggaaat cagtggtctg atcctgaaac tatcgaacgt
     1201 aatattgatg ggctgctcaa ttccgctgag cgcgagcaaa caacataccg gcagcagatt
     1261 gactccgccc atgaaatcgt tcttaaagaa cagcaggcgg ttcaggagtg gcagaggctg
     1321 gcatttgatt tagggtatga gggcgacgag gaactgagct tctcacaggc cgatgaactg
     1381 gctgatacgc agattcgctt ccctgcattt ttactgacga ctcactactg ggaaggtcgt
     1441 tggctgatgg atatggccag cattgatgat ctgcaggatg agaagaagaa aaaaggtgct
     1501 aaaggggtaa ccgcccgttg gcaacgtcga atgaaactca cgccatgtgt ggtgatgaca
     1561 tgctatatgc tgcccggtaa tatgcagata agtgagcaca aaggacaacg taaattcgag
     1621 aaaagttatt tgtatgattt tgccgattta ctcattgtcg atgaagccgg gcaggtgctt
     1681 cctgaagtgg ctgctgcctc gtttgcatta gctaagaagg cattagtgat tggcgatacg
     1741 gagcagatcc cgccaatatg gagtattgct cctgcgattg atgtcggtaa catgctggcg
     1801 gaaaaaattc tgtctggcag tacgcaagaa gagattaccg agaaatatac ggcaatcgca
     1861 gaccttggta aaagtgccgc atctggcagc gttatgaaaa tagcgcagtt tgcttcgcgc
     1921 tatcaatatg atcccgaact ggctcgtggt atgtacctat atgaacaccg ccggtgctac
     1981 gacaatatta ttggatactg taatacgctc tgctatcacg gtaagttgtt gcctaaaaga
     2041 gggcgtgaag agagcaattt aatgcccgca atggggtatc tccatattga tggtaaagga
     2101 gagctggcaa gtagtggaag tcgatataat ttgcttgagg ctgaaacgat agcggtctgg
     2161 ttggcagaga accagcaaaa tattgaagcg cattacggta aatcgcttca tgaagttgtc
     2221 ggtattgtga cgccttttag cgctcaggta tccactatca aacaggtgct gggcaaacaa
     2281 gatatcagta caggcacgaa tgaaaagtcg ctcacagtgg gcaccgtgca ctctcttcag
     2341 ggagcggaaa gagcgattgt gatattctcg ccagtctatt caaaacatga agacggcggg
     2401 tttattgata gcgataacag catgctgaat gttgcagtct cccgtgcgaa ggacagtttt
     2461 ctggtcttcg gcgatatgga cctgtttgag gtccagccag cctcatcgcc acggggatta
     2521 ctggcaaaat acctctttga gtcagagaag aatgcgctct cttttgatta taaagagcgt
     2581 aaggatttaa aaaccgccgg gaccaaaatc tacacacttc atggtgtgga gcaacatgat
     2641 aatttcctga atcagacatt tgaaaatacc agtaaacaca tcacgataat ttctccatgg
     2701 ctgacctggc aaaggctgga gcaaaccggt tttcttgatt ccatgattgc ggcgtgttca
     2761 cgtggaatta acgtcacgat agtcactgac agaagctaca acactgaaca taatgatttt
     2821 gagaagcgaa aagagaagca gcagaacttt aaagcggcgc tggagaaact gaatgcgctg
     2881 ggtattgcta caaagctggt aaaccgtgtt catagcaaaa ttgttattgg tgatgatggt
     2941 ttgctgtgtg tgggatcgtt caactggttt agtgcgacac gggaagcgcg atatgaacga
     3001 tacgatacat caatggttta ttgcggtgat aacctgaagg gtgagattga ggctatttat
     3061 aatagtcttg agaggcgtca ggtttagagc aggaatggtg agtagccagc ttaccgatcg
     3121 ttttcgagcg taagatggct gaatggaatg gctattattg cacagtcctt aattataaca
     3181 ttcataccga catgattatc ttctgtccgg aagaatcaga ggctc
//
LOCUS       DAJTMX010000239         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTMX010000239 DAJTMX010000000
VERSION     DAJTMX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.239"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO145_004158"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO145_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5469056.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO145_004159"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO145_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAJTMX010000240         3097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTMX010000240 DAJTMX010000000
VERSION     DAJTMX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.240"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(104..1813)
                     /locus_tag="OO145_004160"
     CDS             complement(104..1813)
                     /locus_tag="OO145_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5469057.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIVRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="OO145_004161"
     CDS             complement(2245..2964)
                     /locus_tag="OO145_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5469058.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          589 c          519 g         1036 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcacaa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAJTMX010000241         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTMX010000241 DAJTMX010000000
VERSION     DAJTMX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.241"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..498
                     /locus_tag="OO145_004162"
     CDS             <1..498
                     /locus_tag="OO145_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5469059.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO145_004163"
     CDS             578..1273
                     /locus_tag="OO145_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5469060.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="OO145_004164"
     CDS             1302..>3069
                     /locus_tag="OO145_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5469061.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAJTMX010000242         3065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTMX010000242 DAJTMX010000000
VERSION     DAJTMX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.242"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..197
                     /locus_tag="OO145_004165"
     CDS             <1..197
                     /locus_tag="OO145_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5469062.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO145_004166"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO145_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5469063.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO145_004167"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO145_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5469064.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO145_004168"
     CDS             1821..2576
                     /locus_tag="OO145_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5469065.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="OO145_004169"
     CDS             2977..>3065
                     /locus_tag="OO145_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5469066.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          797 a          683 c          727 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DAJTMX010000243         3060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTMX010000243 DAJTMX010000000
VERSION     DAJTMX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.243"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO145_004170"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO145_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5469067.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(919..1014)
                     /locus_tag="OO145_004171"
                     /pseudo
     CDS             complement(919..1014)
                     /locus_tag="OO145_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO145_004172"
     CDS             complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO145_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5469068.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO145_004173"
     CDS             complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO145_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5469069.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2111..3060)
                     /locus_tag="OO145_004174"
                     /pseudo
     CDS             complement(2111..3060)
                     /locus_tag="OO145_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          764 a          743 c          736 g          817 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag taggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataataa ggataaacaa
      781 aattggctgt aaaaaaacga tactgtttca gttaaccgtg aatggcctgt ttgcaccgta
      841 ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacggt aagcggctcg
      901 tcagaaccgt attgatattt actgagaggt cagatcaact ttccaaggca acagatcgcg
      961 tacccggttt gccggccagt cctggatatg ctcaatgacg taacgcagcc attttgtcaa
     1021 cgccacgatt tttgacccta gcctgacatt tatattcccc agaacatcag gttaatggcg
     1081 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacgaagatc
     1141 ggcacactgg ccatatcagt ggtcatcatg cgccagcttt catccccgac atgcaccacc
     1201 gggtaaagct cacgggagac tttatctgac agcaggcggg cactggccag ggggatcacc
     1261 atccgtcgtc cgggcgtgtc aataatgtca ctctgtacat ccacaaacag acgataacga
     1321 ctctctcttt tataggcgta aaccttaaac tgcatttcac cagtccctgt tctcatcggc
     1381 aaaagagccg ttcatttcaa taaaacgggc gatctcagcc atcccctgct gattttcagc
     1441 ctgccagcgt tccgcacgca gacggcgggc ctctttttgc atggccgtat ttaccaggcc
     1501 tgagatattc acgtttgcgg atttaagtaa ctgatagctg tcactgtcta tagtgacagt
     1561 aatacgctgc ttcatgataa gccctcaaaa acatacttat gatatgtata tgagtatata
     1621 ttaatggtac aaaccatcaa tatccataaa aagtacacca ttaattcgct ataagttttt
     1681 aatacaattt tttctgctgc gattttttta tactataatt agcatactta taacaccgta
     1741 agtaaattac atgaaagcag gcacagcgtg atccgatttt tctgcgtata acgattttcc
     1801 ggaagtgtaa tacggttgcg ccccgggccg ggcacccttt tctgttgttg tgtacgggcc
     1861 cacagagaat ggtaccggag gcgggctcag aagtactgag tcgggtgatc gcgtcaggcc
     1921 gcgaagtact gacacgggta ttggtgactg gtgatgaagt actgccccgg gtatgatttc
     1981 aggctgattc aggttctgat tctctgtcag tccgcgtggt tgctggcctg gctgatttcc
     2041 tgttgtcagg cgttcaacaa ccgactgcag gttatagccc ggttcacgaa cacctgacgg
     2101 caggtatcac tcacccggtg aaggtaaatc cctcaagagg cagcaactct gccagtcgct
     2161 cctccggcca ttccggcaga cgcgtcagga cgtccgtcag ccaggcatgt ggctccagac
     2221 cattacgttt tgctgtttcc agaaggctca ttatctgtgc cgcccggttt ccggccatca
     2281 gtgaaccggc gaacagccac gctttccgtc ccataacaac cgggcgaatg gcacgctcgc
     2341 agatattgtt gtccaggggc acagctccgt cacccaggaa gcagctcagt tcatcgcgcc
     2401 ggttcagaat atagttaatc gccttattta atggcccgtc gggcggacag cacggctcct
     2461 gctcttcaag ccaggcaaac aggtcattca ctatcggccg ggagtaccgt tatcgccact
     2521 gccgtatttt ttccacaggg cgctcacgga tgagtttctc aatgcggtaa agacccgcga
     2581 tttttctcag ctccacgcct gcacggggct ccctgctggc cttatacaga tttgcgaagc
     2641 cgcgccctgc gtgactccag caacaggctg atgtgatgcc ggggtgatta tccgccagag
     2701 atttgtacac gctgtatccg tcactcacca gcgtactgcc acaccagcac tgcagccagg
     2761 tctcagggta tctgagggcc cgtccggtct gcgagtcaaa acagacgacc ggcggaccgc
     2821 ttctttcacc gctgacgtaa gcacacagcc agccggagca ggatttaccg cctttccggg
     2881 tatcaaggag cctcagggac gtttcatctg catgaataac atcccgtgtc agaagctcat
     2941 ggcgtagcag tttcgccagc ggggccagtg cagctccggc aacaccgacc atatccgcca
     3001 ttgtactgac ggggagctcc acacccattc gggagaaaat gtgctgctgg cgatacaggg
//
LOCUS       DAJTMX010000244         2959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTMX010000244 DAJTMX010000000
VERSION     DAJTMX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.244"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO145_004175"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO145_004175"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5469070.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="OO145_004176"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="OO145_004176"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5469071.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="OO145_004177"
     tRNA            2651..2726
                     /locus_tag="OO145_004177"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>2959)
                     /locus_tag="OO145_004178"
     CDS             complement(2741..>2959)
                     /locus_tag="OO145_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5469072.1"
                     /translation="LRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTE
                     HASKIDAIISRNGTNTAQEENVVYLNAR"
BASE COUNT          702 a          750 c          830 g          677 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa
     2761 ccacgttctc ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc
     2821 ttgcgtgctc ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag
     2881 tttcccatcc tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc
     2941 ttgcccaggt atgcctcag
//
LOCUS       DAJTMX010000245         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTMX010000245 DAJTMX010000000
VERSION     DAJTMX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.245"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO145_004179"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO145_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5469073.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO145_004180"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO145_004180"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5469074.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO145_004181"
     CDS             2603..>2951
                     /locus_tag="OO145_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5469075.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTMX010000246         2945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTMX010000246 DAJTMX010000000
VERSION     DAJTMX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.246"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1245)
                     /locus_tag="OO145_004182"
     CDS             complement(<1..1245)
                     /locus_tag="OO145_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5469076.1"
                     /translation="MRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLE
                     LTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGT
                     IDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLAD
                     VTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGN
                     TAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTL
                     VEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYL
                     NNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLF
                     SGRWGTDGEWMLGAVGGYSDNQG"
     gene            complement(1321..2916)
                     /locus_tag="OO145_004183"
     CDS             complement(1321..2916)
                     /locus_tag="OO145_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5469077.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT          693 a          839 c          675 g          738 t
ORIGIN      
        1 gccctgatta tcactgtagc caccaaccgc cccaagcatc cactcgccat ccgtgcccca
       61 gcgcccgcta aacaagccgc cgctaagctg caccgtagaa gtgtcttcct gctgagccag
      121 ttgccctgcc gccgtgtaat gataacgtcc gccgataaca cgtaaattca gcgtctgacc
      181 atcgccacca gcgtgatcgc gtcgctccat cataaacgcc tgatttgccg cccgcaggtt
      241 attgagataa ccgccaactt tggcattcag caccggctgg taagcaggcg taggggccgg
      301 gtcgggggtt gggtcaggat ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg
      361 tggcgatggc ggcgttactt cttgcgatcg cagataccag tcgttgttat cttccactag
      421 cgtgtagtca tacgctccca tgttgacgta gccactgcca gccagactga actgtgcatt
      481 gttttgaaac tgcgtgggat cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg
      541 ctcaccaatc cctgtaatgg agttaaccac cacagttgtg ttgccagcag tattaccgtt
      601 catcaccaat tgatcgctta ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt
      661 accgccgccg gtataatcac cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag
      721 gctaaccgca ccggacgtat tggtcagatc accattaacc gtcacgtcag caagataggt
      781 caggctgtca gtttcaaggt tggtattaag ctcgcctgtc aacgtcacgc catcggtagc
      841 atttaccagc gtcccatcgc cgttaaatag tgaggcatta agggcgacgg aagtatcctg
      901 cccggtcaga cgcaaaaccg taccatcgct gatgtcgata gtgccgctgg aagtagcatc
      961 aattgactga atatcctgat ccgcgccagt aacgaacgtt gccccatcac caacccacag
     1021 tgacgaagca taaggaaaga tatccgcaac atcgcctttc agggtgcctt cttcgacacg
     1081 taccgccgac tgcgttgtac cgctggcggt cagctccagc gtacccgccc ccgttttagt
     1141 aaatgtgcta ccctcatcct gatgctgccc gctgctgtca gccatcagtg cgccccactg
     1201 cgtgtctacc cccgcatcaa ctgccacttc accgtcggcg cgcatttcaa tatcacgccc
     1261 atgctcagtc gtcgtagagc gagtatcgac attggcagta atctccatca cgctttcttg
     1321 ttacttatcg gtaaagataa cctggcggtt atagtgggta tctccaagct gcgagttgtc
     1381 gctcaccatc agcgtaccgg aggcgatttg tgttgtgccc aaataactat tattgtttgc
     1441 cagacttacc tgcccgtcgc tcgcatcgac caccacgtag acgccatctt tcccgtcgcc
     1501 gccgatatgc aaattgtcgc cacctgcaag atctaccaca tcctgactgc cgataaccgt
     1561 tccgctgctg ccactgactt caaggccatc attgtatgat gtgccgctct gccaagtgga
     1621 gaaatcagag agatcgagca caccgccgtt taacacgatc gactgcggat cgtcctggag
     1681 agcggtaaga tctgccgcgt cgccttccag cgaaagcacc gcaccatcat caacgactat
     1741 atcgccggtt agcgccatcg actgcgcccc tgccagcacg tagctgccgt tttgcgcaat
     1801 ggtgagctga ccagcacctt cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt
     1861 gccaccagca tcgatattta aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt
     1921 ttgttgggtc gagccaacgt ttagctcagc ctgattctga tactgatcaa tactccctat
     1981 cgtcagaccg tagcagtctt gcggatcgtc ctggcaatgc gtatcgccga cattcatcag
     2041 ggagttgctg cgacccaggg taacttcacc gttttcaatc tgcatctcac cagtaaagtc
     2101 attgttatct gcattaagta ccagatcgcc ggaacctgtt ttggtgatta acccggtacc
     2161 agcaatagag tcaacagctc cgtcattctc tgtattgccg ataaccagcg tttttccgtc
     2221 ggcaatatca aaggtaactt cacttaagcc gagatacata aagccacccg ccgcagtgga
     2281 gggaccatct ccatagcctg ctgcgctatt gttctcatcg actaacacgc cgccgttctg
     2341 gctgtagctg tcatcaacag aaatatcaat aagataggga gccgtcgcgc tattggtata
     2401 tatcgccccg ccataacctt cggctgtgtt atttgtaaag gcggtgttat ttattatcgt
     2461 ataacctgaa ggatgcttgc tgtcgctatt attatcggta acatcgattg ccccgccatc
     2521 gccatcactg taacttgttg atgtatatgc ctggttattg ttaaaaacat catcacttaa
     2581 atagacgtca ttattaatgg tataaattgc gccacctttg ccatcattag cgatattatt
     2641 gatggcgtta gtgacacgta aatcgacggc accagtatca ttagtaccag aagaatagat
     2701 tgcgccacca tagccgcctg cgacgttacc ggaaaaaata acatcagtaa gattcagcgt
     2761 tgagttttct ttagcaaata tggccccgcc gttattatat tctcctgtaa cggtgttatt
     2821 ggcaaacaga gtcattccag tttcattttc tggtaatagt gaaaattccg ctccttgttg
     2881 caaaaatacg gccccaccgc tggcattatt ggtcatatcc gaaaaaacca gccattgccc
     2941 atcgg
//
LOCUS       DAJTMX010000247         2932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTMX010000247 DAJTMX010000000
VERSION     DAJTMX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.247"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO145_004184"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO145_004184"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5469078.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO145_004185"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO145_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5469079.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="OO145_004186"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="OO145_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5469080.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2932
                     /locus_tag="OO145_004187"
     CDS             2753..>2932
                     /locus_tag="OO145_004187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5469081.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAJTMX010000248         2894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTMX010000248 DAJTMX010000000
VERSION     DAJTMX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.248"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..308)
                     /locus_tag="OO145_004188"
     CDS             complement(<1..308)
                     /locus_tag="OO145_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5469082.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO145_004189"
     CDS             complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO145_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5469083.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO145_004190"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO145_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5469084.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAJTMX010000249         2888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTMX010000249 DAJTMX010000000
VERSION     DAJTMX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.249"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="OO145_004191"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="OO145_004191"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5469085.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="OO145_004192"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="OO145_004192"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5469086.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="OO145_004193"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="OO145_004193"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5469087.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="OO145_004194"
     CDS             complement(1895..2809)
                     /locus_tag="OO145_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5469088.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          690 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacc
//
LOCUS       DAJTMX010000250         2839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTMX010000250 DAJTMX010000000
VERSION     DAJTMX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.250"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..806
                     /locus_tag="OO145_004195"
     CDS             <1..806
                     /locus_tag="OO145_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5469089.1"
                     /translation="QIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTIA
                     PFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASVL
                     GIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIRT
                     PSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCAD
                     GLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            820..1842
                     /locus_tag="OO145_004196"
     CDS             820..1842
                     /locus_tag="OO145_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5469090.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            1829..2824
                     /gene="yjfF"
                     /locus_tag="OO145_004197"
                     /pseudo
     CDS             1829..2824
                     /gene="yjfF"
                     /locus_tag="OO145_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
BASE COUNT          535 a          745 c          848 g          711 t
ORIGIN      
        1 cgcagatcga actggtaaaa atgatgctgg ggcgcgagct ggatacccac gcgctacagc
       61 gtgccgggcg aacattgttg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa
      121 aaggaacgat agcaccgttt gatctcgaag tgcgccccgg cgagatcgtc ggtctggctg
      181 gcttgctggg atccggacgt accgaaaccg ccgaagtgat cttcggtatc aaacctgctg
      241 acagcggcac ggcgttgatc aaaggcaaac cgcaaaccct gcgatcgcca catcaggctt
      301 cagtactggg catcggattc tgcccggaag acaggaaaac cgatggcatc atcgccgccg
      361 cctcggtgcg ggaaaatatc atcctcgctc tccaggccca gcgcggctgg ctacgaccca
      421 tttcccgcaa agagcagcaa gagattgccg aacgctttat ccgccagctt ggcattcgca
      481 caccttcaac tgaacaaccg attgaatttc tctccggtgg caatcagcaa aaagtgctgc
      541 tttcacgttg gctactgacc cgaccgcaat ttctgatcct tgatgagcta acccgtggca
      601 ttgatgttgg tgcccacgcc gagatcatcc gcctgattga aacgctgtgt gctgacggtc
      661 tggcgctgct ggtgatctcc tccgaactgg aagagctggt gggctatgcc gaccgggtga
      721 tcatcatgcg cgatcgcaaa caggtggcgg agatcccgct ggcacagctt tccgttccgg
      781 cgatcatgaa cgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tctccctgac
      841 actacgccgc caaaaaggcg ttttcactgg ccgacgggga tgccgcagct ggcggcactg
      901 ttgctggtgc tgctggtcga tagcctggta gccccgcatt tctggcaggt ggtgcttcag
      961 gatgggcgtt tgttcggtag ccctatcgac attcttaacc gtgcagctcc cgttgcgtta
     1021 ctggcgattg gtattacgct ggtgatcgcc accggtggta tcgatctctc tgtgggggcg
     1081 gtgatggcta tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg
     1141 attgttttgt taagcgccct gggcaccggc atcctggcgg gattgtggaa cggcatactg
     1201 gtagcgatcc tcaaaattca gccgtttgtc gccaccctga tcctgatggt cgccgggcgc
     1261 ggcgtggcgc aactgatcac cgccgggcag atcgtcacgt ttaactcgcc ggatctctca
     1321 tggtttggca gcggatcgct gttgttcctg ccaacgccgg tcattatcgc ggtgctgacg
     1381 cttctcctgt tctggctgtt gacccgcaaa acggcgctgg ggatgtttat cgaagtcgtt
     1441 ggtatcaaca ttcgggcggc aaaaaatgcc ggggtaaaca cgcgaatcat cgtcatgctc
     1501 acctacgtgt tgagcgggct atgtgcggcg attgcgggca ttatcgtggc ggcggatatt
     1561 cgcggtgccg atgccaacaa cgccggatta tggctggagc tggacgccat tctcgcggtg
     1621 gtgattggcg gcggatcgct gatgggcggg cgctttaacc tgctgctctc ggtagtgggg
     1681 gcgctgatta ttcaggggat gaacaccgga attttgcttt cgggctttcc gccggagatg
     1741 aaccaggtgg tgaaagcggt ggtggtgctt tgtgtgctga ttgttcagtc gcaacgcttt
     1801 atcagtctga ttaaaggagt acgtagccat gataaaacgt aacttgccgc tgatgatcac
     1861 catcggcgtc tttgtgctgg gctatctcta ctgcctgacc cagtttcccg gttttgcttc
     1921 cacaagggtg atctgcaata tcctgaccga taacgccttt cttgggataa tcgccgttgg
     1981 catgaccttt gtgatcctct ctggcggtat cgatctctcc gtcggttcgg tgatcgcctt
     2041 tactggcgtg tttctggcaa aagtggttgg cgatttcggc ctctcgccgc tgctggcgtt
     2101 tccgctagtg ctggtgatgg gctgtgcctt cggcgcattt atggggcttc tgatcgacgc
     2161 cctgaagatc ccggcattta tcattacgct tgcggggatg ttctttttgc gcggcgtcag
     2221 ctatctcgtt tcggaagagt cgattccgat aaaccatccc atttatgaca cgctctcaag
     2281 ccttgcgtgg aaaatccccg gcggcggtcg ccttagtgcg atggggctgt tgatgctggc
     2341 ggtggtggtt atcggtattt tccttgcgca ttgtacccgt tttggcaatc aggtgtacgc
     2401 tattggcggc aacgcgacgt cggcgaacct gatggggatt tctactcgca gcaccactat
     2461 tcgcatttat atgctctcca ccggactggc aacactggcg gggattgtct tctcgattta
     2521 tacccaagcc ggatatgtac tggcgggcgt aggtgtggaa ctggacgcca tcgcgtcggt
     2581 ggtgattggc ggtacgttgt taagtggtgg cgtcgggacg gtgcttggga cgctttttgg
     2641 cgtggcaatt cagggactga tccagactta catcaacttt gacggcacgc tgagttccta
     2701 gtggacgaaa atcgccatcg gtattttgtt gtttattttt atagcattac agcgtgggtt
     2761 aacggtgctg tgggagaatc gtcagagttc gccagtgaca agagtcaaca ttgcgcagcg
     2821 ataaaaatgc ctctccgtg
//
LOCUS       DAJTMX010000251         2801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTMX010000251 DAJTMX010000000
VERSION     DAJTMX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.251"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..2613
                     /locus_tag="OO145_004198"
     rRNA            <1..2613
                     /locus_tag="OO145_004198"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2709..>2801
                     /gene="rrf"
                     /locus_tag="OO145_004199"
     rRNA            2709..>2801
                     /gene="rrf"
                     /locus_tag="OO145_004199"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          729 a          619 c          879 g          574 t
ORIGIN      
        1 ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc
       61 acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg
      121 accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa
      181 aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc
      241 agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat
      301 attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg
      361 ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg
      421 ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg
      481 ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg
      541 tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg
      601 acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc
      661 gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag
      721 tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag
      781 cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg
      841 taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg
      901 ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta tcagaagtgc
      961 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
     1021 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
     1081 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
     1141 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
     1201 gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca
     1261 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
     1321 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
     1381 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg
     1441 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
     1501 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
     1561 taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
     1621 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
     1681 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
     1741 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
     1801 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
     1861 gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
     1921 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     1981 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     2041 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     2101 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     2161 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     2221 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     2281 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     2341 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     2401 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     2461 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     2521 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     2581 aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt ttggcggatt
     2641 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     2701 agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     2761 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca t
//
LOCUS       DAJTMX010000252         2778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTMX010000252 DAJTMX010000000
VERSION     DAJTMX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.252"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="OO145_004200"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="OO145_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5469091.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="OO145_004201"
     CDS             complement(983..1813)
                     /locus_tag="OO145_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5469092.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..>2778
                     /gene="intI1"
                     /locus_tag="OO145_004202"
     CDS             2023..>2778
                     /gene="intI1"
                     /locus_tag="OO145_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5469093.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHI"
BASE COUNT          622 a          673 c          692 g          791 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatt
//
LOCUS       DAJTMX010000253         2730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTMX010000253 DAJTMX010000000
VERSION     DAJTMX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.253"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(378..1095)
                     /locus_tag="OO145_004203"
                     /pseudo
     CDS             complement(378..1095)
                     /locus_tag="OO145_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1281..2123
                     /locus_tag="OO145_004204"
     CDS             1281..2123
                     /locus_tag="OO145_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5469094.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            2126..>2730
                     /gene="capU"
                     /locus_tag="OO145_004205"
     CDS             2126..>2730
                     /gene="capU"
                     /locus_tag="OO145_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5469095.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
BASE COUNT          704 a          594 c          625 g          807 t
ORIGIN      
        1 gtgtttcgca tacatgtcaa aacaccttcg accggcagtg cttacgtatc actgccagtt
       61 cctcctgcac ggcctgtcag atcacgtagg ccgtggtgac gttttcaacc cacagaggcg
      121 gattaacggg ttcatgttct tcagcctttc agtacttaac cttgaggatc atctcggctt
      181 agtgatctcg cctcaatccc cgttgtcagc gggttacatc accctgcggg catgccgcag
      241 gtcactgccg ctcaggttct ccaccgtcac acccggtggg attgttgggt ttctcatcgt
      301 gagttaccgg ttcaatattc cagacagact cgcggttcat ttaagcatcc atgcccgccc
      361 tgaactccgg gcacactata ggtcacatca ccgcaccaca cctgatttgg ttccgttacg
      421 gcgaactgtc gctcaagatg attcgggata gcaacgtgct catgaccgcc acgcttatac
      481 cggtgagtcg gctgctggca actgaccagc cccagctctt tcatgagtct gccagcaagc
      541 cagcgcccca tctggtagcc tctctgggtt gccattgtgg cgatgcttct tgctccggca
      601 gagccgtggc tgatgccatg cagttcaagt acctggctgc gtaatacagc ccgtctgccg
      661 tctggttttt caggacggtt tttccagtat ttgtagctgc tgcgatggac cccgaacaca
      721 tggcagagag tggccacagg ataacgcgcc ctgagtttcc cgattatcga aaactgttca
      781 gggagtctga catcaagagc gcggtagcct ttttaatatt tcattttcca tttcaatacg
      841 ttgtagcttt ttcctgagct cacggatttc aatttgttcc ggggtaatgg gggaggcttt
      901 tggtgttttt ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag
      961 gccgacatcc atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg
     1021 agcggattcg cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg
     1081 ttctgaggtg agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt
     1141 tcttagtgag ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt
     1201 ttgatttagt agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta
     1261 ctgggtgcca gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg
     1321 ttccggtatt aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg
     1381 agacattccg gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag
     1441 atgaactgga gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat
     1501 ttgatgatgg ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc
     1561 tcaaggcaca tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc
     1621 ctggaaagga atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata
     1681 atgttatgct tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc
     1741 atgttcatac acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt
     1801 gtaaacatct tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa
     1861 aatgcagtaa acatttgtgt tggccggaag gttattataa caaggattat attcagatcg
     1921 ctgaagaact tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta
     1981 aaggaactac acggatagga agaataagca ccaaggaacg ggaaaattgc gtatggctga
     2041 aaagacgact tttttattat acaacaccgt ttttttcatc gttgctggcc cttcataagg
     2101 ggccaagatt accggatgac taataatgaa tatactattt acggaatcat caccgaatat
     2161 aggtggtcag gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa tggggcattc
     2221 agttctgctt gtctgcaggg agaacagcaa aattgctttt gaagccagta agtttggaat
     2281 tgatatcaca ttcgcgttat ttcgaaacag tcttcacatt cctactgtat ggagattact
     2341 cggaatagtt catagttttc agcccgatgc aatcgtttgt cacagtggac atgatagtaa
     2401 tattgttggt ttggtacggt tttttacctg gaaacatcca ttcagaatta tcaggcaaaa
     2461 gacatatttg acacgaaaaa caaaattttt ttcaataaat catttttgcg atgaggtgat
     2521 tgttcccgga acaaatatga agacacatct ggagcaggaa ggatgtcgaa ctcgggttac
     2581 tgttgtgcct cctggctttg atttccagga attatacgtt gattcccgaa acagtttgcc
     2641 tccgagtgtt ctttcctggc tggcgtcccg aagaggatgc cccgtaattg ctcaggtagg
     2701 aatgttgcgc ccggaaaaag ggcacgaatt
//
LOCUS       DAJTMX010000254         2725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTMX010000254 DAJTMX010000000
VERSION     DAJTMX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.254"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            64..2586
                     /locus_tag="OO145_004206"
     CDS             64..2586
                     /locus_tag="OO145_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5469096.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2597..>2725
                     /locus_tag="OO145_004207"
     CDS             2597..>2725
                     /locus_tag="OO145_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5469097.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          729 a          589 c          681 g          726 t
ORIGIN      
        1 gccagtctag tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt
       61 attatgatga cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg
      121 caaaacctga tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa
      181 tccggagtcg atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc
      241 gttgatattt ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat
      301 aatccagatg ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt
      361 ggcataaaag ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat
      421 cctgctccac gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat
      481 atatccattc accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt
      541 tgggatgagg ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta
      601 cgttcagatt atggttcaca agagacagat accagttatc tcaatctgcg caatggactg
      661 aatattggac cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg
      721 gaatacaact ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt
      781 atgattggtg atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg
      841 cgtttgtata ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt
      901 cgtgggattg caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat
      961 cagtcagccg ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt
     1021 ggcgatttgg acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct
     1081 tatgcttcat tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt
     1141 gaattgcgcg atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt
     1201 ttttcccacg ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca
     1261 gctctgggtg tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat
     1321 gctcgtgcga attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat
     1381 tcaaaactat ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc
     1441 gagggctact ataccctcaa tgagtgggca tcgcggcgca acagccctga agacttttgg
     1501 gaaacaggta accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat
     1561 tatggcaatt tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa
     1621 cgattaatgc aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg
     1681 agttattcca atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt
     1741 gagcagatct acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac
     1801 gccacctatt ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc
     1861 ggtacggcgc tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat
     1921 gatgatgagg ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac
     1981 ggcagctata actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt
     2041 ctggcacaca gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt
     2101 aaagcacctg gggccgccag gttagaaata gataatatgc gcggtgctgc gacggactgg
     2161 cgcggctata cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc
     2221 gataactatt tctcgaagtc gaatatagaa cttgataata ccgtcgttac gatggttccc
     2281 acgcgtggtg cagtggttaa agcggagttt gtgactcatg tgggttatcg cgttctcttc
     2341 agagtgttaa atgcaaatgg taaaccggta ccttttggag ccattgctgc gatacaagat
     2401 gcaagtttgg cagattcagg aattgtcggt gaccgtggcg aactttatct ttctggtcta
     2461 ccagaaaaag gacaggttac gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc
     2521 aattattcat tttcgacacc agaaagtgag agcggattaa ttgaacaggg tgtgacatgt
     2581 cattaaaagg atttttatga acaagtatat caaacagtgg tgctttgctg tgtttatgct
     2641 ctcgttaagt agtgtagcac ttgcagctcc taaaggtatc tgtacccctg ataatggcgt
     2701 atttcacagt acgcttgatt tctcg
//
LOCUS       DAJTMX010000255         2575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTMX010000255 DAJTMX010000000
VERSION     DAJTMX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.255"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            119..1282
                     /locus_tag="OO145_004208"
     CDS             119..1282
                     /locus_tag="OO145_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5469098.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1632..1994
                     /locus_tag="OO145_004209"
     CDS             1632..1994
                     /locus_tag="OO145_004209"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR5469099.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1991..>2575
                     /locus_tag="OO145_004210"
     CDS             1991..>2575
                     /locus_tag="OO145_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5469100.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVR"
BASE COUNT          793 a          541 c          605 g          636 t
ORIGIN      
        1 atcccactaa gaataaatct ccttacctca caacacgaca agcctgcatt gcggctcttc
       61 agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag gaaagaaaat
      121 gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga acggtaaaag
      181 atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc atgacaagct
      241 aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa cgttcgagga
      301 agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg atgacaaaag
      361 ccggatcgga ttctggcttc aacatttcgc aggaatgcaa ctaagagaca ttactgaatc
      421 aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa actggagact
      481 cagggcagaa gcatgcagaa aaaaagggaa acctgttcca gaatacacgc caaaaccagc
      541 gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa gagccgcaga
      601 gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac caaagaataa
      661 acggatccgc tggctggagc cccatgaagc acaaaggctg attgatgaat gtccggagcc
      721 attaaagtct gttgttgaat ttgcactggc aacaggctta agacgctcga acatcatcaa
      781 ccttgaatgg caacaaatag atatgcagcg ccgggtggca tggataaacc cggaagagag
      841 taaatcaaac cgcgcaattg gcgttgcgct gaatgatact gcatgtcgcg tattgaaaaa
      901 acaaatcggg aatcatcacc gttgggtatt tgtgtacaag gaaagctgta ccaaaccaga
      961 cggaacgaaa gcgccaacgg tcaggaagat gcggtatgac gcaaacacag cctggaaagc
     1021 ggcgctgaga cgagcaggta ttgatgattt cagatttcac gacttgagac acacctgggc
     1081 aagttggctg gttcaagccg gagtcccgtt gtcagtgtta caggaaatgg gaggctggga
     1141 gtctatcgaa atggttcgtc gatatgctca ccttgcacct aatcacctta ccgaacacgc
     1201 acggcaaata gactcgatcc tgaacccatc ggtcccaaat tcgtcccagt caaaaaataa
     1261 ggaaggtact aatgatgtgt aacttattga tttaaatggt gccgataata ggagtcgaac
     1321 ctacgacctt cgcattacga atctgtagca ccaatcataa ctatctgttt tagcaagcat
     1381 taaccgcatt cactaagata tagttgatgg cacaaacaga aagttgatga atgatgttgc
     1441 catatatatg tcacaaatac ggcacaacga tcttcaaaca tatagccaca cattcacaga
     1501 agagcacaaa gccttgcaat ccagtgcaaa gatttgtgtg ccagagtttt tctcatcaac
     1561 taccgcaagt atcgatcgat tgagacttgg atgatagact tcatgccttt gattattagc
     1621 tgatagaaga aatgttaaag ctatttgcaa agtacacatc tataggcgtg ctgaacacac
     1681 ttatacactg ggtggttttt ggtgtatgta tctatgccgc gcataccaat caggctatgg
     1741 caaacttcgc aggtttcgtt gtggctgtga gttttagctt ctttgcgaat gcaaaattca
     1801 cattcaaagc atcaactaca acgatgcgct acatgttata tgtcgggttc atgggaacac
     1861 tgagtgctac tgttggatgg gttgctgata gatgttcact tcctccaatt gtcactcttg
     1921 ttaccttctc cgccatcagc ttggtgtgcg gtttcgtcta ttcaaagttc attgtcttta
     1981 gggatgcgaa atgaagatat ctcttgtcgt tcctgtcttc aatgaagaag aagcgatacc
     2041 aattttttat aaaacggtac gtgaattcga agagttgaag ccatatgaag tagaaattgt
     2101 tttcataaat gacggaagca aagacgctac ggagtcaatc attaacgctc tggctgtttc
     2161 tgatccgcta gttgttccgc tgtcatttac acgcaacttt ggtaaagaac cagcattgtt
     2221 tgcagggtta gaccatacaa ccggcgatgc cgtgattcca attgatgttg acctgcaaga
     2281 cccgattgag gttattcctc atcttattga aaagtggcag gcaggtgctg acatggtgct
     2341 tgctaaacgt tcagaccgct caactgatgg acggctgaaa cgtaagacag ctgagtggtt
     2401 ctataaatta cacaacaaaa taagcacccc aaaaatagag gaaaatgtcg gagattttcg
     2461 actcatgtct cgtgaggttg tggagaacat taaactattg cctgagcgca atcttttcat
     2521 gaaaggcata ctgagctggg ttggtggtca gacagatgtc gttgaatatg tacgc
//
LOCUS       DAJTMX010000256         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTMX010000256 DAJTMX010000000
VERSION     DAJTMX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.256"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(64..822)
                     /locus_tag="OO145_004211"
     CDS             complement(64..822)
                     /locus_tag="OO145_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5469101.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO145_004212"
     CDS             complement(830..1933)
                     /locus_tag="OO145_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5469102.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO145_004213"
     CDS             complement(1943..>2572)
                     /locus_tag="OO145_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5469103.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTMX010000257         2487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTMX010000257 DAJTMX010000000
VERSION     DAJTMX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.257"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>2487
                     /locus_tag="OO145_004214"
     CDS             <1..>2487
                     /locus_tag="OO145_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5469104.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAJTMX010000258         2463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTMX010000258 DAJTMX010000000
VERSION     DAJTMX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.258"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(746..2350)
                     /locus_tag="OO145_004215"
     CDS             complement(746..2350)
                     /locus_tag="OO145_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5469105.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          576 c          526 g          713 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 accccttgac aagtagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAJTMX010000259         2454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTMX010000259 DAJTMX010000000
VERSION     DAJTMX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.259"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..377
                     /gene="capU"
                     /locus_tag="OO145_004216"
     CDS             <1..377
                     /gene="capU"
                     /locus_tag="OO145_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5469106.1"
                     /translation="SMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEAS
                     AFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQ
                     DIEERFDINKTALKILTLAKQK"
     gene            382..1332
                     /gene="virK"
                     /locus_tag="OO145_004217"
     CDS             382..1332
                     /gene="virK"
                     /locus_tag="OO145_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5469107.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1397..2341
                     /gene="lpxM"
                     /locus_tag="OO145_004218"
                     /gene_synonym="msbB"
     CDS             1397..2341
                     /gene="lpxM"
                     /locus_tag="OO145_004218"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5469108.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          712 a          473 c          554 g          715 t
ORIGIN      
        1 acagtatggg catgcatgat gatgttttta ttgctgacaa tgtttttcct gccgcccccg
       61 tatatcgggt tgccagtctg gtggttctgc cttcagaaaa cgaatctttt ggtatggtgc
      121 tggcagaagc atcggcattt tctgtgcctg tagtggccac tcagattggt ggaatccctg
      181 aggttattca gaacaaccag accgggacat tgttaccagc aagtaataag cacgcatgga
      241 tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt ttatcagatg gctcgcctgg
      301 caaaacagga tatagaagag cggtttgata ttaataaaac tgcgttaaaa atactcacat
      361 tagcgaagca aaagtaacaa tatgttttct ataagtaact tatcatttat cggtttcctt
      421 aaaaggatta ttttttcctc agattcacta ccggggaagt gggaacacag aaaatttcgg
      481 ttcatgtaca ttttgcgatg ttctataaat ccggttgtca gtattcgata ttattatgaa
      541 ctgcgttcct tgccgtgcat tgaggatatt ctggcaatac accccacgtt gccagcaaga
      601 attcatcgcc cttatttgca taaagggggg cgcgcctgga cccgtggaca atatattctt
      661 gagcattatc gctttgtgca aaatttgcca gaaaaatatt ctaaattttt gtttccacaa
      721 aaatcggtat cattagtcca attcatcggg aaagatggag agaattttga tatccagtgt
      781 tctcccagtg gttttgaccg ggaaggagag ttgatgttgt cattgtttta caataaaaca
      841 gttattgccc ggttaacatt ttcagtcatt ctgactcaaa atggacacat tgcttttatt
      901 ggcggattac agggagcccc taaaaatacc ggacctgatg ttattcggtg cgcaacccgg
      961 gcctgttatg ggattttccc aaaacggatt atttttgaag cattttgtgc gttgatgaaa
     1021 gcttgcaata tctctgagtg tctggcagtc agtgagcaca gtcatgtttt ccggcaattg
     1081 agatactggt atcagaaacg caaaaccttt gttgcggtct atagtgattt ctgggagtct
     1141 gtagcaggaa aaacctgtgg tgactggtac agattaccaa cccaggtgat tcgaaagccg
     1201 ctaagcgata tagccagtaa aaaacgctct gagtaccgaa aacgatacgc tttgctggat
     1261 tatatccatg aaactactat ccgctctttg gatgcatatc ctgtacactc agaacatcag
     1321 aatttaaatt aaggtttgat gtattctctg aataaaacag taatgataat tttggtagag
     1381 gcattcgcac taaataatga aaaaatataa atctgagttt attcctgaat ttaagaaaaa
     1441 ttacctttcc cctgtttact ggtctacatg gttccttttg ggaatgattg caggtatttc
     1501 aatgtttccc ccttcattca gagatcctgt cttggcaaaa atagggcgtt gggcgggcag
     1561 attgagcaaa aaagctcgtc gcagggcgac gattaattta tcgctttgtt tcccagaaaa
     1621 gagtgataca gaacgggaaa taattgtcga caaaatgttt gtcacagcat tgcaatccat
     1681 agtgatgatg gcagaactgg caattcgtgg tccggaaaag ttccagaaac gagtgttctg
     1741 gaaaggcctc gaaattcttg aggaaattcg acacaataat agaaatgtga tttttctggt
     1801 tccccatggc tggagcgtgg atattcctgc aatgttgctg gcagctcagg gaaaaaaaat
     1861 ggccgccatg tttcatcagc aacgaaatcc agtgattgat tatgtctgga attcagtacg
     1921 gcgtaaattc ggggggcgct tacatgcccg ggaggatggg ataaaaccat ttattcagtc
     1981 agtacgccag ggatactggg gatattacct cccagatcag gatcatggtc ctgaatacag
     2041 tgaatttgct gatttttttg cgacctataa agcgacatta ccaatcattg gacgtctgat
     2101 gaacatcagt caggctatga ttataccact tttcccggtt tatgatgaaa aaaaacatct
     2161 actgactatt gaaattcggc caccaatgga tgcttgcatt gccagcgcgg acaataaaac
     2221 gattgcccga caaatgaaca aaacagtgga aattttggtg gggccacatc cggaacagta
     2281 tgtctgggtt ttaaaattgt taaaaacacg caaatcaaac gaagcggacc cgtacccttg
     2341 aaattataaa gtacaagtat ttccactagt tgtttgtaag cgtacagcct gaaccgtctg
     2401 gtcagaatct gacgaattag acaaagtggt gtccaccaaa taagtagtgg gaac
//
LOCUS       DAJTMX010000260         2439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTMX010000260 DAJTMX010000000
VERSION     DAJTMX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.260"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..565
                     /locus_tag="OO145_004219"
     CDS             <1..565
                     /locus_tag="OO145_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5469109.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="OO145_004220"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="OO145_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5469110.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO145_004221"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO145_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5469111.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="OO145_004222"
     CDS             2167..2403
                     /locus_tag="OO145_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5469112.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DAJTMX010000261         2345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTMX010000261 DAJTMX010000000
VERSION     DAJTMX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.261"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..797)
                     /locus_tag="OO145_004223"
     CDS             complement(<1..797)
                     /locus_tag="OO145_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5469113.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1396..1644)
                     /locus_tag="OO145_004224"
     CDS             complement(1396..1644)
                     /locus_tag="OO145_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5469114.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1999..>2345)
                     /locus_tag="OO145_004225"
     CDS             complement(1999..>2345)
                     /locus_tag="OO145_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5469115.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          607 a          431 c          562 g          745 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag ataattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggtg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 gggcaatttt tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc
     1081 cactggtatc agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg
     1141 ttttttattc ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg
     1201 tcggtgtttt gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc
     1261 aatcatgatt ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt
     1321 gctgcggctg gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact
     1381 gattttacgt gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag
     1441 agtttcctga acaaaagttt tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc
     1501 atcgttgctg gtggctttta cgatcacctc tacatcgtca taacgtttac tgatgcgtcg
     1561 ggttaattct tcctttaacg catccacagc accggttggc attttagtca ttttctcttt
     1621 ggctatgcag atttcaaaac gcataaaaat ccctctgcac tgtgttttta tacagtatta
     1681 tttttaactg tatggataaa cagtgtcaag agtgagggaa agtgattttt tggggcatgc
     1741 atggggcaaa aatatttgtt gtggggcatt ttggggcatg attgggacac tttgttacat
     1801 atgaactttg ccgattttca tatgacatgg caatccaacg ctttgattat tctaaagaaa
     1861 acacatgctc ttgggcgttc tttagtgatt tttaaaaatc ccgtatcacg cagttaaagt
     1921 ggcgggcata ctcttcaagg ctggtgatcc ccaaccgcac ccatttagga tgtgaccatt
     1981 gcggtagacc gatgtaaatc ataacgcgtg aaggatctct tccacgctac gcacacgggc
     2041 gatgcgcggg tagatatgat taatgctgtt attgtgttgc tcggcgctgg cggcactaca
     2101 ggcgtcttcg gcaatcacca gattaaagcc aagttcccag gcattgcggg cggtggattc
     2161 aacaccgata ttggtcgaga tcccacataa cactattgta tcgatacctc ggcggcgcag
     2221 ttgcaactcc agatccgtac cgtaaaacgc accccactga cgtttgatga tttcgatatc
     2281 actgtcagtt gcacctaatg cagctggatg ttgccaccag ttttcgggca acactttagc
     2341 ggggg
//
LOCUS       DAJTMX010000262         2339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTMX010000262 DAJTMX010000000
VERSION     DAJTMX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.262"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(2..94)
                     /locus_tag="OO145_004226"
     tRNA            complement(2..94)
                     /locus_tag="OO145_004226"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO145_004227"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO145_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5469116.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..>2339)
                     /gene="alaS"
                     /locus_tag="OO145_004228"
     CDS             complement(830..>2339)
                     /gene="alaS"
                     /locus_tag="OO145_004228"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5469117.1"
                     /translation="LALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNI
                     KVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVD
                     GKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHI
                     GKLAAGSLKVGDAVQADIDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDK
                     VLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMNLEAAKAKGAMALFGEKYD
                     ERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVH
                     ADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAI
                     DVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGSTIIVLATVAEGKVSLIAGVSKDVTD
                     RVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ"
BASE COUNT          578 a          659 c          523 g          579 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccaga
//
LOCUS       DAJTMX010000263         2325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTMX010000263 DAJTMX010000000
VERSION     DAJTMX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.263"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="OO145_004229"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="OO145_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCR5469118.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="OO145_004230"
     CDS             276..452
                     /locus_tag="OO145_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469119.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="OO145_004231"
     CDS             527..802
                     /locus_tag="OO145_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469120.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="OO145_004232"
     CDS             902..>2325
                     /locus_tag="OO145_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5469121.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAJTMX010000264         2244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTMX010000264 DAJTMX010000000
VERSION     DAJTMX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.264"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(158..670)
                     /locus_tag="OO145_004233"
     CDS             complement(158..670)
                     /locus_tag="OO145_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5469122.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            865..1629
                     /locus_tag="OO145_004234"
     CDS             865..1629
                     /locus_tag="OO145_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469123.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1580..>2244
                     /locus_tag="OO145_004235"
     CDS             1580..>2244
                     /locus_tag="OO145_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5469124.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          643 a          490 c          565 g          546 t
ORIGIN      
        1 atcatcagcg accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt
       61 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
      121 ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      181 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      241 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      301 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa
      361 cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa
      421 ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga
      481 ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa
      541 ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa
      601 aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc
      661 actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt
      721 aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag
      781 cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa
      841 cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca
      901 taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg
      961 gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc
     1021 aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt
     1081 acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga
     1141 atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc attaaaacac
     1201 ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg
     1261 acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt
     1321 ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt
     1381 gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc
     1441 gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg caaggccgcc
     1501 actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat tgatgatggc
     1561 aatggagagc gtgacctcaa tgacttctac tctgacatcc aaaccgacgc tgaatccggc
     1621 tttacgtagt ttctggacta tgcgggcacg taacaaagtg ctttatggtg gtcggtcatc
     1681 gtcaaaatca tgggatgccg ctggcattgc catatttctg tcgaataaat acaccctgcg
     1741 tttttgttgt gcccgtcaga tccagaataa aatcgaagag tcggtgtata ccctgctcaa
     1801 aattcagata gacaggtttg gtctgcggca ccgtttccgt attctgaaca acaaaatcat
     1861 taaccgggtt actggctcgg aatttgtttt ttatggatta tggcgcaaca tcgaagaaat
     1921 taagtcactg gaggggatcg atatgttgtg gctggaagaa gcccacgcac tgacggaata
     1981 ccagtggaaa attctggagc caacgatccg taaagagggt tcggaatgct ggttcatatt
     2041 caaccccgga cttgttactg atttcgtctg gcgcaacttc gttgttgatc cgcccgaagg
     2101 cactctcatc cgcaaaatta actatgacga aaatccgttt ctgtctgaca ccatgcttaa
     2161 ggttatcgac gcggcgcgac gccgtgatcc ggatggtttt aaacatgtgt atgagggcgt
     2221 tccggagtct gatgatgatg cggc
//
LOCUS       DAJTMX010000265         2218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTMX010000265 DAJTMX010000000
VERSION     DAJTMX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.265"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO145_004236"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO145_004236"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5469125.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO145_004237"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO145_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5469126.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="OO145_004238"
     CDS             complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="OO145_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5469127.1"
                     /translation="IQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAJTMX010000266         2187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTMX010000266 DAJTMX010000000
VERSION     DAJTMX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.266"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(220..384)
                     /locus_tag="OO145_004239"
     CDS             complement(220..384)
                     /locus_tag="OO145_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469128.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OO145_004240"
     CDS             1286..1570
                     /locus_tag="OO145_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5469129.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OO145_004241"
     CDS             1558..2043
                     /locus_tag="OO145_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5469130.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAJTMX010000267         2183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTMX010000267 DAJTMX010000000
VERSION     DAJTMX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.267"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..694
                     /locus_tag="OO145_004242"
                     /pseudo
     CDS             1..694
                     /locus_tag="OO145_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            696..1301
                     /locus_tag="OO145_004243"
     CDS             696..1301
                     /locus_tag="OO145_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5469131.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="OO145_004244"
     CDS             1288..>2183
                     /locus_tag="OO145_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5469132.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAJTMX010000268         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTMX010000268 DAJTMX010000000
VERSION     DAJTMX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.268"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            80..259
                     /locus_tag="OO145_004245"
     CDS             80..259
                     /locus_tag="OO145_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469133.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO145_004246"
     CDS             311..505
                     /locus_tag="OO145_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469134.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO145_004247"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO145_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAJTMX010000269         2151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTMX010000269 DAJTMX010000000
VERSION     DAJTMX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.269"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO145_004248"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO145_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5469135.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO145_004249"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO145_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO145_004250"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO145_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          630 a          398 c          492 g          631 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cataacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAJTMX010000270         2141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTMX010000270 DAJTMX010000000
VERSION     DAJTMX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.270"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..275)
                     /locus_tag="OO145_004251"
     CDS             complement(<1..275)
                     /locus_tag="OO145_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469136.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVT"
     gene            complement(728..1990)
                     /locus_tag="OO145_004252"
     CDS             complement(728..1990)
                     /locus_tag="OO145_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5469137.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
BASE COUNT          565 a          497 c          493 g          586 t
ORIGIN      
        1 gtaacaatat caggcgcgcc tgcggaacgg tccttaccat gctgcagtaa ccggaaataa
       61 acttttgggc gcaagatgac atcgaccgtt tcgacatcgt ctttttctcc ctcaaaaaat
      121 ttactgacaa tcgcttcgtc aagacgtccc gccgctatcc catgccagtg agtgaaattt
      181 tgggcgtctt tccgtttaaa actgcccttt cctgactcag catccgcaag cgagttgcgc
      241 cagtatgagg caaaccctaa agcattttca tccattacca gtcctgtgac tcaatgaaat
      301 ccattgattt atcgaatata aaatcaccgt atggacagct attgtccata ttgaaaaagt
      361 tggatgcaat gttttgttta taattcgata tcaaatgaac tggttgtaca aggttcagaa
      421 cacaagctca ttaaacagta cgtcaggact attgatttca taacagtgaa tacatttttt
      481 accgtttata ataactacct gtgttggacg gggataccag agcagccgaa aacaccgggg
      541 gttattacct gctctcatgg aaaccatata ttcctggtgc taatcacagt cataaatagc
      601 ctgaagaacg tgacgaaggg actttaatgg aagccggagt aacttgtcag aaacatcaat
      661 cttcgtcatc atttacaaag aaaaataatt agttaaagca gtccgggctg attgcccgga
      721 ctgcttacta ttttaatgga ttgttaatct ttgcatattc aaacgggctg atacccttct
      781 cccggttcgc atctagaaaa tcggcccacc attgcaacat cagtcgccgt tcttccagat
      841 gttctgcttt atggatatac gcagcacgta caccattacg ctcctgatgg ctcatctgac
      901 gttccacagc atcgcgggac cacaaacctg attcaatcaa tgcactgcac gccatcgccc
      961 ggaatccatg gccacagact tcctgggttg tgtcataccc catcaccctc agggctttat
     1021 ttacagtatt ttcactgatt ggtttacgcg ggtcatgtgc acccgtaaag atcagaccat
     1081 tttcaccagc ccatgtctga agttctgcca gtatggctac aacctgtttg ctgagtggca
     1141 caagatgtgg tgttcgcatc ttggatcccc ggtgtgagaa ttttacgcct ggcagaggtt
     1201 cgcgttcggc tggaatagtc cacatggaat tgtcaatatc aatttccgac cagcgggcat
     1261 atctgagttc actggaacga ataaaaacca gtaaattcag cataactgcc agtcgggtga
     1321 caggctggcc tttataaccg tcaatacgcg acagaagttc aggcaggcgt gaaagctcca
     1381 gagcagggcg atgctggcgt tttaccgtgg tcaatgcacc agccatatcc agagccgggt
     1441 tatatctgat gatgccagac tgtacggcgt aacgcattac agcagatatt ctttgctgca
     1501 gacgactggc gatttcataa atttgttttg cttctgcagc acgaacaggg ataagcaggt
     1561 ccggggtatt gagtgttgtg atatcccggt tgccgataaa ggggaaaacg tgtgtctcga
     1621 ggcttttgag tactttatcg ctgtgtgatt gtgcccattg tttattactg gcatgccagc
     1681 gtcgtgcaat atgttcaaaa gagagctgta aatcagggac aactttttta actcttttct
     1741 gttcgcaagg gtcaatcccc tctgaaatca gttttcgggc ctcatcccgt tttgtacgag
     1801 cttcggagag agaaacttct ggatacacac caagtgccag agtcttctcc ttacccagaa
     1861 tacgataacg gagacgccag taacgggaac cattaggatg aaccagcagg aacatgccgg
     1921 ccccgtcagt gagtttataa gccttgtcag tgggctttgc agcccggatt tttgcgtcag
     1981 tcagtgccat agttcagcct tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg
     2041 ggtatacccg caattatacc cacacaatgg ggttgatgtg gggggaatct ggttgagttc
     2101 ggtagaattg atttggagga agaacattta aatcatggtg t
//
LOCUS       DAJTMX010000271         2083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTMX010000271 DAJTMX010000000
VERSION     DAJTMX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.271"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..795
                     /locus_tag="OO145_004253"
                     /pseudo
     CDS             1..795
                     /locus_tag="OO145_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            910..1251
                     /locus_tag="OO145_004254"
                     /pseudo
     CDS             910..1251
                     /locus_tag="OO145_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1234..2071
                     /locus_tag="OO145_004255"
                     /pseudo
     CDS             1234..2071
                     /locus_tag="OO145_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          503 c          630 g          435 t
ORIGIN      
        1 ggccttgccc gtagcagtta tttttatcac cgggctgcac tgaaagccgg tgataaatac
       61 gccacgatac gtaagatgct gacagatatc tttaacagta attaccagtg ttatggctat
      121 cgtcgcctgc atgcgatgct caggcatgag ggggggcggc tatcagaaaa ggttgtacgc
      181 agacttatgg tggaggaaca gcttgtcgtc agtcgtaacc gtcgtcgtcg ctacagctca
      241 tattgcggag aaatcggacc ggctccggat aaccttatcg ccagagattt taaggcggag
      301 caacctaatc agaaatagct gacagatatc acggagttcc agctccctgc aggtaaagtc
      361 tggctgtcac cagtggtgga ctgcttcgat ggaaaagttg tgagctggtc tctcagtaca
      421 cgccctgatg ctgaactggt caacactatg ctggatagcg ctgtcgaaac gttaaatgct
      481 ggcgaacgac cggtgataca cagtgacaga ggtgggcatt atcgctggcc aggctggctg
      541 gaaagagtga atgcagcagg tcttattcgc tcaatgtccc gtaaaggatg ttcacctgat
      601 aatgctgcat gcgaaggctt tttcggcaga ctgaaaacgg aaatgtatta tgggcgtaaa
      661 tggtcgggca tcacgccaga aaagttcatg cagcaagtgg atgcttacat cagatggtat
      721 aacgagcggc gtataaaatt atcgctgggt gccgtcagcc ccaaaatgta ccgccaacaa
      781 tgcgggctgg aatgataaag cagtccagga aatcgtccgc atcccccctt caccatttgg
      841 attgtccgct ccacccaaca tgttgtttcc ttaaggttcc tacaccacaa aggaaactct
      901 catgctgcgc agtggatggc tgacgttgcc gacaggcggg aacttcgcca gttcaggcag
      961 acaccggaac agcgcttcac gcaggaacag gagcatctgc agccgctgct gggtacggac
     1021 ttcgatatcc gtcatgtctc ctgggatggc tatatcgagg ttggcgggaa ccgttacagt
     1081 gtgccggaaa gcctgtgtgg tcagctggta tcaatacgga tctcgctgga tgatgaactg
     1141 cggatctaca gtaatgagca acaggtagca tcgcaccgtc tctgttcggc tgcatacggc
     1201 tggcagaccg tgccggagca tcacgctcca ctctggcagc aggcttcgga gcgcatggtc
     1261 gaatggctgg gggagataca gaaacgggta atcactgtct gtgaccggga agcggatatc
     1321 tggcattacc tgtattataa agtcagccac ggacagcgtg gtgcgtgctg cacagaatcg
     1381 ccggctggaa gaggcacccg gcaagctctt cgaactgccg gaagtcctgg caaccgcagg
     1441 aagccacacg ctgaatgtga tgcaaaaagg cgggcgggca gcccgtcagg cccggatgtt
     1501 catcagttac agcgaagtca gcataaaaaa tcccgacaac agcggccagg cgctcccgct
     1561 cacgtatgtc tgttgccggg agcaggcaga ggacggtgcc tgctggcatc tgctgacgtc
     1621 aggaaaagcg gcgagtgccg ccgatgcacg acgtattgtc agccattacg agcgacgctg
     1681 gctgacagag gaataccaca aggcgtggaa aagtggtggt acatggaatc gctgcgaatg
     1741 cagacccggg ataaccttga gcgcatggtg gttatcctgg cgtttatagc ggtacgggtg
     1801 ctgggcctgc ggcagggagg tgtcagtgaa gaaacacaaa atgacagctg tgagaaaata
     1861 ctgacgccga cagagtggaa actactgtgg gttaagctgg aaggaaagcc gcttccggtg
     1921 caggcaccga ccctgaaatg ggcctggggc gatggcatga cagcaaacgc acaggtcgtc
     1981 ccggttgggg cgtcatgtgg gatggctggt cagacttcag gatatggttg agggttacct
     2041 ggtgatgaag tctcttgatc aggagctctg atcaagagac agc
//
LOCUS       DAJTMX010000272         2047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTMX010000272 DAJTMX010000000
VERSION     DAJTMX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.272"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            6..224
                     /locus_tag="OO145_004256"
     CDS             6..224
                     /locus_tag="OO145_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5469138.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO145_004257"
     CDS             226..591
                     /locus_tag="OO145_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5469139.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO145_004258"
     CDS             588..1253
                     /locus_tag="OO145_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469140.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO145_004259"
     CDS             1253..1618
                     /locus_tag="OO145_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5469141.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          558 a          423 c          543 g          523 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgt
//
LOCUS       DAJTMX010000273         2036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTMX010000273 DAJTMX010000000
VERSION     DAJTMX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.273"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..2036
                     /gene="sat"
                     /locus_tag="OO145_004260"
                     /pseudo
     CDS             1..2036
                     /gene="sat"
                     /locus_tag="OO145_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          748 a          357 c          400 g          531 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcagat
//
LOCUS       DAJTMX010000274         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTMX010000274 DAJTMX010000000
VERSION     DAJTMX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.274"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..362)
                     /locus_tag="OO145_004261"
     CDS             complement(<1..362)
                     /locus_tag="OO145_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5469142.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="OO145_004262"
     CDS             complement(429..1643)
                     /locus_tag="OO145_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5469143.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          492 c          413 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DAJTMX010000275         2029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTMX010000275 DAJTMX010000000
VERSION     DAJTMX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.275"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..1823)
                     /locus_tag="OO145_004263"
     CDS             complement(<1..1823)
                     /locus_tag="OO145_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5469144.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1931..>2029)
                     /locus_tag="OO145_004264"
     CDS             complement(1931..>2029)
                     /locus_tag="OO145_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5469145.1"
                     /translation="RWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 cgcagttctt cacttgctga ggaaaaaaca agcgttccat ccttctcgac atacccatcc
       61 gtttcaagca gaccacacag catgcctata cgcgttgatt tatttgcgga aaaaaacaca
      121 cgagggatga atttgttttt tgctgtgcac cccattagtc cataacctcg cagggtctcc
      181 atgagtggat ttacctgacc acgcgccgtt gatatcagcc agtccttacc atctccagag
      241 aagttgtaat caggcagttc agccttcata cgctcgatca tgtatggctc gacgtttgaa
      301 aacttcacgc tcccattgct aagactacca tccccgagaa gggagccgat aacccatgct
      361 aatggctctg agtggtcgcc aaagtcacca gttatttcag gaatcctaac gccatttttg
      421 tgcctcttcg tttcactcaa ccgcttcaac ccgaaggtat ctatcacgcg gcgtttctcg
      481 cctttcgtga atccacgact agtgacctcc cataggtgat cgcctgcgca atcaacataa
      541 cgaccatctt caaatgtgac tcggtaaaca tctctaacac cctgcgggaa tatcccagtg
      601 acagttgacg gattgccgtc tactgacgcc acctgatcgc caatttttac attcccgtga
      661 gtagtccatg tcccatcagc taaaagaata ggctcattca gtgggcatgc ctttccgtga
      721 ccgtgcacac cattgaccag cgtcaactct gcctcacgga actggaattt atctgccaga
      781 gattcccacg gtggattaaa cagatactgc tgcttgccgt agaaagcgtt gatagtgtcc
      841 tggtaaaact ctcgcgcact gtagagttct tcaggatcga agtaggatgc cgtgccgatg
      901 tactgccaga tttcatcctc ggtaacaccg ttcatcaggc attcgttgat gtctttgtac
      961 ggcagagtaa caagacggca acgatgttca ccgagtcggc ttacgatttc ccttgcggct
     1021 tcacgaccaa catcatcaac gtccatcgag atgaatattt cctcaaacct gtcgaggttg
     1081 tgatactcaa actcaatcca ctgttgctta gcgcctttcc cgccaccaaa cggcacggat
     1141 aacgccgaga tgccgtattg cgcatagctc atacaatcaa tttcgccttc gcaaagcaca
     1201 accaccctca cgccagcgtc cagagcctgc catccgaaca ggcaaggttc gcaatcacct
     1261 tctgccataa tgactttctt cccgtccggg cgctcagtgc tgattcgctt gacctgcaac
     1321 aactcaccat cgcgtttgta cggaagcacc agagcatcca gttctcgttc tccattccac
     1381 accttgccgc tgacaacctc gtagcgcttt acgacttctg gcgatatgcc acgcgattgc
     1441 aggtactcaa gatgggattc tgttctggta acgtagcggg cgattttctt acgatcaggt
     1501 ctggagaatt tcttctcacg tctggcatcg aaatggtgat cgtcatcctt gataccgaga
     1561 aatgctttcg cttcctgcat agcctgatgc aggttaattc cacgacatgc catccacaaa
     1621 tcaagcatgt caccgccgtc tccctcagcg aaatcagccc attttttctt gccgataagg
     1681 ttgaccttaa ggctgtttcc cttgtcaccg ttgacgttac cggcaaccca ctcatgcccc
     1741 tctatcttgc cgtttggcaa caggtgcgga gccaccctgt caacctgcgc ccaaagcagg
     1801 tcgctaagtt cacttggcct catgattccc tcagattgag atttttaaac cagaaatcga
     1861 caaacgaaat acttaaccag ccgtggttat aaccagcgac cagtagcgat ttgatttttg
     1921 atttcatggt tcacctgtcg aaaaacacat agccagtttt cgatacggtg attgcggatg
     1981 atggtctgga ttgtggttga atggtttctg gcttctcgtc gttccagcg
//
LOCUS       DAJTMX010000276         1990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTMX010000276 DAJTMX010000000
VERSION     DAJTMX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.276"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..319
                     /locus_tag="OO145_004265"
     CDS             <1..319
                     /locus_tag="OO145_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5469146.1"
                     /translation="DLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQ
                     DVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIR
                     CST"
     gene            complement(327..1760)
                     /gene="cscA"
                     /locus_tag="OO145_004266"
     CDS             complement(327..1760)
                     /gene="cscA"
                     /locus_tag="OO145_004266"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5469147.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
BASE COUNT          487 a          490 c          477 g          536 t
ORIGIN      
        1 cgatctcgca agtttactca atgctcactt caaatcgggc aaaccagatt ttgatgttct
       61 gatatgtggt aacgatcgcg cagcctttgt ggcttatcag gttcttttgg cgaagggggt
      121 acgtatcccg caggatgtcg ctgtaatggg ctttgataat ctggttggcg tcgggcatct
      181 gtttttaccg ccgctgacca caattcagct tccacatgac attatcgggc gggaagctgc
      241 attgcatatt attgaaggtc gtgaaggggg aagagtgacg cggatccctt gcccgctgtt
      301 gatccgttgt tccacctgat attatgttaa cccagtagcc agagtgctcc atgttgcagc
      361 acagccactc cgtgggaggc ataaagcgac agttcccgtt cttctggctg cggatagatt
      421 cgactactca tcaccgcttc cccgtcgtta ataaatactt ccacggatga tgtatcgata
      481 aatatcctta gggcgagcgt gtcacgctgc gggaggggaa tactacggta gccgtctaaa
      541 ttctcgtgtg ggtaataccg ccacaaaata agtcgctcag attggttatc aatatacagc
      601 cgcattccag tgccgagctg taatccgtaa tgttcggcat cactgttctt cagcgcccac
      661 tgcaactgaa tctcaactgc ttgcgcgttt tcctgcaaaa catatttatt gctgattctg
      721 cggggagaga cagattgatg ctgctggcgt aacgactcag cttcgtgaac cgggcgttgt
      781 agaagtttgc cattgctctc tgatagctcg cgcgccagcg tcatgcagcc tgcccatcct
      841 tcacgttttg agggcattgg cgattcccac atatccatcc agccgataac aatacgccga
      901 ccatccttcg ctaaaaagct ttgtggtgca taaaagtcat gcccgttatc aagttcagta
      961 aaatgcccgg attgtgcaaa aagtcgtcct ggcgaccaca ttccgggtat tacgccactt
     1021 tgaaagcgat ttcggtaact gtatccctcg gcattcattc cctgcgggga aaacatcaga
     1081 taatgctgat cgccaaggct gaaaaagtcc ggacattccc acatatagct ttcacccgca
     1141 tcagcgtggg ccagtacgcg atcgaaggcc cattcacgca atgaactgcc gcgataaagc
     1201 aggatctgcc ccgtgttgcc tggatctttc gccccgacta ccatccacca tgtgtcggct
     1261 tcacgccaca ctttaggatc gcggaagtgc atgattcctt ctggtggagt gaggatcaca
     1321 ccctgtttct cgaaatgaat accatcccga ctggtagcca gacattgtac ttcgcgaatt
     1381 gcatcgtcat tacctgcacc atcgagccag acgtgtccgg tgtagataag tgagaggaca
     1441 ccattgtcat cgacagcact acctgaaaaa cacccgtctt tgtcattatc gtctcctagc
     1501 gctagcgcaa taggctcatg ctgccagtgg atcatatcgt cactggtggc atgtccccag
     1561 tgcattggtc cccagtgttc gctcatcggg tgatgttgat aaaacgcgtg ataacgatcg
     1621 ttaaaccaga tcaggccgtt tggatcgttc atccatccgg caggaggcgc gaggtgaaaa
     1681 tggggataga aagtgttacc ccggtgctca tgaagttttg ctagtgcgtt ttgcgccgca
     1741 tgcaatcgag attgcgtcat tttaatcatc ctggttaagc aaatttggtg aattattaac
     1801 gttaactttt ataaaaatat agtcccttac tttcataaat gcgatgaata tcgcaaatgt
     1861 taacgttaac tatggcgttt tgtgatcgaa tatgcatgtt ttagtaaatc catgacgatt
     1921 ttgcgagaaa gaggtttatc actatgcgta actcagatga atttaaggga aaaaaatgtc
     1981 agccaaagta
//
LOCUS       DAJTMX010000277         1976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTMX010000277 DAJTMX010000000
VERSION     DAJTMX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.277"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..837)
                     /locus_tag="OO145_004267"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="OO145_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          533 a          465 c          457 g          521 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcag
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DAJTMX010000278         1964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTMX010000278 DAJTMX010000000
VERSION     DAJTMX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.278"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO145_004268"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO145_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5469148.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          332 c          311 g          699 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DAJTMX010000279         1951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTMX010000279 DAJTMX010000000
VERSION     DAJTMX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.279"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO145_004269"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO145_004269"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5469149.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>1951
                     /locus_tag="OO145_004270"
     CDS             1647..>1951
                     /locus_tag="OO145_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5469150.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          516 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa a
//
LOCUS       DAJTMX010000280         1929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTMX010000280 DAJTMX010000000
VERSION     DAJTMX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.280"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            274..578
                     /locus_tag="OO145_004271"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO145_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..>1929
                     /locus_tag="OO145_004272"
     CDS             747..>1929
                     /locus_tag="OO145_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5469151.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          508 a          380 c          439 g          602 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcat
//
LOCUS       DAJTMX010000281         1921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTMX010000281 DAJTMX010000000
VERSION     DAJTMX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.281"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(30..1181)
                     /locus_tag="OO145_004273"
                     /pseudo
     CDS             complement(30..1181)
                     /locus_tag="OO145_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1246..1894
                     /locus_tag="OO145_004274"
                     /pseudo
     CDS             1246..1894
                     /locus_tag="OO145_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          478 a          482 c          422 g          539 t
ORIGIN      
        1 gatcctaccc acgtaatgtg gacacggccc taagcgagat tatggttttc aaattgttcc
       61 gggctgagac cgccacaggc actgtgacga cgccagcgat tgtaatcaca ctcgatataa
      121 ttaaacaccg ttgcccgcat tatttcccgg ctgctaaagc gttccccgtg gatacattcc
      181 accttcagcg aatgaaagaa gctttccaca caggcattgt cataacagta gcctttcgca
      241 ctcatactgc cacgcaggtt gtgtcgcttc agcattgcct gataatcccc tgaacagtat
      301 tgaccaccac ggtccgtatg aacaatgacg ctttccgggc gtcttctccg ccacaacgcc
      361 atttgcagtg catcacaggc cagttgtgct gtcattcgcg gtgacatcga ccagccaatg
      421 acggcgcgtg accacaggtc gatgactact gcgagataca accagccctc atcggtacgc
      481 aagtacgtga tgtcacccgc ccacttctgg tttcgggcca ctggcgctga agtcctgctc
      541 cagcagattc tccaatacgg gcaggccatg tgcacggtag ctgaccgggc tgaacttccg
      601 gctggctttc gcccgcagcc cctgacgacg caggctggcg gcaatggttt taatattgaa
      661 ctccggcagt tcgtcagcaa ggcggggagc accgtatcgc tgctttgcct caaagaatgc
      721 cttatggaca gcggcatcgc aggtgagccg aaactgttgg cgcaggctca tctggtgacg
      781 acgcctgagc cagacatacc agccgctgcg ggcaacccga agtacacgac acatcgcttt
      841 gatgctgaac tctgcccgat gattttcgat aaagacatac ttcatttcag gcgcttcgcg
      901 aagtatgtcg cggccttttg gaggatggac agttcctcag cctgctccgc cagttgtcgt
      961 ttaaggcgga cattttcagc ggccagttcg ctttcgcgct ctgacgaact catttgttgc
     1021 tgctgtttac tgcgccaggc ataaagctga gattcataca ggctgagttc acgggctgcg
     1081 gcggccacac cgatgcgttc agcgagtttc agggcttcgt tacgaaattc aggcgtatgt
     1141 tgtttacggg gcttcttgct gattgatact ggttttgtca tgagtcacct ctggttgaga
     1201 gtttactcac ttagtcctgt gtccactatt ggtgggtaag atcaccctcc attaaaacta
     1261 aaacgggcgc tttctgatat atcatctgta agcgcagtaa taccattagt agcacctcgc
     1321 gcattgatta ttgaatatta taactggaat gttatatttc ttgttatgat gctgttcagt
     1381 attataataa tgttattatc accaaagata actcatgcaa atgagaaagt aatatgtcaa
     1441 aacagtgcat cctttgtgac caaagagttt atctgcggca ctatacttgt ctcattaata
     1501 ctcagttctc tattttgcta catatcaata tcaccatcaa ttttcatgca tacttatggc
     1561 ctgagtactt ttagttactc aattattttc tccttctcag ttttattttt cataatcgga
     1621 aatcaattat caaagattga aaaattatcc aacctatggt ttttattacc caatagccaa
     1681 ttttatcgca ttacaaataa atggttcaaa aacaggattt gccgcatcta ttacaggttt
     1741 tactcaaaca ataagcgctg gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg
     1801 tgttggtccc ggaactactt taggtcttgg ttcactaata acttcattac tgtcaatagc
     1861 cgtaatcgcc attgcaggaa ataataaaaa gtaagtgtac tctgactgcg tatgtatcta
     1921 c
//
LOCUS       DAJTMX010000282         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTMX010000282 DAJTMX010000000
VERSION     DAJTMX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.282"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(53..1702)
                     /locus_tag="OO145_004275"
     CDS             complement(53..1702)
                     /locus_tag="OO145_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5469152.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTMX010000283         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTMX010000283 DAJTMX010000000
VERSION     DAJTMX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.283"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(854..1327)
                     /locus_tag="OO145_004276"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="OO145_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="OO145_004277"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="OO145_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1913)
                     /locus_tag="OO145_004278"
                     /pseudo
     CDS             complement(1623..1913)
                     /locus_tag="OO145_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          536 c          459 g          493 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tga
//
LOCUS       DAJTMX010000284         1910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTMX010000284 DAJTMX010000000
VERSION     DAJTMX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.284"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            353..1711
                     /locus_tag="OO145_004279"
     CDS             353..1711
                     /locus_tag="OO145_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5469153.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          505 a          473 c          514 g          418 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa
//
LOCUS       DAJTMX010000285         1860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTMX010000285 DAJTMX010000000
VERSION     DAJTMX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.285"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..344)
                     /locus_tag="OO145_004280"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="OO145_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="OO145_004281"
     CDS             complement(344..1264)
                     /locus_tag="OO145_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5469154.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1860
                     /gene="pgrR"
                     /locus_tag="OO145_004282"
     CDS             1402..>1860
                     /gene="pgrR"
                     /locus_tag="OO145_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5469155.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          465 a          451 c          433 g          511 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtcctga ggtgtactgg caatagcgga
//
LOCUS       DAJTMX010000286         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTMX010000286 DAJTMX010000000
VERSION     DAJTMX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.286"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(208..609)
                     /locus_tag="OO145_004283"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO145_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO145_004284"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO145_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5469156.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTMX010000287         1824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTMX010000287 DAJTMX010000000
VERSION     DAJTMX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.287"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..172)
                     /locus_tag="OO145_004285"
     CDS             complement(<1..172)
                     /locus_tag="OO145_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469157.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPI"
     gene            complement(313..867)
                     /locus_tag="OO145_004286"
     CDS             complement(313..867)
                     /locus_tag="OO145_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5469158.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(864..1154)
                     /locus_tag="OO145_004287"
     CDS             complement(864..1154)
                     /locus_tag="OO145_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5469159.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1154..1753)
                     /locus_tag="OO145_004288"
     CDS             complement(1154..1753)
                     /locus_tag="OO145_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5469160.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          444 a          496 c          417 g          467 t
ORIGIN      
        1 tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact ccgtacattt
       61 ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca gggttggcgt
      121 aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc ataaatcgcg
      181 aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc gctccgtaaa
      241 catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata tagccccgga
      301 taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat cacgcatcgg
      361 tagatagagc atatattcag caagatttag ccatacatct acccgactgc agcacgtccg
      421 gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct tagtcatccc
      481 ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg ccagcacctc
      541 acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca gccaggtctt
      601 ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct ctattcccgc
      661 ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt tggatgccag
      721 caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc gcccccacat
      781 gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt gttccccgct
      841 tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat ttcttgtgtg
      901 cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc gctggttttt
      961 acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg catatcgtgt
     1021 ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac agaagtttcg
     1081 ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg cgccgcttta
     1141 cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg acttcctccg
     1201 gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca gatttataaa
     1261 cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc ctgcgcccac
     1321 gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt acccactcgc
     1381 ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc gcaactgtat
     1441 taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct actgattcgc
     1501 acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc gccccaccga
     1561 ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg tggaatgcgt
     1621 agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt tgatgcaaaa
     1681 aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt ttgaccagtt
     1741 gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag cccgttatgt
     1801 agtataccag aattaatgcc aata
//
LOCUS       DAJTMX010000288         1811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTMX010000288 DAJTMX010000000
VERSION     DAJTMX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.288"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..99
                     /locus_tag="OO145_004289"
     CDS             <1..99
                     /locus_tag="OO145_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5469161.1"
                     /translation="VFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(203..670)
                     /locus_tag="OO145_004290"
     CDS             complement(203..670)
                     /locus_tag="OO145_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5469162.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(736..975)
                     /locus_tag="OO145_004291"
     CDS             complement(736..975)
                     /locus_tag="OO145_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5469163.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1110..1715)
                     /locus_tag="OO145_004292"
     CDS             complement(1110..1715)
                     /locus_tag="OO145_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5469164.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          574 a          396 c          331 g          510 t
ORIGIN      
        1 gtgtttaatt atatcgaatg tgattacaat cggtggcggc ggcacagttg gtgtggcggc
       61 ctcagtccgg aacaatttga aaaccagaac cttgcttagg cctgtgtcca tattacgtgg
      121 gtaggatcac aagacctact gctatcacaa ttactgagag aaaaaaaagc atccgatgat
      181 tcatttatgc tgtcctaaaa aattaacaaa acaatgggaa aacaggatat gtaagcagaa
      241 aagccaatga aattggtaca gcataaggca caccgttttc acgaattgat tttcgaaaaa
      301 aaagaagacc tgccagaaca accagagcgc cgcaaaaggt cattgcgacg agaaaattag
      361 ccagttcatg ttcatcaata gctaacgaaa gaacagttaa cagcttgaca tcaccgccgc
      421 caattatccc tgcggaaaaa agaaagaagc ccacgagcaa aacgctcccg gctgcaacca
      481 gattgggttt ttgatgctgt aaaatgagtg ccaaaaaaat aagcacggtt aacaccagta
      541 aataacgatg gctaataatt ctgagtaata tatcccccca tgaaataaaa ataagaaaga
      601 gcgcaacaaa taaaagatcg actcttagta accatattgc tgccattgaa agactccatg
      661 taccatacaa ggttgctgcc agacatattc ctttacgtct gacagcctct agagagtttt
      721 tttatttttt ataagttacg gattactcgt tccggaaatc gttacctgtt gaattgctgc
      781 agcgatagcg tcgaatgcat ctttaattgc gcccaggaag cctgagttct gatcgccaaa
      841 gatgaaagca agcagagttg ccatagcaac accaatcaat gcatattcga ttgcagtaac
      901 accacgttca tccttcgcga aacgcgcaaa gcactgttta gttttaccat agtacttagt
      961 aataaaagta ttcatgtttt cctcgtatat tattatttaa attcaaacca ccagcttcat
     1021 ctgcgtgtca ccggtatccc atatttttgg gatgtggcta taattatcta ttcccgctat
     1081 ttagtcttca aaaaaaatta catttaatat catttatctt gattaagaaa tagtaagact
     1141 aaaaaaacaa gcgtcgtcag ccagcacact gctaacacgg taagaacgct aataaaaaca
     1201 ctccactgct tcgttctata ccattcaata tttttgtcag tatgttttat tgggagcagc
     1261 tcttcagtag agaattcctg gctgttatca ttctcaggaa tgtttgcatc agtgtttact
     1321 tccctggcct cgctatcgtt ggtatagcta ctactgtctt catcactgtc acgctcattc
     1381 tcacagacat gtttctcgac ctttcccaga tatctgacgc cttttcgcgg aatggtttta
     1441 attagagaac tcgacaaacc tgcgacgtct aatgcctttc taagcatgta aatctgccca
     1501 taataactgc tgtcgcttac cgaacctttc tgctcccgcc aaacctcatt gataatatgt
     1561 tgcttatcgg attctccctg tagtaaaagg agtaaaaatc gatagccgtt ttcagacaga
     1621 ttgattacat tgcctgtact ggtggtgata cgtcgagacg ctgggtaaaa cttaactccc
     1681 tgccctaaag aaaaataaaa acggtcatcg ctcataagta ccgacccctg ttttcttctt
     1741 cacatgacta ctcattacaa ctttgtatga agtaagatgc aaaatcatat tgtttacatt
     1801 aagttaagca a
//
LOCUS       DAJTMX010000289         1810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTMX010000289 DAJTMX010000000
VERSION     DAJTMX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.289"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..234
                     /locus_tag="OO145_004293"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO145_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO145_004294"
     CDS             655..1233
                     /locus_tag="OO145_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469165.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OO145_004295"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OO145_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1810
                     /locus_tag="OO145_004296"
                     /pseudo
     CDS             1584..1810
                     /locus_tag="OO145_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          588 a          322 c          423 g          477 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggaaggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc
//
LOCUS       DAJTMX010000290         1809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTMX010000290 DAJTMX010000000
VERSION     DAJTMX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.290"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            173..1198
                     /locus_tag="OO145_004297"
     CDS             173..1198
                     /locus_tag="OO145_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5469166.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="OO145_004298"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="OO145_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAJTMX010000291         1779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTMX010000291 DAJTMX010000000
VERSION     DAJTMX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.291"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..360
                     /locus_tag="OO145_004299"
     CDS             <1..360
                     /locus_tag="OO145_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5469167.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO145_004300"
     CDS             361..726
                     /locus_tag="OO145_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5469168.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO145_004301"
     CDS             723..1406
                     /locus_tag="OO145_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5469169.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="OO145_004302"
     tRNA            1605..1680
                     /locus_tag="OO145_004302"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="OO145_004303"
     tRNA            1682..1758
                     /locus_tag="OO145_004303"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          490 a          376 c          492 g          421 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtagg
//
LOCUS       DAJTMX010000292         1759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTMX010000292 DAJTMX010000000
VERSION     DAJTMX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.293"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..263)
                     /locus_tag="OO145_004304"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO145_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO145_004305"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO145_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5469170.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="OO145_004306"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="OO145_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAJTMX010000293         1746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTMX010000293 DAJTMX010000000
VERSION     DAJTMX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.294"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            453..1301
                     /locus_tag="OO145_004307"
     CDS             453..1301
                     /locus_tag="OO145_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5469171.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="OO145_004308"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="OO145_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          498 a          398 c          411 g          439 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagc
//
LOCUS       DAJTMX010000294         1688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTMX010000294 DAJTMX010000000
VERSION     DAJTMX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.295"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            126..995
                     /locus_tag="OO145_004309"
     CDS             126..995
                     /locus_tag="OO145_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5469172.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="OO145_004310"
     CDS             complement(1198..1551)
                     /locus_tag="OO145_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5469173.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
BASE COUNT          470 a          414 c          380 g          424 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaa
//
LOCUS       DAJTMX010000295         1679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTMX010000295 DAJTMX010000000
VERSION     DAJTMX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.296"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..800)
                     /locus_tag="OO145_004311"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="OO145_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(851..1426)
                     /locus_tag="OO145_004312"
                     /pseudo
     CDS             complement(851..1426)
                     /locus_tag="OO145_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1430..1679
                     /locus_tag="OO145_004313"
                     /pseudo
     CDS             1430..1679
                     /locus_tag="OO145_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          357 a          482 c          422 g          418 t
ORIGIN      
        1 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
       61 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
      121 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
      181 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
      241 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
      301 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
      361 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
      421 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
      481 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
      541 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
      601 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
      661 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
      721 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
      781 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
      841 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
      901 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
      961 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     1021 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     1081 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     1141 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     1201 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     1261 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     1321 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     1381 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     1441 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     1501 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     1561 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     1621 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggg
//
LOCUS       DAJTMX010000296         1669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTMX010000296 DAJTMX010000000
VERSION     DAJTMX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.297"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(4..537)
                     /locus_tag="OO145_004314"
     CDS             complement(4..537)
                     /locus_tag="OO145_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5469174.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(603..1232)
                     /locus_tag="OO145_004315"
     CDS             complement(603..1232)
                     /locus_tag="OO145_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469175.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1236..1451)
                     /locus_tag="OO145_004316"
     CDS             complement(1236..1451)
                     /locus_tag="OO145_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5469176.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          442 a          446 c          350 g          431 t
ORIGIN      
        1 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
       61 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      121 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
      181 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
      241 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
      301 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
      361 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
      421 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
      481 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
      541 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
      601 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
      661 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
      721 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
      781 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
      841 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
      901 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
      961 tcctctccgg cactgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1021 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1081 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1141 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     1201 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     1261 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     1321 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     1381 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     1441 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     1501 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     1561 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     1621 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaa
//
LOCUS       DAJTMX010000297         1644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTMX010000297 DAJTMX010000000
VERSION     DAJTMX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.298"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..430
                     /locus_tag="OO145_004317"
     CDS             <1..430
                     /locus_tag="OO145_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469177.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO145_004318"
     CDS             550..723
                     /locus_tag="OO145_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5469178.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="OO145_004319"
     CDS             823..1644
                     /locus_tag="OO145_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5469179.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          427 a          416 c          420 g          381 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctga
//
LOCUS       DAJTMX010000298         1634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTMX010000298 DAJTMX010000000
VERSION     DAJTMX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.299"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(27..1319)
                     /gene="yiaN"
                     /locus_tag="OO145_004320"
     CDS             complement(27..1319)
                     /gene="yiaN"
                     /locus_tag="OO145_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5469180.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWMVMLPTY"
     gene            complement(1322..>1634)
                     /locus_tag="OO145_004321"
     CDS             complement(1322..>1634)
                     /locus_tag="OO145_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5469181.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          502 a          415 c          411 g          306 t
ORIGIN      
        1 ccttaaaatc accatcatac actaaatcag taagttggca gcattaccat ccatttcaaa
       61 gggaggatga tgagatcggg aataaaaaca aatacgacta ataatgaata taaaaccagg
      121 acgtaaggga aaacgcctct gactgcatca tcgaatttga gttttgccac ccctgaaata
      181 acgttaagta cattaccgat aggcggtgta attaagccga ttgagcagtt aatgataaac
      241 atgacaccga aataaatcgg atcgattcct gcctctttaa ctaaaggcat taataccggg
      301 gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc cgacaatcag gatagccacc
      361 ataatgacga taaacagcag acgcggtgaa tcgaccaacg gttgcagtaa atcagaaacc
      421 atcatcggca gttcagcaat ggtaatcagc caggcggaaa cttgtgccga ggccaccaga
      481 aacataacca ctgaggtggt tttcgccgcg ccaatgagta catgccaaag tgtggcgaag
      541 gtcatttcac ggtaaataac tgtggcgaca aacagcgcat agaaagcagc aacggccccg
      601 gcttcggtag gggtaaacag accggagcga aacccgccaa taatgatgac tggaagaaac
      661 agcgcccaga taccggagac aaaagagtgc cagatttcct gcatcgttgc tttttgctgg
      721 cggggtaaat taaggcgact ggcctgccgc caccaggtaa gcatcagcgt tgcgcccatc
      781 attatgccag gggcaatgcc cgccataaac agcttactga tggataatcc gctggaaaca
      841 ccgaagataa taaacggaat ggaaggggga ataattggcg cgataatgcc gccagaggca
      901 atcagccccg ccgcccggtt gaccgggtag ttggcgctgc gcatcatcgg caccagtaga
      961 gcggcaacgg cggcggtatc cgcaacggca gaaccagaaa ggctcgccat aatcatagct
     1021 gccagcacgc cgacgtagcc cagcccgcct ggtttatgcc ccaccagttt catcggcagg
     1081 tcaacaatgc gttttaacaa gccgcccgca ttcatgattt cacccgccag cacaaagaac
     1141 ggaatcgcca ggagggagaa gctatcggct ccgttcacca gcgtttgtgc catgatctgg
     1201 acatcaaaca tgtccagcca gaacattaac gccgccccgc acaacaacag tgcccaggca
     1261 ataggcaaac cgatagcaat accacccaac agacagccca gaaaaatcag cacagccatg
     1321 attaagctcc ttgcggtggc gacgttagag aattgctacg cgtgatgagt tgatataaat
     1381 gacgcagttc aaagaatgcg ataacgaggc tggtgggcag acaagcggca tacatcaggc
     1441 cgatgggcaa accgaggatc ggtgaataat cgctccagtc ctgaattgtt tttagcgttg
     1501 cgccccaggc cagtgcgcca caaataaata agattaagga atgggtaacc agagcgactc
     1561 gtcgttgcca tgcgggggag agtttctcca ccagaaaggt gacctgaacg tgggcgttat
     1621 ccataaaagc caca
//
LOCUS       DAJTMX010000299         1624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTMX010000299 DAJTMX010000000
VERSION     DAJTMX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.300"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..352
                     /locus_tag="OO145_004322"
     CDS             <1..352
                     /locus_tag="OO145_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5469182.1"
                     /translation="GAFGDVIRQGETGQVNQLLDILRHKALTQMAQESGGSATVRLNT
                     LDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERL
                     SESMVDEIFQKGAA"
     gene            349..1419
                     /locus_tag="OO145_004323"
     CDS             349..1419
                     /locus_tag="OO145_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5469183.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT          385 a          370 c          483 g          386 t
ORIGIN      
        1 gggcgcgttt ggtgatgtga tacgtcaggg cgaaacaggc caggttaacc agctactgga
       61 tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta gtgcaaccgt
      121 gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag ataacgagtg
      181 gcatgacgcg attaactggc tgggggactg gtgcagtgaa gagcagcatc cggtgatctg
      241 gtcaaccacg caggctgcag aacacttacc ggttcgcatg ccccgtctct gctctgcaga
      301 acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat gagtatagaa
      361 ataaaaacag ggctggtgct gtccggcggt ggggcggtgg gcgcttatca ggcgggagtg
      421 gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcaggggc cagcattggc
      481 gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc cgtacgcctg
      541 gaggcgctct gggatcatct tgggaataat caggtgctgt cggtaaaccg atcggtttac
      601 ttttcattgc tgaaaaaatt gttccaggca atgaacctct gccagatccc cggacgtgca
      661 ggagcactgc ttacgacact tcttcgccat atatcgataa tcaacggctt cgacaatccg
      721 atgactcagc cgttgttgtc agatgggccc ctgacagcgt tgatggatca ttatcttgat
      781 acagatgctc tggcaggcgg gctaccgctg tatgtgtcgc tgtaccccac agaagggggc
      841 atgcaggata ttattgactg cattcgtgct gaactgggtt ccggaaccac gaaaaacgcc
      901 gtttttcagc atatccagag tctgccccgc ggacagcaga aagaggctct gcttgcgtca
      961 gccgcactgc ccctgttgtt ccgtccccgt gaggttcagg ggacaatgtt cggtgatggt
     1021 ggtatgggag gatggcgaaa tatgcaggga aatacccctg taacgcctct ggtcgatgcc
     1081 ggatgcaata tggtgattgt gtcgcatctg agtgacggtt ctttatggga tcgccaggct
     1141 tttccggaca ccacaatcct tgagatccgt ccccggaaaa ggctgaaata tgcaggtgat
     1201 ggtggcaaca gtggcggtct gctcagtttc acgtcggcac ataccgacgc ctggcgtcag
     1261 cagggctacg aggacacgat gctgacgatg gagcatatcc ggaaaccgct ggcagcacgt
     1321 caggcactgt cccggtcaga aacggtgttg cagaaaagtc tggagataac ggaagaggca
     1381 gatttggcac tgagaagcgc gatggcccgg attaaataaa gacgccccaa agaaacgcca
     1441 ccgcctgtgg tggtgttttt cctgtctggt gttgttgaaa atattttcag atatatatta
     1501 cctgggtgag atttaagatt aacgcattta ttaatgtcat aaggagagtt atcatgaaat
     1561 tcatcgtaaa agcacttagg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     1621 ttga
//
LOCUS       DAJTMX010000300         1604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTMX010000300 DAJTMX010000000
VERSION     DAJTMX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.301"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            240..509
                     /locus_tag="OO145_004324"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO145_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO145_004325"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO145_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO145_004326"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO145_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5469184.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          477 a          330 c          377 g          420 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaa
//
LOCUS       DAJTMX010000301         1601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTMX010000301 DAJTMX010000000
VERSION     DAJTMX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.302"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(333..479)
                     /locus_tag="OO145_004327"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="OO145_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="OO145_004328"
     CDS             533..715
                     /locus_tag="OO145_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5469185.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="OO145_004329"
     CDS             complement(816..1145)
                     /locus_tag="OO145_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5469186.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="OO145_004330"
     CDS             complement(1317..>1601)
                     /locus_tag="OO145_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469187.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DAJTMX010000302         1586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTMX010000302 DAJTMX010000000
VERSION     DAJTMX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.305"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..691)
                     /locus_tag="OO145_004331"
                     /pseudo
     CDS             complement(1..691)
                     /locus_tag="OO145_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(739..1311)
                     /locus_tag="OO145_004332"
     CDS             complement(739..1311)
                     /locus_tag="OO145_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5469188.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          469 a          333 c          318 g          466 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttacgc gtactaccaa tgacaacgcc tgcataagtt aatgctggtt
      601 ggcctaaaaa aacgctgagt ataatcgcgg taaataactt acattaaact gttgttttaa
      661 agagcctgat taaacaactt tttctggaca taacgactcc ctggccaaag ccaactgaat
      721 aaaaaggccc ttgataaatt atttcttata ttcgacagta aattgtgaat cagcgttcgc
      781 cgtaccagcc gttacggtcg cattggttgc cacatagcgt gcaataaact tcatttcagc
      841 cttgccttca gcatcgatag cggtataagc agagtcgtta taaagtttaa cctgcgaatt
      901 atcggactgg ttaaaaatac gaataccaac gccggttgcc gcaatcgctt gtcctgtacc
      961 ggtaaaatca ccagggttgc ctgctgtaag cggcgtaccc actttcaggt cgccgtcgcc
     1021 gccaggcgct tctgtaccat caaaacgaac ggctgcttga gtgtacgttg ccgggcaatt
     1081 ttcaagttta atactgaact tctgtggcga cgccagacta cctacacctg aaaatgcatt
     1141 tttactgact ttacccagtg ggacaatttg atcggctgta tcggaggtaa cttcacaagt
     1201 agtgtcgaca atttcaccga cgaagttaac aacaccatct tctgccagtg catgcccggc
     1261 cagcattaga gatgccagag tgaatgcacc gcctataata ttacgcttca taaaaactcc
     1321 attgatataa tatatccttt accatgaatc atcacttata ttaataagtg tgtttttgat
     1381 tcaaaggaat agtacaccaa agagacggct ttatctaaaa ataatattaa ttacgcaaaa
     1441 ataaaatata gatttattgt ttaaatatta tttaaacagt aaaaaatcaa gtttgttatc
     1501 agacattaat gataattcag aatatattta tatagaaaga tagatgaatt tattgatgtc
     1561 agttataaaa aagggccacc ggtcgg
//
LOCUS       DAJTMX010000303         1437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTMX010000303 DAJTMX010000000
VERSION     DAJTMX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.306"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..373
                     /locus_tag="OO145_004333"
     CDS             <1..373
                     /locus_tag="OO145_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469189.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="OO145_004334"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="OO145_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5469190.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..>1437
                     /locus_tag="OO145_004335"
     CDS             740..>1437
                     /locus_tag="OO145_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469191.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          340 a          400 c          398 g          299 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 tgcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgc
//
LOCUS       DAJTMX010000304         1416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTMX010000304 DAJTMX010000000
VERSION     DAJTMX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.307"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..585)
                     /locus_tag="OO145_004336"
     CDS             complement(<1..585)
                     /locus_tag="OO145_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5469192.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVR"
     gene            complement(582..944)
                     /locus_tag="OO145_004337"
     CDS             complement(582..944)
                     /locus_tag="OO145_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5469193.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(1206..>1416)
                     /locus_tag="OO145_004338"
     CDS             complement(1206..>1416)
                     /locus_tag="OO145_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5469194.1"
                     /translation="TWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHAS
                     KIDAIISRNGTNTAQEENVVYLNAR"
BASE COUNT          401 a          326 c          293 g          396 t
ORIGIN      
        1 gcgtacatat tcaacgacat ccgtcggacc acccacccag ctcagtatgc ctttcatgaa
       61 aagattgcgc tcaggcaaca gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa
      121 atctccgaca ttttcctcga tctttggggt gcttattttg ttgtgtaatt tatagaacca
      181 ctcagctgtc ttacgtttca gtcgtccatc agttgagcgg tctgaacgtt tagcaagcac
      241 catgtcagca cctgcctgcc acttttcaat aagatgaggg ataacctcaa ttgggtcttg
      301 caggtcgaca tcaataggaa ttacagcatc gccgcttgca tggtctaacc ctgcaaataa
      361 ggctggttct ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac
      421 agccagcgcg ttaataattg actctgtggc atctttactt ccgtcattta tgaatacaat
      481 ttctacttca tatggcttca actcttggaa ttcacgtacc gttttataga aaacaggtat
      541 cgcttcttct tcattgaaga caggaacgac aagagatatt ttcatttcgc atccctaaag
      601 acaatgaact ttgaatagac gaaaccgcac accagactga tggcggagaa ggtgataaga
      661 gtgactattg gaggaagtga gcatttatca gctgcccaac caacaatcac actcaatatt
      721 cccataaatc ccacgtataa catgtagcgc atcgctgtag tcaatgcttt gaatgtgaat
      781 cttgcattcg cgaagaagct aaagctcaca gccactacga aacctgtgaa gtttgccaga
      841 gcctgactgg tatgtgcggc atagatacat acaccaaaaa ccacccagtg tataagtgtg
      901 ttcagcacac ctatagatgt gtactttaca aataacttta acatttattt aatcaatgag
      961 ctctgaaagg catgaagtct atcatccaag tctcaattga tcgatacttg ctatgtctga
     1021 tgagacaaaa ctgagacaca taaggcctca caatggcttg caaggcttta catgttttga
     1081 tgtggtggga cgtgtgagcg cagtgttgat gggataatcc tttgaattac aagcggattc
     1141 ttataattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc atttaaatca
     1201 ataagttacc tcgcatttaa gtaaactacg ttctcctctt gtgccgtatt tgtgccattg
     1261 cgacttataa tcgcatcgat tttgctcgcg tgctcggtaa gatgcccggc tgaaaggtgg
     1321 gcgtatcttt gaaccatttc gagagtttcc catcctccca tctctttaag cgcaagaaga
     1381 gagacaccgg actgaaccag ccagcttgcc caggta
//
LOCUS       DAJTMX010000305         1411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTMX010000305 DAJTMX010000000
VERSION     DAJTMX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.308"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            31..291
                     /locus_tag="OO145_004339"
     CDS             31..291
                     /locus_tag="OO145_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5469195.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            297..1394
                     /locus_tag="OO145_004340"
     CDS             297..1394
                     /locus_tag="OO145_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5469196.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          437 a          319 c          346 g          309 t
ORIGIN      
        1 gcttatccga tttttgatcg tattgaagaa atggcatggg ctcgtcacta ccagcagatc
       61 gttcgtgaag aaaaagaaac ggaactggcg gacgacctgg aaaaaggtct gcctcagcac
      121 ctgtttgaat cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc
      181 agccacgcat ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac
      241 atggctgaaa ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg
      301 cgctttttca acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg
      361 ctggcgacgg caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc
      421 ggcaacgggg aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct
      481 ctgactgggt taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc
      541 gctcgtttgt tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga
      601 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
      661 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      721 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      781 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      841 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      901 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      961 gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt
     1021 ctcttaacct gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat
     1081 ttgccaacga cggtacaccg acatggctaa aagaaaagcg tgcaaaagaa atcgcacttg
     1141 atgaaattat cgagttgctc aacaagcacc atgggggcac cagtaacgac gccaaacgcg
     1201 caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga atcaaaggta
     1261 tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg gaaggcgttg
     1321 aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga tacgatgaga
     1381 atattccggt gtaaaaccaa gtgcattttg g
//
LOCUS       DAJTMX010000306         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTMX010000306 DAJTMX010000000
VERSION     DAJTMX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.309"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..167
                     /locus_tag="OO145_004341"
     CDS             <1..167
                     /locus_tag="OO145_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5469197.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="OO145_004342"
     CDS             152..790
                     /locus_tag="OO145_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5469198.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="OO145_004343"
     CDS             1018..>1404
                     /locus_tag="OO145_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469199.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          408 a          256 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggaggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAJTMX010000307         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTMX010000307 DAJTMX010000000
VERSION     DAJTMX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.310"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            400..1149
                     /locus_tag="OO145_004344"
     CDS             400..1149
                     /locus_tag="OO145_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5469200.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTMX010000308         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTMX010000308 DAJTMX010000000
VERSION     DAJTMX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.311"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(3..485)
                     /locus_tag="OO145_004345"
     CDS             complement(3..485)
                     /locus_tag="OO145_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5469201.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO145_004346"
     CDS             complement(476..778)
                     /locus_tag="OO145_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5469202.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="OO145_004347"
     CDS             1050..1322
                     /locus_tag="OO145_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469203.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAJTMX010000309         1345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTMX010000309 DAJTMX010000000
VERSION     DAJTMX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.312"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(196..885)
                     /locus_tag="OO145_004348"
     CDS             complement(196..885)
                     /locus_tag="OO145_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5469204.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(882..1241)
                     /locus_tag="OO145_004349"
     CDS             complement(882..1241)
                     /locus_tag="OO145_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5469205.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
BASE COUNT          328 a          381 c          268 g          368 t
ORIGIN      
        1 accactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
       61 catctgacca ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc
      121 ggcggcgggt tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca
      181 tgagatatat gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg
      241 ttctgaacgt gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg
      301 tttcattaca acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc
      361 cagtaatgcc agctcctgat attcataacc ttccccacca aaaagttctg cttttaccgc
      421 ctgcgccgcc agccagatta attttttcag gcgttcctgt gttttctctg caattttcct
      481 agtgccagaa tgagccttaa actcttccca cgcccagtgg gttatggtga tctgatactc
      541 ccagcaaata cttccgctgt aacaccacaa caaccaggct ttatagtgct cttcaagaga
      601 cagaacagcc cgccgccacg atgatgtcga aaacgcaacc tgactgacca gaggaattga
      661 cgcccccttc gccagtgatt gttttcccgg gattggtgga ttatcccgcg ttatcatttt
      721 tccggtcacc tcatcgcggt accggatttt tttacgcctg taacgccctg tatcgaacat
      781 ggcattctcc tgccaggctt caagctgacc ttttgtcgcc ccactcaaat cagcggtggc
      841 gataatgagc tgctcacgaa caaactgtaa atactggtta ttcatgcaca ctccagctct
      901 gtgattttta tccccaaccg accaccagga acaggcagtc cgcgcacaat attggtttca
      961 tcaaactgct cgtcgtcgat aagcaacccc gcatgtgtca gtgcatccag tggtgctttc
     1021 aggatattgt ccaggtcacg acgccgctta tccggtggct ctgcaataat ctttatcgcc
     1081 agtcttccgg acaggcttaa tttcagtcgc tgctgacgga caataagcgc cacatcccgg
     1141 cgataacgct caccggcttt tgatacaaaa tatgtgctgc cacgacgtcg ccagtaagta
     1201 ttcaccgtcg gcggataagg taaaataaac tcatggcgca tcagcgcagc acctcctgca
     1261 ccagcttttc aaactttccg actctggttt ccagctctgc cacacagtcc accagctcat
     1321 ctactgcttt ctgtgcgcga tgctt
//
LOCUS       DAJTMX010000310         1335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTMX010000310 DAJTMX010000000
VERSION     DAJTMX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.313"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..732
                     /gene="pcnB"
                     /locus_tag="OO145_004350"
     CDS             <1..732
                     /gene="pcnB"
                     /locus_tag="OO145_004350"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706091.4"
                     /note="Polymerase that creates the 3' poly(A) tail found
                     in some mRNA's; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HCR5469206.1"
                     /translation="ISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLL
                     CEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWY
                     PLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSR
                     RQGKRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNE
                     LDEEPSPRRRTRRPRKRAPRREGTT"
     gene            729..1208
                     /gene="folK"
                     /locus_tag="OO145_004351"
     CDS             729..1208
                     /gene="folK"
                     /locus_tag="OO145_004351"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5469207.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
BASE COUNT          337 a          355 c          328 g          315 t
ORIGIN      
        1 atcagcccgg aaaccgcaga accgatccct cgcctcgcta ccctgctgaa cgatatccca
       61 ccggcacgcc tgtttgaaga atcgcttaaa ctgctacaag cgggctacgg ttacgaaacc
      121 tataagctgt tgtgtgaata tcatctgttc cagccgctgt tcccgaccat tacccgctac
      181 ttcacggaaa atggcgacag cccgatggag cggatcattg aacaggtgct gaagaatacc
      241 gatacgcgta tccataacga tatgcgcgtg aacccggcgt tcttgtttgc cgccatgttc
      301 tggtacccac tgctggagac ggcacagaag atcgcccagg aaagcggcct gacctatcac
      361 gacgctttcg cgctggcgat gaacgacgtg ctggacgaag cctgccgttc actggcaatc
      421 ccgaaacgtc tgacgacgtt aacccgcgat atctggcagt tgcagttgcg tatgtcccgt
      481 cgtcagggta aacgcgcatg gaaactgctg gagcatccta agttccgtgc ggcttatgac
      541 ctgttggcct tgcgagctga agttgaacgt aatgctgaac tgcagcgtct ggtgaaatgg
      601 tggggtgagt tccaggtttc cgcaccacca gatcaaaaag ggatgcttaa cgagctggat
      661 gaagaaccgt caccgcgtcg tcgtactcgt cgtccacgca aacgcgcacc gcgtcgtgag
      721 ggtaccacat gacagtggcg tatattgcca taggcagcaa tctggcctct ccgctggagc
      781 aggtcaatgc tgccctgaaa gcattaggcg atatccctga aagccgcatt cttgccgttt
      841 cttcgtttta ccgcacccca ccgctggggc cgcaagatca acccgattac ctcaacgccg
      901 ccgtggcgct tgaaacttcg cccgccccgg aagagttact caaccataca cagcgcatag
      961 aactgcaaca gggtcgtgtt cgtaaagctg aacgctgggg accacgcacg ctggatctcg
     1021 atatcatgct gtttggtaat gaagtgataa ataccgaacg cctgaccgtt ccgcactacg
     1081 atatgaagaa tcgtggattt atgctgtggc cgctgtttga gattgcaccg gagctggcgt
     1141 ttcctgatgg ggagacgttg cgtgaagtct tacatacaag agcatttgac aaattaagca
     1201 aatggtaatt tatttatatt acgaatataa atattttctg aaaatcatac atcctctctt
     1261 tccttaataa aaattttagt ttaaacattt ttgcagaaac ataatataaa ttcccgcaga
     1321 taaatatcaa tccca
//
LOCUS       DAJTMX010000311         1330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTMX010000311 DAJTMX010000000
VERSION     DAJTMX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.314"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(41..100)
                     /locus_tag="OO145_004352"
     CDS             complement(41..100)
                     /locus_tag="OO145_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5469208.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="OO145_004353"
     CDS             complement(339..1247)
                     /locus_tag="OO145_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469209.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAJTMX010000312         1284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTMX010000312 DAJTMX010000000
VERSION     DAJTMX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.315"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            522..806
                     /locus_tag="OO145_004354"
     CDS             522..806
                     /locus_tag="OO145_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5469210.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="OO145_004355"
     CDS             806..1081
                     /locus_tag="OO145_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5469211.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          338 a          321 c          325 g          300 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag gacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc caga
//
LOCUS       DAJTMX010000313         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTMX010000313 DAJTMX010000000
VERSION     DAJTMX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.316"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO145_004356"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO145_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5469212.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTMX010000314         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTMX010000314 DAJTMX010000000
VERSION     DAJTMX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.317"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            34..435
                     /locus_tag="OO145_004357"
     CDS             34..435
                     /locus_tag="OO145_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5469213.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO145_004358"
     CDS             447..818
                     /locus_tag="OO145_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5469214.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO145_004359"
     CDS             805..>1275
                     /locus_tag="OO145_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5469215.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTMX010000315         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTMX010000315 DAJTMX010000000
VERSION     DAJTMX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.318"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO145_004360"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO145_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5469216.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          367 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DAJTMX010000316         1159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTMX010000316 DAJTMX010000000
VERSION     DAJTMX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.320"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="OO145_004361"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="OO145_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR5469217.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAJTMX010000317         1142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTMX010000317 DAJTMX010000000
VERSION     DAJTMX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.321"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..732
                     /locus_tag="OO145_004362"
     CDS             <1..732
                     /locus_tag="OO145_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5469218.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(734..960)
                     /locus_tag="OO145_004363"
                     /pseudo
     CDS             complement(734..960)
                     /locus_tag="OO145_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          300 a          284 c          247 g          311 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg gcatctgtta ctcacatcgc gtctttgttg cgttgaatga aaagcctaat
      361 gtaacagcaa tactttgtgg tggtacgtat cgtgccagaa gtgatgcttt ttacgatgcc
      421 agcaactctt cgccattaga ctctctcaat ccgcgaaaaa tatttatttc cgccagcggt
      481 gtgcataatc actttggcgt cagctggttt aaccctgaag atcttgccac taagcgtaaa
      541 gtgatgaacc gtggactacg gaaaattttg ctcgcccgcc acgcgttgtt cgatgaagtg
      601 gcctctgcca gcctcgcacc gatctctgca tttgacgttc tgattagcga tcgtccgtta
      661 ccggcagatt atgttacgca ctgccagaat ggttctgtaa agatcattac acctgattca
      721 gaagacgaat gacttactga aaaaacacca caatcttgtt aaacatcgtc gggttggact
      781 gattacgttg cactttcacc acatattcca gcttatctat ttggcttatc acctactcca
      841 gacgctggtc atccttgacc agtagccaga tatggctttt gtcgcagtcc tgaatcggca
      901 ggcagagaat gtcttcaacg ttaagagcgc gacgggcaaa agcccacaaa ggtgagtcat
      961 tacacccgga tggtaaacag gcacttcctt gttgtagaag ccgttatatt gtccataatg
     1021 tctgggtttt taatgcatac tgggtaacaa ccggacgaat cttcgaatat cactggcgct
     1081 gccagccggt ttgaattcat ctcacaaccc tgcatagagc gaatctcctg cctgcacgtc
     1141 ac
//
LOCUS       DAJTMX010000318         1110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTMX010000318 DAJTMX010000000
VERSION     DAJTMX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.322"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..1019
                     /locus_tag="OO145_004364"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO145_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAJTMX010000319         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTMX010000319 DAJTMX010000000
VERSION     DAJTMX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.323"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            55..>1100
                     /locus_tag="OO145_004365"
     CDS             55..>1100
                     /locus_tag="OO145_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5469219.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          345 a          255 c          177 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtatccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTMX010000320         1091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTMX010000320 DAJTMX010000000
VERSION     DAJTMX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.324"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO145_004366"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO145_004366"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5469220.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="OO145_004367"
     tRNA            complement(986..1062)
                     /locus_tag="OO145_004367"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAJTMX010000321         1074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTMX010000321 DAJTMX010000000
VERSION     DAJTMX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.325"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO145_004368"
     CDS             complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO145_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5469221.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          302 a          322 c          252 g          198 t
ORIGIN      
        1 gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata
       61 taaagccact ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc
      121 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa
      181 ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc
      241 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg
      301 taatgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt
      361 tccagcagag tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
      421 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
      481 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      541 agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc
      601 actaacagga agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa
      661 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      721 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
      781 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
      841 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc
      901 agaacggtca aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      961 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     1021 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg tgaa
//
LOCUS       DAJTMX010000322         1070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTMX010000322 DAJTMX010000000
VERSION     DAJTMX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.326"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            20..562
                     /locus_tag="OO145_004369"
     CDS             20..562
                     /locus_tag="OO145_004369"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469222.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            789..>1070
                     /locus_tag="OO145_004370"
     CDS             789..>1070
                     /locus_tag="OO145_004370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5469223.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          315 a          202 c          257 g          296 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca
      661 ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt
      721 tagacagtga gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg
      781 atttgcatat ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga
      841 atgtaaacgg aggattttct ttctcaaact catggatagc agcagatgcc gatggcactg
      901 aacaatttac ggggatatat tttagaacgc cggcctcaac gcagtcacat aaaattgtca
      961 gtggtgttca tatcaacact gcaaacaaaa acacggctgc aaacaatcaa agtatagcga
     1021 tagaacagtc ggcgatcttc gtctttgtaa gtggttgtac gttaactggt
//
LOCUS       DAJTMX010000323         1066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTMX010000323 DAJTMX010000000
VERSION     DAJTMX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.327"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(226..651)
                     /locus_tag="OO145_004371"
     CDS             complement(226..651)
                     /locus_tag="OO145_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469224.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(648..1031)
                     /locus_tag="OO145_004372"
     CDS             complement(648..1031)
                     /locus_tag="OO145_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469225.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          252 a          306 c          265 g          243 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcgat aaaaacatcg ccatatcctc
      421 aatgtaaggg tgggacttct ccggattcag caccacacag tccgccttct gttgctcact
      481 cagggcatgt aaatcatgct caaaccacgc cccctgagca tctgtctgca gaatcaaccg
      541 gccatggcag gaaaggcaga aacactgccg gatatttctg ctgagcgcag agtcggcact
      601 gataatgtgc tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc
      661 ccctcctcac tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg
      721 tggtgacatt gcctgcagtg ccagctggct ttacgtacta cgggtaaggt atccgggacg
      781 aattgctgca gacgtttaat caaccgtatt tctctgcgtt ccggcctgac ataagggcac
      841 tcatgaccgt gctccgtcag cccttcgtca gcgtgttcaa accagggccg ttcagtgtcg
      901 tattgcgggt ggtatctgag tgcactgccg caaaggtggc aggtgtagct atcgtaaggt
      961 gcagtctgtg cggtacgggc accggtcaga cgtccgttgc catcaagtgc gagaaaagat
     1021 tttgcgtaca tagtatatgt tcctcagccg ccagacgaca cgcagg
//
LOCUS       DAJTMX010000324         1066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTMX010000324 DAJTMX010000000
VERSION     DAJTMX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.328"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(570..749)
                     /locus_tag="OO145_004373"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="OO145_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="OO145_004374"
     CDS             843..>1066
                     /locus_tag="OO145_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469226.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAJTMX010000325         1050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTMX010000325 DAJTMX010000000
VERSION     DAJTMX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.329"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            62..580
                     /locus_tag="OO145_004375"
                     /pseudo
     CDS             62..580
                     /locus_tag="OO145_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          322 a          234 c          220 g          274 t
ORIGIN      
        1 cagaacttta cgcccacagc acctaatcag gtctgggtcg cagattcagt tgtccaggcc
       61 ttccgaaatc aaccgactga aggcgctggt cgcgaaactg cagcgtatgc agttcggtaa
      121 aagctcagaa aaacttcgcg caaaaaccga acggtggata caggaagcac aggagcgaat
      181 cagcgcactt caggaggaaa tggcggaaac actgggtgag caatatgacc cggtactgcc
      241 atcttccctg cgccagtctt cagcccgtaa accgttacca gcctcacttc cccgtgcccc
      301 ccgggtcatc cggccggaag aggaatgctg tccggcctgt ggtggtgaac tcagtccact
      361 gggatgtgat gtgtcagagc agctggagct tatcagcagc gcctttaagg ttatcgaaaa
      421 acaacgccct aaactggcct gtcgccggtg cgaccatatc gtgcaggcac cggtgccttc
      481 aaaacccatt gcacgcagtt atgccggagc ggggcttctg gcccatgttg ttaccggaaa
      541 gtatgcagac catctgccat tataccgcca aagtgatcta ctattccaca cagctattta
      601 gctctctttt tgttgagctc ccatgattct ttcatttatt gttactaatt attttatgtt
      661 atcatgatgt tagaacacgc tctgctctgt gtctctattt tatctatcat attctgagga
      721 ttaactacat aacaaggcgc aagatatata tacaaaaaca aataataccg ttgcaataat
      781 tccttttagt aaaggaggtg tgattaattt caggggattt cagagtaaaa attgtacaac
      841 atattaaaat atgttcattt cgttataata cacacaagga aatattaagt tagataatat
      901 attctaaata accatagata aaaacgcaca taatggggaa tattttatga aaatacctga
      961 agcagtaaat catattaatg ttcagaataa tattgatctt gttgatggaa aaacgaatcc
     1021 gaataaagca acaaaagcat tacaaaaaat
//
LOCUS       DAJTMX010000326         1020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTMX010000326 DAJTMX010000000
VERSION     DAJTMX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.330"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(50..190)
                     /locus_tag="OO145_004376"
     CDS             complement(50..190)
                     /locus_tag="OO145_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469227.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="OO145_004377"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="OO145_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5469228.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          205 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
//
LOCUS       DAJTMX010000327          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTMX010000327 DAJTMX010000000
VERSION     DAJTMX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.332"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            82..>990
                     /locus_tag="OO145_004378"
     CDS             82..>990
                     /locus_tag="OO145_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5469229.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAJTMX010000328          977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTMX010000328 DAJTMX010000000
VERSION     DAJTMX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.333"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..200
                     /locus_tag="OO145_004379"
                     /pseudo
     CDS             1..200
                     /locus_tag="OO145_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..926
                     /locus_tag="OO145_004380"
                     /pseudo
     CDS             300..926
                     /locus_tag="OO145_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT          305 a          228 c          218 g          226 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aaggctgagg tgtactggca atagcggaca ctaccatttg
      961 ttcttttttt aagcagc
//
LOCUS       DAJTMX010000329          956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTMX010000329 DAJTMX010000000
VERSION     DAJTMX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.334"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(55..243)
                     /locus_tag="OO145_004381"
     CDS             complement(55..243)
                     /locus_tag="OO145_004381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469230.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          249 a          189 c          224 g          294 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aagttgaacc gccccgggaa tcctgg
//
LOCUS       DAJTMX010000330          951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTMX010000330 DAJTMX010000000
VERSION     DAJTMX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.335"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            96..>951
                     /locus_tag="OO145_004382"
     CDS             96..>951
                     /locus_tag="OO145_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5469231.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKRE"
BASE COUNT          210 a          250 c          281 g          210 t
ORIGIN      
        1 tttgcgacca ttcttgccgg tatcagcgcc ggcggtatgt actgcccgga gtgtggcacg
       61 gttcactggc cggacggagt cgccccgccc ttctgatgct cccccgtttt gccgatattt
      121 ttcagcaggg aaaccgctgg cttaactggc tggagaaaca accggaaggg gcagtccgtc
      181 cggtggtaat tgaatcagtg acaaaaatca tggcctgcgg gaccacactg atgggctaca
      241 cgcagtggtg ttgttcatca ccggactgtt gccatacaaa aaaggtctgc ttccggtgta
      301 aaagccgctc ctgcccgcac tgcggggtga aggctggcgc acagtggata cagtatctgc
      361 tgagcctggt cccctactgc ccgtggcagc atattgtgtt cacacttccc tgccagtact
      421 ggtccctggt gttccacaac cggtggttac tggcagagat gagccgcatt gcagcggatg
      481 tgatactgga aatctgccat caggcagatg tggagccggg gatattcacg gtgatccaca
      541 catgggggcg tgaccagcag tggcatccgc atatccattt atcgacaact gccggtggtg
      601 tgacgtcggg ccacacctgg aaaaatcttc atttttacgc ccgtaaggtg atgagcatgt
      661 ggcgctaccg gataacacgg ttgctgtcac ggaaataccc tgacctggtg atgccggatg
      721 cgctggcagc agaaggaagc agtaaacggg aatggaatcg cttcctggac acgcattacc
      781 gccgtggctg gaatgtcaac gtatcccggg taatggataa tgccacccat gtggcggtgt
      841 actttggctc ttacctgaaa aagccaccgg tgccgatgag tcgtctggag cattatgccg
      901 gtcaggatga aatcggtctg cgttacaaca gtcaccgaac aaaacgggaa g
//
LOCUS       DAJTMX010000331          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTMX010000331 DAJTMX010000000
VERSION     DAJTMX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.336"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(201..866)
                     /locus_tag="OO145_004383"
     CDS             complement(201..866)
                     /locus_tag="OO145_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5469232.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          245 a          232 c          215 g          255 t
ORIGIN      
        1 gtaagaggct aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc
       61 ttgccggacg cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct
      121 gaagcagata gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat
      181 cagaaaggtg ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt
      241 ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca
      301 cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac
      361 gagccgccct gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg
      421 gctcgccgcc cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa
      481 ctgctgatca cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg
      541 tcaaaacaca tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt
      601 tcaaaccagt tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca
      661 aagcgaatat tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg
      721 tagaaaaaga tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa
      781 catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg
      841 ggccgtttga aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag
      901 agtgtagcat tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTMX010000332          936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTMX010000332 DAJTMX010000000
VERSION     DAJTMX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.337"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            144..641
                     /locus_tag="OO145_004384"
                     /pseudo
     CDS             144..641
                     /locus_tag="OO145_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="OO145_004385"
                     /pseudo
     CDS             682..873
                     /locus_tag="OO145_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAJTMX010000333          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTMX010000333 DAJTMX010000000
VERSION     DAJTMX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.338"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            377..>933
                     /locus_tag="OO145_004386"
     CDS             377..>933
                     /locus_tag="OO145_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5469233.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          334 a          181 c          150 g          268 t
ORIGIN      
        1 ctcgtttaag ctccattttc aaaaaaaata tatcagggtt tgttactaat aacttctgaa
       61 aacagaaaaa aatagagcag taattctatt tcagagagga gtaactccat ctatcctcga
      121 tacgtactga acgttatcgt gatatattca tcatgctttc ttcaccatta ttcgagtata
      181 gggagagacg caatgacctt aaataccttt ttatggctga attaccattc tcaggaataa
      241 cagcagactc cttcccatag acggcttagg gggaaagcaa tacccggaga aagggtacaa
      301 agcaaactgt tttaaaatta ataagccata ttctatttaa ctagaattct tatcgttgat
      361 aagaattcta gttataatga ttaaatcaac caatatacag gcaatcggtt ctggcattat
      421 gcatcaaata aacaatgtat actcgttaac tccattatct ttacctatgg aactgactcc
      481 atcttgtaat gaattttatt taaaaacctg gagcgaatgg gaaaagaacg gtaccccagg
      541 cgagcaacgc aatatcgcct tcaataggct gaaaatatgt ttacaaaatc aagaggcaga
      601 attaaattta tctgagttag atttaaaaac attaccagat ttaccgcctc agataacaac
      661 actggaaata agaaaaaacc tattaacaca tctccctgat ttaccaccaa tgcttaaggt
      721 aatacatgct caatttaatc aactggaaag cttacctgcc ttacccgaga cgttagaaga
      781 gcttaatgcg ggtgataaca agataaaaga attaccattt cttcctgaaa atctaactca
      841 tttacgggtt cataataacc gattgcatat tctgccacta ttgccaccgg aactaaaatt
      901 actggtagtt tctggaaaca gattagacag cat
//
LOCUS       DAJTMX010000334          870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTMX010000334 DAJTMX010000000
VERSION     DAJTMX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.339"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>870
                     /locus_tag="OO145_004387"
     CDS             <1..>870
                     /locus_tag="OO145_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5469234.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAJTMX010000335          868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTMX010000335 DAJTMX010000000
VERSION     DAJTMX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.340"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            56..655
                     /locus_tag="OO145_004388"
     CDS             56..655
                     /locus_tag="OO145_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5469235.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          229 a          215 c          208 g          216 t
ORIGIN      
        1 tcttcaaaaa ttacggcaac gcttcttcct gtgttacaga acacgaggaa gaaacatgca
       61 aacttataac accaatcccg cttacgaaat ggctccccag ctcctggagc actttaacca
      121 gcatctggat tcattattcg gggtctattc gaaactgtta ccgttcagaa tggattttgc
      181 ttaccgaaaa aacaccctca gctacagatg tgcctgcagg tacgccatgt gcgcggagat
      241 gctgcaactg attaacgagg tgggagagaa actggtcggg tatgcctggg tgatggaata
      301 tacagaacgt aaagggcttc acattcattt tgtgggctat ctgaacggcc agagccacag
      361 gagttcgtat ctggtatcca gattgatggg agatatctgg aggcgggtta ccgagggtaa
      421 tggttactac cactggtgcc ggtttaataa aaattatccg gtaaacatta accacgtcat
      481 tcactattca gatcacaaag cggtgaatga tttacgctat gccatcagct acctggccaa
      541 acgggaacag aaagaatgtg gcatcatcct gaaatgcagc gggcttcctg aaaaaagtaa
      601 ccggggtcga ccacgactgg acagccccct gccggggata tgtgctctgg tgtaaacccc
      661 tgaacgacaa gtacgggctg gcgctaatct ttgctgtcgg ttccggcggc cacttcagcg
      721 gcactgcgca ttttttacgg cgtggtcatt ccgtatgaac catacggaga tttaaccatg
      781 acacattctt taccattcct tccggcaacc gatacagcac atcccccaat ctccatctct
      841 gcttactgac tgttttttac gtcagtgg
//
LOCUS       DAJTMX010000336          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTMX010000336 DAJTMX010000000
VERSION     DAJTMX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.343"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..257)
                     /locus_tag="OO145_004389"
     CDS             complement(<1..257)
                     /locus_tag="OO145_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469236.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="OO145_004390"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="OO145_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5469237.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="OO145_004391"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="OO145_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5469238.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          240 a          175 c          220 g          229 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttga
//
LOCUS       DAJTMX010000337          831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTMX010000337 DAJTMX010000000
VERSION     DAJTMX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.344"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..326
                     /locus_tag="OO145_004392"
     CDS             <1..326
                     /locus_tag="OO145_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5469239.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO145_004393"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO145_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5469240.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAJTMX010000338          813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTMX010000338 DAJTMX010000000
VERSION     DAJTMX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.345"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>813
                     /locus_tag="OO145_004394"
     CDS             <1..>813
                     /locus_tag="OO145_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5469241.1"
                     /translation="VIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHI
                     DVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGR
                     STAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKT
                     KITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVD
                     MAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLM"
BASE COUNT          212 a          195 c          227 g          179 t
ORIGIN      
        1 gtgattgaag gcgacctgtc cagttacttc gacaccgtac atcatcgact gctgatgaag
       61 gctgtacgcc gcaggatcag tgacgcacgt ttcatgactc tgctgtggaa aaccatcaag
      121 gcgggacata tcgatgtcgg tctctttcgg gcggccagtg aaggtgtgcc acagggcggt
      181 gttatatcgc cgctattatc gaacatcatg ctgaatgagt tcgatcaata cctgcatgag
      241 cgctacctga gcgggaaagc cagaaaagat cggtggtact ggaataacag tatccaacgg
      301 ggccgaagta cggcggtcag agaaaactgg cagtggaaac ccgcggtggc gtactgccgc
      361 tatgccgatg attttgtcct catcgtcaaa ggcaccaaag cacaggcgga agccatcagg
      421 gaggagtgtc ggggtgtgct cgaaggcagt ctgaaactca ggctgaacat ggataagact
      481 aaaatcaccc atgttaatga cggctttatc tttctggggc acaggatcat tcgcaaacgc
      541 agtcgttatg gcgagatgcg agtggtctca acgatcccgc aggagaaagc cagaaacttc
      601 gccgcatcgc tgacagcact gttatcaggc aactacagtg aaagcaaagt cgatatggct
      661 gaacaactca accgaaaact gaaaggctgg gccatgttct atcagttcgt tgattttaag
      721 gccaaagtct tcagttatat cgaccgtgtc gtgttctgga agctggctca ctggctggcc
      781 cgcaaatacc gtacaggtat cgcttccctg atg
//
LOCUS       DAJTMX010000339          807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTMX010000339 DAJTMX010000000
VERSION     DAJTMX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.346"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            211..462
                     /locus_tag="OO145_004395"
     CDS             211..462
                     /locus_tag="OO145_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469242.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            529..807
                     /locus_tag="OO145_004396"
                     /pseudo
     CDS             529..807
                     /locus_tag="OO145_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          216 a          191 c          197 g          203 t
ORIGIN      
        1 ggcgagtgag aaatccctcg ccacctctga tgtggcaggc atcctcaacg cacccgcact
       61 taacccgctt cggcgggttt ttgtttttat tttcaacgca tttgaagttc tggatggtgc
      121 cggaatagaa tcaaaaatac ttaagtagcg cgcagggata aaagggatgg accccgaaca
      181 ggggagtgct acttatctgg aaggattctg atgatgaaca tcgaagaact gcgtaaaatt
      241 ttttgtgaag atggcctcta tgctgtgtgc gttgaaaatg gaaatattgt tagtcattac
      301 cgcattgtgt gtttgcaaaa aaatggggct gcgttaatta attttgtgga tgcccgagtg
      361 acggacggat ttatcttgcg cgacggtgag tttgtcactt cattacaggc attgaaagag
      421 atcggaataa aagctggctt ttctgctttt tcagaagaat aaactcatct acaatcttgc
      481 gcggggctga actcccgctg agtaacaccg tgccaccgga gaaaaccgat ggcacgcaac
      541 gcaaaatatt acaatcatga taattcgacc gttcttgccc acacgcacga gcggtattct
      601 cacgcattta agtcagactg gtaccagcat ccctcatgca ctgaagaaca ggccgaatgg
      661 cttattcagt gttaccgcag gcgcggatgc gaggttaaaa aagcccttag cctcgactac
      721 cgtcactaga taatctccgt caggctccct tactccgaac agccaccgcg tccgtcccgc
      781 acattccagc aacggatctg gaggtaa
//
LOCUS       DAJTMX010000340          773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTMX010000340 DAJTMX010000000
VERSION     DAJTMX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.347"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            85..>773
                     /locus_tag="OO145_004397"
     CDS             85..>773
                     /locus_tag="OO145_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5469243.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          188 a          187 c          241 g          157 t
ORIGIN      
        1 cggcctgaga cgttgctccg acaggttaac accgtctcgt tgccggacag tgcgatcccg
       61 ccaggcttca gggggtaaca gaccatgaaa aatattgcga caggaggcgt tctggagcgt
      121 atccgcagac tgaccccacc acatgtaacc gccccattca gaacggttgc ggagtggcgc
      181 gagtggcaac ttgctgaagg ccagaaacgt tgcgaggaga tcaaccgcct gaatcgtcag
      241 ttgcgggtgg aaaaaatcct gaaccgctcc ggcatccagc cgttgcaccg gaagtgttcg
      301 tttgcgaatt accgggcgca gaacgacggt cagcgacatg cactgagtca ggcgaaatcc
      361 atagctgacg aactgatgac aggctgtacg aattttgtgt tcagcggtaa gcccggcacc
      421 ggaaaaaatc atcttgcagc cgcgattggc aaccggctga tggcgaaggg gcgtagcgtg
      481 attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg agagctacga caacggcaaa
      541 tccggtgaaa aatttttaca ggagctttgt ggtgttgacc tgctggtcct ggatgaaatt
      601 ggcatgcagc gggatacgaa aaacgagcag gtggtactga accagattgt tgatcgccgg
      661 acggcatcgt tacgcggtgt ggggatgctg acaaatatta accatgcagc gatgaataca
      721 cttctcggcg agcgggtgat ggatcgcatg gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTMX010000341          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTMX010000341 DAJTMX010000000
VERSION     DAJTMX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.348"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            459..>763
                     /locus_tag="OO145_004398"
     CDS             459..>763
                     /locus_tag="OO145_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5469244.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          259 a          152 c          127 g          225 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaa
//
LOCUS       DAJTMX010000342          759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTMX010000342 DAJTMX010000000
VERSION     DAJTMX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.349"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>759
                     /locus_tag="OO145_004399"
     CDS             <1..>759
                     /locus_tag="OO145_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5469245.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAJTMX010000343          729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTMX010000343 DAJTMX010000000
VERSION     DAJTMX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.350"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>729
                     /locus_tag="OO145_004400"
     CDS             <1..>729
                     /locus_tag="OO145_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469246.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASR"
BASE COUNT          165 a          189 c          233 g          142 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggc
//
LOCUS       DAJTMX010000344          715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTMX010000344 DAJTMX010000000
VERSION     DAJTMX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.351"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(54..715)
                     /locus_tag="OO145_004401"
                     /pseudo
     CDS             complement(54..715)
                     /locus_tag="OO145_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          206 a          146 c          138 g          225 t
ORIGIN      
        1 cagtgggtat atctgttcag aatatttcac gtagcacacg gatatataag aaatcattct
       61 tcctctggat agcttgaact taagagcata tcctgcgtga attggtcatt agctgattga
      121 ttgaagctaa agaaagatgg tgcatccggt ctaacagttg cttcaccagt gatattaact
      181 gataaaggct tcttagtttc tgctgttaaa gcgtctacct ttcttcctaa ctctgataca
      241 gccatagcta attgagcatt gctattaacc atgtcacctg tgatagctga tggagttgat
      301 tgtgtgttat ttacgaagtt attcatcggt tcttggaagt tagcaatttt attagttgca
      361 ccattaaacc agtcagcgat agaactattg gcttagatga atcacctttg tattcagggt
      421 tgaagtttgt ttctgtgcca atacctgatt gatgtaagtt cggattctta gctgcttcag
      481 ggtttaaatt atcaagagca aaatttacca actcagcata aaggacaact ctaccgactt
      541 tatttagcgc accacctgaa gaaccagcct tgccagcttt agctttatta acagcgtcac
      601 cgaatgaagt aacagcgttt gcaaggttta acaagcctcc tgctacattt gcagcctttt
      661 gagcagtaac agccacagca agcatcttca gaccatcaat taacccatca gataa
//
LOCUS       DAJTMX010000345          701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTMX010000345 DAJTMX010000000
VERSION     DAJTMX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.352"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..440)
                     /locus_tag="OO145_004402"
     CDS             complement(<1..440)
                     /locus_tag="OO145_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5469247.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYP"
BASE COUNT          213 a          171 c          131 g          186 t
ORIGIN      
        1 tctggataat cggcatgagc aataacatgc ttgccggtgc gactgtgtac ttcaagaata
       61 aagggcaaat gctgacattt ttccagcgcc gttttcgctt gtttctgttg attatctgcc
      121 agcgcaataa accagtcgcc gccattcatc aaccacaaag acatctgctg gaatgccagc
      181 gcatccatcg ccatctgttc atgattgcct cttaccgcac gaacccaatg ttgttccagt
      241 aactgcagac aacgtaaact ttgcggccca cgatcgataa cgtctcccac tgagataagt
      301 aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg ctcaagacaa
      361 ccgtgtatat cgccagaaag ccagatatgt cgccattgat gacccgcaat tctctgataa
      421 acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca taatcttaac
      481 aagaatgtta aaaaacgctg gactcagaca gtagagtgtg tgttatggtt gactataaag
      541 tcagcgaagg aaatgcttct ggcttttaac agataaaaag agaccgaaca tgattcctgt
      601 tttcgtcaac aaacaataaa accctttaaa attaatgcgt tgaataaatt tacgttcatc
      661 ttttaatccc tgacacgtac cattgcatat taatacattc a
//
LOCUS       DAJTMX010000346          694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTMX010000346 DAJTMX010000000
VERSION     DAJTMX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.353"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            32..493
                     /locus_tag="OO145_004403"
     CDS             32..493
                     /locus_tag="OO145_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5469248.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>694)
                     /locus_tag="OO145_004404"
     CDS             complement(534..>694)
                     /locus_tag="OO145_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5469249.1"
                     /translation="LAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLA
                     ARQHVKKS"
BASE COUNT          204 a          152 c          159 g          179 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaa
//
LOCUS       DAJTMX010000347          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTMX010000347 DAJTMX010000000
VERSION     DAJTMX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.354"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(135..265)
                     /locus_tag="OO145_004405"
                     /pseudo
     CDS             complement(135..265)
                     /locus_tag="OO145_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(263..358)
                     /locus_tag="OO145_004406"
                     /pseudo
     CDS             complement(263..358)
                     /locus_tag="OO145_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          125 c          166 g          200 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt
       61 tattttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg
      121 ggtggcgctt ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt
      181 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
      241 ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg
      301 tcacctgttt atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc
      361 cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
      421 ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg
      481 agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga
      541 ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg
      601 acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct
      661 attgagcttt atttcaaata tggtaagaag c
//
LOCUS       DAJTMX010000348          684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTMX010000348 DAJTMX010000000
VERSION     DAJTMX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.355"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(6..>684)
                     /locus_tag="OO145_004407"
     CDS             complement(6..>684)
                     /locus_tag="OO145_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5469250.1"
                     /translation="GYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRG
                     EVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
                     PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCL
                     DNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRN
                     QTYMPRV"
BASE COUNT          180 a          157 c          133 g          214 t
ORIGIN      
        1 gacagttata cacgaggcat ataggtctga ttccgatatt caattggagt cagacctttt
       61 aatttcaggc taattcttct gctgttatag tattcaatat attccgtaac agcatccttc
      121 agttcgctta tattactgaa ctcatcaaga taaaaacact ccgactttaa ggttccaaag
      181 aaacactcca ccacagcatt atccagacaa ttgccttttc tggacatgct ttgtttaata
      241 ccatgttctt taaggatatt ttgatatctt ctcatacgat actgccatcc ctggtcagag
      301 tgcagaacag gatgctcgtg aggattaagc tttttgaatg cctgatcgag catattctca
      361 accatgttca tcactggtct ttccgaaagg ctgtaagaaa taacttcgtt gttgaagaga
      421 tctattactg gagacaaata cagcttgcgc ccattgactg caaattcagt aacatcggta
      481 acccacttct cgtttggccg cgtagccttg aaatctcttt ggagaacatt aggggcggtt
      541 tgccctacct ctcctctgta agagcggtat cgcttgacct taatcgctgc tttaagtgag
      601 agggttccca tcaggcgctg aacagcttta tggttaatct gtttcccttc tcgatgaaga
      661 gacagcgtta ccctacggta tccg
//
LOCUS       DAJTMX010000349          679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTMX010000349 DAJTMX010000000
VERSION     DAJTMX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.356"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            46..679
                     /locus_tag="OO145_004408"
                     /pseudo
     CDS             46..679
                     /locus_tag="OO145_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          204 a          168 c          172 g          135 t
ORIGIN      
        1 tgaacagagg tgatatgctc acctcagaac aacacaggtg ctccaatgaa aaaaagaaat
       61 tttagcgcag agtttaaacg cgaatccgct caactggttg ttgaccagaa atacacggtg
      121 gcagatgccg ccaaagctat ggatgttggc ctttccacaa tgacaagatg ggtcaaacaa
      181 ctgcgtgatg agcgtcaggg caaaacacca aaagcctccc ccattacccc ggaacaaatt
      241 gaaatccgtg agctcaggaa aaagctacaa cgcattgaaa tggagaatga aatattaaaa
      301 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ctcgataatc gggaaactca
      361 gagcgcatta tcctgtggtc acactctgcc atgtgttcgg ggttcatcgc agcagctaca
      421 gatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagtcagg
      481 tacttgagct acatggcatc agccacggtt cggccggagc aagaagcatc gccacaatgg
      541 caacccggag aggctaccag atgggacgct ggcttgctgg caggctcatg aaagagctgg
      601 ggctggtcag ctgtcagcag ccgactcacc ggtataaacg tggtggtcat gaacatgttg
      661 ctatccctaa ctaccttga
//
LOCUS       DAJTMX010000350          678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTMX010000350 DAJTMX010000000
VERSION     DAJTMX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.357"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..678
                     /locus_tag="OO145_004409"
                     /pseudo
     CDS             1..678
                     /locus_tag="OO145_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          146 a          168 c          215 g          149 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca gtgaaacagt
      541 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc
      601 agtggagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac gccagttcgc
      661 ccggatgttg ggacttga
//
LOCUS       DAJTMX010000351          678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTMX010000351 DAJTMX010000000
VERSION     DAJTMX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.358"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..432
                     /locus_tag="OO145_004410"
     CDS             <1..432
                     /locus_tag="OO145_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5469251.1"
                     /translation="LNDIVSSPSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYL
                     TAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKY
                     VQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            433..>678
                     /locus_tag="OO145_004411"
     CDS             433..>678
                     /locus_tag="OO145_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCR5469252.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQ"
BASE COUNT          168 a          173 c          185 g          152 t
ORIGIN      
        1 ctgaacgata tcgttagttc tccttcactt aagagcatta caggcttacg tggagtaatc
       61 cccaacgttc caggctcaca ggctgcagac actcaggcac gacttgatac ctttaaatca
      121 caagcatacc tgacagcggt tcaggccatg cgaggcatgg gcgcactttc tgatgccgag
      181 ggcaaaaagc tcgaccaggc tgttggttcg ctgcagaact cgcagagcga ggagtccttt
      241 cgtcgcaacg ctggcgtcat cctgaacacg ctcaaccaga agcgtaatga ggcggttggt
      301 aagtacgttc agcaaaacgg tatcaagcga gtggaagcgc ctcaggcttc tatagattac
      361 ctgaagcagc accccgagct gtcaatcgac ttcattaatc gctacggata tcttccatct
      421 ttggggcagt aaatggctaa ttaccgtgat ttgttagagc aggctggcgc acgttacggt
      481 gtgccagaag ggttgatgac tgcactgggt gccaaggagt cttcttacaa ccctgccgca
      541 gtaagctccg ccggggctgt aggattgact caggtcatgc ctgggacatg gcgtgatatg
      601 ggttataccg atgagcaaat gcaaaacccc gaatatcagg ctgacgctgg cgcgcgctat
      661 ctggcaaaga tgtaccag
//
LOCUS       DAJTMX010000352          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTMX010000352 DAJTMX010000000
VERSION     DAJTMX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.359"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>668
                     /locus_tag="OO145_004412"
     CDS             <1..>668
                     /locus_tag="OO145_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5469253.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTMX010000353          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTMX010000353 DAJTMX010000000
VERSION     DAJTMX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.361"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            103..>659
                     /locus_tag="OO145_004413"
     CDS             103..>659
                     /locus_tag="OO145_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5469254.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTMX010000354          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTMX010000354 DAJTMX010000000
VERSION     DAJTMX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.362"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            52..>656
                     /locus_tag="OO145_004414"
     CDS             52..>656
                     /locus_tag="OO145_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5469255.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLP"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAJTMX010000355          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTMX010000355 DAJTMX010000000
VERSION     DAJTMX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.363"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(151..648)
                     /locus_tag="OO145_004415"
     CDS             complement(151..648)
                     /locus_tag="OO145_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5469256.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAJTMX010000356          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTMX010000356 DAJTMX010000000
VERSION     DAJTMX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.364"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(24..>649)
                     /locus_tag="OO145_004416"
     rRNA            complement(24..>649)
                     /locus_tag="OO145_004416"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJTMX010000357          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTMX010000357 DAJTMX010000000
VERSION     DAJTMX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.365"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            18..491
                     /locus_tag="OO145_004417"
     CDS             18..491
                     /locus_tag="OO145_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5469257.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTMX010000358          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTMX010000358 DAJTMX010000000
VERSION     DAJTMX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.366"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..249)
                     /locus_tag="OO145_004418"
     CDS             complement(<1..249)
                     /locus_tag="OO145_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR5469258.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(270..>623)
                     /locus_tag="OO145_004419"
     CDS             complement(270..>623)
                     /locus_tag="OO145_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5469259.1"
                     /translation="STVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLR
                     HRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNR
                     RLSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ttgcaggtaa gtcagtaatg tttcttgctg atcgcgccag acgttctgca catcaacatt
       61 ttcattactg agcagtattt taactttgct gacctgcacg ccgttgtcga tccagcgttt
      121 gatctcgcgg atccggtcaa tatcggcatc gttgaacagc cgatgaccgc cgtctgtccg
      181 ttgcggtttc agcaatccgt aacgcctctg ccacgcgcgt aacgtgacag ggattaaagg
      241 taaaccaatt gattttgcaa gtttttattt taccccgctt tcttatgggg catgcatggg
      301 acactttcag atagtcttct gttaagaagt tctatctgtt cgtgattgtt gtctttcatc
      361 catgctccgt aaacattgaa taccatttgt gcgtttgtgt ggcccatctg gcttgcgata
      421 aaactaggat tagctcctgc ggcaagtgac cagcatgcat aggtatgcct ggattgatac
      481 gattttctgt gtctcagacc tgcgcgtttt aagatacttg tccatgactc cctgatggag
      541 tcaaccttat agtgcggtcc ggaaaactgc cgctgtttta ttacctgagg actaaaaaca
      601 aaagtgcatt tatgcacagt act
//
LOCUS       DAJTMX010000359          608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTMX010000359 DAJTMX010000000
VERSION     DAJTMX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.367"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>608)
                     /gene="ospC4"
                     /locus_tag="OO145_004420"
     CDS             complement(<1..>608)
                     /gene="ospC4"
                     /locus_tag="OO145_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5469260.1"
                     /translation="DKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLN
                     KTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVS
                     RYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRII
                     NFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEIN"
BASE COUNT          193 a          104 c           90 g          221 t
ORIGIN      
        1 tgttaatctc ttcataatta gatgctttaa tcgcatcctc caaagatatg tctctgagtt
       61 ttattttttt gaagttatct gtacttagca ttcttggtat atgatactca agctgaaaca
      121 caaagtttat aattctatcc agactaacag gatcaatatt tttgttataa caaaactctc
      181 tgaatccttg atctttactc tttcttataa aatcaataag atgcaaccca attccaagcc
      241 tcatatcttt agtgttaaat attggaacat cagttttccc ctgattacca tgcacatacc
      301 tgctaacttc atccacaacc tctttaaagt tatctaagaa aaatgattgg tgctcatggt
      361 aaaaaacagg tggaatagca ttatcaaagt gatcggttaa tgtcatatat ccatatggag
      421 agtcatgatc aatgatatac gcattggcgc caaaatctac agtatgatgt gttttattta
      481 gtgggagttt ttcttgatga ttggaaattt cgaccccaaa aaaaacaaaa tcgtggttag
      541 agatttcttt aatatcctca gagtatgtat tcttatgtgt ttgtatattt tcagactgaa
      601 ttttatca
//
LOCUS       DAJTMX010000360          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTMX010000360 DAJTMX010000000
VERSION     DAJTMX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.368"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT          217 a          105 c          120 g          148 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 gggggatgaa aatacctgaa gcagtaaatc atattaatgt tcagaataat attgatcttg
      541 ttgatggaaa aacgaatccg aataaagcaa caaaagcatt acaaaaaaat
//
LOCUS       DAJTMX010000361          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTMX010000361 DAJTMX010000000
VERSION     DAJTMX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.369"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(141..>581)
                     /locus_tag="OO145_004421"
     CDS             complement(141..>581)
                     /locus_tag="OO145_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5469261.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAJTMX010000362          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTMX010000362 DAJTMX010000000
VERSION     DAJTMX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.370"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..98
                     /locus_tag="OO145_004422"
     CDS             <1..98
                     /locus_tag="OO145_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5469262.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO145_004423"
     CDS             156..512
                     /locus_tag="OO145_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469263.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTMX010000363          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTMX010000363 DAJTMX010000000
VERSION     DAJTMX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.371"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            28..>575
                     /locus_tag="OO145_004424"
     CDS             28..>575
                     /locus_tag="OO145_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469264.1"
                     /translation="MTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPA
                     SSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGS
                     RAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLL
                     DNVWTVPKEHSKMGNVIRRPIF"
BASE COUNT          156 a          134 c          151 g          134 t
ORIGIN      
        1 tattttgaag agaaggtgga gaactccatg acggtcgccc agtgtctcga ctactggttc
       61 gacaactacg tctctacaac tctcagagaa aagacccagg cactttatcg atcaacggtt
      121 atgaagcgca tgcatgacgc cttccctaat cgtccggcat cttctatcac ggttaagcaa
      181 tgggttgacc tgcttaccga agaagaaaaa gataatccac gccgagcaag gcaggtgcta
      241 agtcaactaa gatcagcaat tagttggtgc atgcggcgtc agttgataga tagttgcgca
      301 attatgagca tccagccaag ggacttcggc tcccgcgctg aggtagggga tcgggtactg
      361 tcgtatcacg aactggctaa gatttggctt gctattgaaa gaagccgtgc gtctacgtca
      421 aataagctcc ttcatcagat gcttatgctg tggggggcga ggctctcaga gcttaggctg
      481 gcaacaaaga cagaatttga cctgctggac aacgtatgga ccgtaccgaa agagcatagc
      541 aagatgggta atgttatccg ccgtccaatc ttcga
//
LOCUS       DAJTMX010000364          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTMX010000364 DAJTMX010000000
VERSION     DAJTMX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.372"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..297
                     /locus_tag="OO145_004425"
     CDS             <1..297
                     /locus_tag="OO145_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5469265.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="OO145_004426"
     CDS             complement(405..>566)
                     /locus_tag="OO145_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469266.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAJTMX010000365          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTMX010000365 DAJTMX010000000
VERSION     DAJTMX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.373"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT          146 a          113 c          120 g          180 t
ORIGIN      
        1 atggcttcac ccgtgttaat atcctggata actgtgtttg aaggatttct gttaataatc
       61 tgctcctgca actcacctga tgagtattag tggtaataca taatcttact cgtaaaagag
      121 tataccttat gtcattaaac agtcttccgg cgtgttgttc aatcctgtac ctctgttact
      181 tatctggtac gttgatatgt gtaatgctac gttaattgca atatctggtt atggctttat
      241 accttcagca tttaaaatat tttattaccg gagaaacagg gaagtggaaa gactttcggg
      301 ataatgtcag gccattctga cgttaatcac agtaccaaat aacgagaggg taatggaagt
      361 taaaacctgc gtaagttcct ctgttgttca ctggaaaaag tgattgccca cacgcgttat
      421 aaaggccttt ccgctgcagc ctggttaaca gagcttcagg cgttatatgc gctgtatggc
      481 tgttacgtat gctgttcttt gcctggttct gatacccgtg gaagtcgtta ccggaggaaa
      541 cacgttcacc ctactacct
//
LOCUS       DAJTMX010000366          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTMX010000366 DAJTMX010000000
VERSION     DAJTMX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.374"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>559
                     /locus_tag="OO145_004427"
     CDS             <1..>559
                     /locus_tag="OO145_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5469267.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          165 a          138 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagccca
//
LOCUS       DAJTMX010000367          553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTMX010000367 DAJTMX010000000
VERSION     DAJTMX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.375"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>553)
                     /locus_tag="OO145_004428"
     CDS             complement(<1..>553)
                     /locus_tag="OO145_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR5469268.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRHLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          133 a          119 c          135 g          166 t
ORIGIN      
        1 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagactgc
       61 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
      121 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
      181 tctgactgat cgcttgggcg acggtgtttt gccattctaa agaacaggtg ctgaagtcaa
      241 atgtggctgt tttattatcc cgttgcgaca cgctacaggc taagtgacgt aattgggtca
      301 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
      361 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccaaggtaaa acagcatctg
      421 tatttttgtc agccgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      481 ataagtccgc taccttgaga gagtccgtta ccgtttggga aggttgcaaa gaattatctt
      541 tcgcttgctc cac
//
LOCUS       DAJTMX010000368          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTMX010000368 DAJTMX010000000
VERSION     DAJTMX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.376"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            101..>550
                     /locus_tag="OO145_004429"
     CDS             101..>550
                     /locus_tag="OO145_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469269.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLPDWLSETLWHEWVQFRQALRKPIRTELGAN"
BASE COUNT          130 a          119 c          170 g          131 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac
//
LOCUS       DAJTMX010000369          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTMX010000369 DAJTMX010000000
VERSION     DAJTMX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.377"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT          173 a           78 c          107 g          175 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DAJTMX010000370          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTMX010000370 DAJTMX010000000
VERSION     DAJTMX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.378"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>523)
                     /locus_tag="OO145_004430"
     CDS             complement(<1..>523)
                     /locus_tag="OO145_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5469270.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNVDNAMQ"
BASE COUNT          149 a          124 c          104 g          146 t
ORIGIN      
        1 tctgcatagc attatcaaca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc aca
//
LOCUS       DAJTMX010000371          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTMX010000371 DAJTMX010000000
VERSION     DAJTMX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.379"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            368..>519
                     /locus_tag="OO145_004431"
     CDS             368..>519
                     /locus_tag="OO145_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5469271.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          137 c          113 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctatag gtctctctag ggtctcctct
       61 aggcattacc cccagaagct aactctatgg ccctctatgg tcctctatgg ccctgtacgt
      121 cattctttag gatgccctgt agtttcgtat agcctgatgc ctagagagac cctagagaga
      181 ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca tcgtcatcat
      241 catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc
      301 gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa
      361 tcaagcgatg tctcaacaac cacaacaacc acaacaagaa atcagcatca ttaacctcga
      421 ccagcttgtg tccatgacct cagttgaaat tgctgagctg acaggcaagg agcaccgaaa
      481 tgtccttcgt gatattcgta agatgctcga agaacttaa
//
LOCUS       DAJTMX010000372          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTMX010000372 DAJTMX010000000
VERSION     DAJTMX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.380"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..511
                     /locus_tag="OO145_004432"
                     /pseudo
     CDS             1..511
                     /locus_tag="OO145_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJTMX010000373          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTMX010000373 DAJTMX010000000
VERSION     DAJTMX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.381"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..163
                     /locus_tag="OO145_004433"
     CDS             <1..163
                     /locus_tag="OO145_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5469272.1"
                     /translation="PLLPVHLKAGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWP
                     SRYMEKTSC"
     gene            343..505
                     /locus_tag="OO145_004434"
                     /pseudo
     CDS             343..505
                     /locus_tag="OO145_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068751.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          145 a          117 c          121 g          122 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaaggcagg cttaaagcct gacactatgc gtaacgtatt
       61 tcgctgccat ataccacgat acgaagcaat cattgctaga tatttggata tggaaccagc
      121 ccagatttgg ccttctcggt atatggagaa aacatcatgt taacccattg ggtaacagcg
      181 caagaatgcg taggaatgtc atgctttcct aaacatgagg tgagtattcg ccgcaatctt
      241 gaaaagcttg cagccaatca tccagaacta cgtcgcaagc gtacgggttc aaaggctttg
      301 gaatttcata tatcagttct accagcggca gcacgtgctg agttgttatt aaaatctgag
      361 caaatagaaa caagctaggg gtattttgaa atcgcccgcc ccacgctgga agcgcacgat
      421 tacgatcgtg aggcactgtg gagcaaatgg gataaagcca gcgattccca gcgcagactt
      481 gctgaaaaat ggttgcctgc ggttc
//
LOCUS       DAJTMX010000374          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTMX010000374 DAJTMX010000000
VERSION     DAJTMX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.382"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            88..153
                     /locus_tag="OO145_004435"
                     /pseudo
     CDS             88..153
                     /locus_tag="OO145_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..>504
                     /locus_tag="OO145_004436"
     CDS             381..>504
                     /locus_tag="OO145_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5469273.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVI"
BASE COUNT          161 a          101 c          118 g          124 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcg
//
LOCUS       DAJTMX010000375          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTMX010000375 DAJTMX010000000
VERSION     DAJTMX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.383"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>484
                     /locus_tag="OO145_004437"
     CDS             <1..>484
                     /locus_tag="OO145_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5469274.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DAJTMX010000376          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTMX010000376 DAJTMX010000000
VERSION     DAJTMX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.384"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT          135 a           98 c           89 g          158 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tctgataaat atttatttca cgccgtatat
      301 ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata
      361 tatggttatt tttcaccctg cgtattcagc acgcaacaaa aaacccgccg aagcgggttt
      421 agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg cgagggattt ctcccccgcc
//
LOCUS       DAJTMX010000377          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTMX010000377 DAJTMX010000000
VERSION     DAJTMX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.385"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>470
                     /locus_tag="OO145_004438"
     CDS             <1..>470
                     /locus_tag="OO145_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469275.1"
                     /translation="ESGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDS
                     IITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNT
                     GNTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          104 g          122 t
ORIGIN      
        1 tgaatccggt agtaccctta tctcacgaga taataagctg gtggagaact tcatcgacac
       61 tcttgaaaac tggcgtgacc agaagcagat tatttcagtt atctgtaaag atggtattaa
      121 gattgacgac tccattatta caagctttaa agcctctaaa gatgttggaa tttcaaacgg
      181 actgagaatc cagctaactt ttcaggaaat taacttcaaa gctattgtag ggcaaactga
      241 tgtttcagca gccactggac ggacttctac cacgaatgat ggcggtgcta ccagtaaaaa
      301 gaacactggc aacactacga caagtcttgg aagccctatg ctgacctgta aagagctttt
      361 tagctattcg gcaagtgagt tatcagatga agcgttaaaa gcccgtgtaa cgtgctctaa
      421 gagcgttagc gttaaaaatg gtgagagcac cttcacagcc tcagctaatg
//
LOCUS       DAJTMX010000378          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTMX010000378 DAJTMX010000000
VERSION     DAJTMX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.386"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..465
                     /locus_tag="OO145_004439"
                     /pseudo
     CDS             1..465
                     /locus_tag="OO145_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          111 a          133 c          136 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaa
//
LOCUS       DAJTMX010000379          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTMX010000379 DAJTMX010000000
VERSION     DAJTMX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.387"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>463
                     /locus_tag="OO145_004440"
     CDS             <1..>463
                     /locus_tag="OO145_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5469276.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTMX010000380          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTMX010000380 DAJTMX010000000
VERSION     DAJTMX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.388"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>461)
                     /locus_tag="OO145_004441"
     CDS             complement(<1..>461)
                     /locus_tag="OO145_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCR5469277.1"
                     /translation="ILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          107 a          117 c           90 g          147 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaat a
//
LOCUS       DAJTMX010000381          455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTMX010000381 DAJTMX010000000
VERSION     DAJTMX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.389"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>455)
                     /locus_tag="OO145_004442"
     CDS             complement(<1..>455)
                     /locus_tag="OO145_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5469278.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          110 a          127 c          124 g           94 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccg gtacagatga actcatacgg ccccagtttg
      301 gcgatctcat ccgccagata ggcagcgacc tggtaggaag cattctgtac tttggtatag
      361 ccttcacgac caaggcgcag gaattcatag tactgtgcaa tcacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatctga ccacc
//
LOCUS       DAJTMX010000382          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTMX010000382 DAJTMX010000000
VERSION     DAJTMX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.390"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            101..>450
                     /locus_tag="OO145_004443"
     CDS             101..>450
                     /locus_tag="OO145_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5469279.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          111 a          125 c          116 g           98 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat
//
LOCUS       DAJTMX010000383          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTMX010000383 DAJTMX010000000
VERSION     DAJTMX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.391"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(15..230)
                     /locus_tag="OO145_004444"
     CDS             complement(15..230)
                     /locus_tag="OO145_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5469280.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          146 a          107 c           94 g          101 t
ORIGIN      
        1 taatgatggc gacattattc acctctcgca gccttacgct tgtcttctct gattttgaag
       61 tacagatttg tcagataagt caggaagccc agaaccagac tccccagtac accaatcgca
      121 gcccactgtg acggactgac ctgatcaagc cactgtaaaa accagtagcc agcactgcct
      181 gcggaggtgc cgtaggcaat gcccgttgaa attttgtcca tggatttcat agcctcacct
      241 ccgcaaataa cggatggcgt agttttacac tgagaaatga aagggatttg aaaagaaaaa
      301 accgcaaaag cgggcgaaac gatatataca ataaggaaag cactctatcc aacaaaccac
      361 tcacagttaa tcggaataaa agcagagtgc ttatgaatga tcgcctgccc gaaggttagt
      421 atttctgcac agcaattttg caaaaaaa
//
LOCUS       DAJTMX010000384          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTMX010000384 DAJTMX010000000
VERSION     DAJTMX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.392"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..373)
                     /locus_tag="OO145_004445"
     CDS             complement(<1..373)
                     /locus_tag="OO145_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469281.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           96 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcgg
//
LOCUS       DAJTMX010000385          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTMX010000385 DAJTMX010000000
VERSION     DAJTMX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.393"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            213..>443
                     /locus_tag="OO145_004446"
     CDS             213..>443
                     /locus_tag="OO145_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5469282.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAJTMX010000386          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTMX010000386 DAJTMX010000000
VERSION     DAJTMX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.394"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..269
                     /locus_tag="OO145_004447"
     CDS             <1..269
                     /locus_tag="OO145_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5469283.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>439
                     /gene="tnpB"
                     /locus_tag="OO145_004448"
     CDS             266..>439
                     /gene="tnpB"
                     /locus_tag="OO145_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5469284.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          109 a          114 c          125 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtgg
//
LOCUS       DAJTMX010000387          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTMX010000387 DAJTMX010000000
VERSION     DAJTMX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.395"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..279)
                     /locus_tag="OO145_004449"
     CDS             complement(1..279)
                     /locus_tag="OO145_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469285.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           95 a          103 c          134 g          102 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tccc
//
LOCUS       DAJTMX010000388          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTMX010000388 DAJTMX010000000
VERSION     DAJTMX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.396"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>428
                     /gene="istA"
                     /locus_tag="OO145_004450"
     CDS             <1..>428
                     /gene="istA"
                     /locus_tag="OO145_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HCR5469286.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT          100 a          118 c          105 g          105 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcg
//
LOCUS       DAJTMX010000389          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTMX010000389 DAJTMX010000000
VERSION     DAJTMX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.397"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="OO145_004451"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="OO145_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5469287.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAJTMX010000390          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTMX010000390 DAJTMX010000000
VERSION     DAJTMX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.398"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            155..340
                     /locus_tag="OO145_004452"
                     /pseudo
     CDS             155..340
                     /locus_tag="OO145_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAJTMX010000391          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTMX010000391 DAJTMX010000000
VERSION     DAJTMX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.399"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(40..>388)
                     /locus_tag="OO145_004453"
     CDS             complement(40..>388)
                     /locus_tag="OO145_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5469288.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAJTMX010000392          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTMX010000392 DAJTMX010000000
VERSION     DAJTMX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.400"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(143..337)
                     /locus_tag="OO145_004454"
     CDS             complement(143..337)
                     /locus_tag="OO145_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5469289.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT          107 a           96 c           69 g          114 t
ORIGIN      
        1 taaaaatgct caatgcttcg atatcgataa aacattcaag ataggcgcgc cgtgtatttt
       61 aattctttta agactcttca aaaatgtcat cgtagccttt aaaaacgaat tttaaaggca
      121 ccctaaaggc aggtcaatga agttaacccg ccttcgcgtt tacactctct gagagaatac
      181 tagcttcaat ctcttccaaa atctctctga cttgctcgtc gggcatatca acgagcatca
      241 acgctgccag gcgtttacgg ccctctagtt gcagtagctc ttgagtttcc ttatctaaca
      301 agcgtagcac agtctcaaca cctttccttg cgagcatgtt tgtaagggct tctcgttcct
      361 cactggtaag aaacgacaac atttct
//
LOCUS       DAJTMX010000393          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTMX010000393 DAJTMX010000000
VERSION     DAJTMX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.401"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(1..250)
                     /locus_tag="OO145_004455"
                     /pseudo
     CDS             complement(1..250)
                     /locus_tag="OO145_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          122 c           86 g           86 t
ORIGIN      
        1 cactgtctcc gttatggcgt acaccacatc ttccagtaat cgccgcttca ccggactcag
       61 gaccccgtat caccatccgg aaccccttat ccgccacatg ccaggagaac ctttccataa
      121 actcgtcgcc actcatcaca aggttttctt ctcgttttgt ccggtggctg ttgtaacgca
      181 ggccaatttc atcctgaccg gcataatgtt ccaggcggct catctgcacc ggctgcttct
      241 ccagccagtt aagccatcgg tttccctgct gaaaaatatc ggcaaaacgg ggaagcatca
      301 gaaaggcgga ataacgccat ccggccagtg aaccgtaccg cattcagggc aatacatgcc
      361 gtcggcgttg ataccggcag ga
//
LOCUS       DAJTMX010000394          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTMX010000394 DAJTMX010000000
VERSION     DAJTMX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.402"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(273..>381)
                     /locus_tag="OO145_004456"
     CDS             complement(273..>381)
                     /locus_tag="OO145_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5469290.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DAJTMX010000395          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTMX010000395 DAJTMX010000000
VERSION     DAJTMX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.403"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>375
                     /locus_tag="OO145_004457"
     CDS             <1..>375
                     /locus_tag="OO145_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5469291.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAJTMX010000396          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTMX010000396 DAJTMX010000000
VERSION     DAJTMX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.404"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..311)
                     /locus_tag="OO145_004458"
     CDS             complement(<1..311)
                     /locus_tag="OO145_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5469292.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTMX010000397          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTMX010000397 DAJTMX010000000
VERSION     DAJTMX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.405"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(31..>360)
                     /locus_tag="OO145_004459"
     CDS             complement(31..>360)
                     /locus_tag="OO145_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5469293.1"
                     /translation="VNVHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWR
                     YILTNTTVQCVTSFAARKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELI
                     DFLNHGR"
BASE COUNT           87 a           92 c           86 g           95 t
ORIGIN      
        1 ttctgcccag cttaaatgca tataacatgg ctatctcccg tgattcagga agtcgattag
       61 ttcttcgcgt gtggcgctgt aaaaagtcac gtcatccacg cctttgaagt atttcttgat
      121 ggttcctaca cgcttaaggc caatcattgc gcagtacatc tgaccgtgtc ggaatttgcg
      181 tgcagcgaac gatgttacgc actgaacggt ggtgttagtc agaatgtatc gccagaacgc
      241 cagcccaatt tccttgctga agccgcgaat ctctggcagg tacatggcgt ggcaatctaa
      301 tgtcagcggc tgaatctcct gatagtaaac aatgccgcca aactgaccgt gcacgttcac
//
LOCUS       DAJTMX010000398          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTMX010000398 DAJTMX010000000
VERSION     DAJTMX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.406"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..264
                     /locus_tag="OO145_004460"
     CDS             <1..264
                     /locus_tag="OO145_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5469294.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="OO145_004461"
     CDS             288..>353
                     /locus_tag="OO145_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5469295.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAJTMX010000399          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTMX010000399 DAJTMX010000000
VERSION     DAJTMX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.407"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OO145_004462"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OO145_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5469296.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJTMX010000400          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTMX010000400 DAJTMX010000000
VERSION     DAJTMX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.408"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>344)
                     /locus_tag="OO145_004463"
     CDS             complement(<1..>344)
                     /locus_tag="OO145_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5469297.1"
                     /translation="QDEQGRFSICSRQAAVVQRLMGILSLKAAIKVKRYRSYRGEVGQ
                     TAPYVLQRDFKATRPNEKWVTDFTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMN
                     MVENMLDQAFKKL"
BASE COUNT           83 a           88 c           80 g           93 t
ORIGIN      
        1 agctttttga atgcctgatc gagcatattc tcgaccatgt tcatcactgg tctttccgaa
       61 aggctgtaag aaataacttc gttgttgaag agatctatta ctggagacaa atacagcttg
      121 cgcccattga ctgcaaattc agtaaaatcg gtaacccact tctcgtttgg ccgcgtagcc
      181 ttgaaatctc gttggagaac atagggggcg gtttgcccta cctctcctct gtaagagcgg
      241 tatcgcttga ccttaatcgc tgccttaagt gagaggattc ccatcaggcg ctgaaccacg
      301 gcagcctgac ggctgcaaat tgaaaaccgc ccctgttcgt cctg
//
LOCUS       DAJTMX010000401          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTMX010000401 DAJTMX010000000
VERSION     DAJTMX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.409"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            4..171
                     /gene="xisR"
                     /locus_tag="OO145_004464"
     CDS             4..171
                     /gene="xisR"
                     /locus_tag="OO145_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5469298.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            173..>340
                     /locus_tag="OO145_004465"
     CDS             173..>340
                     /locus_tag="OO145_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR5469299.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           82 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc
//
LOCUS       DAJTMX010000402          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTMX010000402 DAJTMX010000000
VERSION     DAJTMX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.410"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>340)
                     /locus_tag="OO145_004466"
     CDS             complement(<1..>340)
                     /locus_tag="OO145_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5469300.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJTMX010000403          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTMX010000403 DAJTMX010000000
VERSION     DAJTMX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.411"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>340)
                     /locus_tag="OO145_004467"
     CDS             complement(<1..>340)
                     /locus_tag="OO145_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5469301.1"
                     /translation="QARYQSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY
                     NNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAI
                     AGAGLASLLGT"
BASE COUNT           69 a           98 c           91 g           82 t
ORIGIN      
        1 gttcccaaaa ggctggcaag acctgcaccg gcaatcgcac cagacgtgcc accgctaatt
       61 gcagtctgaa gacttgatgg tttattggca ttagcagcgg caagagctgc gctttgctgt
      121 gcaatgctgc tcatgttgtt ggcgtacgtc tgcccggcgt ttgcctgacc ttgcagcgca
      181 ccaagcccaa cgtttgccag attgttgtaa ttgctcatct gatttgataa ccaagactga
      241 ccgagtgtcg gcgcgatcgt agccagttga ttgcttgtgg ctgtcgaacc aagtccaccc
      301 gtcgcctccg cagcagcaag actctggtaa cgcgcctgcc
//
LOCUS       DAJTMX010000404          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTMX010000404 DAJTMX010000000
VERSION     DAJTMX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.412"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>335)
                     /locus_tag="OO145_004468"
     CDS             complement(<1..>335)
                     /locus_tag="OO145_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5469302.1"
                     /translation="DIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KQGPYEFICT"
BASE COUNT           84 a           95 c           88 g           68 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatc
//
LOCUS       DAJTMX010000405          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTMX010000405 DAJTMX010000000
VERSION     DAJTMX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.413"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>335
                     /locus_tag="OO145_004469"
     CDS             <1..>335
                     /locus_tag="OO145_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5469303.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAJTMX010000406          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTMX010000406 DAJTMX010000000
VERSION     DAJTMX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.414"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            51..>332
                     /locus_tag="OO145_004470"
     CDS             51..>332
                     /locus_tag="OO145_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5469304.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTMX010000407          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTMX010000407 DAJTMX010000000
VERSION     DAJTMX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.415"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(43..330)
                     /locus_tag="OO145_004471"
     CDS             complement(43..330)
                     /locus_tag="OO145_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469305.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           66 g           89 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat c
//
LOCUS       DAJTMX010000408          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTMX010000408 DAJTMX010000000
VERSION     DAJTMX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.416"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           70 a           92 c           79 g           84 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaa
//
LOCUS       DAJTMX010000409          324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTMX010000409 DAJTMX010000000
VERSION     DAJTMX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.417"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT          110 a           56 c           60 g           98 t
ORIGIN      
        1 atttacgtag ctcacgctta acagagaaaa tacttatata tcaaaggctt ggttaaaaat
       61 gattaaaatg cactaagtct ttgaaatcaa aagagaattt aatgatttac tctaatcagc
      121 ttcgttacac tggcaaagat ggtgtttact tccatctaca aggccaacca gataatttct
      181 tgtcactatc agcaactgaa aacatggaat ttgatagccc tatgatggtt accactcaaa
      241 atatgcaatc agggcagact gtaacagata atgtaatgcg ttcacctaga actatttcaa
      301 tcagtggtgt ggttgtggtt ggtg
//
LOCUS       DAJTMX010000410          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTMX010000410 DAJTMX010000000
VERSION     DAJTMX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.418"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..248)
                     /locus_tag="OO145_004472"
     rRNA            complement(<1..248)
                     /locus_tag="OO145_004472"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           70 a           88 c           67 g           98 t
ORIGIN      
        1 ccgctactgg gggaatctcg gttgatttct tttcctcggg gtacttagat gtttcagttc
       61 ccccggttcg cctcattaac ctatggattc agttaatgat agtgtgacga atcacactgg
      121 gtttccccat tcggaaatcg ccggttataa cggttcatat caccttaccg acgcttatcg
      181 cagattagca cgtccttcat cgcctctgac tgccagggca tccaccgtgt acgcttagtc
      241 gcttaacctc acaacccgaa gatgtttctt tcgactcatc atcgtgttgc gaaaatttga
      301 gagactcacg aacaactttc gtt
//
LOCUS       DAJTMX010000411          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTMX010000411 DAJTMX010000000
VERSION     DAJTMX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.419"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>311)
                     /locus_tag="OO145_004473"
     CDS             complement(<1..>311)
                     /locus_tag="OO145_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5469306.1"
                     /translation="LVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFF
                     QLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGS
                     V"
BASE COUNT           74 a           84 c           61 g           92 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc taccctccag
      241 gccgtgtgat ttcaggaacg actgccactc atggcttgtg tactgactgc cctgatccga
      301 atgaaccagc a
//
LOCUS       DAJTMX010000412          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTMX010000412 DAJTMX010000000
VERSION     DAJTMX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.420"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>304
                     /locus_tag="OO145_004474"
     CDS             <1..>304
                     /locus_tag="OO145_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469307.1"
                     /translation="SAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAID
                     GVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSEN"
BASE COUNT           66 a          101 c           88 g           49 t
ORIGIN      
        1 agtgccgtta ttcaggcccg ccagcgcctg ggaagtgagg ctgtccgccg cgtgttcacg
       61 aaaacagcgc agctctggca taacgccacg ccgcatccgc actggtgcgg cctgaccctg
      121 ctggccatcg atggtgtgtt ctggcgcaca ccggatacac cagagaacga tgcagccttc
      181 ccccgccaga cacatgccgg gaacccggcg ctctacccgc aggtcaaaat ggtctgccag
      241 atggaactga ccagccatct gctgacggct gcagccttcg gcacgatgaa gaacagcgaa
      301 aatg
//
LOCUS       DAJTMX010000413          302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTMX010000413 DAJTMX010000000
VERSION     DAJTMX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.421"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            62..>302
                     /locus_tag="OO145_004475"
     CDS             62..>302
                     /locus_tag="OO145_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCR5469308.1"
                     /translation="MAELAIELDDMTIITEKLRDMKNALSKLNHDIEEDTKRIKAITT
                     GSLKDIDRTTKEGMVETQLLIKGVLKEIVIDAAKRR"
BASE COUNT          117 a           55 c           64 g           66 t
ORIGIN      
        1 tactgacact attgatagcc ttaaattaca gcgtgaagat ttgcagcgta aaatctcaaa
       61 aatggcagaa cttgctattg aactcgatga tatgacaatt atcacagaga aattacgcga
      121 catgaagaat gcgctttcaa aactcaatca tgatatcgaa gaggatacaa agcggataaa
      181 ggccatcaca actggaagcc tcaaagatat tgacaggaca acaaaggaag ggatggtaga
      241 gacacagcta cttatcaagg gcgtactcaa agaaatagtt atagatgccg ctaaacgtcg
      301 at
//
LOCUS       DAJTMX010000414          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTMX010000414 DAJTMX010000000
VERSION     DAJTMX010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.422"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>300
                     /locus_tag="OO145_004476"
     CDS             <1..>300
                     /locus_tag="OO145_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HCR5469309.1"
                     /translation="YAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWP
                     LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY"
BASE COUNT           69 a           71 c           95 g           65 t
ORIGIN      
        1 tacgcgtatg acaggctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg
       61 gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg
      121 gatggctggc cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga
      181 tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga
      241 cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
//
LOCUS       DAJTMX010000415          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTMX010000415 DAJTMX010000000
VERSION     DAJTMX010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.423"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           70 a           60 c           76 g           89 t
ORIGIN      
        1 ttagtctttg catgagcaag cggactgaca catttctctt tgttctcatt cagaaaatct
       61 catcagtcgg cgttcagacc ggtgttgtgc tttatggata cgttacgctc ctgatgacgt
      121 catttgacgt tcaacagcat cacggggccg cacgacattt cacgtcagtt agtgctatag
      181 ctcaggaaca aattttccca aattgggata tgctcgcaaa ttgctggtga tgtggggaga
      241 atctggttga gttcggtaga attgatttgg aggcagaacg cttaaatcgt ggcgt
//
LOCUS       DAJTMX010000416          294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTMX010000416 DAJTMX010000000
VERSION     DAJTMX010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.424"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>294)
                     /locus_tag="OO145_004477"
     rRNA            complement(<1..>294)
                     /locus_tag="OO145_004477"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           53 a          102 c           64 g           75 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttct
//
LOCUS       DAJTMX010000417          288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTMX010000417 DAJTMX010000000
VERSION     DAJTMX010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.425"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(55..243)
                     /locus_tag="OO145_004478"
     CDS             complement(55..243)
                     /locus_tag="OO145_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469310.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT           73 a           70 c           85 g           60 t
ORIGIN      
        1 cgtgaacaga cgtttcagcg ggcggggagt aaaagcggac aacatgagga cggattaacc
       61 cccggtatcc tgtagccggg taagggctgc tgtgtcgcca cggcgctcca ggatggaggc
      121 cgcgaccatc gcaggtggtg ggtccatggg tttgagcatc ttcatccagt cccattcacg
      181 ttgtgagagc ttctcagcac caacagagag cagaacggta tctatacttt tgcgggtcaa
      241 cataagagga tgagtcctta ataaaacagg tcctcagagc atacatat
//
LOCUS       DAJTMX010000418          287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTMX010000418 DAJTMX010000000
VERSION     DAJTMX010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.426"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..263
                     /locus_tag="OO145_004479"
     CDS             <1..263
                     /locus_tag="OO145_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR5469311.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT           81 a           61 c           63 g           82 t
ORIGIN      
        1 gttcaacagg aagaattctg gttctgagtc tgctggcaac gttgagcact atcgtaatgt
       61 ggttactcgg ttatcacgct gaaaataaag ggttacacct gaaatatcag gctaacagca
      121 ttaaatcccg gcgggttatc tcatatctga cgttagcgaa gaatgttctt cgacactcgc
      181 cacttatttt aagacgaaca gtactgagca cagttctaaa ccatctgtcc agaacctacc
      241 gaaatatggt gctggtttat tagcagtaat tttgtgggga tccctca
//
LOCUS       DAJTMX010000419          285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTMX010000419 DAJTMX010000000
VERSION     DAJTMX010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.427"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>285
                     /locus_tag="OO145_004480"
     CDS             <1..>285
                     /locus_tag="OO145_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5469312.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSP"
BASE COUNT           66 a           72 c           92 g           55 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gccca
//
LOCUS       DAJTMX010000420          281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTMX010000420 DAJTMX010000000
VERSION     DAJTMX010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.428"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>281
                     /locus_tag="OO145_004481"
     CDS             <1..>281
                     /locus_tag="OO145_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5469313.1"
                     /translation="QRQKEFQQAYANAYASGDRGALRQLATQYPDQIESVRKGMGFID
                     EEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVAQMY"
BASE COUNT           67 a           69 c           75 g           70 t
ORIGIN      
        1 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
       61 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
      121 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
      181 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
      241 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc a
//
LOCUS       DAJTMX010000421          277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTMX010000421 DAJTMX010000000
VERSION     DAJTMX010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.429"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            1..277
                     /locus_tag="OO145_004482"
                     /pseudo
     CDS             1..277
                     /locus_tag="OO145_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           86 a           58 c           89 g           44 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgtt
//
LOCUS       DAJTMX010000422          275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTMX010000422 DAJTMX010000000
VERSION     DAJTMX010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.430"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>275
                     /locus_tag="OO145_004483"
     CDS             <1..>275
                     /locus_tag="OO145_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5469314.1"
                     /translation="VNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLR
                     PLGIPALRDRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTV"
BASE COUNT           63 a           79 c           75 g           58 t
ORIGIN      
        1 gtgaacaaaa caatgctaca ggccagactg gctgttgagc tgcaaatcct cagggatgaa
       61 ttactctcag gccactacca gcccttgccc gccagacggg tttacatccc taaaagcaac
      121 ggcaaactgc gaccactggg tatccccgcg ttgcgggatc gtattgttca gcgggccatg
      181 ctgatggcga tggagccgat atgggagagt gattttcata cgctctcata tggcttccgg
      241 cctgagcgca gtgtccacca cgcgatccgc acggt
//
LOCUS       DAJTMX010000423          274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTMX010000423 DAJTMX010000000
VERSION     DAJTMX010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.431"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..244)
                     /locus_tag="OO145_004484"
     CDS             complement(<1..244)
                     /locus_tag="OO145_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR5469315.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPL"
BASE COUNT           62 a           75 c           63 g           74 t
ORIGIN      
        1 gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg gtatccgata
       61 tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa acggtgttac
      121 tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc tctgttcgat
      181 atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg ttaacagtga
      241 acactatgct gttatccggg tttctctggc tgaa
//
LOCUS       DAJTMX010000424          272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTMX010000424 DAJTMX010000000
VERSION     DAJTMX010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.432"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(207..>272)
                     /locus_tag="OO145_004485"
     CDS             complement(207..>272)
                     /locus_tag="OO145_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5469316.1"
                     /translation="EVRIRTGQTEVAVFVDYESRK"
BASE COUNT           63 a           77 c           74 g           58 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tc
//
LOCUS       DAJTMX010000425          258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTMX010000425 DAJTMX010000000
VERSION     DAJTMX010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.433"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           79 a           66 c           43 g           70 t
ORIGIN      
        1 ccatctgata catatcccgc ccccccgaca gacctgtgct atcggaaaga aaaaaggcca
       61 tcagcaaaac tctgatggcc tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg
      121 actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc ttaattcatg
      181 aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa tgtttttgct
      241 ggcgggtcct tttttcta
//
LOCUS       DAJTMX010000426          258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTMX010000426 DAJTMX010000000
VERSION     DAJTMX010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.434"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>258
                     /locus_tag="OO145_004486"
     CDS             <1..>258
                     /locus_tag="OO145_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469317.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT           67 a           71 c           71 g           49 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaat
//
LOCUS       DAJTMX010000427          256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTMX010000427 DAJTMX010000000
VERSION     DAJTMX010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.435"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..185
                     /locus_tag="OO145_004487"
     CDS             <1..185
                     /locus_tag="OO145_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5469318.1"
                     /translation="VLFGKTNHGKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRN
                     TYTSRFTEVAMAFASI"
BASE COUNT           68 a           57 c           82 g           49 t
ORIGIN      
        1 gggttttatt tggtaaaacc aatcacggca agctcagcgg cgaaatactg taccggttgg
       61 tggggcaagg caagaagctg ttccgctggc ggctacccga aggtaatccc tatctgagga
      121 cggagaccag aaacacgtat acatcgcgct ttacagaagt ggcaatggcg ttcgccagca
      181 tttaaatgga gagccggatg cgctgaaagg tgcacgtccg gttcggggag gagaggcagg
      241 gaaatagtcc gactac
//
LOCUS       DAJTMX010000428          253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTMX010000428 DAJTMX010000000
VERSION     DAJTMX010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.436"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>253
                     /locus_tag="OO145_004488"
     CDS             <1..>253
                     /locus_tag="OO145_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469319.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG"
BASE COUNT           63 a           73 c           59 g           58 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga
      241 cttacacgcc ggt
//
LOCUS       DAJTMX010000429          249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTMX010000429 DAJTMX010000000
VERSION     DAJTMX010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.437"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           63 a           76 c           53 g           57 t
ORIGIN      
        1 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
       61 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      121 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      181 catccgggcc agcggctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      241 ccccgtaaa
//
LOCUS       DAJTMX010000430          248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTMX010000430 DAJTMX010000000
VERSION     DAJTMX010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.438"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>248
                     /locus_tag="OO145_004489"
     CDS             <1..>248
                     /locus_tag="OO145_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5469320.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSR"
BASE COUNT           60 a           64 c           70 g           54 t
ORIGIN      
        1 gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg aagcccgcgg
       61 gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca gttcaatccg
      121 gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca cgaaggctgg
      181 ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa
      241 tcccggat
//
LOCUS       DAJTMX010000431          243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTMX010000431 DAJTMX010000000
VERSION     DAJTMX010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.439"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            39..>243
                     /locus_tag="OO145_004490"
     CDS             39..>243
                     /locus_tag="OO145_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5469321.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIR"
BASE COUNT           73 a           53 c           66 g           51 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gac
//
LOCUS       DAJTMX010000432          241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTMX010000432 DAJTMX010000000
VERSION     DAJTMX010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.440"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..230
                     /locus_tag="OO145_004491"
     CDS             <1..230
                     /locus_tag="OO145_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5469322.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT           66 a           53 c           69 g           53 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 a
//
LOCUS       DAJTMX010000433          241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTMX010000433 DAJTMX010000000
VERSION     DAJTMX010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.441"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..233
                     /locus_tag="OO145_004492"
     CDS             <1..233
                     /locus_tag="OO145_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5469323.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT           63 a           43 c           65 g           70 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 g
//
LOCUS       DAJTMX010000434          239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTMX010000434 DAJTMX010000000
VERSION     DAJTMX010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.442"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>239
                     /locus_tag="OO145_004493"
     CDS             <1..>239
                     /locus_tag="OO145_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469324.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEM"
BASE COUNT           58 a           57 c           74 g           50 t
ORIGIN      
        1 gtggcggtca ggtgcttgct gttccgccag cgtacacaag ccagcgttgc gcgtgctgtg
       61 gtcatacagc gaaagaaaat cgcctgtcac aaagtaaatt cagatgccag gtatgtggat
      121 atacagcgaa cgccgatgta aatggagctc gtaacatttt agcggcgggg cacgccgttc
      181 ttgcctgtgg agagatggtg cagtcaggcc gctcgttgaa gcaggaaccc accgaaatg
//
LOCUS       DAJTMX010000435          237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTMX010000435 DAJTMX010000000
VERSION     DAJTMX010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.443"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            complement(<1..>237)
                     /locus_tag="OO145_004494"
     CDS             complement(<1..>237)
                     /locus_tag="OO145_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469325.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT           44 a           80 c           50 g           63 t
ORIGIN      
        1 tcacgactca ggaactgttt caccatttgt gcatagcaca ctttcgccac tggttccggt
       61 ttatccatcc ccagagcacg gtacacctgc ggcagcttct ctccacacac ccggttggca
      121 aggaacccga agtacctcac catcttaaaa aacttctccg ggatgtgctg tttcagcctc
      181 gcgaccagct cacgctgtgt cagcgtttcc gtcgccgttt ctcccgtttt gtggtcc
//
LOCUS       DAJTMX010000436          236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTMX010000436 DAJTMX010000000
VERSION     DAJTMX010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.444"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>236
                     /locus_tag="OO145_004495"
     CDS             <1..>236
                     /locus_tag="OO145_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5469326.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWM
                     ESTIFPALNDVPALSGLIDTLNPLGFNYQRDNEMA"
BASE COUNT           55 a           59 c           67 g           55 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgctcag tacaccggaa aaatgacgga tgaagatgac
       61 tggcaggctg ttctgcatat tgccgtcttc atccgggcac aggcaccgga ttcagagctg
      121 gatatgtgga tggagagcac cattttcccg gccctgaatg atgtaccggc actttccgga
      181 ctcatcgaca ccctgaaccc actcggtttt aactatcaac gtgataatga gatggc
//
LOCUS       DAJTMX010000437          233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTMX010000437 DAJTMX010000000
VERSION     DAJTMX010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.445"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>233
                     /locus_tag="OO145_004496"
     CDS             <1..>233
                     /locus_tag="OO145_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5469327.1"
                     /translation="INAGLSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTET
                     SPFSGDAAAATNPAVHAAEARVAQGVPMTPATK"
BASE COUNT           68 a           59 c           58 g           48 t
ORIGIN      
        1 tattaatgcc ggacttagca ttgctacgca tggcctgata aatggcgtta cccgtggtgt
       61 tcgtggtgca tcaaatataa ttagtggcaa taaaacatct gcacagagag cgaccactgc
      121 gccaacagaa acatcaccat tctccggtga tgccgctgca gcaacaaatc ctgcggttca
      181 tgccgcagag gcaagagtag cacaaggtgt accaatgacg cctgcgacga aga
//
LOCUS       DAJTMX010000438          224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTMX010000438 DAJTMX010000000
VERSION     DAJTMX010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.446"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>224
                     /locus_tag="OO145_004497"
     CDS             <1..>224
                     /locus_tag="OO145_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5469328.1"
                     /translation="NGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLL
                     NQTFAPTAPNQVWVADLTYVATQEGWLYLAG"
BASE COUNT           55 a           61 c           63 g           45 t
ORIGIN      
        1 aatggcatca tcgttggtcg tgaccgactg gcacgtcttc gtaaggagct gaggctacgc
       61 tgtaagcaga aacgcaagtt cagagcgact acgaacccga accacaatct gccagttgcg
      121 ccaaatctgc tgaaccagac gttcgctcct acagcaccaa atcaggtctg ggtggcggac
      181 ctgacgtatg ttgccacaca ggagggatgg ttgtacctcg ctgg
//
LOCUS       DAJTMX010000439          224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTMX010000439 DAJTMX010000000
VERSION     DAJTMX010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.447"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            26..181
                     /locus_tag="OO145_004498"
                     /pseudo
     CDS             26..181
                     /locus_tag="OO145_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           74 a           50 c           52 g           48 t
ORIGIN      
        1 ctccaacaac ggagacaggt aaattatgga cgaaaagaag cttaaagccc ttgcggctga
       61 actggctaaa ggcctcaaaa ccgaggccga cctcaatcag ttttcccgta tgcagacgaa
      121 attaactgtc gaaacggtgc ttaatgcgga actaactgac catctcgggc atgaaaaaag
      181 ctacatccgg tagtaaacat tatcagtggg ttatgcgata tgat
//
LOCUS       DAJTMX010000440          219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTMX010000440 DAJTMX010000000
VERSION     DAJTMX010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.448"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            35..>219
                     /locus_tag="OO145_004499"
     CDS             35..>219
                     /locus_tag="OO145_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5469329.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAM"
BASE COUNT           52 a           59 c           49 g           59 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaa
//
LOCUS       DAJTMX010000441          212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTMX010000441 DAJTMX010000000
VERSION     DAJTMX010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.449"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           63 a           43 c           74 g           32 t
ORIGIN      
        1 ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg gctgaagaaa
       61 aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct aagtgtgccc
      121 gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac catgcagaga
      181 gagaaaacac cggagtggag ggaaaagcag aa
//
LOCUS       DAJTMX010000442          209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTMX010000442 DAJTMX010000000
VERSION     DAJTMX010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.450"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           38 a           67 c           53 g           51 t
ORIGIN      
        1 gccccttcct cgggaggggc tttcccgttt cagccccttg ctgactcccc gattgttgat
       61 gagcgccgga ccccgcgcaa cgctttccct gaagcagttt tgccttcccc gagcggtcac
      121 gcaaaacgct gcaacgtcct gagcatttgc accttttagc ggaacgcaga tataaaaaat
      181 ccccggcttt ttgggccagg gatatgtgt
//
LOCUS       DAJTMX010000443          208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTMX010000443 DAJTMX010000000
VERSION     DAJTMX010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.451"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>208
                     /locus_tag="OO145_004500"
     CDS             <1..>208
                     /locus_tag="OO145_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5469330.1"
                     /translation="FHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPD
                     EGDLKPDVVKRFEEMVAQAQQSAGA"
BASE COUNT           51 a           47 c           67 g           43 t
ORIGIN      
        1 gtttccatgc gcggactgga aaatatggtg tgtatctgaa acaggactgg cgcaacgagt
       61 acaacgttgg cgacattgct gtatatgagg actcaaagcc cggcacgctg aatgactttc
      121 tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt gaggaaatgg
      181 tggcgcaggc gcagcagagc gccggggc
//
LOCUS       DAJTMX010000444          206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTMX010000444 DAJTMX010000000
VERSION     DAJTMX010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.452"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           63 a           34 c           41 g           68 t
ORIGIN      
        1 gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat atatttctcc
       61 atctatgact tgtcaatact atcctgatat ttgtgggtca gcaatatatt ctactaaaag
      121 gtatttaagg tcattgcgtc cctgcactcg aataatggtg aagaaagcat gataaatata
      181 tcacgataac gttcagtatg tatcga
//
LOCUS       DAJTMX010000445          203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTMX010000445 DAJTMX010000000
VERSION     DAJTMX010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.453"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..>203
                     /locus_tag="OO145_004501"
     CDS             <1..>203
                     /locus_tag="OO145_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5469331.1"
                     /translation="QKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQY
                     KAVEQAIKSRAGSALQVQENEAI"
BASE COUNT           60 a           47 c           46 g           50 t
ORIGIN      
        1 caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc tcaggctgaa
       61 gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca cttttccggg
      121 aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc tgctctacag
      181 gtgcaggaaa atgaagcaat cag
//
LOCUS       DAJTMX010000446          200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTMX010000446 DAJTMX010000000
VERSION     DAJTMX010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.denovo.454"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
BASE COUNT           57 a           44 c           57 g           42 t
ORIGIN      
        1 gcagagagct tcgtgaaaac gataaagcgt gactacatca gtatcatgcc caaaccagac
       61 gggttaacgg cagcaaagaa ccttgcagag gcgttcgagc attataacga atggcatccg
      121 catagtgcgc tgggttatcg ctcgccacgg gaatatctgc ggcagcgggc ttgtaatggg
      181 ttaagtgata acagatgtct
//
LOCUS       DAJTMX010000447         1777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.292, whole genome shotgun sequence.
ACCESSION   DAJTMX010000447 DAJTMX010000000
VERSION     DAJTMX010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.292"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            107..1312
                     /gene="tet(B)"
                     /locus_tag="OO145_004503"
     CDS             107..1312
                     /gene="tet(B)"
                     /locus_tag="OO145_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5469332.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1425..>1777)
                     /locus_tag="OO145_004504"
     CDS             complement(1425..>1777)
                     /locus_tag="OO145_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5469333.1"
                     /translation="LTNGWSALESIFEHYLDYIIDNNKNLIPIQEVMPIIGWNELEKI
                     SLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQF
                     IAIYRGFLLSLKDK"
BASE COUNT          454 a          325 c          380 g          618 t
ORIGIN      
        1 ctttactttt atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcatt
       61 gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac
      121 aaagatcgca ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt
      181 cttgccaacg ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt
      241 attgcttgca ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc
      301 tgaccgattt ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta
      361 cttattgctg gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg
      421 gatcacagga gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc
      481 tcaacgcgtg aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg
      541 gcctattatt ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc
      601 gttgctaaat attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac
      661 acgtgataat acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac
      721 tttatttaaa acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca
      781 aattcccgca acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat
      841 ggttggcttt tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc
      901 aggaagaata gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga
      961 tagtagtgca tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt
     1021 aattttattg gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca
     1081 aacaaagagt catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac
     1141 cggtgttatt ggcccattac tgtttgctgt tatttataat cattcactac caatttggga
     1201 tggctggatt tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac
     1261 cttcatgtta acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg
     1321 tcaccaaatg atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt
     1381 ttttacgata aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag
     1441 ctcaataaaa agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt
     1501 aggtttttcg catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca
     1561 tcatcatcat aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc
     1621 ttaccagtaa tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc
     1681 acttcttgga tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca
     1741 aatatacttt ctaaggcaga ccaaccattt gttaaat
//
LOCUS       DAJTMX010000448         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.303, whole genome shotgun sequence.
ACCESSION   DAJTMX010000448 DAJTMX010000000
VERSION     DAJTMX010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.303"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..216
                     /locus_tag="OO145_004505"
     CDS             <1..216
                     /locus_tag="OO145_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HCR5469334.1"
                     /translation="ALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKI
                     HVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            279..1409
                     /locus_tag="OO145_004506"
     CDS             279..1409
                     /locus_tag="OO145_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5469335.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
BASE COUNT          385 a          417 c          397 g          390 t
ORIGIN      
        1 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
       61 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
      121 gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
      181 acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc
      241 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
      301 ccttattaat taccgcctct tactccacat ttgctgcccc tcaacaaatc aacgatattg
      361 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
      421 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
      481 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
      541 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat
      601 actggcctga acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct
      661 acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc
      721 tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca
      781 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
      841 aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
      901 ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt
      961 cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc
     1021 gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt caacaaggga
     1081 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     1141 aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac aataaaattg
     1201 cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta cgcgcatcat
     1261 gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa
     1321 aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     1381 cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt
     1441 tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg
     1501 cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagtttt
     1561 cctacgccgc cgcgtggcgt gaccgtagt
//
LOCUS       DAJTMX010000449         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.304, whole genome shotgun sequence.
ACCESSION   DAJTMX010000449 DAJTMX010000000
VERSION     DAJTMX010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.304"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            <1..216
                     /locus_tag="OO145_004507"
     CDS             <1..216
                     /locus_tag="OO145_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HCR5469336.1"
                     /translation="ALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKI
                     HVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            279..1409
                     /locus_tag="OO145_004508"
     CDS             279..1409
                     /locus_tag="OO145_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5469337.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALSARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
BASE COUNT          385 a          417 c          396 g          391 t
ORIGIN      
        1 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
       61 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
      121 gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
      181 acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc
      241 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
      301 ccttattaat taccgcctct tactccacat ttgctgcccc tcaacaaatc aacgatattg
      361 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
      421 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
      481 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
      541 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat
      601 actggcctga acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct
      661 acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc
      721 tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca
      781 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
      841 aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
      901 ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt
      961 cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc
     1021 gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt caacaaggga
     1081 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     1141 aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac aataaaattg
     1201 cactgtcagc acgcccggta aaagcgatta agcccccaac tcctgcagta cgcgcatcat
     1261 gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa
     1321 aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     1381 cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt
     1441 tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg
     1501 cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagtttt
     1561 cctacgccgc cgcgtggcgt gaccgtagt
//
LOCUS       DAJTMX010000450         1206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.319, whole genome shotgun sequence.
ACCESSION   DAJTMX010000450 DAJTMX010000000
VERSION     DAJTMX010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.319"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            25..855
                     /locus_tag="OO145_004509"
     CDS             25..855
                     /locus_tag="OO145_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5469338.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            968..>1206
                     /locus_tag="OO145_004510"
     CDS             968..>1206
                     /locus_tag="OO145_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR5469339.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGE"
BASE COUNT          420 a          249 c          231 g          306 t
ORIGIN      
        1 ccctttacca aaccaatact tattatgaaa aacacaatac atatcaactt cgctattttt
       61 ttaataattg caaatattat ctacagcagc gccagtgcat caacagatat ctctactgtt
      121 gcatctccat tatttgaagg aactgaaggt tgttttttac tttacgatgc atccacaaac
      181 gctgaaattg ctcaattcaa taaagcaaag tgtgcaacgc aaatggcacc agattcaact
      241 ttcaagatcg cattatcact tatggcattt gatgcggaaa taatagatca gaaaaccata
      301 ttcaaatggg ataaaacccc caaaggaatg gagatctgga acagcaatca tacaccaaag
      361 acgtggatgc aattttctgt tgtttgggtt tcgcaagaaa taacccaaaa aattggatta
      421 aataaaatca agaattatct caaagatttt gattatggaa atcaagactt ctctggagat
      481 aaagaaagaa acaacggatt aacagaagca tggctcgaaa gtagcttaaa aatttcacca
      541 gaagaacaaa ttcaattcct gcgtaaaatt attaatcaca atctcccagt taaaaactca
      601 gccatagaaa acaccataga gaacatgtat ctacaagatc tggataatag tacaaaactg
      661 tatgggaaaa ctggtgcagg attcacagca aatagaacct tacaaaacgg atggtttgaa
      721 gggtttatta taagcaaatc aggacataaa tatgtttttg tgtccgcact tacaggaaac
      781 ttggggtcga atttaacatc aagcataaaa gccaagaaaa atgcgatcac cattctaaac
      841 acactaaatt tataaaaaat ctaatggcaa aatcgcccaa cccttcaatc aagtcgggac
      901 ggccaaaagc aagcttttgg ctcccctcgc tggcgctcgg cgccccttat ttcaaacgtt
      961 aaacatcatg agggaagtgg tgatcgccga agtatcgact caactatcag aggtagttgg
     1021 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     1081 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     1141 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     1201 agagag
//
LOCUS       DAJTMX010000451          996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.331, whole genome shotgun sequence.
ACCESSION   DAJTMX010000451 DAJTMX010000000
VERSION     DAJTMX010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.331"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            156..947
                     /gene="aadA1"
                     /locus_tag="OO145_004511"
     CDS             156..947
                     /gene="aadA1"
                     /locus_tag="OO145_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5469340.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          256 a          254 c          261 g          225 t
ORIGIN      
        1 caagaaaaat gcgatcacca ttctaaacac actaaattta taaaaaatct aatggcaaaa
       61 tcgcccaacc cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg
      121 gcgctcggcg ccccttattt caaacgttaa acatcatgag ggaagtggtg atcgccgaag
      181 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
      241 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
      301 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
      361 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
      421 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
      481 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
      541 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
      601 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
      661 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
      721 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
      781 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
      841 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
      901 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
      961 gttcaagccg acgccgcttc gcggcgcggc ttaact
//
LOCUS       DAJTMX010000452          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.341, whole genome shotgun sequence.
ACCESSION   DAJTMX010000452 DAJTMX010000000
VERSION     DAJTMX010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.341"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            154..813
                     /locus_tag="OO145_004512"
     CDS             154..813
                     /locus_tag="OO145_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5469341.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          230 a          185 c          201 g          248 t
ORIGIN      
        1 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
       61 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
      121 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      181 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      241 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      301 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      361 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      421 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      481 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      541 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
      601 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
      661 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
      721 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
      781 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
      841 taaacgcctg gttgctacgc ctga
//
LOCUS       DAJTMX010000453          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.342, whole genome shotgun sequence.
ACCESSION   DAJTMX010000453 DAJTMX010000000
VERSION     DAJTMX010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.342"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            154..813
                     /locus_tag="OO145_004513"
     CDS             154..813
                     /locus_tag="OO145_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5469342.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDCFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          230 a          185 c          200 g          249 t
ORIGIN      
        1 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
       61 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
      121 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      181 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      241 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      301 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      361 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      421 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      481 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      541 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
      601 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
      661 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
      721 caggttcatc atgccgtttg tgattgcttc catgtcggca gaatgcttaa tgaattacaa
      781 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
      841 taaacgcctg gttgctacgc ctga
//
LOCUS       DAJTMX010000454          664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 200294
            SAMEA8903672-rid15820953.guided.360, whole genome shotgun sequence.
ACCESSION   DAJTMX010000454 DAJTMX010000000
VERSION     DAJTMX010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903672
            Sequence Read Archive: ERR6006201
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478831.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478831.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,514
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 377
            CDSs (without protein)            :: 377
            Pseudo Genes (ambiguous residues) :: 0 of 377
            Pseudo Genes (frameshifted)       :: 183 of 377
            Pseudo Genes (incomplete)         :: 95 of 377
            Pseudo Genes (internal stop)      :: 143 of 377
            Pseudo Genes (multiple problems)  :: 43 of 377
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903672-rid15820953.guided.360"
                     /serovar="S. flexneri 2b"
                     /isolate="200294"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-05-01"
     gene            47..505
                     /gene="gpt"
                     /locus_tag="OO145_004514"
     CDS             47..505
                     /gene="gpt"
                     /locus_tag="OO145_004514"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5469343.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            597..>664
                     /locus_tag="OO145_004515"
     CDS             597..>664
                     /locus_tag="OO145_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5469344.1"
                     /translation="MTQANLSETLFKPRFKHPETSTL"
BASE COUNT          160 a          168 c          168 g          168 t
ORIGIN      
        1 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
       61 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
      121 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggggcgtt
      181 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
      241 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
      301 cgttattgat gacctggtgg ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc
      361 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
      421 tgttgttgat atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt
      481 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcattgccgg gcgttgttct
      541 ttttaacttc aggcaggtta caatagtttc cagtaagtat tctggaggct gcatccatga
      601 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
      661 cgct
//
